For research use only.

Catalog No.S7863

96 publications

SC79 Chemical Structure

CAS No. 305834-79-1

SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.

Selleck's SC79 has been cited by 96 publications

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.
Akt [1]
In vitro

SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 MYXBdI9xfG:|aYOgZZN{[Xl? NVnrd3ZIPSEQvH3vcE9N NHPvdHdUSzhyIHLsc4NseyC4aYTlfIlvNWmwZIXj[YQh[XCxcITvd4l{ MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODd7N{KzOkc,OzB5OUeyN|Y9N2F-
HeLa MX;GeY5kfGmxbjDhd5NigQ>? NHvT[lhl\WO{ZXHz[YQhfGinIH71cYJmeiCxZjDheZRweGijZ3;zc41meyCrbnT1Z4VlKGK7IFjWTk1GKGmwIHPlcIx{ Mmq0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3M{SwNFEoRjNyNUO0NFAyRC:jPh?=
SH-SY5Y MlXRSpVv[3Srb36gZZN{[Xl? MYCxNEDPxE1? NIi2Nm8{OCCvaX6= M3K4[ZBz\S22cnXheI1mdnRid3n0bEBUSzd7IDixNEDPxE1rIHzhdodmdHliYYT0[Y52[XSnZDDINm8zNWmwZIXj[YQhe3W{dnn2ZYwhemWmdXP0bY9v NWrZXIxURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlAxQTdpPkK5OVYxODl5PD;hQi=>
Sertoli NEjFV2RHfW6ldHnvckBie3OjeR?= M4X6RVUvPeLCidM1US=> MknxN|AhdWmw NFfpTYl2eC2{ZXf1cIF1\SC2aHWgVGZQWy2rbnT1Z4VlKGSxd36tdoVofWyjdHnvckBw\iCyLVHreFEhXDNyODDhcoQheC2Da4SxJHM1PzN? NEO1WpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESzPVA3Pyd-Mki0N|kxPjd:L3G+

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-mTOR / mTOR / pAKT / AKT / P62 / Bcl-2 / Bax / LC3 / Cleaved PARP ; 

PubMed: 30301881     

C643 and KHM-5M cells were treated by apatinib (20 μM) implement with or without SC79 (4 μg/ml) for 24 h, respectively. Total AKT, phosphorylated AKT, total mTOR, phosphorylated AKT, P62, Bcl-2, Bax, LC3, cleaved-PARP, and GAPDH were detected by western blot. Densitometry represents the expression of the proteins relative to GAPDH. 

p-Akt / Akt / p-GSK3β / GSK3β / β-catenin / Cyclin D1; 

PubMed: 30556279     

The relative expression levels of phosphorylated Akt308, Akt, phosphorylated GSK-3b, GSK-3b, bcatenin, and cyclin D1 with respective control cells. GAPDH was used as a loading control.

p4EBP1 / 4EBP1 / pS6 / RPS6 / ESRP1 / ESRP2; 

PubMed: 28420690     

Western blot analyses of the phosphorylation pattern of proteins involved downstream of the AKT signaling pathway in epithelial-like MCF-7 cells transfected with control siRNAs or siRNAs targeting ESRP1 and ESRP2 and treated, or not, for 1 h with SC79 (AKT kinase activator). The p4EBP1(70) and p4EBP1(37&46) antibodies recognize phosphorylated residues on position 70, 37 and/or 46 of the E4BP1 protein. The pS6 antibody recognizes phosphorylated RPS6 protein. H3 (histone H3) is used as a loading control. 

30301881 30556279 28420690
In vivo In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1]


Kinase Assay:


- Collapse

Cytosolic phosphorylation of Akt:

Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.
Cell Research:


- Collapse
  • Cell lines: HsSultan and NB4 cells
  • Concentrations: 8 μg/mL
  • Incubation Time: 24 h
  • Method:

    HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.

    (Only for Reference)
Animal Research:


- Collapse
  • Animal Models: Permanent focal cerebral ischemia mouse model
  • Dosages: 0.04 mg/g
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (197.37 mM)
Ethanol 72 mg/mL warmed (197.37 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 364.78


CAS No. 305834-79-1
Storage powder
in solvent
Synonyms N/A
Smiles CCOC(=O)C1=C(OC2=C(C1C(C#N)C(=O)OCC)C=C(C=C2)Cl)N

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Akt Signaling Pathway Map

Related Akt Products

Tags: buy SC79|SC79 ic50|SC79 price|SC79 cost|SC79 solubility dmso|SC79 purchase|SC79 manufacturer|SC79 research buy|SC79 order|SC79 mouse|SC79 chemical structure|SC79 mw|SC79 molecular weight|SC79 datasheet|SC79 supplier|SC79 in vitro|SC79 cell line|SC79 concentration|SC79 nmr|SC79 in vivo|SC79 clinical trial|SC79 inhibitor|SC79 PI3K/Akt/mTOR inhibitor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID