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SC79

Name: SC79
Brand: Selleck Chemicals
SKU: S7863
Rating: 5 (103 reviews)

Catalog No.S7863

For research use only.

SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.

CAS No. 305834-79-1

Selleck's SC79 has been cited by 103 publications

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Akt Signaling Pathway Map

Biological Activity

Description

SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.

Targets

Akt ^[1]

In vitro

SC79 suppresses PH_AKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. ^[1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

A549	M2D1[2Fxd3C2b4Ppd{Bie3OjeR?=	M4fFV\|Uh\|ryvb3yvUC=>		MU\TR\|gxKGKub3Prd{B3cXSneHnuMYlv\HWlZXSgZZBweHSxc3nz	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODd7N{KzOkc,OzB5OUeyN\|Y9N2F-
HeLa	NIXXVI5HfW6ldHnvckBie3OjeR?=			MmDm[IVkemWjc3XkJJRp\SCwdX3i[ZIhd2ZiYYX0c5Bp[Wexc3;t[ZMhcW6mdXPl[EBjgSCKVlqtSUBqdiClZXzsdy=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODV\|NECwNUc,OzB3M{SwNFE9N2F-
SH-SY5Y	NUe4TG1vTnWwY4Tpc44h[XO\|YYm=	NYnPUnI2OTBizszN	MnjCN\|AhdWmw	M2D6WZBz\S22cnXheI1mdnRid3n0bEBUSzd7IDixNEDPxE1rIHzhdodmdHliYYT0[Y52[XSnZDDINm8zNWmwZIXj[YQhe3W{dnn2ZYwhemWmdXP0bY9v	NInFV4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2NFA6Pyd-Mkm1OlAxQTd:L3G+
Sertoli	M{LaZWZ2dmO2aX;uJIF{e2G7	NU\6S3ViPS534pEJxtVO	MYOzNEBucW5?	M37i[pVxNXKnZ4XsZZRmKHSqZTDQSm9UNWmwZIXj[YQh\G:5bj3y[Yd2dGG2aX;uJI9nKHBvQXv0NUBVOzB6IHHu[EBxNUGtdEGgV\|Q4Ow>?	M1r4OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEO5NFY4Lz5{OESzPVA3PzxxYU6=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot

p-mTOR / mTOR / pAKT / AKT / P62 / Bcl-2 / Bax / LC3 / Cleaved PARP ; p-Akt / Akt / p-GSK3β / GSK3β / β-catenin / Cyclin D1 ; p4EBP1 / 4EBP1 / pS6 / RPS6 / ESRP1 / ESRP2

30301881 30556279 28420690

In vivo

In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. ^[1]

Protocol (from reference)

Kinase Assay: ^[1]	Cytosolic phosphorylation of Akt: Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl₂, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.
Cell Research: ^[1]	Cell lines: HsSultan and NB4 cells Concentrations: 8 μg/mL Incubation Time: 24 h Method: HsSultan or NB4 cells (2.5 × 10⁵) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.
Animal Research: ^[1]	Animal Models: Permanent focal cerebral ischemia mouse model Dosages: 0.04 mg/g Administration: i.p.

References

Solubility (25°C)

In vitro	DMSO	72 mg/mL (197.37 mM)
	Ethanol	72 mg/mL warmed (197.37 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+corn oil For best results, use promptly after mixing. Click to purchase: Corn Oil	5mg/mL

Chemical Information

Molecular Weight	364.78
Formula	C₁₇H₁₇ClN₂O₅
CAS No.	305834-79-1
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CCOC(=O)C1=C(OC2=C(C1C(C#N)C(=O)OCC)C=C(C=C2)Cl)N

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In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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