SC79

Catalog No.S7863

SC79 Chemical Structure

Molecular Weight(MW): 364.78

SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.

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Cited by 26 Publications

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Biological Activity

Description SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.
Targets
Akt [1]
In vitro

SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 M3ixWWFxd3C2b4Ppd{Bie3OjeR?= M4jGT|Uh|ryvb3yvUC=> NGGyTVBUSzhyIHLsc4NseyC4aYTlfIlvNWmwZIXj[YQh[XCxcITvd4l{ NXHHUmR4OzB5OUeyN|Y>
HeLa NWPlWlIzTnWwY4Tpc44h[XO|YYm= MVfk[YNz\WG|ZXSgeIhmKG63bXLldkBw\iCjdYTvdIhi\2:|b33ld{BqdmS3Y3XkJIJ6KEiYSj3FJIlvKGOnbHzz NUjHeHE5OzB3M{SwNFE>
SH-SY5Y M2rTW2Z2dmO2aX;uJIF{e2G7 MV6xNEDPxE1? MYWzNEBucW5? MYjwdoUufHKnYYTt[Y51KHerdHigV2M4QSBqMUCg{txOMSCuYYLn[Yx6KGG2dHXueYF1\WRiSELPNk1qdmS3Y3XkJJN2en[rdnHsJJJm\HWldHnvci=> M4nMNVI6PTZyMEm3
Sertoli NFjxb|BHfW6ldHnvckBie3OjeR?= MVi1MlXjiIoEtV2= MlfMN|AhdWmw MYn1dE1z\We3bHH0[UB1cGViUF\PV{1qdmS3Y3XkJIRwf25vcnXneYxifGmxbjDv[kBxNUGtdEGgWFMxQCCjbnSgdE1Cc3RzIGO0O|M> Mn\KNlg1OzlyNke=

... Click to View More Cell Line Experimental Data
In vivo In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1]

Protocol

Kinase Assay:

[1]
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Cytosolic phosphorylation of Akt:

Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.
Cell Research:

[1]
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  • Cell lines: HsSultan and NB4 cells
  • Concentrations: 8 μg/mL
  • Incubation Time: 24 h
  • Method:

    HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.


    (Only for Reference)
Animal Research:

[1]
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  • Animal Models: Permanent focal cerebral ischemia mouse model
  • Formulation: DMSO
  • Dosages: 0.04 mg/g
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (197.37 mM)
Ethanol 72 mg/mL warmed (197.37 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing. 		5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 364.78
Formula

C17H17ClN2O5
CAS No. 305834-79-1
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID