Catalog No.S7863

For research use only.

SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.

SC79 Chemical Structure

CAS No. 305834-79-1

Selleck's SC79 has been cited by 110 publications

Purity & Quality Control

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Biological Activity

Description SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.
Akt [1]
In vitro

SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. [1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A549 MXjBdI9xfG:|aYOgZZN{[Xl? NX3OUnhyPSEQvH3vcE9N MVzTR|gxKGKub3Prd{B3cXSneHnuMYlv\HWlZXSgZZBweHSxc3nz NWPWSpB5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3PVczOzZpPkOwO|k4OjN4PD;hQi=>
HeLa NVv1[Vg{TnWwY4Tpc44h[XO|YYm= M3fpSIRm[3KnYYPl[EB1cGViboXtZoVzKG:oIHH1eI9xcGGpb4PvcYV{KGmwZIXj[YQh[nliSG\KMWUhcW5iY3XscJM> NHf2d2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEWzOFAxOSd-M{C1N|QxODF:L3G+
SH-SY5Y NXnz[XZmTnWwY4Tpc44h[XO|YYm= MWOxNEDPxE1? NIL3e5U{OCCvaX6= NYfY[WdjeHKnLYTy[YF1dWWwdDD3bZRpKFOFN{mgLFExKM7:TTmgcIFz\2WueTDheJRmdnWjdHXkJGgzVzJvaX7keYNm\CC|dYL2bZZidCC{ZXT1Z5Rqd25? M3;Qe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NU[wNFk4Lz5{OUW2NFA6PzxxYU6=
Sertoli NGKwdXhHfW6ldHnvckBie3OjeR?= MWS1MlXjiIoEtV2= MnTmN|AhdWmw NXfrb2p7fXBvcnXneYxifGVidHjlJHBHV1NvaX7keYNm\CCmb4fuMZJm\3WuYYTpc44hd2ZicD3Bb5QyKFR|MEigZY5lKHBvQXv0NUBUPDd| NXXCZZhsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0N|kxPjdpPkK4OFM6ODZ5PD;hQi=>
Methods Test Index PMID
Western blot p-mTOR / mTOR / pAKT / AKT / P62 / Bcl-2 / Bax / LC3 / Cleaved PARP ; p-Akt / Akt / p-GSK3β / GSK3β / β-catenin / Cyclin D1 ; p4EBP1 / 4EBP1 / pS6 / RPS6 / ESRP1 / ESRP2 30301881 30556279 28420690
In vivo In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. [1]

Protocol (from reference)

Kinase Assay:


  • Cytosolic phosphorylation of Akt:

    Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.

Cell Research:


  • Cell lines: HsSultan and NB4 cells
  • Concentrations: 8 μg/mL
  • Incubation Time: 24 h
  • Method:

    HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.

Animal Research:


  • Animal Models: Permanent focal cerebral ischemia mouse model
  • Dosages: 0.04 mg/g
  • Administration: i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+corn oil
For best results, use promptly after mixing.


Chemical Information

Molecular Weight 364.78


CAS No. 305834-79-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCOC(=O)C1=C(OC2=C(C1C(C#N)C(=O)OCC)C=C(C=C2)Cl)N

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

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Handling Instructions

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