MG-132

Catalog No.S2619

MG-132 Chemical Structure

Molecular Weight(MW): 475.62

MG132 is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 and 1.2 μM for the inhibition of proteasome and calpain, respectively.

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Selleck's MG-132 had a noticeably greater effect when compared with similar products from Sigma: This is directly due to using the (S, R, S) - (-) - 2 configuration.

P21 protein can be degraded through ubiquitylation pathway. Inhibition of proteasome activities results in the accumulation of P21 protein, therefore, the more powerful the inhibiting effect, the more P21 protein is accumulated. (testing protein: P21; Cell line: Hela; treatment condition: final concentration of MG-132 is 5μM, with a 6 hour treatment time)

Cited by 54 Publications

11 Customer Reviews

  • Western blot of IκBζ expression in BMDMs treated with DI and stimulated with LPS for 1 h. MG132 or bafilomycin A (BafA) were added 30 min before LPS stimulation.

    Nature, 2018, 556(7702):501-504. MG-132 purchased from Selleck.

    MDA-MB-231 cells were treated with 10 uM MG132 and incubated under normoxia or hypoxia for 4 h. Endogenous interaction between LATS2 and SIAH2 was analysed by immunoprecipitation.

    Nat Cell Biol 2015 17(1), 95-103. MG-132 purchased from Selleck.

  • HeLa cells were transfected with control mimic, RMND5A siRNA or miR-138 mimic. After transfection for 36 h, cells were treated with proteasome inhibitor MG132 (20 uM) or Dimethyl sulfoxide (DMSO) for 8 h. Endogenous Exportin-5 protein and RMND5A protein expression was measured by western blotting.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

    HeLa cells were transfected with negative control mimic, miR-138 mimic or RMND5A siRNA. After transfection for 36 h, cells were treated with MG132 (20 uM) or DMSO for 8 h. RMND5A and Exportin-5 protein expression were analyzed by immunocytochemistry. Nuclei were counterstained with DAPI.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

  •  

    TFEB induced autophagy dependent ATZ clearance in vitro. MEF transiently co-transfected with either ATZ plus GFP expressing plasmids or ATZ plus TFEB expressing plasmids were incubated with proteasome inhibitor MG132 or with DMSO for 6 h. Upon MG132 treatment, steady state levels of 52 kDa ATZ band increased in both cells transfected with ATZ plus GFP and with ATZ plus TFEB expressing plasmids. Quantification of band intensities showed a 48% reduction of ATZ in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP, in the absence of the proteasome inhibitor. In MG132 treated cells, a 61% decrease of ATZ was detected in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP.

    EMBO Mol Med 2013 5, 397-412. MG-132 purchased from Selleck.

    JAK1 expression was analyzed in A375 cells expressing scrambled shRNA or shRNF125 (#2) and treated with MG132 (5 uM, 10 hr), and in Lu1205 cells expressing WT RNF125 and treated with MG132.

    Cell Rep 2015 11(9), 1458-73. MG-132 purchased from Selleck.

  • LRB1 and LRB2 regulate phyB protein levels. PhyB protein levels in crude extracts from the wild type (WT) and the indicated mutant backgrounds were assayed by immunoblot analysis with anti-phyB monoclonal antibodies. Anti-PBA1 and anti-histone H3 antibodies were used to confirm equal protein loading. Rc-induced loss of phyB in the wild type is inhibited by 100 uM MG132.

    Plant Physiol 2012 60(1), 118-34. MG-132 purchased from Selleck.

    Representative images for γ-H2AX-stained cells treated with 100 uM FA for 3 h without or with MG132. Fluorescence was measured in at least 50 nuclei in each of three experiments.

    Toxicol Appl Pharmacol 2015 286(2), 135-41. MG-132 purchased from Selleck.

  • Nek2A impedes SuFu ubiquitination. HEK293T cells transfected with Nek2A-Myc and Flag-SuFu for 48 h were treated with MG-132 to enrich ubiquitinated proteins, and subsequently subjected to co-immunoprecipitation assay. Precipitated proteins were probed using anti-Ub antibody.

    Int J Oncol, 2017, 50(2):373-380. MG-132 purchased from Selleck.

    GSK-3β phosphorylation at Ser9 is enhanced in NOK-overexpressed stable cell lines. The proteasome inhibitor, MG132, was added in medium of HeLa-HA and HeLa-NOK-HA cells for 12 h and then the p-GSK-3b(Ser9) was assayed by western blotting.

    FEBS Lett 2012 586, 3787–3792. MG-132 purchased from Selleck.

  • Truncated mouse Period2 (Per2) protein was expressed in NIH3T3 cells, treated by cycloheximide with or without MG132 for 1-4 hours.Per2 was reported to be degraded by the proteasome proteolysis. By adding MG132 (25 μM), a proteasome inhibitor, the degradation of Per2 was dramatically decreased within 4 hours treatment (shown by western blot).

    2012 Dr. Edison DUKE-NUS Graduate Medical School Singapore.. MG-132 purchased from Selleck.

Purity & Quality Control

Choose Selective Proteasome Inhibitors

Biological Activity

Description MG132 is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 and 1.2 μM for the inhibition of proteasome and calpain, respectively.
Targets
20S proteasome [9]
(Cell-free assay)
100 nM
In vitro

MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. MG-132 also inhibits calpain with IC50 of 1.2 μM. MG-132 induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. [1] MG-132 (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. [2] MG-132 treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. [3] Unlike BzLLLCOCHO or PS-341, MG-132 treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by MG-132 than BzLLLCOCHO. [4] MG-132 (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). [5] MG-132 significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LPS-stimulated RAW264.7 cells M{\XWGZ2dmO2aX;uJGF{e2G7 M{L6OVI2KM7:TR?= MoDLSG1UVw>? MV;Jcohq[mm2czDubZRzcWNib4jp[IUheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE4yKM7:TR?= NUTHfpVbOjR4OUe0PVY>
HL-60 NWXoR5I5S3m2b4TvfIlkKEG|c3H5 NELtN4o1OCEQvF2= M37vVlQ5KGh? NY\sR5NkTE2VTx?= Mlj2TWM2ODxzMDFOwG0> NX3GXYVYOjR4OUe0PVY>
SMMC-7721 NXjrRXJJS3m2b4TvfIlkKEG|c3H5 MVG0NEDPxE1? MoD1OFghcA>? MXLEUXNQ MlvPTWM2OD15LkGg{txO MVyyOFY6PzR7Nh?=
A-549 NHnNcnpEgXSxdH;4bYMhSXO|YYm= M2L3XFQxKM7:TR?= NWftVYpDPDhiaB?= MUTEUXNQ MV3JR|UxRDFyIN88US=> M4DwV|I1Pjl5NEm2
MCF-7 NYDSRo1OS3m2b4TvfIlkKEG|c3H5 MXq0NEDPxE1? M{XqZlQ5KGh? MXHEUXNQ MWDJR|UxRTdwMzFOwG0> NX\iWGM3OjR4OUe0PVY>
SW-480 M2XqOWN6fG:2b4jpZ{BCe3OjeR?= NEjyOZU1OCEQvF2= M{joXlQ5KGh? NIrYN5ZFVVOR NUjLS3pqUUN3ME20JO69VQ>? MmfwNlQ3QTd2OU[=
NCI-H929 M2i1S2N6fG:2b4jpZ{BCe3OjeR?= NFzoWlUyKM7:TR?= MkjlO|IhcA>? NVvKV49VTE2VTx?= NW\4ZmtsUUN3ME2wMlE4KM7:TR?= MmW1NlQ3OjVyOEi=
293T M4\1emN6fG:2b4jpZ{BCe3OjeR?= NEWwRoQyOCEQvF2= M{fnWlczKGh? MYrEUXNQ NH;CVW1KSzVyPEKg{txO NG[2dVMzPDZ{NUC4PC=>
293T Mm\PSpVv[3Srb36gRZN{[Xl? NE\heJAyOCEQvF2= Mmf3NlQhcA>? NYnyOmxCTE2VTx?= MXjNc4RmemG2ZXz5JIlv\HWlZYOgbIVifC2|aH;jb{Ah M3fmd|I1PjJ3MEi4
HeLa MYDLbY5ie2ViQYPzZZk> MkPONVAh|ryP NUnSXod{OSCq MlvMSG1UVw>? NYe2VoVPUW6mdXPld{BRSVKSIHPs[YF3[WenIHL5JIlvcGmkaYTpcocheHKxc3Xhd49u\SCjY4Tpeol1gQ>? NHrYPHkzPDN{MUizNy=>
MDA-MB-231 NVT6XGhnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTWZmYyKM7:TR?= MXW3NkBp NWnDbFlFTE2VTx?= NUXXb45zUUN3ME2wMlE5KM7:TR?= MkfWNlQyPTN{ME[=
MCF-7 NW\ze|R5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjFNplGOSEQvF2= MUe3NkBp Mm\2SG1UVw>? M2\iNGlEPTB;MD6xN{DPxE1? NXXLO2xLOjRzNUOyNFY>
MCF10A M{\5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkiyNUDPxE1? MWi3NkBp NVe2NGRmTE2VTx?= MVXJR|UxRTBwMkmg{txO NGLzRZozPDF3M{KwOi=>
HEK-293 M4nubmtqdmG|ZTDBd5NigQ>? MUeyJIFv\CB{MDFOwG0> NGLNdHUzKGh? NX\G[HkxTE2VTx?= NV7JNYV6UW6qaXLpeJMhS2iWLVygZYN1cX[rdImge4l1cCCLQ{WwJI9nKDlibl2g NWnzSI54OjN3NEC3PVA>
Calu6 MX7GeY5kfGmxbjDBd5NigQ>? MXyxNEDPxE1? NHLEWZYyQCCq MYjEUXNQ NVrMZXh4W2mpbnnmbYNidnSueTDhZ4N2dXWuYYTld{BnemG2YYjpckBxemWldYLzc5I> Mo\UNlM2ODZ2OE[=
IFN-gamma-induced RAW264.7 NV75[IZCTnWwY4Tpc44hSXO|YYm= NYrOR|dsTE2VTx?= NWW5S4g{UW6qaXLpeJMhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36ge4l1cCCLQ{WwJI9nKDBwMEe3JO69VQ>? MkP4NlIzPzd{N{e=
IPC227F NWnjOmRGS3m2b4TvfIlkKEG|c3H5 NHfrdnQyKM7:TR?= MWe0PEBp M3r1fGROW09? NY\YS4gyUUN3ME2wMlA4PyEQvF2= M2DLNVIyQTJ2NUK4
Hepa-1c1c7 MV;GeY5kfGmxbjDBd5NigQ>? MlzRNlUh|ryP NWrnRWZoPiCq Ml\CSG1UVw>? MnK3TY5kemWjc3XzJG5z\jJicILveIVqdiCuZY\lcC=> NXr3XXh5OjB|OUK1OFQ>
COS-7 MYTDfZRwfG:6aXOgRZN{[Xl? MljXNVAh|ryP Mn\BSG1UVw>? MnXHTWM2ODxzMDFOwG0> MkXINVgxQDhyOUe=
HuH-7 MYrDfZRwfG:6aXOgRZN{[Xl? NUmxVWpLOTBizszN NGX3XFBFVVOR MWXJR|UxRDFyIN88US=> M4PsVVE5ODh6MEm3
OCI-Ly3 NGnzRndHfW6ldHnvckBCe3OjeR?= M3W0blExKM7:TdMg Mn\KOEBp NH\YRYRFVVOR MkX6TY5lfWOnczDoeY1idiCLa3HwdIFD[WyyaHGgd5Ri[mmuaYrheIlwdiC5aYToJGVEPTBib3[gNUDPxE1? NFiySmEyQDB{NEGxNy=>
A2780 cDDP MVLBdI9xfG:|aYOgRZN{[Xl? MlfsNlQhcA>? M1HKXWROW09? NFPKdJJKdmS3Y3XzJIFxd3C2b4Ppd{BjgSCrbnjpZol1cW6pIGDUSW4h\GWpcnHkZZRqd25? NVj3dGtGOTd4OESwNVg>
LPS-stimulated RAW264.7 cells NXu1SVZYTnWwY4Tpc44hSXO|YYm= NWfKcXYyTE2VTx?= MX7Jcohq[mm2czDOSk1s[XCyYVKgSG5CKGKrbnTpcoc> NVHBO|htOTd2MEeyO|c>
PC12 MXjGeY5kfGmxbjDBd5NigQ>? NXnaXYhCOTByIN88UeKh MWSyOEBp NYK4c2dqTE2VTx?= MXHJcohq[mm2czC2MW9JTEFvIHHu[EBJOk9{LXnu[JVk\WRiY4n0c5RwgGmlaYT5 NWPlNoJrOTdzNUi0OVQ>
PC3 NWK1cG5LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nhbFIxKM7:TR?= NH3Nb3Y1QCCq NWfzOlFITE2VTx?= NG\neXJKSzVyPUCuOkDPxE1? Mln4NVY3QDZ3M{e=
LP-1 NYm2OXgxSXCxcITvd4l{KEG|c3H5 MXWzNFAhdk1? MoXXNlQhcA>? NV;6ZZR[TE2VTx?= M2m4R2lv\HWlZYOgZZBweHSxc3nzJIJ6KGmwY4LlZZNqdmdiY3zlZZZm\CCSQWLQJIxmfmWuIHHu[EBz\WS3Y3nu[{BO[2xvMTDwdo91\WmwIHzleoVt NXL6R4g4OTV7NUi1PFk>
ES6 MlLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTBwMEGzNFYh|ryP M3XNSXNCVkeHUh?=
A101D M1nWc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTBwMEOyO|kh|ryP MmHZV2FPT0WU
OCUB-M MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXr4XIt4UUN3ME2wMlA{PzN5IN88US=> MUjTRW5ITVJ?
LB2518-MEL MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDYTWM2OD1yLkCzO|Yh|ryP MmLSV2FPT0WU
SH-4 M1jCUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37HNWlEPTB;MD6wOFMyKM7:TR?= M1zoSnNCVkeHUh?=
KNS-42 Mkj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlmxTWM2OD1yLkC0OFQyKM7:TR?= M1zMdnNCVkeHUh?=
DSH1 M1rz[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XjZWlEPTB;MD6wOVI5QSEQvF2= NILXdIhUSU6JRWK=
NTERA-S-cl-D1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULx[ZhuUUN3ME2wMlA2Pzd{IN88US=> MkTUV2FPT0WU
D-542MG MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTBwMEW5OVch|ryP MWHTRW5ITVJ?
KS-1 MoXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnPdpl2UUN3ME2wMlA3PDV6IN88US=> NWfVcXJMW0GQR1XS
BL-41 NYD3cnB4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHmNpFKSzVyPUCuNFY6PDdizszN MoHEV2FPT0WU
LXF-289 NUn3WXNHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDZe4pqUUN3ME2wMlA4ODZ4IN88US=> NUfCbplyW0GQR1XS
D-247MG M13BZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTXRYJKSzVyPUCuNFczODlizszN MUDTRW5ITVJ?
MMAC-SF Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTBwMEeyOlgh|ryP MlG3V2FPT0WU
CP66-MEL M1rOUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTBwMEe1JO69VQ>? MVTTRW5ITVJ?
LB771-HNC MnjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnL3TWM2OD1yLkC4NFM3KM7:TR?= NVOxVXRSW0GQR1XS
no-10 M1;V[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTBwMEi5NFkh|ryP NHzPV3RUSU6JRWK=
A388 NFP3bYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwMEmwOlUh|ryP NILTdJZUSU6JRWK=
OPM-2 MmPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vXNWlEPTB;MD6xNFQ4PCEQvF2= MVXTRW5ITVJ?
OVCAR-4 M13tPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLjTWM2OD1yLkGwPVM5KM7:TR?= NVrwfXE4W0GQR1XS
HOP-62 NVvJe3A3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITWOolKSzVyPUCuNVA6PDlizszN Ml3NV2FPT0WU
ML-2 M{nLU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEPrU3NKSzVyPUCuNVE5ODZizszN MXXTRW5ITVJ?
UACC-257 M4rmdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLsfYZKSzVyPUCuNVE6OjdizszN Mnz5V2FPT0WU
NEC8 MkT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17HPGlEPTB;MD6xNVk6PSEQvF2= NX\KfJl4W0GQR1XS
ONS-76 NFPWOnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\mfWlEPTB;MD6xNlk{PSEQvF2= NF\vSIVUSU6JRWK=
KE-37 M{T6[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwMUOyO|gh|ryP MmX3V2FPT0WU
HT-144 NXm4cJhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LMNmlEPTB;MD6xN|g6PSEQvF2= M4jWdHNCVkeHUh?=
LB2241-RCC M{HLOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3v1dWlEPTB;MD6xOFI1PyEQvF2= M1fJSnNCVkeHUh?=
TE-5 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\6V28zUUN3ME2wMlE1Ojd6IN88US=> M4jRT3NCVkeHUh?=
KINGS-1 NEnPV5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTOTnRrUUN3ME2wMlE1PzlizszN MlrCV2FPT0WU
NCI-H69 NWTNRnJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\zTWM2OD1yLkG1NVQyKM7:TR?= M3TaZXNCVkeHUh?=
CAS-1 NVPxdlNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPQSm1KSzVyPUCuNVU1QDJizszN NFfteZlUSU6JRWK=
D-263MG MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4f0XmlEPTB;MD6xOlAxPiEQvF2= M3rpR3NCVkeHUh?=
A253 NYjZflNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXH4[|J6UUN3ME2wMlE3OTJ6IN88US=> NFfBNmdUSU6JRWK=
PF-382 NEjCW5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jDfWlEPTB;MD6xOlcxPiEQvF2= NXT0V3Q5W0GQR1XS
CESS MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjzc5lKSzVyPUCuNVcxPiEQvF2= MVjTRW5ITVJ?
MZ2-MEL M3\EWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37Bc2lEPTB;MD6xO|U2QSEQvF2= MYjTRW5ITVJ?
HEL NW\CN4prT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfjTWM2OD1yLkG4OVE4KM7:TR?= MmHPV2FPT0WU
D-392MG M1;ycWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\URnJRUUN3ME2wMlE6OTF3IN88US=> NYDPWVVZW0GQR1XS
SK-LMS-1 NEHRZ5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13HXGlEPTB;MD6xPVMxOyEQvF2= NH;hc2FUSU6JRWK=
GI-ME-N NX;mZpRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnofXNKSzVyPUCuNVk{ODZizszN M{OxU3NCVkeHUh?=
LB831-BLC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXDTWM2OD1yLkG5N|Qh|ryP NXflbnczW0GQR1XS
DU-4475 NFrjR5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3y1RmlEPTB;MD6xPVY2QCEQvF2= NWrqRWt2W0GQR1XS
IST-SL1 Mlv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vQZ2lEPTB;MD6yNFA6PCEQvF2= MkPxV2FPT0WU
GAK M13rb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFz2NWNKSzVyPUCuNlA2OzRizszN NYC1XWhtW0GQR1XS
EW-1 NFzVXHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHpTWM2OD1yLkKxNFQ4KM7:TR?= NIi0cG9USU6JRWK=
LAMA-84 NVrpXG83T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{WzN2lEPTB;MD6yNVg2OSEQvF2= NH\odY1USU6JRWK=
SK-UT-1 NGjsXZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlGwTWM2OD1yLkKyNFQ6KM7:TR?= NGLxUldUSU6JRWK=
VA-ES-BJ NFjLd3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjQTJhnUUN3ME2wMlIzOjV5IN88US=> NWPmUHJtW0GQR1XS
ACN M1Poc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPkeFlrUUN3ME2wMlIzPjN6IN88US=> MWXTRW5ITVJ?
SK-PN-DW MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPB[2hnUUN3ME2wMlI{OTlizszN NFvscY9USU6JRWK=
HD-MY-Z NVr2blNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3K3S2lEPTB;MD6yN|MxOyEQvF2= Mlr3V2FPT0WU
LB373-MEL-D NUnYfFQxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPu[FFLUUN3ME2wMlI1OTl6IN88US=> MoPOV2FPT0WU
COLO-829 NITaeYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnV[pBDUUN3ME2wMlI1OjV5IN88US=> NHXBR2xUSU6JRWK=
ES8 M1O1UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Wwc2lEPTB;MD6yOFc3OyEQvF2= MXvTRW5ITVJ?
RXF393 M4LPfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLJTWM2OD1yLkK1NFE2KM7:TR?= Ml7UV2FPT0WU
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EVSA-T MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkGyTWM2OD1yLkS5PVEyKM7:TR?= Mor6V2FPT0WU
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BB65-RCC MoTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4i3cWlEPTB;MD61OlgyOSEQvF2= NVP3c45mW0GQR1XS
NCI-H1882 M2fkVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\1TWM2OD1yLkW5NFM3KM7:TR?= M{nldXNCVkeHUh?=
TE-9 MlXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fiPGlEPTB;MD62NVA{OyEQvF2= NFrk[JBUSU6JRWK=
NCI-H2126 NXjW[Ix6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwNkK2Olkh|ryP MY\TRW5ITVJ?
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SW872 M1PIfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fkbWlEPTB;MD62OVczQSEQvF2= NEjheGtUSU6JRWK=
LS-513 M2K4c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zCVWlEPTB;MD62Olc2OSEQvF2= NVTEfFk5W0GQR1XS
NCI-H1355 NHHLRoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjnTWM2OD1yLk[4OlE6KM7:TR?= NV7obHM4W0GQR1XS
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NCI-SNU-5 MlrGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\IVHllUUN3ME2wMlY6PTR3IN88US=> MljtV2FPT0WU
SNU-C2B MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjY[JRRUUN3ME2wMlcxPzN7IN88US=> M{LDTHNCVkeHUh?=
GB-1 M{S3d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\RfWlEPTB;MD63NlEzPCEQvF2= MmHVV2FPT0WU
CTB-1 NUG2Vmd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXT2fZJZUUN3ME2wMlc4QTV3IN88US=> MX7TRW5ITVJ?
Becker MlH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jvd2lEPTB;MD63PVYzOSEQvF2= MVzTRW5ITVJ?
KM12 NYrLWWhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LQVmlEPTB;MD64OVQ3PiEQvF2= MkfGV2FPT0WU
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HCC2998 MmTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLPWJZKSzVyPUCuPVM5PTRizszN NF;ZepZUSU6JRWK=
TE-10 MmHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzoZXVKSzVyPUCuPVY1OyEQvF2= M{TS[XNCVkeHUh?=
SF126 NWGybHBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[5d45KSzVyPUCuPVg6PzFizszN M2nTVHNCVkeHUh?=
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KURAMOCHI MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrkTWM2OD1zLkGyN|c1KM7:TR?= MVXTRW5ITVJ?
LU-65 M{PNXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnZVVZWUUN3ME2xMlEzPzV|IN88US=> NVnWepRkW0GQR1XS
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NCI-H23 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonDTWM2OD1zLkKwOFEh|ryP NGTncVZUSU6JRWK=
IA-LM MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTHTWM2OD1zLkK0NVE3KM7:TR?= NVW1[3c{W0GQR1XS
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NCI-H719 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTFwMke0NVEh|ryP NGO2cnZUSU6JRWK=
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EW-16 MnXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jITmlEPTB;MT6zNVM1PyEQvF2= M1rWdHNCVkeHUh?=
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DMS-114 NXHCbZBKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPvfYY{UUN3ME2xMlQ1PTN7IN88US=> MWDTRW5ITVJ?
GI-1 MoPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPXW|B6UUN3ME2xMlQ4OTNzIN88US=> NW\USFN{W0GQR1XS
NCI-H2081 NWi2OoVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXy5cIZWUUN3ME2xMlU2OTl4IN88US=> MlmyV2FPT0WU
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NCI-H2227 MnrlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnyRWdSUUN3ME2xMlYyPTZzIN88US=> NUi3[2dxW0GQR1XS
D-502MG MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHzSnlwUUN3ME2xMlY4OjFzIN88US=> NVu4PXV{W0GQR1XS
NCI-H2141 NYLjO291T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;0TmlEPTB;MT62O|MyPyEQvF2= NV\WVmZ{W0GQR1XS
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SU-DHL-1 Mom1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTDTWM2OD1zLkexNlgyKM7:TR?= Mm\pV2FPT0WU
BB30-HNC MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTFwN{K2PFUh|ryP MkPmV2FPT0WU
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JVM-3 M17I[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHu2c|lKSzVyPUGuPVM6ODRizszN NY[2PGVbW0GQR1XS
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MC-CAR M1vWbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3W4WGlEPTB;Mj6yNlk2KM7:TR?= NYTP[HZoW0GQR1XS
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HCE-T NF\UVWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;ZTWM2OD1{LkS0PFg{KM7:TR?= NVW4PHdXW0GQR1XS
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SCC-3 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\rTWM2OD1|LkGwPFQ5KM7:TR?= NFzB[IhUSU6JRWK=
RH-1 NIfUcVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTNwM{i3OFgh|ryP NH\DfpNUSU6JRWK=
NCI-H748 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K2O2lEPTB;Mz60OFI3PiEQvF2= NYL6O21iW0GQR1XS
HCC2218 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{[0XWlEPTB;Mz60Olk{PyEQvF2= NFPDNnFUSU6JRWK=
MEG-01 M1XWT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jzVmlEPTB;Mz61PVY5KM7:TR?= M3vtbXNCVkeHUh?=
NB12 M1v0fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTNwNUm4PFgh|ryP M1z4V3NCVkeHUh?=
SNB75 MoX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M12ybWlEPTB;Mz62NFExOyEQvF2= M1Xx[XNCVkeHUh?=
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COLO-320-HSR M{DiOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j5XGlEPTB;Mz63OVY{PCEQvF2= NELsPWlUSU6JRWK=
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TE-6 M4LNb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHKzNJlKSzVyPUOuPVQ2PjFizszN MWDTRW5ITVJ?
D-336MG MoT6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHDU|FvUUN3ME20MlAyOTZ4IN88US=> MmC3V2FPT0WU
NCI-H1650 M1X1O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTRwMUW3PVch|ryP M2S5[XNCVkeHUh?=
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LB647-SCLC MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPBTWM2OD12LkW2N|A5KM7:TR?= MmLiV2FPT0WU
HAL-01 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnmW49tUUN3ME20MlU4ODN2IN88US=> NHjodmZUSU6JRWK=
LP-1 NH;ZcVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrpe5hpUUN3ME20MlczOzdzIN88US=> MnOyV2FPT0WU
BC-1 NHz6[YFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTVwMEC0OFch|ryP M1;STHNCVkeHUh?=
EB-3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zIcGlEPTB;NT6wN|A1OSEQvF2= NX;oTnJ{W0GQR1XS
GT3TKB M2rPTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HHVGlEPTB;NT6xO|Y3OiEQvF2= NIHxTVhUSU6JRWK=
NCI-H209 MnTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;sTJJKSzVyPUWuNVk{PTJizszN M4KyN3NCVkeHUh?=
BT-474 M3GyPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\DPGU2UUN3ME21MlI{OTB{IN88US=> NUXjPI5oW0GQR1XS
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SIMA M3nBRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nTXmlEPTB;NT6zNFc3PSEQvF2= NYXtcFBVW0GQR1XS
RL MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonLTWM2OD13LkO3OVQyKM7:TR?= MUjTRW5ITVJ?
GCIY NY\oe4J6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;x[5BKSzVyPUWuOFczOzZizszN MorMV2FPT0WU
Calu-6 NVLxWGlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmriTWM2OD13Lk[yNkDPxE1? MorPV2FPT0WU
ALL-PO NWPjOnE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrwNoN1UUN3ME21MlY{Pzl3IN88US=> M1Tkd3NCVkeHUh?=
ARH-77 NEf0SWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3H5VWlEPTB;NT62O|A5PyEQvF2= Mmq5V2FPT0WU
A4-Fuk M1j0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\0W|k6UUN3ME22MlA5OTF6IN88US=> NH;ndmtUSU6JRWK=
NCI-H1581 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPjZXYzUUN3ME22MlI{QDR7IN88US=> MWDTRW5ITVJ?
HUTU-80 NHvXZlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTZwM{[4PVch|ryP NVy2VWJYW0GQR1XS
TGW NX:5U|hRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTZwNEW1PVMh|ryP NHnufopUSU6JRWK=
SK-N-FI NVf1WlY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nId2lEPTB;Nj60OVg2PCEQvF2= NYnQclNOW0GQR1XS
U-266 M2q0UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn70TWM2OD14LkWxPVI3KM7:TR?= Mn7xV2FPT0WU
EM-2 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Luc2lEPTB;Nj62O|A6QSEQvF2= MVrTRW5ITVJ?
NMC-G1 Ml3KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PZWGlEPTB;Nj63NVg6PSEQvF2= M{T0OnNCVkeHUh?=
KASUMI-1 MnXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3MTWM2OD14Lki0O|g4KM7:TR?= NVT0UY96W0GQR1XS
NALM-6 NX\CVY1uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M320SmlEPTB;Nj64Olc2PCEQvF2= MWTTRW5ITVJ?
OCI-AML2 MmDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jNN2lEPTB;Nz6wNFMzPiEQvF2= NH\mdYNUSU6JRWK=
SHP-77 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfLTWM2OD15LkKyOFMh|ryP MnH0V2FPT0WU
NOMO-1 NELi[YNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjHTWM2OD15LkK0NlU1KM7:TR?= MnfnV2FPT0WU
SK-N-DZ M2n4XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XtVWlEPTB;Nz63NVA5QSEQvF2= MnTlV2FPT0WU
LB1047-RCC NILuVo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HrUWlEPTB;Nz63NlI{OSEQvF2= MnT5V2FPT0WU
MZ1-PC M1HZT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH:xZmJKSzVyPUeuPFY2PThizszN Ml;SV2FPT0WU
NB10 NHrwdJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13oO2lEPTB;Nz65PVU2PiEQvF2= NWTp[28{W0GQR1XS
RL95-2 Mlr1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRThwMUC4OFIh|ryP NHvXfo5USU6JRWK=
OMC-1 M4jh[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\E[2pKSzVyPUiuOVIyQTFizszN M2W0T3NCVkeHUh?=
D-283MED NFXKNJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRThwOUG3NVkh|ryP NIT1SnZUSU6JRWK=
MC116 MlPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYe3bXl7UUN3ME24Mlk4QTRizszN NHnsNI1USU6JRWK=
SJSA-1 NUDRdHFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXi5bldbUUN3ME25MlA6ODlzIN88US=> MXTTRW5ITVJ?
JiyoyeP-2003 NH3EOVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrndHdKSzVyPUmuNlk{OTdizszN M1SxV3NCVkeHUh?=
IST-MEL1 M3fP[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTvNnhtUUN3ME25Mlc1OTl6IN88US=> NF;vbpVUSU6JRWK=
CTV-1 M4LBVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rXe2lEPTB;MUCuNFk4QSEQvF2= NGjLZZVUSU6JRWK=
NH-12 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTSTmxHUUN3ME2xNE4zPDN|IN88US=> M1zIenNCVkeHUh?=
CA46 NE\xUndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVy4dHlxUUN3ME2xNE4{PjFizszN MVzTRW5ITVJ?
NCI-SNU-1 M3TjeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7QN2dOUUN3ME2xNE41QTZ7IN88US=> M1HKT3NCVkeHUh?=
SCLC-21H MlPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfHV4VEUUN3ME2xNE43PTl4IN88US=> Mn;2V2FPT0WU
EC-GI-10 Ml65S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFyLkewN|Eh|ryP NF7RU3JUSU6JRWK=
SR NV:1VZdLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\Ee2lEPTB;MUGuNFE5PSEQvF2= MYjTRW5ITVJ?
NCI-H1648 M2rHN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPWVFdzUUN3ME2xNU4xQTZ3IN88US=> NIewVIJUSU6JRWK=
TGBC1TKB Mm[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rUWmlEPTB;MUGuOFExOiEQvF2= NHnXWplUSU6JRWK=
EW-11 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTFzLkWxPFYh|ryP MnXHV2FPT0WU
SK-MM-2 NF;ZOm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTFzLkiwOlEh|ryP NIPPSJZUSU6JRWK=
NCI-H524 M2GzVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTFzLkm4NlIh|ryP NUHBRm1KW0GQR1XS
NOS-1 M4DqNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvaTWM2OD1zMj6wN|Q2KM7:TR?= NFTiXJRUSU6JRWK=
AM-38 M37VXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGD6XXRKSzVyPUGyMlU3OzNizszN Mor4V2FPT0WU
A498 NWG0XpNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTF{LkewOlkh|ryP MlfIV2FPT0WU
KARPAS-422 MmrKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWS4SFVPUUN3ME2xNk44PDl4IN88US=> NH70VZFUSU6JRWK=
LU-139 NXzk[|Z3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPXTWM2OD1zMj65NFI3KM7:TR?= MnSwV2FPT0WU
COR-L88 M3nYV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\oXWlEPTB;MUKuPVM5OiEQvF2= MWTTRW5ITVJ?
K5 MlnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXSTWM2OD1zMj65OFYzKM7:TR?= MnniV2FPT0WU
NB13 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MomwTWM2OD1zMj65O|g{KM7:TR?= M3Pid3NCVkeHUh?=
MRK-nu-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\BcWdKSzVyPUGzMlE6PSEQvF2= MnTlV2FPT0WU
MHH-NB-11 MlnES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTF|LkK5NFIh|ryP NGrUdphUSU6JRWK=
KU812 MmTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUf1PHZNUUN3ME2xN{43OTJ2IN88US=> M{SydXNCVkeHUh?=
TE-12 NXe4bopGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTF|Lk[5PVQh|ryP NF7aPYVUSU6JRWK=
NCI-N87 NXH3PJQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTub3Y1UUN3ME2xN{44OzZizszN NVrCbYR6W0GQR1XS
EB2 NGXsVZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTF|Lki1NFUh|ryP MoHXV2FPT0WU
DB NW\YbXh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PZSWlEPTB;MUOuPVg5PSEQvF2= MVvTRW5ITVJ?
697 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXOTWM2OD1zND60OVEyKM7:TR?= M1\VdnNCVkeHUh?=
MSTO-211H MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nMNmlEPTB;MUSuO|Q1QCEQvF2= NITkcVVUSU6JRWK=
JVM-2 NXLzVJMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTF2Lke3N|Uh|ryP M2TmeXNCVkeHUh?=
COLO-824 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rPdGlEPTB;MUSuO|k6PCEQvF2= NYLsOHQ1W0GQR1XS
BC-3 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFz5WJNKSzVyPUG1MlM6QCEQvF2= MUTTRW5ITVJ?
BOKU NUfPUGpNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTF3Lkm2NFgh|ryP M{TKfHNCVkeHUh?=
GOTO M{T6O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zxbWlEPTB;MU[uPVYyPyEQvF2= NXf1XYRYW0GQR1XS
HCC2157 NF:2VItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPrfpFwUUN3ME2xO{41PzV3IN88US=> MlfYV2FPT0WU
LS-1034 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTF5Lk[4NVgh|ryP NXq3XZZkW0GQR1XS
CAL-148 NVzoO3BbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTF5Lkm3PVYh|ryP NFTYSG1USU6JRWK=
MOLT-4 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnI[Jg4UUN3ME2xPE45OjB6IN88US=> MV\TRW5ITVJ?
Daudi M3vhPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LON2lEPTB;MUiuPVI{OSEQvF2= M1LaU3NCVkeHUh?=
J-RT3-T3-5 NE\LW5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGq0R3NKSzVyPUG5MlQxPjZizszN NE\yc|FUSU6JRWK=
KMOE-2 NIPUVIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVG2TpZrUUN3ME2xPU43PDh{IN88US=> M{W1[HNCVkeHUh?=
HL-60 M4TMeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NES2XlRKSzVyPUKwMlE6PjVizszN MnzsV2FPT0WU
P31-FUJ NX:1dGhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmK5TWM2OD1{MD60O|U{KM7:TR?= NWr4eVNIW0GQR1XS
IM-9 NU\FeoFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnOSWlKSzVyPUKwMlYzOjRizszN NVvUfFhYW0GQR1XS
HDLM-2 M1jEe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELCcJZKSzVyPUKwMlg5OjFizszN MnrpV2FPT0WU
NCI-H1304 NIXnWYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFf0NnJKSzVyPUKxMlA{OTFizszN MWPTRW5ITVJ?
NCI-H345 MknCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LFOmlEPTB;MkGuNFY1OyEQvF2= NEK5fIFUSU6JRWK=
RPMI-6666 NVXoVYVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVziRmNOUUN3ME2yNU4{PjB{IN88US=> NXLD[3B5W0GQR1XS
GR-ST NFnMcZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jtd2lEPTB;MkGuOFE4KM7:TR?= NEPoU4ZUSU6JRWK=
CHP-126 MmLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWC4fVdZUUN3ME2yNU43OThzIN88US=> Mo\FV2FPT0WU
EHEB M2[3VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHlT5ZwUUN3ME2yNU43PzNzIN88US=> NWPu[4wxW0GQR1XS
CPC-N MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DDOGlEPTB;MkSuNFIxOSEQvF2= NXe2OI9MW0GQR1XS
NB1 MoDuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTJ2Lk[5N|kh|ryP NXS0fnlnW0GQR1XS
LS-123 Mn:3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3kblJKSzVyPUK0MlkxOTJizszN M1jKTnNCVkeHUh?=
ST486 M1TJd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFWyb4ZKSzVyPUK1MlEyOTNizszN MWXTRW5ITVJ?
NCI-H1963 NI\jcGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkW2TWM2OD1{Nj6wOlI{KM7:TR?= NHO1Wm1USU6JRWK=
U-87-MG MmPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\UTWM2OD1{Nj63OlQ1KM7:TR?= MmXkV2FPT0WU
COR-L279 NVTWZlhxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\ZWmlEPTB;Mk[uO|kzOiEQvF2= MmS3V2FPT0WU
LU-165 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTJ6LkC4OlEh|ryP MUjTRW5ITVJ?
COLO-800 NH7TSJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfxbXBNUUN3ME2yPE4zQTV4IN88US=> M3nXOXNCVkeHUh?=
ETK-1 NHvuSnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7MTWM2OD1{OD6zOFQ3KM7:TR?= MUDTRW5ITVJ?
LNCaP-Clone-FGC M4DNR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTncYl[UUN3ME2yPU46OjhzIN88US=> NImxWZNUSU6JRWK=
SIG-M5 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjjTWM2OD1|MD63PVUzKM7:TR?= NFryV3JUSU6JRWK=
NB6 M{j4W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDNZVY1UUN3ME2zNE46PTB|IN88US=> M1n1VnNCVkeHUh?=
NCI-H2107 MnLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;0dnBMUUN3ME2zNU4zOzN6IN88US=> NUO4N3hyW0GQR1XS
SNU-C1 MnOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1OwfmlEPTB;M{GuN|EzPiEQvF2= MUDTRW5ITVJ?
JAR MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTNzLkO3PVkh|ryP MYPTRW5ITVJ?
L-363 NUTwelliT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTN{LkKxOFQh|ryP M1XvPXNCVkeHUh?=
EW-24 M3[0[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XzeGlEPTB;M{KuN|Q4PCEQvF2= NGHqfHNUSU6JRWK=
NB69 NX32e|J4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLyWpJFUUN3ME2zN{43PDV2IN88US=> MUXTRW5ITVJ?
EW-13 NXXBO5BKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvlR2tmUUN3ME2zN{45QTZ7IN88US=> MV7TRW5ITVJ?
Ramos-2G6-4C10 Mo\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjsVW9IUUN3ME2zOE4zQTl|IN88US=> NUTWU25LW0GQR1XS
TE-11 NF[3Z2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkS1TWM2OD1|ND60PFIzKM7:TR?= MknqV2FPT0WU
L-428 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTN2Lke1N|Yh|ryP MlyyV2FPT0WU
KP-N-YN NYP1OndjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGSyS3NKSzVyPUO0MlkxPTdizszN NUHT[nFyW0GQR1XS
CGTH-W-1 NXX0[5pXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvSWWFKSzVyPUO2MlkxPDhizszN NHnwNI5USU6JRWK=
K-562 M4j0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUH6fXRYUUN3ME2zO{4xQDZ2IN88US=> Mm[2V2FPT0WU
NCI-H1299 NH;qfmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTN6LkG2NFMh|ryP MnHLV2FPT0WU
RCC10RGB NV3D[|d2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTsfIN6UUN3ME2zPE4zODh|IN88US=> M1fFcnNCVkeHUh?=
NCI-SNU-16 NHLqUIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPHTlJKSzVyPUO4MlU3PTNizszN NV;EepI{W0GQR1XS
LC-2-ad NGT3fGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETv[2pKSzVyPUO5MlQ{OzdizszN MVzTRW5ITVJ?
MHH-PREB-1 NX7BbJVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3vTWM2OD1|OT63N|g5KM7:TR?= M1zBSnNCVkeHUh?=
NCI-H64 MnPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PXVGlEPTB;NECuNFk1QCEQvF2= Mnq2V2FPT0WU
LB996-RCC MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfSNWVKSzVyPUSwMlg4OTZizszN NIXmd|FUSU6JRWK=
DEL M{DoS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTRzLkS0NFUh|ryP NITvXVNUSU6JRWK=
MLMA M3XoWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rhUmlEPTB;NEGuO|U6PSEQvF2= NV;5OppyW0GQR1XS
SBC-1 NWTCU4FLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HHPWlEPTB;NEKuNVgxPiEQvF2= MUfTRW5ITVJ?
MPP-89 NVO3W5c2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTR{LkW4O|Uh|ryP MXHTRW5ITVJ?
MV-4-11 Mm\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXztNoZRUUN3ME20Nk46ODZ5IN88US=> NWnvemhJW0GQR1XS
EoL-1- MlzNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonTTWM2OD12ND6xOVY5KM7:TR?= MkX2V2FPT0WU
CW-2 M3u5dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHucJFKSzVyPUS0Mlg{PDJizszN MmrIV2FPT0WU
HT M1TyOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXKxcpJpUUN3ME20OU44ODB5IN88US=> M1jMbXNCVkeHUh?=
SW954 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGexV3NKSzVyPUS3MlQyOjhizszN NYn2XotzW0GQR1XS
A3-KAW NFzGT2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7DSGlrUUN3ME20PU45ODZzIN88US=> NY\LXHdkW0GQR1XS
TC-YIK NYDPUpp4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml36TWM2OD13MD6wN|Y{KM7:TR?= MnfRV2FPT0WU
SW962 M{HybGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTV2LkizOVch|ryP NUD6SJZlW0GQR1XS
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NCI-H526 M4[2bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfyXFJ6UUN3ME23PE42QDd5IN88US=> MorlV2FPT0WU
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NCI-H716 NYL4RoFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTSUZpYUUN3ME24Nk46ODd3IN88US=> MVjTRW5ITVJ?
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TUR M37Ucmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TCWmlEPTB;MU[3Mlg4KM7:TR?= NWH4cpk2W0GQR1XS
NCI-H1417 NUP2ZXVRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTF6MD6zN|Eh|ryP MVTTRW5ITVJ?
IMR-5 M{DpZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTF6MT61O|Eh|ryP NVO1WVVLW0GQR1XS
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TE-441-T NEXX[GFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jzOGlEPTB;MUm5Mlc{QSEQvF2= NWXvbGRsW0GQR1XS
REH NGfKOmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3pXXJKSzVyPUKzOk43OjZizszN NVfsW2ZJW0GQR1XS
MS-1 NHjSWYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;4d2lEPTB;MkO5MlEzOSEQvF2= NWD0O2NzW0GQR1XS
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NCI-H1838 NXLlfZBJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TJZ2lEPTB;MkexMlQ2PiEQvF2= NHu0b4RUSU6JRWK=
P30-OHK M4T0d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTJ6Mz64OFch|ryP MYjTRW5ITVJ?
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... Click to View More Cell Line Experimental Data

In vivo Administration of MG-132 effectively rescues the expression levels and plasma membrane localization of dystrophin, β-dystroglycan, α-bdystroglycan, and α-sarcoglycan in skeletal muscle fibers from mdx mice, reduces muscle membrane damage, and ameliorates the histopathological signs of muscular dystrophy. [7] MG-132 treatment significantly reduces immobilization-induced skeletal muscle atrophy in mice, by downregulating the muscle-specific ubiquitin ligases atrogin-1/MAFbx and MuRF-1 mRNA. [8]

Protocol

Kinase Assay:

[1]

+ Expand

Measurement of inhibitory activities of MG-132 against 20S proteasome:

The reaction mixture for the 20S proteasome inhibitory assay contains 0.1 M Tris-acetate, pH 7.0, 20S proteasome, MG-132, and 25 μM substrate dissolved in dimethyl sulfoxide in a final volume of 1 mL. After incuba tion at 37 °C for 15 minutes, the reaction is stopped by the addition of 0.1 mL of 10% SDS and 0.9 mL of 0.1M Tris acetate, pH 9.0. The fluorescence of the reaction products is measured. To determine the IC50 against 20S proteasome, various concentrations of MG-132 are included in the assay mixture.
Cell Research:

[3]

+ Expand
  • Cell lines: KIM-2, HC11, and ES
  • Concentrations: Dissolved in DMSO, final concentrations ~25 μM
  • Incubation Time: 24 and 48 hours
  • Method:

    Cells are exposed to various concentrations of MG-132 for 24, and 48 hours. Supernatant and monolayer cells are harvested by centrifugation and fixed in 70% ethanol in PBS for staining with acridine orange. Equal volumes of cells and acridine orange (5 mg/mL in PBS) are mixed on a microscope slide and examined by fluorescence microscopy. For annexin V analysis, cells are harvested by centrifugation and stained with annexin V and propidium iodide. For cell cycle analysis, cells are rehydrated in PBS at room temperature for 10 minutes, followed by staining with propidium iodide (5 mg/mL). All samples are analyzed using a Coulter Epics XL flow cytometer.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: Male mdx (C57BL/10ScSn DMD mdx) mice
  • Formulation: Dissolved in DMSO, and diluted in PBS
  • Dosages: ~10 μg/kg/day
  • Administration: Injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 90 mg/mL (189.22 mM)
Ethanol 25 mg/mL (52.56 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+20% propylene glycol+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 475.62
Formula

C26H41N3O5

CAS No. 133407-82-6
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I want to know whether it contains EDTA or other chelating agent in this product(S2619)?

  • Answer:

    Our S2619 MG-132 doesn't contain EDTA or other chelating agent.

Proteasome Signaling Pathway Map

Proteasome Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID