MG-132

For research use only.

Catalog No.S2619 Synonyms: Z-Leu-Leu-Leu-al

1401 publications

MG-132 Chemical Structure

CAS No. 1211877-36-9

MG132 (Z-Leu-Leu-Leu-al) is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 μM and 1.2 μM for the inhibition of proteasome and calpain, respectively. MG132 activates autophagy and induces apoptosis in tumor cells.

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Selleck's MG-132 had a noticeably greater effect when compared with similar products from Sigma: This is directly due to using the (S, R, S) - (-) - 2 configuration.

P21 protein can be degraded through ubiquitylation pathway. Inhibition of proteasome activities results in the accumulation of P21 protein, therefore, the more powerful the inhibiting effect, the more P21 protein is accumulated. (testing protein: P21; Cell line: Hela; treatment condition: final concentration of MG-132 is 5μM, with a 6 hour treatment time)

Selleck's MG-132 has been cited by 1401 publications

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Choose Selective Proteasome Inhibitors

Biological Activity

Description MG132 (Z-Leu-Leu-Leu-al) is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 μM and 1.2 μM for the inhibition of proteasome and calpain, respectively. MG132 activates autophagy and induces apoptosis in tumor cells.
Targets
Proteasome [9]
(Cell-free assay)
100 nM
In vitro

MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. MG-132 also inhibits calpain with IC50 of 1.2 μM. MG-132 induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. [1] MG-132 (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. [2] MG-132 treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. [3] Unlike BzLLLCOCHO or PS-341, MG-132 treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by MG-132 than BzLLLCOCHO. [4] MG-132 (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). [5] MG-132 significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LP-1 NGq2T2pCeG:ydH;zbZMhSXO|YYm= NWrvWnAyOzByIH7N MVuyOEBp NUnieHZwTE2VTx?= MnHOTY5lfWOnczDhdI9xfG:|aYOgZpkhcW6lcnXhd4lv\yClbHXheoVlKFCDUmCgcIV3\WxiYX7kJJJm\HWlaX7nJG1kdC1zIIDyc5RmcW5ibHX2[Yw> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl3OEW4PUc,OTV7NUi1PFk9N2F-
PC3 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnOwNlAh|ryP MVm0PEBp NFTxfGxFVVOR NXzPNZlGUUN3ME2wMlYh|ryP NGjX[Gg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNk[4OlU{Pyd-MU[2PFY2Ozd:L3G+
PC12 NYHvcXF2TnWwY4Tpc44hSXO|YYm= MmfFNVAxKM7:TdMg MmPvNlQhcA>? NH22dVFFVVOR MoPJTY5pcWKrdIOgOk1QUESDLTDhcoQhUDKRMj3pcoR2[2WmIHP5eI91d3irY3n0fS=> NFX5Slg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1PFQ2PCd-MUexOVg1PTR:L3G+
LPS-stimulated RAW264.7 cells M4K3N2Z2dmO2aX;uJGF{e2G7 MYLEUXNQ NXLUdmt3UW6qaXLpeJMhVkZva3HwdIFDKESQQTDibY5lcW6p NFj2T5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SwO|I4Pyd-MUe0NFczPzd:L3G+
OCI-Ly3 MoHCSpVv[3Srb36gRZN{[Xl? NGTDVGEyOCEQvF5CpC=> NIH5NXQ1KGh? M{\DRmROW09? MWrJcoR2[2W|IHj1cYFvKEmtYYDwZWJidHCqYTDzeIFjcWyrenH0bY9vKHerdHigSWM2OCCxZjCxJO69VQ>? M2jtVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEK0NVE{Lz5zOECyOFEyOzxxYU6=
A2780 cDDP MV3BdI9xfG:|aYOgRZN{[Xl? M1yzbFI1KGh? NGDlcIRFVVOR M1LJZWlv\HWlZYOgZZBweHSxc3nzJIJ6KGmwaHnibZRqdmdiUGTFUkBl\We{YXTheIlwdg>? NHvaZW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[4OFAyQCd-MUe2PFQxOTh:L3G+
COS-7 NXq2Nmc3S3m2b4TvfIlkKEG|c3H5 NVHNNFNHOTBizszN MVfEUXNQ MUjJR|UxRDFyIN88US=> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6OEC5O{c,OThyOEiwPVc9N2F-
HuH-7 NVzuXYRqS3m2b4TvfIlkKEG|c3H5 Ml3jNVAh|ryP MVnEUXNQ NV\1T4RDUUN3MEyxNEDPxE1? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6OEC5O{c,OThyOEiwPVc9N2F-
Hepa-1c1c7 NFu2[GZHfW6ldHnvckBCe3OjeR?= M{nRO|I2KM7:TR?= NV3RSZdEPiCq NXq5cHpITE2VTx?= MX3JcoNz\WG|ZYOgUpJnOiCycn;0[YlvKGyndnXs MmCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|OUK1OFQoRjJyM{myOVQ1RC:jPh?=
IPC227F MXPDfZRwfG:6aXOgRZN{[Xl? MXqxJO69VQ>? Mk\ROFghcA>? Ml;LSG1UVw>? NWrJV41GUUN3ME2wMlA4PyEQvF2= NWrnPXkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5NlQ2OjhpPkKxPVI1PTJ6PD;hQi=>
Calu6 M3PoZmZ2dmO2aX;uJGF{e2G7 MWWxNEDPxE1? MnTvNVghcA>? NVTveZNlTE2VTx?= NIDM[HFUcWewaX\pZ4FvfGy7IHHjZ5VufWyjdHXzJIZz[XSjeHnuJJBz\WO3coPvdi=> NW[1d2drRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NFY1QDZpPkKzOVA3PDh4PD;hQi=>
IFN-gamma-induced RAW264.7 NWjJVIFPTnWwY4Tpc44hSXO|YYm= MXTEUXNQ NIPNeXNKdmirYnn0d{BvcXS{aXOgc5hq\GVicILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOC5yN{eg{txO MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ5N{K3O{c,OjJ{N{eyO|c9N2F-
HEK-293 MUfLbY5ie2ViQYPzZZk> MljQNkBidmRiMkCg{txO NX3oTlhCOiCq NFuwVWpFVVOR NHLxeFdKdmirYnn0d{BEcFRvTDDhZ5Rqfmm2eTD3bZRpKEmFNUCgc4YhQSCwTR?= MojXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEC3PVAoRjJ|NUSwO|kxRC:jPh?=
MCF10A NULSPFdYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYKwOFF4OSEQvF2= NXnVSmVkPzJiaB?= Mn71SG1UVw>? NITFZmpKSzVyPUCuNlkh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3M{KwOkc,OjRzNUOyNFY9N2F-
MCF-7 NUC4d|hTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXuxJO69VQ>? MWe3NkBp NGLoeZdFVVOR NYm2dW4{UUN3ME2wMlE{KM7:TR?= M4\DUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUWzNlA3Lz5{NEG1N|IxPjxxYU6=
HeLa NFy0cHNMcW6jc3WgRZN{[Xl? NYfnTnlnOTBizszN Mn;lNUBp MUfEUXNQ M3fueWlv\HWlZYOgVGFTWCClbHXheoFo\SCkeTDpcohq[mm2aX7nJJBzd3OnYYPvcYUh[WO2aY\peJk> NVrSTXZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNlE5OzNpPkK0N|IyQDN|PD;hQi=>
MDA-MB-231 MoH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPqTXdzOSEQvF2= M4PMSlczKGh? NFHTT3ZFVVOR NHewd4FKSzVyPUCuNVgh|ryP M4KxcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUWzNlA3Lz5{NEG1N|IxPjxxYU6=
293T MmHrR5l1d3SxeHnjJGF{e2G7 MX2xNEDPxE1? NHvGV3E4OiCq NWPPTlI4TE2VTx?= MkjUTWM2ODx{IN88US=> NYHDeVJXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlUxQDhpPkK0OlI2ODh6PD;hQi=>
293T MmnNSpVv[3Srb36gRZN{[Xl? MUexNEDPxE1? NG\oS5IzPCCq Mmn2SG1UVw>? NEHDWVZOd2SncnH0[Yx6KGmwZIXj[ZMhcGWjdD3zbI9kcw>? NHj3Z2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[yOVA5QCd-MkS2NlUxQDh:L3G+
NCI-H929 NWLjTZJiS3m2b4TvfIlkKEG|c3H5 Mn\KNUDPxE1? M3nmcFczKGh? Mn;aSG1UVw>? MVzJR|UxRTBwMUeg{txO NXmyXJdQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2NlUxQDhpPkK0OlI2ODh6PD;hQi=>
A-549 MUPDfZRwfG:6aXOgRZN{[Xl? Mn:wOFAh|ryP MlnnOFghcA>? MX;EUXNQ NIT5OFJKSzVyPEGwJO69VQ>? MmK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUe0PVYoRjJ2Nkm3OFk3RC:jPh?=
MCF-7 M2rSd2N6fG:2b4jpZ{BCe3OjeR?= MWe0NEDPxE1? MX20PEBp NV3EO5ZRTE2VTx?= NGjFVXpKSzVyPUeuN{DPxE1? NH\DZ4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
SW-480 M3KzdmN6fG:2b4jpZ{BCe3OjeR?= MVe0NEDPxE1? MnG5OFghcA>? NX7zNotPTE2VTx?= NYm0cJpvUUN3ME20JO69VQ>? MnfpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUe0PVYoRjJ2Nkm3OFk3RC:jPh?=
HL-60 M4DH[2N6fG:2b4jpZ{BCe3OjeR?= NHPv[3E1OCEQvF2= MXW0PEBp NEfaRpFFVVOR M{LUVWlEPTB:MUCg{txO NWfrfW46RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVc1QTZpPkK0Olk4PDl4PD;hQi=>
SMMC-7721 MlfGR5l1d3SxeHnjJGF{e2G7 M4nn[VQxKM7:TR?= NFLyUos1QCCq NWW5TGNDTE2VTx?= MYjJR|UxRTdwMTFOwG0> M2fod|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nkm3OFk3Lz5{NE[5O|Q6PjxxYU6=
LPS-stimulated RAW264.7 cells Mle2SpVv[3Srb36gRZN{[Xl? MXOyOUDPxE1? NGLZdolFVVOR NHjxSlJKdmirYnn0d{BvcXS{aXOgc5hq\GVicILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOC5zIN88US=> NFnaSlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
lymphoblastoid NHHOeYpHfW6ldHnvckBie3OjeR?= M4n5O2lEPTBiPTCwMlAxOiEQvF2= NHfOWo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m5Olk5Pyd-MUe5PVY6QDd:L3G+
LCL MkjPSpVv[3Srb36gZZN{[Xl? NHHQ[owyOiCq M2HtTGlEPTBiPTCwMlAxOiEQvF2= M1S3[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNki3OlA6Lz5{ME[4O|YxQTxxYU6=
HL-60 NEPMXXBHfW6ldHnvckBie3OjeR?= NWjUWmJvUUN3MDC9JFAvODZ6IN88US=> NUi0[2R7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFA3OjNpPkG1O|gxPjJ|PD;hQi=>
IPC227F Moq3SpVv[3Srb36gZZN{[Xl? NXf5WohQOSCq MlHUTWM2OCB;IECuNFc4KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ{MEm4PEc,OTZ{MkC5PFg9N2F-
RAW264.7 NXHGPGp5SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= M1LnW|E5KGh? NGTid3NKSzVyIE2gNE4xQCEQvF2= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ{ME[3O{c,OjJ4MkC2O|c9N2F-
RAW264.7 NWDrPGpTSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= M2HmclE5KGh? MVTJR|UxKD1iMD6xJO69VQ>? Mln3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MUm4O|EoRjJ|OEG5PFcyRC:jPh?=
RAW264.7 M2\0NmZ2dmO2aX;uJIF{e2G7 MlLSTWM2OCB;IECuNUDPxE1? Ml2wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OUe4PVQoRjJ2NUm3PFk1RC:jPh?=
RAW264.7 M4\y[GZ2dmO2aX;uJIF{e2G7 M3PHcFE5KGh? NGjHeZZKSzVyIE2gNE4yKM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzOUiwPUc,OjR{MUm4NFk9N2F-
RAW264.7 M1LhZmZ2dmO2aX;uJIF{e2G7 NW\abWxtOThiaB?= MX;JR|UxKD1iMD6xOUDPxE1? M{fOWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{K3OlY5Lz5{M{OyO|Y3QDxxYU6=
RAW264.7 Ml6zSpVv[3Srb36gZZN{[Xl? MofUNVghcA>? Ml7yTWM2OCB;IECuNVUh|ryP MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3N{CxPUc,OjZ5NUewNVk9N2F-
RAW264.7 NYribW83SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= NGDFbpoyQCCq M{m0OmlEPTBiPTCwMlE5KM7:TR?= NHvrcHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{KwN|I6OSd-MkeyNFMzQTF:L3G+
RAW264.7 M2X4OWFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZZN{[Xl? MUKxJIg> M2G5eWlEPTBiPTCwMlIh|ryP M2nzUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{O4NlYxLz5{OUOzPFI3ODxxYU6=
MDA-MB-231 MY\DfZRwfG:6aXPpeJkh[XO|YYm= M4LSdGlEPTBiPTCwMlI2KM7:TR?= M2DDXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUm4NFE5Lz5zOEW5PFAyQDxxYU6=
MCF7 MoTxR5l1d3SxeHnjbZR6KGG|c3H5 M4XENWlEPTBiPTCwMlMh|ryP M1Xv[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUm4NFE5Lz5zOEW5PFAyQDxxYU6=
RAW264.7 M12y[2Z2dmO2aX;uJIF{e2G7 MkXmNVghcA>? NV3acINtUUN3MDC9JFAvOzVizszN MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB2NEi0O{c,OjFyNES4OFc9N2F-
RAW264.7 NUPUPY1MSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= NFzjSFlKSzVyIE2gNE41OzlizszN M4j1PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNU[xNFYxLz5{MUW2NVA3ODxxYU6=
HEK293 NVf6XGRUTnWwY4Tpc44h[XO|YYm= Mnq2NkBp MU\JR|UxKD1iMD62JO69VQ>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV2MEe5NEc,OjN3NEC3PVA9N2F-
RAW264.7 MkPkWI95cWOrdImgZZN{[Xl? MWDJR|UxKD1iMD62N{DPxE1? M3uwbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEW4Nlk4Lz5{Mki1PFI6PzxxYU6=
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