MG-132

For research use only.

Catalog No.S2619 Synonyms: Z-Leu-Leu-Leu-al

1461 publications

MG-132 Chemical Structure

CAS No. 1211877-36-9

MG132 (Z-Leu-Leu-Leu-al) is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 μM and 1.2 μM for the inhibition of proteasome and calpain, respectively. MG132 activates autophagy and induces apoptosis in tumor cells.

Selleck's MG-132 had a noticeably greater effect when compared with similar products from Sigma: This is directly due to using the (S, R, S) - (-) - 2 configuration.

P21 protein can be degraded through ubiquitylation pathway. Inhibition of proteasome activities results in the accumulation of P21 protein, therefore, the more powerful the inhibiting effect, the more P21 protein is accumulated. (testing protein: P21; Cell line: Hela; treatment condition: final concentration of MG-132 is 5μM, with a 6 hour treatment time)

Selleck's MG-132 has been cited by 1461 publications

Purity & Quality Control

Choose Selective Proteasome Inhibitors

Biological Activity

Description MG132 (Z-Leu-Leu-Leu-al) is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 μM and 1.2 μM for the inhibition of proteasome and calpain, respectively. MG132 activates autophagy and induces apoptosis in tumor cells.
Targets
Proteasome [9]
(Cell-free assay)
100 nM
In vitro

MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. MG-132 also inhibits calpain with IC50 of 1.2 μM. MG-132 induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. [1] MG-132 (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. [2] MG-132 treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. [3] Unlike BzLLLCOCHO or PS-341, MG-132 treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by MG-132 than BzLLLCOCHO. [4] MG-132 (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). [5] MG-132 significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LP-1 NY\kWZJiSXCxcITvd4l{KEG|c3H5 NVPOTmRROzByIH7N NEDxSVAzPCCq MlX0SG1UVw>? NYTv[ZJOUW6mdXPld{BieG:ydH;zbZMh[nliaX7jdoVie2mwZzDjcIVifmWmIGDBVnAhdGW4ZXygZY5lKHKnZIXjbY5oKE2lbD2xJJBzd3SnaX6gcIV3\Wx? M1vS[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OUW4OVg6Lz5zNUm1PFU5QTxxYU6=
PC3 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\nXVIxKM7:TR?= NE\6blU1QCCq MVjEUXNQ MVLJR|UxRTBwNjFOwG0> NVriPWFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFY2OzdpPkG2Olg3PTN5PD;hQi=>
PC12 MmPGSpVv[3Srb36gRZN{[Xl? NFHWe5gyODBizszNxsA> MkPRNlQhcA>? M17MXmROW09? NVzrUFJCUW6qaXLpeJMhPi2RSFTBMUBidmRiSELPNk1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3OES1OEc,OTdzNUi0OVQ9N2F-
LPS-stimulated RAW264.7 cells NHqyc4tHfW6ldHnvckBCe3OjeR?= NIXMTHlFVVOR MX3Jcohq[mm2czDOSk1s[XCyYVKgSG5CKGKrbnTpcoc> M2rTZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEC3Nlc4Lz5zN{SwO|I4PzxxYU6=
OCI-Ly3 M3PSNGZ2dmO2aX;uJGF{e2G7 NFrOdm0yOCEQvF5CpC=> NYDxVGxYPCCq NIThNGFFVVOR NVPZOW1{UW6mdXPld{BpfW2jbjDJb4FxeGGEYXzwbIEhe3SjYnnsbZpifGmxbjD3bZRpKEWFNUCgc4YhOSEQvF2= NIj0eYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOECyOFEyOyd-MUiwNlQyOTN:L3G+
A2780 cDDP NInzOZZCeG:ydH;zbZMhSXO|YYm= MX6yOEBp MV;EUXNQ NVHkfFVpUW6mdXPld{BieG:ydH;zbZMh[nliaX7obYJqfGmwZzDQWGVPKGSnZ4Lh[IF1cW:w M4ryVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nki0NFE5Lz5zN{[4OFAyQDxxYU6=
COS-7 NYTQcVJPS3m2b4TvfIlkKEG|c3H5 NITFbHcyOCEQvF2= M3rJfmROW09? NE\tPHhKSzVyPEGwJO69VQ>? M3foNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEi4NFk4Lz5zOEC4PFA6PzxxYU6=
HuH-7 M1;MUWN6fG:2b4jpZ{BCe3OjeR?= Mo\zNVAh|ryP MYrEUXNQ NFfWdVNKSzVyPEGwJO69VQ>? NHHTPHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4PFA6Pyd-MUiwPFgxQTd:L3G+
Hepa-1c1c7 MnjMSpVv[3Srb36gRZN{[Xl? M3S0cFI2KM7:TR?= NX;ibmNwPiCq M33GdmROW09? MWTJcoNz\WG|ZYOgUpJnOiCycn;0[YlvKGyndnXs Ml6wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|OUK1OFQoRjJyM{myOVQ1RC:jPh?=
IPC227F NXLsfZhuS3m2b4TvfIlkKEG|c3H5 M1rkXVEh|ryP NFvP[FA1QCCq NIjufFJFVVOR MmTKTWM2OD1yLkC3O{DPxE1? NIL5XXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUmyOFUzQCd-MkG5NlQ2Ojh:L3G+
Calu6 MV3GeY5kfGmxbjDBd5NigQ>? M3fwUVExKM7:TR?= M4\YOFE5KGh? NVf3dWhGTE2VTx?= M3PaPXNq\26rZnnjZY51dHliYXPjeY12dGG2ZYOg[pJifGG6aX6gdJJm[3W{c3;y NUm3TVdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NFY1QDZpPkKzOVA3PDh4PD;hQi=>
IFN-gamma-induced RAW264.7 MlPoSpVv[3Srb36gRZN{[Xl? NWKzc2lNTE2VTx?= M3e5XWlvcGmkaYTzJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIIfpeIghUUN3MDDv[kAxNjB5NzFOwG0> NWL5Uo5qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO|czPzdpPkKyNlc4Ojd5PD;hQi=>
HEK-293 NEDo[4hMcW6jc3WgRZN{[Xl? NVzOb5VYOiCjbnSgNlAh|ryP NFPW[3ozKGh? NX\2XJFlTE2VTx?= MV;Jcohq[mm2czDDbHQuVCCjY4Tpeol1gSC5aYToJGlEPTBib3[gPUBvVQ>? M2ezT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUSwO|kxLz5{M{W0NFc6ODxxYU6=
MCF10A MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvRNUDPxE1? MnPEO|IhcA>? NUPtU3d1TE2VTx?= NH;l[I9KSzVyPUCuNlkh|ryP M1\5V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUWzNlA3Lz5{NEG1N|IxPjxxYU6=
MCF-7 MoPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HNXlEh|ryP MXO3NkBp M1nCN2ROW09? M4\kTWlEPTB;MD6xN{DPxE1? Ml\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUOyNFYoRjJ2MUWzNlA3RC:jPh?=
HeLa NF73enJMcW6jc3WgRZN{[Xl? NIHwdIMyOCEQvF2= M{D5fVEhcA>? MXjEUXNQ MnLqTY5lfWOnczDQRXJRKGOuZXH2ZYdmKGK7IHnubIljcXSrbnegdJJwe2Wjc3;t[UBi[3Srdnn0fS=> NEnrfIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOyNVg{Oyd-MkSzNlE5OzN:L3G+
MDA-MB-231 M13XPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[xJO69VQ>? NGTScI04OiCq MWPEUXNQ MYTJR|UxRTBwMUig{txO MnPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUOyNFYoRjJ2MUWzNlA3RC:jPh?=
293T NVG0O4dDS3m2b4TvfIlkKEG|c3H5 M4XjOlExKM7:TR?= Mnm1O|IhcA>? MmjMSG1UVw>? NHfoSlJKSzVyPEKg{txO MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ{NUC4PEc,OjR4MkWwPFg9N2F-
293T NEjWTm9HfW6ldHnvckBCe3OjeR?= M{P4NVExKM7:TR?= NWfTcXE5OjRiaB?= MYHEUXNQ M4LOVG1w\GW{YYTlcJkhcW6mdXPld{Bp\WG2LYPoc4Ns M1ftZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkK1NFg5Lz5{NE[yOVA5QDxxYU6=
NCI-H929 M3rqUWN6fG:2b4jpZ{BCe3OjeR?= M4jufFEh|ryP NFztSJo4OiCq MWDEUXNQ NWrh[4IyUUN3ME2wMlE4KM7:TR?= Ml\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4MkWwPFgoRjJ2NkK1NFg5RC:jPh?=
A-549 MYrDfZRwfG:6aXOgRZN{[Xl? MWi0NEDPxE1? M170O|Q5KGh? NX7sbmNnTE2VTx?= MnzETWM2ODxzMDFOwG0> NFPv[G89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
MCF-7 MV\DfZRwfG:6aXOgRZN{[Xl? NGW2VHI1OCEQvF2= MnXnOFghcA>? M{XxSWROW09? NXHJRo5kUUN3ME23MlMh|ryP MnK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUe0PVYoRjJ2Nkm3OFk3RC:jPh?=
SW-480 MkTZR5l1d3SxeHnjJGF{e2G7 M4PvWFQxKM7:TR?= NFXoToU1QCCq M3ThXWROW09? MXTJR|UxRTRizszN NIPGfJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
HL-60 NXjVZ5BWS3m2b4TvfIlkKEG|c3H5 M1jiPFQxKM7:TR?= NG[1NHM1QCCq M3XWOGROW09? M2PW[2lEPTB:MUCg{txO NIHTO5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
SMMC-7721 MWTDfZRwfG:6aXOgRZN{[Xl? M3jjZlQxKM7:TR?= MWG0PEBp MVLEUXNQ MYPJR|UxRTdwMTFOwG0> NEX6Noc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[5O|Q6Pid-MkS2PVc1QTZ:L3G+
LPS-stimulated RAW264.7 cells NXjidnBuTnWwY4Tpc44hSXO|YYm= M1u3XFI2KM7:TR?= MojnSG1UVw>? NFTFVFhKdmirYnn0d{BvcXS{aXOgc5hq\GVicILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOC5zIN88US=> NYDkdZdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVc1QTZpPkK0Olk4PDl4PD;hQi=>
lymphoblastoid NXO0VI9yTnWwY4Tpc44h[XO|YYm= NF\ocGxKSzVyIE2gNE4xODJizszN M3vTUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUm2PVg4Lz5zN{m5Olk5PzxxYU6=
LCL M3rnSmZ2dmO2aX;uJIF{e2G7 NHe5O|gyOiCq NFvBRnlKSzVyIE2gNE4xODJizszN M4TBfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNki3OlA6Lz5{ME[4O|YxQTxxYU6=
HL-60 M1\ucGZ2dmO2aX;uJIF{e2G7 MoTLTWM2OCB;IECuNFY5KM7:TR?= M2f4WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{iwOlI{Lz5zNUe4NFYzOzxxYU6=
IPC227F NUHmSYVSTnWwY4Tpc44h[XO|YYm= NFzjOI4yKGh? NFLjZpBKSzVyIE2gNE4xPzdizszN NYrvboJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNlA6QDhpPkG2NlIxQTh6PD;hQi=>
RAW264.7 MmXTRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDhd5NigQ>? NH7Hcm8yQCCq NFSwVo1KSzVyIE2gNE4xQCEQvF2= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ{ME[3O{c,OjJ4MkC2O|c9N2F-
RAW264.7 NXjLcplySW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= M16yNlE5KGh? NEfkZYJKSzVyIE2gNE4yKM7:TR?= NVThS41rRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NVk5PzFpPkKzPFE6QDdzPD;hQi=>
RAW264.7 MYDGeY5kfGmxbjDhd5NigQ>? MY\JR|UxKD1iMD6xJO69VQ>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV7N{i5OEc,OjR3OUe4PVQ9N2F-
RAW264.7 M4LRZWZ2dmO2aX;uJIF{e2G7 NIWyc5MyQCCq MVLJR|UxKD1iMD6xJO69VQ>? NFvkXXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxPVgxQSd-MkSyNVk5ODl:L3G+
RAW264.7 NYPIR4JOTnWwY4Tpc44h[XO|YYm= NW\FW3FMOThiaB?= Mn\GTWM2OCB;IECuNVUh|ryP M4PKOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{K3OlY5Lz5{M{OyO|Y3QDxxYU6=
RAW264.7 NWO4OG0zTnWwY4Tpc44h[XO|YYm= NV3Pd3U2OThiaB?= MXXJR|UxKD1iMD6xOUDPxE1? MknWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5NUewNVkoRjJ4N{W3NFE6RC:jPh?=
RAW264.7 Mo[zRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDhd5NigQ>? Mnm1NVghcA>? NGjYd4lKSzVyIE2gNE4yQCEQvF2= MkHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd{MEOyPVEoRjJ5MkCzNlkyRC:jPh?=
RAW264.7 Ml3hRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDhd5NigQ>? M1;jeVEhcA>? MmjYTWM2OCB;IECuNkDPxE1? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN|OEK2NEc,Ojl|M{iyOlA9N2F-
MDA-MB-231 NUjXVnZRS3m2b4TvfIlkcXS7IHHzd4F6 MkTTTWM2OCB;IECuNlUh|ryP NIrIUZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW5PFAyQCd-MUi1PVgxOTh:L3G+
MCF7 M2fuSmN6fG:2b4jpZ4l1gSCjc4PhfS=> NGD0WZFKSzVyIE2gNE4{KM7:TR?= NYjBN|ZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1PVgxOThpPkG4OVk5ODF6PD;hQi=>
RAW264.7 NWjWOXVOTnWwY4Tpc44h[XO|YYm= MojXNVghcA>? M2\WVmlEPTBiPTCwMlM2KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB2NEi0O{c,OjFyNES4OFc9N2F-
RAW264.7 NWPYfZlLSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBie3OjeR?= MoDoTWM2OCB;IECuOFM6KM7:TR?= NGjTRVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW2NVA3OCd-MkG1OlExPjB:L3G+
HEK293 NH3BR4lHfW6ldHnvckBie3OjeR?= NITQfIMzKGh? MYDJR|UxKD1iMD62JO69VQ>? NYLHWpk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFA4QTBpPkKzOVQxPzlyPD;hQi=>
RAW264.7 MVPUc5hq[2m2eTDhd5NigQ>? MV7JR|UxKD1iMD62N{DPxE1? M2HKOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEW4Nlk4Lz5{Mki1PFI6PzxxYU6=
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