SB202190 (FHPI)

Catalog No.S1077

For research use only.

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

SB202190 (FHPI) Chemical Structure

CAS No. 152121-30-7

Selleck's SB202190 (FHPI) has been cited by 129 publications

Purity & Quality Control

Choose Selective p38 MAPK Inhibitors

Other p38 MAPK Products

Biological Activity

Description SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.
Targets
Ferroptosis [11]
()
p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. [2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. [3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). [4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. [6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 NV;TfWhCTnWwY4Tpc44hSXO|YYm= MlS1NlUh|ryP NWPOdW1HOzBibXnu Mn3qSG1UV8Li NX;KTo1H[XS2ZX71ZZRmeyC2aHWgcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO|aX;uJI9nKGiERD2yJIlvKHKnc4DvcpNqfmVidH:gSGE> M3X0S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkKzNlUyLz5{NkKyN|I2OTxxYU6=
MDA-MB-231 NIPGNFZHfW6ldHnvckBCe3OjeR?= M3rkXFIh|ryP M3\JdlI1KGh? MXXs[ZN{\W6nczDDR2wzKGmwZIXjeIlwdiCkeTDUUmbPuQ>? NIT4dFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGwNFg1QCd-Mk[xNFA5PDh:L3G+
rBMSCs MYXGeY5kfGmxbjDBd5NigQ>? NVrWdJg2OTBizszN MkfiNk42KGh? MUPk[ZBz\XO|ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\iCHUlugZY5lKHB|OB?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB3M{K2Okc,OjZyNUOyOlY9N2F-
MG63 MWjGeY5kfGmxbjDBd5NigQ>? NILTTYUyOC9{MD:zNEDPxE1? MX[yOEBp NHS5TVB{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUhdGW4ZXygc4YheGixc4Doc5J6dGG2ZXSgdFM5KGmwZIXj[YQh[nliQ3TDcFIhcW5iYTDjc45k\W62cnH0bY9vKC2mZYDlcoRmdnRibXHucoVz MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl7OEOxNkc,OjV7OUizNVI9N2F-
MG63 MmLVRZBweHSxc3nzJGF{e2G7 MVyxNE8zOC9|MDFOwG0> M37vW|I1KGh? NXHCSGlCe2mpbnnmbYNidnSueTDk[YNz\WG|ZXSgeIhmKGGyb4D0c5NqeyC{YYTlJI9nKE2JNkOgbY5lfWOnZDDifUBE\EOuMh?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl7OEOxNkc,OjV7OUizNVI9N2F-
HTSMCs MlmwSpVv[3Srb36gRZN{[Xl? MXKwMlEwOS9zMDFOwG0> NYS3fHltOSCq M4jzZYlvcGmkaYTl[EBEV1KPLUKtbY5lfWOnZDDIU{0yKHC{b4TlbY4hdGW4ZXzzJIFv\CCvUl7BJIV5eHKnc4Ppc44> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl{MUS2OEc,OjV7MkG0OlQ9N2F-
MIA PaCa-2 M2TBT2Z2dmO2aX;uJGF{e2G7 MUCyNOKh|ryP M4\ZeFI1KGh? NIX5e5Vz\WS3Y3XkJIxi[3SjdHWgZYNkfW23bHH0bY9vKGmwIHPvcYJqdmG2aX;uJJdqfGhiYn;0bEAzNUSJIHHu[EBFNWGubH;z[eKh M2T1eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
MIA PaCa-2 MWrGeY5kfGmxbjDBd5NigQ>? MnfiNlDDqM7:TR?= MYqyOEBp NUXBNFBZemW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> NVi2OWJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
BxPC-3 M{S1dmZ2dmO2aX;uJGF{e2G7 MWGyNOKh|ryP Ml3mNlQhcA>? NEHFb3dz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=> NXnhSnhVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
AsPC-1 NGXzWpFHfW6ldHnvckBCe3OjeR?= NYOydlI1OjEEoN88US=> MlS4NlQhcA>? MVry[ZN2dHS|IHnuJIEhdW:mZYP0JIlvcGmkaYTpc44hd2ZiSFnGMVHPuSCycn;0[YlvKGGlY4XteYxifGmxbh?= NX7ocHhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
MIA PaCa-2 MnvwSpVv[3Srb36gRZN{[Xl? MVqyNOKh|ryP NH\DPJAzPCCq NXXnb2RI\W6qYX7j[ZMh[2ynYY\h[4Uhd2ZiUFHSVEB4cGWwIHPvcYJqdmWmIIfpeIgh\2y3Y3;z[UBidmGub3fz NV3acGJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
MIA PaCa-2 Ml\ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYmxXnV7OjEEoN88US=> MWmyJIg> MlLQd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm MoP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
BxPC-3 M3nZRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1e4eFIxyqEQvF2= M4[5ZVIhcA>? MojHd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm NHfQbno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
AsPC-1 NXjPeVVsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGr0S4kzOMLizszN NFS2bFYzKGh? NE\kSo5{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? M4XkUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
HEY M{HM[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmmyNlDDqM7:TR?= NE\xRoQzKGh? NHjJV5l{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? NVO2SXNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
OVCAR-3 NULaRVBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofTNlDDqM7:TR?= NG\rWoEzKGh? MoDwd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
SK-OV-3 NIH2ZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVWyNOKh|ryP MWKyJIg> NHntOnh{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? MoK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
MH7A  MkP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDaclM6OjRiaB?= M3LRe5JmcW6ob4Lj[ZMhfGinIHnubIljcXSxcomg[YZn\WO2czDv[kBZSU5? NV:wd3R6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OlI6PjZpPkK1PFYzQTZ4PD;hQi=>
MH7A  MV;BdI9xfG:|aYOgRZN{[Xl? NXTRNohYOjVizszN M3T3[FI1KGh? MYHy[ZZmenOnczDj[YxtKGO7Y3zlJIFzemW|dDDpcoR2[2WmIHL5JHhCViCjbnSgZ4F2e2WmIHHwc5B1d3OrczDv[kBk\WyuczD2bYEh[WO2aY\heIlwdiCxZjDKUms> M3XlZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[yPVY3Lz5{NUi2Nlk3PjxxYU6=
SCC25 M1rL[WZ2dmO2aX;uJGF{e2G7 MX6yNEDPxE1? NY\McowzOjRiaB?= NVLudo5[cW6lcnXhd4V{KGG3dH;wbIFogSCuZY\lcC=> M1;JW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAxLz5{NUizOFQxODxxYU6=
HaCaT  MYHGeY5kfGmxbjDBd5NigQ>? MkS1OUDDvU1? MWSyOEBp NF;6OmdqdmirYnn0d{BKTk5vzsOtbY5lfWOnZDDDR2wzOiCycn;keYN1cW:wIHzleoVtew>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NEO1N{c,OjV6M{SzOVM9N2F-
HaCaT  NX\3WXFwTnWwY4Tpc44hSXO|YYm= NXfKfJFTPSEEtV2= MmTXNlQhcA>? M2LPcolvcGmkaYTzJGlHVi4QsT3pcoR2[2WmIFPDUFIzKHC{b3T1Z5Rqd25ibHX2[Yx{ MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NEO1N{c,OjV6M{SzOVM9N2F-
HPAEpiCs  NXvBV|dYTnWwY4Tpc44hSXO|YYm= MYmxM|MwOTBizszN MkXtNUBp MnLWdoVlfWOnczDTNXAucW6mdXPl[EBKS0GPLUGgdJJwfGWrbjDhcoQhdVKQQTDlfJBz\XO|aX;uJIFv\CCycn;tc5RmeiCjY4Tpeol1gQ>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd|NEmwNEc,OjV5M{S5NFA9N2F-
HPAEpiCs MYPGeY5kfGmxbjDBd5NigQ>? Ml[zNU8{NzFyIN88US=> NVnDb|JTOSCq NVrie|BWcW6qaXLpeJMhWzGSLYP0bY12dGG2ZXSgRYt1KHCqb4PwbI9zgWyjdHnvckAhyqB? MmntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs MnXuSpVv[3Srb36gRZN{[Xl? M4DW[lEwOy9zMDFOwG0> M{XkOFEhcA>? M3zNUolvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u NE\6Vpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUezOFkxOCd-MkW3N|Q6ODB:L3G+
K562 MWXGeY5kfGmxbjDBd5NigQ>? NE\XNZUyOMLizszN NULCTJI4OcLiaB?= MmrUSG1UVw>? MX3pcohq[mm2czDxeYlv[WO{aX7lMYlv\HWlZXSgdFM5KE2DUFugdIhwe3Cqb4L5cIF1cW:w MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NEC0N{c,OjV4OESwOFM9N2F-
PANC-1 M3OyR2Z2dmO2aX;uJGF{e2G7 MnW3NVAh|ryP NU\NbFFPOSCq Mo[y[Y5p[W6lZYOgeIhmKGG3dH;wbIFocWNiZX\m[YN1yqB? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|MkKyNkc,OjV4M{KyNlI9N2F-
BxPC-3 M{PsXWZ2dmO2aX;uJGF{e2G7 M3H1bVExKM7:TR?= MXyxJIg> NULFTFlG\W6qYX7j[ZMhfGinIHH1eI9xcGGpaXOg[YZn\WO2wrC= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|MkKyNkc,OjV4M{KyNlI9N2F-
K562  MYnGeY5kfGmxbjDBd5NigQ>? NV:0WVJGOC5{NT2xJO69VQ>? NInEVoszPCCq MYfzeZBxemW|c3XzJJJme3[ncnH0do9tNWmwZIXj[YQhUDKDWDDwbI9{eGixconsZZRqd25? NUPOZ3puRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVk{QTJpPkK1OlE6Ozl{PD;hQi=>
THP-1 Mnn5SpVv[3Srb36gRZN{[Xl? MkCzOUDDvU1? NIezXHQzKGh? M4PT[pNq\26rZnnjZY51dHliYYT0[Y52[XSnczDz[YNz\XSrb36gc4YhUUxvMd8xJIlv\HWlZXSgZpkhOjeRSFPoc4wheGy3czDGV2wuOcLi NYW5ZnhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVg3PjFpPkK1OVk5PjZzPD;hQi=>
WB NUH2[JlGTnWwY4Tpc44hSXO|YYm= MkT5NlDjiIoQvF5CpC=> NIHLVYs{OOLCiX3pci=> NGT3d2tl\WO{ZXHz[ZMhfGinIFzQV{0hd3JiTGTBMYlv\HWlZXSgTWwuPiCjbnSgWG5HNc7zwrDwdo9lfWO2aX;u MkP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{C2PFIoRjJ3NUOwOlgzRC:jPh?=
RAW 264.7 MVjGeY5kfGmxbjDBd5NigQ>? M1;KflExyqEQvF2= NFLX[Ig{OOLCiX3pci=> NFzXb5pqdmS3Y3XzJINp[XKjY4Tldol{fGmlII\hZ5VwdGG2aX;uJI9nKE:Fc9Mg NIPIWmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NVM6QSd-MkW0OlE{QTl:L3G+
RAW 264.7 NE\ETVhHfW6ldHnvckBCe3OjeR?= NFPuVGwyOMLizszN MWGzNQKBkW2rbh?= MXHheJRmdnWjdHXzJJRp\SCnZn\lZ5R{KG:oIF;QS{BwdiCxc4Tlc4Nt[XO2IILleJJi[3Srb36= NXnFN4J[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{QTlpPkK1OFYyOzl7PD;hQi=>
HaCaT  NVvuOI56TnWwY4Tpc44hSXO|YYm= M4DZflQxyqEQvF2= Ml3UN{0zPCCq MX3EUXNQ MYLy[YR2[2W|IITo[UBi[2O3bYXsZZRqd25ib3[gXm8uOQ>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|NUS4OUc,OjV2M{W0PFU9N2F-
H9c2 M1fwWGZ2dmO2aX;uJGF{e2G7 M1fKblUxyqEQvF5CpC=> NIHrcFkyOiCq MXHy[YR2[2W|IFzETEBz\WynYYPlJIFv\CCPTWCgcI9{ew>? Ml\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NEW4NVgoRjJ3MkS1PFE5RC:jPh?=
HSCs MYXBdI9xfG:|aYOgRZN{[Xl? NFz2ZmgzPSEQvF2= NHfDcGkzPMLiaB?= MnXSd4lodmmoaXPhcpRtgSCjdITlcpVifGW|IGTHMYlv\HWlZXSgZYN1cX[jdHXkJGhUS3NiYYDvdJRwe2m| NF\vd2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NVk2OCd-MkS5OlE6PTB:L3G+
THP-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX63NkBp MVjEUXNQ MUTJR|UxRTRwN988US=> NXXJRXh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs NVntWXZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHW1T5A4OiCq M{\peGROW09? NHLufpFKSzVyMj63{txO NX\ZT5JbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs MXTGeY5kfGmxbjDBd5NigQ>? MXewMVE2KM7:TR?= M{HIOVQ5KGh? MmThSG1UVw>? MX3zeZBxemW|c3XzJGlHVi4QsTDhcoQhUVBvMUCgdJJw\HWldHnvcuKh MoXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs Ml3uSpVv[3Srb36gRZN{[Xl? MoXCNE0yPSEQvF2= M1nSN|Q5KGh? M1:4emROW09? NUSwN5FZcW6qaXLpeJMhVUmSLUHhMEBOUVBvMXKgZY5lKFKDTmTFV{Bxem:mdXP0bY9v M4\EWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG1NFg4Lz5{NEixOVA5PzxxYU6=
MDDCs NF7vXnlHfW6ldHnvckBCe3OjeR?= MUmxNEDPxE1? NWT6PZF3Oy53IHi= MkHoSG1UVw>? M3u4SoJtd2OtczDFRm9XKEeSLDDieZQhdm:2IG\TWkBIKG2nZHnheIVlKGWwdIL5JIlvfG9iaIXtZY4hVUSGQ4O= NXrrW2NqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
macrophages Mn7jSpVv[3Srb36gRZN{[Xl? M4KxV|HjiIoQvF2= M1:2V|QvPeLCiXi= NHLNU29kd22ybHX0[Yx6KGmwaHnibZR{KE2WLVnJTU1qdmS3Y3XkJIFkfGm4YYTpc44hd2ZiTl[t{tpD MkW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEi2N|MoRjJ2OEC4OlM{RC:jPh?=
PDL  NFLSXllHfW6ldHnvckBCe3OjeR?= MYSyNOKh|ryPwrC= MWqzNEBucW5? MYHEUXNQ NEDMRXl{cWewaX\pZ4FvfGy7IHnubIljcXS|IITo[UB1\W6|aXzlJIZwemOnLX3l[IlifGWmIFLNVE0zKGW6cILld5Nqd25? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV4MUC4NUc,OjR3NkGwPFE9N2F-
AGS M1v1dmZ2dmO2aX;uJGF{e2G7 MmfJOUDPxE1? M2jGfFMxKG2rbh?= NX\yVpE5e3WycILld5Nme8LiMT3pcoR2[2WmIHPhd5Bie2VvODDhcoQh[2G|cHHz[U0{KGGldHn2ZZRqd25? M3v4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUS3PFc5Lz5{NEW0O|g4QDxxYU6=
H520 NVLCOJloTnWwY4Tpc44hSXO|YYm= MVKxNOKhyrWP MlPGNUBp NX65RoN2TE2VTx?= M3nBW4Rm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO= NID5cFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N|A1PzV:L3G+
H1703 MUTGeY5kfGmxbjDBd5NigQ>? NYPyVVFoOTEEoNM1US=> NGXjWIgyKGh? M2j3O2ROW09? MVHk[YNz\WG|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy| M4PyWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
H520 Mn[2SpVv[3Srb36gRZN{[Xl? NF7q[WMyOMLiwsXN M{jC[|EzKGh? M2D3S2ROW09? M{XDeIlvcGmkaYTzJJBmdWW2cnX4[YQu\WyrY3n0[YQhVVOKMjDwdo91\WmwIIP0ZYJqdGm2eR?= NW\ENpFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703 NFz2PYZHfW6ldHnvckBCe3OjeR?= NIOwV40yOMLiwsXN NHu1WZoyOiCq MX\EUXNQ NIruXFhqdmirYnn0d{Bx\W2ndILlfIVlNWWuaXPpeIVlKE2VSEKgdJJwfGWrbjDzeIFjcWyrdIm= NHntSYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N|A1PzV:L3G+
H520 NYLLT|E6TnWwY4Tpc44hSXO|YYm= NE\qSHQyOMLiwsXN NVnURYNnPiCq MWnEUXNQ NIDKTmV{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUhdGW4ZXzzJI9nKHWkaYH1bZRqdi2lb37qeYdifGWmIF3TTFIhcW5icHXt[ZRz\XinZD30doVifGWmwrDj[YxtKGyrbnW= NWXLR494RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703 MW\GeY5kfGmxbjDBd5NigQ>? NEPFcnoyOMLiwsXN MWm2JIg> M1vncmROW09? NWnCRoF7e2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l NVfXem1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
MC3T3-E1 MonoSpVv[3Srb36gRZN{[Xl? M1TvZ|AvOy9|L{OwJO69VQ>? NVfhVXlJOSCq M4jReIF1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nctMg Ml3ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEK2PVYoRjJ2NUCyOlk3RC:jPh?=
MC3T3-E1 NHSyZ5ZHfW6ldHnvckBCe3OjeR?= MoDNN|Ah|ryP MX:xJIg> NXvO[3lz[XS2ZX71ZZRmeyCWTl[t{tEue3SrbYXsZZRm\CCyM{igUWFRUyCyaH;zdIhwenmuYYTpc44> NG[1bm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwNlY6Pid-MkS1NFI3QTZ:L3G+
HUVECs  MorvSpVv[3Srb36gRZN{[Xl? Mmj1NVDDqML3TR?= MlvrNUBp M2no[olvcGmkaYTzJHRPTi4QsT3pcoR2[2WmIFPYR2wyKHC{b3T1Z5Rqd25? NUnaT4RlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PFc6PjRpPkK0OFg4QTZ2PD;hQi=>
AGS  NYjBPVF1TnWwY4Tpc44hSXO|YYm= NXPRfm9yOTEEoNM1US=> MoHTN|DDqG2rbh?= Mm\wbY5pcWKrdIOgTWwuOc7{LXnu[JVk\WRiYXP0bZZifGmxbjDv[kBxOzh? NXTi[GQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45 NELHZY5HfW6ldHnvckBCe3OjeR?= NWjOeWRyOTEEoNM1US=> MnPhN|DDqG2rbh?= M2P6dYlvcGmkaYTzJGlNNTIQsj3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6 MoTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
AGS  MXPGeY5kfGmxbjDBd5NigQ>? MlfrNVDDqML3TR?= M1XyXVMxyqCvaX6= M2PJeYF1fGWwdXH0[ZMhUUxvMd8yMYlv\HWlZXSgS2Eh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9v NYj1bnZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45 Mm\lSpVv[3Srb36gRZN{[Xl? NXvHboNpOTEEoNM1US=> NVjPPVJMOzEEoH3pci=> NHPm[XpifHSnboXheIV{KEmOLUJOtk1qdmS3Y3XkJGdCKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdg>? M1vvVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEe5OlgyLz5{NES3PVY5OTxxYU6=
AGS  NGq1fotHfW6ldHnvckBCe3OjeR?= M2\DblExyqEEtV2= NUXzS4VLOzEEoH3pci=> Mo\Jd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25? MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
MKN-45 NHfLWndHfW6ldHnvckBCe3OjeR?= MUixNOKhyrWP M{TtR|MxyqCvaX6= Ml7Qd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25? NYfqWXVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
DCs MoXOSpVv[3Srb36gRZN{[Xl? M2jzTVIxyqEQvF2= NIn3UFEyyqCq NES2T3Rl\WO{ZXHz[ZMhUUxvMUKgdJJw\HWldHnvci=> NWjVZm5mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|Q3OzZpPkK0OFM1PjN4PD;hQi=>
HUVEC  NWTPdXZ7TnWwY4Tpc44hSXO|YYm= MXiyNEDPxG4EoB?= NVPrXIV3PSCq MYLEUXNQ NG\TZ4dz\WS3Y3XzJIN6fG:taX7lJIV5eHKnc4Ppc44hdGW4ZXzzJIlvKGFiY3;uZ4VvfHKjdHnvck1l\XCnbnTlcpQhdWGwbnXy NHi1dHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4PVA3Oid-MkSxPFkxPjJ:L3G+
A 549 MonFSpVv[3Srb36gRZN{[Xl? NYThVllFPTBizszN NIjOWoQyKGh? NH3sUFZl\WO{ZXHz[ZMhfGinIHzleoVtKG:oIFnMMVjDqA>? NV;CdI8{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|k3QDhpPkK0NVc6Pjh6PD;hQi=>
H520  MlT0SpVv[3Srb36gRZN{[Xl? MVi1M|ExKM7:TR?= M4HZRVEhcA>? Mk\hSG1UVw>? NGnHdI1l\WO{ZXHz[ZMhVVOKMjDwdo91\WmwIHHzJJdmdGxiYYOgcXJPSSCuZY\lcJMhcW5iZ3XmbZRqdmmkLXX4dI9{\WRiY3XscC=> NUnITJhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|g6ODNpPkK0NVM5QTB|PD;hQi=>
H1703  NGP3WZpHfW6ldHnvckBCe3OjeR?= NXLXcmlJPS9zMDFOwG0> NXH2XI5xOSCq MoixSG1UVw>? NVjnUpBm\GWlcnXhd4V{KE2VSEKgdJJwfGWrbjDhd{B4\WyuIHHzJI1TVkFibHX2[Yx{KGmwIHfl[ol1cW6rYj3lfJBwe2WmIHPlcIw> MmD5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
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H1703  MUTGeY5kfGmxbjDBd5NigQ>? MYGxNOKhyrWP NW\iZ4N6OTJiaB?= NEPCVlNFVVOR NEmwNIFl\WO{ZXHz[ZMhVVOKMjDtVm5CKGGwZDDwdo91\WmwIIP0ZYJqdGm2eTDpckBo\W[rdHnubYIufHKnYYTl[EBPW0OOQzDj[Yxtew>? NWfVc49DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|g6ODNpPkK0NVM5QTB|PD;hQi=>
MCF-7  M3S5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV2xNOKh|ryP MnjyNlTDqGh? NFK4TItqdmirYnn0d{B1cGViQ2KxNFgucW6mdXPl[EBk\WyuIHTlZZRp M2jXelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK4PFU{Lz5{NEGyPFg2OzxxYU6=
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podocytes MXfGeY5kfGmxbjDBd5NigQ>? M1nCcVExyqEQvF2= MXmxJIg> NF;0U4JqdmirYnn0d{BVT0cQskGtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB|OF3BVGsh[W6mIFXyb|EwOsLi M2q0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEO2NlEzLz5{NECzOlIyOjxxYU6=
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HAPI MWDGeY5kfGmxbjDBd5NigQ>? M{HH[FExNzJyL{SwxsDPxE1? Mm\CNUBp M1fBd4lvcGmkaYTzJHRETERvaX7keYNm\CCyM{ivTm5MKE2DUFugdIhwe3Cqb4L5cIF1cW:w NGfNcmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PVEzOCd-MkO5OlkyOjB:L3G+
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HepG2 MkHsSpVv[3Srb36gRZN{[Xl? M{LGbVM2OCCwTR?= MXmyOEBp MmjabY5pcWKrdIOgeIhmKGSnZ4XlcIlvNWmwZIXj[YQh[WO2aY\heIlwdiCxZjDwN|hOSVCN NEeyV|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzN|E6QCd-MkO5N|MyQTh:L3G+
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BEL7404 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDCclkxNTVyIN88US=> MXm0PEBp NUD1U4RRcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> M3vwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC3OVA5Lz5{M{iwO|UxQDxxYU6=
HL7702 M2PTOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo[0NE02OCEQvF2= NVPIXYRVPDhiaB?= NYXaTmgxcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NYmxTHRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NFc2ODhpPkKzPFA4PTB6PD;hQi=>
HepG2 M3TjfWZ2dmO2aX;uJGF{e2G7 MWiwMVUxKM7:TR?= MnHBNlQhcA>? M2\jbolvcGmkaYTzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZicEO4JIRwf26|dILlZY0heHKxdHXpcpMhVUGSS1HQT|ItKEGWRkKsJG1UUzFiYX7kJGhUWDJ5IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ NGXrRnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO|UxQCd-MkO4NFc2ODh:L3G+
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A549 M1W3dGZ2dmO2aX;uJGF{e2G7 MYCxNOKh|ryPwrC= MXewMVMxKG2rbh?= NITibndqdmirYnn0d{BCXFEQs2OgbY5lfWOnZDDwOFIweDR2IF3BVGsh[W6mIICzPEBOSVCNIIDoc5NxcG:{eXzheIlwdg>? NXzBXos5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549 M3nwS2Z2dmO2aX;uJGF{e2G7 NGLCTGEyOMLizszNxsA> NFz1U2MyKGh? MUnpcohq[mm2czDBWHDPu1NiaX7keYNm\CCQRj5OvmIheDZ3IIP1ZpVvcXRicHjvd5Bpd3K7bHH0bY9vKGGwZDDOSk3PwkJicILvcY91\XJiYXP0bZZqfHl? M1nZTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkiwOlc1Lz5{M{[4NFY4PDxxYU6=
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H1650  MnPjSpVv[3Srb36gRZN{[Xl? MVyxNOKh|ryPwrC= NX7XUGhTOSCq MYfEUXNQ MnzD[IVkemWjc3XzJIJwfGhicILveIVqdiCjbnSgcXJPSSCuZY\lcJMhd2ZiRWLDR|EhcW5icHHjcIl1[XinbD3lfJBwe2WmwrDj[Yxtew>? NVXpW3U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNlg3QTZpPkKzNlI5Pjl4PD;hQi=>
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medulloblastoma cells MnqwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1TOdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUA{NjByNjFOwG0v MoqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N|EoRjF5NEG3OlMyRC:jPh?=
neural precursor cells NX\QbVRxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NXOyVWxOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDu[ZVz[WxicILlZ5Vze2:{IHPlcIx{KGK7IF3UWEBie3OjeTygSWM2OCB;IEiuNFY{KM7:TT6= NFXKeYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxO|Y{OSd-MUe0NVc3OzF:L3G+
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NB1643 NYXkPGdMeUiWUzDhd5NigQ>? MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 M1nhNJFJXFNiYYPzZZk> M3LncZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{ M37v[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 MonmdWhVWyCjc4PhfS=> MXzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDMRW4uPSClZXzsdy=> MkPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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A673 NHzsZ49yUFSVIHHzd4F6 NIrkb4tyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? MnnPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 NF;rRphyUFSVIHHzd4F6 NGGwbo1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1|NzDj[Yxtew>? NWLlN|hMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) NXXyPY1ReUiWUzDhd5NigQ>? MlvwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUWchPjNiKE[tWGchWiliY3XscJM> NWHycIZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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OHS-50 Mmj0dWhVWyCjc4PhfS=> MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJM> NV6xTFFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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NB1643 M{DvU5FJXFNiYYPzZZk> MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORlE3PDNiY3XscJM> MoT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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TC32 Mn3LdWhVWyCjc4PhfS=> M4K0dpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NGLhS449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Assay
Methods Test Index PMID
Western blot mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2 ; pJNK1 / pJNK2 / JNK1 / JNK2 26844273 18222647
Immunofluorescence p-p38 / MMP9 24479681
Growth inhibition assay Cell viability 26844273
In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. [5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. [8]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro kinase assays:

    The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-33P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-33P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.

Cell Research:

[2]

  • Cell lines: Jurkat, and HeLa
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.

  • (Only for Reference)
Animal Research:

[5]

  • Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG
  • Dosages: 12.5 μg
  • Administration: Administered via i.d.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 66 mg/mL
(199.19 mM)
Ethanol 12 mg/mL
(36.21 mM)
Water Insoluble

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%H2O
For best results, use promptly after mixing.

8 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.34
Formula

C20H14N3OF

CAS No. 152121-30-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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