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SB202190 (FHPI)

Name: SB202190 (FHPI)
Brand: Selleck Chemicals
SKU: S1077
Rating: 5 (129 reviews)

Catalog No.S1077

For research use only.

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

CAS No. 152121-30-7

Selleck's SB202190 (FHPI) has been cited by 129 publications

Purity & Quality Control

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p38 MAPK Signaling Pathway Map

Biological Activity

Description

Targets

Ferroptosis ^[11] ()	p38α ^[1] (Cell-free assay)	p38β ^[1] (Cell-free assay)
	50 nM	100 nM

In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. ^[2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. ^[3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). ^[4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. ^[6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. ^[7]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HCT-116	NV;TfWhCTnWwY4Tpc44hSXO\|YYm=	MlS1NlUh\|ryP	NWPOdW1HOzBibXnu	Mn3qSG1UV8Li	NX;KTo1H[XS2ZX71ZZRmeyC2aHWgcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO\|aX;uJI9nKGiERD2yJIlvKHKnc4DvcpNqfmVidH:gSGE>	M3X0S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkKzNlUyLz5{NkKyN\|I2OTxxYU6=
MDA-MB-231	NIPGNFZHfW6ldHnvckBCe3OjeR?=	M3rkXFIh\|ryP	M3\JdlI1KGh?		MXXs[ZN{\W6nczDDR2wzKGmwZIXjeIlwdiCkeTDUUmbPuQ>?	NIT4dFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGwNFg1QCd-Mk[xNFA5PDh:L3G+
rBMSCs	MYXGeY5kfGmxbjDBd5NigQ>?	NVrWdJg2OTBizszN	MkfiNk42KGh?		MUPk[ZBz\XO\|ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\iCHUlugZY5lKHB\|OB?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB3M{K2Okc,OjZyNUOyOlY9N2F-
MG63	MWjGeY5kfGmxbjDBd5NigQ>?	NILTTYUyOC9{MD:zNEDPxE1?	MX[yOEBp		NHS5TVB{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUhdGW4ZXygc4YheGixc4Doc5J6dGG2ZXSgdFM5KGmwZIXj[YQh[nliQ3TDcFIhcW5iYTDjc45k\W62cnH0bY9vKC2mZYDlcoRmdnRibXHucoVz	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl7OEOxNkc,OjV7OUizNVI9N2F-
MG63	MmLVRZBweHSxc3nzJGF{e2G7	MVyxNE8zOC9\|MDFOwG0>	M37vW\|I1KGh?		NXHCSGlCe2mpbnnmbYNidnSueTDk[YNz\WG\|ZXSgeIhmKGGyb4D0c5NqeyC{YYTlJI9nKE2JNkOgbY5lfWOnZDDifUBE\EOuMh?=	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl7OEOxNkc,OjV7OUizNVI9N2F-
HTSMCs	MlmwSpVv[3Srb36gRZN{[Xl?	MXKwMlEwOS9zMDFOwG0>	NYS3fHltOSCq		M4jzZYlvcGmkaYTl[EBEV1KPLUKtbY5lfWOnZDDIU{0yKHC{b4TlbY4hdGW4ZXzzJIFv\CCvUl7BJIV5eHKnc4Ppc44>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl{MUS2OEc,OjV7MkG0OlQ9N2F-
MIA PaCa-2	M2TBT2Z2dmO2aX;uJGF{e2G7	MUCyNOKh\|ryP	M4\ZeFI1KGh?		NIX5e5Vz\WS3Y3XkJIxi[3SjdHWgZYNkfW23bHH0bY9vKGmwIHPvcYJqdmG2aX;uJJdqfGhiYn;0bEAzNUSJIHHu[EBFNWGubH;z[eKh	M2T1eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
MIA PaCa-2	MWrGeY5kfGmxbjDBd5NigQ>?	MnfiNlDDqM7:TR?=	MYqyOEBp		NUXBNFBZemW\|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44>	NVi2OWJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
BxPC-3	M{S1dmZ2dmO2aX;uJGF{e2G7	MWGyNOKh\|ryP	Ml3mNlQhcA>?		NEHFb3dz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=>	NXnhSnhVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
AsPC-1	NGXzWpFHfW6ldHnvckBCe3OjeR?=	NYOydlI1OjEEoN88US=>	MlS4NlQhcA>?		MVry[ZN2dHS\|IHnuJIEhdW:mZYP0JIlvcGmkaYTpc44hd2ZiSFnGMVHPuSCycn;0[YlvKGGlY4XteYxifGmxbh?=	NX7ocHhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
MIA PaCa-2	MnvwSpVv[3Srb36gRZN{[Xl?	MVqyNOKh\|ryP	NH\DPJAzPCCq		NXXnb2RI\W6qYX7j[ZMh[2ynYY\h[4Uhd2ZiUFHSVEB4cGWwIHPvcYJqdmWmIIfpeIgh\2y3Y3;z[UBidmGub3fz	NV3acGJORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
MIA PaCa-2	Ml\ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYmxXnV7OjEEoN88US=>	MWmyJIg>		MlLQd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm	MoP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
BxPC-3	M3nZRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1e4eFIxyqEQvF2=	M4[5ZVIhcA>?		MojHd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm	NHfQbno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
AsPC-1	NXjPeVVsT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGr0S4kzOMLizszN	NFS2bFYzKGh?		NE\kSo5{\W6\|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V?	M4XkUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
HEY	M{HM[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmmyNlDDqM7:TR?=	NE\xRoQzKGh?		NHjJV5l{\W6\|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V?	NVO2SXNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
OVCAR-3	NULaRVBuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MofTNlDDqM7:TR?=	NG\rWoEzKGh?		MoDwd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
SK-OV-3	NIH2ZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVWyNOKh\|ryP	MWKyJIg>		NHntOnh{\W6\|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V?	MoK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
MH7A	MkP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NUDaclM6OjRiaB?=		M3LRe5JmcW6ob4Lj[ZMhfGinIHnubIljcXSxcomg[YZn\WO2czDv[kBZSU5?	NV:wd3R6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4OlI6PjZpPkK1PFYzQTZ4PD;hQi=>
MH7A	MV;BdI9xfG:\|aYOgRZN{[Xl?	NXTRNohYOjVizszN	M3T3[FI1KGh?		MYHy[ZZmenOnczDj[YxtKGO7Y3zlJIFzemW\|dDDpcoR2[2WmIHL5JHhCViCjbnSgZ4F2e2WmIHHwc5B1d3OrczDv[kBk\WyuczD2bYEh[WO2aY\heIlwdiCxZjDKUms>	M3XlZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[yPVY3Lz5{NUi2Nlk3PjxxYU6=
SCC25	M1rL[WZ2dmO2aX;uJGF{e2G7	MX6yNEDPxE1?	NY\McowzOjRiaB?=		NVLudo5[cW6lcnXhd4V{KGG3dH;wbIFogSCuZY\lcC=>	M1;JW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAxLz5{NUizOFQxODxxYU6=
HaCaT	MYHGeY5kfGmxbjDBd5NigQ>?	MkS1OUDDvU1?	MWSyOEBp		NF;6OmdqdmirYnn0d{BKTk5vzsOtbY5lfWOnZDDDR2wzOiCycn;keYN1cW:wIHzleoVtew>?	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh\|NEO1N{c,OjV6M{SzOVM9N2F-
HaCaT	NX\3WXFwTnWwY4Tpc44hSXO\|YYm=	NXfKfJFTPSEEtV2=	MmTXNlQhcA>?		M2LPcolvcGmkaYTzJGlHVi4QsT3pcoR2[2WmIFPDUFIzKHC{b3T1Z5Rqd25ibHX2[Yx{	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh\|NEO1N{c,OjV6M{SzOVM9N2F-
HPAEpiCs	NXvBV\|dYTnWwY4Tpc44hSXO\|YYm=	MYmxM\|MwOTBizszN	MkXtNUBp		MnLWdoVlfWOnczDTNXAucW6mdXPl[EBKS0GPLUGgdJJwfGWrbjDhcoQhdVKQQTDlfJBz\XO\|aX;uJIFv\CCycn;tc5RmeiCjY4Tpeol1gQ>?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd\|NEmwNEc,OjV5M{S5NFA9N2F-
HPAEpiCs	MYPGeY5kfGmxbjDBd5NigQ>?	Ml[zNU8{NzFyIN88US=>	NVnDb\|JTOSCq		NVrie\|BWcW6qaXLpeJMhWzGSLYP0bY12dGG2ZXSgRYt1KHCqb4PwbI9zgWyjdHnvckAhyqB?	MmntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs	MnXuSpVv[3Srb36gRZN{[Xl?	M4DW[lEwOy9zMDFOwG0>	M{XkOFEhcA>?		M3zNUolvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u	NE\6Vpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUezOFkxOCd-MkW3N\|Q6ODB:L3G+
K562	MWXGeY5kfGmxbjDBd5NigQ>?	NE\XNZUyOMLizszN	NULCTJI4OcLiaB?=	MmrUSG1UVw>?	MX3pcohq[mm2czDxeYlv[WO{aX7lMYlv\HWlZXSgdFM5KE2DUFugdIhwe3Cqb4L5cIF1cW:w	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NEC0N{c,OjV4OESwOFM9N2F-
PANC-1	M3OyR2Z2dmO2aX;uJGF{e2G7	MnW3NVAh\|ryP	NU\NbFFPOSCq		Mo[y[Y5p[W6lZYOgeIhmKGG3dH;wbIFocWNiZX\m[YN1yqB?	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ\|MkKyNkc,OjV4M{KyNlI9N2F-
BxPC-3	M{PsXWZ2dmO2aX;uJGF{e2G7	M3H1bVExKM7:TR?=	MXyxJIg>		NULFTFlG\W6qYX7j[ZMhfGinIHH1eI9xcGGpaXOg[YZn\WO2wrC=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ\|MkKyNkc,OjV4M{KyNlI9N2F-
K562	MYnGeY5kfGmxbjDBd5NigQ>?	NV:0WVJGOC5{NT2xJO69VQ>?	NInEVoszPCCq		MYfzeZBxemW\|c3XzJJJme3[ncnH0do9tNWmwZIXj[YQhUDKDWDDwbI9{eGixconsZZRqd25?	NUPOZ3puRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVk{QTJpPkK1OlE6Ozl{PD;hQi=>
THP-1	Mnn5SpVv[3Srb36gRZN{[Xl?	MkCzOUDDvU1?	NIezXHQzKGh?		M4PT[pNq\26rZnnjZY51dHliYYT0[Y52[XSnczDz[YNz\XSrb36gc4YhUUxvMd8xJIlv\HWlZXSgZpkhOjeRSFPoc4wheGy3czDGV2wuOcLi	NYW5ZnhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVg3PjFpPkK1OVk5PjZzPD;hQi=>
WB	NUH2[JlGTnWwY4Tpc44hSXO\|YYm=	MkT5NlDjiIoQvF5CpC=>	NIHLVYs{OOLCiX3pci=>		NGT3d2tl\WO{ZXHz[ZMhfGinIFzQV{0hd3JiTGTBMYlv\HWlZXSgTWwuPiCjbnSgWG5HNc7zwrDwdo9lfWO2aX;u	MkP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{C2PFIoRjJ3NUOwOlgzRC:jPh?=
RAW 264.7	MVjGeY5kfGmxbjDBd5NigQ>?	M1;KflExyqEQvF2=	NFLX[Ig{OOLCiX3pci=>		NFzXb5pqdmS3Y3XzJINp[XKjY4Tldol{fGmlII\hZ5VwdGG2aX;uJI9nKE:Fc9Mg	NIPIWmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NVM6QSd-MkW0OlE{QTl:L3G+
RAW 264.7	NE\ETVhHfW6ldHnvckBCe3OjeR?=	NFPuVGwyOMLizszN	MWGzNQKBkW2rbh?=		MXHheJRmdnWjdHXzJJRp\SCnZn\lZ5R{KG:oIF;QS{BwdiCxc4Tlc4Nt[XO2IILleJJi[3Srb36=	NXnFN4J[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{QTlpPkK1OFYyOzl7PD;hQi=>
HaCaT	NVvuOI56TnWwY4Tpc44hSXO\|YYm=	M4DZflQxyqEQvF2=	Ml3UN{0zPCCq	MX3EUXNQ	MYLy[YR2[2W\|IITo[UBi[2O3bYXsZZRqd25ib3[gXm8uOQ>?	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NUS4OUc,OjV2M{W0PFU9N2F-
H9c2	M1fwWGZ2dmO2aX;uJGF{e2G7	M1fKblUxyqEQvF5CpC=>	NIHrcFkyOiCq		MXHy[YR2[2W\|IFzETEBz\WynYYPlJIFv\CCPTWCgcI9{ew>?	Ml\4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{NEW4NVgoRjJ3MkS1PFE5RC:jPh?=
HSCs	MYXBdI9xfG:\|aYOgRZN{[Xl?	NFz2ZmgzPSEQvF2=	NHfDcGkzPMLiaB?=		MnXSd4lodmmoaXPhcpRtgSCjdITlcpVifGW\|IGTHMYlv\HWlZXSgZYN1cX[jdHXkJGhUS3NiYYDvdJRwe2m\|	NF\vd2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NVk2OCd-MkS5OlE6PTB:L3G+
THP-1	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MX63NkBp	MVjEUXNQ	MUTJR\|UxRTRwN988US=>	NXXJRXh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs	NVntWXZyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NHW1T5A4OiCq	M{\peGROW09?	NHLufpFKSzVyMj63{txO	NX\ZT5JbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs	MXTGeY5kfGmxbjDBd5NigQ>?	MXewMVE2KM7:TR?=	M{HIOVQ5KGh?	MmThSG1UVw>?	MX3zeZBxemW\|c3XzJGlHVi4QsTDhcoQhUVBvMUCgdJJw\HWldHnvcuKh	MoXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs	Ml3uSpVv[3Srb36gRZN{[Xl?	MoXCNE0yPSEQvF2=	M1nSN\|Q5KGh?	M1:4emROW09?	NUSwN5FZcW6qaXLpeJMhVUmSLUHhMEBOUVBvMXKgZY5lKFKDTmTFV{Bxem:mdXP0bY9v	M4\EWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEG1NFg4Lz5{NEixOVA5PzxxYU6=
MDDCs	NF7vXnlHfW6ldHnvckBCe3OjeR?=	MUmxNEDPxE1?	NWT6PZF3Oy53IHi=	MkHoSG1UVw>?	M3u4SoJtd2OtczDFRm9XKEeSLDDieZQhdm:2IG\TWkBIKG2nZHnheIVlKGWwdIL5JIlvfG9iaIXtZY4hVUSGQ4O=	NXrrW2NqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
macrophages	Mn7jSpVv[3Srb36gRZN{[Xl?	M4KxV\|HjiIoQvF2=	M1:2V\|QvPeLCiXi=		NHLNU29kd22ybHX0[Yx6KGmwaHnibZR{KE2WLVnJTU1qdmS3Y3XkJIFkfGm4YYTpc44hd2ZiTl[t{tpD	MkW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEi2N\|MoRjJ2OEC4OlM{RC:jPh?=
PDL	NFLSXllHfW6ldHnvckBCe3OjeR?=	MYSyNOKh\|ryPwrC=	MWqzNEBucW5?	MYHEUXNQ	NEDMRXl{cWewaX\pZ4FvfGy7IHnubIljcXS\|IITo[UB1\W6\|aXzlJIZwemOnLX3l[IlifGWmIFLNVE0zKGW6cILld5Nqd25?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV4MUC4NUc,OjR3NkGwPFE9N2F-
AGS	M1v1dmZ2dmO2aX;uJGF{e2G7	MmfJOUDPxE1?	M2jGfFMxKG2rbh?=		NX\yVpE5e3WycILld5Nme8LiMT3pcoR2[2WmIHPhd5Bie2VvODDhcoQh[2G\|cHHz[U0{KGGldHn2ZZRqd25?	M3v4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUS3PFc5Lz5{NEW0O\|g4QDxxYU6=
H520	NVLCOJloTnWwY4Tpc44hSXO\|YYm=	MVKxNOKhyrWP	MlPGNUBp	NX65RoN2TE2VTx?=	M3nBW4Rm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO=	NID5cFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N\|A1PzV:L3G+
H1703	MUTGeY5kfGmxbjDBd5NigQ>?	NYPyVVFoOTEEoNM1US=>	NGXjWIgyKGh?	M2j3O2ROW09?	MVHk[YNz\WG\|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy\|	M4PyWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
H520	Mn[2SpVv[3Srb36gRZN{[Xl?	NF7q[WMyOMLiwsXN	M{jC[\|EzKGh?	M2D3S2ROW09?	M{XDeIlvcGmkaYTzJJBmdWW2cnX4[YQu\WyrY3n0[YQhVVOKMjDwdo91\WmwIIP0ZYJqdGm2eR?=	NW\ENpFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N\|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703	NFz2PYZHfW6ldHnvckBCe3OjeR?=	NIOwV40yOMLiwsXN	NHu1WZoyOiCq	MX\EUXNQ	NIruXFhqdmirYnn0d{Bx\W2ndILlfIVlNWWuaXPpeIVlKE2VSEKgdJJwfGWrbjDzeIFjcWyrdIm=	NHntSYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N\|A1PzV:L3G+
H520	NYLLT\|E6TnWwY4Tpc44hSXO\|YYm=	NE\qSHQyOMLiwsXN	NVnURYNnPiCq	MWnEUXNQ	NIDKTmV{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUhdGW4ZXzzJI9nKHWkaYH1bZRqdi2lb37qeYdifGWmIF3TTFIhcW5icHXt[ZRz\XinZD30doVifGWmwrDj[YxtKGyrbnW=	NWXLR494RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N\|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703	MW\GeY5kfGmxbjDBd5NigQ>?	NEPFcnoyOMLiwsXN	MWm2JIg>	M1vncmROW09?	NWnCRoF7e2mpbnnmbYNidnSueTDpcoNz\WG\|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l	NVfXem1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N\|A1PzVpPkK0OVMxPDd3PD;hQi=>
MC3T3-E1	MonoSpVv[3Srb36gRZN{[Xl?	M1TvZ\|AvOy9\|L{OwJO69VQ>?	NVfhVXlJOSCq		M4jReIF1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nctMg	Ml3ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEK2PVYoRjJ2NUCyOlk3RC:jPh?=
MC3T3-E1	NHSyZ5ZHfW6ldHnvckBCe3OjeR?=	MoDNN\|Ah\|ryP	MX:xJIg>		NXvO[3lz[XS2ZX71ZZRmeyCWTl[t{tEue3SrbYXsZZRm\CCyM{igUWFRUyCyaH;zdIhwenmuYYTpc44>	NG[1bm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwNlY6Pid-MkS1NFI3QTZ:L3G+
HUVECs	MorvSpVv[3Srb36gRZN{[Xl?	Mmj1NVDDqML3TR?=	MlvrNUBp		M2no[olvcGmkaYTzJHRPTi4QsT3pcoR2[2WmIFPYR2wyKHC{b3T1Z5Rqd25?	NUnaT4RlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PFc6PjRpPkK0OFg4QTZ2PD;hQi=>
AGS	NYjBPVF1TnWwY4Tpc44hSXO\|YYm=	NXPRfm9yOTEEoNM1US=>	MoHTN\|DDqG2rbh?=		Mm\wbY5pcWKrdIOgTWwuOc7{LXnu[JVk\WRiYXP0bZZifGmxbjDv[kBxOzh?	NXTi[GQ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45	NELHZY5HfW6ldHnvckBCe3OjeR?=	NWjOeWRyOTEEoNM1US=>	MnPhN\|DDqG2rbh?=		M2P6dYlvcGmkaYTzJGlNNTIQsj3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6	MoTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
AGS	MXPGeY5kfGmxbjDBd5NigQ>?	MlfrNVDDqML3TR?=	M1XyXVMxyqCvaX6=		M2PJeYF1fGWwdXH0[ZMhUUxvMd8yMYlv\HWlZXSgS2Eh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9v	NYj1bnZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45	Mm\lSpVv[3Srb36gRZN{[Xl?	NXvHboNpOTEEoNM1US=>	NVjPPVJMOzEEoH3pci=>		NHPm[XpifHSnboXheIV{KEmOLUJOtk1qdmS3Y3XkJGdCKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdg>?	M1vvVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEe5OlgyLz5{NES3PVY5OTxxYU6=
AGS	NGq1fotHfW6ldHnvckBCe3OjeR?=	M2\DblExyqEEtV2=	NUXzS4VLOzEEoH3pci=>		Mo\Jd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25?	MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
MKN-45	NHfLWndHfW6ldHnvckBCe3OjeR?=	MUixNOKhyrWP	M{TtR\|MxyqCvaX6=		Ml7Qd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25?	NYfqWXVZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
DCs	MoXOSpVv[3Srb36gRZN{[Xl?	M2jzTVIxyqEQvF2=	NIn3UFEyyqCq		NES2T3Rl\WO{ZXHz[ZMhUUxvMUKgdJJw\HWldHnvci=>	NWjVZm5mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N\|Q3OzZpPkK0OFM1PjN4PD;hQi=>
HUVEC	NWTPdXZ7TnWwY4Tpc44hSXO\|YYm=	MXiyNEDPxG4EoB?=	NVPrXIV3PSCq	MYLEUXNQ	NG\TZ4dz\WS3Y3XzJIN6fG:taX7lJIV5eHKnc4Ppc44hdGW4ZXzzJIlvKGFiY3;uZ4VvfHKjdHnvck1l\XCnbnTlcpQhdWGwbnXy	NHi1dHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4PVA3Oid-MkSxPFkxPjJ:L3G+
A 549	MonFSpVv[3Srb36gRZN{[Xl?	NYThVllFPTBizszN	NIjOWoQyKGh?		NH3sUFZl\WO{ZXHz[ZMhfGinIHzleoVtKG:oIFnMMVjDqA>?	NV;CdI8{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|k3QDhpPkK0NVc6Pjh6PD;hQi=>
H520	MlT0SpVv[3Srb36gRZN{[Xl?	MVi1M\|ExKM7:TR?=	M4HZRVEhcA>?	Mk\hSG1UVw>?	NGnHdI1l\WO{ZXHz[ZMhVVOKMjDwdo91\WmwIHHzJJdmdGxiYYOgcXJPSSCuZY\lcJMhcW5iZ3XmbZRqdmmkLXX4dI9{\WRiY3XscC=>	NUnITJhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN\|g6ODNpPkK0NVM5QTB\|PD;hQi=>
H1703	NGP3WZpHfW6ldHnvckBCe3OjeR?=	NXLXcmlJPS9zMDFOwG0>	NXH2XI5xOSCq	MoixSG1UVw>?	NVjnUpBm\GWlcnXhd4V{KE2VSEKgdJJwfGWrbjDhd{B4\WyuIHHzJI1TVkFibHX2[Yx{KGmwIHfl[ol1cW6rYj3lfJBwe2WmIHPlcIw>	MmD5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
H520	NF3ENHBHfW6ldHnvckBCe3OjeR?=	MXuxNOKhyrWP	MYqxNkBp	MY\EUXNQ	MUXk[YNz\WG\|ZYOgUXNJOiCvUl7BJIFv\CCycn;0[YlvKHO2YXLpcIl1gSCrbjDn[YZqfGmwaXKteJJm[XSnZDDOV2NNSyClZXzsdy=>	MoHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
H1703	MUTGeY5kfGmxbjDBd5NigQ>?	MYGxNOKhyrWP	NW\iZ4N6OTJiaB?=	NEPCVlNFVVOR	NEmwNIFl\WO{ZXHz[ZMhVVOKMjDtVm5CKGGwZDDwdo91\WmwIIP0ZYJqdGm2eTDpckBo\W[rdHnubYIufHKnYYTl[EBPW0OOQzDj[Yxtew>?	NWfVc49DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN\|g6ODNpPkK0NVM5QTB\|PD;hQi=>
MCF-7	M3S5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MV2xNOKh\|ryP	MnjyNlTDqGh?		NFK4TItqdmirYnn0d{B1cGViQ2KxNFgucW6mdXPl[EBk\WyuIHTlZZRp	M2jXelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUK4PFU{Lz5{NEGyPFg2OzxxYU6=
HPAEpiCs	MUDGeY5kfGmxbjDBd5NigQ>?	NYi5eFZNOC5zL{GvNVAh\|ryP	NEfCN4IyKGh?		NEmwSJVqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDjVGxCOsLicILveIVqdiCjbnSgcXJPSSCneIDy[ZN{cW:w	NX7nbXp2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOlkyPThpPkK0NFY6OTV6PD;hQi=>
podocytes	MXfGeY5kfGmxbjDBd5NigQ>?	M1nCcVExyqEQvF2=	MXmxJIg>		NF;0U4JqdmirYnn0d{BVT0cQskGtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB\|OF3BVGsh[W6mIFXyb\|EwOsLi	M2q0WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEO2NlEzLz5{NECzOlIyOjxxYU6=
MCF-7	M2fLVmZ2dmO2aX;uJGF{e2G7	Ml7VNVDDqM7:TR?=	MYexJIg>	MoKzSG1UVw>?	MmTpdoVlfWOnczD0bIUhX0FvaX7keYNm\CCyaH;zdIhwenmuYYTl[EBxOzhiTVHQTy=>	NGHzUGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxPVA6OCd-MkSwNVkxQTB:L3G+
MCF-7	MYTGeY5kfGmxbjDBd5NigQ>?	NYfjfpgzOTEEoN88US=>	NHqzSmQzPCCq	NF;VVXJFVVOR	NU\wboZTcW6lcnXhd4V{KHSqZTDXRU1qdmS3Y3XkJIFxd3C2b4Ppdy=>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzOUC5NEc,OjRyMUmwPVA9N2F-
HAPI	MWDGeY5kfGmxbjDBd5NigQ>?	M{HH[FExNzJyL{SwxsDPxE1?	Mm\CNUBp		M1fBd4lvcGmkaYTzJHRETERvaX7keYNm\CCyM{ivTm5MKE2DUFugdIhwe3Cqb4L5cIF1cW:w	NGfNcmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PVEzOCd-MkO5OlkyOjB:L3G+
HAPI	MWjGeY5kfGmxbjDBd5NigQ>?	NULIe\|ZEOjEEoN88UeKh	M3[xNFEhcA>?	NXHNcoRwTE2VTx?=	NFfjNGtifHSnboXheIV{KFSFRFStbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKGmQT2OgZY5lKHC{b3T1Z5Rqd25?	NHLJO\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PVEzOCd-MkO5OlkyOjB:L3G+
HepG2	MkHsSpVv[3Srb36gRZN{[Xl?	M{LGbVM2OCCwTR?=	MXmyOEBp		MmjabY5pcWKrdIOgeIhmKGSnZ4XlcIlvNWmwZIXj[YQh[WO2aY\heIlwdiCxZjDwN\|hOSVCN	NEeyV\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzN\|E6QCd-MkO5N\|MyQTh:L3G+
AGS	MUTGeY5kfGmxbjDBd5NigQ>?	MmXONVDDqM7:TR?=	M1XkZlMxKG2rbh?=		NGLDNWNqdmirYnn0d{Bk[XOyYYPlMVMh[WO2aY\heIlwdiCjbnSgbY5pcWKrdHnvckBw\iCHUlu=	M4TrUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEWwPVk1Lz5{M{i1NFk6PDxxYU6=
HepG2	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXvpPYRZOC13MDFOwG0>	NV;0cnp2PDhiaB?=		MojJbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK=	MmHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MEe1NFgoRjJ\|OEC3OVA5RC:jPh?=
BEL7404	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEDCclkxNTVyIN88US=>	MXm0PEBp		NUD1U4RRcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI>	M3vwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEC3OVA5Lz5{M{iwO\|UxQDxxYU6=
HL7702	M2PTOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mo[0NE02OCEQvF2=	NVPIXYRVPDhiaB?=		NYXaTmgxcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI>	NYmxTHRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NFc2ODhpPkKzPFA4PTB6PD;hQi=>
HepG2	M3TjfWZ2dmO2aX;uJGF{e2G7	MWiwMVUxKM7:TR?=	MnHBNlQhcA>?		M2\jbolvcGmkaYTzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZicEO4JIRwf26\|dILlZY0heHKxdHXpcpMhVUGSS1HQT\|ItKEGWRkKsJG1UUzFiYX7kJGhUWDJ5IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	NGXrRnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO\|UxQCd-MkO4NFc2ODh:L3G+
BEAS-2B	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NHrqfXMyOCEQvF2=	NH;ReWs{OCCvaX6=		NUP3Z2xGemW4ZYLz[ZMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgTGNK	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN\|Q9N2F-
BEAS-2B	Mkn5R5l1d3SxeHn0fUBCe3OjeR?=	NUf6TJdMOTBizszN	NX\JVGYxOzBibXnu		MXXpcohq[mm2ZXSgeIhmKGmwY4LlZZNmKGmwIFzETEBidmRiSVytPEBmgHC{ZYPzbY9v	MknBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OESwN\|QoRjJ\|N{i0NFM1RC:jPh?=
BEAS-2B	NEnZRVFHfW6ldHnvckBCe3OjeR?=	M4jzNVExKM7:TR?=	NXHaU\|lxOzBibXnu		M1XySIRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIHPhd5Bie2VvMzygRoFlKGGwZDDmZZM>	MlHtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OESwN\|QoRjJ\|N{i0NFM1RC:jPh?=
H9c2	NH;5fnBHfW6ldHnvckBCe3OjeR?=	NYj0RmxbOC5yMT:wMlEwOSEQvF2=	M4PTdFEhcA>?		NILmZYlifHSnboXheIV{KFSQRj5OtU1qdmS3Y3XkJG1OWC17IHX4dJJme3Orb36sJI1TVkFibHX2[Yx{NCCjbnSgdJJwdW:2ZYKgZYN1cX[rdIm=	NG\SUog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e3OFI2Oid-MkO3O\|QzPTJ:L3G+
H9c2	NHr2c\|RHfW6ldHnvckBCe3OjeR?=	MmDRNUDPxE1?	MYWxJIg>		NYLZT3ZoemWmdXPld{BVVkZvzsGg[Ilz\WO2bImgd5RqdXWuYYTl[EBxOzhiTVHQT{BxcG:\|cHjvdplt[XSrb36=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd5NEK1Nkc,OjN5N{SyOVI9N2F-
U937	MXvGeY5kfGmxbjDBd5NigQ>?	MY[xNOKh\|ryPwrC=	M{nPO\|EhcA>?		NV;vRXdX[WK{b3fheIV{KHSqZTDjZYZn\WmwZTDl[oZm[3Rib36gUWtRNTFiYX7kJHBROkGlzsGgcXJPSSC2cnHud4NzcXC2aX;uZYwhdGW4ZXzz	NUTyXVN6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc{QDdpPkKzO\|A4Ozh5PD;hQi=>
U937	NUjhWnZrTnWwY4Tpc44hSXO\|YYm=	NXvZco8zOTEEoN88UeKh	MVSxJIg>		MmXSZYJzd2ejdHXzJINi\m[naX7lMYlv\HWlZXSgUWtRNTFiZH;3ck1z\We3bHH0bY9vKGGwZDDQVFJC[87zIIXwMZJm\3WuYYTpc44>	NYTWS4V[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc{QDdpPkKzO\|A4Ozh5PD;hQi=>
U937	MlTpSpVv[3Srb36gRZN{[Xl?	MYOxNOKh\|ryPwrC=	M4X2TVEhcA>?		MUnzeZBxemW\|c3XzJIMuUnWwIHHu[EBEWkWEIIDoc5NxcG:{eXzheIlwdiCrbjDjZYZn\WmwZT30doVifGWmIHPlcIx{yqB?	Mo\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEezPFcoRjJ\|N{C3N\|g4RC:jPh?=
A549	NGWzN\|RHfW6ldHnvckBCe3OjeR?=	M{\FUlAvOy9\|L{OwJO69VQ>?	MkjKNUBp		NV;j[IRye2mpbnnmbYNidnSueTDheJRmdnWjdHXzJGFVWM7\|Uz3t[YRq[XSnZDDDU3guOiCycn;0[YlvKGGwZDDtVm5CKGW6cILld5Nqd25iYX7kJJBzd22xdHXyJIFkfGm4aYT5xsA>	MnLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEC2O\|QoRjJ\|NkiwOlc1RC:jPh?=
A549	M1W3dGZ2dmO2aX;uJGF{e2G7	MYCxNOKh\|ryPwrC=	MXewMVMxKG2rbh?=		NITibndqdmirYnn0d{BCXFEQs2OgbY5lfWOnZDDwOFIweDR2IF3BVGsh[W6mIICzPEBOSVCNIIDoc5NxcG:{eXzheIlwdg>?	NXzBXos5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549	M3nwS2Z2dmO2aX;uJGF{e2G7	NGLCTGEyOMLizszNxsA>	NFz1U2MyKGh?		MUnpcohq[mm2czDBWHDPu1NiaX7keYNm\CCQRj5OvmIheDZ3IIP1ZpVvcXRicHjvd5Bpd3K7bHH0bY9vKGGwZDDOSk3PwkJicILvcY91\XJiYXP0bZZqfHl?	M1nZTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkiwOlc1Lz5{M{[4NFY4PDxxYU6=
A549	M1\2VWZ2dmO2aX;uJGF{e2G7	MnPUNVDDqM7:TdMg	M2TlbVEhcA>?		NXq1SXVjemWmdXPld{BCXFEQs2Otd5RqdXWuYYTl[EBkWEyDMjDwbI9{eGixconsZZRqd28EoB?=	M2jBWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkiwOlc1Lz5{M{[4NFY4PDxxYU6=
A549	MmDUSpVv[3Srb36gRZN{[Xl?	MWixNOKh\|ryPwrC=	M4LZPFEhcA>?		MljmdoVlfWOnczDBWHDPu1NvZX7oZY5k\WRiZX76fY1ifGmlIHHjeIl3cXS7IH;mJINRVEF{wrC=	M3jCOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkiwOlc1Lz5{M{[4NFY4PDxxYU6=
PC12	MXfGeY5kfGmxbjDBd5NigQ>?	NUTJ[3B3OTBxMkCvOFAh\|ryP	M{PDRVHDqGh?		M4nsZ4lvcGmkaYTzJGpPUyCjbnSgdFM5	NX;TRYJNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PFQ{PTdpPkKzOVg1OzV5PD;hQi=>
HK-2	NIfxPVBCeG:ydH;zbZMhSXO\|YYm=	NEjmW2szOMLizszN	M4XGZlI1KGh?		M4DNW4lvcGmkaYTzJGVTUyCjbnSgdFM5VUGSSx?=	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV2M{G1NUc,OjN3NEOxOVE9N2F-
H9c2	NXvvVpl5TnWwY4Tpc44hSXO\|YYm=	NWT0SYdjOcLizszN	MYqxJIg>		M2jIe5Jm\HWlZYOgWG5HNc7zLXnu[JVk\WRiTV3QMVkhdVKQQTDs[ZZmdHNiYX7kJJBzd22xdHXyJIFkfGm4aYT5	MoX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NUO2PVkoRjJ\|M{WzOlk6RC:jPh?=
H9c2	NHLiNnhHfW6ldHnvckBCe3OjeR?=	NYn5NHJFOcLizszN	NGjtN4EyKGh?		MYjy[YR2[2W\|IGTOSk3PuS2nbnjhcoNm\CCDUD2xJJBzd22xdHXyJIFkfGm4aYT5	M4DyNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{WzOlk6Lz5{M{O1N\|Y6QTxxYU6=
H1650	MnPjSpVv[3Srb36gRZN{[Xl?	MVyxNOKh\|ryPwrC=	NX7XUGhTOSCq	MYfEUXNQ	MnzD[IVkemWjc3XzJIJwfGhicILveIVqdiCjbnSgcXJPSSCuZY\lcJMhd2ZiRWLDR\|EhcW5icHHjcIl1[XinbD3lfJBwe2WmwrDj[Yxtew>?	NVXpW3U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNlg3QTZpPkKzNlI5Pjl4PD;hQi=>
H1703	MmP1SpVv[3Srb36gRZN{[Xl?	NHfQc\|YyOMLizszNxsA>	NVHEZm1vOSCq	MXTEUXNQ	Mo[3[IVkemWjc3XzJIJwfGhicILveIVqdiCjbnSgcXJPSSCuZY\lcJMhd2ZiRWLDR\|EhcW5icHHjcIl1[XinbD3lfJBwe2WmwrDj[Yxtew>?	NEPDR409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KyPFY6Pid-MkOyNlg3QTZ:L3G+
H1650	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWKxNOKh\|ryPwrC=	NFPyOXIyKGh?	MnzaSG1UVw>?	MmnZ[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OE[5Okc,OjN{Mki2PVY9N2F-
H1703	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXmxNOKh\|ryPwrC=	M4ThdFEhcA>?	NHTYW4xFVVOR	MWjlcohidmOnczDwZYNtcXSjeHXsMYlv\HWlZXSgZ5l1d3SxeHnjbZR6	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ{OE[5Okc,OjN{Mki2PVY9N2F-
medulloblastoma cells	MnqwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				M1TOdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUA{NjByNjFOwG0v	MoqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N\|EoRjF5NEG3OlMyRC:jPh?=
neural precursor cells	NX\QbVRxSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				NXOyVWxOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W\|ZTDu[ZVz[WxicILlZ5Vze2:{IHPlcIx{KGK7IF3UWEBie3OjeTygSWM2OCB;IEiuNFY{KM7:TT6=	NFXKeYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxO\|Y{OSd-MUe0NVc3OzF:L3G+
RAW264.7	MnPwRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>?		M2nLWFExKG2rboO=		NYXsO5pXSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iaX7jeYJifGWmIH\vdkAyOCCvaX7zJJBzcW:{IITvJGxRWy2laHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhOThiaILzJIJ6KEe{aXXzd{Bu\XSqb3SsJGlEPTBiPTCxOkDPxE1w	M3riTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEOxO\|k5Lz5{MkizNVc6QDxxYU6=
BL21(DE3)	MWXGeY5kfGmxbjDhd5NigQ>?		MXW2NEBucW6\|		NVXv[5NEUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDOMZRmem2rbnHsJGhqezZvdHHn[4VlKEKURESg[ZhxemW\|c3XkJIlvKEW\|Y3jldolkcGmjIHPvcIkhSkx{MTjESVMqKGOnbHzzJJV{cW6pIHLpc5RqdnmuYYTl[EBpcXO2b37lJGg1KHCncITp[IUh[XNic4Xid5Rz[XSnIHHmeIVzKDZyIH3pcpMh[nliQXzwbIFU[3KnZX6gZZN{[XluIFnDOVAhRSB\|LkSg{txONg>?	NVW1S4p6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPVU4OjNpPkK4NVk2PzJ\|PD;hQi=>
RAW264.7	NV;3OYI{TnWwY4Tpc44h[XO\|YYm=				MkP0VJJwfGWldHnvckBi\2GrboP0JGJi[2mubIXzJIFvfGi{YXPpd{Bxem:2ZXP0bZZmKGGwdHnn[Y4h[W6mIHzleIhidCC2b4jpck1lcXCqdHjldoliKHSxeHnuJINpcW2ncnnjJJBzd3SnaX6gcYVlcWG2ZXSgZ5l1d3SxeHnjbZR6KGmwIH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W\|c3XkJIF{KGOnbHygeoli[mmuaYT5	NVfQe3hoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0PFU2ODRpPkG3OFg2PTB2PD;hQi=>
A673	MX;xTHRUKGG\|c3H5				NUXPRXJ3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFG2O\|Mh[2WubIO=	M4[0S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DAOY	MWnxTHRUKGG\|c3H5				NETXTohyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?=	Mkj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	MnfkdWhVWyCjc4PhfS=>				M2LyWZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCURDDj[Yxtew>?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
NB1643	NYXkPGdMeUiWUzDhd5NigQ>?				MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy\|	MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	M1nhNJFJXFNiYYPzZZk>				M3LncZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{	M37v[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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BT-37	NF;rRphyUFSVIHHzd4F6				NGGwbo1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1\|NzDj[Yxtew>?	NWLlN\|hMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
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OHS-50	Mmj0dWhVWyCjc4PhfS=>				MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJM>	NV6xTFFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
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SK-N-MC	M1zme5FJXFNiYYPzZZk>				NXvIWYFzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
NB1643	M{DvU5FJXFNiYYPzZZk>				MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORlE3PDNiY3XscJM>	MoT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	NWD6WFBmeUiWUzDhd5NigQ>?				MUjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{	NFnSWm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SJ-GBM2	MlXNdWhVWyCjc4PhfS=>				MkLtdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2ouT0KPMjDj[Yxtew>?	NUPjeVY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32	Mn3LdWhVWyCjc4PhfS=>				M4K0dpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM>	NGLhS449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
Rh18	MXTxTHRUKGG\|c3H5				NIrmUXNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDF6IHPlcIx{	NFfPWpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2 ; pJNK1 / pJNK2 / JNK1 / JNK2	26844273 18222647
Immunofluorescence	p-p38 / MMP9	24479681
Growth inhibition assay	Cell viability	26844273

In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. ^[5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. ^[8]

Protocol (from reference)

Kinase Assay: ^[1]	In vitro kinase assays: The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-³³P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-³³P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.
Cell Research: ^[2]	Cell lines: Jurkat, and HeLa Concentrations: Dissolved in DMSO, final concentrations ~50 μM Incubation Time: 24 hours Method: Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining. (Only for Reference)
Animal Research: ^[5]	Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG Dosages: 12.5 μg Administration: Administered via i.d. (Only for Reference)

References

+ Expand

Solubility (25°C)

In vitro	DMSO	66 mg/mL (199.19 mM)
	Ethanol	12 mg/mL (36.21 mM)
	Water	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 5%DMSO+40%PEG300+5%Tween80+50%H2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	8 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	331.34
Formula	C₂₀H₁₄N₃OF
CAS No.	152121-30-7
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

Download SB202190 (FHPI) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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