SB202190 (FHPI)

For research use only.

Catalog No.S1077

99 publications

SB202190 (FHPI) Chemical Structure

CAS No. 152121-30-7

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

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Selleck's SB202190 (FHPI) has been cited by 99 publications

Purity & Quality Control

Choose Selective p38 MAPK Inhibitors

Biological Activity

Description SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.
Targets
Ferroptosis [11]
()
p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. [2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. [3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). [4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. [6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 NGrrc4dHfW6ldHnvckBCe3OjeR?= MnHzNlUh|ryP NVP4U5A1OzBibXnu M1fRd2ROW00EoB?= NVH0XFRR[XS2ZX71ZZRmeyC2aHWgcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO|aX;uJI9nKGiERD2yJIlvKHKnc4DvcpNqfmVidH:gSGE> M{i3NVI3OjJ|MkWx
MDA-MB-231 NHnuVnFHfW6ldHnvckBCe3OjeR?= M4fjOFIh|ryP NUjSdppNOjRiaB?= NUfN[lVxdGW|c3Xu[ZMhS0OOMjDpcoR2[3Srb36gZpkhXE6IzsG= MX[yOlExODh2OB?=
rBMSCs M{DmXWZ2dmO2aX;uJGF{e2G7 MmrZNVAh|ryP NXXMS5BCOi53IHi= MYrk[ZBz\XO|ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\iCHUlugZY5lKHB|OB?= NEnWUlUzPjB3M{K2Oi=>
MG63 NX7y[oNLTnWwY4Tpc44hSXO|YYm= M3TwTVExNzJyL{OwJO69VQ>? NInlV4szPCCq M{fWVpNq\26rZnnjZY51dHliZHXjdoVie2W|IITo[UBt\X[nbDDv[kBxcG:|cHjvdplt[XSnZDDwN|ghcW6mdXPl[EBjgSCFZFPsNkBqdiCjIHPvcoNmdnS{YYTpc44hNWSncHXu[IVvfCCvYX7u[ZI> NFn5PIozPTl7OEOxNi=>
MG63 M3XTSGFxd3C2b4Ppd{BCe3OjeR?= Ml7pNVAwOjBxM{Cg{txO MkjnNlQhcA>? M3TlN5Nq\26rZnnjZY51dHliZHXjdoVie2WmIITo[UBieG:ydH;zbZMhemG2ZTDv[kBOTzZ|IHnu[JVk\WRiYomgR4REdDJ? NGnVTIwzPTl7OEOxNi=>
HTSMCs M3qw[WZ2dmO2aX;uJGF{e2G7 MkfqNE4yNzFxMUCg{txO M1q3TVEhcA>? Mn[ybY5pcWKrdHXkJGNQWk1vMj3pcoR2[2WmIFjPMVEheHKxdHXpckBt\X[nbIOgZY5lKG2UTlGg[ZhxemW|c3nvci=> MmKwNlU6OjF2NkS=
MIA PaCa-2 MWfGeY5kfGmxbjDBd5NigQ>? M4\yb|IxyqEQvF2= MV6yOEBp NXTxVIt5emWmdXPl[EBt[WO2YYTlJIFk[3WvdXzheIlwdiCrbjDjc41jcW6jdHnvckB4cXSqIHLveIghOi2GRzDhcoQhTC2jbHzvd4XDqA>? MorDNlU5QDh2OEm=
MIA PaCa-2 NYXtfXk3TnWwY4Tpc44hSXO|YYm= M2XHdVIxyqEQvF2= NYXacoVvOjRiaB?= NVu4XZVKemW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> MVmyOVg5QDR6OR?=
BxPC-3 MWrGeY5kfGmxbjDBd5NigQ>? M3Lld|IxyqEQvF2= NWS2XlFyOjRiaB?= MVvy[ZN2dHS|IHnuJIEhdW:mZYP0JIlvcGmkaYTpc44hd2ZiSFnGMVHPuSCycn;0[YlvKGGlY4XteYxifGmxbh?= MYOyOVg5QDR6OR?=
AsPC-1 MojSSpVv[3Srb36gRZN{[Xl? NXG0b2hrOjEEoN88US=> M13EbFI1KGh? NGLKcXJz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=> MmDUNlU5QDh2OEm=
MIA PaCa-2 NFX5WG5HfW6ldHnvckBCe3OjeR?= NUnWZYJDOjEEoN88US=> NIHPW2ozPCCq MWnlcohidmOnczDjcIVifmGpZTDv[kBRSVKSIIfo[Y4h[2:vYnnu[YQhf2m2aDDncJVkd3OnIHHuZYxw\3N? NV;yVm1KOjV6OEi0PFk>
MIA PaCa-2 NV3yUHdpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHUTm5yOjEEoN88US=> Ml\uNkBp NVnkdVRme2Wwc3n0bZpmeyClZXzsJIxqdmW|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On NFm2S|czPTh6OES4PS=>
BxPC-3 NXvEbIJjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVyyNOKh|ryP MV2yJIg> MVvz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> MXOyOVg5QDR6OR?=
AsPC-1 NFvvRVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUCyNOKh|ryP NGW3dXkzKGh? Mlfid4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm MmfUNlU5QDh2OEm=
HEY MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XvN|IxyqEQvF2= NIO2TYYzKGh? MVPz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> M3\IPFI2QDh6NEi5
OVCAR-3 NFrGUYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV[yNOKh|ryP NXTmfYF1OiCq NUe5[HlXe2Wwc3n0bZpmeyClZXzsJIxqdmW|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On M4fOdlI2QDh6NEi5
SK-OV-3 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWSyNOKh|ryP MUGyJIg> M1rtSpNmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? M{TVVFI2QDh6NEi5
MH7A  NIr4VXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\pPHgzPCCq M4CyZpJmcW6ob4Lj[ZMhfGinIHnubIljcXSxcomg[YZn\WO2czDv[kBZSU5? MkHSNlU5PjJ7Nk[=
MH7A  NWLOcFdqSXCxcITvd4l{KEG|c3H5 Mm\lNlUh|ryP M{\4PFI1KGh? NXvoS5JDemW4ZYLz[ZMh[2WubDDjfYNt\SCjcoLld5QhcW6mdXPl[EBjgSC[QV6gZY5lKGOjdYPl[EBieG:ydH;zbZMhd2ZiY3XscJMhfmmjIHHjeIl3[XSrb36gc4YhUk6N MYmyOVg3Ojl4Nh?=
SCC25 M13IOWZ2dmO2aX;uJGF{e2G7 MVuyNEDPxE1? MkTaNlQhcA>? MUXpcoNz\WG|ZYOgZZV1d3CqYXf5JIxmfmWu MnX6NlU5OzR2MEC=
HaCaT  MkfySpVv[3Srb36gRZN{[Xl? MWe1JOK2VQ>? NWfk[o1VOjRiaB?= MVnpcohq[mm2czDJSm4u|rNvaX7keYNm\CCFQ1yyNkBxem:mdXP0bY9vKGyndnXsdy=> MUOyOVg{PDN3Mx?=
HaCaT  MlWzSpVv[3Srb36gRZN{[Xl? MVW1JOK2VQ>? MnzaNlQhcA>? Mo\LbY5pcWKrdIOgTWZPNc7zLXnu[JVk\WRiQ1PMNlIheHKxZIXjeIlwdiCuZY\lcJM> NX7pZ|k4OjV6M{SzOVM>
HPAEpiCs  NHHXSo5HfW6ldHnvckBCe3OjeR?= Ml71NU8{NzFyIN88US=> MoTrNUBp NV7lOY1wemWmdXPld{BUOVBvaX7keYNm\CCLQ1HNMVEheHKxdHXpckBidmRibWLORUBmgHC{ZYPzbY9vKGGwZDDwdo9ud3SncjDhZ5Rqfmm2eR?= NGXofpEzPTd|NEmwNC=>
HPAEpiCs Mm\HSpVv[3Srb36gRZN{[Xl? NU\oVJg3OS9|L{GwJO69VQ>? M4LXdVEhcA>? NFj3NpZqdmirYnn0d{BUOVBvc4TpcZVt[XSnZDDBb5QheGixc4Doc5J6dGG2aX;uJEDDqA>? MX2yOVc{PDlyMB?=
HPAEpiCs MkPBSpVv[3Srb36gRZN{[Xl? NF\S[lcyNzNxMUCg{txO M3LqT|EhcA>? NELWWFlqdmirYnn0d{BUOVBidHnt[U1l\XCnbnTlcpRtgSC|dHnteYxifGWmIHOtTpVvKHCqb4PwbI9zgWyjdHnvci=> NIXMXGczPTd|NEmwNC=>
K562 NH\MRmlHfW6ldHnvckBCe3OjeR?= MWmxNOKh|ryP MkHBNeKhcA>? M2DQNGROW09? M1zkeIlvcGmkaYTzJJF2cW6jY4LpcoUucW6mdXPl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36= Mn7ONlU3QDRyNEO=
PANC-1 NFLDUIlHfW6ldHnvckBCe3OjeR?= NF;TdnkyOCEQvF2= NH;6XlcyKGh? MmPy[Y5p[W6lZYOgeIhmKGG3dH;wbIFocWNiZX\m[YN1yqB? NH6zO4gzPTZ|MkKyNi=>
BxPC-3 MYfGeY5kfGmxbjDBd5NigQ>? MnH0NVAh|ryP M4nSNFEhcA>? MYHlcohidmOnczD0bIUh[XW2b4DoZYdq[yCnZn\lZ5TDqA>? MVeyOVY{OjJ{Mh?=
K562  NYjQXG83TnWwY4Tpc44hSXO|YYm= M2\IdlAvOjVvMTFOwG0> M4XacVI1KGh? MYPzeZBxemW|c3XzJJJme3[ncnH0do9tNWmwZIXj[YQhUDKDWDDwbI9{eGixconsZZRqd25? MUeyOVYyQTN7Mh?=
THP-1 MX\GeY5kfGmxbjDBd5NigQ>? MVG1JOK2VQ>? MnrWNkBp NEPlNWx{cWewaX\pZ4FvfGy7IHH0eIVvfWG2ZYOgd4VkemW2aX;uJI9nKEmOLUJOtUBqdmS3Y3XkJIJ6KDJ5T1jDbI9tKHCudYOgSnNNNTIEoB?= Mnz4NlU2QTh4NkG=
WB NGHqRnVHfW6ldHnvckBCe3OjeR?= MX2yNQKBkc7:TdMg M3WxOVMx6oDLbXnu M3nM[YRm[3KnYYPld{B1cGViTGDTMUBweiCOVFGtbY5lfWOnZDDJUE03KGGwZDDUUmYu|rIEoIDyc4R2[3Srb36= NXm5bGNoOjV3M{C2PFI>
RAW 264.7 NHTJO2xHfW6ldHnvckBCe3OjeR?= MWixNOKh|ryP NWnHb2p4OzEkgJntbY4> MnrJbY5lfWOnczDjbIFz[WO2ZYLpd5Rq[yC4YXP1c4xifGmxbjDv[kBQS3QEoB?= MUOyOVQ3OTN7OR?=
RAW 264.7 NVz1WJNiTnWwY4Tpc44hSXO|YYm= MXyxNOKh|ryP MkCzN|DjiImvaX6= Mo[2ZZR1\W63YYTld{B1cGViZX\m[YN1eyCxZjDPVGchd25ib4P0[Y9kdGG|dDDy[ZRz[WO2aX;u MkO3NlU1PjF|OUm=
HaCaT  NGnITpNHfW6ldHnvckBCe3OjeR?= NXr6R29vPDEEoN88US=> M4KyOVMuOjRiaB?= MXTEUXNQ NWq1XlhLemWmdXPld{B1cGViYXPjeY12dGG2aX;uJI9nKFqRLUG= NIXLTI0zPTR|NUS4OS=>
H9c2 M2PY[mZ2dmO2aX;uJGF{e2G7 M4fBOVUxyqEQvF5CpC=> NEnuTXQyOiCq NHfK[mNz\WS3Y3XzJGxFUCC{ZXzlZZNmKGGwZDDNUXAhdG:|cx?= NF7LNnczPTJ2NUixPC=>
HSCs Mom0RZBweHSxc3nzJGF{e2G7 NIfB[2gzPSEQvF2= MXSyOOKhcA>? NIDwUmV{cWewaX\pZ4FvfGy7IHH0eIVvfWG2ZYOgWGcucW6mdXPl[EBi[3SrdnH0[YQhUFOFczDhdI9xfG:|aYO= M1TEPFI1QTZzOUWw
THP-1 NXXqRVZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHXO|IhcA>? M37zOmROW09? MlfhTWM2OD12LkhOwG0> M4TCXVI1QDF3MEi3
MDDCs NEezdVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUC3NkBp NEHvN3pFVVOR M3HncmlEPTB{LkhOwG0> M4LtclI1QDF3MEi3
MDDCs M{nZfmZ2dmO2aX;uJGF{e2G7 NFH3TlcxNTF3IN88US=> M1[0S|Q5KGh? NVXOZ5E6TE2VTx?= M{GzTJN2eHC{ZYPz[ZMhUU[QLd8xJIFv\CCLUD2xNEBxem:mdXP0bY9vyqB? NUjCRnRwOjR6MUWwPFc>
MDDCs M2Wxb2Z2dmO2aX;uJGF{e2G7 NIW2OXIxNTF3IN88US=> NE\WOI01QCCq NUDqR2I4TE2VTx?= M1nWPIlvcGmkaYTzJG1KWC1zYTygUWlRNTGkIHHu[EBTSU6WRWOgdJJw\HWldHnvci=> M2L5VVI1QDF3MEi3
MDDCs MmTzSpVv[3Srb36gRZN{[Xl? NXvLblVvOTBizszN NIHhfWo{NjViaB?= M3zBeGROW09? M4HyXIJtd2OtczDFRm9XKEeSLDDieZQhdm:2IG\TWkBIKG2nZHnheIVlKGWwdIL5JIlvfG9iaIXtZY4hVUSGQ4O= NV;HVG46OjR6MUWwPFc>
macrophages NWn1VJVnTnWwY4Tpc44hSXO|YYm= NHnrVXUy6oDLzszN MUm0MlXjiImq M{jmXoNwdXCuZYTlcJkhcW6qaXLpeJMhVVRvSVnJMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCQRj5OvmI> MW[yOFgxQDZ|Mx?=
PDL  MYjGeY5kfGmxbjDBd5NigQ>? MXKyNOKh|ryPwrC= NV22S5FROzBibXnu NHvidYpFVVOR NFrGXIp{cWewaX\pZ4FvfGy7IHnubIljcXS|IITo[UB1\W6|aXzlJIZwemOnLX3l[IlifGWmIFLNVE0zKGW6cILld5Nqd25? Mle4NlQ2PjFyOEG=
AGS Mn7NSpVv[3Srb36gRZN{[Xl? M2XQbVUh|ryP NGm5dYM{OCCvaX6= Mlf6d5VxeHKnc4Pld:KhOS2rbnT1Z4VlKGOjc4Dhd4UuQCCjbnSgZ4F{eGG|ZT2zJIFkfGm4YYTpc44> M17qSVI1PTR5OEe4
H520 NIPsPW1HfW6ldHnvckBCe3OjeR?= M1vmcVExyqEEtV2= MWSxJIg> NI\yZnNFVVOR MVvk[YNz\WG|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy| MWKyOFU{ODR5NR?=
H1703 NXzM[2VHTnWwY4Tpc44hSXO|YYm= MUmxNOKhyrWP NHvVSmoyKGh? NHflXVdFVVOR M2PEOIRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO= NGm2cFgzPDV|MES3OS=>
H520 MYPGeY5kfGmxbjDBd5NigQ>? NHjFZ2EyOMLiwsXN NHPQflgyOiCq NXjqWXhFTE2VTx?= NYnofoZscW6qaXLpeJMheGWvZYTy[Zhm\C2nbHnjbZRm\CCPU1iyJJBzd3SnaX6gd5Ri[mmuaYT5 MoLmNlQ2OzB2N{W=
H1703 NFP4eotHfW6ldHnvckBCe3OjeR?= M3fPZVExyqEEtV2= MljqNVIhcA>? NWTZTmdGTE2VTx?= NXjFT4NkcW6qaXLpeJMheGWvZYTy[Zhm\C2nbHnjbZRm\CCPU1iyJJBzd3SnaX6gd5Ri[mmuaYT5 MXeyOFU{ODR5NR?=
H520 NX\sOXZqTnWwY4Tpc44hSXO|YYm= M4rKR|ExyqEEtV2= NXr3OmhxPiCq NUDGd2d6TE2VTx?= NHnnfoJ{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUhdGW4ZXzzJI9nKHWkaYH1bZRqdi2lb37qeYdifGWmIF3TTFIhcW5icHXt[ZRz\XinZD30doVifGWmwrDj[YxtKGyrbnW= M2nXblI1PTNyNEe1
H1703 MVfGeY5kfGmxbjDBd5NigQ>? MnvuNVDDqML3TR?= MWO2JIg> NFHB[I1FVVOR NYfldlY2e2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l MlrnNlQ2OzB2N{W=
MC3T3-E1 M3PSbmZ2dmO2aX;uJGF{e2G7 M4rPVVAvOy9|L{OwJO69VQ>? NVHmepBDOSCq M2XxV4F1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nctMg MWiyOFUxOjZ7Nh?=
MC3T3-E1 NFqyWpZHfW6ldHnvckBCe3OjeR?= MnLNN|Ah|ryP Mkf2NUBp MUPheJRmdnWjdHXzJHRPTi4QsT3zeIlufWyjdHXkJJA{QCCPQWDLJJBpd3OyaH;yfYxifGmxbh?= M{\5eVI1PTB{Nkm2
HUVECs  MoTvSpVv[3Srb36gRZN{[Xl? MUexNOKhyrWP M1XKTVEhcA>? MWTpcohq[mm2czDUUmYu|rFvaX7keYNm\CCFWFPMNUBxem:mdXP0bY9v MWCyOFQ5Pzl4NB?=
AGS  NVnsbVNkTnWwY4Tpc44hSXO|YYm= MkLtNVDDqML3TR?= NHrNW2c{OMLibXnu MYnpcohq[mm2czDJUE0y|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>? MmixNlQ1Pzl4OEG=
MKN-45 MnHISpVv[3Srb36gRZN{[Xl? MYSxNOKhyrWP NEH6eFU{OMLibXnu M{XZWIlvcGmkaYTzJGlNNTIQsj3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6 NVT2PHlVOjR2N{m2PFE>
AGS  NVzwWod5TnWwY4Tpc44hSXO|YYm= MnLFNVDDqML3TR?= NXX0ZWFFOzEEoH3pci=> NVjOcGNy[XS2ZX71ZZRmeyCLTD2x{tIucW6mdXPl[EBISSClZXzsJI1q\3KjdHnvckBidmRiaX72ZZNqd25? M1PMS|I1PDd7Nkix
MKN-45 MmfxSpVv[3Srb36gRZN{[Xl? NWrjOJZLOTEEoNM1US=> MVuzNOKhdWmw MX3heJRmdnWjdHXzJGlNNTIQsj3pcoR2[2WmIFfBJINmdGxibXnndoF1cW:wIHHu[EBqdn[jc3nvci=> NWi5VnlMOjR2N{m2PFE>
AGS  MXzGeY5kfGmxbjDBd5NigQ>? MVmxNOKhyrWP MorrN|DDqG2rbh?= MUTzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCLbD2x{tIucW6mdXPl[EBOVVB{IHHu[EBOVVB7IH3SUmEh\XiycnXzd4lwdg>? M{D0WlI1PDd7Nkix
MKN-45 NV\ZZWRsTnWwY4Tpc44hSXO|YYm= NYrtVZdWOTEEoNM1US=> M4jUWVMxyqCvaX6= MWPzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCLbD2x{tIucW6mdXPl[EBOVVB{IHHu[EBOVVB7IH3SUmEh\XiycnXzd4lwdg>? NYfoXnA1OjR2N{m2PFE>
DCs NF3FW5VHfW6ldHnvckBCe3OjeR?= Mn\QNlDDqM7:TR?= Ml;UNeKhcA>? MlPH[IVkemWjc3XzJGlNNTF{IIDyc4R2[3Srb36= NXmycI84OjR2M{S2N|Y>
HUVEC  MVfGeY5kfGmxbjDBd5NigQ>? M3nyV|IxKM7:bdMg MV21JIg> MXrEUXNQ M{\IfpJm\HWlZYOgZ5l1d2urbnWg[ZhxemW|c3nvckBt\X[nbIOgbY4h[SClb37j[Y51emG2aX;uMYRmeGWwZHXueEBu[W6wZYK= NWTvNYdHOjRzOEmwOlI>
A 549 NVixdJF3TnWwY4Tpc44hSXO|YYm= M{Dy[|UxKM7:TR?= MonaNUBp NUXmZYRO\GWlcnXhd4V{KHSqZTDs[ZZmdCCxZjDJUE05yqB? Ml6wNlQyPzl4OEi=
H520  NYf0fms5TnWwY4Tpc44hSXO|YYm= M3XYdlUwOTBizszN MmjyNUBp M1PiOWROW09? NYnPbnR4\GWlcnXhd4V{KE2VSEKgdJJwfGWrbjDhd{B4\WyuIHHzJI1TVkFibHX2[Yx{KGmwIHfl[ol1cW6rYj3lfJBwe2WmIHPlcIw> NFHudGszPDF|OEmwNy=>
H1703  NF[2eplHfW6ldHnvckBCe3OjeR?= MWq1M|ExKM7:TR?= M4L5SlEhcA>? NX\rXXRYTE2VTx?= NF[4N|dl\WO{ZXHz[ZMhVVOKMjDwdo91\WmwIHHzJJdmdGxiYYOgcXJPSSCuZY\lcJMhcW5iZ3XmbZRqdmmkLXX4dI9{\WRiY3XscC=> MUOyOFE{QDlyMx?=
H520  MonqSpVv[3Srb36gRZN{[Xl? M2XuclExyqEEtV2= MVGxNkBp M4TvPWROW09? NUDGS5ZK\GWlcnXhd4V{KE2VSEKgcXJPSSCjbnSgdJJwfGWrbjDzeIFjcWyrdImgbY4h\2WoaYTpcoljNXS{ZXH0[YQhVlOFTFOgZ4VtdHN? MnTDNlQyOzh7MEO=
H1703  NIHNNFNHfW6ldHnvckBCe3OjeR?= MVKxNOKhyrWP NWXlNFZWOTJiaB?= NHzROlhFVVOR NUjkU5lw\GWlcnXhd4V{KE2VSEKgcXJPSSCjbnSgdJJwfGWrbjDzeIFjcWyrdImgbY4h\2WoaYTpcoljNXS{ZXH0[YQhVlOFTFOgZ4VtdHN? NIHMdpAzPDF|OEmwNy=>
MCF-7  NXPWNGtIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUSxNOKh|ryP MVmyOOKhcA>? NF\ydZJqdmirYnn0d{B1cGViQ2KxNFgucW6mdXPl[EBk\WyuIHTlZZRp M2HOcVI1OTJ6OEWz
HPAEpiCs NX21OXVGTnWwY4Tpc44hSXO|YYm= MnHCNE4yNzFxMUCg{txO NGDh[5IyKGh? NI\zS5dqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDjVGxCOsLicILveIVqdiCjbnSgcXJPSSCneIDy[ZN{cW:w NF3sfZUzPDB4OUG1PC=>
podocytes NVfa[VBITnWwY4Tpc44hSXO|YYm= M3jGc|ExyqEQvF2= NU\VSpozOSCq M37IOIlvcGmkaYTzJHRITs7{MT3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6TVHQT{BidmRiRYLrNU8zyqB? NVK5U2FyOjRyM{[yNVI>
MCF-7 NHzLdWdHfW6ldHnvckBCe3OjeR?= M3PjNFExyqEQvF2= M2HpVlEhcA>? NFzoSWFFVVOR MnK2doVlfWOnczD0bIUhX0FvaX7keYNm\CCyaH;zdIhwenmuYYTl[EBxOzhiTVHQTy=> M1voUlI1ODF7MEmw
MCF-7 M2TpPWZ2dmO2aX;uJGF{e2G7 NWn2[5E1OTEEoN88US=> NHy2S3kzPCCq MonvSG1UVw>? M{ixbIlv[3KnYYPld{B1cGViV1GtbY5lfWOnZDDhdI9xfG:|aYO= MoLyNlQxOTlyOUC=
HAPI NF6yXlFHfW6ldHnvckBCe3OjeR?= M2Pxc|ExNzJyL{SwxsDPxE1? NEPQTGMyKGh? NVX0So9rcW6qaXLpeJMhXEOGRD3pcoR2[2WmIICzPE9LVktiTVHQT{BxcG:|cHjvdplt[XSrb36= Mmj4NlM6PjlzMkC=
HAPI NIG0N2dHfW6ldHnvckBCe3OjeR?= M4TIb|IxyqEQvF5CpC=> MXKxJIg> NUHTUY5VTE2VTx?= M1[1PIF1fGWwdXH0[ZMhXEOGRD3pcoR2[2WmIHHjeIl3[XSrb36gc4YhcU6RUzDhcoQheHKxZIXjeIlwdg>? M1H6R|I{QTZ7MUKw
HepG2 NUn1TZJVTnWwY4Tpc44hSXO|YYm= NFrR[o8{PTBibl2= MlvwNlQhcA>? MYDpcohq[mm2czD0bIUh\GWpdXXsbY4ucW6mdXPl[EBi[3SrdnH0bY9vKG:oIICzPG1CWEt? M1LqNVI{QTN|MUm4
AGS  M2jNPWZ2dmO2aX;uJGF{e2G7 MnvINVDDqM7:TR?= NETVSWo{OCCvaX6= NHrYTYZqdmirYnn0d{Bk[XOyYYPlMVMh[WO2aY\heIlwdiCjbnSgbY5pcWKrdHnvckBw\iCHUlu= NHjMZ3UzOzh3MEm5OC=>
HepG2 MmTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETFcWwxNTVyIN88US=> Mk\OOFghcA>? MU\pcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= MnTzNlM5ODd3MEi=
BEL7404 M3i4Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLEOplvOC13MDFOwG0> M{HwblQ5KGh? MWHpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= M2rEblI{QDB5NUC4
HL7702 MnfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnNNYwxNTVyIN88US=> Ml\1OFghcA>? MkO4bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NVPPd|JFOjN6MEe1NFg>
HepG2 NF3ofnVHfW6ldHnvckBCe3OjeR?= MYWwMVUxKM7:TR?= NITiNVQzPCCq MWXpcohq[mm2czD0bIUheGixc4Doc5J6dGG2aX;uJI9nKHB|ODDkc5dve3S{ZXHtJJBzd3SnaX7zJG1CWEuDUFuyMEBCXEZ{LDDNV2syKGGwZDDIV3AzPyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M3O2NlI{QDB5NUC4
BEAS-2B Mkf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\lUXk3OTBizszN NWHlW5FJOzBibXnu NHzscYFz\X[ncoPld{B1cGViZHXjdoVie2Vib3[gZ4VtdCC4aXHibYxqfHliaX7keYNm\CCkeTDIR2k> MmLyNlM4QDRyM{S=
BEAS-2B MYfDfZRwfG:6aYT5JGF{e2G7 M2\4UFExKM7:TR?= NX\JWHNOOzBibXnu MUXpcohq[mm2ZXSgeIhmKGmwY4LlZZNmKGmwIFzETEBidmRiSVytPEBmgHC{ZYPzbY9v NIXPW4gzOzd6NECzOC=>
BEAS-2B MXHGeY5kfGmxbjDBd5NigQ>? MkDuNVAh|ryP NWn1RpFTOzBibXnu NIHv[Vdl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDjZZNx[XOnLUOsJGJi\CCjbnSg[oF{ NIjSUXczOzd6NECzOC=>
H9c2  NH;GW4NHfW6ldHnvckBCe3OjeR?= M2O0S|AvODFxMD6xM|Eh|ryP MUGxJIg> M4X1PIF1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjygcXJPSSCuZY\lcJMtKGGwZDDwdo9ud3SncjDhZ5Rqfmm2eR?= NVjrdIpDOjN5N{SyOVI>
H9c2  MVrGeY5kfGmxbjDBd5NigQ>? MkS5NUDPxE1? NXvw[VRZOSCq MoHUdoVlfWOnczDUUmYu|rFiZHny[YN1dHlic4TpcZVt[XSnZDDwN|ghVUGSSzDwbI9{eGixconsZZRqd25? Ml3UNlM4PzR{NUK=
U937 M13GWWZ2dmO2aX;uJGF{e2G7 NXe5RWVrOTEEoN88UeKh MnyzNUBp MUnhZpJw\2G2ZYOgeIhmKGOjZn\lbY5mKGWoZnXjeEBwdiCPS2CtNUBidmRiUGCyRYPPuSCvUl7BJJRz[W6|Y4LpdJRqd26jbDDs[ZZmdHN? Mm\xNlM4ODd|OEe=
U937 MWXGeY5kfGmxbjDBd5NigQ>? NGLEbHEyOMLizszNxsA> M2\yOFEhcA>? MnLKZYJzd2ejdHXzJINi\m[naX7lMYlv\HWlZXSgUWtRNTFiZH;3ck1z\We3bHH0bY9vKGGwZDDQVFJC[87zIIXwMZJm\3WuYYTpc44> NVW1cG1POjN5MEezPFc>
U937 MnTwSpVv[3Srb36gRZN{[Xl? NYPoSoVCOTEEoN88UeKh MmTENUBp NWnSN2xWe3WycILld5NmeyClLVr1ckBidmRiQ2LFRkBxcG:|cHjvdplt[XSrb36gbY4h[2GoZnXpcoUufHKnYYTl[EBk\Wyuc9Mg NGXNR|AzOzdyN{O4Oy=>
A549 NX7UOo9KTnWwY4Tpc44hSXO|YYm= NXvlPFRuOC5|L{OvN|Ah|ryP NH7FTZEyKGh? NITRbnN{cWewaX\pZ4FvfGy7IHH0eIVvfWG2ZYOgRXRR|rOVLX3l[IlifGWmIFPPXE0zKHC{b4TlbY4h[W6mIH3SUmEh\XiycnXzd4lwdiCjbnSgdJJwdW:2ZYKgZYN1cX[rdIpCpC=> NHHHVVczOzZ6ME[3OC=>
A549 M2WxPWZ2dmO2aX;uJGF{e2G7 MlXFNVDDqM7:TdMg NVm4bYdwOC1|MDDtbY4> MnTDbY5pcWKrdIOgRXRR|rOVIHnu[JVk\WRicESyM5A1PCCPQWDLJIFv\CCyM{igUWFRUyCyaH;zdIhwenmuYYTpc44> NYHPSG52OjN4OEC2O|Q>
A549 NYfNO5pwTnWwY4Tpc44hSXO|YYm= NF3LT|cyOMLizszNxsA> NEXu[GEyKGh? NVvmPFdrcW6qaXLpeJMhSVSSzsPTJIlv\HWlZXSgUmYu|rqEIIC2OUB{fWK3bnn0JJBpd3OyaH;yfYxifGmxbjDhcoQhVkZvzsrCJJBzd22xdHXyJIFkfGm4aYT5 NYTzbWxZOjN4OEC2O|Q>
A549 NInyXY9HfW6ldHnvckBCe3OjeR?= MXexNOKh|ryPwrC= MljkNUBp MljBdoVlfWOnczDBWHDPu1Nvc4TpcZVt[XSnZDDjVGxCOiCyaH;zdIhwenmuYYTpc47DqA>? MkXrNlM3QDB4N{S=
A549 NWTtR5NCTnWwY4Tpc44hSXO|YYm= MUmxNOKh|ryPwrC= M{SxfFEhcA>? NH\tNGxz\WS3Y3XzJGFVWM7|Uz3lcohidmOnZDDlcpp6dWG2aXOgZYN1cX[rdImgc4Yh[1COQUNCpC=> M4fH[VI{PjhyNke0
PC12 M4PRUWZ2dmO2aX;uJGF{e2G7 NGPGR4EyOC9{MD:0NEDPxE1? NHHFXHcyyqCq NY\MV5BkcW6qaXLpeJMhUk6NIHHu[EBxOzh? NXLUOFJtOjN3OESzOVc>
HK-2 MUXBdI9xfG:|aYOgRZN{[Xl? M1npTVIxyqEQvF2= M{fkSVI1KGh? MYjpcohq[mm2czDFVmsh[W6mIICzPG1CWEt? NWi2Z2x{OjN3NEOxOVE>
H9c2 NIXDdnNHfW6ldHnvckBCe3OjeR?= M{nZZ|HDqM7:TR?= M13F[|EhcA>? MoXHdoVlfWOnczDUUmYu|rFvaX7keYNm\CCPTWCtPUBuWk6DIHzleoVteyCjbnSgdJJwdW:2ZYKgZYN1cX[rdIm= NYTDN4kyOjN|NUO2PVk>
H9c2 MknBSpVv[3Srb36gRZN{[Xl? M{LrdlHDqM7:TR?= NHr0NmgyKGh? Mn3TdoVlfWOnczDUUmYu|rFvZX7oZY5k\WRiQWCtNUBxem:vb4TldkBi[3Srdnn0fS=> NWDqeWFyOjN|NUO2PVk>
H1650  MmH5SpVv[3Srb36gRZN{[Xl? MkHSNVDDqM7:TdMg M{fOT|EhcA>? NVr0N4tmTE2VTx?= Mo\i[IVkemWjc3XzJIJwfGhicILveIVqdiCjbnSgcXJPSSCuZY\lcJMhd2ZiRWLDR|EhcW5icHHjcIl1[XinbD3lfJBwe2WmwrDj[Yxtew>? M{S5R|I{OjJ6Nkm2
H1703 M3rGPGZ2dmO2aX;uJGF{e2G7 MmDiNVDDqM7:TdMg Mo\yNUBp M3rDXWROW09? NInRR29l\WO{ZXHz[ZMh[m:2aDDwdo91\WmwIHHu[EBuWk6DIHzleoVteyCxZjDFVmNEOSCrbjDwZYNtcXSjeHXsMYV5eG:|ZXVCpINmdGy| M{HRdFI{OjJ6Nkm2
H1650  NF3IdG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX[xNOKh|ryPwrC= MXOxJIg> NGe3TZpFVVOR MmDY[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>? M3[2elI{OjJ6Nkm2
H1703 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfjNVDDqM7:TdMg M{DJW|EhcA>? M3:yVmROW09? MljH[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>? NF\sSmUzOzJ{OE[5Oi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2; 

PubMed: 26844273     


CRC cells were treated with 10 μM SB202190 for 2 h and analyzed for mTOR signaling and on-target inhibition of p38 by immunoblot. 

pJNK1 / pJNK2 / JNK1 / JNK2 ; 

PubMed: 18222647     


A549 cells were treated with DMSO control or with 10 µM of SB202190 for 0.5, 1, 2, 3, 4, or 6 hours, and pJNK and unphosphorylated JNK were detected as described. 

26844273 18222647
Immunofluorescence
p-p38 / MMP9; 

PubMed: 24479681     


Immunocytochemical staining and confocal microscopy confirmed that IL-1β increased activation of p38 (red), and MMP2 and MMP9 (green) expression in MKN-45 GA cells; these effects were blocked by p38 siRNA or the p38 inhibitor SB202190.

24479681
Growth inhibition assay
Cell viability ; 

PubMed: 26844273     


Top panel: CRC cells were treated with various concentrations of SB202190 for 48 h. Lower panel: CRC cells were treated with SB202190 at 10 μM for different times. Cell growth was measured by SRB assay (means ± s.d. in triplet, n = 3 in triplet).

26844273
In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. [5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. [8]

Protocol

Kinase Assay:

[1]

- Collapse

In vitro kinase assays:

The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-33P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-33P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.
Cell Research:

[2]

- Collapse
  • Cell lines: Jurkat, and HeLa
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.


    (Only for Reference)
Animal Research:

[5]

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  • Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG
  • Dosages: 12.5 μg
  • Administration: Administered via i.d.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (199.19 mM)
Ethanol 12 mg/mL (36.21 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%H2O
For best results, use promptly after mixing.
8 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.34
Formula

C20H14N3OF

CAS No. 152121-30-7
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID