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SB202190 (FHPI)

Name: SB202190 (FHPI)
Brand: Selleck Chemicals
SKU: S1077
Rating: 5 (141 reviews)

Catalog No.S1077

For research use only.

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

CAS No. 152121-30-7

Selleck's SB202190 (FHPI) has been cited by 141 publications

Purity & Quality Control

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p38 MAPK Signaling Pathway Map

Biological Activity

Description

Targets

Ferroptosis ^[11]	p38α ^[1] (Cell-free assay)	p38β ^[1] (Cell-free assay)
	50 nM	100 nM

In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. ^[2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. ^[3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). ^[4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. ^[6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. ^[7]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

HCT-116	NFrlNJVHfW6ldHnvckBCe3OjeR?=	NF;JOGgzPSEQvF2=	MYizNEBucW5?	NVn1NHhnTE2VT9Mg	NF\DcmRifHSnboXheIV{KHSqZTDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36gc4YhcEKGLUKgbY4hemW\|cH;ud4l3\SC2bzDERS=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{M{K1NUc,OjZ{MkOyOVE9N2F-
MDA-MB-231	MXLGeY5kfGmxbjDBd5NigQ>?	M4nUeVIh\|ryP	NGHNZlYzPCCq		MoTCcIV{e2WwZYOgR2NNOiCrbnT1Z5Rqd25iYomgWG5H\|rF?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFyMEi0PEc,OjZzMEC4OFg9N2F-
rBMSCs	MX3GeY5kfGmxbjDBd5NigQ>?	MmPjNVAh\|ryP	MWKyMlUhcA>?		Mn3m[IVxemW\|c3XzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiRWLLJIFv\CCyM{i=	M3myOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEWzNlY3Lz5{NkC1N\|I3PjxxYU6=
MG63	M3fUcGZ2dmO2aX;uJGF{e2G7	NHTlVHIyOC9{MD:zNEDPxE1?	M{f5TFI1KGh?		M2m0RZNq\26rZnnjZY51dHliZHXjdoVie2W\|IITo[UBt\X[nbDDv[kBxcG:\|cHjvdplt[XSnZDDwN\|ghcW6mdXPl[EBjgSCFZFPsNkBqdiCjIHPvcoNmdnS{YYTpc44hNWSncHXu[IVvfCCvYX7u[ZI>	M3\nVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUm4N\|EzLz5{NUm5PFMyOjxxYU6=
MG63	NVvM[ZA1SXCxcITvd4l{KEG\|c3H5	Ml;oNVAwOjBxM{Cg{txO	Ml7ENlQhcA>?		NWnS[VBRe2mpbnnmbYNidnSueTDk[YNz\WG\|ZXSgeIhmKGGyb4D0c5NqeyC{YYTlJI9nKE2JNkOgbY5lfWOnZDDifUBE\EOuMh?=	NWjRSpY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PVg{OTJpPkK1PVk5OzF{PD;hQi=>
HTSMCs	M2TjZmZ2dmO2aX;uJGF{e2G7	NY\XXlRHOC5zL{GvNVAh\|ryP	M3;YZVEhcA>?		NGH6NmlqdmirYnn0[YQhS0:UTT2yMYlv\HWlZXSgTG8uOSCycn;0[YlvKGyndnXsd{BidmRibWLORUBmgHC{ZYPzbY9v	NG\GS\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUmyNVQ3PCd-MkW5NlE1PjR:L3G+
MIA PaCa-2	NYDjOGdNTnWwY4Tpc44hSXO\|YYm=	MYeyNOKh\|ryP	NHHmVHAzPCCq		MoHQdoVlfWOnZDDsZYN1[XSnIHHjZ5VufWyjdHnvckBqdiClb33ibY5ifGmxbjD3bZRpKGKxdHigNk1FTyCjbnSgSE1idGyxc3ZCpC=>	MmnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
MIA PaCa-2	M{Lhc2Z2dmO2aX;uJGF{e2G7	Mn:yNlDDqM7:TR?=	M13STFI1KGh?		NI\aUHFz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=>	Mme4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
BxPC-3	MnjsSpVv[3Srb36gRZN{[Xl?	M3zJ[VIxyqEQvF2=	M3e4VlI1KGh?		NYjqW4FUemW\|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44>	NEfTSog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
AsPC-1	NE\LXXZHfW6ldHnvckBCe3OjeR?=	NXjY[WFGOjEEoN88US=>	M3i1[VI1KGh?		MoTLdoV{fWy2czDpckBiKG2xZHXzeEBqdmirYnn0bY9vKG:oIFjJSk0y\|rFicILveIVqdiCjY3P1cZVt[XSrb36=	NGjqR2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2	MnrqSpVv[3Srb36gRZN{[Xl?	NYCyPY1LOjEEoN88US=>	NHLDS3MzPCCq		NFvJSVRmdmijbnPld{BkdGWjdnHn[UBw\iCSQWLQJJdp\W5iY3;tZolv\WRid3n0bEBodHWlb4PlJIFv[WyxZ4O=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
MIA PaCa-2	NWCx[VRLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MYOyNOKh\|ryP	MV[yJIg>		NH\vPIV{\W6\|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V?	NUfuTHl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
BxPC-3	NXK1[JF2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkPzNlDDqM7:TR?=	MmfkNkBp		NFewb\|N{\W6\|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
AsPC-1	NYLZeYJPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHXsRlYzOMLizszN	M{TLNVIhcA>?		NWexNY9Re2Wwc3n0bZpmeyClZXzsJIxqdmW\|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On	Mo\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
HEY	Ml;GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWOyNOKh\|ryP	NH3PN5QzKGh?		M2CxdZNmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>?	NUX2Z4h6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
OVCAR-3	M1rsVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MUmyNOKh\|ryP	MlyyNkBp		Moqzd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm	Mk\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
SK-OV-3	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{fuRlIxyqEQvF2=	M2m1cVIhcA>?		NUD5WnJ4e2Wwc3n0bZpmeyClZXzsJIxqdmW\|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On	M4rMb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
MH7A	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MnLPNlQhcA>?		MYLy[Ylv\m:{Y3XzJJRp\SCrbnjpZol1d3K7IHXm[oVkfHNib3[gXGFP	NIry[m49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2Nlk3Pid-MkW4OlI6PjZ:L3G+
MH7A	MmfrRZBweHSxc3nzJGF{e2G7	MoDxNlUh\|ryP	MXWyOEBp		Mo\TdoV3\XK\|ZYOgZ4VtdCCleXPs[UBienKnc4SgbY5lfWOnZDDifUBZSU5iYX7kJINifXOnZDDhdI9xfG:\|aYOgc4Yh[2WubIOgeoliKGGldHn2ZZRqd25ib3[gTm5M	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh4Mkm2Okc,OjV6NkK5OlY9N2F-
SCC25	NUfCfpZRTnWwY4Tpc44hSXO\|YYm=	Mlq1NlAh\|ryP	MXWyOEBp		Mn7RbY5kemWjc3XzJIF2fG:yaHHnfUBt\X[nbB?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh\|NESwNEc,OjV6M{S0NFA9N2F-
HaCaT	M2nhVWZ2dmO2aX;uJGF{e2G7	MnPxOUDDvU1?	M1T5RlI1KGh?		NYXOcG1LcW6qaXLpeJMhUU[QLd8zMYlv\HWlZXSgR2NNOjJicILv[JVkfGmxbjDs[ZZmdHN?	NV3RXlh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N\|Q{PTNpPkK1PFM1OzV\|PD;hQi=>
HaCaT	NIe0RZpHfW6ldHnvckBCe3OjeR?=	M4rxN\|UhyrWP	MWCyOEBp		MnflbY5pcWKrdIOgTWZPNc7zLXnu[JVk\WRiQ1PMNlIheHKxZIXjeIlwdiCuZY\lcJM>	MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6M{SzOVMoRjJ3OEO0N\|U{RC:jPh?=
HPAEpiCs	M4\DO2Z2dmO2aX;uJGF{e2G7	MnXjNU8{NzFyIN88US=>	Mm\rNUBp		NXO0N5VGemWmdXPld{BUOVBvaX7keYNm\CCLQ1HNMVEheHKxdHXpckBidmRibWLORUBmgHC{ZYPzbY9vKGGwZDDwdo9ud3SncjDhZ5Rqfmm2eR?=	MljvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs	NHrE[XlHfW6ldHnvckBCe3OjeR?=	M2XSN\|EwOy9zMDFOwG0>	MVWxJIg>		NUDFOWU{cW6qaXLpeJMhWzGSLYP0bY12dGG2ZXSgRYt1KHCqb4PwbI9zgWyjdHnvckAhyqB?	NFXwVXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUezOFkxOCd-MkW3N\|Q6ODB:L3G+
HPAEpiCs	MlKwSpVv[3Srb36gRZN{[Xl?	NX[5SpJlOS9\|L{GwJO69VQ>?	MlvBNUBp		M3i3NolvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u	M2XsOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0PVAxLz5{NUezOFkxODxxYU6=
K562	MYnGeY5kfGmxbjDBd5NigQ>?	MknFNVDDqM7:TR?=	NU\LUZZqOcLiaB?=	MnG2SG1UVw>?	M1jqNYlvcGmkaYTzJJF2cW6jY4LpcoUucW6mdXPl[EBxOzhiTVHQT{BxcG:\|cHjvdplt[XSrb36=	NXzsc5VSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFQxPDNpPkK1Olg1ODR\|PD;hQi=>
PANC-1	NFrLbFJHfW6ldHnvckBCe3OjeR?=	M{CwdlExKM7:TR?=	NYnoVIlFOSCq		NVHmXIMz\W6qYX7j[ZMhfGinIHH1eI9xcGGpaXOg[YZn\WO2wrC=	MnjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{KyNlIoRjJ3NkOyNlIzRC:jPh?=
BxPC-3	M{XwZWZ2dmO2aX;uJGF{e2G7	Mn7BNVAh\|ryP	MX[xJIg>		NGLrUlRmdmijbnPld{B1cGViYYX0c5Bp[WerYzDl[oZm[3UEoB?=	NUX5OmppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N\|IzOjJpPkK1OlMzOjJ{PD;hQi=>
K562	M4i1fmZ2dmO2aX;uJGF{e2G7	MX[wMlI2NTFizszN	NXv4VFlWOjRiaB?=		NX;adnNve3WycILld5NmeyC{ZYP2[ZJifHKxbD3pcoR2[2WmIFiyRXgheGixc4Doc5J6dGG2aX;u	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzOUO5Nkc,OjV4MUmzPVI9N2F-
THP-1	NXHIPFMzTnWwY4Tpc44hSXO\|YYm=	NGLVdnA2KML3TR?=	NGWwd5EzKGh?		MkSxd4lodmmoaXPhcpRtgSCjdITlcpVifGW\|IIPlZ5JmfGmxbjDv[kBKVC1zzsGgbY5lfWOnZDDifUAzP0:KQ3jvcEBxdHW\|IF\TUE0yyqB?	MnnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUi2OlEoRjJ3NUm4OlYyRC:jPh?=
WB	MYHGeY5kfGmxbjDBd5NigQ>?	M2jkflIx6oDLzszNxsA>	M37hT\|Mx6oDLbXnu		MUjk[YNz\WG\|ZYOgeIhmKEySUz2gc5IhVFSDLXnu[JVk\WRiSVytOkBidmRiVF7GMe6yyqCycn;keYN1cW:w	M1T0ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUOwOlgzLz5{NUWzNFY5OjxxYU6=
RAW 264.7	NXvqSXZiTnWwY4Tpc44hSXO\|YYm=	M4nvOlExyqEQvF2=	M16ye\|Mx6oDLbXnu		NFnRdWVqdmS3Y3XzJINp[XKjY4Tldol{fGmlII\hZ5VwdGG2aX;uJI9nKE:Fc9Mg	NYPGPVJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{QTlpPkK1OFYyOzl7PD;hQi=>
RAW 264.7	MWrGeY5kfGmxbjDBd5NigQ>?	NHXMOFkyOMLizszN	NYewNolROzEkgJntbY4>		NIrq[INifHSnboXheIV{KHSqZTDl[oZm[3S\|IH;mJG9RTyCxbjDvd5Rmd2OuYYP0JJJmfHKjY4Tpc44>	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUO5PUc,OjV2NkGzPVk9N2F-
HaCaT	NWnZem12TnWwY4Tpc44hSXO\|YYm=	Mn6wOFDDqM7:TR?=	NWC4dVMyOy1{NDDo	NXXGUHNRTE2VTx?=	NVzGPG5{emWmdXPld{B1cGViYXPjeY12dGG2aX;uJI9nKFqRLUG=	NFHxeFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOVQ5PSd-MkW0N\|U1QDV:L3G+
H9c2	NWHKTo9GTnWwY4Tpc44hSXO\|YYm=	NEPKb442OMLizszNxsA>	NUnOPI9jOTJiaB?=		M{PnXpJm\HWlZYOgUGRJKHKnbHXhd4Uh[W6mIF3NVEBtd3O\|	NET5ZXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK0OVgyQCd-MkWyOFU5OTh:L3G+
HSCs	MWPBdI9xfG:\|aYOgRZN{[Xl?	MnXxNlUh\|ryP	NVXW[ZN3OjUEoHi=		MmfQd4lodmmoaXPhcpRtgSCjdITlcpVifGW\|IGTHMYlv\HWlZXSgZYN1cX[jdHXkJGhUS3NiYYDvdJRwe2m\|	M3fZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[xPVUxLz5{NEm2NVk2ODxxYU6=
THP-1	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NF32SGQ4OiCq	M3HSbGROW09?	NXmxZWprUUN3ME20MlfPxE1?	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNUC4O{c,OjR6MUWwPFc9N2F-
MDDCs	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NIfXRnQ4OiCq	MULEUXNQ	MVPJR\|UxOi55zszN	MnXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs	MmLoSpVv[3Srb36gRZN{[Xl?	NYL6OZE4OC1zNTFOwG0>	M3jqb\|Q5KGh?	NVi4Zo1LTE2VTx?=	NE\TW25{fXCycnXzd4V{KEmITj5OtUBidmRiSWCtNVAheHKxZIXjeIlwdsLi	MlXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs	MXHGeY5kfGmxbjDBd5NigQ>?	MUWwMVE2KM7:TR?=	MWC0PEBp	MYLEUXNQ	MkjrbY5pcWKrdIOgUWlRNTGjLDDNTXAuOWJiYX7kJHJCVlSHUzDwdo9lfWO2aX;u	MkPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs	NI\Od4ZHfW6ldHnvckBCe3OjeR?=	NYLrdY1iOTBizszN	NFHme40{NjViaB?=	NFSxWW5FVVOR	NUTodFhJ[myxY3vzJGVDV1ZiR2CsJIJ2fCCwb4SgWnNXKEdibXXkbYF1\WRiZX70dpkhcW62bzDoeY1idiCPRFTDdy=>	NXfjb2VKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
macrophages	NF2yPHBHfW6ldHnvckBCe3OjeR?=	MWqx5qCK\|ryP	Mn\1OE426oDLaB?=		NIDieZhkd22ybHX0[Yx6KGmwaHnibZR{KE2WLVnJTU1qdmS3Y3XkJIFkfGm4YYTpc44hd2ZiTl[t{tpD	MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEi2N\|MoRjJ2OEC4OlM{RC:jPh?=
PDL	NXKwVYExTnWwY4Tpc44hSXO\|YYm=	MUKyNOKh\|ryPwrC=	M2XX[FMxKG2rbh?=	NWjzcpRNTE2VTx?=	NYfwfVU{e2mpbnnmbYNidnSueTDpcohq[mm2czD0bIUhfGWwc3ns[UBnd3KlZT3t[YRq[XSnZDDCUXAuOiCneIDy[ZN{cW:w	NX3aU3RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OlExQDFpPkK0OVYyODhzPD;hQi=>
AGS	NHP0e\|ZHfW6ldHnvckBCe3OjeR?=	NXvvSGdtPSEQvF2=	NGe1[mY{OCCvaX6=		MX7zeZBxemW\|c3XzxsAyNWmwZIXj[YQh[2G\|cHHz[U05KGGwZDDjZZNx[XOnLUOgZYN1cX[jdHnvci=>	NEfEXY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW0O\|g4QCd-MkS1OFc5Pzh:L3G+
H520	NULiR3hZTnWwY4Tpc44hSXO\|YYm=	MmfqNVDDqML3TR?=	MkL6NUBp	NYLtZ\|RVTE2VTx?=	M1XF[IRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV\|MES3OUc,OjR3M{C0O\|U9N2F-
H1703	MVzGeY5kfGmxbjDBd5NigQ>?	MUmxNOKhyrWP	NHfmb3IyKGh?	NWPwOG5wTE2VTx?=	M2KySYRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO=	MoTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{C0O\|UoRjJ2NUOwOFc2RC:jPh?=
H520	NYCyd4JxTnWwY4Tpc44hSXO\|YYm=	Mlu1NVDDqML3TR?=	NYfiWpo{OTJiaB?=	M{TpR2ROW09?	NF\ETldqdmirYnn0d{Bx\W2ndILlfIVlNWWuaXPpeIVlKE2VSEKgdJJwfGWrbjDzeIFjcWyrdIm=	NV3vb\|BiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N\|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703	M1z3TmZ2dmO2aX;uJGF{e2G7	MWWxNOKhyrWP	Mle4NVIhcA>?	MnvMSG1UVw>?	Mn2zbY5pcWKrdIOgdIVu\XS{ZYjl[E1mdGmlaYTl[EBOW0h{IIDyc5RmcW5ic4ThZoltcXS7	MkLDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{C0O\|UoRjJ2NUOwOFc2RC:jPh?=
H520	M1Xhc2Z2dmO2aX;uJGF{e2G7	NHnS[ogyOMLiwsXN	Mo\tOkBp	MmPrSG1UVw>?	MX3zbYdvcW[rY3HueIx6KGmwY4LlZZNmeyC2aHWgcIV3\Wy\|IH;mJJVjcXG3aYTpck1kd26sdXfheIVlKE2VSEKgbY4heGWvZYTy[Zhm\C22cnXheIVlyqClZXzsJIxqdmV?	M3PPR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
H1703	M1m3WmZ2dmO2aX;uJGF{e2G7	MYWxNOKhyrWP	NIHHdng3KGh?	NFX5cphFVVOR	NYHuXGFOe2mpbnnmbYNidnSueTDpcoNz\WG\|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l	NGjvTmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N\|A1PzV:L3G+
MC3T3-E1	Mn3ISpVv[3Srb36gRZN{[Xl?	M{\HVlAvOy9\|L{OwJO69VQ>?	MWCxJIg>		M{jheYF1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nctMg	Mn;vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEK2PVYoRjJ2NUCyOlk3RC:jPh?=
MC3T3-E1	NH\Y[YFHfW6ldHnvckBCe3OjeR?=	M{O0[FMxKM7:TR?=	NHW4OI0yKGh?		MVvheJRmdnWjdHXzJHRPTi4QsT3zeIlufWyjdHXkJJA{QCCPQWDLJJBpd3OyaH;yfYxifGmxbh?=	NUDpN2hXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFI3QTZpPkK0OVAzPjl4PD;hQi=>
HUVECs	MX;GeY5kfGmxbjDBd5NigQ>?	NGnPSJkyOMLiwsXN	MlvXNUBp		NIjhd2xqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDDXGNNOSCycn;keYN1cW:w	NVjmcoNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PFc6PjRpPkK0OFg4QTZ2PD;hQi=>
AGS	M1jhRmZ2dmO2aX;uJGF{e2G7	NIXOXY4yOMLiwsXN	NX:2e3JiOzEEoH3pci=>		MYjpcohq[mm2czDJUE0y\|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>?	MknRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
MKN-45	MnHqSpVv[3Srb36gRZN{[Xl?	MnTENVDDqML3TR?=	MYSzNOKhdWmw		NIDD[mVqdmirYnn0d{BKVC1zzsKtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB\|OB?=	NWCxTZlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
AGS	NHvq[ItHfW6ldHnvckBCe3OjeR?=	NEDUSmoyOMLiwsXN	M{e2bFMxyqCvaX6=		NY\xZYFl[XS2ZX71ZZRmeyCLTD2x{tIucW6mdXPl[EBISSClZXzsJI1q\3KjdHnvckBidmRiaX72ZZNqd25?	NEXtb\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O\|k3QDF:L3G+
MKN-45	NVTjXFZ6TnWwY4Tpc44hSXO\|YYm=	M2TPVVExyqEEtV2=	NWXpfoRlOzEEoH3pci=>		M4L2foF1fGWwdXH0[ZMhUUxvMd8yMYlv\HWlZXSgS2Eh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9v	NEL5VYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O\|k3QDF:L3G+
AGS	MmDQSpVv[3Srb36gRZN{[Xl?	MmLaNVDDqML3TR?=	M4jDcVMxyqCvaX6=		MoPJd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25?	NUnyNVl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45	MVPGeY5kfGmxbjDBd5NigQ>?	NHvHcmYyOMLiwsXN	M2n3N\|MxyqCvaX6=		NFT0Rlh{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDJcE0y\|rJvaX7keYNm\CCPTWCyJIFv\CCPTWC5JI1TVkFiZYjwdoV{e2mxbh?=	NYTaTo81RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|k3QDFpPkK0OFc6PjhzPD;hQi=>
DCs	NFT5PJJHfW6ldHnvckBCe3OjeR?=	M4TxRVIxyqEQvF2=	M3LxUFHDqGh?		NIDUZWNl\WO{ZXHz[ZMhUUxvMUKgdJJw\HWldHnvci=>	NXX5do1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N\|Q3OzZpPkK0OFM1PjN4PD;hQi=>
HUVEC	MmLpSpVv[3Srb36gRZN{[Xl?	NH;4NowzOCEQvH5CpC=>	NGXHdGg2KGh?	NHOydYxFVVOR	NXL6dos4emWmdXPld{BkgXSxa3nu[UBmgHC{ZYPzbY9vKGyndnXsd{BqdiCjIHPvcoNmdnS{YYTpc44u\GWyZX7k[Y51KG2jbn7ldi=>	NV\G[YV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPFkxPjJpPkK0NVg6ODZ{PD;hQi=>
A 549	MXfGeY5kfGmxbjDBd5NigQ>?	MnHGOVAh\|ryP	NYrjXpZqOSCq		MYPk[YNz\WG\|ZYOgeIhmKGyndnXsJI9nKEmOLUlCpC=>	MkThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{m2PFgoRjJ2MUe5Olg5RC:jPh?=
H520	MWjGeY5kfGmxbjDBd5NigQ>?	M2HSTVUwOTBizszN	M{SxfVEhcA>?	NH\WOYxFVVOR	Mm\n[IVkemWjc3XzJG1UUDJicILveIVqdiCjczD3[YxtKGG\|IH3SUmEhdGW4ZXzzJIlvKGenZnn0bY5q[i2neIDvd4VlKGOnbHy=	M4Tkd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
H1703	M1OyPWZ2dmO2aX;uJGF{e2G7	MYi1M\|ExKM7:TR?=	MXixJIg>	MnXJSG1UVw>?	Mlzh[IVkemWjc3XzJG1UUDJicILveIVqdiCjczD3[YxtKGG\|IH3SUmEhdGW4ZXzzJIlvKGenZnn0bY5q[i2neIDvd4VlKGOnbHy=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF\|OEmwN{c,OjRzM{i5NFM9N2F-
H520	NYHhWI1jTnWwY4Tpc44hSXO\|YYm=	MYixNOKhyrWP	MlTkNVIhcA>?	NXTPc5FUTE2VTx?=	NUfldng{\GWlcnXhd4V{KE2VSEKgcXJPSSCjbnSgdJJwfGWrbjDzeIFjcWyrdImgbY4h\2WoaYTpcoljNXS{ZXH0[YQhVlOFTFOgZ4VtdHN?	M3v4N\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
H1703	M17SUGZ2dmO2aX;uJGF{e2G7	MUGxNOKhyrWP	MnvDNVIhcA>?	MnrqSG1UVw>?	Mnfl[IVkemWjc3XzJG1UUDJibWLORUBidmRicILveIVqdiC\|dHHibYxqfHliaX6g[4VncXSrbnniMZRz\WG2ZXSgUnNEVENiY3XscJM>	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF\|OEmwN{c,OjRzM{i5NFM9N2F-
MCF-7	M4XtUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlS1NVDDqM7:TR?=	NIXzS\|QzPMLiaB?=		NWDmflAycW6qaXLpeJMhfGinIFPSNVA5NWmwZIXj[YQh[2WubDDk[YF1cA>?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{OEi1N{c,OjRzMki4OVM9N2F-
HPAEpiCs	NXfMZpVTTnWwY4Tpc44hSXO\|YYm=	M4XTZ\|AvOS9zL{GwJO69VQ>?	M1HOcFEhcA>?		NV[xTFJkcW6qaXLpeJMhXE6ILd8xMYlv\HWlZXSgZ3BNSTMEoIDyc5RmcW5iYX7kJI1TVkFiZYjwdoV{e2mxbh?=	NFHCcnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2PVE2QCd-MkSwOlkyPTh:L3G+
podocytes	NWL0eFFlTnWwY4Tpc44hSXO\|YYm=	M1nKXFExyqEQvF2=	M{XiN\|EhcA>?		MUfpcohq[mm2czDUS2bPujFvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFugZY5lKEW{a{GvNuKh	NIOwSpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECzOlIyOid-MkSwN\|YzOTJ:L3G+
MCF-7	NInHS5FHfW6ldHnvckBCe3OjeR?=	MkC1NVDDqM7:TR?=	M3nEPVEhcA>?	M2n5T2ROW09?	M{HPZZJm\HWlZYOgeIhmKFeDLXnu[JVk\WRicHjvd5Bpd3K7bHH0[YQheDN6IF3BVGs>	Mo\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUmwPVAoRjJ2MEG5NFkxRC:jPh?=
MCF-7	M3y1fWZ2dmO2aX;uJGF{e2G7	MmrqNVDDqM7:TR?=	M2THNVI1KGh?	NEPMUm1FVVOR	NGq4eJNqdmO{ZXHz[ZMhfGinIGfBMYlv\HWlZXSgZZBweHSxc3nz	NV7qWYIxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkxQTBpPkK0NFE6ODlyPD;hQi=>
HAPI	M{PpXWZ2dmO2aX;uJGF{e2G7	M2S3ZVExNzJyL{SwxsDPxE1?	Mn3kNUBp		NGTSZ2dqdmirYnn0d{BVS0SGLXnu[JVk\WRicEO4M2pPUyCPQWDLJJBpd3OyaH;yfYxifGmxbh?=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OUGyNEc,OjN7NkmxNlA9N2F-
HAPI	MnPwSpVv[3Srb36gRZN{[Xl?	NHvadGUzOMLizszNxsA>	M1LvWVEhcA>?	NGDvfG5FVVOR	NYLqS4JD[XS2ZX71ZZRmeyCWQ1TEMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCrTl;TJIFv\CCycn;keYN1cW:w	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OUGyNEc,OjN7NkmxNlA9N2F-
HepG2	NIHvZ3VHfW6ldHnvckBCe3OjeR?=	MUGzOVAhdk1?	NFf2XpozPCCq		NInVfGVqdmirYnn0d{B1cGViZHXneYVtcW5vaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFu=	M3PzSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUOzNVk5Lz5{M{mzN\|E6QDxxYU6=
AGS	M3XQO2Z2dmO2aX;uJGF{e2G7	MnvENVDDqM7:TR?=	NYjqVHNrOzBibXnu		MX;pcohq[mm2czDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiaX7obYJqfGmxbjDv[kBGWkt?	NYHUW3hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OVA6QTRpPkKzPFUxQTl2PD;hQi=>
HepG2	M4jURmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MkXDNE02OCEQvF2=	Mlj1OFghcA>?		NIflXlRqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=>	M1v6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEC3OVA5Lz5{M{iwO\|UxQDxxYU6=
BEL7404	M{K1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mnv3NE02OCEQvF2=	MmntOFghcA>?		NHLvTGlqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=>	NIjm[IQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO\|UxQCd-MkO4NFc2ODh:L3G+
HL7702	NF:4SlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NUP6N3BlOC13MDFOwG0>	NInlZ3A1QCCq		NYXISVhucW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI>	M{TJbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OEC3OVA5Lz5{M{iwO\|UxQDxxYU6=
HepG2	NF7Od3dHfW6ldHnvckBCe3OjeR?=	NGH1[VUxNTVyIN88US=>	NYL0VHZ5OjRiaB?=		MVrpcohq[mm2czD0bIUheGixc4Doc5J6dGG2aX;uJI9nKHB\|ODDkc5dve3S{ZXHtJJBzd3SnaX7zJG1CWEuDUFuyMEBCXEZ{LDDNV2syKGGwZDDIV3AzPyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>?	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyN{WwPEc,OjN6MEe1NFg9N2F-
BEAS-2B	M3T3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4jW[\|ExKM7:TR?=	MoGyN\|AhdWmw		NYXWdmtZemW4ZYLz[ZMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgTGNK	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN\|Q9N2F-
BEAS-2B	NVy0[XZSS3m2b4TvfIl1gSCDc4PhfS=>	M{TlfFExKM7:TR?=	MV[zNEBucW5?		Ml3lbY5pcWKrdHXkJJRp\SCrbnPy[YF{\SCrbjDMSGgh[W6mIFnMMVgh\XiycnXzd4lwdg>?	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN\|Q9N2F-
BEAS-2B	MUDGeY5kfGmxbjDBd5NigQ>?	MnnFNVAh\|ryP	MVuzNEBucW5?		NFjQZWFl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDjZZNx[XOnLUOsJGJi\CCjbnSg[oF{	NXLObVl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PFQxOzRpPkKzO\|g1ODN2PD;hQi=>
H9c2	M2i5bmZ2dmO2aX;uJGF{e2G7	NYfj[5hiOC5yMT:wMlEwOSEQvF2=	M32xcFEhcA>?		MXzheJRmdnWjdHXzJHRPTi4QsT3pcoR2[2WmIF3NVE06KGW6cILld5Nqd25uIH3SUmEhdGW4ZXzzMEBidmRicILvcY91\XJiYXP0bZZqfHl?	MnnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{SyOVIoRjJ\|N{e0NlUzRC:jPh?=
H9c2	NFrkS2RHfW6ldHnvckBCe3OjeR?=	NYL0e45HOSEQvF2=	M1rmcFEhcA>?		MkPXdoVlfWOnczDUUmYu\|rFiZHny[YN1dHlic4TpcZVt[XSnZDDwN\|ghVUGSSzDwbI9{eGixconsZZRqd25?	M33FU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{e0NlUzLz5{M{e3OFI2OjxxYU6=
U937	NFrVeJpHfW6ldHnvckBCe3OjeR?=	NEX4O20yOMLizszNxsA>	NXXWRpZPOSCq		M4jsc4Fjem:pYYTld{B1cGViY3Hm[oVqdmViZX\m[YN1KG:wIF3LVE0yKGGwZDDQVFJC[87zIH3SUmEhfHKjboPjdolxfGmxbnHsJIxmfmWucx?=	M2DwZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{C3N\|g4Lz5{M{ewO\|M5PzxxYU6=
U937	MX\GeY5kfGmxbjDBd5NigQ>?	M3\LXVExyqEQvF5CpC=>	NH\nR2UyKGh?		MVfhZpJw\2G2ZYOgZ4Fn\mWrbnWtbY5lfWOnZDDNT3AuOSCmb4fuMZJm\3WuYYTpc44h[W6mIGDQNmFk\|rFidYCtdoVofWyjdHnvci=>	NIn4N5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO\|M5Pyd-MkO3NFc{QDd:L3G+
U937	MkXaSpVv[3Srb36gRZN{[Xl?	NXvSU4cyOTEEoN88UeKh	NWLuR4x[OSCq		M3;BfZN2eHC{ZYPz[ZMh[y2MdX6gZY5lKEOURVKgdIhwe3Cqb4L5cIF1cW:wIHnuJINi\m[naX7lMZRz\WG2ZXSgZ4VtdHQEoB?=	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{O4O{c,OjN5MEezPFc9N2F-
A549	NUHQ[5hKTnWwY4Tpc44hSXO\|YYm=	Mn:5NE4{NzNxM{Cg{txO	MUWxJIg>		NYDNUGY6e2mpbnnmbYNidnSueTDheJRmdnWjdHXzJGFVWM7\|Uz3t[YRq[XSnZDDDU3guOiCycn;0[YlvKGGwZDDtVm5CKGW6cILld5Nqd25iYX7kJJBzd22xdHXyJIFkfGm4aYT5xsA>	NV3jdWk2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549	M4LmNWZ2dmO2aX;uJGF{e2G7	Mn[2NVDDqM7:TdMg	M2Dp[VAuOzBibXnu		M{DLZolvcGmkaYTzJGFVWM7\|UzDpcoR2[2WmIIC0Nk9xPDRiTVHQT{BidmRicEO4JG1CWEticHjvd5Bpd3K7bHH0bY9v	NV64Xoo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549	MnjrSpVv[3Srb36gRZN{[Xl?	NUP2ZnlLOTEEoN88UeKh	M2jxWFEhcA>?		NHO3ZmNqdmirYnn0d{BCXFEQs2OgbY5lfWOnZDDOSk3PwkJicE[1JJN2[nWwaYSgdIhwe3Cqb4L5cIF1cW:wIHHu[EBPTi4QulKgdJJwdW:2ZYKgZYN1cX[rdIm=	Ml\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEC2O\|QoRjJ\|NkiwOlc1RC:jPh?=
A549	M2H0[GZ2dmO2aX;uJGF{e2G7	MYexNOKh\|ryPwrC=	M4HpblEhcA>?		M1f5O5Jm\HWlZYOgRXRR\|rOVLYP0bY12dGG2ZXSgZ3BNSTJicHjvd5Bpd3K7bHH0bY9vyqB?	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6ME[3OEc,OjN4OEC2O\|Q9N2F-
A549	NVnOSGZWTnWwY4Tpc44hSXO\|YYm=	NFnZXogyOMLizszNxsA>	NYTSd4w4OSCq		NFLKVY1z\WS3Y3XzJGFVWM7\|Uz3lcohidmOnZDDlcpp6dWG2aXOgZYN1cX[rdImgc4Yh[1COQUNCpC=>	NUXGRoFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
PC12	M37WdWZ2dmO2aX;uJGF{e2G7	MX:xNE8zOC92MDFOwG0>	NITxZXUyyqCq		Mm[zbY5pcWKrdIOgTm5MKGGwZDDwN\|g>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV6NEO1O{c,OjN3OESzOVc9N2F-
HK-2	MUnBdI9xfG:\|aYOgRZN{[Xl?	NHHWeVAzOMLizszN	NEjYZVEzPCCq		NH\3d4VqdmirYnn0d{BGWktiYX7kJJA{QE2DUFu=	MkHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEOxOVEoRjJ\|NUSzNVUyRC:jPh?=
H9c2	NHzxNFVHfW6ldHnvckBCe3OjeR?=	M{G0ZVHDqM7:TR?=	NWj3RVRvOSCq		NHLUPJVz\WS3Y3XzJHRPTi4QsT3pcoR2[2WmIF3NVE06KG2UTlGgcIV3\Wy\|IHHu[EBxem:vb4TldkBi[3Srdnn0fS=>	M4PQUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{WzOlk6Lz5{M{O1N\|Y6QTxxYU6=
H9c2	NIPBZnRHfW6ldHnvckBCe3OjeR?=	NXXpVolJOcLizszN	NYizU4RbOSCq		M4rvb5Jm\HWlZYOgWG5HNc7zLXXubIFv[2WmIFHQMVEheHKxbX;0[ZIh[WO2aY\peJk>	MnTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NUO2PVkoRjJ\|M{WzOlk6RC:jPh?=
H1650	MV3GeY5kfGmxbjDBd5NigQ>?	NVnWU3dsOTEEoN88UeKh	MXexJIg>	MVTEUXNQ	M1fnS4Rm[3KnYYPld{Bjd3SqIIDyc5RmcW5iYX7kJI1TVkFibHX2[Yx{KG:oIFXSR2MyKGmwIIDhZ4xqfGG6ZXyt[Zhxd3OnZNMgZ4VtdHN?	M4\0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkK4Olk3Lz5{M{KyPFY6PjxxYU6=
H1703	NVTZWphvTnWwY4Tpc44hSXO\|YYm=	MXuxNOKh\|ryPwrC=	NIfEemwyKGh?	MkXUSG1UVw>?	NGPUSYNl\WO{ZXHz[ZMh[m:2aDDwdo91\WmwIHHu[EBuWk6DIHzleoVteyCxZjDFVmNEOSCrbjDwZYNtcXSjeHXsMYV5eG:\|ZXVCpINmdGy\|	NFfMZo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KyPFY6Pid-MkOyNlg3QTZ:L3G+
H1650	NVfZb5l4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M4q3e\|ExyqEQvF5CpC=>	MWOxJIg>	NFXYXWVFVVOR	MlHO[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>?	M2\UWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkK4Olk3Lz5{M{KyPFY6PjxxYU6=
H1703	NGrLZ\|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUexNOKh\|ryPwrC=	M3LCcFEhcA>?	NGPPWm5FVVOR	M13oUYVvcGGwY3XzJJBi[2yrdHH4[YwucW6mdXPl[EBkgXSxdH;4bYNqfHl?	MlTUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{Mki2PVYoRjJ\|MkK4Olk3RC:jPh?=
medulloblastoma cells	MYLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				M4rMXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUA{NjByNjFOwG0v	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N\|E9N2F-
neural precursor cells	NIPV[pdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkhVVSWIHHzd4F6NCCHQ{WwJF0hQC5yNkOg{txONg>?	MmrTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N\|EoRjF5NEG3OlMyRC:jPh?=
RAW264.7	NUK0NoZjSW62aXnu[oxidW2jdH;yfUBie3OjeR?=		MUexNEBucW6\|		NE[4WXRCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbnP1ZoF1\WRiZn;yJFExKG2rboOgdJJqd3JidH:gUHBUNWOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAyQCCqcoOgZpkhT3KrZYPzJI1mfGixZDygTWM2OCB;IEG2JO69VS5?	MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{G3PVgoRjJ{OEOxO\|k5RC:jPh?=
BL21(DE3)	NF3vW4hHfW6ldHnvckBie3OjeR?=		MW[2NEBucW6\|		MljzTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCQLYTldo1qdmGuIFjpd\|YufGGpZ3XkJGJTTDRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSiGRUOpJINmdGy\|IIXzbY5oKGKrb4Tpcplt[XSnZDDobZN1d26nIFi0JJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkhSWyyaHHTZ5Jm\W5iYYPzZZktKEmFNUCgQUA{NjRizszNMi=>	M1jueFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUm1O\|I{Lz5{OEG5OVczOzxxYU6=
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2 ; pJNK1 / pJNK2 / JNK1 / JNK2	26844273 18222647
Immunofluorescence	p-p38 / MMP9	24479681
Growth inhibition assay	Cell viability	26844273

In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. ^[5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. ^[8]

Protocol (from reference)

Kinase Assay: ^[1]	In vitro kinase assays: The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-³³P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-³³P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.
Cell Research: ^[2]	Cell lines: Jurkat, and HeLa Concentrations: Dissolved in DMSO, final concentrations ~50 μM Incubation Time: 24 hours Method: Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.
Animal Research: ^[5]	Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG Dosages: 12.5 μg Administration: Administered via i.d.

References

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Solubility (25°C)

In vitro


In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 5%DMSO+40%PEG300+5%Tween80+50%H2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	8 mg/mL

Chemical Information

Molecular Weight	331.34
Formula	C₂₀H₁₄N₃OF
CAS No.	152121-30-7
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

Download SB202190 (FHPI) SDF

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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