SB202190 (FHPI)

For research use only.

Catalog No.S1077

118 publications

SB202190 (FHPI) Chemical Structure

CAS No. 152121-30-7

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

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Selleck's SB202190 (FHPI) has been cited by 118 publications

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Choose Selective p38 MAPK Inhibitors

Biological Activity

Description SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.
Targets
Ferroptosis [11]
()
p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. [2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. [3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). [4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. [6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 NHLFRVBHfW6ldHnvckBCe3OjeR?= NYfvcZc3OjVizszN NEf0NIg{OCCvaX6= Ml3KSG1UV8Li MoSzZZR1\W63YYTld{B1cGVibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:wIH;mJIhDTC1{IHnuJJJme3CxboPpeoUhfG9iRFG= NGHYVVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkKyN|I2OSd-Mk[yNlMzPTF:L3G+
MDA-MB-231 MnnXSpVv[3Srb36gRZN{[Xl? Mlf2NkDPxE1? NHztWXUzPCCq MYDs[ZN{\W6nczDDR2wzKGmwZIXjeIlwdiCkeTDUUmbPuQ>? MoLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzMEC4OFgoRjJ4MUCwPFQ5RC:jPh?=
rBMSCs NILhVFhHfW6ldHnvckBCe3OjeR?= MUexNEDPxE1? NFT5U3MzNjViaB?= NUPGbpRo\GWycnXzd4V{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSXJMKGGwZDDwN|g> M3XYWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEWzNlY3Lz5{NkC1N|I3PjxxYU6=
MG63 M4jtR2Z2dmO2aX;uJGF{e2G7 MV:xNE8zOC9|MDFOwG0> M2nJTFI1KGh? NX3LfHl[e2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgeIhmKGyndnXsJI9nKHCqb4PwbI9zgWyjdHXkJJA{QCCrbnT1Z4VlKGK7IFPkR4wzKGmwIHGgZ49v[2WwdILheIlwdiBvZHXw[Y5l\W62IH3hco5meg>? NGTE[mU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm5PFMyOid-MkW5PVg{OTJ:L3G+
MG63 NYfZVW1RSXCxcITvd4l{KEG|c3H5 MnriNVAwOjBxM{Cg{txO NU[xOZo1OjRiaB?= MXvzbYdvcW[rY3HueIx6KGSnY4LlZZNm\CC2aHWgZZBweHSxc3nzJJJifGVib3[gUWc3OyCrbnT1Z4VlKGK7IFPkR4wz MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl7OEOxNkc,OjV7OUizNVI9N2F-
HTSMCs NY\Q[HZvTnWwY4Tpc44hSXO|YYm= MnrxNE4yNzFxMUCg{txO MVuxJIg> NUT1SWlKcW6qaXLpeIVlKEORUl2tNk1qdmS3Y3XkJGhQNTFicILveIVqdiCuZY\lcJMh[W6mIH3SUmEh\XiycnXzd4lwdg>? NVnBNGN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5NlE1PjRpPkK1PVIyPDZ2PD;hQi=>
MIA PaCa-2 M2jnO2Z2dmO2aX;uJGF{e2G7 NFPPcGYzOMLizszN M3Hjb|I1KGh? NHvuR2hz\WS3Y3XkJIxi[3SjdHWgZYNkfW23bHH0bY9vKGmwIHPvcYJqdmG2aX;uJJdqfGhiYn;0bEAzNUSJIHHu[EBFNWGubH;z[eKh M2rlN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
MIA PaCa-2 NEjyeHdHfW6ldHnvckBCe3OjeR?= NIL1fJMzOMLizszN NF7ISmMzPCCq MXTy[ZN2dHS|IHnuJIEhdW:mZYP0JIlvcGmkaYTpc44hd2ZiSFnGMVHPuSCycn;0[YlvKGGlY4XteYxifGmxbh?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
BxPC-3 M3:wU2Z2dmO2aX;uJGF{e2G7 Mlj6NlDDqM7:TR?= NGPoUGgzPCCq NVXENlM4emW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> NH75NGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
AsPC-1 NXS2Sm5qTnWwY4Tpc44hSXO|YYm= NH7OVZgzOMLizszN MmrhNlQhcA>? NVXFfplDemW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> NH3vRmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2 M2DxOGZ2dmO2aX;uJGF{e2G7 NFHV[pMzOMLizszN NFrzN5YzPCCq NHfJfmNmdmijbnPld{BkdGWjdnHn[UBw\iCSQWLQJJdp\W5iY3;tZolv\WRid3n0bEBodHWlb4PlJIFv[WyxZ4O= NEfPSYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2 M{DsNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnPSoNPOjEEoN88US=> M1nnVlIhcA>? NHu4cpl{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? NXzHcFhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
BxPC-3 M372S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;TfndEOjEEoN88US=> NYrVfYNFOiCq MUXz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> Mn:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
AsPC-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk[xNlDDqM7:TR?= MYiyJIg> NXLiSYp3e2Wwc3n0bZpmeyClZXzsJIxqdmW|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On NFi3T5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
HEY MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLpWHAzOMLizszN M3jod|IhcA>? MkO3d4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm NYrWSlkzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
OVCAR-3 M3e0PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXKyNOKh|ryP M1;rPVIhcA>? NELa[YR{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? MnOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
SK-OV-3 MkDtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzJNlDDqM7:TR?= NXPTVlRpOiCq NHv1eVJ{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
MH7A  NXfiT29JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37xSFI1KGh? NVzJTHlOemWrbn\vdoNmeyC2aHWgbY5pcWKrdH;yfUBm\m[nY4TzJI9nKFiDTh?= NGDuO4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2Nlk3Pid-MkW4OlI6PjZ:L3G+
MH7A  MljyRZBweHSxc3nzJGF{e2G7 NELvVnozPSEQvF2= M1u0clI1KGh? MYPy[ZZmenOnczDj[YxtKGO7Y3zlJIFzemW|dDDpcoR2[2WmIHL5JHhCViCjbnSgZ4F2e2WmIHHwc5B1d3OrczDv[kBk\WyuczD2bYEh[WO2aY\heIlwdiCxZjDKUms> NH3iSHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2Nlk3Pid-MkW4OlI6PjZ:L3G+
SCC25 NGPKe4NHfW6ldHnvckBCe3OjeR?= MmXlNlAh|ryP M3Puc|I1KGh? MlHmbY5kemWjc3XzJIF2fG:yaHHnfUBt\X[nbB?= M1XVV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAxLz5{NUizOFQxODxxYU6=
HaCaT  MUfGeY5kfGmxbjDBd5NigQ>? M4H0flUhyrWP M{S0XVI1KGh? NFjiR4lqdmirYnn0d{BKTk5vzsOtbY5lfWOnZDDDR2wzOiCycn;keYN1cW:wIHzleoVtew>? M4\vcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0N|U{Lz5{NUizOFM2OzxxYU6=
HaCaT  NIrCeWNHfW6ldHnvckBCe3OjeR?= MXq1JOK2VQ>? M2q3SVI1KGh? MUTpcohq[mm2czDJSm4u|rFvaX7keYNm\CCFQ1yyNkBxem:mdXP0bY9vKGyndnXsdy=> M4\qbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0N|U{Lz5{NUizOFM2OzxxYU6=
HPAEpiCs  NXPGe|dkTnWwY4Tpc44hSXO|YYm= NH32[ngyNzNxMUCg{txO M4XkPFEhcA>? MVvy[YR2[2W|IGOxVE1qdmS3Y3XkJGlESU1vMTDwdo91\WmwIHHu[EBuWk6DIHX4dJJme3Orb36gZY5lKHC{b33veIVzKGGldHn2bZR6 MmXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs MnvMSpVv[3Srb36gRZN{[Xl? MY[xM|MwOTBizszN M3jEdVEhcA>? MUXpcohq[mm2czDTNXAue3SrbYXsZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:wIDFCpC=> M2jXXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0PVAxLz5{NUezOFkxODxxYU6=
HPAEpiCs NGXpUWZHfW6ldHnvckBCe3OjeR?= Ml\vNU8{NzFyIN88US=> NGn1WYgyKGh? M3r4S4lvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u NVLrT5hSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3N|Q6ODBpPkK1O|M1QTByPD;hQi=>
K562 NV:wSnhxTnWwY4Tpc44hSXO|YYm= NH;icGIyOMLizszN NHzsb3UyyqCq Ml;pSG1UVw>? M3zzXYlvcGmkaYTzJJF2cW6jY4LpcoUucW6mdXPl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36= NHPEfHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4OFA1Oyd-MkW2PFQxPDN:L3G+
PANC-1 NF3QeWRHfW6ldHnvckBCe3OjeR?= NV3qRpRYOTBizszN NILYe4IyKGh? MXrlcohidmOnczD0bIUh[XW2b4DoZYdq[yCnZn\lZ5TDqA>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ|MkKyNkc,OjV4M{KyNlI9N2F-
BxPC-3 NIPNXGxHfW6ldHnvckBCe3OjeR?= MWOxNEDPxE1? NETzeZMyKGh? M3zvUIVvcGGwY3XzJJRp\SCjdYTvdIhi\2mlIHXm[oVkfMLi NVjsO4FlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|IzOjJpPkK1OlMzOjJ{PD;hQi=>
K562  NUP6PHR3TnWwY4Tpc44hSXO|YYm= MXmwMlI2NTFizszN M163[|I1KGh? NVnadZJve3WycILld5NmeyC{ZYP2[ZJifHKxbD3pcoR2[2WmIFiyRXgheGixc4Doc5J6dGG2aX;u MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzOUO5Nkc,OjV4MUmzPVI9N2F-
THP-1 M1y3c2Z2dmO2aX;uJGF{e2G7 MorkOUDDvU1? NYjUUFdvOiCq NY[wO3lFe2mpbnnmbYNidnSueTDheJRmdnWjdHXzJJNm[3KndHnvckBw\iCLTD2x{tEhcW6mdXPl[EBjgSB{N1;IR4hwdCCybIXzJGZUVC1zwrC= Mli3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUi2OlEoRjJ3NUm4OlYyRC:jPh?=
WB NXPqNpR7TnWwY4Tpc44hSXO|YYm= M3vBbFIx6oDLzszNxsA> MXWzNQKBkW2rbh?= NULRN3pl\GWlcnXhd4V{KHSqZTDMVHMuKG:{IFzURU1qdmS3Y3XkJGlNNTZiYX7kJHRPTi4QsdMgdJJw\HWldHnvci=> MnP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3M{C2PFIoRjJ3NUOwOlgzRC:jPh?=
RAW 264.7 M{jaOWZ2dmO2aX;uJGF{e2G7 NXT1NYJFOTEEoN88US=> MlvkN|DjiImvaX6= M1OyU4lv\HWlZYOgZ4hiemGldHXybZN1cWNidnHjeY9t[XSrb36gc4YhV0O|wrC= NF7oNWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NVM6QSd-MkW0OlE{QTl:L3G+
RAW 264.7 M3XpXWZ2dmO2aX;uJGF{e2G7 MYWxNOKh|ryP MUWzNQKBkW2rbh?= NGjlSmVifHSnboXheIV{KHSqZTDl[oZm[3S|IH;mJG9RTyCxbjDvd5Rmd2OuYYP0JJJmfHKjY4Tpc44> M4\1OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NE[xN|k6Lz5{NUS2NVM6QTxxYU6=
HaCaT  M1rmZmZ2dmO2aX;uJGF{e2G7 MmK2OFDDqM7:TR?= NHq1UmI{NTJ2IHi= M123RmROW09? M1LsRZJm\HWlZYOgeIhmKGGlY4XteYxifGmxbjDv[kBbVy1z NHPxWVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOVQ5PSd-MkW0N|U1QDV:L3G+
H9c2 MnHISpVv[3Srb36gRZN{[Xl? MoTZOVDDqM7:TdMg NWjZNph2OTJiaB?= NW[1cWw{emWmdXPld{BNTEhicnXs[YF{\SCjbnSgUW1RKGyxc4O= M4f6R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkS1PFE5Lz5{NUK0OVgyQDxxYU6=
HSCs MXXBdI9xfG:|aYOgRZN{[Xl? MUeyOUDPxE1? M1n1VFI1yqCq NVnz[lEye2mpbnnmbYNidnSueTDheJRmdnWjdHXzJHRINWmwZIXj[YQh[WO2aY\heIVlKEiVQ4OgZZBweHSxc3nz M1LPblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[xPVUxLz5{NEm2NVk2ODxxYU6=
THP-1 M4fHZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXaO|IhcA>? NEPmb5BFVVOR M4HQW2lEPTB;ND63{txO Mlv3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs M3izbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPEPYd5PzJiaB?= MUTEUXNQ M3XiN2lEPTB{LkhOwG0> NYK5cphnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs NUjjVmZwTnWwY4Tpc44hSXO|YYm= MmTpNE0yPSEQvF2= M{K4fVQ5KGh? M1K0cWROW09? MULzeZBxemW|c3XzJGlHVi4QsTDhcoQhUVBvMUCgdJJw\HWldHnvcuKh NUntR2g4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs MWLGeY5kfGmxbjDBd5NigQ>? MUCwMVE2KM7:TR?= MVO0PEBp M1XFXGROW09? MlnzbY5pcWKrdIOgUWlRNTGjLDDNTXAuOWJiYX7kJHJCVlSHUzDwdo9lfWO2aX;u MoS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs M2TXNWZ2dmO2aX;uJGF{e2G7 M3q5ZVExKM7:TR?= M3:1OlMvPSCq MVXEUXNQ NFvXblRjdG:la4OgSWJQXiCJUDygZpV1KG6xdDDWV3YhTyCvZXTpZZRm\CCnboTyfUBqdnSxIHj1cYFvKE2GRFPz MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNUC4O{c,OjR6MUWwPFc9N2F-
macrophages NHPofotHfW6ldHnvckBCe3OjeR?= NH\CTHIy6oDLzszN NIS4ZXk1NjYkgJno MULjc41xdGW2ZXz5JIlvcGmkaYTzJG1VNUmLST3pcoR2[2WmIHHjeIl3[XSrb36gc4YhVkZvzsrC MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhyOE[zN{c,OjR6MEi2N|M9N2F-
PDL  MmHzSpVv[3Srb36gRZN{[Xl? NXT4W3ZGOjEEoN88UeKh MmH5N|AhdWmw MoXLSG1UVw>? MmTQd4lodmmoaXPhcpRtgSCrbnjpZol1eyC2aHWgeIVve2muZTDmc5Jk\S2vZXTpZZRm\CCETWCtNkBmgHC{ZYPzbY9v NXK2WpNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OlExQDFpPkK0OVYyODhzPD;hQi=>
AGS M4DEcGZ2dmO2aX;uJGF{e2G7 NGexU4E2KM7:TR?= MUWzNEBucW5? MWjzeZBxemW|c3XzxsAyNWmwZIXj[YQh[2G|cHHz[U05KGGwZDDjZZNx[XOnLUOgZYN1cX[jdHnvci=> M3LjSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUS3PFc5Lz5{NEW0O|g4QDxxYU6=
H520 MnTtSpVv[3Srb36gRZN{[Xl? NFvpR4kyOMLiwsXN NXTu[opTOSCq NWGwbmFbTE2VTx?= MXjk[YNz\WG|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy| MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV|MES3OUc,OjR3M{C0O|U9N2F-
H1703 M{nkbGZ2dmO2aX;uJGF{e2G7 MmT6NVDDqML3TR?= NHj2fYEyKGh? M2XOOWROW09? MVrk[YNz\WG|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy| MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV|MES3OUc,OjR3M{C0O|U9N2F-
H520 NYrEVWcyTnWwY4Tpc44hSXO|YYm= Mme0NVDDqML3TR?= MUexNkBp M1rtVmROW09? MYfpcohq[mm2czDw[Y1mfHKneHXkMYVtcWOrdHXkJG1UUDJicILveIVqdiC|dHHibYxqfHl? NIHVcW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N|A1PzV:L3G+
H1703 MXnGeY5kfGmxbjDBd5NigQ>? MUixNOKhyrWP NXXhZ3ZoOTJiaB?= MlfOSG1UVw>? MmjobY5pcWKrdIOgdIVu\XS{ZYjl[E1mdGmlaYTl[EBOW0h{IIDyc5RmcW5ic4ThZoltcXS7 NGLuU|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N|A1PzV:L3G+
H520 NYXYeIQ2TnWwY4Tpc44hSXO|YYm= MkLtNVDDqML3TR?= NXfjR|VTPiCq MoThSG1UVw>? NULJXIVCe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l NVW5PGp2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703 NFHqXHZHfW6ldHnvckBCe3OjeR?= NHHHOIkyOMLiwsXN NH;2cWg3KGh? M16xW2ROW09? Mn\Vd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNidHjlJIxmfmWuczDv[kB2[mmzdXn0bY4u[2:waoXnZZRm\CCPU1iyJIlvKHCnbXX0doV5\WRvdILlZZRm\MLiY3XscEBtcW6n M3HSWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
MC3T3-E1 NYe5V4puTnWwY4Tpc44hSXO|YYm= MXqwMlMwOy9|MDFOwG0> M{O4elEhcA>? MY\heJRmdnWjdHXzJHRPTi4QsT3pcoR2[2WmIF3NVE06KGW6cILld5Nqd25iaX6gZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDtZY5v\XMEoB?= MknPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEK2PVYoRjJ2NUCyOlk3RC:jPh?=
MC3T3-E1 M3vRbGZ2dmO2aX;uJGF{e2G7 NEjlZ|M{OCEQvF2= MYWxJIg> NVnycoZX[XS2ZX71ZZRmeyCWTl[t{tEue3SrbYXsZZRm\CCyM{igUWFRUyCyaH;zdIhwenmuYYTpc44> NXi1dmVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFI3QTZpPkK0OVAzPjl4PD;hQi=>
HUVECs  NF;xOnhHfW6ldHnvckBCe3OjeR?= M2HuWVExyqEEtV2= M1fBNVEhcA>? MkfVbY5pcWKrdIOgWG5HNc7zLXnu[JVk\WRiQ2jDUFEheHKxZIXjeIlwdg>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR6N{m2OEc,OjR2OEe5OlQ9N2F-
AGS  MXnGeY5kfGmxbjDBd5NigQ>? NYfvfG5MOTEEoNM1US=> NF3USlk{OMLibXnu M4i2SYlvcGmkaYTzJGlNNTIQsj3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6 MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5OU[4NUc,OjR2N{m2PFE9N2F-
MKN-45 NGfZSGRHfW6ldHnvckBCe3OjeR?= NFrZfo4yOMLiwsXN MYWzNOKhdWmw MWnpcohq[mm2czDJUE0y|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>? NWfkV2tNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
AGS  M4HC[WZ2dmO2aX;uJGF{e2G7 Mm\jNVDDqML3TR?= MWWzNOKhdWmw MUfheJRmdnWjdHXzJGlNNTIQsj3pcoR2[2WmIFfBJINmdGxibXnndoF1cW:wIHHu[EBqdn[jc3nvci=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5OU[4NUc,OjR2N{m2PFE9N2F-
MKN-45 MYLGeY5kfGmxbjDBd5NigQ>? MUOxNOKhyrWP MUizNOKhdWmw NIfmcmdifHSnboXheIV{KEmOLUJOtk1qdmS3Y3XkJGdCKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdg>? M2DJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEe5OlgyLz5{NES3PVY5OTxxYU6=
AGS  MnTLSpVv[3Srb36gRZN{[Xl? MkXrNVDDqML3TR?= NW\BdFFDOzEEoH3pci=> NF\CV2F{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDJcE0y|rJvaX7keYNm\CCPTWCyJIFv\CCPTWC5JI1TVkFiZYjwdoV{e2mxbh?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5OU[4NUc,OjR2N{m2PFE9N2F-
MKN-45 MXTGeY5kfGmxbjDBd5NigQ>? NXHR[o9lOTEEoNM1US=> M1KxbVMxyqCvaX6= NVK1W5I{e2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgTYwuOc7{LXnu[JVk\WRiTV3QNkBidmRiTV3QPUBuWk6DIHX4dJJme3Orb36= NHHDN3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O|k3QDF:L3G+
DCs M1nFfmZ2dmO2aX;uJGF{e2G7 NGjjOGgzOMLizszN NF3COY8yyqCq NH7wWlhl\WO{ZXHz[ZMhUUxvMUKgdJJw\HWldHnvci=> NF3sVYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESzOFY{Pid-MkS0N|Q3OzZ:L3G+
HUVEC  NHq3TW9HfW6ldHnvckBCe3OjeR?= M3rHSFIxKM7:bdMg MnXqOUBp MnrwSG1UVw>? NGHYXJdz\WS3Y3XzJIN6fG:taX7lJIV5eHKnc4Ppc44hdGW4ZXzzJIlvKGFiY3;uZ4VvfHKjdHnvck1l\XCnbnTlcpQhdWGwbnXy MnjOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEmwOlIoRjJ2MUi5NFYzRC:jPh?=
A 549 NV\WTIROTnWwY4Tpc44hSXO|YYm= Ml\oOVAh|ryP Mn;DNUBp M{TpdoRm[3KnYYPld{B1cGVibHX2[Ywhd2ZiSVytPOKh MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5OU[4PEc,OjRzN{m2PFg9N2F-
H520  MmnuSpVv[3Srb36gRZN{[Xl? NF7VXoc2NzFyIN88US=> MmHZNUBp NYjB[nRwTE2VTx?= MXvk[YNz\WG|ZYOgUXNJOiCycn;0[YlvKGG|IIflcIwh[XNibWLORUBt\X[nbIOgbY4h\2WoaYTpcoljNWW6cH;z[YQh[2WubB?= NUTvdWN2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|g6ODNpPkK0NVM5QTB|PD;hQi=>
H1703  NIO0RoZHfW6ldHnvckBCe3OjeR?= NUe3RpNMPS9zMDFOwG0> MYCxJIg> MmPQSG1UVw>? M{PISYRm[3KnYYPld{BOW0h{IIDyc5RmcW5iYYOge4VtdCCjczDtVm5CKGyndnXsd{BqdiCpZX\peIlvcWJvZYjwc5Nm\CClZXzs M3yxTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
H520  Mni4SpVv[3Srb36gRZN{[Xl? NV[0fIJZOTEEoNM1US=> M2XNSFEzKGh? MUfEUXNQ NWnHXHdo\GWlcnXhd4V{KE2VSEKgcXJPSSCjbnSgdJJwfGWrbjDzeIFjcWyrdImgbY4h\2WoaYTpcoljNXS{ZXH0[YQhVlOFTFOgZ4VtdHN? MnXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
H1703  MmO2SpVv[3Srb36gRZN{[Xl? NXe4NXA6OTEEoNM1US=> NULsZodVOTJiaB?= M1v4NGROW09? NFjKblFl\WO{ZXHz[ZMhVVOKMjDtVm5CKGGwZDDwdo91\WmwIIP0ZYJqdGm2eTDpckBo\W[rdHnubYIufHKnYYTl[EBPW0OOQzDj[Yxtew>? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|OEmwN{c,OjRzM{i5NFM9N2F-
MCF-7  NYjFem5tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITkeFYyOMLizszN NGDZbokzPMLiaB?= M1X4OIlvcGmkaYTzJJRp\SCFUkGwPE1qdmS3Y3XkJINmdGxiZHXheIg> NX:2N4JURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlg5PTNpPkK0NVI5QDV|PD;hQi=>
HPAEpiCs Ml3qSpVv[3Srb36gRZN{[Xl? MljtNE4yNzFxMUCg{txO NEL5O3UyKGh? NWfCfZdXcW6qaXLpeJMhXE6ILd8xMYlv\HWlZXSgZ3BNSTMEoIDyc5RmcW5iYX7kJI1TVkFiZYjwdoV{e2mxbh?= M4\SeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2ME[5NVU5Lz5{NEC2PVE2QDxxYU6=
podocytes M2\rWWZ2dmO2aX;uJGF{e2G7 MUKxNOKh|ryP Mk\XNUBp MWfpcohq[mm2czDUS2bPujFvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFugZY5lKEW{a{GvNuKh MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB|NkKxNkc,OjRyM{[yNVI9N2F-
MCF-7 M3v3W2Z2dmO2aX;uJGF{e2G7 NUHKZWk1OTEEoN88US=> NVnIUGJwOSCq NWPrPW9qTE2VTx?= M17Dc5Jm\HWlZYOgeIhmKFeDLXnu[JVk\WRicHjvd5Bpd3K7bHH0[YQheDN6IF3BVGs> NX[3[XFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkxQTBpPkK0NFE6ODlyPD;hQi=>
MCF-7 NXT4eJZ4TnWwY4Tpc44hSXO|YYm= MVSxNOKh|ryP MmrqNlQhcA>? MlvUSG1UVw>? MnjibY5kemWjc3XzJJRp\SCZQT3pcoR2[2WmIHHwc5B1d3Orcx?= M2noUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG5NFkxLz5{NECxPVA6ODxxYU6=
HAPI MnrrSpVv[3Srb36gRZN{[Xl? M3jXdVExNzJyL{SwxsDPxE1? M4HSNVEhcA>? MmK2bY5pcWKrdIOgWGNFTC2rbnT1Z4VlKHB|OD;KUmshVUGSSzDwbI9{eGixconsZZRqd25? MoLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkmxNlAoRjJ|OU[5NVIxRC:jPh?=
HAPI M4ruZ2Z2dmO2aX;uJGF{e2G7 MXmyNOKh|ryPwrC= MXGxJIg> NGj2bm5FVVOR NWfrb|J{[XS2ZX71ZZRmeyCWQ1TEMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCrTl;TJIFv\CCycn;keYN1cW:w NFj3RY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PVEzOCd-MkO5OlkyOjB:L3G+
HepG2 NHf1ZWpHfW6ldHnvckBCe3OjeR?= MUWzOVAhdk1? NFnkPZAzPCCq NEjkcFRqdmirYnn0d{B1cGViZHXneYVtcW5vaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFu= M{XqZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUOzNVk5Lz5{M{mzN|E6QDxxYU6=
AGS  M3XD[mZ2dmO2aX;uJGF{e2G7 MUmxNOKh|ryP M4rrTFMxKG2rbh?= NXuxfZJRcW6qaXLpeJMh[2G|cHHz[U0{KGGldHn2ZZRqd25iYX7kJIlvcGmkaYTpc44hd2ZiRWLL NX;DdFRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OVA6QTRpPkKzPFUxQTl2PD;hQi=>
HepG2 NGL2XWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TWVFAuPTBizszN MVK0PEBp NF;hVmZqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyN{WwPEc,OjN6MEe1NFg9N2F-
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HL7702 NXrSSHBQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHJSnBsOC13MDFOwG0> NYPxc2V3PDhiaB?= MXrpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyN{WwPEc,OjN6MEe1NFg9N2F-
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MG 63 (6-TG R) NWD2d25MeUiWUzDhd5NigQ>? MnnpdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUWchPjNiKE[tWGchWiliY3XscJM> NFrvOGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh30 NWC3bWFOeUiWUzDhd5NigQ>? MnTKdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog{OCClZXzsdy=> NH[xXFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 NV;RdJFKeUiWUzDhd5NigQ>? NFTXflRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBQUFNvNUCgZ4VtdHN? M1;ONVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NB1643 M1LIfpFJXFNiYYPzZZk> MlKwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ M1fBPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC Mn3FdWhVWyCjc4PhfS=> MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{ M2PQNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NGrpXFFyUFSVIHHzd4F6 MXPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTTk1ISk1{IHPlcIx{ MmC3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
TC32 Ml3QdWhVWyCjc4PhfS=> Mor2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 NI\pPY9yUFSVIHHzd4F6 M3O5Z5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpOThiY3XscJM> NEfzbHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2; 

PubMed: 26844273     


CRC cells were treated with 10 μM SB202190 for 2 h and analyzed for mTOR signaling and on-target inhibition of p38 by immunoblot. 

pJNK1 / pJNK2 / JNK1 / JNK2 ; 

PubMed: 18222647     


A549 cells were treated with DMSO control or with 10 µM of SB202190 for 0.5, 1, 2, 3, 4, or 6 hours, and pJNK and unphosphorylated JNK were detected as described. 

26844273 18222647
Immunofluorescence
p-p38 / MMP9; 

PubMed: 24479681     


Immunocytochemical staining and confocal microscopy confirmed that IL-1β increased activation of p38 (red), and MMP2 and MMP9 (green) expression in MKN-45 GA cells; these effects were blocked by p38 siRNA or the p38 inhibitor SB202190.

24479681
Growth inhibition assay
Cell viability ; 

PubMed: 26844273     


Top panel: CRC cells were treated with various concentrations of SB202190 for 48 h. Lower panel: CRC cells were treated with SB202190 at 10 μM for different times. Cell growth was measured by SRB assay (means ± s.d. in triplet, n = 3 in triplet).

26844273
In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. [5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. [8]

Protocol

Kinase Assay:

[1]

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In vitro kinase assays:

The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-33P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-33P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.
Cell Research:

[2]

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  • Cell lines: Jurkat, and HeLa
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.


    (Only for Reference)
Animal Research:

[5]

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  • Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG
  • Dosages: 12.5 μg
  • Administration: Administered via i.d.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (199.19 mM)
Ethanol 12 mg/mL (36.21 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%H2O
For best results, use promptly after mixing.
8 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.34
Formula

C20H14N3OF

CAS No. 152121-30-7
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID