SB202190 (FHPI)

For research use only.

Catalog No.S1077

80 publications

SB202190 (FHPI) Chemical Structure

Molecular Weight(MW): 331.34

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

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Selleck's SB202190 (FHPI) has been cited by 80 publications

Purity & Quality Control

Choose Selective p38 MAPK Inhibitors

Biological Activity

Description SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.
Targets
p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. [2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. [3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). [4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. [6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 M3fKT2Z2dmO2aX;uJGF{e2G7 NUDxfpl[OjVizszN MU[zNEBucW5? Mk\USG1UV8Li MYDheJRmdnWjdHXzJJRp\SCvUl7BJIFv\CCycn;0[YlvKGW6cILld5Nqd25ib3[gbGJFNTJiaX6gdoV{eG:wc3n2[UB1dyCGQR?= NWTK[5JVOjZ{MkOyOVE>
MDA-MB-231 M3fHc2Z2dmO2aX;uJGF{e2G7 Mo\MNkDPxE1? M{LmeVI1KGh? MVjs[ZN{\W6nczDDR2wzKGmwZIXjeIlwdiCkeTDUUmbPuQ>? MmPGNlYyODB6NEi=
rBMSCs Mnf1SpVv[3Srb36gRZN{[Xl? NV75[2FYOTBizszN MVuyMlUhcA>? MV7k[ZBz\XO|ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\iCHUlugZY5lKHB|OB?= NIG2R4UzPjB3M{K2Oi=>
MG63 M{TVRmZ2dmO2aX;uJGF{e2G7 MXWxNE8zOC9|MDFOwG0> MUOyOEBp MWPzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyC2aHWgcIV3\Wxib3[gdIhwe3Cqb4L5cIF1\WRicEO4JIlv\HWlZXSgZpkhS2SFbEKgbY4h[SClb37j[Y51emG2aX;uJE1l\XCnbnTlcpQhdWGwbnXy NWrXUIVCOjV7OUizNVI>
MG63 MV3BdI9xfG:|aYOgRZN{[Xl? NW[2d3BnOTBxMkCvN|Ah|ryP Mn6yNlQhcA>? NHr0S5F{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnZDD0bIUh[XCxcITvd4l{KHKjdHWgc4YhVUd4MzDpcoR2[2WmIHL5JGNlS2x{ MV[yOVk6QDNzMh?=
HTSMCs NXfBPYZYTnWwY4Tpc44hSXO|YYm= M{PDeFAvOS9zL{GwJO69VQ>? M2XsNFEhcA>? NXXRN25CcW6qaXLpeIVlKEORUl2tNk1qdmS3Y3XkJGhQNTFicILveIVqdiCuZY\lcJMh[W6mIH3SUmEh\XiycnXzd4lwdg>? MUSyOVkzOTR4NB?=
MIA PaCa-2 Ml\iSpVv[3Srb36gRZN{[Xl? MVWyNOKh|ryP MW[yOEBp NVzkPZM6emWmdXPl[EBt[WO2YYTlJIFk[3WvdXzheIlwdiCrbjDjc41jcW6jdHnvckB4cXSqIHLveIghOi2GRzDhcoQhTC2jbHzvd4XDqA>? NVT2fYNFOjV6OEi0PFk>
MIA PaCa-2 Mlf2SpVv[3Srb36gRZN{[Xl? NWnxN24yOjEEoN88US=> M3;LUVI1KGh? NWmxUmY2emW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> M{DUblI2QDh6NEi5
BxPC-3 M4LON2Z2dmO2aX;uJGF{e2G7 MmTUNlDDqM7:TR?= NXXWNY0{OjRiaB?= M{fZUZJme3WudIOgbY4h[SCvb3Tld5QhcW6qaXLpeIlwdiCxZjDITWYuOc7zIIDyc5RmcW5iYXPjeY12dGG2aX;u NYjq[YU3OjV6OEi0PFk>
AsPC-1 NVrqSlBXTnWwY4Tpc44hSXO|YYm= MWKyNOKh|ryP NGLBPGUzPCCq MUDy[ZN2dHS|IHnuJIEhdW:mZYP0JIlvcGmkaYTpc44hd2ZiSFnGMVHPuSCycn;0[YlvKGGlY4XteYxifGmxbh?= NFmycnozPTh6OES4PS=>
MIA PaCa-2 MVfGeY5kfGmxbjDBd5NigQ>? MlPYNlDDqM7:TR?= Mo\qNlQhcA>? Mm[0[Y5p[W6lZYOgZ4xm[X[jZ3Wgc4YhWEGUUDD3bIVvKGOxbXLpcoVlKHerdHig[4x2[2:|ZTDhcoFtd2e| MVSyOVg5QDR6OR?=
MIA PaCa-2 NGXvTllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXmyNOKh|ryP NVnuXVFrOiCq MVLz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> MkXoNlU5QDh2OEm=
BxPC-3 MoX5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVu2[oV[OjEEoN88US=> NFPsR|czKGh? MVnz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> M4PHVVI2QDh6NEi5
AsPC-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PXdFIxyqEQvF2= NXXWZXBiOiCq Mn[yd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm M2fMdVI2QDh6NEi5
HEY NXKzPZVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXOyNOKh|ryP MX6yJIg> M4X0VpNmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? MlPyNlU5QDh2OEm=
OVCAR-3 NF:4TWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\4RYt[OjEEoN88US=> NXTQWHZnOiCq NGe2ZZh{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? MVGyOVg5QDR6OR?=
SK-OV-3 NUXWflNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzRfpMzOMLizszN NUXpcYY5OiCq MYrz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> NIe5W|AzPTh6OES4PS=>
MH7A  MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXiyOEBp M{Dp[ZJmcW6ob4Lj[ZMhfGinIHnubIljcXSxcomg[YZn\WO2czDv[kBZSU5? MmnRNlU5PjJ7Nk[=
MH7A  MXnBdI9xfG:|aYOgRZN{[Xl? NYLscnJsOjVizszN MkntNlQhcA>? NE[0Nm9z\X[ncoPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBqdmS3Y3XkJIJ6KFiDTjDhcoQh[2G3c3XkJIFxd3C2b4Ppd{Bw\iClZXzsd{B3cWFiYXP0bZZifGmxbjDv[kBLVkt? MkfoNlU5PjJ7Nk[=
SCC25 NUHnb2x6TnWwY4Tpc44hSXO|YYm= MUiyNEDPxE1? NWn1Zmp6OjRiaB?= M{XTcolv[3KnYYPld{BifXSxcHjh[5khdGW4ZXy= MYGyOVg{PDRyMB?=
HaCaT  NFO3N2pHfW6ldHnvckBCe3OjeR?= Mk\hOUDDvU1? NF7NNFczPCCq NXP1U25CcW6qaXLpeJMhUU[QLd8zMYlv\HWlZXSgR2NNOjJicILv[JVkfGmxbjDs[ZZmdHN? NXrXZmMxOjV6M{SzOVM>
HaCaT  M2\JdWZ2dmO2aX;uJGF{e2G7 MV[1JOK2VQ>? NHLVOo0zPCCq NWC4VHg3cW6qaXLpeJMhUU[QLd8xMYlv\HWlZXSgR2NNOjJicILv[JVkfGmxbjDs[ZZmdHN? M1LXSFI2QDN2M{Wz
HPAEpiCs  NHS2UFFHfW6ldHnvckBCe3OjeR?= MWSxM|MwOTBizszN M2LBXVEhcA>? MY\y[YR2[2W|IGOxVE1qdmS3Y3XkJGlESU1vMTDwdo91\WmwIHHu[EBuWk6DIHX4dJJme3Orb36gZY5lKHC{b33veIVzKGGldHn2bZR6 MlPMNlU4OzR7MEC=
HPAEpiCs NWLFW5AyTnWwY4Tpc44hSXO|YYm= NGnNW2kyNzNxMUCg{txO M1Pmd|EhcA>? M{HtRYlvcGmkaYTzJHMyWC2|dHnteYxifGWmIFHreEBxcG:|cHjvdplt[XSrb36gJOKh MWSyOVc{PDlyMB?=
HPAEpiCs NH;qfZhHfW6ldHnvckBCe3OjeR?= MoS1NU8{NzFyIN88US=> MWCxJIg> M3eyS4lvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u MlHXNlU4OzR7MEC=
K562 MXHGeY5kfGmxbjDBd5NigQ>? NIPTO40yOMLizszN MX:xxsBp NVfU[oI6TE2VTx?= M2jud4lvcGmkaYTzJJF2cW6jY4LpcoUucW6mdXPl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36= NX[3SnNMOjV4OESwOFM>
PANC-1 NFHsW45HfW6ldHnvckBCe3OjeR?= NXzSfW5TOTBizszN M2fCOVEhcA>? MVjlcohidmOnczD0bIUh[XW2b4DoZYdq[yCnZn\lZ5TDqA>? MnS2NlU3OzJ{MkK=
BxPC-3 MoXySpVv[3Srb36gRZN{[Xl? NWP1WI01OTBizszN M3PvOVEhcA>? NYS3V3Bm\W6qYX7j[ZMhfGinIHH1eI9xcGGpaXOg[YZn\WO2wrC= NELTO|YzPTZ|MkKyNi=>
K562  NWfiPVZ3TnWwY4Tpc44hSXO|YYm= M2DTflAvOjVvMTFOwG0> MlvmNlQhcA>? MmLRd5VxeHKnc4Pld{Bz\XO4ZYLheJJwdC2rbnT1Z4VlKEh{QWigdIhwe3Cqb4L5cIF1cW:w NEfhZWgzPTZzOUO5Ni=>
THP-1 MXvGeY5kfGmxbjDBd5NigQ>? NVfXWmZsPSEEtV2= NHHadnozKGh? NHfsPYJ{cWewaX\pZ4FvfGy7IHH0eIVvfWG2ZYOgd4VkemW2aX;uJI9nKEmOLUJOtUBqdmS3Y3XkJIJ6KDJ5T1jDbI9tKHCudYOgSnNNNTIEoB?= MmL5NlU2QTh4NkG=
WB MmHFSpVv[3Srb36gRZN{[Xl? MmHTNlDjiIoQvF5CpC=> NWSyZZZyOzEkgJntbY4> M37GXoRm[3KnYYPld{B1cGViTGDTMUBweiCOVFGtbY5lfWOnZDDJUE03KGGwZDDUUmYu|rIEoIDyc4R2[3Srb36= NIfXUFEzPTV|ME[4Ni=>
RAW 264.7 MUfGeY5kfGmxbjDBd5NigQ>? M1vFcVExyqEQvF2= NWTPSJl1OzEkgJntbY4> NFfYVY1qdmS3Y3XzJINp[XKjY4Tldol{fGmlII\hZ5VwdGG2aX;uJI9nKE:Fc9Mg M3rZS|I2PDZzM{m5
RAW 264.7 NYHqRW5yTnWwY4Tpc44hSXO|YYm= M1zwVlExyqEQvF2= MkHCN|DjiImvaX6= M4LFXYF1fGWwdXH0[ZMhfGinIHXm[oVkfHNib3[gU3BIKG:wIH;zeIVw[2yjc4SgdoV1emGldHnvci=> NXPrVVNCOjV2NkGzPVk>
HaCaT  MVjGeY5kfGmxbjDBd5NigQ>? NWPDV3k2PDEEoN88US=> NX;jNXBkOy1{NDDo NHHVepZFVVOR MV\y[YR2[2W|IITo[UBi[2O3bYXsZZRqd25ib3[gXm8uOQ>? NIixToEzPTR|NUS4OS=>
H9c2 NFnTem5HfW6ldHnvckBCe3OjeR?= MnjyOVDDqM7:TdMg NF3z[oEyOiCq NYSxbIY{emWmdXPld{BNTEhicnXs[YF{\SCjbnSgUW1RKGyxc4O= M365e|I2OjR3OEG4
HSCs NV31[XE5SXCxcITvd4l{KEG|c3H5 NXTEUYpjOjVizszN MUeyOOKhcA>? MVHzbYdvcW[rY3HueIx6KGG2dHXueYF1\XNiVFetbY5lfWOnZDDhZ5RqfmG2ZXSgTHNEeyCjcH;weI9{cXN? NVv2U4pxOjR7NkG5OVA>
THP-1 NHf0[GhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnqU25vPzJiaB?= MnHpSG1UVw>? MVrJR|UxRTRwN988US=> MmPyNlQ5OTVyOEe=
MDDCs NGnNWJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX23NkBp MmrmSG1UVw>? MlzMTWM2ODJwN988US=> MkWwNlQ5OTVyOEe=
MDDCs NX:4c4xOTnWwY4Tpc44hSXO|YYm= NGXHRpIxNTF3IN88US=> NXj6VFdQPDhiaB?= NGexWXpFVVOR MVrzeZBxemW|c3XzJGlHVi4QsTDhcoQhUVBvMUCgdJJw\HWldHnvcuKh MWCyOFgyPTB6Nx?=
MDDCs MlXhSpVv[3Srb36gRZN{[Xl? MofvNE0yPSEQvF2= MYq0PEBp NH\zU21FVVOR NUHTdZJ5cW6qaXLpeJMhVUmSLUHhMEBOUVBvMXKgZY5lKFKDTmTFV{Bxem:mdXP0bY9v M2fpXVI1QDF3MEi3
MDDCs MVHGeY5kfGmxbjDBd5NigQ>? M3XGWVExKM7:TR?= MnPhN{42KGh? M1[xe2ROW09? MULicI9kc3NiRVLPWkBIWCxiYoX0JI5wfCCYU2[gS{Bu\WSrYYTl[EBmdnS{eTDpcpRwKGi3bXHuJG1FTEO| NITTNZgzPDhzNUC4Oy=>
macrophages NHnhfFZHfW6ldHnvckBCe3OjeR?= Mm\UNgKBkc7:TR?= M4\BbFQvPeLCiXi= MnS5Z49ueGyndHXsfUBqdmirYnn0d{BOXC2LSVmtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKE6ILd86Ri=> MVmyOFgxQDZ|Mx?=
PDL  NF7QbW9HfW6ldHnvckBCe3OjeR?= NYLZW2ltOjEEoN88UeKh NEDuc5g{OCCvaX6= MnzBSG1UVw>? MWjzbYdvcW[rY3HueIx6KGmwaHnibZR{KHSqZTD0[Y5{cWynIH\vdoNmNW2nZHnheIVlKEKPUD2yJIV5eHKnc4Ppc44> NVjG[2hoOjR3NkGwPFE>
AGS NYn4NW1DTnWwY4Tpc44hSXO|YYm= M3f1Z|Uh|ryP NXjhNVVUOzBibXnu NFPMTox{fXCycnXzd4V{yqBzLXnu[JVk\WRiY3HzdIF{\S16IHHu[EBk[XOyYYPlMVMh[WO2aY\heIlwdg>? NGrKT2kzPDV2N{i3PC=>
H520 MXnGeY5kfGmxbjDBd5NigQ>? M1nlV|ExyqEEtV2= NYTvTnFyOSCq NWXtbZdGTE2VTx?= MUjk[YNz\WG|ZYOgeIhmKHCnbXX0doV5\WRvaX7keYNm\CCPU1iyJI1TVkFiYX7kJJBzd3SnaX6gcIV3\Wy| M37hOVI1PTNyNEe1
H1703 Mn;5SpVv[3Srb36gRZN{[Xl? NFjnZ2gyOMLiwsXN MkPINUBp MlG3SG1UVw>? Mmq3[IVkemWjc3XzJJRp\SCyZX3leJJmgGWmLXnu[JVk\WRiTWPINkBuWk6DIHHu[EBxem:2ZXnuJIxmfmWucx?= NIPZWHozPDV|MES3OS=>
H520 M3;sSmZ2dmO2aX;uJGF{e2G7 Moq5NVDDqML3TR?= MnqzNVIhcA>? MV;EUXNQ MVHpcohq[mm2czDw[Y1mfHKneHXkMYVtcWOrdHXkJG1UUDJicILveIVqdiC|dHHibYxqfHl? NIrOTZkzPDV|MES3OS=>
H1703 NV7YcGJ5TnWwY4Tpc44hSXO|YYm= NEjLUY0yOMLiwsXN MXOxNkBp NHHiV5hFVVOR NIXxfGNqdmirYnn0d{Bx\W2ndILlfIVlNWWuaXPpeIVlKE2VSEKgdJJwfGWrbjDzeIFjcWyrdIm= MlLwNlQ2OzB2N{W=
H520 NF6yeVhHfW6ldHnvckBCe3OjeR?= Ml\MNVDDqML3TR?= NWf3VW11PiCq MV;EUXNQ MYDzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyC2aHWgcIV3\Wy|IH;mJJVjcXG3aYTpck1kd26sdXfheIVlKE2VSEKgbY4heGWvZYTy[Zhm\C22cnXheIVlyqClZXzsJIxqdmV? NInTUYIzPDV|MES3OS=>
H1703 NYnSU4ExTnWwY4Tpc44hSXO|YYm= M3iydVExyqEEtV2= NF7pdIg3KGh? NYDwSmdZTE2VTx?= M1fVVpNq\26rZnnjZY51dHliaX7jdoVie2W|IITo[UBt\X[nbIOgc4YhfWKrcYXpeIlvNWOxbnr1[4F1\WRiTWPINkBqdiCyZX3leJJmgGWmLYTy[YF1\WUEoHPlcIwhdGmwZR?= Ml32NlQ2OzB2N{W=
MC3T3-E1 MUDGeY5kfGmxbjDBd5NigQ>? NES3UZoxNjNxMz:zNEDPxE1? MkG1NUBp Mm\5ZZR1\W63YYTld{BVVkZvzsGtbY5lfWOnZDDNUXAuQSCneIDy[ZN{cW:wIHnuJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgcYFvdmW{wrC= NWCweHhmOjR3MEK2PVY>
MC3T3-E1 NEXYN41HfW6ldHnvckBCe3OjeR?= NF\iN4w{OCEQvF2= MoO1NUBp NVH1Wm5F[XS2ZX71ZZRmeyCWTl[t{tEue3SrbYXsZZRm\CCyM{igUWFRUyCyaH;zdIhwenmuYYTpc44> M17KNlI1PTB{Nkm2
HUVECs  MX3GeY5kfGmxbjDBd5NigQ>? M2\yPFExyqEEtV2= Mo\PNUBp NEjScnZqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDDXGNNOSCycn;keYN1cW:w M325O|I1PDh5OU[0
AGS  NYXxbItUTnWwY4Tpc44hSXO|YYm= NHPVXIMyOMLiwsXN Mn3NN|DDqG2rbh?= MV3pcohq[mm2czDJUE0y|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>? Mk\5NlQ1Pzl4OEG=
MKN-45 MkjlSpVv[3Srb36gRZN{[Xl? NXPXfop5OTEEoNM1US=> MmOyN|DDqG2rbh?= NEnKO5ZqdmirYnn0d{BKVC1zzsKtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB|OB?= M3HndFI1PDd7Nkix
AGS  NIXWOYVHfW6ldHnvckBCe3OjeR?= M3PVcFExyqEEtV2= NUjPS2NSOzEEoH3pci=> NILteI1ifHSnboXheIV{KEmOLUJOtk1qdmS3Y3XkJGdCKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdg>? NFriNpMzPDR5OU[4NS=>
MKN-45 MWjGeY5kfGmxbjDBd5NigQ>? NH7Jb|EyOMLiwsXN NF[5UFA{OMLibXnu MXXheJRmdnWjdHXzJGlNNTIQsj3pcoR2[2WmIFfBJINmdGxibXnndoF1cW:wIHHu[EBqdn[jc3nvci=> M3;pXVI1PDd7Nkix
AGS  NH70PVFHfW6ldHnvckBCe3OjeR?= MkPqNVDDqML3TR?= NEjLWFk{OMLibXnu M1HudpNq\26rZnnjZY51dHliZHXjdoVie2W|IFnsMVHPui2rbnT1Z4VlKE2PUEKgZY5lKE2PUEmgcXJPSSCneIDy[ZN{cW:w MknFNlQ1Pzl4OEG=
MKN-45 MoLGSpVv[3Srb36gRZN{[Xl? M3H3OVExyqEEtV2= NY\oe3hrOzEEoH3pci=> NF3WOGx{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDJcE0y|rJvaX7keYNm\CCPTWCyJIFv\CCPTWC5JI1TVkFiZYjwdoV{e2mxbh?= MVOyOFQ4QTZ6MR?=
DCs MoDoSpVv[3Srb36gRZN{[Xl? NV62RWFLOjEEoN88US=> M1;q[|HDqGh? MVHk[YNz\WG|ZYOgTWwuOTJicILv[JVkfGmxbh?= Ml3KNlQ1OzR4M{[=
HUVEC  NHX3d4FHfW6ldHnvckBCe3OjeR?= M4HH[VIxKM7:bdMg NGPtXJc2KGh? NY\SWZdXTE2VTx?= MmnXdoVlfWOnczDjfZRwc2mwZTDlfJBz\XO|aX;uJIxmfmWuczDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nch?= NH3yUIQzPDF6OUC2Ni=>
A 549 NVrrfYtoTnWwY4Tpc44hSXO|YYm= NH;yVYk2OCEQvF2= NHrFe3EyKGh? NHT4bVhl\WO{ZXHz[ZMhfGinIHzleoVtKG:oIFnMMVjDqA>? MUmyOFE4QTZ6OB?=
H520  Mlr3SpVv[3Srb36gRZN{[Xl? NUW4Z2piPS9zMDFOwG0> NInNPZIyKGh? NXnwTHY1TE2VTx?= NFjMTVNl\WO{ZXHz[ZMhVVOKMjDwdo91\WmwIHHzJJdmdGxiYYOgcXJPSSCuZY\lcJMhcW5iZ3XmbZRqdmmkLXX4dI9{\WRiY3XscC=> M4nsW|I1OTN6OUCz
H1703  M1PFS2Z2dmO2aX;uJGF{e2G7 NV;M[255PS9zMDFOwG0> MnrZNUBp NW[1UZFVTE2VTx?= MljD[IVkemWjc3XzJG1UUDJicILveIVqdiCjczD3[YxtKGG|IH3SUmEhdGW4ZXzzJIlvKGenZnn0bY5q[i2neIDvd4VlKGOnbHy= M1[2c|I1OTN6OUCz
H520  NVrTNIpnTnWwY4Tpc44hSXO|YYm= NYPndZlEOTEEoNM1US=> NWjQb4t1OTJiaB?= NFzFRodFVVOR MWLk[YNz\WG|ZYOgUXNJOiCvUl7BJIFv\CCycn;0[YlvKHO2YXLpcIl1gSCrbjDn[YZqfGmwaXKteJJm[XSnZDDOV2NNSyClZXzsdy=> MViyOFE{QDlyMx?=
H1703  MnL0SpVv[3Srb36gRZN{[Xl? NHvrUWgyOMLiwsXN M{XkbFEzKGh? NH;UdXJFVVOR M{nNXYRm[3KnYYPld{BOW0h{IH3SUmEh[W6mIIDyc5RmcW5ic4ThZoltcXS7IHnuJIdm\mm2aX7pZk11emWjdHXkJG5US0yFIHPlcIx{ MoLnNlQyOzh7MEO=
MCF-7  M4DDT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nSeVExyqEQvF2= NGfwV4szPMLiaB?= M{jFXolvcGmkaYTzJJRp\SCFUkGwPE1qdmS3Y3XkJINmdGxiZHXheIg> NIDWVmozPDF{OEi1Ny=>
HPAEpiCs NFHu[I1HfW6ldHnvckBCe3OjeR?= NFv0U2YxNjFxMT:xNEDPxE1? NYHBTmZTOSCq NGToTWtqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDjVGxCOsLicILveIVqdiCjbnSgcXJPSSCneIDy[ZN{cW:w NFvW[HEzPDB4OUG1PC=>
podocytes NXPzcHZ6TnWwY4Tpc44hSXO|YYm= NH;KXI8yOMLizszN M3HK[VEhcA>? NUDXOZFKcW6qaXLpeJMhXEeIzsKxMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCyM{jNRXBMKGGwZDDFdosyNzMEoB?= M3fReVI1ODN4MkGy
MCF-7 NV[wO3lTTnWwY4Tpc44hSXO|YYm= MWSxNOKh|ryP M3jwVlEhcA>? Mn7PSG1UVw>? M{P1ZZJm\HWlZYOgeIhmKFeDLXnu[JVk\WRicHjvd5Bpd3K7bHH0[YQheDN6IF3BVGs> MUmyOFAyQTB7MB?=
MCF-7 NWLaU2VuTnWwY4Tpc44hSXO|YYm= MV:xNOKh|ryP NVfBOoszOjRiaB?= MmLwSG1UVw>? NUPx[41pcW6lcnXhd4V{KHSqZTDXRU1qdmS3Y3XkJIFxd3C2b4Ppdy=> M{XzN|I1ODF7MEmw
HAPI NF\tbWNHfW6ldHnvckBCe3OjeR?= MnXUNVAwOjBxNEFCpO69VQ>? NVTyfmJFOSCq MnWybY5pcWKrdIOgWGNFTC2rbnT1Z4VlKHB|OD;KUmshVUGSSzDwbI9{eGixconsZZRqd25? MkLtNlM6PjlzMkC=
HAPI M4jMRWZ2dmO2aX;uJGF{e2G7 MX6yNOKh|ryPwrC= NWjEb3BpOSCq MmXkSG1UVw>? M1jLfIF1fGWwdXH0[ZMhXEOGRD3pcoR2[2WmIHHjeIl3[XSrb36gc4YhcU6RUzDhcoQheHKxZIXjeIlwdg>? NHTXVFIzOzl4OUGyNC=>
HepG2 M2HwUmZ2dmO2aX;uJGF{e2G7 NHHjbG8{PTBibl2= MXiyOEBp M{nQSYlvcGmkaYTzJJRp\SCmZXf1[Yxqdi2rbnT1Z4VlKGGldHn2ZZRqd25ib3[gdFM5VUGSSx?= NIPDU40zOzl|M{G5PC=>
AGS  NWjCW5VuTnWwY4Tpc44hSXO|YYm= M3;wPFExyqEQvF2= MUSzNEBucW5? MknVbY5pcWKrdIOgZ4F{eGG|ZT2zJIFkfGm4YYTpc44h[W6mIHnubIljcXSrb36gc4YhTVKN NVzze41kOjN6NUC5PVQ>
HepG2 MlHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{Ps[FAuPTBizszN NEPEcm81QCCq NFTjO|hqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> NHnXUG4zOzhyN{WwPC=>
BEL7404 MmD6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXOwMVUxKM7:TR?= Ml70OFghcA>? MYrpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= NIrzRo0zOzhyN{WwPC=>
HL7702 M1Gzbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzFW45ZOC13MDFOwG0> NH7aRoE1QCCq M2D5c4lvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIlvKGFiZH;z[UBl\XCnbnTlcpQhdWGwbnXy MUCyN|gxPzVyOB?=
HepG2 NV7NWHBkTnWwY4Tpc44hSXO|YYm= MXuwMVUxKM7:TR?= MWqyOEBp NGH6d2ZqdmirYnn0d{B1cGVicHjvd5Bpd3K7bHH0bY9vKG:oIICzPEBld3ewc4Ty[YFuKHC{b4TlbY5{KE2DUFvBVGszNCCDVF[yMEBOW0tzIHHu[EBJW1B{NzDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MorFNlM5ODd3MEi=
BEAS-2B NFjlU2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUOxNEDPxE1? NWO0Z5pQOzBibXnu M4XaZpJmfmW{c3XzJJRp\SCmZXPy[YF{\SCxZjDj[YxtKH[rYXLpcIl1gSCrbnT1Z4VlKGK7IFjDTS=> NUPEPZRxOjN5OESwN|Q>
BEAS-2B NXn5Ro5MS3m2b4TvfIl1gSCDc4PhfS=> M2f1clExKM7:TR?= MkP2N|AhdWmw NFHlNHNqdmirYnn0[YQhfGinIHnuZ5Jm[XOnIHnuJGxFUCCjbnSgTWwuQCCneIDy[ZN{cW:w M{LwN|I{Pzh2MEO0
BEAS-2B MkDISpVv[3Srb36gRZN{[Xl? NX\BenZtOTBizszN MXqzNEBucW5? Momy[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZiY3HzdIF{\S1|LDDCZYQh[W6mIH\hdy=> NWXod5RlOjN5OESwN|Q>
H9c2  MVzGeY5kfGmxbjDBd5NigQ>? M2DEW|AvODFxMD6xM|Eh|ryP MkjUNUBp MmqyZZR1\W63YYTld{BVVkZvzsGtbY5lfWOnZDDNUXAuQSCneIDy[ZN{cW:wLDDtVm5CKGyndnXsd{wh[W6mIIDyc41wfGW{IHHjeIl3cXS7 M1HQZVI{Pzd2MkWy
H9c2  NIfXSJNHfW6ldHnvckBCe3OjeR?= M2PZbVEh|ryP NGriem0yKGh? NIT1eWtz\WS3Y3XzJHRPTi4QsTDkbZJm[3SueTDzeIlufWyjdHXkJJA{QCCPQWDLJJBpd3OyaH;yfYxifGmxbh?= NHfEZ5czOzd5NEK1Ni=>
U937 NYW2bWZ3TnWwY4Tpc44hSXO|YYm= NIDr[FkyOMLizszNxsA> M3\nblEhcA>? MX;hZpJw\2G2ZYOgeIhmKGOjZn\lbY5mKGWoZnXjeEBwdiCPS2CtNUBidmRiUGCyRYPPuSCvUl7BJJRz[W6|Y4LpdJRqd26jbDDs[ZZmdHN? MnjzNlM4ODd|OEe=
U937 M33PemZ2dmO2aX;uJGF{e2G7 MkPrNVDDqM7:TdMg MlHXNUBp NHi5Zoli[nKxZ3H0[ZMh[2GoZnXpcoUucW6mdXPl[EBOU1BvMTDkc5dvNXKnZ4XsZZRqd25iYX7kJHBROkGlzsGgeZAuemWpdXzheIlwdg>? NFTwSZIzOzdyN{O4Oy=>
U937 MWTGeY5kfGmxbjDBd5NigQ>? M1jQblExyqEQvF5CpC=> NXzxeod2OSCq M1vKW5N2eHC{ZYPz[ZMh[y2MdX6gZY5lKEOURVKgdIhwe3Cqb4L5cIF1cW:wIHnuJINi\m[naX7lMZRz\WG2ZXSgZ4VtdHQEoB?= MnT5NlM4ODd|OEe=
A549 MWPGeY5kfGmxbjDBd5NigQ>? M{LUUlAvOy9|L{OwJO69VQ>? NXnkOVNDOSCq MVLzbYdvcW[rY3HueIx6KGG2dHXueYF1\XNiQWTQ{tNUNW2nZHnheIVlKEORWD2yJJBzd3SnaX6gZY5lKG2UTlGg[ZhxemW|c3nvckBidmRicILvcY91\XJiYXP0bZZqfHoEoB?= NX\oU2E6OjN4OEC2O|Q>
A549 NWHWRZlDTnWwY4Tpc44hSXO|YYm= MnHJNVDDqM7:TdMg NEfXbYcxNTNyIH3pci=> NV32PFlpcW6qaXLpeJMhSVSSzsPTJIlv\HWlZXSgdFQzN3B2NDDNRXBMKGGwZDDwN|ghVUGSSzDwbI9{eGixconsZZRqd25? M2raSlI{PjhyNke0
A549 NXLXWmF5TnWwY4Tpc44hSXO|YYm= NFPKUIcyOMLizszNxsA> NVS0PWhsOSCq Mmi5bY5pcWKrdIOgRXRR|rOVIHnu[JVk\WRiTl[t{tpDKHB4NTDzeYJ2dmm2IIDoc5NxcG:{eXzheIlwdiCjbnSgUmYu|rqEIIDyc41wfGW{IHHjeIl3cXS7 NGXtdZczOzZ6ME[3OC=>
A549 NXvCcYhVTnWwY4Tpc44hSXO|YYm= NGfNcFMyOMLizszNxsA> NFLoXmkyKGh? NYjNUXBPemWmdXPld{BCXFEQs2Otd5RqdXWuYYTl[EBkWEyDMjDwbI9{eGixconsZZRqd28EoB?= NXnadlJoOjN4OEC2O|Q>
A549 NHjZSodHfW6ldHnvckBCe3OjeR?= MkCzNVDDqM7:TdMg MXuxJIg> M372SZJm\HWlZYOgRXRR|rOVLXXubIFv[2WmIHXufplu[XSrYzDhZ5Rqfmm2eTDv[kBkWEyDMtMg MmjaNlM3QDB4N{S=
PC12 MX;GeY5kfGmxbjDBd5NigQ>? MV[xNE8zOC92MDFOwG0> NEDnTWsyyqCq MX\pcohq[mm2czDKUmsh[W6mIICzPC=> M37EZVI{PTh2M{W3
HK-2 MmjCRZBweHSxc3nzJGF{e2G7 MWiyNOKh|ryP MnfXNlQhcA>? MVXpcohq[mm2czDFVmsh[W6mIICzPG1CWEt? MX2yN|U1OzF3MR?=
H9c2 MXjGeY5kfGmxbjDBd5NigQ>? M1\HN|HDqM7:TR?= NFT4S3gyKGh? NV3HZnVxemWmdXPld{BVVkZvzsGtbY5lfWOnZDDNUXAuQSCvUl7BJIxmfmWuczDhcoQheHKxbX;0[ZIh[WO2aY\peJk> M1vSWlI{OzV|Nkm5
H9c2 NITsVZhHfW6ldHnvckBCe3OjeR?= M37nfFHDqM7:TR?= NIeyPZgyKGh? M2f4[5Jm\HWlZYOgWG5HNc7zLXXubIFv[2WmIFHQMVEheHKxbX;0[ZIh[WO2aY\peJk> NHryT5MzOzN3M{[5PS=>
H1650  NYDSfJhlTnWwY4Tpc44hSXO|YYm= MkHFNVDDqM7:TdMg M4X6[VEhcA>? M4\6d2ROW09? NEfhW4pl\WO{ZXHz[ZMh[m:2aDDwdo91\WmwIHHu[EBuWk6DIHzleoVteyCxZjDFVmNEOSCrbjDwZYNtcXSjeHXsMYV5eG:|ZXVCpINmdGy| M4D2WFI{OjJ6Nkm2
H1703 MmHMSpVv[3Srb36gRZN{[Xl? NH[1N|IyOMLizszNxsA> M2DpVlEhcA>? MYDEUXNQ MkHS[IVkemWjc3XzJIJwfGhicILveIVqdiCjbnSgcXJPSSCuZY\lcJMhd2ZiRWLDR|EhcW5icHHjcIl1[XinbD3lfJBwe2WmwrDj[Yxtew>? M3r4d|I{OjJ6Nkm2
H1650  MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPIelM1OTEEoN88UeKh NFL5[|AyKGh? MlzXSG1UVw>? M{fWXYVvcGGwY3XzJJBi[2yrdHH4[YwucW6mdXPl[EBkgXSxdH;4bYNqfHl? NX3Ib2xWOjN{Mki2PVY>
H1703 NXq3TItvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELJZWgyOMLizszNxsA> NYr1eYFKOSCq MYDEUXNQ Mnm0[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>? M2HFTlI{OjJ6Nkm2

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2; 

PubMed: 26844273     


CRC cells were treated with 10 μM SB202190 for 2 h and analyzed for mTOR signaling and on-target inhibition of p38 by immunoblot. 

pJNK1 / pJNK2 / JNK1 / JNK2 ; 

PubMed: 18222647     


A549 cells were treated with DMSO control or with 10 µM of SB202190 for 0.5, 1, 2, 3, 4, or 6 hours, and pJNK and unphosphorylated JNK were detected as described. 

26844273 18222647
Immunofluorescence
p-p38 / MMP9; 

PubMed: 24479681     


Immunocytochemical staining and confocal microscopy confirmed that IL-1β increased activation of p38 (red), and MMP2 and MMP9 (green) expression in MKN-45 GA cells; these effects were blocked by p38 siRNA or the p38 inhibitor SB202190.

24479681
Growth inhibition assay
Cell viability ; 

PubMed: 26844273     


Top panel: CRC cells were treated with various concentrations of SB202190 for 48 h. Lower panel: CRC cells were treated with SB202190 at 10 μM for different times. Cell growth was measured by SRB assay (means ± s.d. in triplet, n = 3 in triplet).

26844273
In vivo Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. [5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. [8]

Protocol

Kinase Assay:

[1]

- Collapse

In vitro kinase assays:

The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-33P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-33P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.
Cell Research:

[2]

- Collapse
  • Cell lines: Jurkat, and HeLa
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.


    (Only for Reference)
Animal Research:

[5]

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  • Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG
  • Dosages: 12.5 μg
  • Administration: Administered via i.d.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (199.19 mM)
Water Insoluble
Ethanol '12 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%H2O
For best results, use promptly after mixing.
8 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.34
Formula

C20H14N3OF

CAS No. 152121-30-7
Storage powder
in solvent
Synonyms N/A
Smiles OC1=CC=C(C=C1)C2=NC(=C([NH]2)C3=CC=NC=C3)C4=CC=C(F)C=C4

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID