SB202190 (FHPI)

Catalog No.S1077

For research use only.

SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.

SB202190 (FHPI) Chemical Structure

CAS No. 152121-30-7

Selleck's SB202190 (FHPI) has been cited by 141 publications

Purity & Quality Control

Choose Selective p38 MAPK Inhibitors

Other p38 MAPK Products

Biological Activity

Description SB202190 (FHPI) is a potent p38 MAPK inhibitor targeting p38α/β with IC50 of 50 nM/100 nM in cell-free assays, sometimes used instead of SB 203580 to investigate potential roles for SAPK2a/p38 in vivo. SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1. SB202190 significantly suppresses Erastin‐dependent ferroptosis.
Targets
Ferroptosis [11] p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
In vitro

SB 202190 significantly inhibits both basal and anti-Fas antibody-induced MAPKAPK 2 activity in a dose-dependent manner. SB202190 by itself is sufficient to induce cell death in Jurkat and HeLa cells through activation of CPP32-like caspases, which can be blocked by expression of bcl-2. SB202190-induced apoptosis is attenuated by p38β but augmented by p38α. [2] SB 202190 strongly inhibits UVB induced COX-2 protein expression in HaCaT cells, and markedly inhibits UVB induced cox-2 mRNA. [3] SB 202190 treatment inhibits the expression of albumin-induced proinflammatory (monocyte chemoattractant protein-1) and transforming growth factor (TGF)-beta1-induced profibrotic (procollagen-Ialpha1) genes over 50% in renal tubular cells (normal rat kidney-52E). [4] SB 202190 treatment induces phosphorylation of JNK in a dose- and time- dependent manner in A549 cells, induces phosphorylation of ATF-2 transcription factor, and increases AP-1 DNA binding. [6] SB 202190 treatment enhances the growth of THP-1 and MV4-11 cells. SB 202190 increases the phosphorylation of c-Raf and ERK, suggesting that Ras-Raf-MEK-mitogen-activated protein kinase (MAPK) pathway activation is involved in the leukemia cell growth induced by SB 202190. [7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 NFrlNJVHfW6ldHnvckBCe3OjeR?= NF;JOGgzPSEQvF2= MYizNEBucW5? NVn1NHhnTE2VT9Mg NF\DcmRifHSnboXheIV{KHSqZTDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36gc4YhcEKGLUKgbY4hemW|cH;ud4l3\SC2bzDERS=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ{M{K1NUc,OjZ{MkOyOVE9N2F-
MDA-MB-231 MXLGeY5kfGmxbjDBd5NigQ>? M4nUeVIh|ryP NGHNZlYzPCCq MoTCcIV{e2WwZYOgR2NNOiCrbnT1Z5Rqd25iYomgWG5H|rF? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFyMEi0PEc,OjZzMEC4OFg9N2F-
rBMSCs MX3GeY5kfGmxbjDBd5NigQ>? MmPjNVAh|ryP MWKyMlUhcA>? Mn3m[IVxemW|c3XzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiRWLLJIFv\CCyM{i= M3myOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEWzNlY3Lz5{NkC1N|I3PjxxYU6=
MG63 M3fUcGZ2dmO2aX;uJGF{e2G7 NHTlVHIyOC9{MD:zNEDPxE1? M{f5TFI1KGh? M2m0RZNq\26rZnnjZY51dHliZHXjdoVie2W|IITo[UBt\X[nbDDv[kBxcG:|cHjvdplt[XSnZDDwN|ghcW6mdXPl[EBjgSCFZFPsNkBqdiCjIHPvcoNmdnS{YYTpc44hNWSncHXu[IVvfCCvYX7u[ZI> M3\nVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUm4N|EzLz5{NUm5PFMyOjxxYU6=
MG63 NVvM[ZA1SXCxcITvd4l{KEG|c3H5 Ml;oNVAwOjBxM{Cg{txO Ml7ENlQhcA>? NWnS[VBRe2mpbnnmbYNidnSueTDk[YNz\WG|ZXSgeIhmKGGyb4D0c5NqeyC{YYTlJI9nKE2JNkOgbY5lfWOnZDDifUBE\EOuMh?= NWjRSpY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PVg{OTJpPkK1PVk5OzF{PD;hQi=>
HTSMCs M2TjZmZ2dmO2aX;uJGF{e2G7 NY\XXlRHOC5zL{GvNVAh|ryP M3;YZVEhcA>? NGH6NmlqdmirYnn0[YQhS0:UTT2yMYlv\HWlZXSgTG8uOSCycn;0[YlvKGyndnXsd{BidmRibWLORUBmgHC{ZYPzbY9v NG\GS|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUmyNVQ3PCd-MkW5NlE1PjR:L3G+
MIA PaCa-2 NYDjOGdNTnWwY4Tpc44hSXO|YYm= MYeyNOKh|ryP NHHmVHAzPCCq MoHQdoVlfWOnZDDsZYN1[XSnIHHjZ5VufWyjdHnvckBqdiClb33ibY5ifGmxbjD3bZRpKGKxdHigNk1FTyCjbnSgSE1idGyxc3ZCpC=> MmnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
MIA PaCa-2 M{Lhc2Z2dmO2aX;uJGF{e2G7 Mn:yNlDDqM7:TR?= M13STFI1KGh? NI\aUHFz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=> Mme4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
BxPC-3 MnjsSpVv[3Srb36gRZN{[Xl? M3zJ[VIxyqEQvF2= M3e4VlI1KGh? NYjqW4FUemW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> NEfTSog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
AsPC-1 NE\LXXZHfW6ldHnvckBCe3OjeR?= NXjY[WFGOjEEoN88US=> M3i1[VI1KGh? MoTLdoV{fWy2czDpckBiKG2xZHXzeEBqdmirYnn0bY9vKG:oIFjJSk0y|rFicILveIVqdiCjY3P1cZVt[XSrb36= NGjqR2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2 MnrqSpVv[3Srb36gRZN{[Xl? NYCyPY1LOjEEoN88US=> NHLDS3MzPCCq NFvJSVRmdmijbnPld{BkdGWjdnHn[UBw\iCSQWLQJJdp\W5iY3;tZolv\WRid3n0bEBodHWlb4PlJIFv[WyxZ4O= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
MIA PaCa-2 NWCx[VRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYOyNOKh|ryP MV[yJIg> NH\vPIV{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? NUfuTHl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
BxPC-3 NXK1[JF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPzNlDDqM7:TR?= MmfkNkBp NFewb|N{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
AsPC-1 NYLZeYJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXsRlYzOMLizszN M{TLNVIhcA>? NWexNY9Re2Wwc3n0bZpmeyClZXzsJIxqdmW|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On Mo\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
HEY Ml;GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWOyNOKh|ryP NH3PN5QzKGh? M2CxdZNmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? NUX2Z4h6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFg1QDlpPkK1PFg5PDh7PD;hQi=>
OVCAR-3 M1rsVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUmyNOKh|ryP MlyyNkBp Moqzd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm Mk\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
SK-OV-3 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fuRlIxyqEQvF2= M2m1cVIhcA>? NUD5WnJ4e2Wwc3n0bZpmeyClZXzsJIxqdmW|IITvJJRz\WG2bXXueEB4cXSqIEKtSGch[W6mIFStZYxtd3On M4rMb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
MH7A  MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLPNlQhcA>? MYLy[Ylv\m:{Y3XzJJRp\SCrbnjpZol1d3K7IHXm[oVkfHNib3[gXGFP NIry[m49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2Nlk3Pid-MkW4OlI6PjZ:L3G+
MH7A  MmfrRZBweHSxc3nzJGF{e2G7 MoDxNlUh|ryP MXWyOEBp Mo\TdoV3\XK|ZYOgZ4VtdCCleXPs[UBienKnc4SgbY5lfWOnZDDifUBZSU5iYX7kJINifXOnZDDhdI9xfG:|aYOgc4Yh[2WubIOgeoliKGGldHn2ZZRqd25ib3[gTm5M MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh4Mkm2Okc,OjV6NkK5OlY9N2F-
SCC25 NUfCfpZRTnWwY4Tpc44hSXO|YYm= Mlq1NlAh|ryP MXWyOEBp Mn7RbY5kemWjc3XzJIF2fG:yaHHnfUBt\X[nbB?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNEc,OjV6M{S0NFA9N2F-
HaCaT  M2nhVWZ2dmO2aX;uJGF{e2G7 MnPxOUDDvU1? M1T5RlI1KGh? NYXOcG1LcW6qaXLpeJMhUU[QLd8zMYlv\HWlZXSgR2NNOjJicILv[JVkfGmxbjDs[ZZmdHN? NV3RXlh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q{PTNpPkK1PFM1OzV|PD;hQi=>
HaCaT  NIe0RZpHfW6ldHnvckBCe3OjeR?= M4rxN|UhyrWP MWCyOEBp MnflbY5pcWKrdIOgTWZPNc7zLXnu[JVk\WRiQ1PMNlIheHKxZIXjeIlwdiCuZY\lcJM> MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6M{SzOVMoRjJ3OEO0N|U{RC:jPh?=
HPAEpiCs  M4\DO2Z2dmO2aX;uJGF{e2G7 MnXjNU8{NzFyIN88US=> Mm\rNUBp NXO0N5VGemWmdXPld{BUOVBvaX7keYNm\CCLQ1HNMVEheHKxdHXpckBidmRibWLORUBmgHC{ZYPzbY9vKGGwZDDwdo9ud3SncjDhZ5Rqfmm2eR?= MljvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs NHrE[XlHfW6ldHnvckBCe3OjeR?= M2XSN|EwOy9zMDFOwG0> MVWxJIg> NUDFOWU{cW6qaXLpeJMhWzGSLYP0bY12dGG2ZXSgRYt1KHCqb4PwbI9zgWyjdHnvckAhyqB? NFXwVXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUezOFkxOCd-MkW3N|Q6ODB:L3G+
HPAEpiCs MlKwSpVv[3Srb36gRZN{[Xl? NX[5SpJlOS9|L{GwJO69VQ>? MlvBNUBp M3i3NolvcGmkaYTzJHMyWCC2aX3lMYRmeGWwZHXueIx6KHO2aX31cIF1\WRiYz3KeY4heGixc4Doc5J6dGG2aX;u M2XsOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0PVAxLz5{NUezOFkxODxxYU6=
K562 MYnGeY5kfGmxbjDBd5NigQ>? MknFNVDDqM7:TR?= NU\LUZZqOcLiaB?= MnG2SG1UVw>? M1jqNYlvcGmkaYTzJJF2cW6jY4LpcoUucW6mdXPl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36= NXzsc5VSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFQxPDNpPkK1Olg1ODR|PD;hQi=>
PANC-1 NFrLbFJHfW6ldHnvckBCe3OjeR?= M{CwdlExKM7:TR?= NYnoVIlFOSCq NVHmXIMz\W6qYX7j[ZMhfGinIHH1eI9xcGGpaXOg[YZn\WO2wrC= MnjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{KyNlIoRjJ3NkOyNlIzRC:jPh?=
BxPC-3 M{XwZWZ2dmO2aX;uJGF{e2G7 Mn7BNVAh|ryP MX[xJIg> NGLrUlRmdmijbnPld{B1cGViYYX0c5Bp[WerYzDl[oZm[3UEoB?= NUX5OmppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|IzOjJpPkK1OlMzOjJ{PD;hQi=>
K562  M4i1fmZ2dmO2aX;uJGF{e2G7 MX[wMlI2NTFizszN NXv4VFlWOjRiaB?= NX;adnNve3WycILld5NmeyC{ZYP2[ZJifHKxbD3pcoR2[2WmIFiyRXgheGixc4Doc5J6dGG2aX;u MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzOUO5Nkc,OjV4MUmzPVI9N2F-
THP-1 NXHIPFMzTnWwY4Tpc44hSXO|YYm= NGLVdnA2KML3TR?= NGWwd5EzKGh? MkSxd4lodmmoaXPhcpRtgSCjdITlcpVifGW|IIPlZ5JmfGmxbjDv[kBKVC1zzsGgbY5lfWOnZDDifUAzP0:KQ3jvcEBxdHW|IF\TUE0yyqB? MnnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUi2OlEoRjJ3NUm4OlYyRC:jPh?=
WB MYHGeY5kfGmxbjDBd5NigQ>? M2jkflIx6oDLzszNxsA> M37hT|Mx6oDLbXnu MUjk[YNz\WG|ZYOgeIhmKEySUz2gc5IhVFSDLXnu[JVk\WRiSVytOkBidmRiVF7GMe6yyqCycn;keYN1cW:w M1T0ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUOwOlgzLz5{NUWzNFY5OjxxYU6=
RAW 264.7 NXvqSXZiTnWwY4Tpc44hSXO|YYm= M4nvOlExyqEQvF2= M16ye|Mx6oDLbXnu NFnRdWVqdmS3Y3XzJINp[XKjY4Tldol{fGmlII\hZ5VwdGG2aX;uJI9nKE:Fc9Mg NYPGPVJ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlE{QTlpPkK1OFYyOzl7PD;hQi=>
RAW 264.7 MWrGeY5kfGmxbjDBd5NigQ>? NHXMOFkyOMLizszN NYewNolROzEkgJntbY4> NIrq[INifHSnboXheIV{KHSqZTDl[oZm[3S|IH;mJG9RTyCxbjDvd5Rmd2OuYYP0JJJmfHKjY4Tpc44> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUO5PUc,OjV2NkGzPVk9N2F-
HaCaT  NWnZem12TnWwY4Tpc44hSXO|YYm= Mn6wOFDDqM7:TR?= NWC4dVMyOy1{NDDo NXXGUHNRTE2VTx?= NVzGPG5{emWmdXPld{B1cGViYXPjeY12dGG2aX;uJI9nKFqRLUG= NFHxeFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOVQ5PSd-MkW0N|U1QDV:L3G+
H9c2 NWHKTo9GTnWwY4Tpc44hSXO|YYm= NEPKb442OMLizszNxsA> NUnOPI9jOTJiaB?= M{PnXpJm\HWlZYOgUGRJKHKnbHXhd4Uh[W6mIF3NVEBtd3O| NET5ZXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK0OVgyQCd-MkWyOFU5OTh:L3G+
HSCs MWPBdI9xfG:|aYOgRZN{[Xl? MnXxNlUh|ryP NVXW[ZN3OjUEoHi= MmfQd4lodmmoaXPhcpRtgSCjdITlcpVifGW|IGTHMYlv\HWlZXSgZYN1cX[jdHXkJGhUS3NiYYDvdJRwe2m| M3fZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[xPVUxLz5{NEm2NVk2ODxxYU6=
THP-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF32SGQ4OiCq M3HSbGROW09? NXmxZWprUUN3ME20MlfPxE1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNUC4O{c,OjR6MUWwPFc9N2F-
MDDCs MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfXRnQ4OiCq MULEUXNQ MVPJR|UxOi55zszN MnXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs MmLoSpVv[3Srb36gRZN{[Xl? NYL6OZE4OC1zNTFOwG0> M3jqb|Q5KGh? NVi4Zo1LTE2VTx?= NE\TW25{fXCycnXzd4V{KEmITj5OtUBidmRiSWCtNVAheHKxZIXjeIlwdsLi MlXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs MXHGeY5kfGmxbjDBd5NigQ>? MUWwMVE2KM7:TR?= MWC0PEBp MYLEUXNQ MkjrbY5pcWKrdIOgUWlRNTGjLDDNTXAuOWJiYX7kJHJCVlSHUzDwdo9lfWO2aX;u MkPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MUWwPFcoRjJ2OEG1NFg4RC:jPh?=
MDDCs NI\Od4ZHfW6ldHnvckBCe3OjeR?= NYLrdY1iOTBizszN NFHme40{NjViaB?= NFSxWW5FVVOR NUTodFhJ[myxY3vzJGVDV1ZiR2CsJIJ2fCCwb4SgWnNXKEdibXXkbYF1\WRiZX70dpkhcW62bzDoeY1idiCPRFTDdy=> NXfjb2VKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
macrophages NF2yPHBHfW6ldHnvckBCe3OjeR?= MWqx5qCK|ryP Mn\1OE426oDLaB?= NIDieZhkd22ybHX0[Yx6KGmwaHnibZR{KE2WLVnJTU1qdmS3Y3XkJIFkfGm4YYTpc44hd2ZiTl[t{tpD MmXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6MEi2N|MoRjJ2OEC4OlM{RC:jPh?=
PDL  NXKwVYExTnWwY4Tpc44hSXO|YYm= MUKyNOKh|ryPwrC= M2XX[FMxKG2rbh?= NWjzcpRNTE2VTx?= NYfwfVU{e2mpbnnmbYNidnSueTDpcohq[mm2czD0bIUhfGWwc3ns[UBnd3KlZT3t[YRq[XSnZDDCUXAuOiCneIDy[ZN{cW:w NX3aU3RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OlExQDFpPkK0OVYyODhzPD;hQi=>
AGS NHP0e|ZHfW6ldHnvckBCe3OjeR?= NXvvSGdtPSEQvF2= NGe1[mY{OCCvaX6= MX7zeZBxemW|c3XzxsAyNWmwZIXj[YQh[2G|cHHz[U05KGGwZDDjZZNx[XOnLUOgZYN1cX[jdHnvci=> NEfEXY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW0O|g4QCd-MkS1OFc5Pzh:L3G+
H520 NULiR3hZTnWwY4Tpc44hSXO|YYm= MmfqNVDDqML3TR?= MkL6NUBp NYLtZ|RVTE2VTx?= M1XF[IRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV|MES3OUc,OjR3M{C0O|U9N2F-
H1703 MVzGeY5kfGmxbjDBd5NigQ>? MUmxNOKhyrWP NHfmb3IyKGh? NWPwOG5wTE2VTx?= M2KySYRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO= MoTHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{C0O|UoRjJ2NUOwOFc2RC:jPh?=
H520 NYCyd4JxTnWwY4Tpc44hSXO|YYm= Mlu1NVDDqML3TR?= NYfiWpo{OTJiaB?= M{TpR2ROW09? NF\ETldqdmirYnn0d{Bx\W2ndILlfIVlNWWuaXPpeIVlKE2VSEKgdJJwfGWrbjDzeIFjcWyrdIm= NV3vb|BiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703 M1z3TmZ2dmO2aX;uJGF{e2G7 MWWxNOKhyrWP Mle4NVIhcA>? MnvMSG1UVw>? Mn2zbY5pcWKrdIOgdIVu\XS{ZYjl[E1mdGmlaYTl[EBOW0h{IIDyc5RmcW5ic4ThZoltcXS7 MkLDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{C0O|UoRjJ2NUOwOFc2RC:jPh?=
H520 M1Xhc2Z2dmO2aX;uJGF{e2G7 NHnS[ogyOMLiwsXN Mo\tOkBp MmPrSG1UVw>? MX3zbYdvcW[rY3HueIx6KGmwY4LlZZNmeyC2aHWgcIV3\Wy|IH;mJJVjcXG3aYTpck1kd26sdXfheIVlKE2VSEKgbY4heGWvZYTy[Zhm\C22cnXheIVlyqClZXzsJIxqdmV? M3PPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
H1703 M1m3WmZ2dmO2aX;uJGF{e2G7 MYWxNOKhyrWP NIHHdng3KGh? NFX5cphFVVOR NYHuXGFOe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC3YnnxeYl1cW5vY3;ubpVo[XSnZDDNV2gzKGmwIIDlcYV1emW6ZXSteJJm[XSnZNMgZ4VtdCCuaX7l NGjvTmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzNFQ4PSd-MkS1N|A1PzV:L3G+
MC3T3-E1 Mn3ISpVv[3Srb36gRZN{[Xl? M{\HVlAvOy9|L{OwJO69VQ>? MWCxJIg> M{jheYF1fGWwdXH0[ZMhXE6ILd8xMYlv\HWlZXSgUW1RNTliZYjwdoV{e2mxbjDpckBiKGOxbnPlcpRz[XSrb36t[IVx\W6mZX70JI1idm6nctMg Mn;vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MEK2PVYoRjJ2NUCyOlk3RC:jPh?=
MC3T3-E1 NH\Y[YFHfW6ldHnvckBCe3OjeR?= M{O0[FMxKM7:TR?= NHW4OI0yKGh? MVvheJRmdnWjdHXzJHRPTi4QsT3zeIlufWyjdHXkJJA{QCCPQWDLJJBpd3OyaH;yfYxifGmxbh?= NUDpN2hXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFI3QTZpPkK0OVAzPjl4PD;hQi=>
HUVECs  MX;GeY5kfGmxbjDBd5NigQ>? NGnPSJkyOMLiwsXN MlvXNUBp NIjhd2xqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDDXGNNOSCycn;keYN1cW:w NVjmcoNuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PFc6PjRpPkK0OFg4QTZ2PD;hQi=>
AGS  M1jhRmZ2dmO2aX;uJGF{e2G7 NIXOXY4yOMLiwsXN NX:2e3JiOzEEoH3pci=> MYjpcohq[mm2czDJUE0y|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>? MknRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
MKN-45 MnHqSpVv[3Srb36gRZN{[Xl? MnTENVDDqML3TR?= MYSzNOKhdWmw NIDD[mVqdmirYnn0d{BKVC1zzsKtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB|OB?= NWCxTZlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
AGS  NHvq[ItHfW6ldHnvckBCe3OjeR?= NEDUSmoyOMLiwsXN M{e2bFMxyqCvaX6= NY\xZYFl[XS2ZX71ZZRmeyCLTD2x{tIucW6mdXPl[EBISSClZXzsJI1q\3KjdHnvckBidmRiaX72ZZNqd25? NEXtb|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O|k3QDF:L3G+
MKN-45 NVTjXFZ6TnWwY4Tpc44hSXO|YYm= M2TPVVExyqEEtV2= NWXpfoRlOzEEoH3pci=> M4L2foF1fGWwdXH0[ZMhUUxvMd8yMYlv\HWlZXSgS2Eh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9v NEL5VYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O|k3QDF:L3G+
AGS  MmDQSpVv[3Srb36gRZN{[Xl? MmLaNVDDqML3TR?= M4jDcVMxyqCvaX6= MoPJd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNiSXytNe6zNWmwZIXj[YQhVU2SMjDhcoQhVU2SOTDtVm5CKGW6cILld5Nqd25? NUnyNVl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45 MVPGeY5kfGmxbjDBd5NigQ>? NHvHcmYyOMLiwsXN M2n3N|MxyqCvaX6= NFT0Rlh{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDJcE0y|rJvaX7keYNm\CCPTWCyJIFv\CCPTWC5JI1TVkFiZYjwdoV{e2mxbh?= NYTaTo81RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
DCs NFT5PJJHfW6ldHnvckBCe3OjeR?= M4TxRVIxyqEQvF2= M3LxUFHDqGh? NIDUZWNl\WO{ZXHz[ZMhUUxvMUKgdJJw\HWldHnvci=> NXX5do1QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0N|Q3OzZpPkK0OFM1PjN4PD;hQi=>
HUVEC  MmLpSpVv[3Srb36gRZN{[Xl? NH;4NowzOCEQvH5CpC=> NGXHdGg2KGh? NHOydYxFVVOR NXL6dos4emWmdXPld{BkgXSxa3nu[UBmgHC{ZYPzbY9vKGyndnXsd{BqdiCjIHPvcoNmdnS{YYTpc44u\GWyZX7k[Y51KG2jbn7ldi=> NV\G[YV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPFkxPjJpPkK0NVg6ODZ{PD;hQi=>
A 549 MXfGeY5kfGmxbjDBd5NigQ>? MnHGOVAh|ryP NYrjXpZqOSCq MYPk[YNz\WG|ZYOgeIhmKGyndnXsJI9nKEmOLUlCpC=> MkThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{m2PFgoRjJ2MUe5Olg5RC:jPh?=
H520  MWjGeY5kfGmxbjDBd5NigQ>? M2HSTVUwOTBizszN M{SxfVEhcA>? NH\WOYxFVVOR Mm\n[IVkemWjc3XzJG1UUDJicILveIVqdiCjczD3[YxtKGG|IH3SUmEhdGW4ZXzzJIlvKGenZnn0bY5q[i2neIDvd4VlKGOnbHy= M4Tkd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
H1703  M1OyPWZ2dmO2aX;uJGF{e2G7 MYi1M|ExKM7:TR?= MXixJIg> MnXJSG1UVw>? Mlzh[IVkemWjc3XzJG1UUDJicILveIVqdiCjczD3[YxtKGG|IH3SUmEhdGW4ZXzzJIlvKGenZnn0bY5q[i2neIDvd4VlKGOnbHy= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|OEmwN{c,OjRzM{i5NFM9N2F-
H520  NYHhWI1jTnWwY4Tpc44hSXO|YYm= MYixNOKhyrWP MlTkNVIhcA>? NXTPc5FUTE2VTx?= NUfldng{\GWlcnXhd4V{KE2VSEKgcXJPSSCjbnSgdJJwfGWrbjDzeIFjcWyrdImgbY4h\2WoaYTpcoljNXS{ZXH0[YQhVlOFTFOgZ4VtdHN? M3v4N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
H1703  M17SUGZ2dmO2aX;uJGF{e2G7 MUGxNOKhyrWP MnvDNVIhcA>? MnrqSG1UVw>? Mnfl[IVkemWjc3XzJG1UUDJibWLORUBidmRicILveIVqdiC|dHHibYxqfHliaX6g[4VncXSrbnniMZRz\WG2ZXSgUnNEVENiY3XscJM> MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|OEmwN{c,OjRzM{i5NFM9N2F-
MCF-7  M4XtUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlS1NVDDqM7:TR?= NIXzS|QzPMLiaB?= NWDmflAycW6qaXLpeJMhfGinIFPSNVA5NWmwZIXj[YQh[2WubDDk[YF1cA>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{OEi1N{c,OjRzMki4OVM9N2F-
HPAEpiCs NXfMZpVTTnWwY4Tpc44hSXO|YYm= M4XTZ|AvOS9zL{GwJO69VQ>? M1HOcFEhcA>? NV[xTFJkcW6qaXLpeJMhXE6ILd8xMYlv\HWlZXSgZ3BNSTMEoIDyc5RmcW5iYX7kJI1TVkFiZYjwdoV{e2mxbh?= NFHCcnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2PVE2QCd-MkSwOlkyPTh:L3G+
podocytes NWL0eFFlTnWwY4Tpc44hSXO|YYm= M1nKXFExyqEQvF2= M{XiN|EhcA>? MUfpcohq[mm2czDUS2bPujFvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFugZY5lKEW{a{GvNuKh NIOwSpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECzOlIyOid-MkSwN|YzOTJ:L3G+
MCF-7 NInHS5FHfW6ldHnvckBCe3OjeR?= MkC1NVDDqM7:TR?= M3nEPVEhcA>? M2n5T2ROW09? M{HPZZJm\HWlZYOgeIhmKFeDLXnu[JVk\WRicHjvd5Bpd3K7bHH0[YQheDN6IF3BVGs> Mo\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUmwPVAoRjJ2MEG5NFkxRC:jPh?=
MCF-7 M3y1fWZ2dmO2aX;uJGF{e2G7 MmrqNVDDqM7:TR?= M2THNVI1KGh? NEPMUm1FVVOR NGq4eJNqdmO{ZXHz[ZMhfGinIGfBMYlv\HWlZXSgZZBweHSxc3nz NV7qWYIxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkxQTBpPkK0NFE6ODlyPD;hQi=>
HAPI M{PpXWZ2dmO2aX;uJGF{e2G7 M2S3ZVExNzJyL{SwxsDPxE1? Mn3kNUBp NGTSZ2dqdmirYnn0d{BVS0SGLXnu[JVk\WRicEO4M2pPUyCPQWDLJJBpd3OyaH;yfYxifGmxbh?= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OUGyNEc,OjN7NkmxNlA9N2F-
HAPI MnPwSpVv[3Srb36gRZN{[Xl? NHvadGUzOMLizszNxsA> M1LvWVEhcA>? NGDvfG5FVVOR NYLqS4JD[XS2ZX71ZZRmeyCWQ1TEMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCrTl;TJIFv\CCycn;keYN1cW:w MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OUGyNEc,OjN7NkmxNlA9N2F-
HepG2 NIHvZ3VHfW6ldHnvckBCe3OjeR?= MUGzOVAhdk1? NFf2XpozPCCq NInVfGVqdmirYnn0d{B1cGViZHXneYVtcW5vaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFu= M3PzSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUOzNVk5Lz5{M{mzN|E6QDxxYU6=
AGS  M3XQO2Z2dmO2aX;uJGF{e2G7 MnvENVDDqM7:TR?= NYjqVHNrOzBibXnu MX;pcohq[mm2czDjZZNx[XOnLUOgZYN1cX[jdHnvckBidmRiaX7obYJqfGmxbjDv[kBGWkt? NYHUW3hlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OVA6QTRpPkKzPFUxQTl2PD;hQi=>
HepG2 M4jURmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXDNE02OCEQvF2= Mlj1OFghcA>? NIflXlRqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> M1v6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC3OVA5Lz5{M{iwO|UxQDxxYU6=
BEL7404 M{K1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnv3NE02OCEQvF2= MmntOFghcA>? NHLvTGlqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> NIjm[IQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO|UxQCd-MkO4NFc2ODh:L3G+
HL7702 NF:4SlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUP6N3BlOC13MDFOwG0> NInlZ3A1QCCq NYXISVhucW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> M{TJbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC3OVA5Lz5{M{iwO|UxQDxxYU6=
HepG2 NF7Od3dHfW6ldHnvckBCe3OjeR?= NGH1[VUxNTVyIN88US=> NYL0VHZ5OjRiaB?= MVrpcohq[mm2czD0bIUheGixc4Doc5J6dGG2aX;uJI9nKHB|ODDkc5dve3S{ZXHtJJBzd3SnaX7zJG1CWEuDUFuyMEBCXEZ{LDDNV2syKGGwZDDIV3AzPyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyN{WwPEc,OjN6MEe1NFg9N2F-
BEAS-2B M3T3WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jW[|ExKM7:TR?= MoGyN|AhdWmw NYXWdmtZemW4ZYLz[ZMhfGinIHTlZ5Jm[XOnIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgTGNK MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN|Q9N2F-
BEAS-2B NVy0[XZSS3m2b4TvfIl1gSCDc4PhfS=> M{TlfFExKM7:TR?= MV[zNEBucW5? Ml3lbY5pcWKrdHXkJJRp\SCrbnPy[YF{\SCrbjDMSGgh[W6mIFnMMVgh\XiycnXzd4lwdg>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN|Q9N2F-
BEAS-2B MUDGeY5kfGmxbjDBd5NigQ>? MnnFNVAh|ryP MVuzNEBucW5? NFjQZWFl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDjZZNx[XOnLUOsJGJi\CCjbnSg[oF{ NXLObVl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PFQxOzRpPkKzO|g1ODN2PD;hQi=>
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U937 MX\GeY5kfGmxbjDBd5NigQ>? M3\LXVExyqEQvF5CpC=> NH\nR2UyKGh? MVfhZpJw\2G2ZYOgZ4Fn\mWrbnWtbY5lfWOnZDDNT3AuOSCmb4fuMZJm\3WuYYTpc44h[W6mIGDQNmFk|rFidYCtdoVofWyjdHnvci=> NIn4N5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|M5Pyd-MkO3NFc{QDd:L3G+
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A549 M4LmNWZ2dmO2aX;uJGF{e2G7 Mn[2NVDDqM7:TdMg M2Dp[VAuOzBibXnu M{DLZolvcGmkaYTzJGFVWM7|UzDpcoR2[2WmIIC0Nk9xPDRiTVHQT{BidmRicEO4JG1CWEticHjvd5Bpd3K7bHH0bY9v NV64Xoo3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549 MnjrSpVv[3Srb36gRZN{[Xl? NUP2ZnlLOTEEoN88UeKh M2jxWFEhcA>? NHO3ZmNqdmirYnn0d{BCXFEQs2OgbY5lfWOnZDDOSk3PwkJicE[1JJN2[nWwaYSgdIhwe3Cqb4L5cIF1cW:wIHHu[EBPTi4QulKgdJJwdW:2ZYKgZYN1cX[rdIm= Ml\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEC2O|QoRjJ|NkiwOlc1RC:jPh?=
A549 M2H0[GZ2dmO2aX;uJGF{e2G7 MYexNOKh|ryPwrC= M4HpblEhcA>? M1f5O5Jm\HWlZYOgRXRR|rOVLYP0bY12dGG2ZXSgZ3BNSTJicHjvd5Bpd3K7bHH0bY9vyqB? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6ME[3OEc,OjN4OEC2O|Q9N2F-
A549 NVnOSGZWTnWwY4Tpc44hSXO|YYm= NFnZXogyOMLizszNxsA> NYTSd4w4OSCq NFLKVY1z\WS3Y3XzJGFVWM7|Uz3lcohidmOnZDDlcpp6dWG2aXOgZYN1cX[rdImgc4Yh[1COQUNCpC=> NUXGRoFpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
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HK-2 MUnBdI9xfG:|aYOgRZN{[Xl? NHHWeVAzOMLizszN NEjYZVEzPCCq NH\3d4VqdmirYnn0d{BGWktiYX7kJJA{QE2DUFu= MkHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEOxOVEoRjJ|NUSzNVUyRC:jPh?=
H9c2 NHzxNFVHfW6ldHnvckBCe3OjeR?= M{G0ZVHDqM7:TR?= NWj3RVRvOSCq NHLUPJVz\WS3Y3XzJHRPTi4QsT3pcoR2[2WmIF3NVE06KG2UTlGgcIV3\Wy|IHHu[EBxem:vb4TldkBi[3Srdnn0fS=> M4PQUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{WzOlk6Lz5{M{O1N|Y6QTxxYU6=
H9c2 NIPBZnRHfW6ldHnvckBCe3OjeR?= NXXpVolJOcLizszN NYizU4RbOSCq M4rvb5Jm\HWlZYOgWG5HNc7zLXXubIFv[2WmIFHQMVEheHKxbX;0[ZIh[WO2aY\peJk> MnTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NUO2PVkoRjJ|M{WzOlk6RC:jPh?=
H1650  MV3GeY5kfGmxbjDBd5NigQ>? NVnWU3dsOTEEoN88UeKh MXexJIg> MVTEUXNQ M1fnS4Rm[3KnYYPld{Bjd3SqIIDyc5RmcW5iYX7kJI1TVkFibHX2[Yx{KG:oIFXSR2MyKGmwIIDhZ4xqfGG6ZXyt[Zhxd3OnZNMgZ4VtdHN? M4\0cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkK4Olk3Lz5{M{KyPFY6PjxxYU6=
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medulloblastoma cells MYLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4rMXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUA{NjByNjFOwG0v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N|E9N2F-
neural precursor cells NIPV[pdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkhVVSWIHHzd4F6NCCHQ{WwJF0hQC5yNkOg{txONg>? MmrTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUe2N|EoRjF5NEG3OlMyRC:jPh?=
RAW264.7 NUK0NoZjSW62aXnu[oxidW2jdH;yfUBie3OjeR?= MUexNEBucW6| NE[4WXRCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbnP1ZoF1\WRiZn;yJFExKG2rboOgdJJqd3JidH:gUHBUNWOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAyQCCqcoOgZpkhT3KrZYPzJI1mfGixZDygTWM2OCB;IEG2JO69VS5? MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6M{G3PVgoRjJ{OEOxO|k5RC:jPh?=
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RAW264.7 NHrxcWpHfW6ldHnvckBie3OjeR?= NIrHbmlRem:2ZXP0bY9vKGGpYXnud5QhSmGlaXzseZMh[W62aILhZ4l{KHC{b4TlZ5RqfmViYX70bYdmdiCjbnSgcIV1cGGuIITvfIlvNWSrcHj0bIVzcWFidH;4bY4h[2irbXXybYMheHKxdHXpckBu\WSrYYTl[EBkgXSxdH;4bYNqfHliaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJk> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR6NUWwOEc,OTd2OEW1NFQ9N2F-
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NB1643 NHzGSldyUFSVIHHzd4F6 MnzxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MkPoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-12 NF[zUGNyUFSVIHHzd4F6 NIPxUXZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1zMjDj[Yxtew>? NF\YTIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 M1\Ye5FJXFNiYYPzZZk> M3LzR5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGxCVi13IHPlcIx{ NVzwTHVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 NHewc2ZyUFSVIHHzd4F6 MVzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? NV;Gb5dHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1 M{e5b5FJXFNiYYPzZZk> M4D2RpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DNUWEY{GgZ4VtdHN? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH MYTxTHRUKGG|c3H5 MkPYdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2VSDDj[Yxtew>? NVi5eWpWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673 MUDxTHRUKGG|c3H5 NEnaSmRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? Mof4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 MlnIdWhVWyCjc4PhfS=> M3XFRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTN5IHPlcIx{ MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) M2SxSpFJXFNiYYPzZZk> NEPSdGtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBOTyB4MzCoOk1VTyCUKTDj[Yxtew>? Ml;JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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OHS-50 MkX4dWhVWyCjc4PhfS=> MlHLdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgU2hUNTVyIHPlcIx{ MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MlPpdWhVWyCjc4PhfS=> NGroRm9yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDRzIHPlcIx{ NEHpRpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC M36zUpFJXFNiYYPzZZk> M1\zepFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz M2DLOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MnvNdWhVWyCjc4PhfS=> MXzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORlE3PDNiY3XscJM> MnjGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC M2n4SZFJXFNiYYPzZZk> NIHMc|lyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 NYW4SZAzeUiWUzDhd5NigQ>? NFTuW|hyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| M1PVd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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Assay
Methods Test Index PMID
Western blot mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2 ; pJNK1 / pJNK2 / JNK1 / JNK2 26844273 18222647
Immunofluorescence p-p38 / MMP9 24479681
Growth inhibition assay Cell viability 26844273
In vivo

Inhibiting p38 by administration of SB 202190 inhibits PV IgG-induced blister formation in the passive transfer mouse model. [5] In the endotoxin model of sepsis, SB 202190 treatment produces a statistically significant survival benefit compared with control. [8]

Protocol (from reference)

Kinase Assay:

[1]

  • In vitro kinase assays:

    The p38α and p38β are assayed in 25 mM Tris-HCl, pH 7.5, containing 0.1 mM EGTA, with myelin basic protein (0.33 mg/mL) as substrate. Assays are performed either manually for 10 minutes at 30 °C in 50 μL incubations using [γ-33P]ATP, or with a Biomek 2000 Laboratory Automation Workstation in a 96-well format for 40 minutes at ambient temperature in 25 μL incubations using [γ-33P]ATP. The concentrations of ATP and magnesium acetate are 0.1 mM and 10 mM respectively. All assays are initiated with MgATP. Manual assays are terminated by spotting aliquots of incubation on to phosphocellulose paper, followed by immersion in 50 mM phosphoric acid. Robotic assays are terminated by the addition of 5 μL of 0.5 M phosphoric acid before spotting aliquots on to P30 filter mats. All papers are then washed four times in 50 mM phosphoric acid to remove ATP, once in acetone (manual incubations) or methanol (robotic incubations), and then dried and counted for radioactivity.

Cell Research:

[2]

  • Cell lines: Jurkat, and HeLa
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24 hours
  • Method:

    Cells are serum-starved and then treated with different concentration of SB 202190 for 24 hours. Cell viability is assayed by either trypan blue exclusion or propidium iodide exclusion followed by flow cytometry analysis. The apoptotic nuclei are visualized by H33258 staining.

Animal Research:

[5]

  • Animal Models: C57BL/6J mice injected i.d. with a sterile solution of either control IgG or PV IgG
  • Dosages: 12.5 μg
  • Administration: Administered via i.d.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%H2O
For best results, use promptly after mixing.

8 mg/mL

Chemical Information

Molecular Weight 331.34
Formula

C20H14N3OF

CAS No. 152121-30-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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