Y-39983 HCl

For research use only.

Catalog No.S7935 Synonyms: Y-33075 HCl

3 publications

Y-39983 HCl Chemical Structure

CAS No. 173897-44-4

Y-39983 HCl (Y-33075) is a selective rho-associated protein kinase(ROCK) inhibitor with an IC50 of 3.6 nM.

Selleck's Y-39983 HCl has been cited by 3 publications

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Biological Activity

Description Y-39983 HCl (Y-33075) is a selective rho-associated protein kinase(ROCK) inhibitor with an IC50 of 3.6 nM.
ROCK [1]
(Cell-free assay)
3.6 nM
In vitro

Y-39983 opposes ROCK-dependent phosphorylation of MYPT1 predominantly at Thr853 with a corresponding decrease in MLC phosphorylation. It reduces the phosphorylation at Thr696 and Thr853 and leads to reduced actomyosin contraction. Y-39983 shows an IC50 of ~200 nM for dephosphorylation at Thr696 and only 15 nM for dephosphorylation at Thr853. The IC50 for dephosphorylation of MLC is 14 nM[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
TM1 MXzGeY5kfGmxbjDhd5NigQ>? NYraW2RITGm|coXweIlwdiCxZjD0doFj\WO3bHHyJI1me2i5b4LrJIlvKGi3bXHuJHROOSClZXzsd{Bie3Onc4Pl[EBieyCjY4TpckBncWKncjDs[Y5ofGhiYX7kJIZw[2GuIHHkbIV{cW:wczDifUBJd2WlaIP0JJN1[WmwaX7nJIJie2WmIHHuZYx6e2m|LDDJR|UxKD1iMD6yO|gh|ryPLh?= NYi2Sm9iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewO|I6ODVpPkK3NFczQTB3PD;hQi=>
GTM3 NHzGcFFHfW6ldHnvckBie3OjeR?= MV:yJIRigXN? NX;mNm5FUW6qaXLpeIlwdiCxZjDSU2NMNTJiaX6gbJVu[W5iR2TNN{Bk\WyuczDt[YF{fXKnZDD1dEB1dyB{IHThfZMh[nliY3XscEBqdXCnZHHuZ4Uh[XO|YYmsJGVEPTBiPTCwMlE2KM7:TT6= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ6NEi0N{c,OjR4OES4OFM9N2F-
GTM3 Ml2xSpVv[3Srb36gZZN{[Xl? Mk\wTY5pcWKrdHnvckBw\iCUT1PLNUBqdiCqdX3hckBIXE1|IHPlcIx{KGK7IHntdIVl[W6lZTDhd5NigSxiRVO1NEA:KDBwN{mg{txONg>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR|NEOzOEc,OjB2M{SzN|Q9N2F-
SJ-GBM2 NGnoNHZyUFSVIHHzd4F6 M4[4TpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz NGfkVo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 NFHsOm9yUFSVIHHzd4F6 NWLK[YZIeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= NX;1dpd3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NEH1NVNyUFSVIHHzd4F6 Ml\KdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= MoTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 MoDWdWhVWyCjc4PhfS=> MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| NV[yNot1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH Ml:0dWhVWyCjc4PhfS=> NFn0VYRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> M2DuZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 M4\ZRpFJXFNiYYPzZZk> MV\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NI[zbmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-MYPT1 / ppMLC ; 

PubMed: 21850162     

Treatment with ROCK inhibitors completely opposed the phosphorylation of MYPT1 at Thr853. There was a concomitant decrease in the phosphorylation of MLC. However, the phosphorylation of MYPT1 at Thr696 was only slightly reduced in the presence of Y-27632 compared to Y-39983. 

F-actin / ppMLC ; 

PubMed: 21850162     

In the presence of ROCK inhibitors, there was a loss in stress fibers, and the staining for MLC appeared diffuse.

Growth inhibition assay
Cell viability; 

PubMed: 27487152     

Dose-response curve showing cell viabilities (as analysed by MTT assay) of hTERT-HME1 and MDA-MB-231 cells following 48 hr treatment with a range of concentrations of the ROCK inhibitor Y39983. IC50 values for both cell lines are shown.

In vivo Y-39983 significantly suppresses clinical symptoms of EAE and prevents its relapse while increasing the amount of myelin proteins. Its treatment results in reduced demyelination with no significant change in axonal damage, which may be due to the inactivation of ROCK substrates, including phosphorylated (p)-MLC, LIMK2 and CRMP-2, which are important for neurite outgrowth, growth cone collapse and remyelination of oligodendrocytes[2].


Cell Research:[3]
- Collapse
  • Cell lines: Human glaucomatous trabecular meshwork (GTM3) cell line
  • Concentrations: 5 μM
  • Incubation Time: 1 h
  • Method: Cell cultures of an immortalized human glaucomatous trabecular meshwork (GTM3) cell line are grown at 37℃ in 5% CO2 in Dulbecco's Minimum Essential Medium (DMEM + Glutamax) supplemented with 10% fetal bovine serum and 10 μg/ml of gentamicin. Upon reaching confluence, cells are divided using 0.05% trypsin. Cells of passages 13 to 20 are used in experiments. Cells are treated with 5 μM of the specific ROCK inhibitors, Y-27632 and Y-39983, for 1 h in a serum-rich medium. Cell lysate is used for western blotting to test effect of ROCK inhibitors on the phosphorylation of MYPT1 and MLC.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: SJL/J mice
  • Dosages: 100 μl of 10 μM
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 63 mg/mL (178.34 mM)
Water 63 mg/mL (178.34 mM)
Ethanol '2 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 353.25


CAS No. 173897-44-4
Storage powder
in solvent
Synonyms Y-33075 HCl
Smiles CC(C1=CC=C(C=C1)C(=O)NC2=C3C=CNC3=NC=C2)N.Cl.Cl

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID