Thiazovivin

For research use only. Not for use in humans.

Catalog No.S1459

31 publications

Thiazovivin Chemical Structure

Molecular Weight(MW): 311.36

Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 150 In stock
USD 120 In stock
USD 210 In stock
USD 670 In stock
Bulk Discount
Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Thiazovivin has been cited by 31 publications

Purity & Quality Control

Choose Selective ROCK Inhibitors

Biological Activity

Description Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.
Targets
ROCK [1]
(Cell-free assay)
~0.5 μM
In vitro

Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SCU-i10 hiPSCs M2Xsbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUKwMVIxKM7:TR?= MonwNlQhcA>? MUHzbYdvcW[rY3HueIx6KG2xZIXsZZRmeyC|aX7ncIUu[2WubDDwcIF1cW6pIHXm[olkcWWwY4mgZY5lKHC{b33veIUhVkOPIHfyc5d1cA>? MXGyOVA4Pzl|Mh?=
HSMCs Mk\jSpVv[3Srb36gRZN{[Xl? M2TZZ|HjiIoQvF2= MVKyJIg> NWLHWnpMemW4ZYLz[ZMhSW6pSVmtbY5lfWOnZDDNXXBVOSCyaH;zdIhwenmuYYTpc44> MmrpNlQ6PjVzN{C=

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
ROCK1/2; 

PubMed: 28849097     


Protein expression of ROCK1/2 in HCECs cultured with 4 µM TZV for different periods of time, n=3. HCECs, human corneal endothelial cells; TZV, thiazovivin.

28849097
Immunofluorescence
E-cadherin / N-cadherin / α-SMA; 

PubMed: 28849097     


Function-related protein expression in primary HCECs cultured with various concentrations of TZV for 24 h, n=3. (A) E-cadherin, N-cadherin, and α-SMA immunostaining (magnification, ×200);

28849097

Protocol

Solubility (25°C)

In vitro DMSO 15 mg/mL (48.17 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing. 		30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 311.36
Formula

C15H13N5OS
CAS No. 1226056-71-8
Storage powder
in solvent
Synonyms N/A
Smiles O=C(NCC1=CC=CC=C1)C2=CSC(=N2)NC3=CC=NC=N3

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
selleck catalog

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

  • Selective ROCK Inhibitor

    Fasudil (HA-1077) HCl : ROCK-II-selective, Ki=0.33 μM.

  • Most Potent ROCK Inhibitor

    GSK429286A : ROCK1, IC50=14 nM; ROCK2, IC50=63 nM.

  • ROCK Inhibitor in Clinical Trial

    Fasudil (HA-1077) HCl : Phase III for scleroderma.

  • Newest ROCK Inhibitor

    RKI-1447 : Highly potent inhibitor of?b>ROCK1燼nd?b>ROCK2, with?b>IC50爋f 14.5 nM and 6.2 nM, respectively.

Related ROCK Products

  • Ro-3306 New

    RO-3306 is an ATP-competitive, and selective CDK1 inhibitor with Ki of 20 nM, >15-fold selectivity against a diverse panel of human kinases.

  • CCG-1423 New

    CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

  • ML141 New

    ML141 (CID-2950007), is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7).

  • Y-27632 2HCl

    Y-27632 2HCl is a selective ROCK1 (p160ROCK) inhibitor with Ki of 140 nM in a cell-free assay, exhibits >200-fold selectivity over other kinases, including PKC, cAMP-dependent protein kinase, MLCK and PAK.

  • GSK429286A

    GSK429286A is a selective inhibitor of ROCK1 and ROCK2 with IC50 of 14 nM and 63 nM, respectively.

    Features:Greater selectivity than Y-27632.

  • Fasudil (HA-1077) HCl

    Fasudil(HA-1077), a potent and selective inhibitor of Rho kinase, displays less potent inhibiton over PKA, PKG, PKC and MLCK with Ki of 1.6, 1.6, 3.3, and 36 μM in cell-free assays, respectively.

  • RKI-1447

    RKI-1447 is a potent inhibitor of ROCK1 and ROCK2, with IC50 of 14.5 nM and 6.2 nM, respectively, has anti-invasive and antitumor activities.

  • Palbociclib (PD-0332991) HCl

    Palbociclib (PD-0332991) HCl is a highly selective inhibitor of CDK4/6 with IC50 of 11 nM/16 nM in cell-free assays, respectively. It shows no activity against CDK1/2/5, EGFR, FGFR, PDGFR, InsR, etc. Phase 3.

    Features:Non-cytotoxic, and halts cancer cell growth & could be used in glioblastoma that has relapsed after temozolomide treatment (a chemotherapeutic used to treat many cancers).

  • Alisertib (MLN8237)

    Alisertib (MLN8237) is a selective Aurora A inhibitor with IC50 of 1.2 nM in a cell-free assay. It has >200-fold higher selectivity for Aurora A than Aurora B. Phase 3.

    Features:First orally available inhibitor of Aurora A.

  • Adavosertib （MK-1775）

    MK-1775 is a potent and selective Wee1 inhibitor with IC50 of 5.2 nM in a cell-free assay; hinders G2 DNA damage checkpoint. Phase 2.

    Features:The first reported Wee1 inhibitor.

Tags: buy Thiazovivin | Thiazovivin supplier | purchase Thiazovivin | Thiazovivin cost | Thiazovivin manufacturer | order Thiazovivin | Thiazovivin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID