For research use only.

Catalog No.S1459

34 publications

Thiazovivin Chemical Structure

CAS No. 1226056-71-8

Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.

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Selleck's Thiazovivin has been cited by 34 publications

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Description Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.
ROCK [1]
(Cell-free assay)
~0.5 μM
In vitro

Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SCU-i10 hiPSCs MlHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrtNE0zOCEQvF2= M4jVPVI1KGh? NXPYbm16e2mpbnnmbYNidnSueTDtc4R2dGG2ZYOgd4lv\2ynLXPlcIwheGyjdHnu[{Bm\m[rY3nlcoN6KGGwZDDwdo9ud3SnIF7DUUBoem:5dHi= M3nRTVI2ODd5OUOy
HSMCs M3fzfWZ2dmO2aX;uJGF{e2G7 MkG0NgKBkc7:TR?= MlHVNkBp MnG4doV3\XK|ZYOgRY5oUUlvaX7keYNm\CCPWWDUNUBxcG:|cHjvdplt[XSrb36= NGnMNFIzPDl4NUG3NC=>

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Methods Test Index PMID
Western blot

PubMed: 28849097     

Protein expression of ROCK1/2 in HCECs cultured with 4 µM TZV for different periods of time, n=3. HCECs, human corneal endothelial cells; TZV, thiazovivin.

E-cadherin / N-cadherin / α-SMA; 

PubMed: 28849097     

Function-related protein expression in primary HCECs cultured with various concentrations of TZV for 24 h, n=3. (A) E-cadherin, N-cadherin, and α-SMA immunostaining (magnification, ×200);



Solubility (25°C)

In vitro DMSO 15 mg/mL (48.17 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 311.36


CAS No. 1226056-71-8
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)CNC(=O)C2=CSC(=N2)NC3=NC=NC=C3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID