Tetrandrine

Catalog No.S2403 Synonyms: Fanchinine

Tetrandrine Chemical Structure

Molecular Weight(MW): 622.75

Tetrandrine, a bis-benzylisoquinoline alkaloid derived from Stephania tetrandra, is a calcium channel blocker.

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Cited by 4 Publications

2 Customer Reviews

  • BMMs were seeded in a collage I-coated plate and incubated with RANKL (100 ng/ml) and M-CSF (50 ng/ml) for 5 d. When mature osteoclasts were observed, they were digested by collagenase and seeded on bovine bone slices. The bone slices were treated with or without tetrandrine for an additional 2 d. Then, the cells were stained with rhodamine–phalloidin to observe the F-actin rings, and the areas of F-actin rings were calculated by Image-Pro Plus 6.0 software.

    FASEB J, 2018, 32(6):3398-3410. Tetrandrine purchased from Selleck.

    (B) Measurement of the cellular LDs in 3T3-L1 preadipocytes (undifferentiated) or differentiated adipocytes on day 8 by Oil Red O staining. Phase-contrast images of the cells were also taken after the treatment (lower panels in B). Each picture in (B) is a representative of three independent experiments. (C) Quantification of the cellular TG contents in tetrandrine-treated 3T3-L1 preadipocytes on day 8 by AdipoRed assay. Values are mean ± SE of data from three independent experiments with three replicates. *P < 0.05 vs.control (no chemical). (D) 3T3-L1 preadipocytes were grown under the above-mentioned 3T3-L1 preadipocyte differentiation condition in Figure 1A. On day 8, tetrandrine-treated 3T3-L1 preadipocytes, which cannot be stained with trypan blue dye, was counted under microscope. The cell count assay was done in triplicates. Data are mean ± SE of three independent experiments. *P < 0.05 vs. control (no chemical).

    Biochemical and Biophysical Research Communications, 2016, 476(4):481-486.. Tetrandrine purchased from Selleck.

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Biological Activity

Description Tetrandrine, a bis-benzylisoquinoline alkaloid derived from Stephania tetrandra, is a calcium channel blocker.
Targets
Calcium channel [1]
In vitro

Tetrandrine inhibits voltage-gated Ca2+ currents with an IC50 of 10.1 mM in isolated nerve terminals of the rat neurohypophysis. Tetrandrine is a high-affinity blocker of the type II, maxi-Ca(2+)-activated K+ channel of the rat neurohypophysial terminals with an IC50 of 0.21 mM. [1] Another study shows that Tetrandrine also inhibits Ca(2+)-activated Cl- currents (I(Cl,Ca)) with an IC50 of 5.2 mM. [2] Tetrandrine inhibits the proliferation of human leukemic HL-60 cells via induction of apoptosis. [3]

Assay
Methods Test Index PMID
Western blot
p-GSK3β / GSK3β / p-β-catenin / β-catenin ; 

PubMed: 29334999     


Western blot analysis of p-GSK3β, total GSK3β, p-β-catenin, and total β-catenin protein levels after Huh7 and HCCLM9 cells were treated with DMSO or 2-μM tetrandrine (Tet) for 24 h.

Atg7; 

PubMed: 22927446     


Western blot of ATG7 in cells treated with 5 μm tetrandrine for the indicated times.

p-ASK1 / ASK1 / p-SEK1 / SEK1 / p-JNK1/2 / JNK1/2 ; 

PubMed: 25181458     


PC3 and DU145 cells were treated with tetrandrine (15 μM) for 0, 6, 12, 18, and 24 h at 37°C. Total protein lysates (25 μg) were analyzed by Western blotting for the expression of proteins indicated in the panel. GAPDH was used as a loading control. 

29334999 22927446 25181458
Growth inhibition assay
Cell viability; 

PubMed: 29285984     


The maximum non-cytotoxic dose of tetrandrine in CNE1 and CNE2 cells. The data shown are the mean and SE from three independent experiments.*p < 0.05 vs control.

29285984
Immunofluorescence
E-cadherin / Vimentin ; 

PubMed: 29334999     


Huh7 and HCCLM9 cells were treated with 0 (DMSO) or 2-μM tetrandrine (Tet) for 24 h and then subjected to immunofluorescence analysis of the E-cadherin and vimentin levels. Scale bars: 20 μm.

p-STAT3; 

PubMed: 28332288     


The effect of tetrandrine on the nuclear translocation of STAT3. The cells were fixed and then subjected immunofluorescence assay with mouse p‐STAT3 antibody (upper column), and the images were superimposed with DAPI-stained images (middle column) and merged images (lower column). The representative photographs from the each group are shown. Scale bar, 50 μm.

29334999 28332288
In vivo LD50: Mice 158.9mg/kg (i.v.) [4], 365mg/kg (i.p.) [5]; Rats 646mg/kg (i.g.) [6]

Protocol

Solubility (25°C)

In vitro Ethanol 2 mg/mL (3.21 mM)
DMSO 1 mg/mL warmed (1.6 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 622.75
Formula

C38H42N2O6

CAS No. 518-34-3
Storage powder
in solvent
Synonyms Fanchinine

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID