Imperatorin

Catalog No.S3809 Synonyms: Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen, 8-Isopentenyloxypsoralene

For research use only.

Imperatorin (Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen), a naturally occurring psoralen-type furanocoumarin, is a potent myorelaxant agent and acts as a calcium antagonist on vascular smooth muscle.

Imperatorin Chemical Structure

CAS No. 482-44-0

Selleck's Imperatorin has been cited by 3 Publications

Purity & Quality Control

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Biological Activity

Description Imperatorin (Ammidin, Marmelosin, Pentosalen, 8-Isoamylenoxypsoralen), a naturally occurring psoralen-type furanocoumarin, is a potent myorelaxant agent and acts as a calcium antagonist on vascular smooth muscle.
In vitro

Imperatorin has estrogen-like activities in MCF-7 cells and stimulatory effects on ALP activity of Saos-2 osteoblast cells[1].

In vivo Imperatorin acutely administered at the doses of 10 and 20 mg/kg impairs anxiogenic effect of nicotine in the elevated plus maze (EPM) test. Imperatorin acts as irreversible inactivator of GABA-transaminase and increases the GABA content in the brain. Co-administration of subthreshold doses of imperatorin and nicotine produces an improvement inmemory processes in animals[2].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: MCF-7 cells
  • Concentrations: 0.1 μmol/L
  • Incubation Time: 24 h
  • Method:

    The MCF-7 cells are seeded into six-well plates in standard growth medium at a density of 100 000 cells per well and allowed to attach overnight (24 h). The growth medium is replaced with phenol red free DMEM containing 10% charcoal-dextran stripped human serum (hormone-free) for 72 h, after which the medium is refreshed and chemicals are added. After a further 24-h exposure, the cells are harvested and washed with PBS. Cell pellets, which are obtained by centrifugation at 1000 rpm for 15 minutes, are stained with 1 mL of freshly propidium iodide (PI) staining solution (0.1% of Triton X-100, 0.1% of sodium citrate, 50 μg/mL of PI and 10 μg/mL of RNase A in PBS). Flow cytometric analysis of cell cycle distribution is performed.

  • (Only for Reference)
Animal Research:

[2]

  • Animal Models: male Swiss mice
  • Dosages: 1, 10 and 20 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 54 mg/mL
(199.79 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 270.28
Formula

C16H14O4

CAS No. 482-44-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=CCOC1=C2C(=CC3=C1OC=C3)C=CC(=O)O2)C

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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