Atropine sulfate monohydrate
For research use only.
Molecular Weight(MW): 694.83
Atropine sulfate monohydrate is a competitive antagonist for the muscarinic acetylcholine receptor, used to decrease the production of saliva and secretions of the airway prior to surgery.
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|Description||Atropine sulfate monohydrate is a competitive antagonist for the muscarinic acetylcholine receptor, used to decrease the production of saliva and secretions of the airway prior to surgery.|
Atropine increases the release of the neurotransmitter dopamine into the superfusate in vitro at 100-500 mM and into the vitreous in vivo at 250 mg. Atropine induces spreading depression (SD) in the in vitro preparation. Atropine reduces the ERG b- and d-wave, leads to damped oscillations of RPE potentials, and reverses the ERG c-wave. Atropine suppresses myopia only at doses at which severe nonspecific side effects are observed in the retina. 
|In vivo||Atropine (1.0 mg/kg, i.p.), but not methylatropine (1.0 mg/kg, i.p.), prevents the enhancement of retention induced by both doses of the anticholinesterase when given immediately after training in mice.  Atropine administration effectively prevents bradycardia and second-degree heart block but induces pulsus alternans and hypertension in dogs.  Atropine has no effect on handling-induced acetylcholine output in the presence of 10 nM neostigmine, but causes greater and longer increases in the presence of 100 nM and 1000 nM neostigmine in rats.  Atropine not only blocks the rapid eye movement (REM) sleep increases induced by CGS but it also tends to decrease REM sleep compared to atropine preceding saline in rats.  Atropine decreases the time to exhaustion by 67% in intact rats and by 96.2% in adrenodemedullated (ADM) and also reduces the exercise-induced pituitary prolactin (PRL) release in both intact (50%) and ADM rats (90%). |
-  Schwahn HN, et al. Vis Neurosci,?000, 17(2), 165-176.
-  Boccia MM, et al. Neurosci Lett,?003, 345(2), 97-100.
-  Ko JC, et al. J Am Vet Med Assoc,?001, 218(1), 52-58.
|In vitro||DMSO||139 mg/mL (200.04 mM)|
|Water||139 mg/mL (200.04 mM)|
|Ethanol||139 mg/mL (200.04 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
|Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)|
|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
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Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04083768||Recruiting||Device: 15° and 30° wedge sponges||Elective Cesarean Section|Spinal Anesthesia||Xuzhou Medical University||December 30 2019||Not Applicable|
|NCT04045951||Recruiting||Other: Myopia prediction algorithm||Myopia||Sun Yat-sen University||August 3 2019||Not Applicable|
|NCT04044755||Recruiting||Other: Myopia prediction algorithm||Refractive Errors||Sun Yat-sen University||August 3 2019||Not Applicable|
|NCT04005014||Not yet recruiting||Other: Myopia prediction algorithm||Eye--Refractive Errors||Sun Yat-sen University||July 8 2019||Not Applicable|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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