(-)-Menthol

Catalog No.S4714 Synonyms: L-Menthol, Levomenthol, Menthomenthol, Menthacamphor

For research use only.

(-)-Menthol is a levo isomer of menthol and used as a cooling agent that strongly activates TRPM8.

(-)-Menthol Chemical Structure

CAS No. 2216-51-5

Selleck's (-)-Menthol has been cited by 1 Publication

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Biological Activity

Description (-)-Menthol is a levo isomer of menthol and used as a cooling agent that strongly activates TRPM8.
In vitro

(-)-Menthol induces cytotoxicity against murine leukemia WEHI-3 cells in vitro in a dosedependent manner. (-)-Menthol inhibits the growth of rat liver epithelial tumor cells and acts as a potent chemopreventive agent during DMBA initiation of rat mammary tumors. (-)-menthol inhibits N-acetyltransferase activity. It also is found to inhibit the DNA topoisomerase I, II alpha and beta and to promote NF-kappaB expression in human gastric cancer SNU-5 cells. Additionally, (-)-menthol induces human promyelocytic leukemia HL-60 cell death through the Ca2+ release from the endoplasmic reticulum[1].

In vivo The effects of (-)-menthol on WEHI-3 cells in vivo (BALB/c mice) were also examined, and it was observed that the Mac-3 and CD11b markers were decreased, indicating inhibition of differentiation of the precursor of macrophage and granulocyte. The weights of liver and spleen samples from mice treated with (-)-menthol were found to be decreased compared to untreated animals. (-)-Menthol has been shown to be toxic in animals, but in humans, it is considered to be safe, with a small cardio-accelerating effect. At high doses (-)-menthol may exert a depressant effect on the central nervous system in rodents. (-)-menthol statistically decreases the weights of the liver and spleen in the examined animals and also decreases the percentage of MAC-3 and CD11b cells in the blood.[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: Murine leukemia cell line (WEHI-3)
  • Concentrations: 0, 25, 50, 75 and 100 μM
  • Incubation Time: 24 h
  • Method:

    Approximately 2x105 cells (WEHI-3) are incubated in 12-well plates containing medium for 24 h without (control) or with various concentrations (0, 25, 50, 75 and 100 μM) of (-)-menthol. The cells are harvested and washed with PBS before PI staining. The percentage of viable WEHI-3 cells is determined by trypan blue exclusion and flow cytometry.

Animal Research:

[1]

  • Animal Models: Male BALB/c mice
  • Dosages: 1 mg/kg or 10 mg/kg
  • Administration: Oral administration

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 156.27
Formula

C10H20O

CAS No. 2216-51-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1CCC(C(C1)O)C(C)C

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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