SKF96365

For research use only.

Catalog No.S7999

2 publications

SKF96365 Chemical Structure

Molecular Weight(MW): 402.91

SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.

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Biological Activity

Description SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.
Targets
TRPC [1]
()
In vitro

SKF-96365 exhibits protective activity against MPP+ injury in PC12 cells and significantly inhibits apoptotic cell death in PC12 cells after MPP+ administration. SKF-96365 does not exert effects on necrotic cell death induced by MPP+ insult in PC12 cells. Because of its non-selective activity, SKF-96365 has been demonstrated to have effects on multiple other Ca2+ channels: it not only blocks high-voltage-activated (HVA) Ca2+ channels at typically utilized test concentrations, but also potently inhibits low-voltage -activated (LVA) T-type Ca2+ channels in HEK293 cells. The exact effect of SKF-96365 on intracellular calcium homeostasis might dependent on cell types and experimental models used[2].

In vivo SKF-96365 treatment inhibited the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade in vitro and in vivo[4].

Protocol

Cell Research:

[2]

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  • Cell lines: PC12 cells
  • Concentrations: 1 µM, 10 µM or 50 µM 
  • Incubation Time: 30 min
  • Method:

    To investigate whether SKF-96365 could protect PC12 cells from injury induced by MPP+ insult, cultured PC12 cells are pretreated with SKF-96365 in different concentrations (1 µM, 10 µM or 50 µM) 30 min before MPP+ addition. The cells viability is measured 24 h after MPP+ insult by using the cell proliferation reagent WST-1.


    (Only for Reference)
Animal Research:

[3]

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  • Animal Models: C57BL/6N mice
  • Dosages: 0.02 μmol & 0.2 μmol/2 μl/mouse
  • Administration: intrastriatally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 80 mg/mL (198.55 mM)
Water 80 mg/mL (198.55 mM)
Ethanol 80 mg/mL (198.55 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 402.91
Formula

C22H26N2O3.HCl

CAS No. 130495-35-1
Storage powder
in solvent
Synonyms N/A
Smiles Cl.COC1=CC=C(CCCOC(C[N]2C=CN=C2)C3=CC=C(OC)C=C3)C=C1

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID