Doxycycline Hyclate

For research use only.

Catalog No.S4163

10 publications

Doxycycline Hyclate Chemical Structure

Molecular Weight(MW): 512.94

Doxycycline is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).

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Biological Activity

Description Doxycycline is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).
Targets
MMP [6]
In vitro

Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts. [1]

Assay
Methods Test Index PMID
Western blot
AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1; 

PubMed: 26142707     


Doxycycline treatment decreases the levels of HSP90 client proteins in DLBCL cells. The DLBCL cells were treated with the indicated concentrations of doxycycline for 24 hrs. The levels of HSP90 client proteins in these cells were analyzed by western blotting.

HSP70 / HSP90 ; 

PubMed: 26142707     


Reduced protein levels of HSP70 and HSP90 in DLBCL cells treated with doxycycline. DLBCL cells were treated with doxycycline for 24 hrs, and the levels of HSP70 and HSP90 were analyzed by western blotting. 

pATM / ATM ; 

PubMed: 23282955     


Levels of phosphorylated ATM and overall ATM was determined in the indicated cell lines at the indicated times after treatment with 1μg/ml doxycycline, as detected by Western Blotting.

26142707 23282955
Immunofluorescence
E-cadherin / Vimentin ; 

PubMed: 29285218     


Doxycycline increases E-cadherin expression and decreases Vimentin expression, whereas thrombin partly counteracts this inhibitory effect in MCF-7 cells.

29285218
In vivo Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. [2] Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug's ability to inhibit excessive collagenase activity. [3] Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. [4] Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function. [5]

Protocol

Solubility (25°C)

In vitro DMSO 100 mg/mL (194.95 mM)
Water 100 mg/mL (194.95 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 512.94
Formula

C22H24N2O8.1/2C2H6O.HCl.1/2H2O

CAS No. 24390-14-5
Storage powder
in solvent
Synonyms N/A
Smiles O.Cl.Cl.CCO.CC1C2C(O)C3C(N(C)C)C(=C(C(N)=O)C(=O)C3(O)C(=C2C(=O)C4=C(O)C=CC=C14)O)O.CC5C6C(O)C7C(N(C)C)C(=C(C(N)=O)C(=O)C7(O)C(=C6C(=O)C8=C(O)C=CC=C58)O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04269434 Not yet recruiting Other: No Screening Neisseria Gonorrhoeae Infection|Chlamydia Trachomatis Infection Institute of Tropical Medicine Belgium May 2020 Not Applicable
NCT03709459 Recruiting Drug: Doxycycline STIs Prevention Kirby Institute|South Australian Health and Medical Research Institute|Monash University December 17 2019 --
NCT03558984 Not yet recruiting Drug: D-PLEX|Other: Standard of Care Surgical Site Infection|Sternal Infection|Cardiac Surgery PolyPid Ltd. October 2019 Phase 3
NCT02864550 Not yet recruiting Drug: Doxycycline|Other: Placebo Syphilis|Sexually Transmitted Infections British Columbia Centre for Disease Control August 15 2019 Phase 4
NCT03633123 Active not recruiting Drug: D_PLEX|Other: Standard of Care (SoC) Abdominal Surgery|Colon Surgery|Post-Op Infection PolyPid Ltd. October 4 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID