Doxycycline Hyclate (WC2031)

Catalog No.S4163 Synonyms: Doxycycline hydrochloride hemiethanolate hemihydrate

For research use only.

Doxycycline Hyclate (WC2031, Doxycycline hydrochloride hemiethanolate hemihydrate) is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).

Doxycycline Hyclate (WC2031) Chemical Structure

CAS No. 24390-14-5

Selleck's Doxycycline Hyclate (WC2031) has been cited by 29 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Doxycycline Hyclate (WC2031, Doxycycline hydrochloride hemiethanolate hemihydrate) is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).
Targets
MMP [6]
In vitro

Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SW1353 MXnGeY5kfGmxbjDhd5NigQ>? NFv6eVA2OCC3TR?= M{LmPWlvcGmkaYTpc44hd2ZiSVytNU1j\XSjLXnu[JVk\WRiTV3QNVMheHKxZIXjeIlwdiCrbjDoeY1idiCVV{GzOVMh[2WubIOgZZQhPTBidV2= MlTGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NkeyNlcoRjF5Mk[3NlI4RC:jPh?=
K-12 BW25113 NWn6RYxZSmGldHXybYNq\GGuIHHzd4F6 Mn3VNlQhcHK| NEnZRY9D[WO2ZYLpZ4ll[WxiYXP0bZZqfHliYXfhbY5{fCC7YX\RJIdmdmVvZHXmbYNq\W62IFXzZ4hmemmlaHnhJINwdGliSz2xNkBDXzJ3MUGzJIJqd2[rbH2gZZN{\XO|ZXSgZZMhdG:pIILl[JVkfGmxbjDv[kB3cWGkbHWgZ4VtdHNiYX\0[ZIhOjRiaILz NWnDUGRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzNFc{PzVpPkG5N|A4Ozd3PD;hQi=>
K-12 BW25113 NYL3PWxCSmGldHXybYNq\GGuIHHzd4F6 NUjCTWN[OjRiaILz NUTDUmRMSmGldHXybYNq\GGuIHHjeIl3cXS7IHHnZYlve3RiRYPjbIVzcWOqaXGgZ49tcSCNLUGyJGJYOjVzMUOgZolw\mmubTDoZZJjd3KrbnegdGNCOjSQIID0ZYM7QnmjZmGgdIxie22rZDDhd5Nme3OnZDDhd{Btd2dicnXkeYN1cW:wIH;mJJZq[WKuZTDj[YxteyCjZoTldkAzPCCqcoOgdJJmfHKnYYTl[EB4cXSqIEWgeW0hd2ZiSWDUS{Bnd3JiNDDodpM> M1HYT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{C3N|c2Lz5zOUOwO|M4PTxxYU6=
K562 NH\OPWxEgXSxdH;4bYNqfHliYYPzZZk> Ml20O|IhcHK| MlrNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomg[oxwfyCleYTvcYV1enluIFnDOVAhRSBzNTFOwG0v NHv5XWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4NlQ4Pid-MUm0PFI1PzZ:L3G+
HepG2 NWTudHpHS3m2b4TvfIlkcXS7IHHzd4F6 Mon2O|IhcHK| NFO1fYVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOjBizszNMi=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6MkS3Okc,OTl2OEK0O|Y9N2F-
THP1 Mk\JSpVv[3Srb36gZZN{[Xl? NEHiZlFU\WynY4Tpeol1gSCrbnTlfEwhemG2aX:gc4YhUUN3MDDmc5IhcHWvYX6gWGhROSClZXzsd{B1dyCLQ{WwJIZweiCSbHHzcY9lcXWvIH\hcINqeGG{dX2sJGlEPTBiPTCzMlEh|ryPLh?= NH7yboY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe0PFc5OSd-MUm3OFg4QDF:L3G+
THP1 NIPxVZpEgXSxdH;4bYNqfHliYYPzZZk> NIjxc|M4OiCqcoO= NIGyOlJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVUFBzIHPlcIx{KGGodHXyJFczKGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJ6NCCLQ{WwJF0hOjBizszNMi=> M{fWPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{S4O|gyLz5zOUe0PFc5OTxxYU6=
K562 NYG3[5FkS3m2b4TvfIlkcXS7IHHzd4F6 NEPWVZA4OiCqcoO= MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDmcI94KGO7dH;t[ZRzgSxiSVO1NEA:KDF3IN88UU4> NXfHUplkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5NlYyPzNpPkG5PVI3OTd|PD;hQi=>
HepG2 NGrNZ4NEgXSxdH;4bYNqfHliYYPzZZk> NF\mOJY4OiCqcoO= M3T5ZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzOCEQvF2u M4O3TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUK2NVc{Lz5zOUmyOlE4OzxxYU6=
HepG2 M3HQWmN6fG:2b4jpZ4l1gSCjc4PhfS=> NUD5WJZbPzJiaILz NUPlZo9YS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDJyIN88UU4> M4C2d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{SxNVMyLz5{MUe0NVE{OTxxYU6=
Neuro2a NGrRc3NHfW6ldHnvckBie3OjeR?= MXOxJJVO M37IZmRm[3KnYYPlJIlvKDdvRFjDJIxmfmWuczDpckBFcGO{Nz3k[YZq[2mnboSgcY92e2ViTnX1do8z[SClZXzsd{BifCBzIIXNJIJ6KEyFLV3TM2dENU2VIHHuZYx6e2m| NIP2PHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke4PVY2Pyd-Mk[3PFk3PTd:L3G+
HepG2 NWfVd4lPS3m2b4TvfIlkcXS7IHHzd4F6 M2q4XVczKGi{cx?= MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bu\WG|dYLl[EBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBESzVyIE2gNlAh|ryPLh?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF3NUS2N{c,OjdzNUW0OlM9N2F-
HepG2 NGDDeIxEgXSxdH;4bYNqfHliYYPzZZk> M{O1TlczKGi{cx?= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiQ1O1NEA:KDJyIN88UU4> NUn0N2lmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVQ{QTVpPkK3OlU1Ozl3PD;hQi=>
CD NFvlW5hCdnSrYnHjeIVzcWGuIHHzd4F6 MnzrNlUhfWdxbXy= M2XScFczKGi{cx?= NGjle|RCdnSrYnHjeIVzcWGuIHHjeIl3cXS7IHHnZYlve3RiUnnjb4V1fHOrYTDwdo94[Xqna3npJJN1ei5iQoLlbY5tKGmwZnXjeIVlKGmwIFPEJJJifCCycnntZZJ6KHC3bH3vcoFzgSC4YYPjeYxieiCnbnTveIhmdGmjbDDj[YxteyCjc4Pld5Nm\CCjczDiZYN1\XKrYXygd4hieGViY3jhcodmKGG2IEK1JJVoN22uIH3lZZN2emWmIEeyJIhzeyCyb4P0JIlv\mWldHnvckBjgSCKb3XjbJN1KDN|MkW4M5Bp[Wyub3nkbY4he3SjaX7pcoch[mG|ZXSg[ox2d3J? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OUO1NEc,OjhyOEmzOVA9N2F-
A673 NX\3dJBxeUiWUzDhd5NigQ>? MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NH\HN2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 NVPXUWx[eUiWUzDhd5NigQ>? M1rrUJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= Mo[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
skeletal myoblast cells M4rX[GN6fG:2b4jpZ4l1gSCjc4PhfS=> Mnn6SG5FUTpiQ4n0c5RwgGmlaYT5JIlvKF[rdILvMEA4OiCqb4XyMEBqdiC{YYSgd4tmdGW2YXygcZlw[myjc4SgZ4VtdHNuIFnDOVAhRSBzND60PUDPxE1w MmDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVQ{Oy9pPlPoSW1DVDxxYU6=
Assay
Methods Test Index PMID
Western blot AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1 ; HSP70 / HSP90 ; pATM / ATM 26142707 23282955
Immunofluorescence E-cadherin / Vimentin 29285218
In vivo Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. [2] Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug's ability to inhibit excessive collagenase activity. [3] Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. [4] Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 512.94
Formula

C22H24N2O8.1/2C2H6O.HCl.1/2H2O

CAS No. 24390-14-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCO.CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.O.Cl.Cl

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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