Chlortetracycline HCl (NSC 13252)

For research use only.

Catalog No.S4156 Synonyms: 7-Chlorotetracycline HCl, Aureomycin monohydrochloride

Chlortetracycline HCl (NSC 13252) Chemical Structure

CAS No. 64-72-2

Chlortetracycline HCl (NSC 13252, 7-Chlorotetracycline, Aureomycin monohydrochloride) is the first identified tetracycline antibiotic.

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Biological Activity

Description Chlortetracycline HCl (NSC 13252, 7-Chlorotetracycline, Aureomycin monohydrochloride) is the first identified tetracycline antibiotic.
In vitro

Chlortetracycline HCl obtained from Streptomyces aureofaciens, is a broad-spectrum antibacterial agent with activity against a wide range of Gram-positive and Gram-negative bacteria, including obligate anaerobes. Chlortetracycline HCl binds strongly to a single site on the 30S subunit. Chlortetracycline HCl inhibits bacterial growth primarily by inhibiting protein synthesis at the level of the ribosome. [1] Chlortetracycline HCl is activity against Streptococcus pyogelles, Diplococcus pneuliloniap, Bacillus subtilis, Streptococcus faecalis, Bacillus subtilis, Mycobacterium ranae with MIC of 0.292, 0.098, 0.195, 0.3, 0.15, 0.3 mg/L. [2]


Solubility (25°C)

In vitro DMSO 5 mg/mL warmed (9.7 mM)
Water 1 mg/mL (1.94 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 515.34


CAS No. 64-72-2
Storage powder
in solvent
Synonyms 7-Chlorotetracycline HCl, Aureomycin monohydrochloride
Smiles CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C(C=CC(=C41)Cl)O)O)O)O)C(=O)N)N(C)C)O.Cl

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID