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Chlortetracycline HCl (NSC 13252) Antineoplastic and Immunosuppressive Antibiotics inhibitor

Name: Chlortetracycline HCl (NSC 13252)
Brand: Selleck Chemicals
SKU: S4156
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4156

Chlortetracycline HCl (NSC 13252, 7-Chlorotetracycline, Aureomycin monohydrochloride) was the first identified tetracycline antibiotic.

Chemical Structure

Molecular Weight: 515.34

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Quality Control

Batch: Purity: 99.52%

99.52

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Other Antineoplastic and Immunosuppressive Antibiotics Inhibitors	Staurosporine (STS) Cyclosporin A Puromycin Dihydrochloride Oligomycin A (MCH 32) Geldanamycin Honokiol Streptozotocin (STZ) Cephalomannine Sodium Monensin (NSC 343257) 10-Deacetylbaccatin-III

Solubility

In vitro
Batch:

DMSO : 10 mg/mL (19.4 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 2 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	515.34	Formula	C₂₂H₂₃ClN₂O₈.HCl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	64-72-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	7-Chlorotetracycline HCl, Aureomycin monohydrochloride			Smiles	CC1(C2CC3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C(C=CC(=C41)Cl)O)O)O)O)C(=O)N)N(C)C)O.Cl

Mechanism of Action

In vitro

Chlortetracycline HCl (NSC 13252), obtained from Streptomyces aureofaciens, is a broad-spectrum antibacterial agent with activity against a wide range of Gram-positive and Gram-negative bacteria, including obligate anaerobes. It binds strongly to a single site on the 30S subunit and inhibits bacterial growth primarily by inhibiting protein synthesis at the level of the ribosome. This compound is active against Streptococcus pyogelles, Diplococcus pneuliloniap, Bacillus subtilis, Streptococcus faecalis, Bacillus subtilis, Mycobacterium ranae with MIC of 0.292, 0.098, 0.195, 0.3, 0.15, 0.3 mg/L.

References

[1] https://pubmed.ncbi.nlm.nih.gov/1592696/
[2] https://pubmed.ncbi.nlm.nih.gov/4580101/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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