BAY 11-7082

Catalog No.S2913 Synonyms: BAY 11-7821

BAY 11-7082 Chemical Structure

Molecular Weight(MW): 207.25

BAY 11-7082 is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. Also inhibiting components of the ubiquitin system.

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Cited by 84 Publications

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Choose Selective IκB/IKK Inhibitors

Biological Activity

Description BAY 11-7082 is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. Also inhibiting components of the ubiquitin system.
Targets
E2-conjugating enzymes [6]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
10 μM
In vitro

BAY 11-7082 completely and specifically abrogates NF-κB DNA binding, downregulating the NF-κB-inducible cytokine IL-6 and inducing apoptosis. [1] BAY 11-7082 (< 8 μM) is able to effectively inhibit both basal and TNFα stimulated NFκB luciferase activity in a dose dependent manner. BAY 11-7082 (8 μM) strongly inhibits the rate of proliferation in NCI-H1703 cells. [2] Bay 11-7082 (5 μM) rapidly and efficiently reduces the DNA binding of NF-kappaB in HTLV-I-infected T-cell lines and down-regulates the expression of the antiapoptotic gene, Bcl-x(L), whereas it has little effect on the DNA binding of another transcription factor, AP-1. Bay 11-7082-induced apoptosis of primary ATL cells is more prominent than that of normal peripheral blood mononuclear cells, and apoptosis of these cells is also associated with down-regulation of NF-kappaB activity. Bay 11-7082 (5 μM) selectively induces apoptosis of HTLV-I–infected T-cell lines associated with down-regulation of the expression of cyclin D1, cyclin D2, and Bcl-xL. [3] BAY 11-7082 (100 μM) prevents the nuclear translocation of p65 elicited by NMDA and the NMDA-induced increase of NF-κB binding in mouse hippocampal slices. BAY 11-7082 prevents NMDA toxicity occurring in CA1 region of hippocampal slices with 40% neuroprotection at 20 μM and 70% neuroprotection at 100 μM. [4] BAY 11-7082 at all concentrations tested significantly inhibits NF-κB p65 DNA-binding activity in adipose tissue, whereas in skeletal muscle, BAY 11-7082 at 50 μM and 100 μM significantly inhibits NF-κB p65 DNA-binding activity. BAY 11-7082 (100 μM) reduces IKK-β protein in human adipose tissue and skeletal muscle. BAY 11-7082 (100 μM) significantly decreases the release of TNF-α from adipose tissue, whereas the release of IL-6 and IL-8 is significantly inhibited at all concentrations of BAY 11-7082 tested. BAY 11-7082 (50 μM) significantly decreases the release of TNF-α, IL-6, and IL-8 in skeletal muscle. [5] BAY 11-7082 is also found to inactivate the E2-conjugating enzymes Ubc (ubiquitin conjugating) 13 and UbcH7 and the E3 ligase LUBAC (linear ubiquitin assembly complex), and thus induces B-cell lymphoma and leukaemic T-cell death. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa MnLvSpVv[3Srb36gRZN{[Xl? MUmxNOKh|ryP MWexMlUhcA>? NEXUbmNi[m:uaYPo[ZMhSlCDIHnu[JVk\WRidYCgdoVofWyjdHnvckBw\iCITjDhcoQhVU2SLUpCpC=> MlXuNlU4QTd2M{e=
SiHa  NGH2U29HfW6ldHnvckBCe3OjeR?= M1;1VlExyqEQvF2= NVLtO5V[OS53IHi= MULhZo9tcXOqZYOgRnBCKGmwZIXj[YQhfXBicnXneYxifGmxbjDv[kBHViCjbnSgUW1RNToEoB?= NVX4[otnOjV5OUe0N|c>
ARPE-19 MVrGeY5kfGmxbjDBd5NigQ>? MnP0NUDPxE1? MonjNE42KGh? MlPYd5VxeHKnc4Pld{BVTy2rbnT1Z4VlyqCLTD24xsBxem:vb4TldkBi[3SrdnH0bY9v NXq5UYs5OjV3OUOwNlk>
HCT116 MXHGeY5kfGmxbjDBd5NigQ>? Mo\6OUDPxE1? MkLuNkBp MVjEUXNQ MnPVZZR1\W63YYTld{B{cWy7bXHybY4ucW6mdXPl[EBld3ewcnXneYxifGmxbjDv[kBkgWOuaX6gSFE> MV6yOVQ4QTd{Mx?=
HMECs NX\RPVQ4TnWwY4Tpc44hSXO|YYm= NXH1NXY4PcLizszN MWeyJIg> NID6SIZi[m:uaYPo[ZMhXE6ILd8xMYlv\HWlZXSgWmNCVS1zIHX4dJJme3Orb39CpC=> NGPjPZYzPTF7M{GxOi=>
A549  NITqV4NHfW6ldHnvckBCe3OjeR?= NHHObWUyOOLCidM1US=> MnPCNVIhcA>? MnXjd5VxeHKnc4Pld{BFfmxvMzDpcoR2[2WmIHHjeIl3[XSrb36gc4YheDZ3 NEDIbIszPTF3NkiwNC=>
RAW 264.7 M2K1R2Z2dmO2aX;uJGF{e2G7 M1zWfFXDqM7:TR?= MlLkNUBp MmWzbY5pcWKrdIOgWG5HNc7zIHHu[EBKVC1zMjDwOFAheHKxZIXjeIlwdg>? NF:yVVMzPTBzOUW2Oy=>
macrophages MlfPSpVv[3Srb36gRZN{[Xl? M{P6XFUhyrWP M1XPZlMhcA>? NFuyT41x[XK2aXHscJkh[myxY3vzJHlRTlNvaX7keYNm\CCneIDy[ZN{cW:wIH;mJIlPV1NiYX7kJGNQYC1{wrC= NHLJZ2UzPDl4N{i5PC=>
HUVECs NFy4VHpHfW6ldHnvckBCe3OjeR?= M2m5SVMuOzBizszN NVHqWFdWOSCq NHyyZ2Fz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mJI1qWi1zNE\hJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NVTtO5BGOjR6NkO5OlU>
HeLa  M1HZVGZ2dmO2aX;uJGF{e2G7 NFvqU3E2yqEQvF2= NFjaOHYzPCCq NFzvW3JFVVOR MV;y[YR2[2W|IITo[UBi[3Srdnn0fUBw\iCWTl[t{tEheHKxbX;0[ZLDqA>? NV7FNVJmOjR4NUe3PFM>
A549 NVTMSGR2TnWwY4Tpc44hSXO|YYm= MlP6NVAh|ryP NGDNW4oy6oDLaB?= M{P4VolvcGmkaYTzJJRp\SCrbnPy[YF{\SCxZjDwbI9{eGixLVpOvmLPuSCrbjDQRVExOy2rbn\lZ5Rm\CCldXz0eZJme8Li MlnRNlQ3OTJ2OEi=
HUVEC M2Pqb2Z2dmO2aX;uJGF{e2G7 NVjYdHpkOjBiwsXN NWfXT49LOC53IHi= Ml34SG1UVw>? M1vTbJBz\X[nboTzJJRp\SCrbnT1Z5Rqd25ib3[gSWFOKGW6cILld5Nqd25? MonlNlQ2PTF{MEm=
A549RT-eto M{fDfmFxd3C2b4Ppd{BCe3OjeR?= Mmr6NVDDqM7:TR?= M3\SW|I1KGh? MXTEUXNQ Mk\BZYNk\WyncnH0[ZMhTkWUTz3t[YRq[XSnZDDhdI9xfG:|aYO= M{L4elI1PTN3MEiz
THP-1  M4m3bWZ2dmO2aX;uJGF{e2G7 MWWwMlEwOcLizszN MWKwMlUhcA>? MljkZYJzd2ejdHXzJHRPTi4QsTDz[YNz\XSrb36gZZMhf2WubDDhd{B1cGViaX7jdoVie2WmIIPlZ5JmfGmxbjDv[kBKVC14IHHu[EBKVC1zzsK= NYTwR5JoOjR|N{i1N|Y>
SKCXCR2  MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYOyJOK2VQ>? M3fhWFQ5KGh? MmC0[IVkemWjc3XzJINmdGxicILvcIln\XKjdHnvckB{cWewaX\pZ4FvfGy7 M3TXW|I1Ozd4N{S3
SKCXCR2  MUnGeY5kfGmxbjDBd5NigQ>? NGPudIozKML3TR?= Mo\aOFghcA>? M4qwfYJtd2OtczD0bIUhS1iFTEGtbY5lfWOnZDDj[YxtKGmwdnHzbY9v Mor1NlQ{PzZ5NEe=
OVCXCR2 M1vmPGZ2dmO2aX;uJGF{e2G7 MmDoNkDDvU1? NYrUbplpPDhiaB?= NFi4bXhjdG:la4OgeIhmKEO[Q1yxMYlv\HWlZXSgZ4VtdCCrbo\hd4lwdg>? MnXFNlQ{PzZ5NEe=
DSCs  Mn3xSpVv[3Srb36gRZN{[Xl? MYKyMlUh|ryP MmHaNE42KGh? Mn7rdoV3\XK|ZYOgeIhmKGWwaHHuZ4Vu\W62IH;mJGNEVDJxQ1PSNkBmgHC{ZYPzbY9vKG:oIFTTR5MhcW6mdXPl[EBjgSCLTD2zNy=> M13TS|I1OzR2MkSw
WPs MYPGeY5kfGmxbjDBd5NigQ>? M3vVNFI2KM7:TR?= NFz0T4o2KG2rbh?= MUDzeZBxemW|c3XzJGFVWCCjbnSgendHKHOnY4LleIlwdg>? MYGyOFM{OTJyNx?=
A549RT-eto M1jzSmFxd3C2b4Ppd{BCe3OjeR?= NUTt[FlXOTEEoN88US=> NFrrcWgzPCCq NVnLVlc{[WOlZXzldoF1\XNiRkG0JIV5fHKjY4StcYVlcWG2ZXSgZZBweHSxc3nzJINwdWKrbnXkJJRz\WG2bXXueEB4cXSqIF[xOC=> MmDQNlQzOjB5MkW=
A549RT-eto NGjWbGhHfW6ldHnvckBCe3OjeR?= NEnkSlIyOMLizszN MUmyOEBp NWPzNFlm\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJIxmfmWuczDv[kBPTi4QulKgZY5lKFBvZ4FCpC=> M1mzNlI1OjJyN{K1
FaDu NFTFdm5HfW6ldHnvckBCe3OjeR?= MXKyJIg> MVzpcohq[mm2czDwOlUh\XiycnXzd4lwdiCjbnSgZoxw[2u|IGTOSu6yNWmwZIXj[YQhXFeLU2Sg[ZhxemW|c3nvci=> NYLBS3UyOjR{MkC2NlI>
IVD  NWPlUoRDTnWwY4Tpc44hSXO|YYm= NEDQfpEyOMLizszN M2Sy[VMh\A>? MUjy[ZZmenOnczDUUmYu|rIkgKPt[YRq[XSnZDDzeZBxemW|c3nvckBw\iC2aHWg[Il{[yCvYYTybZghdWGlcn;tc4xm[3WuZYOgZYdoemWlYX6gZY5lKGOxbHzh[4VvKEmL M1Lp[|I1OTd4OEC4
IVD  MoPGSpVv[3Srb36gRZN{[Xl? NFLtSI0yOMLizszN NIrQRW0{KGR? M2PFTIFjem:pYYTld{BVVkZvzsJihLNqdmS3Y3XkJJVxNXKnZ4XsZZRqd25ib3[gRWRCVVSVLUSgZY5lKEGGQV3UV{02yqB? MoTSNlQyPzZ6MEi=
iNKT M2G4ZWZ2dmO2aX;uJGF{e2G7 MV[xNE8yODBizszN MmPINE42KGh? MmLlbY5pcWKrdIOgeIhmKGmwZIXjeIlwdiCxZjDBNmFTKG2UTlGgZY5lKG:2aHXyJIZi[3Sxch?= MkLBNlQyOjR2NUO=
PC-3 NX3uTplsTnWwY4Tpc44hSXO|YYm= NHXlOmozNjVxNT:xNEDPxE1? NX6zUJdjOC53IHi= NIn5d2FjdG:la4OgTWdHNUmLLXnu[JVk\WRiU2TTJI1TVkFiZYjwdoV{e2mxbtMg NV3JXYEyOjRyNUW1NlA>
THP-1  NHjy[3ZHfW6ldHnvckBCe3OjeR?= MUWxNOKh|ryP MkjpNeKhcA>? NX;rZ4dT[WKxbHnzbIV{KHSqZTDl[oZm[3Rib3[gdmhUWDJ5IH;uJHNTNUFibWLORS=> NGfGeIEzOzl|OUO5PC=>
A549 NE\lem1HfW6ldHnvckBCe3OjeR?= M37MR|HDqM7:TR?= NVn6c|ZSPDhiaB?= NUDoeIpv\W6qYX7j[ZMhfGinIIXwMZJm\3WuYYTpc44hd2ZiSd86RkBidmRic4Xid4VyfWWwdDDk[YNz\WG|ZTDpckBD[XhiZYjwdoV{e2mxbjDpcoR2[2WmIHL5JINwdWKrbnXkJJN1cW23bHH0bY9v MX[yN|kxODB6MB?=
A549 NX;PeXE2SXCxcITvd4l{KEG|c3H5 MXyxxsDPxE1? MnWxOFghcA>? NWT2PG1EemWmdXPld{B1cGViY3XscEBl\WG2aDDpcoR2[2WmIHL5JINwdWKrbnXkJJN1cW23bHH0bY9v MoKyNlM6ODByOEC=
NCI-N87 MmTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mor5NVAwOjBxM{Cg{txO MoGzOk8zPCCq NWD5TYdJe3WycILld5NmeyClZXzsJJZq[WKrbHn0fUB{cWewaX\pZ4FvfGy7 MXSyN|g1PjV2NR?=
AGS NFG4PG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVuxNE8zOC9|MDFOwG0> MVS2M|I1KGh? MmCxd5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDzbYdvcW[rY3HueIx6 M4DCSlI{QDR4NUS1
MGC80-3 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3G5RVExNzJyL{OwJO69VQ>? NYHLZWNoPi9{NDDo Mofhd5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDzbYdvcW[rY3HueIx6 MV2yN|g1PjV2NR?=
HGC-27  NYP6N2NPTnWwY4Tpc44hSXO|YYm= NFjEUok4NjVxMUWvN|Ah|ryP M4C2XVYhcA>? MmjxbY5lfWOnczD0bIUh\GWyaH;zdIhwenmuYYTpc44h[W6mIIXwMZJm\3WuYYTpc44hd2ZiSd86Ru6y MXqyN|g1PjV2NR?=
MGC80-3 M2DpUWZ2dmO2aX;uJGF{e2G7 NIDIVYk4NjVxMUWvN|Ah|ryP NHruXmc3KGh? M13mTYlv\HWlZYOgeIhmKGSncHjvd5Bpd3K7bHH0bY9vKGGwZDD1dE1z\We3bHH0bY9vKG:oIFpOvmLPuQ>? MXGyN|g1PjV2NR?=
HGC-27  M2OzNGFxd3C2b4Ppd{BCe3OjeR?= M1XnelcvPS9zNT:zNEDPxE1? NETLcJE3KGh? NEfZe5BqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIITpcYUuKGGwZDDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MlLKNlM5PDZ3NEW=
HBE  MUDGeY5kfGmxbjDBd5NigQ>? MUOxNO69VQ>? NIH3N|c{cA>? M{izZoFjd2yrc3jld{B1cGViaX7jdoVie2W|IH;mJGlNNTZiZYjwdoV{e2mxbjDpcoR2[2WmIHL5JGNUTQ>? NGX3Zo0zOzh{NEC4PS=>
HepG2 NVT2[3VXTnWwY4Tpc44hSXO|YYm= NUjj[3I2OC5|L{GvN{DPxE1? NHnBcmw1QCCq NV\VbZlRemWmdXPld{BKVDZvaX7keYNm\CCST16xJIV5eHKnc4Ppc44> M1PCOFI{PzlzOEOz
THP-1 NGTyVnJHfW6ldHnvckBCe3OjeR?= M2\5b|UhyrWP NYfyUnNOOSCq MoLwSG1UVw>? M4rsOYlvcGmkaYTzJG1VSi2rbnT1Z4VlKE6IzsrCJIFkfGm4YYTpc44> MlfaNlM3OzR{MUi=
THP-1  MlT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[1JOK2VQ>? M2HV[FQwQCCm MorDSG1UVw>? MlTVdoVlfWOnczD0bIUhfmmjYnnsbZR6KG:oIHnueJJi[2WubIXsZZLDqE2WQh?= NUXtOYpHOjN4M{SyNVg>
MDM MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHPZVJWPSEEtV2= Mni5OE85KGR? NGLQd|JFVVOR NYX1fJV2emWmdXPld{B1cGVidnnhZoltcXS7IH;mJIlvfHKjY3XscJVt[XMEoF3URi=> NWfi[o16OjN4M{SyNVg>
AM NWT6[GdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHGxZpU2KML3TR?= NILhZo01NzhiZB?= NInWe3JFVVOR MWjy[YR2[2W|IITo[UB3cWGkaXzpeJkhd2ZiaX70doFk\WyudXzhduKhVVSE M4q3VVI{PjN2MkG4
RAW 264  NXnHeYhETnWwY4Tpc44hSXO|YYm= MWCwMlIuPSEEtV2= MWWzNE83OC97MDDtbY4> NY[1V3h1cW6qaXLpeJMhfGinIIDoc5NxcGG2YYPlJIFkfGm4aYT5JI9nKFCWUEHCxsA> NUDjUmRpOjN3N{izNFI>
HUVEC MnLQSpVv[3Srb36gRZN{[Xl? Mlf6NVDDqM7:TR?= M3j6T|AvPSCq Ml3VSG1UVw>? NUDadGpC[2:3boTldoFkfGW|IITo[UBtd3O|IH;mJHRq\TJibWLORS=> NX;D[YJNOjN3NkO2N|I>
HT29 MkXhSpVv[3Srb36gRZN{[Xl? NHLOVnIyOC9|MD:xNFAh|ryP NUXHRoZ4OSCq MWrpcohq[mm2ZYOgZo91cCCWV1XBT{1qdmS3Y3XkJJAyODBicILvZ4V{e2mwZx?= NXXoRYpZOjN3MkexOVQ>
HT29 M{C3OWZ2dmO2aX;uJGF{e2G7 Mn\3NVAwOzBxMUCwJO69VQ>? MX2xJIg> NGS5W4ZqdmirYnn0d{BVVkZvaX7keYNm\CCyaH;zdIhwenmuYYTpc44h[W6mIHTl[5Ji\GG2aX;uJI9nKEoQulNOtS=> NGjvOFMzOzV{N{G1OC=>
MM.1S NUnB[XhDSXCxcITvd4l{KEG|c3H5 MortN|AhyrWP MWOzJIg> MkXnbY5lfWOnczDNUUBk\WyuIHTlZZRpKGmwdn;seoV{KG6nY4Lvd4l{ MV2yN|UzPzF3NB?=
KMS-12-BM NInrRW1CeG:ydH;zbZMhSXO|YYm= M1TBWVMxKML3TR?= MVizJIg> M2K4e4lv\HWlZYOgUW0h[2WubDDk[YF1cCCrbo\vcJZmeyCwZXPyc5Nqew>? M1vmTlI{PTJ5MUW0
BAFs NIK4XpFHfW6ldHnvckBCe3OjeR?= MVGwMlUwOcLizszN NXnufmNTOjRiaB?= MoXwbY5pcWKrdIOgWG5H|rFxRFXYJIlv\HWlZXSgR3lROTmDMdMgeJJidnOlcnnweJM> NFjYc2UzOzR6NUS1Oy=>
SP6.5 MoDkSpVv[3Srb36gRZN{[Xl? M{HFb|Uh|ryP NFXQeJUzKGh? M3HxNIRm[3KnYYPld:KhfHKjboPsc4NifGmxbjDv[kBxPjViaX6geIhmKG63Y3zleZPDqA>? NFL3NYwzOzR2M{C4Oi=>
VUP MnqzSpVv[3Srb36gRZN{[Xl? NIHNOFE2KM7:TR?= MnnkNkBp NXfaVWk3\GWlcnXhd4V{yqC2cnHud4xw[2G2aX;uJI9nKHB4NTDpckB1cGViboXjcIV2e8Li MV:yN|Q1OzB6Nh?=
OCM1 MnvaSpVv[3Srb36gRZN{[Xl? M3z0RVUh|ryP MkXyNkBp MoS4[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh NVfFZ41xOjN2NEOwPFY>
OM431 MY\GeY5kfGmxbjDBd5NigQ>? NV7m[lRmPSEQvF2= NVTWdZVTOiCq NHnEWm9l\WO{ZXHz[ZPDqHS{YX7zcI9k[XSrb36gc4YheDZ3IHnuJJRp\SCwdXPs[ZV{yqB? M3vxRlI{PDR|MEi2
SP6.5 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;CT48zNjVvMkCg{txO NELqSXYzPCCqwrC= M{naNGlEPTB;NTFOwG0tKGW6aHnibZR{KHO2cn;u[{BidnSrLYDyc4xq\mW{YYTpeoUh\W[oZXP0d{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NW\qN|c{OjN2NEOwPFY>
VUP NGHVO4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUSyMlUuOjBizszN NF3Rcm4zPCCqwrC= NYi3eG9rUUN3ME21JO69VSxiZYjobYJqfHNic4Tyc45oKGGwdHmtdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NWKyc3pFOjN2NEOwPFY>
OCM1 MojHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWCyMlUuOjBizszN Mn;VNlQhcMLi NVX2SlJOUUN3ME21JO69VSxiZYjobYJqfHNic4Tyc45oKGGwdHmtdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MlXjNlM1PDNyOE[=
OM431 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnLfldZOi53LUKwJO69VQ>? NV\6SlJROjRiaNMg Ml;STWM2OD13IN88UUwh\XiqaXLpeJMhe3S{b37nJIFvfGlvcILvcIln\XKjdHn2[UBm\m[nY4TzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NWrrUldsOjN2NEOwPFY>
SP6.5 MkXnRZBweHSxc3nzJGF{e2G7 NYHkNo9bPSEQvF2= M1y5cVI1KGkEoB?= MkDobY5lfWOnczDhdI9xfG:|aYRCpC=> M2\obVI{PDR|MEi2
VUP MmnDRZBweHSxc3nzJGF{e2G7 MlG2OUDPxE1? M3jCcFI1KGkEoB?= NXO3fFhkcW6mdXPld{BieG:ydH;zbZPDqA>? NVvPWZdkOjN2NEOwPFY>
OCM1 NW\zNYp7SXCxcITvd4l{KEG|c3H5 NGrafmI2KM7:TR?= MnG4NlQhcMLi MWfpcoR2[2W|IHHwc5B1d3Orc9Mg NEPLSIQzOzR2M{C4Oi=>
OM431 M3PGTWFxd3C2b4Ppd{BCe3OjeR?= NGPWT4Y2KM7:TR?= MWSyOEBpyqB? NE\H[3VqdmS3Y3XzJIFxd3C2b4Ppd:Kh NYj3dnV{OjN2NEOwPFY>
SP6.5 M{\JWWZ2dmO2aX;uJGF{e2G7 M1zneFUh|ryP M2jpUlEzKGh? NWjkT3dSemWmdXPld{B1cGVibXnndoF1cW:wwrC= NVrTfm06OjN2NEOwPFY>
VUP MkjMSpVv[3Srb36gRZN{[Xl? MXW1JO69VQ>? M2LmOlEzKGh? MoPHdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? MU[yN|Q1OzB6Nh?=
OCM1 MYHGeY5kfGmxbjDBd5NigQ>? MXq1JO69VQ>? M4rwVlEzKGh? NXj2VYhQemWmdXPld{B1cGVibXnndoF1cW:wwrC= M{DZflI{PDR|MEi2
OM431 NWP5R2E1TnWwY4Tpc44hSXO|YYm= M1H1S|Uh|ryP NFKxT2UyOiCq M1iwV5Jm\HWlZYOgeIhmKG2rZ4LheIlwdsLi NUfiT21WOjN2NEOwPFY>
HBL-1  MmWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnsb3U2O8LizszN MnrKNlQwPDhxN{KgbC=> NITxVphFVVOR Mn22d4xwf3NiY3XscEBoem:5dHigcY9l\XO2bIm= NGLvT|IzOzR2MUezNC=>
RAW 264.7 NHvrN2ZHfW6ldHnvckBCe3OjeR?= M1PlPVIuOTVizszN M4XPWFEhcA>? MVXEUXNQ MVnzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJGlMUyCoYX3pcJkhdWWvYnXydy=> MYWyN|Q1OTd|MB?=
IL-1R  MVLGeY5kfGmxbjDBd5NigQ>? NUTWZmtDOi1zNTFOwG0> NGLwbo0yKGh? M17xZ2ROW09? NV7xTnFUe3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCLS1ug[oFucWy7IH3lcYJmenN? MV[yN|Q1OTd|MB?=
RAW 264.7 MXTGeY5kfGmxbjDBd5NigQ>? MlewNVUh|ryP NF3pZmMyKGh? MkjLSG1UVw>? Mom2d5VxeHKnc4Pld{B1cGViYXP0bZZifGmxbjDv[kBidmRiSl7L M3y5T|I{PDRzN{Ow
IL-1R  NXPwRmUyTnWwY4Tpc44hSXO|YYm= Mm[0NVUh|ryP NIS0S5QyKGh? MlnnSG1UVw>? NH2xT|R{fXCycnXzd4V{KHSqZTDhZ5RqfmG2aX;uJI9nKGGwZDDKUms> M3;3Z|I{PDRzN{Ow
U2OS NUG2S3BTTnWwY4Tpc44hSXO|YYm= MVKxOUDPxE1? NXvzbIRvOSCq NEO2T|JFVVOR MYrwdoV3\W62czD0bIUhVFCVLTDvdkBKVC1zLYP0bY12dGG2ZXSg[o9zdWG2aX;uJI9nKEt4Mz3wWYIh[2ijaX7zxsA> M3G5WVI{PDRzN{Ow
MT‐1  MVHGeY5kfGmxbjDBd5NigQ>? NXXxTZh2QMLiwsXt NX3OXmtVOyCq NUTaZm84\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdQKBmFOWQWSzJIFv\CCy4pEQOGVDWDF? Mm\PNlMzPzh2N{m=
MT‐2  NITrT4lHfW6ldHnvckBCe3OjeR?= M4XR[FjDqML3bR?= M4S4VlMhcA>? NGTWcZRl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDw5qCRW1SDVEOgZY5lKHEkgKC0SWJROQ>? Ml;qNlMzPzh2N{m=
MT‐1  NHHxS5dHfW6ldHnvckBCe3OjeR?= NVewdZFRQMLiwsXt MVmzJIg> NUDwdY1m\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[geIhmKHB4NTDzeYJ2dmm2IH;mJG5H6oDSzsrCxsA> MmjRNlMzPzh2N{m=
MT‐2  NEf0XZVHfW6ldHnvckBCe3OjeR?= NULDXZRYQMLiwsXt NVjSR3M1OyCq MnHE[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZidHjlJJA3PSC|dXL1col1KG:oIF7G5qCR|rqEwrC= NYfzbJVJOjN{N{i0O|k>
MCF-7  MkG4SpVv[3Srb36gRZN{[Xl? NF;4N|IzNjVvMUWg{txO NFLOfWUxNjViaB?= NUDaV2tmTE2VTx?= NV7sSFhH[2G3c3XzJJRp\SCpcnHkeYFtKGyxc4Ogc4Yh[2WubDDh[Ihme2mxbh?= MojCNlMxQTN{Mke=
HaCaT  NXrYO2k4TnWwY4Tpc44hSXO|YYm= MljQOU4xyqEQvF2= M1rkO|HDqGkEoB?= NYS3fWVX[XS2ZX71ZZRmeyC2aHWgWGNQUC2rbnT1Z4VlKHC{b3T1Z5Rqd25ib3[gTWwuPsLi NWjY[JVYOjNyNEGxOlg>
A549  NYS4WJloTnWwY4Tpc44hSXO|YYm= MV[xJIg> NVzac4RscW6qaXLpeJMhVFSDLXnu[JVk\WRiU2CtRUBuWk6DIIDyc4R2[3Srb39CpJNq\26rZnnjZY51dHl? NWrWVYVVOjNyM{GyNVM>
OA chondrocytes  MX\GeY5kfGmxbjDBd5NigQ>? MYGxNEDPxE1? MoXuNUBp Mo\GZoxw[2u|IITo[UBCT0VvQmPBMYlv\HWlZXSg[4Vv\S:ycn;0[YlvKGW6cILld5Nqd25ib3[gS3JRPzhib4KgR29ZNTJiKIC8NE4xPSl? NYWyNZczOjJ7OEKyNlg>
RAW264.7 MYDGeY5kfGmxbjDBd5NigQ>? M4f2dFE2KM7:TR?= NIjVfZoyPS1zMkCgcYlv MkTDZoxw[2u|IITo[UBxem:mdXP0bY9vKG:oIF7PMEBRT0V{LDDhcoQhXE6ILd8x NYO2TIc1OjJ5NEW1NlM>
RAW264.7 Mnu5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;jdHlSPS1|MDFOwG0> M4jtSVI1KGh? MUjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NGLPSYUzOjd2NUWyNy=>
HBL6 Mm\4RZBweHSxc3nzJGF{e2G7 NWfUW3N3OC53L{WvNlUh|ryP NGjVN243NzJ2IHi= NVTHR3B{\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFv\CCuZXXh[JMhfG9iYYDvdJRwe2m|IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{wrC= MlHQNlIxPzR6MkC=
HT29  M17OZ2Z2dmO2aX;uJGF{e2G7 MYexMVExKM7:TR?= NX;sSY9ROTEEoHi= NYPafZhvcW6lcnXhd4V{KEiRLUGgcXJPSSCjbnSgdJJwfGWrbjDlfJBz\XO|aX;u NGXu[3EzOTZ{MEm2OC=>
Ca9–22 Ml3IRZBweHSxc3nzJGF{e2G7 M4\yO|ExyqEQvF5CpC=> MofBNUBp NEK2dW5kd22ybHX0[Yx6KGmwaHnibZR{KEGOQT3QSHQucW6mdXPl[EBieG:ydH;zbZM> NEW1emczOTF|OES4NC=>
Ca9–22 NIS5WnpHfW6ldHnvckBCe3OjeR?= NVqwfJpSOTEEoN88UeKh M17uNFEhcA>? NIPjbFZkd22ybHX0[Yx6KGGkcn;nZZRmeyC2aHWgRWxCNVCGVD3pcoR2[2WmIFrOT{Bi[3SrdnH0bY9v NUD6eJpwOjFzM{i0PFA>
A-549 MojWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWKxNOKh|ryPwrC= M1;KTVI1NzR6IHi= MYXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NHfPUVAzODh4NkC0Ny=>
AP M4jXV2Z2dmO2aX;uJGF{e2G7 Mlr1OU8yOCEQvF2= NI[4SYw1QCCq NE\1V4Rld3ewcnXneYxifGW|IITo[UBDSURicILveIVqdiCuZY\lcEBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MUOyNFU6PjZ2NR?=
AQ1 MVnGeY5kfGmxbjDBd5NigQ>? M4S0N|UwOTBizszN M2\iS|Q5KGh? NXvEZoVT\G:5boLl[5Vt[XSnczD0bIUhSkGGIIDyc5RmcW5ibHX2[Ywh[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NF:yRVAzODV7Nk[0OS=>
AP MoP0SpVv[3Srb36gRZN{[Xl? MYqyNEDPxE1? NVLKTYtFPC96IHi= NWrZWZVL\G:5boLl[5Vt[XSnczD0bIUhSkGGIIDyc5RmcW5ibHX2[Ywh[SC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NXX0R5d{OjB3OU[2OFU>
AQ1 M{XabGZ2dmO2aX;uJGF{e2G7 M4HjfFIxKM7:TR?= NI\LcVk1NzhiaB?= NUDJfFV1\G:5boLl[5Vt[XSnczD0bIUhSkGGIIDyc5RmcW5ibHX2[Ywh[SC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MYWyNFU6PjZ2NR?=
THP-1 NYnNNHhQTnWwY4Tpc44hSXO|YYm= MlPiOeKh|ryPwrC= NGmxN3kxNjViaB?= NGXUOYVifHSnboXheIV{KHSqZTDMVHMucW6mdXPl[EBxNUoQulNOtUBxem:2ZXnuJIJ6KDd{JR?= NGHFe|UzODNyOUexPC=>
K562 Mm\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\UNk0{OCEQvF2= M4jN[|I1KGh? NVfkcJR2UUN3ME24JO69VSyrbnjpZol1eyClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NGK3ZpcyQTZ2NkiwOy=>
Jurket M3KxVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILQd4EzNTNyIN88US=> M3vFOVI1KGh? M4K5OmlEPTB;Nz6xJO69VSxiaX7obYJqfHNiY3XscEBoem:5dHigbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MWGxPVY1PjhyNx?=
U937 M2O3RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV:z[m9EOi1|MDFOwG0> NVfDWppbOjRiaB?= MnraTWM2OD1zMD61JO69VSxiaX7obYJqfHNiY3XscEBoem:5dHigbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MUOxPVY1PjhyNx?=
PBMC Ml;4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWWyMVMxKM7:TR?= MnfKNlQhcA>? MVrJR|UxRTRyLkKg{txONCCrbnjpZol1eyClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MkG2NVk3PDZ6MEe=
K562 NFfrWIVCeG:ydH;zbZMhSXO|YYm= NGm5T20zNTJywrFOwG3DqA>? M3\jNlI1KGh? NV7hN20xcW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JIFxd3C2b4Ppdy=> Mn6zNVk3PDZ6MEe=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP (see the Experimental section). The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP. The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

31332209 23441730
Growth inhibition assay
Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

31332209

Protocol

Cell Research:[2]
+ Expand
  • Cell lines: NCI-H1703 cells
  • Concentrations: ~8 μM
  • Incubation Time: 12 hours
  • Method: Cells are transfected with siRNA in 96-well microtiter plates and then cultured for 72 hours in complete NSCLC medium, treated with BAY 11-7082 for 12 hours. Cells are incubated with [3H]thymidine for 3 hours. The cells are collected on filters using an automatic cell harvester and radioactivity on the filters is measured by β-scintillation counting.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 41 mg/mL (197.82 mM)
Ethanol 10 mg/mL (48.25 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 207.25
Formula

C10H9NO2S

CAS No. 19542-67-7
Storage powder
in solvent
Synonyms BAY 11-7821

Bio Calculators

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID