BAY 11-7082

Catalog No.S2913 Synonyms: BAY 11-7821

BAY 11-7082 Chemical Structure

Molecular Weight(MW): 207.25

BAY 11-7082 is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. Also inhibiting components of the ubiquitin system.

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Cited by 96 Publications

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Choose Selective IκB/IKK Inhibitors

Biological Activity

Description BAY 11-7082 is a NF-κB inhibitor, inhibits TNFα-induced IκBα phosphorylation with IC50 of 10 μM in tumor cells. Also inhibiting components of the ubiquitin system.
Targets
E2-conjugating enzymes [6]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
10 μM
In vitro

BAY 11-7082 completely and specifically abrogates NF-κB DNA binding, downregulating the NF-κB-inducible cytokine IL-6 and inducing apoptosis. [1] BAY 11-7082 (< 8 μM) is able to effectively inhibit both basal and TNFα stimulated NFκB luciferase activity in a dose dependent manner. BAY 11-7082 (8 μM) strongly inhibits the rate of proliferation in NCI-H1703 cells. [2] Bay 11-7082 (5 μM) rapidly and efficiently reduces the DNA binding of NF-kappaB in HTLV-I-infected T-cell lines and down-regulates the expression of the antiapoptotic gene, Bcl-x(L), whereas it has little effect on the DNA binding of another transcription factor, AP-1. Bay 11-7082-induced apoptosis of primary ATL cells is more prominent than that of normal peripheral blood mononuclear cells, and apoptosis of these cells is also associated with down-regulation of NF-kappaB activity. Bay 11-7082 (5 μM) selectively induces apoptosis of HTLV-I–infected T-cell lines associated with down-regulation of the expression of cyclin D1, cyclin D2, and Bcl-xL. [3] BAY 11-7082 (100 μM) prevents the nuclear translocation of p65 elicited by NMDA and the NMDA-induced increase of NF-κB binding in mouse hippocampal slices. BAY 11-7082 prevents NMDA toxicity occurring in CA1 region of hippocampal slices with 40% neuroprotection at 20 μM and 70% neuroprotection at 100 μM. [4] BAY 11-7082 at all concentrations tested significantly inhibits NF-κB p65 DNA-binding activity in adipose tissue, whereas in skeletal muscle, BAY 11-7082 at 50 μM and 100 μM significantly inhibits NF-κB p65 DNA-binding activity. BAY 11-7082 (100 μM) reduces IKK-β protein in human adipose tissue and skeletal muscle. BAY 11-7082 (100 μM) significantly decreases the release of TNF-α from adipose tissue, whereas the release of IL-6 and IL-8 is significantly inhibited at all concentrations of BAY 11-7082 tested. BAY 11-7082 (50 μM) significantly decreases the release of TNF-α, IL-6, and IL-8 in skeletal muscle. [5] BAY 11-7082 is also found to inactivate the E2-conjugating enzymes Ubc (ubiquitin conjugating) 13 and UbcH7 and the E3 ligase LUBAC (linear ubiquitin assembly complex), and thus induces B-cell lymphoma and leukaemic T-cell death. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NYTmfYhUTnWwY4Tpc44hSXO|YYm= MYexNOKh|ryP NX7RVXNqOS53IHi= NYeyPYdE[WKxbHnzbIV{KEKSQTDpcoR2[2WmIIXwJJJm\3WuYYTpc44hd2ZiRl6gZY5lKE2PUD25xsA> MorpNlU4QTd2M{e=
SiHa  MoPPSpVv[3Srb36gRZN{[Xl? NHLjb5oyOMLizszN MXWxMlUhcA>? MkLsZYJwdGm|aHXzJGJRSSCrbnT1Z4VlKHWyIILl[5Vt[XSrb36gc4YhTk5iYX7kJG1OWC17wrC= MXSyOVc6PzR|Nx?=
ARPE-19 M{P6TmZ2dmO2aX;uJGF{e2G7 MVOxJO69VQ>? M{\BTlAvPSCq M2PrUpN2eHC{ZYPz[ZMhXEdvaX7keYNm\MLiSVytPOKheHKxbX;0[ZIh[WO2aY\heIlwdg>? NXzZcVJbOjV3OUOwNlk>
HCT116 NUO2VlhNTnWwY4Tpc44hSXO|YYm= Ml\IOUDPxE1? M2fXWVIhcA>? NHj2T4pFVVOR Ml6zZZR1\W63YYTld{B{cWy7bXHybY4ucW6mdXPl[EBld3ewcnXneYxifGmxbjDv[kBkgWOuaX6gSFE> M2Xp[VI2PDd7N{Kz
HMECs NF;2c3dHfW6ldHnvckBCe3OjeR?= NE\3So42yqEQvF2= Mn;6NkBp MofnZYJwdGm|aHXzJHRPTi4QsT3pcoR2[2WmIG\DRW0uOSCneIDy[ZN{cW:wwrC= MnzYNlUyQTNzMU[=
A549  NUjH[m1jTnWwY4Tpc44hSXO|YYm= M4PPNlEx6oDLwsXN MUexNkBp NFPJ[|F{fXCycnXzd4V{KES4bD2zJIlv\HWlZXSgZYN1cX[jdHnvckBw\iCyNkW= NFzsU5MzPTF3NkiwNC=>
RAW 264.7 M1jRcWZ2dmO2aX;uJGF{e2G7 M{HEelXDqM7:TR?= NHv1WlIyKGh? M1XXfIlvcGmkaYTzJHRPTi4QsTDhcoQhUUxvMUKgdFQxKHC{b3T1Z5Rqd25? MVOyOVAyQTV4Nx?=
macrophages MUHGeY5kfGmxbjDBd5NigQ>? NXX4VXJmPSEEtV2= M1LsS|MhcA>? NFfJXZNx[XK2aXHscJkh[myxY3vzJHlRTlNvaX7keYNm\CCneIDy[ZN{cW:wIH;mJIlPV1NiYX7kJGNQYC1{wrC= MkXDNlQ6Pjd6OUi=
HUVECs MYnGeY5kfGmxbjDBd5NigQ>? Mmm1N{0{OCEQvF2= NHnvWIwyKGh? MnLIdoVlfWOnczD0bIUh\XiycnXzd4lwdiCxZjDtbXIuOTR4YTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NIPUPXgzPDh4M{m2OS=>
HeLa  MmXlSpVv[3Srb36gRZN{[Xl? MoLoOeKh|ryP MkLNNlQhcA>? MnvySG1UVw>? MmPhdoVlfWOnczD0bIUh[WO2aY\peJkhd2ZiVF7GMe6yKHC{b33veIVzyqB? M{LSXFI1PjV5N{iz
A549 NYPv[pBETnWwY4Tpc44hSXO|YYm= NYH3UpB3OTBizszN M{PBRVHjiImq Ml\SbY5pcWKrdIOgeIhmKGmwY4LlZZNmKG:oIIDoc5NxcG9vSd86Ru6yKGmwIGDBNVA{NWmwZnXjeIVlKGO3bIT1doV{yqB? NF7XN2szPDZzMkS4PC=>
HUVEC M4PlbWZ2dmO2aX;uJGF{e2G7 M{i3RlIxKML3TR?= MoHpNE42KGh? MUHEUXNQ MmjMdJJmfmWwdIOgeIhmKGmwZIXjeIlwdiCxZjDFRW0h\XiycnXzd4lwdg>? NV71O5lROjR3NUGyNFk>
A549RT-eto NUPTcXBwSXCxcITvd4l{KEG|c3H5 NHXMOYgyOMLizszN M1\SVlI1KGh? NXzLWXhRTE2VTx?= NGDSbnZi[2OnbHXyZZRmeyCIRWLPMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NFW4fG4zPDV|NUC4Ny=>
THP-1  MWTGeY5kfGmxbjDBd5NigQ>? MUmwMlEwOcLizszN NGWyfoQxNjViaB?= MnOwZYJzd2ejdHXzJHRPTi4QsTDz[YNz\XSrb36gZZMhf2WubDDhd{B1cGViaX7jdoVie2WmIIPlZ5JmfGmxbjDv[kBKVC14IHHu[EBKVC1zzsK= NVOybWRxOjR|N{i1N|Y>
SKCXCR2  MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPyfoIzKML3TR?= NXj4OJE6PDhiaB?= NVK0SIVr\GWlcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdiC|aXfubYZq[2GwdHz5 MV:yOFM4Pjd2Nx?=
SKCXCR2  NFqwfVdHfW6ldHnvckBCe3OjeR?= NYDuZlZSOiEEtV2= NXTDfnRvPDhiaB?= MljtZoxw[2u|IITo[UBEYEOOMT3pcoR2[2WmIHPlcIwhcW64YYPpc44> NEXjZ5QzPDN5Nke0Oy=>
OVCXCR2 M1jnfGZ2dmO2aX;uJGF{e2G7 NFnBTXEzKML3TR?= NVO2WW85PDhiaB?= M{H1SYJtd2OtczD0bIUhS1iFTEGtbY5lfWOnZDDj[YxtKGmwdnHzbY9v NFzTcokzPDN5Nke0Oy=>
DSCs  NFPVNW1HfW6ldHnvckBCe3OjeR?= NHPaOIQzNjVizszN NXS4WGo3OC53IHi= M2PQXpJmfmW{c3XzJJRp\SCnbnjhcoNmdWWwdDDv[kBES0x{L1PDVlIh\XiycnXzd4lwdiCxZjDEV2N{KGmwZIXj[YQh[nliSVytN|M> M2S2R|I1OzR2MkSw
WPs NXT1PYZKTnWwY4Tpc44hSXO|YYm= MXiyOUDPxE1? NHG5cWQ2KG2rbh?= NVLjUIlMe3WycILld5NmeyCDVGCgZY5lKH[ZRjDz[YNz\XSrb36= M4\YTlI1OzNzMkC3
A549RT-eto NFXPSFBCeG:ydH;zbZMhSXO|YYm= NVvsOGJQOTEEoN88US=> Mli5NlQhcA>? MmK0ZYNk\WyncnH0[ZMhTjF2IHX4eJJi[3RvbXXkbYF1\WRiYYDvdJRwe2m|IHPvcYJqdmWmIITy[YF1dWWwdDD3bZRpKEZzNB?= MnK1NlQzOjB5MkW=
A549RT-eto MVjGeY5kfGmxbjDBd5NigQ>? NWXwNmt2OTEEoN88US=> Ml[3NlQhcA>? MkXC[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDOSk3PwkJiYX7kJHAu\3EEoB?= NEPaUVAzPDJ{MEeyOS=>
FaDu MnnHSpVv[3Srb36gRZN{[Xl? NFfwOZgzKGh? NFrWWm9qdmirYnn0d{BxPjViZYjwdoV{e2mxbjDhcoQh[myxY3vzJHRPTs7zLXnu[JVk\WRiVGfJV3Qh\XiycnXzd4lwdg>? NIDNRY8zPDJ{ME[yNi=>
IVD  MkHnSpVv[3Srb36gRZN{[Xl? MmXoNVDDqM7:TR?= NVnFOok{OyCm M4PkO5JmfmW{c3XzJHRPTi4QsfMAl41m\GmjdHXkJJN2eHC{ZYPzbY9vKG:oIITo[UBlcXOlIH3heJJqgCCvYXPyc41wdGWldXzld{Bi\2e{ZXPhckBidmRiY3;scIFo\W5iSVm= MmLFNlQyPzZ6MEi=
IVD  M2PUWWZ2dmO2aX;uJGF{e2G7 NI\XS4MyOMLizszN Ml7tN{Bl NWnLRZI6[WK{b3fheIV{KFSQRj5OtgKBm2mwZIXj[YQhfXBvcnXneYxifGmxbjDv[kBCTEGPVGOtOEBidmRiQVTBUXRUNTYEoB?= NX6yUpNtOjRzN{[4NFg>
iNKT M{H5cmZ2dmO2aX;uJGF{e2G7 NV3SSIU{OTBxMUCwJO69VQ>? M{[0Z|AvPSCq NHj5OIpqdmirYnn0d{B1cGViaX7keYN1cW:wIH;mJGEzSVJibWLORUBidmRib4To[ZIh\mGldH;y NGOxPZEzPDF{NES1Ny=>
PC-3 MmLoSpVv[3Srb36gRZN{[Xl? NX\CVmFDOi53L{WvNVAh|ryP NYmy[XJzOC53IHi= NVr3cXBE[myxY3vzJGlITi2LST3pcoR2[2WmIGPUV{BuWk6DIHX4dJJme3Orb39CpC=> M1zjVVI1ODV3NUKw
THP-1  MlPpSpVv[3Srb36gRZN{[Xl? NGXF[lAyOMLizszN MXGxxsBp NX7CcmJr[WKxbHnzbIV{KHSqZTDl[oZm[3Rib3[gdmhUWDJ5IH;uJHNTNUFibWLORS=> M17IVVI{QTN7M{m4
A549 MVjGeY5kfGmxbjDBd5NigQ>? NYTvOm93OcLizszN NWHsb3NqPDhiaB?= NUDCW|Ay\W6qYX7j[ZMhfGinIIXwMZJm\3WuYYTpc44hd2ZiSd86RkBidmRic4Xid4VyfWWwdDDk[YNz\WG|ZTDpckBD[XhiZYjwdoV{e2mxbjDpcoR2[2WmIHL5JINwdWKrbnXkJJN1cW23bHH0bY9v NYf2TVlwOjN7MECwPFA>
A549 M4nGOWFxd3C2b4Ppd{BCe3OjeR?= M4jQRlHDqM7:TR?= MVq0PEBp NG[3ZZFz\WS3Y3XzJJRp\SClZXzsJIRm[XSqIHnu[JVk\WRiYomgZ49u[mmwZXSgd5RqdXWuYYTpc44> MYOyN|kxODB6MB?=
NCI-N87 NHTndHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\IbFExNzJyL{OwJO69VQ>? MoHLOk8zPCCq NIPIflR{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JJNq\26rZnnjZY51dHl? MXuyN|g1PjV2NR?=
AGS NGfjWGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW[yV3pKOTBxMkCvN|Ah|ryP M2XnZVYwOjRiaB?= NWft[3hNe3WycILld5NmeyClZXzsJJZq[WKrbHn0fUB{cWewaX\pZ4FvfGy7 NGHYfZEzOzh2NkW0OS=>
MGC80-3 NIj0WXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWSxNE8zOC9|MDFOwG0> NYHncJBzPi9{NDDo M4rEU5N2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? MWKyN|g1PjV2NR?=
HGC-27  NFqyTJdHfW6ldHnvckBCe3OjeR?= M4XBSlcvPS9zNT:zNEDPxE1? NGq0XFc3KGh? MUfpcoR2[2W|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBidmRidYCtdoVofWyjdHnvckBw\iCLzsrC{tE> MVmyN|g1PjV2NR?=
MGC80-3 MojLSpVv[3Srb36gRZN{[Xl? MmLNO{42NzF3L{OwJO69VQ>? NYH1bXNbPiCq MXPpcoR2[2W|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBidmRidYCtdoVofWyjdHnvckBw\iCLzsrC{tE> M1XKWVI{QDR4NUS1
HGC-27  M1rZSmFxd3C2b4Ppd{BCe3OjeR?= MmfXO{42NzF3L{OwJO69VQ>? MkSyOkBp MV7pcoR2[2W|IHHwc5B1d3OrczDpckBiKHSrbXWtJIFv\CCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NGj3[FYzOzh2NkW0OS=>
HBE  NGfyN2pHfW6ldHnvckBCe3OjeR?= NEPoPVUyOM7:TR?= MojVN4g> NV3vb4lI[WKxbHnzbIV{KHSqZTDpcoNz\WG|ZYOgc4YhUUxvNjDlfJBz\XO|aX;uJIlv\HWlZXSgZpkhS1OH MWqyN|gzPDB6OR?=
HepG2 NGrEfIlHfW6ldHnvckBCe3OjeR?= NUH1c4dIOC5|L{GvN{DPxE1? MV20PEBp M4PhZpJm\HWlZYOgTWw3NWmwZIXj[YQhWE:QMTDlfJBz\XO|aX;u MYWyN|c6OTh|Mx?=
THP-1 NIj1e4NHfW6ldHnvckBCe3OjeR?= MYK1JOK2VQ>? NIn3VW0yKGh? NUniVmFRTE2VTx?= NGDXVpFqdmirYnn0d{BOXEJvaX7keYNm\CCQRt86RkBi[3SrdnH0bY9v M12yeVI{PjN2MkG4
THP-1  M4G4Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkD5OUDDvU1? NXTKTnpNPC96IHS= NXvDbpJQTE2VTx?= NHS2Sm1z\WS3Y3XzJJRp\SC4aXHibYxqfHlib3[gbY51emGlZXzseYxiesLiTWTC NGrLemgzOzZ|NEKxPC=>
MDM M1vnc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV:1JOK2VQ>? NUXQe3FkPC96IHS= MWfEUXNQ NUG1PYtCemWmdXPld{B1cGVidnnhZoltcXS7IH;mJIlvfHKjY3XscJVt[XMEoF3URi=> NVXs[|huOjN4M{SyNVg>
AM MmPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljLOUDDvU1? MYW0M|gh\A>? NYjaWGlFTE2VTx?= MV;y[YR2[2W|IITo[UB3cWGkaXzpeJkhd2ZiaX70doFk\WyudXzhduKhVVSE M33HXVI{PjN2MkG4
RAW 264  MWnGeY5kfGmxbjDBd5NigQ>? M1nm[lAvOi13INM1US=> M2XiVVMxNzZyL{mwJI1qdg>? NFLrfllqdmirYnn0d{B1cGVicHjvd5Bp[XSjc3WgZYN1cX[rdImgc4YhWFSSMVNCpC=> MWqyN|U4QDNyMh?=
HUVEC MoT6SpVv[3Srb36gRZN{[Xl? MXqxNOKh|ryP NWnJbot2OC53IHi= NXz3OmJjTE2VTx?= NWXVTG1i[2:3boTldoFkfGW|IITo[UBtd3O|IH;mJHRq\TJibWLORS=> MmfmNlM2PjN4M{K=
HT29 NUW5d3BQTnWwY4Tpc44hSXO|YYm= M3vCTlExNzNyL{GwNEDPxE1? M3\Jb|EhcA>? NG\GfmRqdmirYnn0[ZMh[m:2aDDUW2VCUy2rbnT1Z4VlKHBzMECgdJJw[2W|c3nu[y=> NEHLRpkzOzV{N{G1OC=>
HT29 NXLGV|FVTnWwY4Tpc44hSXO|YYm= M4HxN|ExNzNyL{GwNEDPxE1? NWrONWhHOSCq MkX1bY5pcWKrdIOgWG5HNWmwZIXj[YQheGixc4Doc5J6dGG2aX;uJIFv\CCmZXfyZYRifGmxbjDv[kBK|rqEzsG= M4fFTlI{PTJ5MUW0
MM.1S Mn;VRZBweHSxc3nzJGF{e2G7 M3TxeVMxKML3TR?= MVezJIg> MVPpcoR2[2W|IF3NJINmdGxiZHXheIghcW64b3z2[ZMhdmWlcn;zbZM> MU[yN|UzPzF3NB?=
KMS-12-BM MYPBdI9xfG:|aYOgRZN{[Xl? MmnPN|AhyrWP M3jJeVMhcA>? M1G3WIlv\HWlZYOgUW0h[2WubDDk[YF1cCCrbo\vcJZmeyCwZXPyc5Nqew>? NGfONWEzOzV{N{G1OC=>
BAFs MXHGeY5kfGmxbjDBd5NigQ>? MW[wMlUwOcLizszN M1LFT|I1KGh? MYXpcohq[mm2czDUUmbPuS:GRWigbY5lfWOnZDDDXXAyQUFzwrD0doFve2O{aYD0dy=> MWqyN|Q5PTR3Nx?=
SP6.5 MYHGeY5kfGmxbjDBd5NigQ>? MYO1JO69VQ>? MkC1NkBp Mmfz[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh M1XIcVI{PDR|MEi2
VUP NXvGfYJuTnWwY4Tpc44hSXO|YYm= MUS1JO69VQ>? MVWyJIg> M3;iSYRm[3KnYYPld:KhfHKjboPsc4NifGmxbjDv[kBxPjViaX6geIhmKG63Y3zleZPDqA>? MXuyN|Q1OzB6Nh?=
OCM1 M1iwfWZ2dmO2aX;uJGF{e2G7 MoXOOUDPxE1? NGHN[YIzKGh? MlnK[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh M{DMRlI{PDR|MEi2
OM431 MVLGeY5kfGmxbjDBd5NigQ>? NGLRS2M2KM7:TR?= M2DWUVIhcA>? Mljl[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh NWHKTphvOjN2NEOwPFY>
SP6.5 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HMU|IvPS1{MDFOwG0> M{DnfVI1KGkEoB?= MVvJR|UxRTVizszNMEBmgGirYnn0d{B{fHKxbnegZY51cS2ycn;sbYZmemG2aY\lJIVn\mWldIOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M{DGR|I{PDR|MEi2
VUP MojmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTHNk42NTJyIN88US=> M4XBeFI1KGkEoB?= NF;nUW5KSzVyPUWg{txONCCneHjpZol1eyC|dILvcoch[W62aT3wdo9tcW[ncnH0bZZmKGWoZnXjeJMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MkjJNlM1PDNyOE[=
OCM1 NV7QR5JjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHu3SIozNjVvMkCg{txO NVfi[JFKOjRiaNMg MkLXTWM2OD13IN88UUwh\XiqaXLpeJMhe3S{b37nJIFvfGlvcILvcIln\XKjdHn2[UBm\m[nY4TzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NHPrUYUzOzR2M{C4Oi=>
OM431 Mn\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;ZbGUzNjVvMkCg{txO M{nIRlI1KGkEoB?= NU\ZUpBiUUN3ME21JO69VSxiZYjobYJqfHNic4Tyc45oKGGwdHmtdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NFexe28zOzR2M{C4Oi=>
SP6.5 NE\vfnhCeG:ydH;zbZMhSXO|YYm= Mnj5OUDPxE1? NETuemszPCCqwrC= MWXpcoR2[2W|IHHwc5B1d3Orc9Mg MVOyN|Q1OzB6Nh?=
VUP M3HqbmFxd3C2b4Ppd{BCe3OjeR?= MWO1JO69VQ>? M4jlUVI1KGkEoB?= M{j6dolv\HWlZYOgZZBweHSxc3nzxsA> M37lXFI{PDR|MEi2
OCM1 MXXBdI9xfG:|aYOgRZN{[Xl? NXnGTotuPSEQvF2= MoXtNlQhcMLi NU\ibJQ6cW6mdXPld{BieG:ydH;zbZPDqA>? Mm\YNlM1PDNyOE[=
OM431 M2fjPWFxd3C2b4Ppd{BCe3OjeR?= NYP4RYVsPSEQvF2= NVHhT3R{OjRiaNMg NF;j[GZqdmS3Y3XzJIFxd3C2b4Ppd:Kh NE[ybXQzOzR2M{C4Oi=>
SP6.5 NVTMOW9tTnWwY4Tpc44hSXO|YYm= MYW1JO69VQ>? Mkm4NVIhcA>? M1PIZpJm\HWlZYOgeIhmKG2rZ4LheIlwdsLi Mni5NlM1PDNyOE[=
VUP M{PvbWZ2dmO2aX;uJGF{e2G7 NG\ZeZY2KM7:TR?= NWHQVpB6OTJiaB?= M4G4U5Jm\HWlZYOgeIhmKG2rZ4LheIlwdsLi NUnRU|I6OjN2NEOwPFY>
OCM1 MWTGeY5kfGmxbjDBd5NigQ>? NUDpPXp4PSEQvF2= NI\n[YwyOiCq Mm\tdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? M13jWlI{PDR|MEi2
OM431 MXrGeY5kfGmxbjDBd5NigQ>? NGPVRo02KM7:TR?= NVv2XnhYOTJiaB?= NFjEO5Nz\WS3Y3XzJJRp\SCvaXfyZZRqd28EoB?= Mlr1NlM1PDNyOE[=
HBL-1  NIj6THRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXiWY8{yqEQvF2= MlnXNlQwPDhxN{KgbC=> NETpTHdFVVOR NILXWmp{dG:5czDj[YxtKGe{b4f0bEBud2Snc4TsfS=> MX:yN|Q1OTd|MB?=
RAW 264.7 M1;UTmZ2dmO2aX;uJGF{e2G7 NEDEOI8zNTF3IN88US=> MlvVNUBp MVrEUXNQ NVj2cWtze3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCLS1ug[oFucWy7IH3lcYJmenN? NFL4VlgzOzR2MUezNC=>
IL-1R  MXXGeY5kfGmxbjDBd5NigQ>? M3;5d|IuOTVizszN NED6XokyKGh? MWDEUXNQ MlLad5VxeHKnc4Pld{B1cGViYXP0bZZifGmxbjDv[kBKU0tiZnHtbYx6KG2nbXLldpM> NFn2XYwzOzR2MUezNC=>
RAW 264.7 NHXIWm9HfW6ldHnvckBCe3OjeR?= MYOxOUDPxE1? Mn\jNUBp Ml7rSG1UVw>? M2HBSZN2eHC{ZYPz[ZMhfGinIHHjeIl3[XSrb36gc4Yh[W6mIFrOTy=> M{XYUVI{PDRzN{Ow
IL-1R  NXXnOY95TnWwY4Tpc44hSXO|YYm= M4LzOFE2KM7:TR?= MYGxJIg> NVH3VGpoTE2VTx?= NX:4bpNue3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCjbnSgTm5M MUeyN|Q1OTd|MB?=
U2OS M3\oSWZ2dmO2aX;uJGF{e2G7 NX\aWXljOTVizszN NV62fZA6OSCq MX\EUXNQ MUPwdoV3\W62czD0bIUhVFCVLTDvdkBKVC1zLYP0bY12dGG2ZXSg[o9zdWG2aX;uJI9nKEt4Mz3wWYIh[2ijaX7zxsA> M4nDelI{PDRzN{Ow
MT‐1  MYfGeY5kfGmxbjDBd5NigQ>? MUK4xsDDvW1? MVGzJIg> NWWxbGNN\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdQKBmFOWQWSzJIFv\CCy4pEQOGVDWDF? MonINlMzPzh2N{m=
MT‐2  MWDGeY5kfGmxbjDBd5NigQ>? NVKzSI94QMLiwsXt NYTs[VZwOyCq NVGyTFZo\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdQKBmFOWQWSzJIFv\CCy4pEQOGVDWDF? NH;NWG4zOzJ5OES3PS=>
MT‐1  MYLGeY5kfGmxbjDBd5NigQ>? Ml3CPOKhyrWv NUPpUpVpOyCq Mlru[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZidHjlJJA3PSC|dXL1col1KG:oIF7G5qCR|rqEwrC= MYiyN|I4QDR5OR?=
MT‐2  NHvrdllHfW6ldHnvckBCe3OjeR?= M2HNbVjDqML3bR?= M2DR[|MhcA>? NYe5PVY3\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[geIhmKHB4NTDzeYJ2dmm2IH;mJG5H6oDSzsrCxsA> MVqyN|I4QDR5OR?=
MCF-7  NG\R[|hHfW6ldHnvckBCe3OjeR?= M332WVIvPS1zNTFOwG0> MWKwMlUhcA>? MXvEUXNQ MWTjZZV{\XNidHjlJIdz[WS3YXygcI9{eyCxZjDj[YxtKGGmaHXzbY9v M3vGSVI{ODl|MkK3
HaCaT  NITQe3JHfW6ldHnvckBCe3OjeR?= MmjwOU4xyqEQvF2= NHjnZmkyyqCqwrC= NWfE[nJ1[XS2ZX71ZZRmeyC2aHWgWGNQUC2rbnT1Z4VlKHC{b3T1Z5Rqd25ib3[gTWwuPsLi M{\He|I{ODRzMU[4
A549  M2[wRWZ2dmO2aX;uJGF{e2G7 NHy5TYIyKGh? NVrTVWhFcW6qaXLpeJMhVFSDLXnu[JVk\WRiU2CtRUBuWk6DIIDyc4R2[3Srb39CpJNq\26rZnnjZY51dHl? NWPBbJR2OjNyM{GyNVM>
OA chondrocytes  NVrobGVlTnWwY4Tpc44hSXO|YYm= NEjUTIUyOCEQvF2= MoD1NUBp NGn1OnhjdG:la4OgeIhmKEGJRT3CV2EucW6mdXPl[EBo\W6nL4Dyc5RmcW5iZYjwdoV{e2mxbjDv[kBIWlB5ODDvdkBEV1hvMjCodFwxNjB3KR?= M4i0U|IzQTh{MkK4
RAW264.7 MniwSpVv[3Srb36gRZN{[Xl? NIDYOpkyPSEQvF2= M2DP[lE2NTF{MDDtbY4> M2nxOoJtd2OtczD0bIUheHKxZIXjeIlwdiCxZjDOU{whWEeHMjygZY5lKFSQRj5OtS=> MnqwNlI4PDV3MkO=
RAW264.7 M2\5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHtSFc2NTNyIN88US=> NXHsWGdbOjRiaB?= MWfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NIDDdnkzOjd2NUWyNy=>
HBL6 M2Hkd2Fxd3C2b4Ppd{BCe3OjeR?= M1K2OlAvPS93L{K1JO69VQ>? NU\K[WVoPi9{NDDo MonM[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHHu[EBt\WWjZIOgeI8h[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzyqB? M1vjXlIzODd2OEKw
HT29  NGHEWlhHfW6ldHnvckBCe3OjeR?= M1L0TFEuOTBizszN M{\ZVlExyqCq Mn\vbY5kemWjc3XzJGhQNTFibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:w MWSyNVYzODl4NB?=
Ca9–22 M4DIOmFxd3C2b4Ppd{BCe3OjeR?= MVyxNOKh|ryPwrC= NH\NZYcyKGh? MnfjZ49ueGyndHXsfUBqdmirYnn0d{BCVEFvUFTUMYlv\HWlZXSgZZBweHSxc3nz MVuyNVE{QDR6MB?=
Ca9–22 NH65bYpHfW6ldHnvckBCe3OjeR?= MWixNOKh|ryPwrC= MYqxJIg> NE[wcHVkd22ybHX0[Yx6KGGkcn;nZZRmeyC2aHWgRWxCNVCGVD3pcoR2[2WmIFrOT{Bi[3SrdnH0bY9v MXyyNVE{QDR6MB?=
A-549 MojPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1:zfFExyqEQvF5CpC=> NHzOZVYzPC92ODDo NXnFOY1DcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NUTVelhSOjB6Nk[wOFM>
AP MXvGeY5kfGmxbjDBd5NigQ>? MXe1M|ExKM7:TR?= NYPYSXBlPDhiaB?= MWHkc5dvemWpdXzheIV{KHSqZTDCRWQheHKxdHXpckBt\X[nbDDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NFnOToozODV7Nk[0OS=>
AQ1 NX\Zfm55TnWwY4Tpc44hSXO|YYm= NITndow2NzFyIN88US=> MnjBOFghcA>? M3XaOIRwf26{ZXf1cIF1\XNidHjlJGJCTCCycn;0[YlvKGyndnXsJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MUOyNFU6PjZ2NR?=
AP M3LZ[mZ2dmO2aX;uJGF{e2G7 NYHGZ2VwOjBizszN NILicHo1NzhiaB?= NWXiTHFj\G:5boLl[5Vt[XSnczD0bIUhSkGGIIDyc5RmcW5ibHX2[Ywh[SC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MkfBNlA2QTZ4NEW=
AQ1 M1rnTmZ2dmO2aX;uJGF{e2G7 M1nqRlIxKM7:TR?= NELqOYw1NzhiaB?= MlGz[I94dnKnZ4XsZZRmeyC2aHWgRmFFKHC{b4TlbY4hdGW4ZXygZUB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NH3qR3gzODV7Nk[0OS=>
THP-1 MnPXSpVv[3Srb36gRZN{[Xl? NGn2OIc2yqEQvF5CpC=> MWmwMlUhcA>? M3X5NYF1fGWwdXH0[ZMhfGinIFzQV{1qdmS3Y3XkJJAuUc78Qt8xJJBzd3SnaX6gZpkhPzJn M3XBO|IxOzB7N{G4
K562 M332V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HWZVIuOzBizszN MVuyOEBp Mn[zTWM2OD16IN88UUxqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NX:z[o41OTl4NE[4NFc>
Jurket NGC2[ZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4P0S|IuOzBizszN NXS5UHRKOjRiaB?= MkXvTWM2OD15LkGg{txONCCrbnjpZol1eyClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NYXpNHpDOTl4NE[4NFc>
U937 Mnf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LIOVIuOzBizszN NXHibmNiOjRiaB?= M4WzPGlEPTB;MUCuOUDPxE1uIHnubIljcXS|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M{DPfVE6PjR4OEC3
PBMC NWOyd|J3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXWNk0{OCEQvF2= Mn7INlQhcA>? M2e5NmlEPTB;NECuNkDPxE1uIHnubIljcXS|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MY[xPVY1PjhyNx?=
K562 MWXBdI9xfG:|aYOgRZN{[Xl? NIiwUFkzNTJywrFOwG3DqA>? M{fnVFI1KGh? NYfTWlhpcW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JIFxd3C2b4Ppdy=> Mn7SNVk3PDZ6MEe=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP (see the Experimental section). The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP. The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

31332209 23441730
Growth inhibition assay
Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

31332209

Protocol

Cell Research:[2]
- Collapse
  • Cell lines: NCI-H1703 cells
  • Concentrations: ~8 μM
  • Incubation Time: 12 hours
  • Method: Cells are transfected with siRNA in 96-well microtiter plates and then cultured for 72 hours in complete NSCLC medium, treated with BAY 11-7082 for 12 hours. Cells are incubated with [3H]thymidine for 3 hours. The cells are collected on filters using an automatic cell harvester and radioactivity on the filters is measured by β-scintillation counting.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 41 mg/mL (197.82 mM)
Ethanol 10 mg/mL (48.25 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+corn oil
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 207.25
Formula

C10H9NO2S

CAS No. 19542-67-7
Storage powder
in solvent
Synonyms BAY 11-7821

Bio Calculators

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID