Bay 11-7085

Catalog No.S7352 Synonyms: Bay 11-7083

For research use only.

BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.

Bay 11-7085 Chemical Structure

CAS No. 196309-76-9

Selleck's Bay 11-7085 has been cited by 34 publications

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Biological Activity

Description BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Features Selective IκBα phosphorylation inhibitor.
IκBα phosphorylation [1]
10 μM
In vitro

BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells. [1] BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel. [3] Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NH;lc4tHfW6ldHnvckBie3OjeR?= NF3GUYxKdmirYnn0bY9vKG:oIFnMMVEu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBqdiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIJtd2OtaX7nJI9nKHB3MD;wOlUhdnWlbHXhdkB1emGwc3zvZ4F1cW:wLDDJR|UxRTBwMTFOwG0> NI\hc2wyPzh2NUi1NC=>
human A2780 cells MmnuSpVv[3Srb36gZZN{[Xl? MVrBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDMke4NEBk\WyuczDjc{1kfWy2dYLl[EB4cXSqIH3ld491cGWuaXHsJINmdGxibX;uc4xigWW{czDifUBncXKnZnz5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0zNjZizszN MnvUNlI{OjZ|OUW=
human BxPC3 cells NX\FN3ZvS3m2b4TvfIlkcXS7IHHzd4F6 M{nQe|QhcA>? M1S2ZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJ5WEN|IHPlcIx{KGOxLXP1cJR2emWmIIfpeIghcHWvYX6gcYV{d3SqZXzpZYwh[2WubIOgZYZ1\XJiNDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFXDOVA:Oy53IN88US=> Mlr1NlU2QDF{NkG=
human SKOV3 cells NGDlUVNEgXSxdH;4bYNqfHliYYPzZZk> NEW5Wm01KGh? M1\i[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z|IHPlcIx{KGOxLXP1cJR2emWmIIfpeIghcHWvYX6gcYV{d3SqZXzpZYwh[2WubIOgZYZ1\XJiNDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFXDOVA:PSEQvF2= MUmyOVU5OTJ4MR?=
human SU86 cells NGD2UmdEgXSxdH;4bYNqfHliYYPzZZk> MUm0JIg> M1nzd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNWQDZiY3XscJMh[29vY4XseJVz\WRid3n0bEBpfW2jbjDt[ZNwfGinbHnhcEBk\WyuczDh[pRmeiB2IHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2ViYYPzZZkhemWuYYTpeoUhfG9iY3;ueJJwdCxiRVO1NF02NjlizszN NVTsNJRwOjV3OEGyOlE>
human HT-29 cells NGO2cVFHfW6ldHnvckBie3OjeR?= MV:zNEBucW6| NF7zRYVT\WS3Y4Tpc44hd2ZiY3XscJVt[XJiR2PIJIxmfmWuIHnuJIh2dWGwIFjUMVI6KGOnbHzzJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc{1kfWy2dYLpcochf2m2aDDoeY1idiCvZYPveIhmdGmjbDDj[YxteyCvZXHzeZJm\CCjZoTldkA2KGi{czDifUBncXKnZnz5JIx2[2moZYLhd4Uh[XO|YYm= NXq2em9WOjV3OEGyOlE>
In vivo BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation. [2] BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. [3]

Protocol (from reference)

Kinase Assay:[1]
  • In Gel Kinase Assay:

    In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.

Cell Research:[1]
  • Cell lines: HUVEC cells
  • Concentrations: ~20 μM
  • Incubation Time: 16 hours
  • Method: Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.
Animal Research:[2]
  • Animal Models: Rat model of pneumococcal meningitis
  • Dosages: PBS
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 249.33


CAS No. 196309-76-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)C=CC#N

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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