Bay 11-7085

Catalog No.S7352 Synonyms: Bay 11-7083

For research use only.

BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.

Bay 11-7085 Chemical Structure

CAS No. 196309-76-9

Selleck's Bay 11-7085 has been cited by 34 publications

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Biological Activity

Description BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Features Selective IκBα phosphorylation inhibitor.
Targets
IκBα phosphorylation [1]
10 μM
In vitro

BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells. [1] BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel. [3] Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NH;lc4tHfW6ldHnvckBie3OjeR?= NF3GUYxKdmirYnn0bY9vKG:oIFnMMVEu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBqdiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIJtd2OtaX7nJI9nKHB3MD;wOlUhdnWlbHXhdkB1emGwc3zvZ4F1cW:wLDDJR|UxRTBwMTFOwG0> NI\hc2wyPzh2NUi1NC=>
human A2780 cells MmnuSpVv[3Srb36gZZN{[Xl? MVrBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDMke4NEBk\WyuczDjc{1kfWy2dYLl[EB4cXSqIH3ld491cGWuaXHsJINmdGxibX;uc4xigWW{czDifUBncXKnZnz5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0zNjZizszN MnvUNlI{OjZ|OUW=
human BxPC3 cells NX\FN3ZvS3m2b4TvfIlkcXS7IHHzd4F6 M{nQe|QhcA>? M1S2ZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJ5WEN|IHPlcIx{KGOxLXP1cJR2emWmIIfpeIghcHWvYX6gcYV{d3SqZXzpZYwh[2WubIOgZYZ1\XJiNDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFXDOVA:Oy53IN88US=> Mlr1NlU2QDF{NkG=
human SKOV3 cells NGDlUVNEgXSxdH;4bYNqfHliYYPzZZk> NEW5Wm01KGh? M1\i[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z|IHPlcIx{KGOxLXP1cJR2emWmIIfpeIghcHWvYX6gcYV{d3SqZXzpZYwh[2WubIOgZYZ1\XJiNDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIHHzd4F6KHKnbHH0bZZmKHSxIHPvcpRzd2xuIFXDOVA:PSEQvF2= MUmyOVU5OTJ4MR?=
human SU86 cells NGD2UmdEgXSxdH;4bYNqfHliYYPzZZk> MUm0JIg> M1nzd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNWQDZiY3XscJMh[29vY4XseJVz\WRid3n0bEBpfW2jbjDt[ZNwfGinbHnhcEBk\WyuczDh[pRmeiB2IHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2ViYYPzZZkhemWuYYTpeoUhfG9iY3;ueJJwdCxiRVO1NF02NjlizszN NVTsNJRwOjV3OEGyOlE>
human HT-29 cells NGO2cVFHfW6ldHnvckBie3OjeR?= MV:zNEBucW6| NF7zRYVT\WS3Y4Tpc44hd2ZiY3XscJVt[XJiR2PIJIxmfmWuIHnuJIh2dWGwIFjUMVI6KGOnbHzzJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc{1kfWy2dYLpcochf2m2aDDoeY1idiCvZYPveIhmdGmjbDDj[YxteyCvZXHzeZJm\CCjZoTldkA2KGi{czDifUBncXKnZnz5JIx2[2moZYLhd4Uh[XO|YYm= NXq2em9WOjV3OEGyOlE>
In vivo BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation. [2] BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. [3]

Protocol (from reference)

Kinase Assay:[1]
  • In Gel Kinase Assay:

    In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.

Cell Research:[1]
  • Cell lines: HUVEC cells
  • Concentrations: ~20 μM
  • Incubation Time: 16 hours
  • Method: Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.
Animal Research:[2]
  • Animal Models: Rat model of pneumococcal meningitis
  • Dosages: PBS
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 249.33
Formula

C13H15NO2S

CAS No. 196309-76-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)C=CC#N

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