Bay 11-7085

For research use only.

Catalog No.S7352 Synonyms: Bay 11-7083

22 publications

Bay 11-7085 Chemical Structure

Molecular Weight(MW): 249.33

BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.

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Selleck's Bay 11-7085 has been cited by 22 publications

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Biological Activity

Description BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Features Selective IκBα phosphorylation inhibitor.
IκBα phosphorylation [1]
10 μM
In vitro

BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells. [1] BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel. [3] Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells M2nseGZ2dmO2aX;uJIF{e2G7 M2TEXmlvcGmkaYTpc44hd2ZiSVytNU1idHCqYT3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgZoxw[2urbnegc4YheDVyL4C2OUBvfWOuZXHyJJRz[W6|bH;jZZRqd25uIFnDOVA:OC5zIN88US=> Mn3hNVc5PDV6NUC=
human A2780 cells NVruOlVPTnWwY4Tpc44h[XO|YYm= MmHERY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUK3PFAh[2WubIOgZ48u[3WudIXy[YQhf2m2aDDt[ZNwfGinbHnhcEBk\WyuIH3vco9t[XmncoOgZpkh\mm{ZX\sfUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:Oi54IN88US=> MY[yNlMzPjN7NR?=
human BxPC3 cells NV64TI5qS3m2b4TvfIlkcXS7IHHzd4F6 MkXSOEBp NIHSNGhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDgFCFMzDj[YxteyClbz3jeYx1fXKnZDD3bZRpKGi3bXHuJI1me2:2aHXsbYFtKGOnbHzzJIFnfGW{IESgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG|ZTDhd5NigSC{ZXzheIl3\SC2bzDjc451em:uLDDFR|UxRTNwNTFOwG0> M4LJNVI2PThzMk[x
human SKOV3 cells M3nQTWN6fG:2b4jpZ4l1gSCjc4PhfS=> NX\OeppkPCCq NI\uUWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyClbz3jeYx1fXKnZDD3bZRpKGi3bXHuJI1me2:2aHXsbYFtKGOnbHzzJIFnfGW{IESgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG|ZTDhd5NigSC{ZXzheIl3\SC2bzDjc451em:uLDDFR|UxRTVizszN MX2yOVU5OTJ4MR?=
human SU86 cells NEjZdGFEgXSxdH;4bYNqfHliYYPzZZk> NUXuUJVvPCCq M1[4cWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNWQDZiY3XscJMh[29vY4XseJVz\WRid3n0bEBpfW2jbjDt[ZNwfGinbHnhcEBk\WyuczDh[pRmeiB2IHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2ViYYPzZZkhemWuYYTpeoUhfG9iY3;ueJJwdCxiRVO1NF02NjlizszN MYSyOVU5OTJ4MR?=
human HT-29 cells NYXQXYQ{TnWwY4Tpc44h[XO|YYm= NV;aWWRPOzBibXnudy=> NUPHNmFiWmWmdXP0bY9vKG:oIHPlcIx2dGG{IFfTTEBt\X[nbDDpckBpfW2jbjDIWE0zQSClZXzsd{BqdmO3YnH0[YQh\m:{IEOwJI1qdnNiZn;scI94\WRiYomgZ48u[3WudIXybY5oKHerdHigbJVu[W5ibXXzc5Rp\WyrYXygZ4VtdHNibXXhd5Vz\WRiYX\0[ZIhPSCqcoOgZpkh\mm{ZX\sfUBtfWOrZnXyZZNmKGG|c3H5 NEHISoozPTV6MUK2NS=>

... Click to View More Cell Line Experimental Data

In vivo BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation. [2] BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. [3]


Kinase Assay:[1]
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In Gel Kinase Assay:

In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.
Cell Research:[1]
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  • Cell lines: HUVEC cells
  • Concentrations: ~20 μM
  • Incubation Time: 16 hours
  • Method: Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Rat model of pneumococcal meningitis
  • Dosages: PBS
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (200.53 mM)
Water Insoluble
Ethanol '50 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 249.33


CAS No. 196309-76-9
Storage powder
in solvent
Synonyms Bay 11-7083
Smiles CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)C=CC#N

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IκB/IKK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID