Bay 11-7085

For research use only.

Catalog No.S7352 Synonyms: Bay 11-7083

31 publications

Bay 11-7085 Chemical Structure

CAS No. 196309-76-9

BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.

Selleck's Bay 11-7085 has been cited by 31 publications

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Biological Activity

Description BAY 11-7085 (Bay 11-7083) is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Features Selective IκBα phosphorylation inhibitor.
IκBα phosphorylation [1]
10 μM
In vitro

BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells. [1] BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel. [3] Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NGqycZlHfW6ldHnvckBie3OjeR?= MYrJcohq[mm2aX;uJI9nKEmOLUGtZYxxcGFvaX7keYNm\CCQRj3rZZBx[UJiYXP0bZZifGmxbjDpckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IHLsc4NscW6pIH;mJJA2OC:yNkWgcpVkdGWjcjD0doFve2yxY3H0bY9vNCCLQ{WwQVAvOSEQvF2= MXOxO|g1PTh3MB?=
human A2780 cells NViy[pBzTnWwY4Tpc44h[XO|YYm= MVTBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDMke4NEBk\WyuczDjc{1kfWy2dYLl[EB4cXSqIH3ld491cGWuaXHsJINmdGxibX;uc4xigWW{czDifUBncXKnZnz5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0zNjZizszN NFL4O3UzOjN{NkO5OS=>
human BxPC3 cells Mln1R5l1d3SxeHnjbZR6KGG|c3H5 NWrrbpVyPCCq MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCfHBEOyClZXzsd{Bkdy2ldXz0eZJm\CC5aYToJIh2dWGwIH3ld491cGWuaXHsJINmdGy|IHHmeIVzKDRiaILzJIJ6KG[rcnXmcJkhdHWlaX\ldoF{\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCHQ{WwQVMvPSEQvF2= MlrhNlU2QDF{NkG=
human SKOV3 cells MUDDfZRwfG:6aXPpeJkh[XO|YYm= NVnWNGdrPCCq MVPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{Bkdy2ldXz0eZJm\CC5aYToJIh2dWGwIH3ld491cGWuaXHsJINmdGy|IHHmeIVzKDRiaILzJIJ6KG[rcnXmcJkhdHWlaX\ldoF{\SCjc4PhfUBz\WyjdHn2[UB1dyClb370do9tNCCHQ{WwQVUh|ryP MWOyOVU5OTJ4MR?=
human SU86 cells M2TpNmN6fG:2b4jpZ4l1gSCjc4PhfS=> NELBNXY1KGh? MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTWVg3KGOnbHzzJINwNWO3bIT1doVlKHerdHigbJVu[W5ibXXzc5Rp\WyrYXygZ4VtdHNiYX\0[ZIhPCCqcoOgZpkh\mm{ZX\sfUBtfWOrZnXyZZNmKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4wtKEWFNUC9OU46KM7:TR?= MoHWNlU2QDF{NkG=
human HT-29 cells MofDSpVv[3Srb36gZZN{[Xl? NHjt[|A{OCCvaX7z NGjPfHZT\WS3Y4Tpc44hd2ZiY3XscJVt[XJiR2PIJIxmfmWuIHnuJIh2dWGwIFjUMVI6KGOnbHzzJIlv[3WkYYTl[EBnd3JiM{CgcYlveyCob3zsc5dm\CCkeTDjc{1kfWy2dYLpcochf2m2aDDoeY1idiCvZYPveIhmdGmjbDDj[YxteyCvZXHzeZJm\CCjZoTldkA2KGi{czDifUBncXKnZnz5JIx2[2moZYLhd4Uh[XO|YYm= Mm[3NlU2QDF{NkG=

... Click to View More Cell Line Experimental Data

In vivo BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation. [2] BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. [3]


Kinase Assay:[1]
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In Gel Kinase Assay:

In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.
Cell Research:[1]
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  • Cell lines: HUVEC cells
  • Concentrations: ~20 μM
  • Incubation Time: 16 hours
  • Method: Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Rat model of pneumococcal meningitis
  • Dosages: PBS
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (200.53 mM)
Ethanol 50 mg/mL (200.53 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 249.33


CAS No. 196309-76-9
Storage powder
in solvent
Synonyms Bay 11-7083
Smiles CC(C)(C)C1=CC=C(C=C1)S(=O)(=O)C=CC#N

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IκB/IKK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID