Catalog No.S7352 Synonyms: Bay 11-7083
Molecular Weight(MW): 249.33
BAY 11-7085 is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Cited by 17 Publications
3 Customer Reviews
VSMCs were pretreated with BAY 11-7085 (BAY, 10μmol/L) for 1h and further treated with or without 10-7mol/L salusin-β for 30min. A, Cytoplasmic extracts (Cy) were obtained and the phosphorylated IκBα, IκBα and HDCA3 protein levels were measured by western blot analysis. B, nuclear extracts (Nu) were obtained and the nuclear p50, p65 and HDAC3 protein levels were measured by western blot analysis. Data are expressed as means ± SD (n=3). *P< 0.05 versus the control with no treatment; #P< 0.05 versus 10-7mol/L salusin-β treatment alone.
Cell Physiol Biochem, 2015, 36(6):2466-79. Bay 11-7085 purchased from Selleck.
A549 cells were pre-treated with Bay 11e7085 or LY2409881 for 2 h, followed by transfection with Angptl2 siRNA (si-2) and/or pGMNF-kB-GFP for 48 h. Then, all cells were incubated with PQ for an additional 24 h. RT-qPCR analysis of (D) IL-1b, TNF-a and IL-6 in cells.
Biochem Biophys Res Commun, 2018, 503(1):94-101. Bay 11-7085 purchased from Selleck.
A) Histograms represent mean±SEM of ET-1 mRNA expression. CLL cells treated with BAY 11- 7085 10 μM show less ET-1 level in comparison with controls (indicated as 100%) (*p≤0.05, n=5, Wilcoxon test). B) Histograms represent MFIR relative to ET-1 protein measured by flow cytometry in CLL samples treated or not (control) with BAY 11-7085 10μM (*p≤0.05, n=8, Wilcoxon test). In the right panel, the flow-cytometry peaks represent the FITC fluorescence intensity relative to ET-1 protein or isotype, measured in a representative CLL sample, in presence or not of BAY 11-7085 10 μM: the NF-kB inhibitor strongly reduces ET-1 MFI in comparison to control whereas the MFI of isotype remains mostly unchanged. C) ET-1 immunofluorescence of a representative CLL sample confirms a lower intensity of ET-1 protein in presence of BAY 11-7085 10 μM, if compared to control. ET-1 protein is stained in green colour whereas cell nuclei are in blue colour.
Leuk Res, 2017, 54:17-24. Bay 11-7085 purchased from Selleck.
Purity & Quality Control
Choose Selective IκB/IKK Inhibitors
|Description||BAY 11-7085 is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.|
|Features||Selective IκBα phosphorylation inhibitor.|
BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells.  BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel.  Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. 
|In vivo||BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation.  BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. |
In Gel Kinase Assay:In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.
|In vitro||DMSO||50 mg/mL (200.53 mM)|
|Ethanol||50 mg/mL (200.53 mM)|
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