Bay 11-7085

Catalog No.S7352 Synonyms: Bay 11-7083

Bay 11-7085 Chemical Structure

Molecular Weight(MW): 249.33

BAY 11-7085 is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.

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Cited by 7 Publications

3 Customer Reviews

  • VSMCs were pretreated with BAY 11-7085 (BAY, 10μmol/L) for 1h and further treated with or without 10-7mol/L salusin-β for 30min. A, Cytoplasmic extracts (Cy) were obtained and the phosphorylated IκBα, IκBα and HDCA3 protein levels were measured by western blot analysis. B, nuclear extracts (Nu) were obtained and the nuclear p50, p65 and HDAC3 protein levels were measured by western blot analysis. Data are expressed as means ± SD (n=3). *P< 0.05 versus the control with no treatment; #P< 0.05 versus 10-7mol/L salusin-β treatment alone.

    Cell Physiol Biochem, 2015, 36(6):2466-79. Bay 11-7085 purchased from Selleck.

    A549 cells were pre-treated with Bay 11e7085 or LY2409881 for 2 h, followed by transfection with Angptl2 siRNA (si-2) and/or pGMNF-kB-GFP for 48 h. Then, all cells were incubated with PQ for an additional 24 h. RT-qPCR analysis of (D) IL-1b, TNF-a and IL-6 in cells.

    Biochem Biophys Res Commun, 2018, 503(1):94-101. Bay 11-7085 purchased from Selleck.

  • A) Histograms represent mean±SEM of ET-1 mRNA expression. CLL cells treated with BAY 11- 7085 10 μM show less ET-1 level in comparison with controls (indicated as 100%) (*p≤0.05, n=5, Wilcoxon test). B) Histograms represent MFIR relative to ET-1 protein measured by flow cytometry in CLL samples treated or not (control) with BAY 11-7085 10μM (*p≤0.05, n=8, Wilcoxon test). In the right panel, the flow-cytometry peaks represent the FITC fluorescence intensity relative to ET-1 protein or isotype, measured in a representative CLL sample, in presence or not of BAY 11-7085 10 μM: the NF-kB inhibitor strongly reduces ET-1 MFI in comparison to control whereas the MFI of isotype remains mostly unchanged. C) ET-1 immunofluorescence of a representative CLL sample confirms a lower intensity of ET-1 protein in presence of BAY 11-7085 10 μM, if compared to control. ET-1 protein is stained in green colour whereas cell nuclei are in blue colour.

    Leuk Res, 2017, 54:17-24. Bay 11-7085 purchased from Selleck.

Purity & Quality Control

Choose Selective IκB/IKK Inhibitors

Biological Activity

Description BAY 11-7085 is an irreversible inhibitor of TNFα-induced IκBα phosphorylation with IC50 of 10 μM.
Features Selective IκBα phosphorylation inhibitor.
Targets
IκBα phosphorylation [1]
10 μM
In vitro

BAY 11-7085 inhibits TNFα-induced expression of adhesion molecules E-selectin, VCAM-1, and ICAM-1 by inhibition of NF-κB without detectable cytotoxicity at 10 μM in HUVEC cells. [1] BAY 11-7085 enhances the inhibition of NFkappaB activity by paclitaxel and decreases the viability of cells treated with paclitaxel. [3] Besides, combination of Bay11-7085 and LY294002 leads to synergistic apoptotic effect in PEL cells. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells MVPGeY5kfGmxbjDhd5NigQ>? M2Ls[mlvcGmkaYTpc44hd2ZiSVytNU1idHCqYT3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgZoxw[2urbnegc4YheDVyL4C2OUBvfWOuZXHyJJRz[W6|bH;jZZRqd25uIFnDOVA:OC5zIN88US=> MVqxO|g1PTh3MB?=
human A2780 cells MYPGeY5kfGmxbjDhd5NigQ>? NEP3fnpCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bkdy2ldXz0eZJm\CC5aYToJI1me2:2aHXsbYFtKGOnbHygcY9vd2yjeXXyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1{Lk[g{txO NVnuWo5bOjJ|Mk[zPVU>
human BxPC3 cells MmW5R5l1d3SxeHnjbZR6KGG|c3H5 MXO0JIg> MmDNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRphRSzNiY3XscJMh[29vY4XseJVz\WRid3n0bEBpfW2jbjDt[ZNwfGinbHnhcEBk\WyuczDh[pRmeiB2IHjyd{BjgSCoaYLl[ox6KGy3Y3nm[ZJie2ViYYPzZZkhemWuYYTpeoUhfG9iY3;ueJJwdCxiRVO1NF0{NjVizszN MoXNNlU2QDF{NkG=
human SKOV3 cells NH3nfo9EgXSxdH;4bYNqfHliYYPzZZk> NILjcWM1KGh? NH7ZNGVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyClbz3jeYx1fXKnZDD3bZRpKGi3bXHuJI1me2:2aHXsbYFtKGOnbHzzJIFnfGW{IESgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG|ZTDhd5NigSC{ZXzheIl3\SC2bzDjc451em:uLDDFR|UxRTVizszN NViwVooyOjV3OEGyOlE>
human SU86 cells MojKR5l1d3SxeHnjbZR6KGG|c3H5 MkjSOEBp MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTWVg3KGOnbHzzJINwNWO3bIT1doVlKHerdHigbJVu[W5ibXXzc5Rp\WyrYXygZ4VtdHNiYX\0[ZIhPCCqcoOgZpkh\mm{ZX\sfUBtfWOrZnXyZZNmKGG|c3H5JJJmdGG2aY\lJJRwKGOxboTyc4wtKEWFNUC9OU46KM7:TR?= MUOyOVU5OTJ4MR?=
human HT-29 cells NYnleZRETnWwY4Tpc44h[XO|YYm= MlfjN|AhdWmwcx?= MkfjVoVlfWO2aX;uJI9nKGOnbHz1cIFzKEeVSDDs[ZZmdCCrbjDoeY1idiCKVD2yPUBk\WyuczDpcoN2[mG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliY3:tZ5VtfHW{aX7nJJdqfGhiaIXtZY4hdWW|b4To[Yxq[WxiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgOUBpenNiYomg[olz\W[ueTDseYNq\mW{YYPlJIF{e2G7 MoDmNlU2QDF{NkG=

... Click to View More Cell Line Experimental Data

In vivo BAY 11-7085 inhibits the meningitis-associated increase in NF-κB activity, and results in an improvement of the clinical status of infected rats and a marked attenuation of meningitis-associated CNS complications and meningeal inflammation. [2] BAY 11-7085 increases the efficacy of paclitaxel-induced inhibition of intraabdominal dissemination and production of ascites in athymic nude mice bearing Caov-3 cells. [3]

Protocol

Kinase Assay:[1]
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In Gel Kinase Assay:

In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30 °C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control.
Cell Research:[1]
+ Expand
  • Cell lines: HUVEC cells
  • Concentrations: ~20 μM
  • Incubation Time: 16 hours
  • Method: Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: Rat model of pneumococcal meningitis
  • Formulation: 20 mg
  • Dosages: PBS
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (200.53 mM)
Ethanol 50 mg/mL (200.53 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 249.33
Formula

C13H15NO2S

CAS No. 196309-76-9
Storage powder
in solvent
Synonyms Bay 11-7083

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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IκB/IKK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID