Topotecan HCl

For research use only.

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

18 publications

Topotecan HCl Chemical Structure

CAS No. 119413-54-6

Topotecan HCl (NSC609699, Nogitecan, SKFS 104864A) is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively. Topotecan HCl induces autophagy and apoptosis.

Size Price Stock Quantity  
10mM (1mL in DMSO) EUR 226 In stock
EUR 176 In stock
EUR 314 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Topotecan HCl has been cited by 18 publications

2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

  • Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl (NSC609699, Nogitecan, SKFS 104864A) is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively. Topotecan HCl induces autophagy and apoptosis.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NFvyWnJEgXSxdH;4bYPDqGG|c3H5 MmLuO|IhcA>? MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIHnjOVA:OSCwTR?= NYXrSnVrOjB|N{GxPFM>
human Bel7402 cells NV6zPYFsS3m2b4TvfIlkyqCjc4PhfS=> Mn\wPVYhcA>? NGX1flNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NECyJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5{IH7N NGHPcoIyPTF4NUG0OC=>
human HCT8 cells MUnDfZRwfG:6aXRCpIF{e2G7 MWK5OkBp NXr2RmlzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEvPSCwTR?= Mo\nNVUyPjVzNES=
human MCF7 cells M{ThU2N6fG:2b4jpZ:Kh[XO|YYm= MorTPVYhcA>? NGfkTYhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU45KG6P MnLzNVUyPjVzNES=
human A549 cells MYTDfZRwfG:6aXRCpIF{e2G7 NED1OXA6PiCq Mm\yR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUOuNkBvVQ>? MVKxOVE3PTF2NB?=
human colon carcinoma SW620 cell MmToR5l1d3SxeHnjxsBie3OjeR?= MUDDfZRwfG:6aXOgdI91\W62aXH0bY9vKG:oIGTvdI91\WOjbjCoWHAqKGK7IITo[UBkd22yb4Xu[EBqdiCqdX3hckBkd2yxbjDjZZJkcW6xbXGgV3c3OjBiY3XscEBtcW6nLDDJR|UxRTNwMjDuUS=> NHixe5MyOjRyOEewOy=>
human MESSA cells NHPuOZNEgXSxdH;4bYPDqGG|c3H5 NF73[Wk4OiCq MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXNUSSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;NDDuUS=> NFXifI4zOTN2MU[3OC=>
human BGC823 cells M3\5[WN6fG:2b4jpZ:Kh[XO|YYm= MYm5OkBp NUPJRZBoS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkeFOEKzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PC5|IH7N Mln3NVUyPjVzNES=
human Ketr3 cells NYj2OW41S3m2b4TvfIlkyqCjc4PhfS=> MnjUPVYhcA>? NITxVFBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBM\XS{MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQvQSCwTR?= NG\wU4UyPTF4NUG0OC=>
LOX cells NY\QbHNbS3m2b4TvfIlkyqCjc4PhfS=> NVrjSodMS3m2b4TvfIlkcXS7IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwKGmwIFzPXEBk\WyuczCocYVt[W6xbXGpJI9nKGi3bXHuJJR2dW:{IHPlcIwhdGmwZYOgZpkhfXOrbnegUXRVKGG|c3H5MEBKSzVyPUWgcm0> M4\XNlc5PTN|M{G=
human A549 cells NEXn[oNHfW6ldHnvckBie3OjeR?= NUe3U5BRPCCq MlrORY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PSCwTR?= M1zMZlE5OjB5N{S4
human H69 cells NWP5UYVVS3m2b4TvfIlkyqCjc4PhfS=> NFK3fFU4OiCq MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTZibl2= NIn1UG0zOTN2MU[3OC=>
human A2780 cells NVvEXplmS3m2b4TvfIlkyqCjc4PhfS=> Ml\KO|IhcA>? MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDhcJBp[TWkZYThN{BqdnSnZ4LpckBie3Onc4Pl[EBieyClZXzsJJN2en[rdnHsJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;NjDuUS=> NVLRcVF7OjJ7NUmyOFY>
human LNCAP cells NGrpTY1EgXSxdH;4bYPDqGG|c3H5 NF3yU5c4OiCq M{n5fmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME25JI5O MknzNlE{PDF4N{S=
human HOP62 cells NILUVY5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWTQbZpUT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUE:SNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N MYOyOVkxQTJ5OR?=
human MCF7 cells Mk\US5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1:1RWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCLQ{WwQVExKG6P M3nRO|I2QTB7Mke5
human UACC62 cells MlXVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGGzWXVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBWSUOFNkKgZ4VtdHNiYomg[ol3\S2mb4PlJIdzd3e2aDDpcohq[mm2aX;uJIF{e2G7LDDJR|UxRTFyIH7N NYPTN|Z5OjV7MEmyO|k>
human DU145 cells MljCS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn73S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[nliZnn2[U1ld3OnIHfyc5d1cCCrbnjpZol1cW:wIHHzd4F6NCCLQ{WwQVExKG6P M{X2WlI2QTB7Mke5
LoVo cell line NUO1[4dvS3m2b4TvfIlkyqCjc4PhfS=> M3Tkc2N6fG:2b4jpZ{Bxd3SnboTpZZRqd25ib3[gWI9xd3SnY3HuJEhVWCliYomgeIhmKGOxbYDveY5lKGmwIDjoeY1idiClb3zvdoVkfGGuIHPhcoNmeiCOb2\vJINmdGxibHnu[UwhUUN3ME2xNU43KG6P NYLaXJUyOTJ2MEi3NFc>
human lymphoblast tumor cell line RPM18402 NFTRSI1EgXSxdH;4bYPDqGG|c3H5 M4PT[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIx6dXCqb3LsZZN1KHS3bX;yJINmdGxibHnu[UBTWE1zOESwNkwhUUN3ME2xNkBvVQ>? MlvsNVI{QTJ5NEW=
human HL60 cells MkHSSpVv[3Srb36gZZN{[Xl? MYXBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHL5JHNTSiCvZYToc4QtKEmFNUC9NVIhdk1? NXfTT4x{OTl3NEG0PFM>
human A375 cells MX7DfZRwfG:6aXRCpIF{e2G7 MVe3NkBp Mo\PR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVM4PSClZXzsd{Bi\nSncjC3NkBpenNiYomgZYxidWG{IHLseYUh[XO|YYmsJGlEPTB;MUOgcm0> NHPBSHIzOTN2MU[3OC=>
COR-L23/P cell M2TmeWN6fG:2b4jpZ:Kh[XO|YYm= NEjveVZEgXSxdH;4bYNqfHlibXXhd5Vz\WRidYPpcochfGinIFPPVk1NOjNicHHy[Y51[WxiKFPPVk1NOjNxUDmgbJVu[W5ibn;uJJNu[WyuIHPlcIwhdHWwZzDjZZJkcW6xbXGgZ4VtdCCuaX7lMEBKSzVyPUGzMlIhdk1? NIC3c5AyOThyNkeyOC=>
human NCI-H460 cells NXnqfmM{WHKxbHnm[ZJifGmxbjDhd5NigQ>? NV;zeoFDPzJiaB?= MlrXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFQ3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgZ492dHSncjDjc5VvfGW{IHHuZYx6e2m|LDDJR|UxRTF4IH7N NHGyb5MzOTd6M{O2PS=>
human MOLT4 cells M2jBbmN6fG:2b4jpZ:Kh[XO|YYm= MmXjO|IhcA>? M135b2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1QVFR2IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkhdk1? MkTiNlQ6ODB5MkW=
human LoVo cells NX3LeGFvS3m2b4TvfIlkyqCjc4PhfS=> MkjsO|IhcA>? MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCgcm0> Ml74NlA{PzFzOEO=
RPM18402 tumor cell line Moi0VJJwdGmoZYLheIlwdiCjc4PhfS=> NHLzNVVEd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgZ4VtdCCycn;sbYZmemG2aX;uJIlvKFKSTUG4OFAzKHS3bX;yJINmdGxibHnu[UwhUUN3ME2yNEBvVQ>? NGfpSogyPTR6MkmyPS=>
human SN12C cells NYnRVFZET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MY\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTUlEzSyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEeLNUC9NlAhdk1? NFHtZZgzPTlyOUK3PS=>
human RPMI8226 cells NFvFR2ZEgXSxdH;4bYPDqGG|c3H5 MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSVG1KQDJ{NjDj[YxteyCkeTDNWHQhdWW2aH;kMEBKSzVyPUKxJI5O NHPFNJcyQTBzMkm5Oi=>
HT-29 cells NX3zcY1XS3m2b4TvfIlkyqCjc4PhfS=> NWTDXmJOS3m2b4TvfIlkcXS7IIfhd{Bl\XSncn3pcoVlKGmwII\peJJwKGmwIFjUMVI6KGOnbHzzLINwdG:wKTDv[kBpfW2jbjD0eY1weiClZXzsJIxqdmW|IHL5JJV{cW6pIF3UWEBie3OjeTygTWM2OD1{NTDuUS=> M3\iR|c5PTN|M{G=
SK-BR-3 cells MnzCR5l1d3SxeHnjxsBie3OjeR?= MUjJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\SBqU1utRnIuOyluIFnDOVA:OjZibl2= Mn7BNVE{OzR3Nkm=
HeLa cells NFrJVZBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1XCWWdzd3e2aDDpcohq[mm2aX;uJI9nKEinTHGgZ4VtdHNiYX\0[ZIhPCCmYYnzMEBIUTVyPUOwJI5O NGLCZnoyPzRzOEW4Ni=>
UACC 62 cells NHv3VHdEgXSxdH;4bYPDqGG|c3H5 NHuzVVNV\XO2ZXSgbY4hfmm2cn:g[o9zKGO7dH;0c5hq[2m2eTDh[4FqdnO2IHj1cYFvKHS3bX;yJINmdGxibHnu[UBWSUOFIE[yJEhu\Wyjbn;tZUktKEeLNUC9N|Ahdk1? MmPjNVA4OTR3MEK=
human HCT116 cells NH\mPWpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPsR4VYT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHL5JIZqfmVvZH;z[UBoem:5dHigbY5pcWKrdHnvckBie3OjeTygS2k2OD1|MDDuUS=> M4Dr[FI2QTB7Mke5
human lung cancer cell line (H128) MVLDfZRwfG:6aXRCpIF{e2G7 MWfJckBXcXS{bzDjfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDseY5oKGOjbnPldkBk\WyuIHzpcoUhMEhzMkipMEBKSzVyPUOxJI5O Mln2NVE{OzR3Nkm=
human MES-SA/Dx5 cells MYTDfZRwfG:6aXRCpIF{e2G7 MUDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXMuW0FxRIi1JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKE2GUkGgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTN|IH7N NF\2d2kzOTN2MU[3OC=>
human A2780 cells MVrDfZRwfG:6aXRCpIF{e2G7 MkG2O|IhcA>? NGDPSpdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM{KG6P MXmyOFkxODd{NR?=
human MDA-MB-435 cells NGToTI1EgXSxdH;4bYPDqGG|c3H5 MmjMO|IhcA>? NVjjTld5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGdKPTB;M{Sgcm0> NVe5TYpJOjJ6NkewNVk>
human stomach cancer cell line (MKN45) NFf4NnFEgXSxdH;4bYPDqGG|c3H5 MoOwTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gd5RwdWGlaDDjZY5k\XJiY3XscEBtcW6nIDjNT241PSluIFnDOVA:Ozhibl2= MVWxNVM{PDV4OR?=
human KB3-1 cells NVy4bWxXS3m2b4TvfIlkyqCjc4PhfS=> NUS5dFVWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0J|LUGgZ4VtdHNiYX\0[ZIhVVSWIHHzd4F6NCCLQ{WwQVQxKG6P NXK1WHp[OTh6MkmzN|Q>
mouse P388 cells M17kWGN6fG:2b4jpZ:Kh[XO|YYm= MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IF3UWEBie3OjeTygTWM2OD12NTDuUS=> M2TKUlE5QDJ7M{O0
A427 human lung carcinoma MXXQdo9tcW[ncnH0bY9vKGG|c3H5 MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDt[YF{fXKnZDDh[4FqdnO2IFG0NlchcHWvYX6gcJVv\yClYYLjbY5wdWFuIFnDOVA:PDlibl2= MV65PFc3OTFz
human U251 cells NETtVWRHfW6ldHnvckBie3OjeR?= NWr3XHVCPi1{NDDo M2LJe2lvcGmkaYTpc44hd2ZidH;wc4l{d22ncnHz[U0yKGmwIHj1cYFvKFV{NUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCqeYDvfIliNWmwZIXj[YQhUEmILUHhcJBp[SCjY3P1cZVt[XSrb36gbY4hdnWlbHXhdkBmgHS{YXP0JIFnfGW{IE[geI8hOjRiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXNuIFXDOVA:PTRibl2= M3K5ZlIzOzB3NkGy
human colon cancer cell line (WiDr) NGDKSVBEgXSxdH;4bYPDqGG|c3H5 NXHtdZprUW5iVnn0do8h[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4h[2:ub36gZ4Fv[2W{IHPlcIwhdGmwZTCoW4lFeiluIFnDOVA:PTZibl2= MV6xNVM{PDV4OR?=
human HepG2 cells NELNXmJEgXSxdH;4bYPDqGG|c3H5 MX[0JIRigXN? MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0JIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPU[wJI5O M1npRlE5PTV2OUC2
human KB cells NVXEdHhVS3m2b4TvfIlkyqCjc4PhfS=> MlKzO|IhcA>? MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLRkBk\WyuczDh[pRmeiB5MjDodpMhe3WuZn;ybI9l[W2rbnWgRkBkd2yxcnnt[ZRzcWNiYYPzZZktKEmFNUC9OlIvPSCwTR?= MofHNlUxODh2NU[=
human HCT8 cells Ml;lR5l1d3SxeHnjxsBie3OjeR?= NVrLeIJZOyCmYYnz NUjVeYpnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWODDj[YxteyCjZoTldkA{KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVcxKG6P M{eye|IxOzl{NUS1
human GBM2 cells MXHQdo9tcW[ncnH0bY9vKGG|c3H5 NX3LbpNMPzJiaB?= M2i5UmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvOTNizszN NITPfoUzPjN3NUWzNi=>
SK-MEL-2 cells NFXk[VREgXSxdH;4bYPDqGG|c3H5 M1HTRVczKGh? MoTWR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOLUKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTRizszN NEjpT|czOTN2MU[3OC=>
human IGROV1 cells M3nzdmN6fG:2b4jpZ:Kh[XO|YYm= MnH4O|IhcA>? NFvyNVdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBKT1KRVkGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVAvOTZizszN NX21NotuOjF|NEG2O|Q>
human U87 cells MVPQdo9tcW[ncnH0bY9vKGG|c3H5 MXu0PEBp MlnmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXOEegZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkG2JO69VQ>? M{P4e|I1QDJ4OEG4
human HT1080 cells M1fac3Bzd2yrZnXyZZRqd25iYYPzZZk> MkjnOFghcA>? M1:4SGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGSxNFgxKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFnDOVA:OC5zODFOwG0> MYOyOlQxQDhzNR?=
human Hep3B cells Mof5VJJwdGmoZYLheIlwdiCjc4PhfS=> M2\5[VczKGh? NG[xPHFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldFNDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDYWHQh[XO|YYmsJGdKPTB;MD6yNkDPxE1? NXOxT|BLOTl5OU[5OVY>
human GBM3 cells NHTmVm9Rem:uaX\ldoF1cW:wIHHzd4F6 MWK3NkBp MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEeETUOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiN{KgbJJ{KGK7IGfTWE0yKG2ndHjv[EwhUUN3ME2wMlM5KM7:TR?= NX7jWVFzOjZ|NUW1N|I>
human KBVIN cells MmWzR5l1d3SxeHnjxsBie3OjeR?= MnixO|IhcA>? NH;SSlBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSl[LTjDj[YxteyCjZoTldkA4OiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVAvPCEQvF2= M3\udVI2PDN6N{[5
human GBM1 cells MVHQdo9tcW[ncnH0bY9vKGG|c3H5 MkHNO|IhcA>? NV3ieo9jSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0yKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:OC52NjFOwG0> M13NeFI3OzV3NUOy
human U937 cells MnjzR5l1d3SxeHnjxsBie3OjeR?= NYjNNnd6PDhiaB?= NFzkOI1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{KGW6cILld5NqdmdicEWzJI12fGGwdDDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7IP-8kEBKSzVyPUGuNUDPxE1? M13NbVE6PzF3M{G5

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LC3 / p62 ; 

PubMed: 23024792     


The indicated cells were treated with the indicated concentrations of topotecan for 24 h. The lysates were analysed by immunoblotting with The LC3 and P62 antibodies.

p53 / sesn2 / p-AMPK ; 

PubMed: 23024792     


HCT116 and LS-174T cells were treated with various concentrations of TPT for 24 h. The levels of P53, sestrin2 and p-AMPK were analysed by immunoblotting. 

23024792
Growth inhibition assay
Cell viability ; 

PubMed: 28544814     


Dose-response curves for topotecan (panel A) were determined in neuroblastoma cell lines The IC50 for the drugs are indicated. Changes in LAN-1 cell viablity as a function of different topotecan exposure times (panel B) were determined.

28544814
In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
in solvent
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A
Smiles CCC1(C2=C(COC1=O)C(=O)N3CC4=CC5=C(C=CC(=C5CN(C)C)O)N=C4C3=C2)O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04455139 Not yet recruiting Drug: Topotecan|Drug: Melphalan Eye Cancer Retinoblastoma Prof. Beck Popovic Maja|University of Lausanne Hospitals August 1 2020 Phase 2
NCT04428879 Recruiting Drug: Topotecan Episcleral Plaque Retinoblastoma The Hospital for Sick Children June 15 2020 Phase 1
NCT04156347 Not yet recruiting Drug: Episcleral Topotecan Retinoblastoma Targeted Therapy Technologies LLC April 1 2020 Phase 1
NCT04022876 Recruiting Drug: ALRN-6924|Drug: Topotecan Small-cell Lung Cancer Aileron Therapeutics September 3 2019 Phase 1|Phase 2
NCT03927274 Recruiting Drug: Topotecan Glioma of Brain H. Lee Moffitt Cancer Center and Research Institute June 20 2019 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products

Tags: buy Topotecan HCl | Topotecan HCl supplier | purchase Topotecan HCl | Topotecan HCl cost | Topotecan HCl manufacturer | order Topotecan HCl | Topotecan HCl distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID