Topotecan HCl

For research use only.

Catalog No.S1231 Synonyms: NSC609699, Nogitecan HCl, SKFS 104864A

18 publications

Topotecan HCl Chemical Structure

Molecular Weight(MW): 457.91

Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.

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10mM (1mL in DMSO) USD 230 In stock
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Selleck's Topotecan HCl has been cited by 18 publications

2 Customer Reviews

  • Cells were treated at the same concentrations as in Caspase3/7 assay for 16 hours and total cell lysates were prepared for Western blotting. GAPDH was used as a loading control.

    BMC Cancer, 2015, 10.1186/s12885-015-1231-z. Topotecan HCl purchased from Selleck.

  • Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Pax3:Foxo1a knockdown reduced the concentration at which viability was impaired by 50% (IC50) of topotecan by 4.8 fold, respectively, yet did not affect the IC50 of mafosfamide.

    PLoS Genet 2014 10(1), e1004107. Topotecan HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Topotecan HCl is a topoisomerase I inhibitor for MCF-7 Luc cells and DU-145 Luc cells with IC50 of 13 nM and 2 nM in cell-free assays, respectively.
Features Topotecan is a water-soluble derivative of camptothecin.
Targets
Topo I (DU-145 Luc cells) [1]
(Cell-free assay)
Topo I (MCF-7 Luc cells) [1]
(Cell-free assay)
2 nM 13 nM
In vitro

Stronger drug activity of Topotecan is observed for DU-145 Luc and MCF-7 Luc cells. [1] Topotecan causes cytotoxicity during the course of DNA replication by stabilizing the covalent complex between topoisomerase I and DNA and preventing the religation of enzyme-linked single-strand DNA break. Topotecan stabilizes topoisomerase I/DNA cleavable complexes in radiation-resistant human B-lineage acute lymphoblastic leukemia (ALL) cells, causes rapid apoptotic cell death despite high-level expression of bcl-2 protein, and inhibits ALL cell clonogenic growth in a dose-dependent fashion. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells MnXHR5l1d3SxeHnjxsBie3OjeR?= NIHTNmc4OiCq NUezeIRqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBq[zVyPUGgcm0> MmDxNlA{PzFzOEO=
human Bel7402 cells M17RZWN6fG:2b4jpZ:Kh[XO|YYm= NXLYdokyQTZiaB?= MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU4zKG6P MknXNVUyPjVzNES=
human HCT8 cells NF3wN5dEgXSxdH;4bYPDqGG|c3H5 MmHuPVYhcA>? MoLSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVQCClZXzsd{Bi\nSncjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOUBvVQ>? MXGxOVE3PTF2NB?=
human MCF7 cells NFj3Zo9EgXSxdH;4bYPDqGG|c3H5 M1X5UVk3KGh? NHLDPYdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NU45KG6P M1H0elE2OTZ3MUS0
human A549 cells NFvwN5JEgXSxdH;4bYPDqGG|c3H5 NIrMXIg6PiCq MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mz6yJI5O M2f2UVE2OTZ3MUS0
human colon carcinoma SW620 cell M4naemN6fG:2b4jpZ:Kh[XO|YYm= NGfqeZhEgXSxdH;4bYMheG:2ZX70bYF1cW:wIH;mJHRweG:2ZXPhckApXFBrIHL5JJRp\SClb33wc5Vv\CCrbjDoeY1idiClb3zvckBk[XKlaX7vcYEhW1d4MkCgZ4VtdCCuaX7lMEBKSzVyPUOuNkBvVQ>? NF22RnMyOjRyOEewOy=>
human MESSA cells M{HKN2N6fG:2b4jpZ:Kh[XO|YYm= M2PLUVczKGh? M{jvUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1GW1ODIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME20JI5O NVfuWmFGOjF|NEG2O|Q>
human BGC823 cells MXPDfZRwfG:6aXRCpIF{e2G7 MYS5OkBp NH7IOo1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01NjNibl2= MYKxOVE3PTF2NB?=
human Ketr3 cells MnrqR5l1d3SxeHnjxsBie3OjeR?= NVe1VoROQTZiaB?= M2\DNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtmfHJ|IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE46KG6P NIO0blEyPTF4NUG0OC=>
LOX cells MkDUR5l1d3SxeHnjxsBie3OjeR?= MVjDfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hVE:[IHPlcIx{KCivZXzhco9u[Slib3[gbJVu[W5idIXtc5Ih[2WubDDsbY5meyCkeTD1d4lv\yCPVGSgZZN{[XluIFnDOVA:PSCwTR?= NUDCVFluPzh3M{OzNS=>
human A549 cells M1nkS2Z2dmO2aX;uJIF{e2G7 Ml;TOEBp NYHveYk1SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NTDuUS=> M4PKflE5OjB5N{S4
human H69 cells MWrDfZRwfG:6aXRCpIF{e2G7 MoLNO|IhcA>? NUXGWoFwS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDZ7IHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME22JI5O MUKyNVM1OTZ5NB?=
human A2780 cells NYPyemE5S3m2b4TvfIlkyqCjc4PhfS=> NECw[mE4OiCq NIXUSYREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCxdnXy[ZhxemW|c3nu[{BidHCqYUXi[ZRiOyCrboTl[5JqdiCjc4Pld5Nm\CCjczDj[YxtKHO3co\peoFtKGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUC9OkBvVQ>? NED6WFMzOjl3OUK0Oi=>
human LNCAP cells M3v3SmN6fG:2b4jpZ:Kh[XO|YYm= NXHlT4JEPzJiaB?= NXi5SJE6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVE6FQWCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCLQ{WwQVkhdk1? MlLsNlE{PDF4N{S=
human HOP62 cells NGTQOIVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NG[5S4JIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJV1B4MjDj[YxteyCkeTDmbZZmNWSxc3Wg[5Jwf3SqIHnubIljcXSrb36gZZN{[XluIFnDOVA:OTBibl2= M3;JO|I2QTB7Mke5
human MCF7 cells NEnNPFVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoHuS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWNHPyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEmFNUC9NVAhdk1? NUPafm15OjV7MEmyO|k>
human UACC62 cells NVjkTnVET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUfKXmc5T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQ{[yJINmdGy|IHL5JIZqfmVvZH;z[UBoem:5dHigbY5pcWKrdHnvckBie3OjeTygTWM2OD1zMDDuUS=> NYniZWlkOjV7MEmyO|k>
human DU145 cells MoPaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2jOTGdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGRWOTR3IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiSVO1NF0yOCCwTR?= NXLOOlY6OjV7MEmyO|k>
LoVo cell line NYPOd|BmS3m2b4TvfIlkyqCjc4PhfS=> Mn\oR5l1d3SxeHnjJJBwfGWwdHnheIlwdiCxZjDUc5BwfGWlYX6gLHRRMSCkeTD0bIUh[2:vcH;1coQhcW5iKHj1cYFvKGOxbH;y[YN1[WxiY3HuZ4VzKEyxVn:gZ4VtdCCuaX7lMEBKSzVyPUGxMlYhdk1? NYi1O5VHOTJ2MEi3NFc>
human lymphoblast tumor cell line RPM18402 M4HIdmN6fG:2b4jpZ:Kh[XO|YYm= Mk\BR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gcJlueGixYnzhd5QhfHWvb4KgZ4VtdCCuaX7lJHJRVTF6NECyMEBKSzVyPUGyJI5O M1KwdVEzOzl{N{S1
human HL60 cells M2Pa[WZ2dmO2aX;uJIF{e2G7 MVfBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTE[wJINmdGy|IHL5JHNTSiCvZYToc4QtKEmFNUC9NVIhdk1? MljaNVk2PDF2OEO=
human A375 cells NEjRRllEgXSxdH;4bYPDqGG|c3H5 MUG3NkBp NVHrVHRwS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTN5NTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEmFNUC9NVMhdk1? NV\nd|d[OjF|NEG2O|Q>
COR-L23/P cell MXTDfZRwfG:6aXRCpIF{e2G7 M4nvWWN6fG:2b4jpZ4l1gSCvZXHzeZJm\CC3c3nu[{B1cGViQ1;SMWwzOyCyYYLlcpRidCBqQ1;SMWwzOy:SKTDoeY1idiCwb36gd41idGxiY3XscEBtfW6pIHPhdoNqdm:vYTDj[YxtKGyrbnWsJGlEPTB;MUOuNkBvVQ>? NGXYcY4yOThyNkeyOC=>
human NCI-H460 cells NES0ZplRem:uaX\ldoF1cW:wIHHzd4F6 MnXVO|IhcA>? NIewbG1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTU1JPDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBkd3WudHXyJINwfW62ZYKgZY5idHm|aYOsJGlEPTB;MU[gcm0> NIPO[GwzOTd6M{O2PS=>
human MOLT4 cells M1znTWN6fG:2b4jpZ:Kh[XO|YYm= M1zieFczKGh? MnWwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW9NXDRiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPUBvVQ>? NE\mbW8zPDlyMEeyOS=>
human LoVo cells NYDmVHVFS3m2b4TvfIlkyqCjc4PhfS=> MnnwO|IhcA>? MkDVR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUI9XdyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKwJI5O Ml7XNlA{PzFzOEO=
RPM18402 tumor cell line MVvQdo9tcW[ncnH0bY9vKGG|c3H5 NWX5dlFlS2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JINmdGxicILvcIln\XKjdHnvckBqdiCUUF2xPFQxOiC2dX3vdkBk\WyuIHzpcoUtKEmFNUC9NlAhdk1? NX;FTXM2OTV2OEK5Nlk>
human SN12C cells NWW4VIFPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDTUlEzSyClZXzsd{BjgSCoaY\lMYRwe2ViZ4Lve5RpKGmwaHnibZRqd25iYYPzZZktKEeLNUC9NlAhdk1? NGPqNWIzPTlyOUK3PS=>
human RPMI8226 cells M1i5dmN6fG:2b4jpZ:Kh[XO|YYm= NH3vS2REgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTWE2LOEKyOkBk\WyuczDifUBOXFRibXX0bI9lNCCLQ{WwQVIyKG6P MVSxPVAyOjl7Nh?=
HT-29 cells NXHJOnpUS3m2b4TvfIlkyqCjc4PhfS=> MX7DfZRwfG:6aXPpeJkhf2G|IHTleIVzdWmwZXSgbY4hfmm2cn:gbY4hUFRvMkmgZ4VtdHNqY3;sc44qKG:oIHj1cYFvKHS3bX;yJINmdGxibHnu[ZMh[nlidYPpcochVVSWIHHzd4F6NCCLQ{WwQVI2KG6P M1nJcVc5PTN|M{G=
SK-BR-3 cells NFPVeFhEgXSxdH;4bYPDqGG|c3H5 Ml3mTY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmViKGPLMWJTNTNrLDDJR|UxRTJ4IH7N NVHNZYs6OTF|M{S1Olk>
HeLa cells NYG5RWdST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{nLPWdzd3e2aDDpcohq[mm2aX;uJI9nKEinTHGgZ4VtdHNiYX\0[ZIhPCCmYYnzMEBIUTVyPUOwJI5O NV;jS|BZOTd2MUi1PFI>
UACC 62 cells M4i5U2N6fG:2b4jpZ:Kh[XO|YYm= MVrU[ZN1\WRiaX6geol1em9iZn;yJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJR2dW:{IHPlcIwhdGmwZTDVRWNEKDZ{IDjt[Yxidm:vYTmsJGdKPTB;M{Cgcm0> M{nl[lExPzF2NUCy
human HCT116 cells M4H4Omdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlHuS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTGNVOTF4IHPlcIx{KGK7IH\peoUu\G:|ZTDndo94fGhiaX7obYJqfGmxbjDhd5NigSxiR1m1NF0{OCCwTR?= MmrHNlU6ODl{N{m=
human lung cancer cell line (H128) NUDp[GVnS3m2b4TvfIlkyqCjc4PhfS=> Mmj0TY4hXmm2cn:gZ5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gcJVv\yClYX7j[ZIh[2WubDDsbY5mKCiKMUK4LUwhUUN3ME2zNUBvVQ>? NX;vT3MyOTF|M{S1Olk>
human MES-SA/Dx5 cells M17udGN6fG:2b4jpZ:Kh[XO|YYm= MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSXMuW0FxRIi1JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKE2GUkGgZYZ1\XJiN{KgbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDJR|UxRTN|IH7N MVyyNVM1OTZ5NB?=
human A2780 cells MmPiR5l1d3SxeHnjxsBie3OjeR?= M3jFeFczKGh? NFfaZ3hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM{KG6P MWqyOFkxODd{NR?=
human MDA-MB-435 cells MV;DfZRwfG:6aXRCpIF{e2G7 NEe3UHE4OiCq NVP3RnBES3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BqdmO3YnH0[YQh\m:{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGdKPTB;M{Sgcm0> NFL4dJgzOjh4N{CxPS=>
human stomach cancer cell line (MKN45) NWXK[GpoS3m2b4TvfIlkyqCjc4PhfS=> M3yyO2lvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJJN1d22jY3igZ4Fv[2W{IHPlcIwhdGmwZTCoUWtPPDVrLDDJR|UxRTN6IH7N MlfrNVE{OzR3Nkm=
human KB3-1 cells MlqwR5l1d3SxeHnjxsBie3OjeR?= Mn3xR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2I{NTFiY3XscJMh[W[2ZYKgUXRVKGG|c3H5MEBKSzVyPUSwJI5O M4\P[|E5QDJ7M{O0
mouse P388 cells M3;PbGN6fG:2b4jpZ:Kh[XO|YYm= NYrZdXhmS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWDN6ODDj[YxteyCjZoTldkBOXFRiYYPzZZktKEmFNUC9OFUhdk1? MoDTNVg5Ojl|M{S=
A427 human lung carcinoma MVPQdo9tcW[ncnH0bY9vKGG|c3H5 NHnHW4tCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBu\WG|dYLl[EBi\2GrboP0JGE1OjdiaIXtZY4hdHWwZzDjZZJkcW6xbXGsJGlEPTB;NEmgcm0> NVyxb5NYQTh5NkGxNS=>
human U251 cells MWnGeY5kfGmxbjDhd5NigQ>? M3HROlYuOjRiaB?= MWXJcohq[mm2aX;uJI9nKHSxcH;pd49u\XKjc3WtNUBqdiCqdX3hckBWOjVzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcHmyb4jpZU1qdmS3Y3XkJGhKTi1zYXzwbIEh[WOldX31cIF1cW:wIHnuJI52[2ynYYKg[Zh1emGldDDh[pRmeiB4IITvJFI1KGi{czDifUBqdW23bn;icI91KGGwYXz5d4l{NCCHQ{WwQVU1KG6P M1nwWVIzOzB3NkGy
human colon cancer cell line (WiDr) MWrDfZRwfG:6aXRCpIF{e2G7 M3u0RWlvKF[rdILvJIN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJINwdG:wIHPhcoNmeiClZXzsJIxqdmViKGfpSJIqNCCLQ{WwQVU3KG6P NFHRVpMyOTN|NEW2PS=>
human HepG2 cells MkPHR5l1d3SxeHnjxsBie3OjeR?= M3\vUlQh\GG7cx?= MmTTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOEBl[Xm|IHL5JG1VXCCjc4PhfUwhUUN3ME22NEBvVQ>? NEn6cXEyQDV3NEmwOi=>
human KB cells MoqzR5l1d3SxeHnjxsBie3OjeR?= NGLEe5o4OiCq NX3VdYU5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IIP1cIZwemixZHHtbY5mKEJiY3;sc5JqdWW2cnnjJIF{e2G7LDDJR|UxRTZ{LkWgcm0> M17qSVI2ODB6NEW2
human HCT8 cells MkCwR5l1d3SxeHnjxsBie3OjeR?= NED6[GQ{KGSjeYO= MonrR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVQCClZXzsd{Bi\nSncjCzJIRigXNiYomgUXRVKGG|c3H5MEBKSzVyPUewJI5O MnvINlA{QTJ3NEW=
human GBM2 cells MnzNVJJwdGmoZYLheIlwdiCjc4PhfS=> NULITXRzPzJiaB?= NHWweXlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVIh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF0xNjF|IN88US=> M1;vb|I3OzV3NUOy
SK-MEL-2 cells Mn\sR5l1d3SxeHnjxsBie3OjeR?= M3T1TlczKGh? M{DKd2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HTD2yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NF0xNjF2IN88US=> NXvSZ25zOjF|NEG2O|Q>
human IGROV1 cells M1q4R2N6fG:2b4jpZ:Kh[XO|YYm= NEDw[Y84OiCq MlX3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTWdTV1ZzIHPlcIx{KGGodHXyJFczKGi{czDifUBidGGvYYKgZox2\SCjc4PhfUwhUUN3ME2wMlE3KM7:TR?= M2DNUVIyOzRzNke0
human U87 cells MlXpVJJwdGmoZYLheIlwdiCjc4PhfS=> M4LzN|Q5KGh? MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6NzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwMU[g{txO NIXIblkzPDh{NkixPC=>
human HT1080 cells MYjQdo9tcW[ncnH0bY9vKGG|c3H5 NFriSVc1QCCq MkOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEGwPFAh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44hcW6ldXLheIVlKG[xcjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiSVO1NF0xNjF6IN88US=> NWK2WXBJOjZ2MEi4NVU>
human Hep3B cells MVrQdo9tcW[ncnH0bY9vKGG|c3H5 M1jZclczKGh? MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincEPCJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC[VGSgZZN{[XluIFfJOVA:OC5{MjFOwG0> NXr5W2N7OTl5OU[5OVY>
human GBM3 cells M{DjcnBzd2yrZnXyZZRqd25iYYPzZZk> NGfoR284OiCq NGfEcmtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO|IhcHK|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF0xNjN6IN88US=> MnfCNlY{PTV3M{K=
human KBVIN cells M{O4W2N6fG:2b4jpZ:Kh[XO|YYm= MlTrO|IhcA>? M{HPbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDXkmQIHPlcIx{KGGodHXyJFczKGi{czDifUBUWkJiYYPzZZktKEmFNUC9NE41KM7:TR?= MUiyOVQ{QDd4OR?=
human GBM1 cells NYnGT21uWHKxbHnm[ZJifGmxbjDhd5NigQ>? M3vVSFczKGh? M1f5TGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVAvPDZizszN MWGyOlM2PTV|Mh?=
human U937 cells MnPnR5l1d3SxeHnjxsBie3OjeR?= MWS0PEBp NIHDZXNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWQTN5IHPlcIx{KGW6cILld5NqdmdicEWzJI12fGGwdDDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7IP-8kEBKSzVyPUGuNUDPxE1? NGK2WHcyQTdzNUOxPS=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LC3 / p62 ; 

PubMed: 23024792     


The indicated cells were treated with the indicated concentrations of topotecan for 24 h. The lysates were analysed by immunoblotting with The LC3 and P62 antibodies.

p53 / sesn2 / p-AMPK ; 

PubMed: 23024792     


HCT116 and LS-174T cells were treated with various concentrations of TPT for 24 h. The levels of P53, sestrin2 and p-AMPK were analysed by immunoblotting. 

23024792
Growth inhibition assay
Cell viability ; 

PubMed: 28544814     


Dose-response curves for topotecan (panel A) were determined in neuroblastoma cell lines The IC50 for the drugs are indicated. Changes in LAN-1 cell viablity as a function of different topotecan exposure times (panel B) were determined.

28544814
In vivo Animals inoculate s.c. with DU-145 Luc cells and then treated with Topotecan demonstrates significant tumor growth and regression as measured with calipers and luminescent imaging. The correlation coefficient is 0.75 for the control untreated group and 0.93 for the Topotecan-treated group. Similarly, tumor progression and regression are measurable using luminescent imaging for untreated and Topotecan-treated mice inoculated i.p. with MCF-7 Luc cells. [1] Topotecan elicited potent antileukemic activity in severe combined immune-deficiency (SCID) mouse models of human poor prognosis ALL. Topotecan markedly improved event-free survival of SCID mice challenged with otherwise fatal doses of humaln leukemia cells at systemic drug exposure levels. [2] Gliomas preferentially express TRAIL R2 and that treatment with Topotecan significantly up-regulates its expression. [3]

Protocol

Cell Research:

[1]

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  • Cell lines: MCF-7 Luc and DU-145 Luc cells
  • Concentrations: 0 μg/mL - 0.692 μg/mL
  • Incubation Time: 96 hours
  • Method:

    Topotecan is dissolved in sterile water to a stock concentration of 1 mg/mL, diluted to 6 μg/mL in cultured medium and then serially diluted 1:4 in opaque, white tissue culture-treated microplates to a final volume of 0.1 mL/well. MCF-7 Luc and DU-145 Luc cells are resuspended in 3×104 cells/mL in DMEM with high glucose containing 10% FBS and 0.5 mg/mL Geneticin; 100 μL of cells are added in each well. Plates are incubated for 4 days at 37 °C in 95% humidity/5% CO2. After incubation, 0.05 mL of 0.1 M HEPES buffer (pH 7.9) containing 50 μg/mL D-luciferin is added to each well. After incubation at room temperature for 10 minutes, the culture microplate is measured in a microplate luminometer and a molecular light imager. Results obtained with the microplate luminometer are calculated using no inhibition control wells without exogenous drug and maximum inhibition control wells containing ATP inhibitor. Results for the molecular light imager are similarly calculated using values obtained with a 5 minutes luminescent imager.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Mice with MCF-7 Luc or DU-145 Luc cells
  • Dosages: 0.25 mg/mL
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 91 mg/mL (198.72 mM)
Water 91 mg/mL (198.72 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 457.91
Formula

C23H23N3O5.HCl

CAS No. 119413-54-6
Storage powder
in solvent
Synonyms NSC609699, Nogitecan HCl, SKFS 104864A
Smiles Cl.CCC1(O)C(=O)OCC2=C1C=C3N(CC4=C3N=C5C=CC(=C(CN(C)C)C5=C4)O)C2=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04156347 Not yet recruiting Drug: Episcleral Topotecan Retinoblastoma Targeted Therapy Technologies LLC April 1 2020 Phase 1
NCT04022876 Recruiting Drug: ALRN-6924|Drug: Topotecan Small-cell Lung Cancer Aileron Therapeutics September 3 2019 Phase 1|Phase 2
NCT03927274 Recruiting Drug: Topotecan Glioma of Brain H. Lee Moffitt Cancer Center and Research Institute June 20 2019 Early Phase 1
NCT03107988 Recruiting Drug: Lorlatinib|Drug: Cyclophosphamide|Drug: Topotecan Neuroblastoma New Approaches to Neuroblastoma Therapy Consortium|Pfizer|University of Southern California|Solving Kids'' Cancer US/EU|Children''s Neuroblastoma Cancer Foundation|The Band of Parents|The Evan Foundation|Wade''s Army|Ronan Thompson Foundation|The Catherine Elizabeth Blair Memorial Foundation|Cookies for Kids'' Cancer September 1 2017 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I would like get the compound for mice intraperitoneally injection, What would you recommend to improve solubility? What could I use as solvent (%v?) that is nontoxic (unlike Methanol) and could be injected into mice intraperitoneally?

  • Answer:

    Topotecan HCl is generally prepared by Saline for I.P. administration.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID