Tamoxifen (ICI 46474) Citrate

For research use only.

Catalog No.S1972

10 publications

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.

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Selleck's Tamoxifen (ICI 46474) Citrate has been cited by 10 publications

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  • Effects of DPN, LY500307, Raloxifene and Tamoxifen on cell viability in BSO-treated FRDA fibroblasts. BSO concentration was 1 mM and all steroid concentrations were 100 nM. Depicted are mean ± SD for n= 8 per group. * indicated p<0.05 versus BSO alone-treated cells.

    University of North Texas Health Science Center, 2014.. Tamoxifen (ICI 46474) Citrate purchased from Selleck.

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Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.
Targets
ER [1]
(Cell-free assay)
In vitro

Tamoxifen displays antitumor effect due to its antiestrogenic activity (ER). Values for the apparent affinity of Tamoxifen for the ER range between 30 and 0.01% of that obtained for estradiol, dependent on different ER source (species), protein concentration and condition used for assay. Binding of Tamoxifen to ER further leads to inhibition expression of estrogen-regulated genes, including growth factors and angiogenic factors secreted by the tumor that may stimulate growth by autocrine or paracrine mechanisms. Tamoxifen also directly induces programmed cell death. [1] Tamoxifen produces an inhibitory effect on MCF-7 cell [3H]thymidine incorporation and DNA polymerase activity as well as causing a reduction in DNA content of cultures and cell numbers. This inhibitory effect of Tamoxifen on MCF-7 cell growth can be readily reversed by addition of estradiol to the culture medium. 2 and 6 μM Tamoxifen reduces the proportion of cells in S phase and increases the number of cells in G1. At 10 μM, Tamoxifen causes cell death within 48 hr. [2] Tamoxifen inhibits MCF-7 growth with IC50 of ~10 nM after 10 days treatment. Tamoxifen inhibits plasminogen activator activity of MCF-7, and suppresses estradiol-stimulation of plasminogen activator activity. Tamoxifen also evokes minimal increases in cellular progesterone receptor levels. [3] Tamoxifen is able to inhibit the growth of prostate cancer cell PC3, PC3-M, and DU145 with IC50 ranged from 5.5-10 μM, which is related to its inhibition of protein kinase C and induction of p21(waf1/cip1). [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells MnXGR5l1d3SxeHnjxsBie3OjeR?= NF:wU2gyQCCq Mli1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjCxPEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGwMlIh|ryP MnXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7M{KwNlQoRjF7OUOyNFI1RC:jPh?=
human MDA-MB-231 cells MUjDfZRwfG:6aXRCpIF{e2G7 MWOxPEBp NGLnTo1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUKuN{DPxE1? Mk\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7M{KwNlQoRjF7OUOyNFI1RC:jPh?=
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human DU145 cells NVfrOWpNS3m2b4TvfIlkyqCjc4PhfS=> NIrUOnIyQCCq MnvFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSHUyPDViY3XscJMh[W[2ZYKgNVghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xOU4zPSEQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
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MCF-7 MnXSSpVv[3Srb36gZZN{[Xl? Mle5NUB2VQ>? M4Lw[WlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBxem:uaX\ldoF1cW:wIH;mJG1ETi15IHPlcIx{KGmwIIDy[ZNmdmOnIH;mJFEx\S14IF2gZ49v[2WwdILheIlwdiCxZjDbN2heNSCnc4TyZYRqd2xiKFWyLUwhUUN|ME22MlXPxE1? NV2yW4N7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PVY5OSd-Mke2PVY5OTxxYU6=
OHS-50 MnnBdWhVWyCjc4PhfS=> NH3SNIVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz M{HuWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzh{MEG1PFcoRjh{MEG1PFc9N2F-
MCF-7 Ml3nR5l1d3O2YYTpZ4l1gSCjc4PhfS=> M{XMdFEhfU1? NYrNeoJLS3m2b4P0ZZRq[2m2eTDh[4FqdnO2IF3DSk04KGOnbHzzJIlvKHSqZTDwdoV{\W6lZTDv[kAyKHWPb3zhdkBGOiBqZYP0doFlcW:uKTygTWM2OD16zszN NYD2PVJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUC4PVM{Oid-OUC4PVM{OjxxYU6=
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MCF-7 M2rSWGN6fG:2b4jpZ4l1gSCjc4PhfS=> NU\aRYpDOSC3TR?= MkXFR5l1d3SxeHnjbZR6KGGpYXnud5QhVUOILUegZ4VtdHNiaX6gdJJme2WwY3Wgc4YhOSC3TTDFNkAp\XO2cnHkbY9tMSxiQ4n0c5RwgGmlaYT5QVE{|ryP Mk\GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTB6OUOzNkc,QTB6OUOzNlww[T5?
MCF-7 MYrGeY5kfGmxbjDhd5NigQ>? Mm\xTY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2rbnT1Z4VlKHC{b3zp[oVz[XSrb36gbY4hcHWvYX6gUWNHNTdiYoLlZZN1KGOjbnPldkBk\WyuczygTWM2OD1yLkS4Ne69VQ>? MlLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTF3NEm2N{c,QTF3NEm2N|ww[T5?
Ishikawa cells MYjBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> MnzKOEBl[Xm| MUjBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIV{fHKxZ3XuMYlv\HWlZXSgZYxs[WyrbnWgdIhwe3CqYYThd4Uh[WO2aY\peJkh[W[2ZYKgOEBl[Xm|IHL5JGVNUVODLDDJR|UxRTBwNENOwG0> NYHkU5hzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUe4OFE3Oyd-OUe4OFE3OzxxYU6=
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MCF-7-2a M1m1fWFvfGmnc4Tyc4dmdmmlIHHzd4F6 MV;BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDNR2YuPy1{YTDj[YxteyCjczDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIILl[JVk\SCnc4TyZYRqd2xiZX\m[YN1KGK7IEWwKUwhUUN3ME2wMlXPxE1? NWHKUFZwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0N|ExPjNpPkGyOFMyODZ|PD;hQi=>
MCF-7-2a MWTGeY5kfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGW|dILh[IlwdCCrbnT1Z4VlKGW|dILv[4VvKHKnY3XweI9zKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGmwIF3DSk04NTKjIHPlcIx{NCCLQ{WwQVAvPc7:TR?= NY\GXGJvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2O|IzPDlpPkGyOlczOjR7PD;hQi=>
SK-E2 NGDDNmRHfW6ldHnvckBie3OjeR?= MUDUVH9VWkGQU2DPVnRGWjpiaX7jdoVie2ViaX6gZo9lcXC7IHnueJJi[2WubIXsZZIh[WOldX31cIF1cW:wIDjCc4RqeHl8IECuNkB2VSliaX6gV2suTTJiY3XscJMhMGW6cILld5NqdmdiQmPFVEktKEmFNUC9NVUvPM7:TR?= M1;VVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O|U6Lz5zMkezPVc2QTxxYU6=
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DAOY NUXJcoNUeUiWUzDhd5NigQ>? NVLFb3VLeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= MmfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6MkW5N|UoRjF{OEK1PVM2RC:jPh?=
MCF-7 NX3hZ|R2TnWwY4Tpc44h[XO|YYm= NWLBZoVDUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgUWNHNTdiYoLlZZN1KHS3bX;yJINmdGy|IIXzbY5oKE2FRj23JIF{e2G7LDDJR|UxRTBwNUlOwG0> M3;QdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEK1PVM2Lz5zMkiyOVk{PTxxYU6=
Caco-2 M1KwTWZ2dmO2aX;uJIF{e2G7 NVnJZZlNXFChVGLBUnNRV1KWRWK6JJRz[W6|ZYDpeIhmdGmjbDD0doFve3CxcoSgc4YhWmixZHHtbY5mKDF{MzCoZoF{[WxidH:gZZBq[2GuKTDpckBE[WOxLUKgZ4VtdHNuIFnDOVA:OjoQvF2= MoTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV|OE[0PFIoRjF3M{i2OFgzRC:jPh?=
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MDA-MB 231 MmjORY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlPWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbY4hVUSDLV3CJFI{OSCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIx{NCCLQ{WwQVExNjcQvF2= M3qxUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFc2Lz5zNU[1PFg4PTxxYU6=
MCF7 MnfwSpVv[3Srb36gZZN{[Xl? NIPJbFA,OTBidV2= M2S4ZWFod26rc4SgZYN1cX[rdImgZZQhTVKjbIDoZUBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGUzNWmwZIXj[YQhdHWlaX\ldoF{\SCpZX7lJJRz[W6|YXP0bZZifGmxbjDpckBOS0Z5IHPlcIx{KGG2IE6xNEB2VQ>? M{S0dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{O1NVIxLz5zNkezOVEzODxxYU6=
MCF7 Mn2wRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2j1Z|MhdWdxa3e= M1;0VVQ5KGi{cx?= Mo\RRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNR2Y4KGOnbHzzJIF1KDNibXevb4cw\GG7IHHmeIVzKDR6IHjyd{whUUN3ME21MlA6|ryP MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl5OUOzO{c,OTZ7N{mzN|c9N2F-
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ER-positive MCF7 Mn7NRY51cWW|dILv[4VvcWNiYYPzZZk> MkDRNlAhdk1? M2TZSlQ5KGi{cx?= NV:2RWNtSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gSXIueG:|aYTpeoUhVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKDF5YnX0ZUBme3S{YXTpc4wucW6mdXPl[EB{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCjY4Tpeol1gSCjdDCyNEBvVSCjZoTldkA1QCCqcoOgZpkheGixc4DoZU1tcWeqdDDy[ZBwenSncjDjbIVucWy3bXnu[ZNk\W6lZTDhd5NigQ>? M1n3XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOECwPFU6Lz5{MUiwNFg2QTxxYU6=
HEK293 NUHjTHUxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYq0PEBpenN? NH:3NpNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjFT|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGw{txO MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5MUixNkc,OjF6N{G4NVI9N2F-
MCF7 MYjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MXK0PEBpenN? MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{LkS4{txO MnfGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MDA-MB-231 MUjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M1GyVlQ5KGi{cx?= NXrvPW1sSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDlfJBz\XO|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUiuPe69VQ>? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5MUixNkc,OjF6N{G4NVI9N2F-
Ishikawa Ml;mRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NHW4SYM1QCCqcoO= Ml36RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BmgHC{ZYPzbY5oKGW|dILv[4VvKHKnY3XweI9zKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlIvPc7:TR?= MlzqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
A673 NH7ycmNyUFSVIHHzd4F6 MVTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MkC0OUc,OjJ2N{KwOFU9N2F-
HEK293 MUfGeY5kfGmxbjDhd5NigQ>? M1LHflEvPSCqcoO= NVThSZI3TGm|cHzhZ4Vu\W62IH;mJHs{UF2OU1Sg[pJwdSCqdX3hckA2UFR4IILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOS53IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4k:OS5yNEJOwG0> NVfmWZZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1N|cyPTNpPkKyOVM4OTV|PD;hQi=>
AH109 NEDodGNHfW6ldHnvckBie3OjeR?= NWK4cYh6OjRiaILz MXnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KEWUYnX0ZUBz\WOncITvdkBNSkRiZYjwdoV{e2WmIHnuJJlm[XO2IFHINVA6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gbY51\XKjY4Tpc44hf2m2aDDTVmMyKGGodHXyJFI1KGi{czDifUBidHCqYT3nZYxi[3Sxc3nkZZNmKGG|c3H5MEBKSzVyPUGuOlbPxE1? MmP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
AH109 MVrGeY5kfGmxbjDhd5NigQ>? M2fPdFI1KGi{cx?= NVHKXWR3SW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIILlZ4VxfG:{IFzCSEBmgHC{ZYPz[YQhcW5ieXXhd5QhSUhzMEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTldoFkfGmxbjD3bZRpKFOUQ{GgZYZ1\XJiMkSgbJJ{KGK7IHHsdIhiNWejbHHjeI9{cWSjc3WgZZN{[XluIFnDOVA:Oi53NN88US=> NWG4WodnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
MCF7 MlPhRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M{LjflI1KGi{cx?= NWXFTG5uSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQS53NN88US=> NGXNcZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O|IyPyd-MkK2OFczOTd:L3G+
DLD1 MkXPR5l1d3SxeHnjbZR6KGG|c3H5 MVK5OkBpenN? NYLRNY9GS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTEyGMTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVQvPzkQvF2= NFLWRmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
MCF7 M1ezV2N6fG:2b4jpZ4l1gSCjc4PhfS=> NX72RYdKQTZiaILz NUDlPZJuS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOc7:TR?= NX;wTJR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
NIH/3T3 Mof1R5l1d3SxeHnjbZR6KGG|c3H5 MkP4PVYhcHK| M4TvSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9|VEOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjJ4zszN MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
518A2 NWPB[XdrS3m2b4TvfIlkcXS7IHHzd4F6 NInvfZc6PiCqcoO= NEHyc2hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA2OTiDMjDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvPjMQvF2= NGDpRYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
A2780 M{TYdGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVjHN3duQTZiaILz MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUeuO|fPxE1? Mn;mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
Lipo MknPR5l1d3SxeHnjbZR6KGG|c3H5 MXi5OkBpenN? MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD62OO69VQ>? NXz6fmF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
A253 NFGw[5ZEgXSxdH;4bYNqfHliYYPzZZk> MljkPVYhcHK| MkSyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI2OyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUiuPVLPxE1? NWnkfYFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
A549 NVznZ4hPS3m2b4TvfIlkcXS7IHHzd4F6 NVHXTZFrQTZiaILz NUfkNIY1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVkvPjcQvF2= Mn7qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
850C MU\DfZRwfG:6aXPpeJkh[XO|YYm= M2T5XFk3KGi{cx?= M4\RZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFg2OENiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2xNU4xQc7:TR?= M2\lUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
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MCF7 Mm\4R5l1d3SxeHnjbZR6KGG|c3H5 MlXqO|IhcHK| M{n6fWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHdUXDFiYYPzZZkhcW5icILld4Vv[2Vib3[gNlAmKE[EUzygTWM2OD16LkdOwG0> M2rhUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUCxPFk2Lz5{MkmwNVg6PTxxYU6=
HL60 NE\uU|FEgXSxdH;4bYNqfHliYYPzZZk> MUS3NkBpenN? MnrFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVOSCjc4PhfUBqdiCycnXz[Y5k\SCxZjCyNEUhTkKVLDDJR|UxRTF2LkROwG0> NVH6flZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5NFE5QTVpPkKyPVAyQDl3PD;hQi=>
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C41 DE3 NHnwd4FHfW6ldHnvckBie3OjeR?= M{DQPVYxKHWP M4r6Rmlv[WO2aY\heIlwdiCxZjDDXXAzSjZiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDDOFEhTEV|IHPlcIx{KGG|c3Xzd4VlKGG|IH3v[IlncWWmIHHwc{1RPDVyIELCOkBi\GS3Y4Sg[o9zdWG2aX;uJIF1KDZyIIXNJIJ6KEWVST3MR{1OWyCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBPSUSSSDDhcoQhT1OKRVW= NUO4bJJ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OFI{OTdpPkKyPVQzOzF5PD;hQi=>
C41 DE3 NEfYZ2VHfW6ldHnvckBie3OjeR?= MXixNFAhfU1? MVuzNEBucW6| MnXaTY5i[3SrdnH0bY9vKG:oIFPZVFJDPiBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFO0NUBFTTNiY3XscJMh[XO|ZYPz[YQh[XNidIL5dJRq[yCyZYD0bYRmKDF6OF\IXXFFWUVzOUSge4l1cCC|aYTlJI9nKGGmZIXjeEBnd3KvYYTpc44hdG:lYXzpfoVlKHSxIFfsclE6OyCjdDCxNFAhfU1iYX\0[ZIhOzBibXnud{BjgSCOQz3NV{9OWyCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBPSUSSSB?= NVz3XHRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OFI{OTdpPkKyPVQzOzF5PD;hQi=>
MCF7 NXfNe5BHS3m2b4TvfIlkcXS7IHHzd4F6 NEmwPVI1QCCqcoO= MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUGuOFTPxE1? NX\QPHV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlIzQDFpPkKzNFIzOjhzPD;hQi=>
MDA-MB-231 Mn;tR5l1d3SxeHnjbZR6KGG|c3H5 MY[0PEBpenN? NGe3O25EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUKuOFHPxE1? M{[0UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEKyNlgyLz5{M{CyNlI5OTxxYU6=
MCF7 M4LLS2N6fG:2b4jpZ4l1gSCjc4PhfS=> NHK1Row1QCCqcoO= M1jidWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFnDOVA:OS56Mt88US=> MnjEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzMkOwNVcoRjJ|MUKzNFE4RC:jPh?=
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HeLa Ml:5SpVv[3Srb36gZZN{[Xl? MmDaNlQhcHK| NFq4eVlCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IH\1cIwhdGWwZ4ToJGVT[WyyaHGgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDFVmUu\HKrdnXuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjN2Md88US=> NGLTPY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEi4NlUxOid-Mki4PFI2ODJ:L3G+
HeLa NEnWbXdHfW6ldHnvckBie3OjeR?= MV[yOEBpenN? MYnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KG[3bHygcIVv\3SqIFXSZoV1[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCKZVzhJINmdGy|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEWURT3kdol3\W5ibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUGuOVPPxE1? M2C4VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEiyOVAzLz5{OEi4NlUxOjxxYU6=
MCF7 NFT3RlVEgXSxdH;4bYNqfHliYYPzZZk> NWfKbGgyPzJiaILz M1LTWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JJJme2G8dYLpckBj[XOnZDDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTB;Nz65OO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{e5OUc,Ojh7Mke3PVU9N2F-
insect cells MlTUSpVv[3Srb36gZZN{[Xl? NFHWS5EzKGi{cx?= NXe3[Zl1TGm|cHzhZ4Vu\W62IH;mJIZtfW:{ZYPj[Y51KGW|dILv[4VvKGyrZ3Hu[EBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyCrbnP1ZoF1\WRiZn;yJFIhcHK|IHL5JJBwdGG{aYrheIlwdixiSVO1NF0zNjZ5zszN MlLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEKxNVMoRjJ6OUSyNVE{RC:jPh?=
MCF7 NXPD[Xh4S3m2b4TvfIlkcXS7IHHzd4F6 MXG0PEBpenN? Mlj1R5l1d3SxeHnjbZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJIdzd3e2aDDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTRwM{ZOwG0> M1\OflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUSyNVE{Lz5{OEm0NlEyOzxxYU6=
Ishikawa cells NX3BNItMS3m2b4TvfIlkcXS7IHHzd4F6 NHTCfGE1QCCqcoO= NHzxW3dEgXSxdH;4bYNqfHliaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlAvPjYQvF2= NXftcG1nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
MCF7 M3\Q[GZ2dmO2aX;uJIF{e2G7 NYTBTIRLOTBibl2= MlvkNlQhcHK| NInYR|lKdmirYnn0bY9vKG:oIFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iMUCgcm0h\XO2cn;n[Y4ucW6mdXPl[EBxem:pZYP0[ZJwdmVicnXj[ZB1d3JiZYjwdoV{e2mxbjDheEAyKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDt[ZRpd2R? NHnUUYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
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U2O2 Mk\ISpVv[3Srb36gZZN{[Xl? NGTtN2gyKHWP NF33eo41KGi{cx?= NHHuW|ZKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFfGVE1nfXOnZDDFVoFteGijIFzCSEApOzBzIITvJFU2OyC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJIh2dWGwIGWyU|Ih[2WubIOgZZN{\XO|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhOTekZYThMYV{fHKjZHnvcE1qdmS3Y3XkJJRz[W6|Y4LpdJRqd25iYYSgNUB2VSCycnXpcoN2[mG2ZXSg[o9zKDRiaILzJIZwdGyxd3XkJIJ6KEV{IHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgQ>? MkTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyNEWxN|UoRjJ7MES1NVM2RC:jPh?=
MCF7 M3n2UmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGTNZ4M1QCCqcoO= MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF05NjF{zszN NEDuRWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
MDA-MB-231 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnvpOFghcHK| MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME25Mlk3|ryP NH[4XI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
HEK293 MU\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYHvNZhRPDhiaILz NGXWNnNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGhGUzJ7MzDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVIxNjl|zszN NHTMWWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
MCF7 NG\kVndE\WyuIHP5Z4xmKGG|c3H5 M3XnZVExKHWP MUWyOEB1dyB2ODDodpM> MWLD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOC:JMTDwbIF{\SCjdDCxNEB2VSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=> M3;pTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEW0N|U6Lz5{OUC1OFM2QTxxYU6=
MCF7 NWHac3dPS3m2b4P0ZZRq[yCjY4Tpeol1gSCjc4PhfS=> NVXkSIVpS3m2b4P0ZZRq[yCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMtKEmFNUC9Nk44|ryP Mk\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MEezN|UoRjJ7MkC3N|M2RC:jPh?=
MCF7 MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVq3NkBpenN? M3HBZWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21MlPPxE1? MkDkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MkC3PFkoRjJ7MkKwO|g6RC:jPh?=
MCF7 MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NV3kbYZWPDhiaILz NV\0d4ppSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUmuOe69VQ>? NX;tT2VnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PFI6PDRpPkK5OFgzQTR2PD;hQi=>
Ishikawa cells NH;G[3VCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGPNNZE,PDBidV2= NX\J[YVGPDhiaILz MmrORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BifCB-NECgeW0h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfS=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0OEc,Ojl2OEK5OFQ9N2F-
MCF7 MVzGeY5kfGmxbjDhd5NigQ>? NEPMZowyKHWP NF7RXlRCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iZYP0doFlcW:uLXnu[JVk\WRiUHfSJI1TVkFiZYjwdoV{e2mxbjDheEAyKHWPIHL5JHN[SlJiR4Ll[Y4h\HmnIHLhd4VlKFKWLWDDVkBidmGueYPpdy=> NIrafWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS4Nlk1PCd-Mkm0PFI6PDR:L3G+
MCF7 MlrVSpVv[3Srb36gZZN{[Xl? MVSyOEBpenN? MmPSTY5lfWO2aX;uJI9nKHOnbHXjeIl3\SCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZUBl\We{YXTheIlwdiCrbjDoeY1idiCPQ1[3JINmdGy|IHjhdoJwemmwZzDUT{1GWkVvTIXjJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDld5Rz[WSrb3ytbY5lfWOnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1zLk[wOe69VQ>? NEjpN4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2Nlc{Pyd-Mkm1OlI4Ozd:L3G+
MCF7 MlLVSpVv[3Srb36gZZN{[Xl? M1Xie|YxKG2pL3vn MYm0PEBl[Xm| NGjl[WlFd3ewcnXneYxifGmxbjDv[kBxem:pZYP0[ZJwdmVicnXj[ZB1d3JibWLORUBt\X[nbDDpckBPV0RiU1PJSEBo[W2vYTDtc5V{\SC6ZX7v[5Ji\nSnZDD3bZRpKGi3bXHuJG1ETjdiY3XscJMh[XRiNkCgcYcwc2duIIDvJFUh\GG7czDw[ZIhf2WnazDmc5IhPDhiZHH5d{Bxd3O2IIT1cY9zKGmvcHzhcpRifGmxbjDt[YF{fXKnZDC3JIhzeyCyb4P0JIxie3RiZH;z[UBjgSCUVD3QR3IhdWW2aH;k M2TzZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NU[yO|M4Lz5{OUW2Nlc{PzxxYU6=
insect cells MmrXSpVv[3Srb36gZZN{[Xl? NUTHT4pXOiCqcoO= NHXuO21FcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIH\1cIwhdGWwZ4ToJIh2dWGwIFXSMYFteGijIHX4dJJme3OnZDDpckBqdnOnY4SgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OD1zLkSy{txO MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6N{KyNUc,Ojl3OEeyNlE9N2F-
MCF7 Ml;wRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlixOFghcHK| NX\McWRQSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUWuOFbPxE1? NU\EbnppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFczOjFpPkK5OVg4OjJzPD;hQi=>
Ishikawa cells MlXqRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1zLTlQ5KGi{cx?= M3K0UmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSYPobYtif2FiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yOk42Os7:TR?= NGj4VJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4O|IzOSd-Mkm1PFczOjF:L3G+
MRC5 SV2 M1G5eGN6fG:2b4jpZ4l1gSCjc4PhfS=> MnnmO|IhcHK| M1\sUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViU2[yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSC{ZYPhfpVzcW5iZInlMYJie2WmIH\seY9zcW2ndILpZ{BidmGueYPpd{whUUN3ME2xNE4z|ryP MknLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4MES1OFEoRjJ7NkC0OVQyRC:jPh?=
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insect NV;Ye|NsTnWwY4Tpc44h[XO|YYm= NIXVcGlDcW6maX7nJIFn\mmwaYT5JJRwKHWwdHHn[4VlKGi3bXHuJJJm[2:vYnnuZY51KG[3bHygcIVv\3SqIFXSZYxxcGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyByLkGgeI8hOTByMECgeW0h[nliRnz1c5Jud26nIFXTNkBIemWnbjDkbZNxdGGlZX3lcpQh[mG|ZXSgZZN{[Xl? NWTEVmdORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwPVE6OjBpPkOwNFkyQTJyPD;hQi=>
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MDA-MB-231 MoH3R5l1d3SxeHnjbZR6KGG|c3H5 MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|LDDHTVUxRTBwMkVOwG0> MofvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{MECyN|YoRjNzMkCwNlM3RC:jPh?=
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MCF7 MXfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MonVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE45P87:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV2NkG5O{c,OzF3NE[xPVc9N2F-
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HeLa NVLoSGNsSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NH23blRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVExNjh5zszN MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV2NkG5O{c,OzF3NE[xPVc9N2F-
DU145 MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MmLrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2= MnTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3NE[xPVcoRjNzNUS2NVk4RC:jPh?=
MDA-MB-231 M4PhZmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTFzLkNOwG0> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV2NkG5O{c,OzF3NE[xPVc9N2F-
SiHa NWP6VXFzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljsS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gV4lJ[SClZXzsd{whT0l3ME2wMlEz|ryP Mmj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MEW4OlUoRjNzNkC1PFY2RC:jPh?=
MDA-MB-231 NFS1ZZdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFO0TYVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOTEFvTVKtNlMyKGOnbHzzMEBIUTVyPUCuNVLPxE1? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZyNUi2OUc,OzF4MEW4OlU9N2F-
PANC1 NWTKfGJVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml3rS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yOs7:TR?= M2jCR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkC1PFY2Lz5|MU[wOVg3PTxxYU6=
MCF7 NXHrbnMxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MmLtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHL5JIZtfW:{ZYPj[Y5k\SCkYYPl[EB3cWGkbHWgZ4VtdCClb4XueIlv\yCvZYToc4QtKEmFNUC9NlXPxE1? M17ye|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkGwN|c5Lz5|MU[xNFM4QDxxYU6=
M-HeLa NF[5WoRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE1vSHXMZUBk\WyuczDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVI5|ryP M2DwdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkGwN|c5Lz5|MU[xNFM4QDxxYU6=
Chang NVPqUnp2S3m2b4TvfIlkcXS7IHHzd4F6 NYfESlNjS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS2ijbnegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIJ6KG[udX;y[ZNk\W6lZTDiZZNm\CC4aXHicIUh[2WubDDjc5VvfGmwZzDt[ZRpd2RuIFnDOVA:PDJwMd88US=> MkXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MUCzO|goRjNzNkGwN|c5RC:jPh?=
MCF7 M3zCZWN6fG:2b4jpZ4l1gSCjc4PhfS=> MYe0PEBpenN? NUj4fIxnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTJ{LkdOwG0> NWTLcYlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G2NlAzOjVpPkOxOlIxOjJ3PD;hQi=>
RAW264.7 NGP2OnNEgXSxdH;4bYNqfHliYYPzZZk> MXKyOEBpenN? M{ThXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjCyOEBpenNiYomgcoV2fHKjbDDy[YQh\HmnIHLhd4VlKGG|c3H5MEBESzVyPUGxMlHPxE1? NWPFdJp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3NFM5OThpPkOxO|A{QDF6PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT ; 

PubMed: 30759864     


MCF-7 cells were treated with 2 μM tamoxifen and harvested at the indicated time point to analyze the expressions of p-AKT and AKT by western blot.

ER-α66 / ER-α36 / HER2 / EGFR ; 

PubMed: 25203051     


Western blot analysis of the expression levels of ER-α66, ER-α36, HER2 and EGFR in ER-positive breast cancer MCF7 (A), T47D (B) and H3396 (C) cells treated with 1 µM of tamoxifen (TAM) for indicated time period.

p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; 

PubMed: 27020861     


B. Immunoblot of AMPK pathway activation following treatment with 30μM tamoxifen in MCF7 and C. in MDA-MB-231 cells. 

CD36 / p-ERK / ERK ; 

PubMed: 30573731     


MCF-7 cells MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h, or with 5 μM Tam for the indicated times. Expression of CD36, p-ERK1/2 and ERK1/2 was determined by western blot.

30759864 25203051 27020861 30573731
Immunofluorescence
CD36; 

PubMed: 30573731     


MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h. Expression of CD36 was determined by immunofluorescent staining . 

30573731
In vivo

Tamoxifen administration to rapidly growing, estradiol-stimulated MCF-7 xenografts results in a dose-dependent retardation or cessation of tumor growth by significantly decreasing tumor cell proliferation in tumor. Tamoxifen treatment results in a slowing of tumor growth (tumor doubling time, 12 days), a significant increase in tumor potential doubling time (Tpot) (6.6 days), and a decrease in labeling index (%LI) (to 8%) by 23 days posttreatment, compared with untreated mice which shows a volume doubling time of 5 days, a Tpot of 2.3 days, and a %LI of 23%. [5] Tamoxifen has not only antiestrogenic but also estrogenic properties depending on the species, tissue, and gene. Tamoxifen displays favorable effects on bone and serum lipid concentrations and stimulation endometrium. [1]

Protocol

Kinase Assay:

[3]

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Competitive binding assays:

Cells are harvested from 150-sq cm T-flasks, and cytosol is prepared at a protein concentration of approximately 2 mg/mL in phosphate buffer. Aliquots of this 180,000 ×g supernatant are then incubated with various concentrations of Tamoxifen and 2.5 nM [3H]estradiol for 16 hr at 0-4 ℃. The free steroids are absorbed by dextran-charcoal [l0 μL of 0.5% Dextran C-5% Norite A in TE buffer] for 1 hr at 0 ℃, and aliquots are counted after centrifugation at 800 ×g, 30 min. The relative binding ability of each competitor is taken as the ratio of the concentration of radioinert estradiol/competitor required to inhibit one-half of the specific [3H]estradiol binding, with the affinity of estradiol set at 100%.
Cell Research:

[3]

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  • Cell lines: Human breast cancer cells MCF-7
  • Concentrations: ~1 μM
  • Incubation Time: 10 days
  • Method:

    MCF-7 cells are seeded into T-25 flasks (1.5×105 cells/flask) and grown for 2 days in the MEM supplemented with 10 mM HEPES buffer, gentamicin (50 μg/mL), penicillin (100 units/mL), streptomycin (0.1 mg/mL), bovine insulin (6 ng/mL), hydrocortisone (3.75 ng/mL), and 5% calf serum that has been treated with dextran-coated charcoal for 45 min at 55 ℃ to remove endogenous hormones. The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen. At the end of incubation, cell numbers are counted.


    (Only for Reference)
Animal Research:

[5]

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  • Animal Models: Human breast carcinoma xenografts MCF-7
  • Dosages: 2 cm Tamoxifen capsules
  • Administration: Subcutaneous implantation
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (177.41 mM)
Water Insoluble
Ethanol ''''100 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.64
Formula

C26H29NO.C6H8O7

CAS No. 54965-24-1
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3.C(C(=O)O)C(CC(=O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester May 1 2021 Phase 1
NCT04433494 Not yet recruiting Drug: TY-302: capsule 25mg/50mg ; Tamoxifen: tablet10mg Breast Cancer|Solid Tumor TYK Medicines Inc October 2020 Phase 1
NCT04174352 Recruiting Drug: Tamoxifen|Diagnostic Test: FES PET/CT ERα+ Breast Cancer|ESR1 Gene Mutation University of Wisconsin Madison October 20 2020 Early Phase 1
NCT04312347 Recruiting Drug: Tamoxifen dose adjustment ER+ Breast Cancer Nalagenetics Pte Ltd September 6 2019 Not Applicable

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?

  • Answer:

    The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID