Tamoxifen (ICI 46474) Citrate

Catalog No.S1972

For research use only.

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Selleck's Tamoxifen (ICI 46474) Citrate has been cited by 14 publications

Purity & Quality Control

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Biological Activity

Description Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.
Targets
ER [1]
(Cell-free assay)
In vitro

Tamoxifen displays antitumor effect due to its antiestrogenic activity (ER). Values for the apparent affinity of Tamoxifen for the ER range between 30 and 0.01% of that obtained for estradiol, dependent on different ER source (species), protein concentration and condition used for assay. Binding of Tamoxifen to ER further leads to inhibition expression of estrogen-regulated genes, including growth factors and angiogenic factors secreted by the tumor that may stimulate growth by autocrine or paracrine mechanisms. Tamoxifen also directly induces programmed cell death. [1] Tamoxifen produces an inhibitory effect on MCF-7 cell [3H]thymidine incorporation and DNA polymerase activity as well as causing a reduction in DNA content of cultures and cell numbers. This inhibitory effect of Tamoxifen on MCF-7 cell growth can be readily reversed by addition of estradiol to the culture medium. 2 and 6 μM Tamoxifen reduces the proportion of cells in S phase and increases the number of cells in G1. At 10 μM, Tamoxifen causes cell death within 48 hr. [2] Tamoxifen inhibits MCF-7 growth with IC50 of ~10 nM after 10 days treatment. Tamoxifen inhibits plasminogen activator activity of MCF-7, and suppresses estradiol-stimulation of plasminogen activator activity. Tamoxifen also evokes minimal increases in cellular progesterone receptor levels. [3] Tamoxifen is able to inhibit the growth of prostate cancer cell PC3, PC3-M, and DU145 with IC50 ranged from 5.5-10 μM, which is related to its inhibition of protein kinase C and induction of p21(waf1/cip1). [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells MUHDfZRwfG:6aXRCpIF{e2G7 MUmxPEBp NWS0R2JFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkAyQCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVExNjJizszN M2HHR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOyNFI1Lz5zOUmzNlAzPDxxYU6=
human MDA-MB-231 cells NWjZRVNoS3m2b4TvfIlkyqCjc4PhfS=> M{DyOFE5KGh? NEPtcXlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUKuN{DPxE1? M3XjTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOyNFI1Lz5zOUmzNlAzPDxxYU6=
human Ishikawa cells M2HRb2N6fG:2b4jpZ:Kh[XO|YYm= MlvSNVghcA>? NFqzZW5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBKe2ira3H3ZUBk\WyuczDh[pRmeiBzODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{LkWg{txO MonyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7M{KwNlQoRjF7OUOyNFI1RC:jPh?=
human DU145 cells MkLtR5l1d3SxeHnjxsBie3OjeR?= NV3Tc2Z4OThiaB?= NWjTT2M1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPS5{NTFOwG0> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
MCF-7 NF7wTpdHfW6ldHnvckBie3OjeR?= M1;WV2lvcGmkaYTpc44hd2ZiW{PIYZRpgW2rZHnu[UBqdmOxcoDvdoF1cW:wIHnueI8hVUOILUegZ4VtdHNuIFnDOVA:Py5{zszN M{jZXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{O2N|AoRjF3N{O2N|A9N2F-
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Ishikawa cells MVfBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> M1jVXlQh\GG7cx?= NWf5PVRtSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gTZNpcWujd3GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2rbnT1Z4VlKGGua3HsbY5mKHCqb4PwbIF1[XOnIHHjeIl3cXS7IHHmeIVzKDRiZHH5d{BjgSCHTFnTRUwhUUN3ME2wMlQz|ryP NIDlOGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97N{i0NVY{Lz57N{i0NVY{RC:jPh?=
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MCF-7 NEDI[HVHfW6ldHnvckBie3OjeR?= MkXtTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiTVPGMVch[nKnYYP0JJR2dW:{IHPlcIx{KHW|aX7nJG1ETi15IHHzd4F6NCCLQ{WwQVAvPTkQvF2= MnjVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6MkW5N|UoRjF{OEK1PVM2RC:jPh?=
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MDA-MB 231 NED0ZmtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDpckBOTEFvTVKgNlMyKGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscJMtKEmFNUC9NVAvPs7:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi3OUc,OTV4NUi4O|U9N2F-
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MCF7 NYnlW2lCS3m2b4TvfIlkcXS7IHHzd4F6 NIXaSIY3KHWP MlrSPVYhcHK| MmLGR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIF1KDZidV2gZYZ1\XJiOU[gbJJ{KGK7IF3UWEBie3OjeR?= NUHtdpd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NlE1QTJpPkKwOlIyPDl{PD;hQi=>
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MCF7 NVvRXpliSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MoTQOFghcHK| NXzQcWNXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE4xODNibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlAyKG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW|ZX7j[UBw\iB3JTDm[ZRidCCkb4\pcoUhe2W{dX2sJGdKPTB;Mj6yPVA5P87:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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MCF7 M4H6[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MoLZOFghcHK| NUGzcmhySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNVAxKG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4Yh\mW2YXygZo93cW6nIIPldpVuKGGwZDDOeXNmenWvLDDHTVUxRTVwNkKzOFHPxE1? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
NCI-ADR-RES NHfU[olCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MmXaOFghcHK| MnjyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC5yMEOgcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjBzIH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjC1KUBn\XSjbDDic5ZqdmVic3XyeY0tKEeLNUC9OU44PTR2zszN MnPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
MCF12A M3ryXGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MkjjOFghcHK| MlnVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIECuNFA{KG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wNUBvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4YhPSViZnX0ZYwh[m:4aX7lJJNmenWvLDDHTVUxRTVwN{W0OO69VQ>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
MCF12A NFHQZpdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFzkfIo1QCCqcoO= MkSyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTVPGNVJCKGOnbHzzJIlv[3WkYYTl[EB4cXSqIEGwNEBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF02Njd3NEVOwG0> NWnrdodDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
MCF12A NIPDSWNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MoLwOFghcHK| M2qyVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF3DSlEzSSClZXzsd{BqdmO3YnH0[YQhf2m2aDCwMlA3KG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4Yh\mW2YXygZo93cW6nIIPldpVuKGGwZDDOeXNmenWvLDDHTVUxRTZwOUG4N|HPxE1? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
NCI-ADR-RES NYPmU5VDSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M{CxOlQ5KGi{cx?= Mm\0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC5yNjDuUUBw\iCnc4TyZYRqd2xiYX7kJFAvODdibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIH\leIFtKGKxdnnu[UB{\XK3bTDhcoQhVnWVZYL1cUwhT0l3ME25MlU1QTl|zszN MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
LNCAP M33kemFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MXS0PEBpenN? MnnZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOTlPBVEBk\WyuczDlfJBz\XO|aX7nJIFv\HKxZ3XuJJJm[2WydH;yJIlv[3WkYYTl[EB4cXSqIECuOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwNzDuUUBw\iC2ZYP0c5N1\XKxbnWgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUC9NVDPxE1? NX\3XVk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
PC3 NFy2[YJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1LxUlQ5KGi{cx?= NEDMdHRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIFv\HKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDQR|Mh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD62JI5OKG:oIHXzeJJi\GmxbDDhcoQhOC55IH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3ME2xNE46PjR6zszN NVPNUYN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
NCI-ADR-RES NWrVWHdESW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYXZXodzPDhiaILz Mmi5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDld5Rzd2enbjDy[YNmeHSxcj3k[YZq[2mnboSgbJVu[W5iTlPJMWFFWi2URWOgZ4VtdHNiaX7jeYJifGWmIIfpeIghOTByIH7NJI9nKGW|dILh[IlwdCCjbnSgNE4xPyCwTTDv[kB1\XO2b4P0[ZJwdmViYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiZnX0ZYwh[m:4aX7lJJNmenWvIHHu[EBPfVOncoXtMEBIUTVyPUGxMlQ5OTYQvF2= MoHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO|UoRjJzNUGzNlc2RC:jPh?=
DU145 MnrNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUe0PEBpenN? MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHu[JJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCGVUG0OUBk\WyuczDpcoN2[mG2ZXSge4l1cCByLk[gcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjdibl2gc4YhfGW|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUGzMlgxOzkQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO|U9N2F-
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ER-positive MCF7 M1zPRWFvfGmnc4Tyc4dmdmmlIHHzd4F6 M4\QZ|IxKG6P MlXxOFghcHK| NUTQW292SW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gSXIueG:|aYTpeoUhVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKDF5YnX0ZUBme3S{YXTpc4wucW6mdXPl[EB{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCjY4Tpeol1gSCjdDCyNEBvVSCjZoTldkA1QCCqcoOgZpkheGixc4DoZU1tcWeqdDDy[ZBwenSncjDjbIVucWy3bXnu[ZNk\W6lZTDhd5NigQ>? NFvzc4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiwNFg2QSd-MkG4NFA5PTl:L3G+
HEK293 NYnQcIRlSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEfUcpg1QCCqcoO= M3THPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUFOwG0> NXLuOFQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O|E5OTJpPkKxPFcyQDF{PD;hQi=>
MCF7 M{PpTWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NULKSI83PDhiaILz Mmm4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj60PO69VQ>? NIrTZpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3NVgyOid-MkG4O|E5OTJ:L3G+
MDA-MB-231 MnS5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1vkcFQ5KGi{cx?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGW6cILld5NqdmdiZYP0do9o\W5icnXj[ZB1d3JiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQC57zszN M3\OdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEexPFEzLz5{MUi3NVgyOjxxYU6=
Ishikawa MojFRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NEnkPHA1QCCqcoO= NFzjc5JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGW6cILld5NqdmdiZYP0do9o\W5icnXj[ZB1d3JiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zOi53zszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh5MUixNkc,OjF6N{G4NVI9N2F-
A673 MWrxTHRUKGG|c3H5 MofndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MkC0OUc,OjJ2N{KwOFU9N2F-
HEK293 NFrXbGZHfW6ldHnvckBie3OjeR?= NUTPSJZtOS53IHjydy=> NXXFbVBjTGm|cHzhZ4Vu\W62IH;mJHs{UF2OU1Sg[pJwdSCqdX3hckA2UFR4IILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOS53IHjyd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4k:OS5yNEJOwG0> NFTlcpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWzO|E2Oyd-MkK1N|cyPTN:L3G+
AH109 NVXKXJFsTnWwY4Tpc44h[XO|YYm= NHnV[pozPCCqcoO= NFXrNFlCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZoV1[SC{ZXPldJRweiCOQlSg[ZhxemW|c3XkJIlvKHmnYYP0JGFJOTB7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCLQ{WwQVEvPjcQvF2= NXzKc4V6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
AH109 M1jP[WZ2dmO2aX;uJIF{e2G7 Ml2zNlQhcHK| Mo\mRY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKHKnY3XweI9zKEyERDDlfJBz\XO|ZXSgbY4hgWWjc4SgRWgyODliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBqdnSncnHjeIlwdiC5aYToJHNTSzFiYX\0[ZIhOjRiaILzJIJ6KGGucHjhMYdidGGldH;zbYRie2ViYYPzZZktKEmFNUC9Nk42PM7:TR?= NWWx[mVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
MCF7 NHfWNGVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M37ZZVI1KGi{cx?= MoS2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEB3cWGkaXzpeJkh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPU42PM7:TR?= MnrsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
DLD1 NVqye4dVS3m2b4TvfIlkcXS7IHHzd4F6 M4rUR|k3KGi{cx?= NHfoZVVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFVERzIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9OE44QM7:TR?= NFfuPWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
MCF7 NWewNnRkS3m2b4TvfIlkcXS7IHHzd4F6 NEPtdZQ6PiCqcoO= MU\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;Nz6x{txO MmTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
NIH/3T3 NYHkfW1ZS3m2b4TvfIlkcXS7IHHzd4F6 MmqwPVYhcHK| M2XZPWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9|VEOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjJ4zszN MnG3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
518A2 NXjL[INSS3m2b4TvfIlkcXS7IHHzd4F6 MVW5OkBpenN? MXXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUeuOlLPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
A2780 MVPDfZRwfG:6aXPpeJkh[XO|YYm= M1fYT|k3KGi{cx?= NEXaZWxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvPzgQvF2= NHmzSpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
Lipo M3Kwb2N6fG:2b4jpZ4l1gSCjc4PhfS=> MX[5OkBpenN? MVnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD62OO69VQ>? MnrDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
A253 NELwXlZEgXSxdH;4bYNqfHliYYPzZZk> NYXtcWp4QTZiaILz MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlU{KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD65Nu69VQ>? M2qyTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
A549 M2KyV2N6fG:2b4jpZ4l1gSCjc4PhfS=> MVe5OkBpenN? MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OT62Ou69VQ>? M4LiZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
850C M2r0dmN6fG:2b4jpZ4l1gSCjc4PhfS=> NWXX[mRKQTZiaILz NH\5PHNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA5PTCFIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NVEvODoQvF2= NFHoSm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
KIF5 NFS0Zm5Vd3irY3n0fUBie3OjeR?= M3i4fVI1KGi{cx?= MWDUc5hq[2m2eTDh[4FqdnO2IHj1cYFvKEuLRkWgZ4VtdHNiaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhS2WubGTpeIVzKEKudXWgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvPzYQvF2= M2HQRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{e1OFc1Lz5{Mke3OVQ4PDxxYU6=
MCF7 NX24[HlpS3m2b4TvfIlkcXS7IHHzd4F6 NHjqS2c4OiCqcoO= MnLhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVOSCjc4PhfUBqdiCycnXz[Y5k\SCxZjCyNEUhTkKVLDDJR|UxRThwNt88US=> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjlyMUi5OUc,OjJ7MEG4PVU9N2F-
HL60 MmTrR5l1d3SxeHnjbZR6KGG|c3H5 NG\ZWmE4OiCqcoO= NXixWFFlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhX1OWMTDhd5NigSCrbjDwdoV{\W6lZTDv[kAzOCViRlLTMEBKSzVyPUG0MlPPxE1? MkT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MEG4PVUoRjJ{OUCxPFk2RC:jPh?=
C41 DE3 MU\GeY5kfGmxbjDhd5NigQ>? MonNOlAhfU1? MXyxNEBucW6| NFLSNnhKdmGldHn2ZZRqd25ib3[gR3lROkJ4IDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgR|QyKESHMzDj[YxteyCjc4Pld5Nm\CCjczDtc4Rq\mmnZDDhdI8uWDR3MDCyRlYh[WSmdXP0JIZwem2jdHnvckBifCB4MDD1UUBi\nSncjCxNEBucW6|IHL5JGVUUS2OQz3NV{BidmGueYPpd{BqdiCycnXz[Y5k\SCxZjDORWRRUA>? MlzCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEKzNVcoRjJ{OUSyN|E4RC:jPh?=
C41 DE3 NF\rfplHfW6ldHnvckBie3OjeR?= MUK2NEB2VQ>? NGn6cXRKdmGldHn2ZZRqd25ib3[gR3lROkJ4IDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgR|QyKESHMzDj[YxteyCjc4Pld5Nm\CCjczDtc4Rq\mmnZDDhdI8uWDR3MDCyRlYh[WSmdXP0JIZwem2jdHnvckBifCB4MDD1UUBjgSCHU1mtUGMuVVNiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhVkGGUFigZY5lKEeVSFXF M2rZfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN|E4Lz5{Mkm0NlMyPzxxYU6=
C41 DE3 NIHO[FRHfW6ldHnvckBie3OjeR?= MoPkNVAxKHWP MYOzNEBucW6| NXfscHEzUW6jY4TpeoF1cW:wIH;mJGN[WDKENjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGM1OSCGRUOgZ4VtdHNiYYPz[ZN{\WRiYYOgeJJ6eHSrYzDw[ZB1cWSnIEG4PGZJYVGGUVWxPVQhf2m2aDDzbZRmKG:oIHHk[JVkfCCob4LtZZRqd25ibH;jZYxqgmWmIITvJGdtdjF7MzDheEAyODBidV2gZYZ1\XJiM{CgcYlveyCkeTDMR{1OWy:PUzDhcoFtgXOrczDpckBxemW|ZX7j[UBw\iCQQVTQTC=> NUHKUIFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK5OFI{OTdpPkKyPVQzOzF5PD;hQi=>
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MDA-MB-231 M4PUbGN6fG:2b4jpZ4l1gSCjc4PhfS=> MVW0PEBpenN? MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTJwNEJOwG0> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{MkK4NUc,OjNyMkKyPFE9N2F-
MCF7 NWDyeYxlS3m2b4TvfIlkcXS7IHHzd4F6 MYK0PEBpenN? MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCLQ{WwQVEvQDMQvF2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF{M{CxO{c,OjNzMkOwNVc9N2F-
DU145 M2O4NGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NVXpdmdqPDhiaILz MnLmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTVwNd88US=> MmXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OEewOVcoRjJ|Mki3NFU4RC:jPh?=
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MCF7 NUfSZXdOSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NV;Zc5hxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzMEBKSzVyPUGzMlfPxE1? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF3NUS2PUc,OjdzNUW0Olk9N2F-
MDA-MB-231 M2PlfmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NG\oWmRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNuIFnDOVA:OTdwOd88US=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF3NUS2PUc,OjdzNUW0Olk9N2F-
MCF7 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MWi0PEBpenN? MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVUvO87:TR?= NHjmPWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G3Olk1PCd-MkexO|Y6PDR:L3G+
Ishikawa M17PTmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXLwbmViPDhiaILz NFPjeZdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME22{txO NGryc489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G3Olk1PCd-MkexO|Y6PDR:L3G+
MDA-MB-231 NW[1WHZrSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVi0PEBpenN? NHTNT5JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE{NjoQvF2= NEC3bZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G3Olk1PCd-MkexO|Y6PDR:L3G+
MCF7 MYTDfZRwfG:6aXPpeJkh[XO|YYm= MnzUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{BjgSC{ZYPhfpVzcW5ibXnjdo9xdGG2ZTDhd5NigSxiSVO1NF03Njl7zszN NFrITZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{KyPFE2QSd-MkeyNlgyPTl:L3G+
MCF7 M4LzNmN6fG:2b4jpZ4l1gSCjc4PhfS=> NEj2bZJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDifUBz\XOjeoXybY4hdWmlcn;wcIF1\SCjc4PhfUwhUUN3ME2xPU4{O87:TR?= NFjndlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{OxNVg6PCd-MkezNVE5QTR:L3G+
MCF7 NFjydIxCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXux[mRsPzJiaILz NY\kUlNUSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGWi2yb4PpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDt[YF{fXKnZDDh[pRmeiB5MjDodpMh[nliQXzhcYFzSmy3ZTDhd5NigSxiSVO1NF01NjIQvF2= NFfxV5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{SwO|A{OCd-Mke0NFcxOzB:L3G+
insect cells NGf6bZZHfW6ldHnvckBie3OjeR?= MnvlSIl{eGyjY3Xt[Y51KG:oIF\seY9zdW:wZTDFV|IhT3KnZX6g[pJwdSCqdX3hckBz\WOxbXLpcoFvfCCodXzsJIxmdme2aDDFVoFteGijIHX4dJJme3OnZDDpckBqdnOnY4SgZ4VtdHNibXXhd5Vz\WRidYCgeI8hPCCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OD1yLkKyNu69VQ>? NX\jVVhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OFc{PzVpPkK3OlQ4Ozd3PD;hQi=>
SH-SY5Y NYPFfIN1TnWwY4Tpc44h[XO|YYm= MoHqNUBpeg>? NWDkVI9jUW6qaXLpeIlwdiCxZjDQT2MhcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDNRU1{fGmvdXzheIVlKE2DUlPLV{BxcG:|cHjvdplt[XSrb36gdJJmcW6ldXLheIVlKG[xcjCxJIhzKG[xbHzve4VlKGK7IGDNRU1{fGmvdXzheIlwdiCob4KgNVUhdWmwczDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNuIFnDOVA:OjEQvF2= NVrJPJZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OFc{PzVpPkK3OlQ4Ozd3PD;hQi=>
FADU NV\zbIZpSW62aXPhcoNmeiCjc4PhfS=> NGn0TGs1QCCqcoO= NVH0c|Y{SW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGZCTFViY3XscJMhdWWjc4Xy[YQh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME21MlM6|ryP M2izclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkexOFk4Lz5{N{[3NVQ6PzxxYU6=
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DU145 NHPqSYJCdnSrY3HuZ4VzKGG|c3H5 NInleIw1QCCqcoO= MYDBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OD1zOD6wO:69VQ>? NXTG[3hzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2O|E1QTdpPkK3OlcyPDl5PD;hQi=>
MCF7 MXfBcpRq[2GwY3XyJIF{e2G7 M1n2SlQ5KGi{cx?= MYfBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRWKtdI9{cXSrdnWgUWNHPyClZXzsd{Bu\WG|dYLl[EBi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUG4MlE5|ryP Ml32QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4N{G0PVcoRjJ5NkexOFk4RC:jPh?=
MCF7 NXzteYFOS3m2b4TvfIlkcXS7IHHzd4F6 MlG3NVIxKGi{cx?= NEfUSHNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDF{MDDodpMh[nliTWTUJIF{e2G7LDDHTVUxRTZwON88US=> NHz0[WM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG1NlQzQSd-MkixOVI1Ojl:L3G+
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SKBR3 MkGzR5l1d3SxeHnjbZR6KGG|c3H5 NGrDbowyOjBiaILz NYPi[pN2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiMUKwJIhzeyCkeTDNWHQh[XO|YYmsJGdKPTB;MUKuOe69VQ>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF3MkSyPUc,OjhzNUK0Nlk9N2F-
MDA-MB-231 MlzER5l1d3SxeHnjbZR6KGG|c3H5 MormNVIxKGi{cx?= NIjlVIFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFEzOCCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVE2NjIQvF2= NVO4VIlWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixOVI1OjlpPkK4NVUzPDJ7PD;hQi=>
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insect cells Mnr5SpVv[3Srb36gZZN{[Xl? NHzwdZlFcXOybHHj[Y1mdnRib3[g[ox2d3Knc3PlbY4udGGkZXzl[EBme3S{YXTpc4whMG[udX;yc41wdmVrIH\yc40hcHWvYX6gdoVkd22kaX7hcpQh\nWubD3s[Y5ofGhidX70ZYdo\WRiZYP0do9o\W5icnXj[ZB1d3JiYnX0ZUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[XO|YYmsJGlEPTB;MD6xPFjPxE1? M1vNdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEK2PVMyLz5{OESyOlk{OTxxYU6=
Ishikawa cells NYL3XWszTnWwY4Tpc44h[XO|YYm= MmDLO|IhcHK| Mn7HRY51[WexbnnzeEBi[3Srdnn0fUBifCCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZU9j\XSjIHnuJIh2dWGwIFnzbIls[XejIHPlcIx{KGGodHXyJFczKGi{czDifUBidGujbHnu[UBxcG:|cHjheIF{\SCjc4PhfUwhUUN3ME2wMlI5|ryP NH31cIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESyOlk{OSd-Mki0NlY6OzF:L3G+
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MCF7 M1jWTGN6fG:2b4jpZ4l1gSCjc4PhfS=> MUW1JIRigXN? MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA2KGSjeYOgZpkhW1KEIHHzd4F6NCCLQ{WwQVQvPM7:TR?= M2izRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEK2PVMyLz5{OESyOlk{OTxxYU6=
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insect cells NH7L[JBHfW6ldHnvckBie3OjeR?= MX6yJIhzew>? MkS5SIl{eGyjY3Xt[Y51KG:oIH\seY9z\XOlZX70JIV{fHKxZ3XuJIxq\2GwZDDmdo9uKHKnY3;tZolv[W62IHj1cYFvKEWUYXzwbIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BqdmO3YnH0[YQh\m:{IEKgbJJ{KGK7IIDvcIFzcXqjdHnvckwhUUN3ME2yMlY4|ryP NHvN[2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
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Ishikawa cells M2fIT2N6fG:2b4jpZ4l1gSCjc4PhfS=> NELVPVI1QCCqcoO= NHvaVXhEgXSxdH;4bYNqfHliaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlAvPjYQvF2= NYj3SmNnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
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MCF7 M3;BOmN6fG:2b4jpZ4l1gSCjc4PhfS=> NGLRZW4zPCCqcoO= MonLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUB2eCC2bzCxNFAhfU1iYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigQ>? NID2cVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC0OVE{PSd-MkmwOFUyOzV:L3G+
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U2O2 NIG2NpdHfW6ldHnvckBie3OjeR?= NGf2W5YyKHWP MYO0JIhzew>? M3TIRWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iR1\QMYZ2e2WmIFXSZYxxcGFiTFLEJEg{ODFidH:gOVU{KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gbJVu[W5iVULPNkBk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kAyP2KndHGt[ZN1emGmaX;sMYlv\HWlZXSgeJJidnOlcnnweIlwdiCjdDCxJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhPCCqcoOg[o9tdG:5ZXSgZpkhTTJiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOjBiaILzJIJ6 MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB2NUGzOUc,OjlyNEWxN|U9N2F-
MCF7 MnXDRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MofMOFghcHK| NVLEfFl7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;OD6xNu69VQ>? Ml\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyNUSzOVkoRjJ7MEW0N|U6RC:jPh?=
MDA-MB-231 MluwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1fZRlQ5KGi{cx?= NHT5NnhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF06Njl4zszN MmDnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyNUSzOVkoRjJ7MEW0N|U6RC:jPh?=
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MCF7 Mn7WR4VtdCCleXPs[UBie3OjeR?= MX2xNEB2VQ>? MnTwNlQhfG9iNEigbJJ{ MWnD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOC:JMTDwbIF{\SCjdDCxNEB2VSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=> M1jSfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MEW0N|U6Lz5{OUC1OFM2QTxxYU6=
MCF7 MlXjR5l1d3O2YYTpZ{Bi[3Srdnn0fUBie3OjeR?= M{TQTWN6fG:|dHH0bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzMEBKSzVyPUKuO:69VQ>? MlzFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MEezN|UoRjJ7MkC3N|M2RC:jPh?=
MCF7 NWW3PJcyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXfnS2d7PzJiaILz M2fX[Gdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME21MlPPxE1? M4H1dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkKwO|g6Lz5{OUKyNFc5QTxxYU6=
MCF7 NUL2T5Q1SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NETwTHY1QCCqcoO= M1;uOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF7LkZOwG0> M{fFU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEiyPVQ1Lz5{OUS4Nlk1PDxxYU6=
Ishikawa cells Mmf6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MoS1QlQxKHWP M36zeFQ5KGi{cx?= M2rLOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSYPobYtif2FiY3XscJMh[XRiPkSwJJVOKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0OEc,Ojl2OEK5OFQ9N2F-
MCF7 NYny[lIyTnWwY4Tpc44h[XO|YYm= NYjmbm5OOSC3TR?= MXXBcpRi\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\XO2cnHkbY9tNWmwZIXj[YQhWGeUIH3SUmEh\XiycnXzd4lwdiCjdDCxJJVOKGK7IGPZRnIhT3KnZX6g[JlmKGKjc3XkJHJVNVCFUjDhcoFtgXOrcx?= MmjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OEK5OFQoRjJ7NEiyPVQ1RC:jPh?=
MCF7 NEDSNZhHfW6ldHnvckBie3OjeR?= MWGyOEBpenN? M1[3[Glv\HWldHnvckBw\iC|ZXzlZ5RqfmViZYP0do9o\W5icnXj[ZB1d3JiYXzwbIEh\GWpcnHkZZRqd25iaX6gbJVu[W5iTVPGO{Bk\WyuczDoZZJjd3KrbnegWGsuTVKHLVz1Z{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[ZN1emGmaX;sMYlv\HWlZXSgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MT62NFXPxE1? NUPU[oN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlI4OzdpPkK5OVYzPzN5PD;hQi=>
MCF7 M4LMbWZ2dmO2aX;uJIF{e2G7 M4DLO|YxKG2pL3vn NF;5[2U1QCCmYYnz MmflSI94dnKnZ4XsZZRqd25ib3[gdJJw\2W|dHXyc45mKHKnY3XweI9zKG2UTlGgcIV3\WxiaX6gUm9FKFOFSVSg[4FudWFibX;1d4UhgGWwb3fyZYZ1\WRid3n0bEBpfW2jbjDNR2Y4KGOnbHzzJIF1KDZyIH3nM4toNCCybzC1JIRigXNicHXyJJdm\WtiZn;yJFQ5KGSjeYOgdI9{fCC2dX3vdkBqdXCuYX70ZZRqd25ibXXhd5Vz\WRiNzDodpMheG:|dDDsZZN1KGSxc3WgZpkhWlRvUFPSJI1mfGixZB?= NXrxSXFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlI4OzdpPkK5OVYzPzN5PD;hQi=>
insect cells MnnwSpVv[3Srb36gZZN{[Xl? NHjWOJIzKGi{cx?= NIOzN3BFcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIH\1cIwhdGWwZ4ToJIh2dWGwIFXSMYFteGijIHX4dJJme3OnZDDpckBqdnOnY4SgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OD1zLkSy{txO NUexcmpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFczOjFpPkK5OVg4OjJzPD;hQi=>
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Ishikawa cells MlXoRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MnLmOFghcHK| NXSwOVQ5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI3NjV{zszN NF\LSVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4O|IzOSd-Mkm1PFczOjF:L3G+
MRC5 SV2 NUK1b|JJS3m2b4TvfIlkcXS7IHHzd4F6 MnfMO|IhcHK| MnvjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXJEPSCVVkKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWtZoF{\WRiZnz1c5JqdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUGwMlLPxE1? NIDONHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[wOFU1OSd-Mkm2NFQ2PDF:L3G+
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PANC1 NEDxRZhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkG4S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yOs7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZyNUi2OUc,OzF4MEW4OlU9N2F-
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RAW264.7 NGTDO2hEgXSxdH;4bYNqfHliYYPzZZk> NXXWOmJCOjRiaILz MnizR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEK0JIhzeyCkeTDu[ZV1emGuIILl[EBlgWViYnHz[YQh[XO|YYmsJGNEPTB;MUGuNe69VQ>? NXjYSZhLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3NFM5OThpPkOxO|A{QDF6PD;hQi=>
Assay
Methods Test Index PMID
Western blot p-AKT / AKT ; ER-α66 / ER-α36 / HER2 / EGFR ; p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; CD36 / p-ERK / ERK 30759864 25203051 27020861 30573731
Immunofluorescence CD36 30573731
In vivo

Tamoxifen administration to rapidly growing, estradiol-stimulated MCF-7 xenografts results in a dose-dependent retardation or cessation of tumor growth by significantly decreasing tumor cell proliferation in tumor. Tamoxifen treatment results in a slowing of tumor growth (tumor doubling time, 12 days), a significant increase in tumor potential doubling time (Tpot) (6.6 days), and a decrease in labeling index (%LI) (to 8%) by 23 days posttreatment, compared with untreated mice which shows a volume doubling time of 5 days, a Tpot of 2.3 days, and a %LI of 23%. [5] Tamoxifen has not only antiestrogenic but also estrogenic properties depending on the species, tissue, and gene. Tamoxifen displays favorable effects on bone and serum lipid concentrations and stimulation endometrium. [1]

Protocol (from reference)

Kinase Assay:

[3]

  • Competitive binding assays:

    Cells are harvested from 150-sq cm T-flasks, and cytosol is prepared at a protein concentration of approximately 2 mg/mL in phosphate buffer. Aliquots of this 180,000 ×g supernatant are then incubated with various concentrations of Tamoxifen and 2.5 nM [3H]estradiol for 16 hr at 0-4 ℃. The free steroids are absorbed by dextran-charcoal [l0 μL of 0.5% Dextran C-5% Norite A in TE buffer] for 1 hr at 0 ℃, and aliquots are counted after centrifugation at 800 ×g, 30 min. The relative binding ability of each competitor is taken as the ratio of the concentration of radioinert estradiol/competitor required to inhibit one-half of the specific [3H]estradiol binding, with the affinity of estradiol set at 100%.

Cell Research:

[3]

  • Cell lines: Human breast cancer cells MCF-7
  • Concentrations: ~1 μM
  • Incubation Time: 10 days
  • Method:

    MCF-7 cells are seeded into T-25 flasks (1.5×105 cells/flask) and grown for 2 days in the MEM supplemented with 10 mM HEPES buffer, gentamicin (50 μg/mL), penicillin (100 units/mL), streptomycin (0.1 mg/mL), bovine insulin (6 ng/mL), hydrocortisone (3.75 ng/mL), and 5% calf serum that has been treated with dextran-coated charcoal for 45 min at 55 ℃ to remove endogenous hormones. The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen. At the end of incubation, cell numbers are counted.

Animal Research:

[5]

  • Animal Models: Human breast carcinoma xenografts MCF-7
  • Dosages: 2 cm Tamoxifen capsules
  • Administration: Subcutaneous implantation

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.

30 mg/mL

Chemical Information

Molecular Weight 563.64
Formula

C26H29NO.C6H8O7

CAS No. 54965-24-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3.C(C(=O)O)C(CC(=O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05156892 Not yet recruiting Drug: SUBA-itraconazole|Drug: Tamoxifen Ovarian Cancer Anthony Joshua FRACP|Royal Prince Alfred Hospital Sydney Australia|Concord Hospital|Prince of Wales Hospital Sydney|St Vincent''s Hospital Sydney February 1 2022 Phase 1
NCT05133674 Not yet recruiting Drug: Tamoxifen 20 mg Breast Cancer|Breast Carcinoma|Breast Tumors|Cancer of Breast|Malignant Neoplasm of Breast Karolinska University Hospital January 10 2022 Phase 2
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester December 1 2021 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?

Answer:
The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

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