Tamoxifen (ICI 46474) Citrate

For research use only.

Catalog No.S1972

14 publications

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.

Selleck's Tamoxifen (ICI 46474) Citrate has been cited by 14 publications

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  • Effects of DPN, LY500307, Raloxifene and Tamoxifen on cell viability in BSO-treated FRDA fibroblasts. BSO concentration was 1 mM and all steroid concentrations were 100 nM. Depicted are mean ± SD for n= 8 per group. * indicated p<0.05 versus BSO alone-treated cells.

    University of North Texas Health Science Center, 2014.. Tamoxifen (ICI 46474) Citrate purchased from Selleck.

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.
Targets
ER [1]
(Cell-free assay)
In vitro

Tamoxifen displays antitumor effect due to its antiestrogenic activity (ER). Values for the apparent affinity of Tamoxifen for the ER range between 30 and 0.01% of that obtained for estradiol, dependent on different ER source (species), protein concentration and condition used for assay. Binding of Tamoxifen to ER further leads to inhibition expression of estrogen-regulated genes, including growth factors and angiogenic factors secreted by the tumor that may stimulate growth by autocrine or paracrine mechanisms. Tamoxifen also directly induces programmed cell death. [1] Tamoxifen produces an inhibitory effect on MCF-7 cell [3H]thymidine incorporation and DNA polymerase activity as well as causing a reduction in DNA content of cultures and cell numbers. This inhibitory effect of Tamoxifen on MCF-7 cell growth can be readily reversed by addition of estradiol to the culture medium. 2 and 6 μM Tamoxifen reduces the proportion of cells in S phase and increases the number of cells in G1. At 10 μM, Tamoxifen causes cell death within 48 hr. [2] Tamoxifen inhibits MCF-7 growth with IC50 of ~10 nM after 10 days treatment. Tamoxifen inhibits plasminogen activator activity of MCF-7, and suppresses estradiol-stimulation of plasminogen activator activity. Tamoxifen also evokes minimal increases in cellular progesterone receptor levels. [3] Tamoxifen is able to inhibit the growth of prostate cancer cell PC3, PC3-M, and DU145 with IC50 ranged from 5.5-10 μM, which is related to its inhibition of protein kinase C and induction of p21(waf1/cip1). [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells MXjDfZRwfG:6aXRCpIF{e2G7 NH7lfmgyQCCq M4rvbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgNVghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE4zKM7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
human MDA-MB-231 cells NY\QfZRIS3m2b4TvfIlkyqCjc4PhfS=> NWTTd|l3OThiaB?= M{\MTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiMUigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj6zJO69VQ>? NIDTOVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzNlAzPCd-MUm5N|IxOjR:L3G+
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ER-positive MCF7 MnvBRY51cWW|dILv[4VvcWNiYYPzZZk> NGrYT2QzOCCwTR?= MkLLOFghcHK| NXW2PXpVSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gSXIueG:|aYTpeoUhVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKDF5YnX0ZUBme3S{YXTpc4wucW6mdXPl[EB{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCjY4Tpeol1gSCjdDCyNEBvVSCjZoTldkA1QCCqcoOgZpkheGixc4DoZU1tcWeqdDDy[ZBwenSncjDjbIVucWy3bXnu[ZNk\W6lZTDhd5NigQ>? Mn\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEC4OVkoRjJzOECwPFU6RC:jPh?=
HEK293 MorzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGLpfFg1QCCqcoO= M1HEO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUFOwG0> MkGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MCF7 NVH5O4M{SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mm\NOFghcHK| NYL1VZdVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5Ih[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNk41QM7:TR?= NIjifmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3NVgyOid-MkG4O|E5OTJ:L3G+
MDA-MB-231 MkizRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NVfyVVJVPDhiaILz M1zVfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF6LkpOwG0> NXTrU5NwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O|E5OTJpPkKxPFcyQDF{PD;hQi=>
Ishikawa NXKwXHJzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NES3b|k1QCCqcoO= MlXNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BmgHC{ZYPzbY5oKGW|dILv[4VvKHKnY3XweI9zKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlIvPc7:TR?= M4[yWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEexPFEzLz5{MUi3NVgyOjxxYU6=
A673 NG\XOWhyUFSVIHHzd4F6 MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MkPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2N{KwOFUoRjJ{NEeyNFQ2RC:jPh?=
HEK293 MWXGeY5kfGmxbjDhd5NigQ>? NUnZcmh[OS53IHjydy=> MXTEbZNxdGGlZX3lcpQhd2ZiW{PIYWxUTCCocn;tJIh2dWGwIEXIWFYhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bi\nSncjCxMlUhcHK|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaU2xMlA1Oc7:TR?= NEjKUpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWzO|E2Oyd-MkK1N|cyPTN:L3G+
AH109 MYfGeY5kfGmxbjDhd5NigQ>? NYjCfmE1OjRiaILz NVW1Uo9GSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoJmfGFicnXj[ZB1d3JiTFLEJIV5eHKnc4Pl[EBqdiC7ZXHzeEBCUDFyOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiSVO1NF0yNjZ4zszN MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
AH109 M2rIdWZ2dmO2aX;uJIF{e2G7 NX62UlY3OjRiaILz NYD2XHNSSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIILlZ4VxfG:{IFzCSEBmgHC{ZYPz[YQhcW5ieXXhd5QhSUhzMEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTldoFkfGmxbjD3bZRpKFOUQ{GgZYZ1\XJiMkSgbJJ{KGK7IHHsdIhiNWejbHHjeI9{cWSjc3WgZZN{[XluIFnDOVA:Oi53NN88US=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
MCF7 M3XxSGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWr0bGk4OjRiaILz M1nRSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvPTUQvF2= NWq3T|dHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
DLD1 MWHDfZRwfG:6aXPpeJkh[XO|YYm= NYDPcJJ3QTZiaILz MnPxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSGxFOSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUSuO|jPxE1? Ml\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
MCF7 NWOzTVRDS3m2b4TvfIlkcXS7IHHzd4F6 MlPhPVYhcHK| MlvaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUeuNe69VQ>? NHHnbXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
NIH/3T3 M4HhcmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{LIZVk3KGi{cx?= M4\JdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9|VEOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjJ4zszN NUfqZlZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
518A2 MmXJR5l1d3SxeHnjbZR6KGG|c3H5 NXXMUnN6QTZiaILz MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUeuOlLPxE1? M4PaSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
A2780 M{XqZWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYS5UYJIQTZiaILz NF:5Z49EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvPzgQvF2= NYnPSFN6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
Lipo NI\CPFJEgXSxdH;4bYNqfHliYYPzZZk> NE\GSpI6PiCqcoO= MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD62OO69VQ>? NIfWenA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
A253 MnHjR5l1d3SxeHnjbZR6KGG|c3H5 NH3re|E6PiCqcoO= MojJR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI2OyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUiuPVLPxE1? MoTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
A549 NIrhemJEgXSxdH;4bYNqfHliYYPzZZk> MnTlPVYhcHK| M4HtRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME25MlY3|ryP MoG2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
850C M2nhOWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2DMUFk3KGi{cx?= MoPLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gPFUxSyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUGxMlA6|ryP NX3ZVlJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
KIF5 M3zCcnRwgGmlaYT5JIF{e2G7 MYqyOEBpenN? MorHWI95cWOrdImgZYdicW6|dDDoeY1idiCNSV[1JINmdGy|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEOnbHzUbZRmeiCEbIXlJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI{Njd3zszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd5NUS3OEc,OjJ5N{W0O|Q9N2F-
MCF7 MlfCR5l1d3SxeHnjbZR6KGG|c3H5 NWnPXVNkPzJiaILz MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QyKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIEKwKUBHSlNuIFnDOVA:QC54zszN NFTCTGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
HL60 NYPaR5dYS3m2b4TvfIlkcXS7IHHzd4F6 NISzRXc4OiCqcoO= MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QyKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIEKwKUBHSlNuIFnDOVA:OTRwM988US=> M4DBSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUCxPFk2Lz5{MkmwNVg6PTxxYU6=
C41 DE3 M1TkbWZ2dmO2aX;uJIF{e2G7 MlTqOlAhfU1? M3jjOVExKG2rboO= NV;kc|JXUW6jY4TpeoF1cW:wIH;mJGN[WDKENjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGM1OSCGRUOgZ4VtdHNiYYPz[ZN{\WRiYYOgcY9lcW[rZXSgZZBwNVB2NUCgNmI3KGGmZIXjeEBnd3KvYYTpc44h[XRiNkCgeW0h[W[2ZYKgNVAhdWmwczDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEh? NF;ncI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm0NlMyPyd-MkK5OFI{OTd:L3G+
C41 DE3 MYPGeY5kfGmxbjDhd5NigQ>? MmfpOlAhfU1? MVfJcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyCvb3Tp[olm\CCjcH:tVFQ2OCB{Qk[gZYRlfWO2IH\vdo1ifGmxbjDheEA3OCC3TTDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEhiYX7kJGdUUEWH M4q3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN|E4Lz5{Mkm0NlMyPzxxYU6=
C41 DE3 NVTOZXBqTnWwY4Tpc44h[XO|YYm= MlPmNVAxKHWP M1HjPVMxKG2rboO= MUDJcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyC2conweIlkKHCncITp[IUhOTh6RljZVWRSTTF7NDD3bZRpKHOrdHWgc4Yh[WSmdXP0JIZwem2jdHnvckBtd2OjbHn6[YQhfG9iR3zuNVk{KGG2IEGwNEB2VSCjZoTldkA{OCCvaX7zJIJ6KEyFLV3TM21UKGGwYXz5d4l{KGmwIIDy[ZNmdmOnIH;mJG5CTFCK MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl2MkOxO{c,OjJ7NEKzNVc9N2F-
MCF7 MnjOR5l1d3SxeHnjbZR6KGG|c3H5 MnrkOFghcHK| M2n2bWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNU41PM7:TR?= NVfRWlA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlIzQDFpPkKzNFIzOjhzPD;hQi=>
MDA-MB-231 NH;5bZREgXSxdH;4bYNqfHliYYPzZZk> MYS0PEBpenN? M2D5TmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj60Ne69VQ>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{MkK4NUc,OjNyMkKyPFE9N2F-
MCF7 M1viPWN6fG:2b4jpZ4l1gSCjc4PhfS=> MoHVOFghcHK| NEn1UG5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR|UxRTFwOENOwG0> M4rOeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUKzNFE4Lz5{M{GyN|AyPzxxYU6=
DU145 MX3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M330dlQ5KGi{cx?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESXMUS1JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS53zszN NF\rU|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O|A2Pyd-MkOyPFcxPTd:L3G+
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Ishikawa cells M2TKfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2q2fFQ5KGi{cx?= NVLJepk5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE3NjR5zszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4MEixPUc,Ojh2NkC4NVk9N2F-
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HeLa MlrqSpVv[3Srb36gZZN{[Xl? NH\lS4QzPCCqcoO= NFr0eINCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IH\1cIwhdGWwZ4ToJGVT[WyyaHGgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDFVmUu\HKrdnXuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjN2Md88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh6MkWwNkc,Ojh6OEK1NFI9N2F-
HeLa M1fRPGZ2dmO2aX;uJIF{e2G7 M1fS[FI1KGi{cx?= M33RXWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiZoXscEBt\W6pdHigSXJj\XSjIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliRWLFMYRzcX[nbjDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NU42O87:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh6MkWwNkc,Ojh6OEK1NFI9N2F-
MCF7 NIXkbHBEgXSxdH;4bYNqfHliYYPzZZk> M4mzUFczKGi{cx?= MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDy[ZNignW{aX6gZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxRTdwOUVOwG0> M4LsN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUK3O|k2Lz5{OEmyO|c6PTxxYU6=
insect cells MoTDSpVv[3Srb36gZZN{[Xl? NVnkWW1rOiCqcoO= NFewXm5FcXOybHHj[Y1mdnRib3[g[ox2d3Knc3PlcpQh\XO2cn;n[Y4hdGmpYX7kJIZzd21icnXjc41jcW6jboSgbJVu[W5iRWLhcJBp[SCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{KGmwY4XiZZRm\CCob4KgNkBpenNiYomgdI9t[XKrenH0bY9vNCCLQ{WwQVIvPjgQvF2= NFLHSW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
MCF7 Mm\DR5l1d3SxeHnjbZR6KGG|c3H5 NEnxdpo1QCCqcoO= NHXrcmtEgXSxdH;4bYNqfHliaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zND6zOe69VQ>? NX7KR4ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
Ishikawa cells NXTFSWtLS3m2b4TvfIlkcXS7IHHzd4F6 MWC0PEBpenN? MVrDfZRwfG:6aXPpeJkhcW5iaIXtZY4hUXOqaXvhe4Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkCuOlXPxE1? NEjMV|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
MCF7 MW\GeY5kfGmxbjDhd5NigQ>? MXOxNEBvVQ>? M37u[|I1KGi{cx?= NV\LRnhxUW6qaXLpeIlwdiCxZjDFVoFteGijIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIEGwJI5OKGW|dILv[4VvNWmwZIXj[YQheHKxZ3XzeIVzd26nIILlZ4VxfG:{IHX4dJJme3Orb36gZZQhOSC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2MkGxN{c,Ojh7NEKxNVM9N2F-
MCF7 MYrDfZRwfG:6aXPpeJkh[XO|YYm= M3u5TlI1KGi{cx?= NXnVToNtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSC3cDD0c{AyODBidV2gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= NEXKNVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC0OVE{PSd-MkmwOFUyOzV:L3G+
MCF7 MX3GeY5kfGmxbjDhd5NigQ>? NWfOT2o{OSC3TR?= NF;zZnA1KGi{cx?= NIrSRWhKdmirYnn0bY9vKG:oIFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kAyP2KndHGt[ZN1emGmaX;sMY1m\GmjdHXkJJBUOiCvUl7BJIV5eHKnc4Ppc44h[XRiMTD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JFE4[mW2YT3ld5Rz[WSrb3ygZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMU[gbJJ{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KgZY5idHm|aYO= M2S5NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MES1NVM2Lz5{OUC0OVE{PTxxYU6=
U2O2 NVPoRZJ5TnWwY4Tpc44h[XO|YYm= MV2xJJVO NU[4UZhbPCCqcoO= NYKxRlE2UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDHSnAu\nW|ZXSgSXJidHCqYTDMRmQhMDNyMTD0c{A2PTNicnXzbYR2\XNrIHX4dJJme3OnZDDpckBpfW2jbjDVNm8zKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKDF5YnX0ZU1me3S{YXTpc4wucW6mdXPl[EB1emGwc3PybZB1cW:wIHH0JFEhfU1icILlbY5kfWKjdHXkJIZweiB2IHjyd{Bnd2yub4fl[EBjgSCHMjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{MDDodpMh[nl? MnHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyNEWxN|UoRjJ7MES1NVM2RC:jPh?=
MCF7 NYXIZlB6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUT1W4h3PDhiaILz NWj2SmxVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;OD6xNu69VQ>? NGfzT5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
MDA-MB-231 NIP3bWxCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{WzRVQ5KGi{cx?= NYPzfZpESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QS57Nt88US=> NWLQNWhWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwOVQ{PTlpPkK5NFU1OzV7PD;hQi=>
HEK293 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MW[0PEBpenN? M333fGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTGVMOjl|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlAvQTQQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB3NEO1PUc,OjlyNUSzOVk9N2F-
MCF7 MkjDR4VtdCCleXPs[UBie3OjeR?= NWP6PXFpOTBidV2= M{fad|I1KHSxIES4JIhzew>? MXjD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOC:JMTDwbIF{\SCjdDCxNEB2VSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=> NYPLb2hmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwOVQ{PTlpPkK5NFU1OzV7PD;hQi=>
MCF7 MWLDfZRwe3SjdHnjJIFkfGm4aYT5JIF{e2G7 Mlf5R5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTB;Mj63{txO NWXhfGxmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNFc{OzVpPkK5NlA4OzN3PD;hQi=>
MCF7 NGrJUGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWq0UlJ5PzJiaILz MXXHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NT6z{txO MnnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MkC3PFkoRjJ7MkKwO|g6RC:jPh?=
MCF7 NH7uUo1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3PUS|Q5KGi{cx?= MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQS53zszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0OEc,Ojl2OEK5OFQ9N2F-
Ishikawa cells NU\Yeo1vSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYO+OFAhfU1? Mnu4OFghcHK| NXvGUFh6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjdDC+OFAhfU1iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>? M2fmTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEiyPVQ1Lz5{OUS4Nlk1PDxxYU6=
MCF7 NYHIVoVyTnWwY4Tpc44h[XO|YYm= NHXMOJQyKHWP MnXJRY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGW|dILh[IlwdC2rbnT1Z4VlKFCpUjDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCkeTDTXWJTKEe{ZXXuJIR6\SCkYYPl[EBTXC2SQ2KgZY5idHm|aYO= NHvaTms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS4Nlk1PCd-Mkm0PFI6PDR:L3G+
MCF7 M2nTdWZ2dmO2aX;uJIF{e2G7 NYDZSIs3OjRiaILz NFzJTGFKdmS3Y4Tpc44hd2Zic3Xs[YN1cX[nIHXzeJJw\2WwIILlZ4VxfG:{IHHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgbIFz[m:{aX7nJHRMNUWURT3MeYMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIV{fHKjZHnvcE1qdmS3Y3XkJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUGuOlA2|ryP NHywUnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2Nlc{Pyd-Mkm1OlI4Ozd:L3G+
MCF7 MoD5SpVv[3Srb36gZZN{[Xl? MUi2NEBu\y:tZx?= M{nWRlQ5KGSjeYO= MVXEc5dvemWpdXzheIlwdiCxZjDwdo9o\XO2ZYLvcoUhemWlZYD0c5IhdVKQQTDs[ZZmdCCrbjDOU2QhW0OLRDDnZY1u[SCvb4Xz[UB5\W6xZ4Lh[pRm\CC5aYToJIh2dWGwIF3DSlch[2WubIOgZZQhPjBibXevb4ctKHCxIEWg[IF6eyCyZYKge4VmcyCob4KgOFgh\GG7czDwc5N1KHS3bX;yJIlueGyjboTheIlwdiCvZXHzeZJm\CB5IHjyd{Bxd3O2IHzhd5Qh\G:|ZTDifUBTXC2SQ2KgcYV1cG:m NHXLS5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2Nlc{Pyd-Mkm1OlI4Ozd:L3G+
insect cells MUnGeY5kfGmxbjDhd5NigQ>? M1T5XlIhcHK| NXjNNIp2TGm|cHzhZ4Vu\W62IH;mJGZtfW:{bX;u[UBGWzJiR4Ll[Y4h\nKxbTDmeYxtKGynbnf0bEBpfW2jbjDFVk1idHCqYTDlfJBz\XO|ZXSgbY4hcW6|ZXP0JINmdGy|IHHmeIVzKDJiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[XO|YYmsJGlEPTB;MT60Nu69VQ>? M2DGfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi3NlIyLz5{OUW4O|IzOTxxYU6=
MCF7 NVvtZ4k6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MmP5OFghcHK| NHK2dlJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zNT60Ou69VQ>? M{\kb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi3NlIyLz5{OUW4O|IzOTxxYU6=
Ishikawa cells MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MV:0PEBpenN? MkjERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUK2MlUz|ryP NFTCb409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4O|IzOSd-Mkm1PFczOjF:L3G+
MRC5 SV2 NHTSWoNEgXSxdH;4bYNqfHliYYPzZZk> MVS3NkBpenN? MoHFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXJEPSCVVkKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWtZoF{\WRiZnz1c5JqdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUGwMlLPxE1? M2X5XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkC0OVQyLz5{OU[wOFU1OTxxYU6=
MCF7 NH:0VINEgXSxdH;4bYNqfHliYYPzZZk> M1zzT|QhcHK| M2ficGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMheHKnaX7jeYJifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBqdmO3YnH0bY9vKGmwIHPvcZBwfW6mIH\y[YUhdWWmaXGg[o9zKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QC54Md88US=> M3XXXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkC1PFA5Lz5{OU[wOVgxQDxxYU6=
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insect M{j4[GZ2dmO2aX;uJIF{e2G7 MUfCbY5lcW6pIHHm[olvcXS7IITvJJVvfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JIZ2dGxibHXu[5RpKEWUYXzwbIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{AxNjFidH:gNVAxODBidV2gZpkhTmy3b4Ltc45mKEWVMjDHdoVmdiCmaYPwcIFk\W2nboSgZoF{\WRiYYPzZZk> NIjHVmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC5NVkzOCd-M{CwPVE6OjB:L3G+
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Lucena 1 MlzISpVv[3Srb36gZZN{[Xl? M1LvVlEzNjVidV2= NE\lUngyKGi{ M{P3U2lvcGmkaYTpc44hd2ZiUD3ndE1u\WSrYYTl[EBTcG9vMUKzJIVn\my3eDDpckBpfW2jbjDMeYNmdmFiMTDj[YxteyCjdDCxNk42KHWPIIDy[Ylv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCUaH:tNVI{KGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFEhcHJiYomg[oxwfyCleYTvcYV1enl? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyOEc,OzB4MUOzNlQ9N2F-
Lucena 1 NH3sRZJHfW6ldHnvckBie3OjeR?= M4D4[FI2KHWP MYOxJIhz NEm1flZKdmirYnn0bY9vKG:oIGCt[5AudWWmaXH0[YQhWmixLUGyN{Bm\m[udYigbY4hcHWvYX6gUJVk\W6jIEGgZ4VtdHNiYYSgNlUhfU1icILlbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KFKqbz2xNlMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNUBpeiCkeTDmcI94KGO7dH;t[ZRzgQ>? MojKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4MUOzNlQoRjNyNkGzN|I1RC:jPh?=
HepG2 MYXDfZRwfG:6aXPpeJkh[XO|YYm= M{D3bVI1KGi{cx?= M2roT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVLPxE1? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyO{c,OzB4MUOzNlc9N2F-
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MRC5 M4HCRWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmTFNlQhcHK| M4e5NGN6fG:2b4jpZ4l1gSCrbjDoeY1idiCPUlO1JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDy[ZNignW{aX6tZoF{\WRiY4n0c5RwgGmlaYT5JIF{e2G7LDDJR|UxRTFzLkC5{txO MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODh5OUizPUc,OzB6N{m4N|k9N2F-
HEK293 NWfW[llCS3m2b4TvfIlkcXS7IHHzd4F6 M3zJOlIxKGi{cx?= MkLnR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy|IH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgSC{ZYPhfpVzcW5iZInlJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OD1{NN88US=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODl5NUWwNkc,OzB7N{W1NFI9N2F-
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MCF7 MlPhRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2nMVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2= M2W0ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUS2NVk4Lz5|MUW0OlE6PzxxYU6=
A549 MWXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2HC[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2= NV\2W4hrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OFYyQTdpPkOxOVQ3OTl5PD;hQi=>
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DU145 MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnTHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCGVUG0OUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2= NGKwWVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUW0OlE6Pyd-M{G1OFYyQTd:L3G+
MDA-MB-231 MoXZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M3myNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;MUGuNu69VQ>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV2NkG5O{c,OzF3NE[xPVc9N2F-
SiHa NHHHfJpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIHwSmRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUcUijIHPlcIx{NCCJSUWwQVAvOTMQvF2= MnTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MEW4OlUoRjNzNkC1PFY2RC:jPh?=
MDA-MB-231 M{jWSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4O5Zmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOsJGdKPTB;MD6xNu69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZyNUi2OUc,OzF4MEW4OlU9N2F-
PANC1 NYfYeWtmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmG3S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yOs7:TR?= NGi4THE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MU[wOVg3PSd-M{G2NFU5PjV:L3G+
MCF7 NV7oTYUySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1jjWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVI2|ryP MoLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MUCzO|goRjNzNkGwN|c5RC:jPh?=
M-HeLa NEjuS5ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE1vSHXMZUBk\WyuczDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVI5|ryP NHvJVY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MU[xNFM4QCd-M{G2NVA{Pzh:L3G+
Chang M160XGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDbIFv\yClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYomg[ox2d3Knc3PlcoNmKGKjc3XkJJZq[WKuZTDj[YxtKGOxdX70bY5oKG2ndHjv[EwhUUN3ME20Nk4y|ryP Mnm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MUCzO|goRjNzNkGwN|c5RC:jPh?=
MCF7 MnXHR5l1d3SxeHnjbZR6KGG|c3H5 NUTJcXh7PDhiaILz M13KPWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zOi54zszN MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZ{MEKyOUc,OzF4MkCyNlU9N2F-
RAW264.7 MWnDfZRwfG:6aXPpeJkh[XO|YYm= NETHT40zPCCqcoO= NYSzbYN5S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBv\XW2cnHsJJJm\CCmeXWgZoF{\WRiYYPzZZktKEOFNUC9NVEvOc7:TR?= M3\0[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzN{CzPFE5Lz5|MUewN|gyQDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT ; 

PubMed: 30759864     


MCF-7 cells were treated with 2 μM tamoxifen and harvested at the indicated time point to analyze the expressions of p-AKT and AKT by western blot.

ER-α66 / ER-α36 / HER2 / EGFR ; 

PubMed: 25203051     


Western blot analysis of the expression levels of ER-α66, ER-α36, HER2 and EGFR in ER-positive breast cancer MCF7 (A), T47D (B) and H3396 (C) cells treated with 1 µM of tamoxifen (TAM) for indicated time period.

p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; 

PubMed: 27020861     


B. Immunoblot of AMPK pathway activation following treatment with 30μM tamoxifen in MCF7 and C. in MDA-MB-231 cells. 

CD36 / p-ERK / ERK ; 

PubMed: 30573731     


MCF-7 cells MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h, or with 5 μM Tam for the indicated times. Expression of CD36, p-ERK1/2 and ERK1/2 was determined by western blot.

30759864 25203051 27020861 30573731
Immunofluorescence
CD36; 

PubMed: 30573731     


MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h. Expression of CD36 was determined by immunofluorescent staining . 

30573731
In vivo

Tamoxifen administration to rapidly growing, estradiol-stimulated MCF-7 xenografts results in a dose-dependent retardation or cessation of tumor growth by significantly decreasing tumor cell proliferation in tumor. Tamoxifen treatment results in a slowing of tumor growth (tumor doubling time, 12 days), a significant increase in tumor potential doubling time (Tpot) (6.6 days), and a decrease in labeling index (%LI) (to 8%) by 23 days posttreatment, compared with untreated mice which shows a volume doubling time of 5 days, a Tpot of 2.3 days, and a %LI of 23%. [5] Tamoxifen has not only antiestrogenic but also estrogenic properties depending on the species, tissue, and gene. Tamoxifen displays favorable effects on bone and serum lipid concentrations and stimulation endometrium. [1]

Protocol

Kinase Assay:

[3]

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Competitive binding assays:

Cells are harvested from 150-sq cm T-flasks, and cytosol is prepared at a protein concentration of approximately 2 mg/mL in phosphate buffer. Aliquots of this 180,000 ×g supernatant are then incubated with various concentrations of Tamoxifen and 2.5 nM [3H]estradiol for 16 hr at 0-4 ℃. The free steroids are absorbed by dextran-charcoal [l0 μL of 0.5% Dextran C-5% Norite A in TE buffer] for 1 hr at 0 ℃, and aliquots are counted after centrifugation at 800 ×g, 30 min. The relative binding ability of each competitor is taken as the ratio of the concentration of radioinert estradiol/competitor required to inhibit one-half of the specific [3H]estradiol binding, with the affinity of estradiol set at 100%.
Cell Research:

[3]

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  • Cell lines: Human breast cancer cells MCF-7
  • Concentrations: ~1 μM
  • Incubation Time: 10 days
  • Method:

    MCF-7 cells are seeded into T-25 flasks (1.5×105 cells/flask) and grown for 2 days in the MEM supplemented with 10 mM HEPES buffer, gentamicin (50 μg/mL), penicillin (100 units/mL), streptomycin (0.1 mg/mL), bovine insulin (6 ng/mL), hydrocortisone (3.75 ng/mL), and 5% calf serum that has been treated with dextran-coated charcoal for 45 min at 55 ℃ to remove endogenous hormones. The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen. At the end of incubation, cell numbers are counted.


    (Only for Reference)
Animal Research:

[5]

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  • Animal Models: Human breast carcinoma xenografts MCF-7
  • Dosages: 2 cm Tamoxifen capsules
  • Administration: Subcutaneous implantation
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (177.41 mM)
Water Insoluble
Ethanol ''''100 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.64
Formula

C26H29NO.C6H8O7

CAS No. 54965-24-1
Storage powder
in solvent
Synonyms N/A
Smiles CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3.C(C(=O)O)C(CC(=O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester May 1 2021 Phase 1
NCT04433494 Not yet recruiting Drug: TY-302: capsule 25mg/50mg ; Tamoxifen: tablet10mg Breast Cancer|Solid Tumor TYK Medicines Inc October 2020 Phase 1
NCT04174352 Recruiting Drug: Tamoxifen|Diagnostic Test: FES PET/CT ERα+ Breast Cancer|ESR1 Gene Mutation University of Wisconsin Madison October 20 2020 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?

  • Answer:

    The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID