Tamoxifen (ICI 46474) Citrate

Catalog No.S1972

For research use only.

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Selleck's Tamoxifen (ICI 46474) Citrate has been cited by 14 publications

Purity & Quality Control

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Biological Activity

Description Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.
Targets
ER [1]
(Cell-free assay)
In vitro

Tamoxifen displays antitumor effect due to its antiestrogenic activity (ER). Values for the apparent affinity of Tamoxifen for the ER range between 30 and 0.01% of that obtained for estradiol, dependent on different ER source (species), protein concentration and condition used for assay. Binding of Tamoxifen to ER further leads to inhibition expression of estrogen-regulated genes, including growth factors and angiogenic factors secreted by the tumor that may stimulate growth by autocrine or paracrine mechanisms. Tamoxifen also directly induces programmed cell death. [1] Tamoxifen produces an inhibitory effect on MCF-7 cell [3H]thymidine incorporation and DNA polymerase activity as well as causing a reduction in DNA content of cultures and cell numbers. This inhibitory effect of Tamoxifen on MCF-7 cell growth can be readily reversed by addition of estradiol to the culture medium. 2 and 6 μM Tamoxifen reduces the proportion of cells in S phase and increases the number of cells in G1. At 10 μM, Tamoxifen causes cell death within 48 hr. [2] Tamoxifen inhibits MCF-7 growth with IC50 of ~10 nM after 10 days treatment. Tamoxifen inhibits plasminogen activator activity of MCF-7, and suppresses estradiol-stimulation of plasminogen activator activity. Tamoxifen also evokes minimal increases in cellular progesterone receptor levels. [3] Tamoxifen is able to inhibit the growth of prostate cancer cell PC3, PC3-M, and DU145 with IC50 ranged from 5.5-10 μM, which is related to its inhibition of protein kinase C and induction of p21(waf1/cip1). [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells M3nm[WN6fG:2b4jpZ:Kh[XO|YYm= MUWxPEBp NF7z[nVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFE5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVAvOiEQvF2= NHvVc409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzNlAzPCd-MUm5N|IxOjR:L3G+
human MDA-MB-231 cells MkDZR5l1d3SxeHnjxsBie3OjeR?= MYOxPEBp NVzURYdKS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCxPEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGyMlMh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
human Ishikawa cells NEfMO5BEgXSxdH;4bYPDqGG|c3H5 MV:xPEBp NYSxWW1xS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUXOqaXvhe4Eh[2WubIOgZYZ1\XJiMUigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj61JO69VQ>? M{\mS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOyNFI1Lz5zOUmzNlAzPDxxYU6=
human DU145 cells MVnDfZRwfG:6aXRCpIF{e2G7 MVqxPEBp M{\wOmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KGGodHXyJFE5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVUvOjVizszN NHq4XFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzNlAzPCd-MUm5N|IxOjR:L3G+
MCF-7 MWHGeY5kfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKFt|SG30bJlucWSrbnWgbY5kd3Kyb4LheIlwdiCrboTvJG1ETi15IHPlcIx{NCCLQ{WwQVcvOs7:TR?= MlrBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5M{[zNEc,OTV5M{[zNFww[T5?
MCF-7 MnXVSpVv[3Srb36gZZN{[Xl? MoT6SYZn\WO2aY\lJIRwe2ViZn;yJHs{UF1vIHXzeJJi\GmxbDDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiTVPGMVch[2WubIOsJGVEPTB;MD6wNFAyOc7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ7NkixK|4zPzZ7NkixQE9iRg>?
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MCF-7 M2XhUmZ2dmO2aX;uJIF{e2G7 NETK[W0yKHWP MWfJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDNR2YuPyClZXzsd{BqdiCycnXz[Y5k\SCxZjCxNIUuPiCPIHPvcoNmdnS{YYTpc44hd2ZiW{PIYU0h\XO2cnHkbY9tKCiHMjmsJGlEOzB;Nj61{txO NIfoUZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[5OlgyLz5{N{[5OlgyRC:jPh?=
OHS-50 NGfySXZyUFSVIHHzd4F6 MnrndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= MmHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMUW4O{c,QDJyMUW4O|ww[T5?
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MCF-7 MmH2R5l1d3SxeHnjbZR6KGG|c3H5 MmTaNUB2VQ>? Mmm5R5l1d3SxeHnjbZR6KGGpYXnud5QhVUOILUegZ4VtdHNiaX6gdJJme2WwY3Wgc4YhOSC3TTDFNkAp\XO2cnHkbY9tMSxiQ4n0c5RwgGmlaYT5QVE{|ryP NVfnTJoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUC4PVM{Oid-OUC4PVM{OjxxYU6=
MCF-7 NIXuZ5RHfW6ldHnvckBie3OjeR?= NYjuc3pWUW6qaXLpeIlwdiCxZjDld5Rzd2enbj3pcoR2[2WmIIDyc4xq\mW{YYTpc44hcW5iaIXtZY4hVUOILUegZpJm[XO2IHPhcoNmeiClZXzsd{whUUN3ME2wMlQ5Oc7:TR?= NUm0XotwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUG1OFk3Oyd-OUG1OFk3OzxxYU6=
Ishikawa cells Mn3PRY51cWW|dILv[4VvcWNiYYPzZZk> NYnIZ5p5PCCmYYnz MnPRRY51cWW|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBme3S{b3flck1qdmS3Y3XkJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGGldHn2bZR6KGGodHXyJFQh\GG7czDifUBGVEmVQTygTWM2OD1yLkSy{txO MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86Pzh2MU[zK|46Pzh2MU[zQE9iRg>?
NIH-3T3-G185 NVr1eWlnTnWwY4Tpc44h[XO|YYm= NHn3fG1VWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCGYYXuc5J2[mmlaX6g[YZndHW6IHnuJG5KUC1|VEOtS|E5PSClZXzsd{whUUN3ME22MlTPxE1? Mnj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
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NIH-3T3-G185 M4LRSWZ2dmO2aX;uJIF{e2G7 NG\IO|ZVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCUaH;kZY1qdmViMUKzJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUC9N|EvPM7:TR?= NV\sbG9uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG3NVY2OTRpPkGxO|E3PTF2PD;hQi=>
MCF-7-2a MYXBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> MYPBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDNR2YuPy1{YTDj[YxteyCjczDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIILl[JVk\SCnc4TyZYRqd2xiZX\m[YN1KGK7IEWwKUwhUUN3ME2wMlXPxE1? NGC2Wog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkSzNVA3Oyd-MUK0N|ExPjN:L3G+
MCF-7-2a NFzsXINHfW6ldHnvckBie3OjeR?= MnPOTY5pcWKrdHnvckBw\iCnc4TyZYRqd2xiaX7keYNm\CCnc4Tyc4dmdiC{ZXPldJRweiC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCrbjDNR2YuPy1{YTDj[YxteyxiSVO1NF0xNjYQvF2= NFn2ZWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[3NlI1QSd-MUK2O|IzPDl:L3G+
SK-E2 MYXGeY5kfGmxbjDhd5NigQ>? NY\WdFNPXFChVGLBUnNRV1KWRWK6JIlv[3KnYYPlJIlvKGKxZHnwfUBqdnS{YXPlcIx2dGG{IHHjZ5VufWyjdHnvckApSm:maYD5PkAxNjJidV2pJIlvKFONLVWyJINmdGy|IDjlfJBz\XO|aX7nJGJUTVBrLDDJR|UxRTF3LkVOwG0> M4fXelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O|U6Lz5zMkezPVc2QTxxYU6=
SK-E2 NXjYWJBiTnWwY4Tpc44h[XO|YYm= NGrLZlJVWF:WUlHOV3BQWlSHUkqgbY5kemWjc3WgbY4h\GmqeXTyc4ZtfW:{ZYPj[YlvKGmwdILhZ4VtdHWuYYKgZYNkfW23bHH0bY9vKCimaXj5[JJw\my3b4Lld4NmcW58IEGgeW0qKGmwIGPLMWUzKGOnbHzzJEhmgHC{ZYPzbY5oKEKVRWCpMEBKSzVyPUKzMlPPxE1? M3zT[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O|U6Lz5zMkezPVc2QTxxYU6=
DAOY MYTxTHRUKGG|c3H5 NUGyRXBWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NUmzOUc,OTJ6MkW5N|U9N2F-
MCF-7 M2HJN2Z2dmO2aX;uJIF{e2G7 NUTsV2E2UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgUWNHNTdiYoLlZZN1KHS3bX;yJINmdGy|IIXzbY5oKE2FRj23JIF{e2G7LDDJR|UxRTBwNUlOwG0> M1HuXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEK1PVM2Lz5zMkiyOVk{PTxxYU6=
Caco-2 MmLPSpVv[3Srb36gZZN{[Xl? NF;5VFhVWF:WUlHOV3BQWlSHUkqgeJJidnOncHn0bIVtcWGuIITyZY5{eG:{dDDv[kBTcG:mYX3pcoUhOTJ|IDjiZZNidCC2bzDhdIlk[WxrIHnuJGNi[29vMjDj[YxteyxiSVO1NF0zQc7:TR?= Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV|OE[0PFIoRjF3M{i2OFgzRC:jPh?=
HeLa M1vnV2Z2dmO2aX;uJIF{e2G7 NUjWUohTUW6qaXLpeIlwdiCxZjCxO{1j\XSjLXXzeJJi\GmxbDDt[YRq[XSnZDDseYNq\mW{YYPlJJRz[W6|Y4LpdJRqd25iaX6gTIVN[SClZXzsd{BmgHC{ZYPzbY5oKGi3bXHuJIV{fHKxZ3XuJJJm[2WydH;yJIFteGijOzDFVmUh[XO|YYmsJGlEPTB;MD62NlLPxE1? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi1NUc,OTV4NUi4OVE9N2F-
MCF-7 NXPEbZNSSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MmLLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbY4hVUOILUegbJVu[W5iYoLlZZN1KGOjbnPldkBk\WyuczygTWM2OD1{LkROwG0> M3PL[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFc2Lz5zNU[1PFg4PTxxYU6=
MDA-MB 231 MUnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NHHpOG5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBqdiCPRFGtUWIhOjNzIHj1cYFvKGK{ZXHzeEBk[W6lZYKgZ4VtdHNuIFnDOVA:OTBwNt88US=> NYG5UJJLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW2OVg5PzVpPkG1OlU5QDd3PD;hQi=>
MCF7 NHfJNZJHfW6ldHnvckBie3OjeR?= NH\Mblk,OTBidV2= NIH3enJC\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBGOi2rbnT1Z4VlKGy3Y3nm[ZJie2ViZ3Xu[UB1emGwc3HjeIl3[XSrb36gbY4hVUOINzDj[YxteyCjdDC+NVAhfU1? MmfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5M{WxNlAoRjF4N{O1NVIxRC:jPh?=
MCF7 NYTIT3FySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MXyzJI1oN2up MmXjOFghcHK| Mn[zRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNR2Y4KGOnbHzzJIF1KDNibXevb4cw\GG7IHHmeIVzKDR6IHjyd{whUUN3ME21MlA6|ryP NYLXVZFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5O|k{OzdpPkG2PVc6OzN5PD;hQi=>
MCF7 NEC5TlFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M1L5elczKGi{cx?= MmnmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGFkfGm4aYT5QVQvPs7:TR?= MmfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MUi4NlAoRjF5OUG4PFIxRC:jPh?=
MCF7 NVT6NZlVSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M4TYbFI1KGi{cx?= M4PjeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB{NDDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTBwNUlOwG0> MoT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{N{KyOVYoRjF6MkeyNlU3RC:jPh?=
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ER-positive MCF7 MYLBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> NU\OSpNOOjBibl2= NULQ[nNLPDhiaILz M1y2TmFvfGmnc4Tyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIFXSMZBwe2m2aY\lJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kAyP2KndHGg[ZN1emGmaX;sMYlv\HWlZXSgd4VkemW2ZXSgZYxs[WyrbnWgdIhwe3CqYYThd4Uh[WO2aY\peJkh[XRiMkCgcm0h[W[2ZYKgOFghcHK|IHL5JJBpd3OyaHGtcIlocHRicnXwc5J1\XJiY3jlcYltfW2rbnXzZ4Vv[2ViYYPzZZk> MnO2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEC4OVkoRjJzOECwPFU6RC:jPh?=
HEK293 Mm\1RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NILWVWg1QCCqcoO= NI\6[o1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjFT|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGw{txO NH76bXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3NVgyOid-MkG4O|E5OTJ:L3G+
MCF7 M3;OeGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NVXlXnhNPDhiaILz MYfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{LkS4{txO NHHIVoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3NVgyOid-MkG4O|E5OTJ:L3G+
MDA-MB-231 M1v6cmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NF7yPHk1QCCqcoO= NHiwbINCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zOD65{txO NUjoTYJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O|E5OTJpPkKxPFcyQDF{PD;hQi=>
Ishikawa NWLlZ2g3SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYPWVYZIPDhiaILz Mn;uRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BmgHC{ZYPzbY5oKGW|dILv[4VvKHKnY3XweI9zKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlIvPc7:TR?= Mn3LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
A673 NGLYOnVyUFSVIHHzd4F6 MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR5MkC0OUc,OjJ2N{KwOFU9N2F-
HEK293 NETRTW5HfW6ldHnvckBie3OjeR?= MlvQNU42KGi{cx?= NFvDXmpFcXOybHHj[Y1mdnRib3[gX|NJZUyVRDDmdo9uKGi3bXHuJFVJXDZicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyNjViaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbV0yNjB2Md88US=> M1vNflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUO3NVU{Lz5{MkWzO|E2OzxxYU6=
AH109 M4DEbGZ2dmO2aX;uJIF{e2G7 MXeyOEBpenN? NV7wPZh3SW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoJmfGFicnXj[ZB1d3JiTFLEJIV5eHKnc4Pl[EBqdiC7ZXHzeEBCUDFyOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiSVO1NF0yNjZ4zszN MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
AH109 NIXmdZpHfW6ldHnvckBie3OjeR?= NVL3eWR5OjRiaILz NEXve45CdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFicnXj[ZB1d3JiTFLEJIV5eHKnc4Pl[EBqdiC7ZXHzeEBCUDFyOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG|ZTDhd5NigSxiSVO1NF0zNjV2zszN NIHPZms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[0O|IyPyd-MkK2OFczOTd:L3G+
MCF7 MkGzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2O2[FI1KGi{cx?= MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJJZq[WKrbHn0fUBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG5MlU1|ryP NWLoSpNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
DLD1 NXe5ZoJpS3m2b4TvfIlkcXS7IHHzd4F6 NI\KPJQ6PiCqcoO= M3rYV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRNTDFiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME20Mlc5|ryP NGrjWmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
MCF7 MXTDfZRwfG:6aXPpeJkh[XO|YYm= NWfEXnNHQTZiaILz MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;Nz6x{txO MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
NIH/3T3 NF63VVZEgXSxdH;4bYNqfHliYYPzZZk> MnznPVYhcHK| MoPsR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgUmlJNzOWMzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOjcQvF2= NIe1OpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
518A2 M2XiRmN6fG:2b4jpZ4l1gSCjc4PhfS=> NIH6fZI6PiCqcoO= NWrObWp5S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPTF6QUKgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjZ{zszN MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
A2780 NFHj[3VEgXSxdH;4bYNqfHliYYPzZZk> NF7DeIc6PiCqcoO= MkLYR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME23Mlc4|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
Lipo NGTsdYxEgXSxdH;4bYNqfHliYYPzZZk> MUS5OkBpenN? MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD62OO69VQ>? NIXZdoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
A253 NYDyOYxvS3m2b4TvfIlkcXS7IHHzd4F6 M3PENFk3KGi{cx?= NYrVdlY{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ3MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVgvQTMQvF2= MmfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
A549 NILnb3VEgXSxdH;4bYNqfHliYYPzZZk> NVrkW2xCQTZiaILz MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OT62Ou69VQ>? MlLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
850C Ml;nR5l1d3SxeHnjbZR6KGG|c3H5 NXryN|FCQTZiaILz MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC4OVBEKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MUGuNFnPxE1? M4HCc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
KIF5 NUfud4dyXG:6aXPpeJkh[XO|YYm= M1nTcVI1KGi{cx?= NVznS|lSXG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLTWY2KGOnbHzzJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IFPlcIxVcXSncjDCcJVmKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJ|Lke1{txO NWDQTmp1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3O|U1PzRpPkKyO|c2PDd2PD;hQi=>
MCF7 MmGyR5l1d3SxeHnjbZR6KGG|c3H5 MV63NkBpenN? NWG4[GJbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA4OiCqcoOgZpkhX1OWMTDhd5NigSCrbjDwdoV{\W6lZTDv[kAzOCViRlLTMEBKSzVyPUiuOu69VQ>? NHXlPZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
HL60 MUTDfZRwfG:6aXPpeJkh[XO|YYm= M3f0WFczKGi{cx?= M3zwXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHdUXDFiYYPzZZkhcW5icILld4Vv[2Vib3[gNlAmKE[EUzygTWM2OD1zND6z{txO NHnpOGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
C41 DE3 M{X3cmZ2dmO2aX;uJIF{e2G7 NXvoUHJ[PjBidV2= NEHzSmEyOCCvaX7z M2rWe2lv[WO2aY\heIlwdiCxZjDDXXAzSjZiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDDOFEhTEV|IHPlcIx{KGG|c3Xzd4VlKGG|IH3v[IlncWWmIHHwc{1RPDVyIELCOkBi\GS3Y4Sg[o9zdWG2aX;uJIF1KDZyIIXNJIFnfGW{IEGwJI1qdnNiYomgSXNKNUyFLV3TJIFv[Wy7c3nzJIlvKHC{ZYPlcoNmKG:oIF7BSHBJ NI\FS489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm0NlMyPyd-MkK5OFI{OTd:L3G+
C41 DE3 NGLSPIVHfW6ldHnvckBie3OjeR?= NVPoV45GPjBidV2= NIXlWG1KdmGldHn2ZZRqd25ib3[gR3lROkJ4IDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgR|QyKESHMzDj[YxteyCjc4Pld5Nm\CCjczDtc4Rq\mmnZDDhdI8uWDR3MDCyRlYh[WSmdXP0JIZwem2jdHnvckBifCB4MDD1UUBjgSCHU1mtUGMuVVNiYX7hcJl{cXNiaX6gdJJme2WwY3Wgc4YhVkGGUFigZY5lKEeVSFXF Mo\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEKzNVcoRjJ{OUSyN|E4RC:jPh?=
C41 DE3 MVXGeY5kfGmxbjDhd5NigQ>? MknCNVAxKHWP MVSzNEBucW6| NGfyTphKdmGldHn2ZZRqd25ib3[gR3lROkJ4IDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgR|QyKESHMzDj[YxteyCjc4Pld5Nm\CCjczD0dplxfGmlIIDldJRq\GViMUi4Smh[WUSTRUG5OEB4cXSqIIPpeIUhd2ZiYXTkeYN1KG[xcn3heIlwdiCub3PhcIl7\WRidH:gS4xvOTl|IHH0JFExOCC3TTDh[pRmeiB|MDDtbY5{KGK7IFzDMW1UN02VIHHuZYx6e2m|IHnuJJBz\XOnbnPlJI9nKE6DRGDI MoPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NEKzNVcoRjJ{OUSyN|E4RC:jPh?=
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MDA-MB-231 MWjDfZRwfG:6aXPpeJkh[XO|YYm= MkPGOFghcHK| Mn7DR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{LkSx{txO M1nHO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEKyNlgyLz5{M{CyNlI5OTxxYU6=
MCF7 M1fTcGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mn7COFghcHK| NFTPZYxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR|UxRTFwOENOwG0> MkjGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzMkOwNVcoRjJ|MUKzNFE4RC:jPh?=
DU145 MX7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mk\COFghcHK| NFznWnRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NT61{txO NVj6S5U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFcxPTdpPkKzNlg4ODV5PD;hQi=>
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Ishikawa NYrkTlZuSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUHRdWJLPDhiaILz NY\o[no4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9Ou69VQ>? NH\JcWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G3Olk1PCd-MkexO|Y6PDR:L3G+
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Ishikawa cells NFWy[ZZHfW6ldHnvckBie3OjeR?= M2rRTVczKGi{cx?= MWfBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGW|dILv[4VvKHKnY3XweI9zKGGucHjhM4JmfGFiaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGG|c3H5MEBKSzVyPUCuNljPxE1? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR{NkmzNUc,Ojh2Mk[5N|E9N2F-
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HeLa NELFfIJHfW6ldHnvckBie3OjeR?= MWOyOEBpenN? MWLBcpRi\2:waYP0JIFkfGm4aYT5JIF1KG[3bHygcIVv\3SqIFXSZoV1[SBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDoeY1idiCKZVzhJINmdGy|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEWURT3kdol3\W5ibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUGuOVPPxE1? M3jaO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEiyOVAzLz5{OEi4NlUxOjxxYU6=
MCF7 NVniSolsS3m2b4TvfIlkcXS7IHHzd4F6 NWHjXGZ{PzJiaILz NHnqW2VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBz\XOjeoXybY4h[mG|ZXSg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPUeuPVTPxE1? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl{N{e5OUc,Ojh7Mke3PVU9N2F-
insect cells NVHDS3d1TnWwY4Tpc44h[XO|YYm= NYruSIwyOiCqcoO= NUfnV3Z6TGm|cHzhZ4Vu\W62IH;mJIZtfW:{ZYPj[Y51KGW|dILv[4VvKGyrZ3Hu[EBnem:vIILlZ49u[mmwYX70JIh2dWGwIFXSZYxxcGFiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyCrbnP1ZoF1\WRiZn;yJFIhcHK|IHL5JJBwdGG{aYrheIlwdixiSVO1NF0zNjZ5zszN M1GzUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUSyNVE{Lz5{OEm0NlEyOzxxYU6=
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Ishikawa cells MXXDfZRwfG:6aXPpeJkh[XO|YYm= NXKwXIFHPDhiaILz MmXQR5l1d3SxeHnjbZR6KGmwIHj1cYFvKEm|aHnrZZdiKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCpcn;3eIghcW6ldXLheIVlKG[xcjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKwMlY2|ryP NV72fGh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
MCF7 NHHOTo1HfW6ldHnvckBie3OjeR?= Ml7yNVAhdk1? MVeyOEBpenN? M3PDfWlvcGmkaYTpc44hd2ZiRWLhcJBp[SCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiBzMDDuUUBme3S{b3flck1qdmS3Y3XkJJBzd2enc4Tldo9v\SC{ZXPldJRweiCneIDy[ZN{cW:wIHH0JFEhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? NYT6[nFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF|PD;hQi=>
MCF7 MVzDfZRwfG:6aXPpeJkh[XO|YYm= NFO3XpIzPCCqcoO= NUL5UZVpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSC3cDD0c{AyODBidV2gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?= M{PqPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MES1NVM2Lz5{OUC0OVE{PTxxYU6=
MCF7 MkfQSpVv[3Srb36gZZN{[Xl? M2XobVEhfU1? NU[3Xm5TPCCqcoO= NEfCOVJKdmirYnn0bY9vKG:oIFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kAyP2KndHGt[ZN1emGmaX;sMY1m\GmjdHXkJJBUOiCvUl7BJIV5eHKnc4Ppc44h[XRiMTD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK|IH\vcIxwf2WmIHL5JFE4[mW2YT3ld5Rz[WSrb3ygZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMU[gbJJ{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KgZY5idHm|aYO= NF;3Xno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC0OVE{PSd-MkmwOFUyOzV:L3G+
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MCF7 NXf2RXZYSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Ml3aOFghcHK| NXrFem15SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;OD6xNu69VQ>? NGLoZ289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
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MCF7 NV;aOmg1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUD3blVpPzJiaILz NHr5VG5Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OU4{|ryP M1fkXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkKwO|g6Lz5{OUKyNFc5QTxxYU6=
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Ishikawa cells NULoSnVwSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MkLzQlQxKHWP MlfiOFghcHK| NITXd3ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGG2IE60NEB2VSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6 MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0OEc,Ojl2OEK5OFQ9N2F-
MCF7 M{K4d2Z2dmO2aX;uJIF{e2G7 MVGxJJVO MV\BcpRi\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h\XO2cnHkbY9tNWmwZIXj[YQhWGeUIH3SUmEh\XiycnXzd4lwdiCjdDCxJJVOKGK7IGPZRnIhT3KnZX6g[JlmKGKjc3XkJHJVNVCFUjDhcoFtgXOrcx?= M3L3O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEiyPVQ1Lz5{OUS4Nlk1PDxxYU6=
MCF7 NHzBVIZHfW6ldHnvckBie3OjeR?= MkX2NlQhcHK| MnW1TY5lfWO2aX;uJI9nKHOnbHXjeIl3\SCnc4Tyc4dmdiC{ZXPldJRweiCjbIDoZUBl\We{YXTheIlwdiCrbjDoeY1idiCPQ1[3JINmdGy|IHjhdoJwemmwZzDUT{1GWkVvTIXjJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDld5Rz[WSrb3ytbY5lfWOnZDD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1zLk[wOe69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV4MkezO{c,Ojl3NkK3N|c9N2F-
MCF7 MnLESpVv[3Srb36gZZN{[Xl? MYe2NEBu\y:tZx?= NITK[2Q1QCCmYYnz MYLEc5dvemWpdXzheIlwdiCxZjDwdo9o\XO2ZYLvcoUhemWlZYD0c5IhdVKQQTDs[ZZmdCCrbjDOU2QhW0OLRDDnZY1u[SCvb4Xz[UB5\W6xZ4Lh[pRm\CC5aYToJIh2dWGwIF3DSlch[2WubIOgZZQhPjBibXevb4ctKHCxIEWg[IF6eyCyZYKge4VmcyCob4KgOFgh\GG7czDwc5N1KHS3bX;yJIlueGyjboTheIlwdiCvZXHzeZJm\CB5IHjyd{Bxd3O2IHzhd5Qh\G:|ZTDifUBTXC2SQ2KgcYV1cG:m NU[1OnZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OlI4OzdpPkK5OVYzPzN5PD;hQi=>
insect cells MoXNSpVv[3Srb36gZZN{[Xl? MkjaNkBpenN? NHfibHVFcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIH\1cIwhdGWwZ4ToJIh2dWGwIFXSMYFteGijIHX4dJJme3OnZDDpckBqdnOnY4SgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIIDvcIFzcXqjdHnvckBie3OjeTygTWM2OD1zLkSy{txO NVrScFF5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFczOjFpPkK5OVg4OjJzPD;hQi=>
MCF7 MkHlRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MlruOFghcHK| M3;TN2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF3LkS2{txO MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6N{KyNUc,Ojl3OEeyNlE9N2F-
Ishikawa cells MkjERY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXjtPHR1PDhiaILz MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEm|aHnrZZdiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mk[uOVLPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6N{KyNUc,Ojl3OEeyNlE9N2F-
MRC5 SV2 Ml;RR5l1d3SxeHnjbZR6KGG|c3H5 NFLsfGI4OiCqcoO= MmrzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXJEPSCVVkKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWtZoF{\WRiZnz1c5JqdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUGwMlLPxE1? NXvKSWk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFQ2PDFpPkK5OlA1PTRzPD;hQi=>
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Lucena 1 MnzuSpVv[3Srb36gZZN{[Xl? M2f0SVEzNjVidV2= M3\i[lEhcHJ? Ml:0TY5pcWKrdHnvckBw\iCSLXfwMY1m\GmjdHXkJHJpdy1zMkOg[YZndHW6IHnuJIh2dWGwIFz1Z4Vv[SBzIHPlcIx{KGG2IEGyMlUhfU1icILlbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KFKqbz2xNlMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNUBpeiCkeTDmcI94KGO7dH;t[ZRzgQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyOEc,OzB4MUOzNlQ9N2F-
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MDA-MB-231 MmXLR5l1d3SxeHnjbZR6KGG|c3H5 MlXLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczygS2k2OD1yLkK0{txO NFjtOXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUKwNFI{Pid-M{GyNFAzOzZ:L3G+
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MCF7 NV;FXZUxSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUGwMlg4|ryP MoXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3NE[xPVcoRjNzNUS2NVk4RC:jPh?=
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PANC1 M{\ScWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWTQPZZ1T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hWEGQQ{GgZ4VtdHNuIFfJOVA:OC5zMt88US=> MljYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MEW4OlUoRjNzNkC1PFY2RC:jPh?=
MCF7 NHXyeVNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NFXWZYJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZpkh\my3b4Lld4NmdmOnIHLhd4VlKH[rYXLs[UBk\WyuIHPveY51cW6pIH3leIhw\CxiSVO1NF0zPc7:TR?= NVvpXnltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G2NVA{PzhpPkOxOlExOzd6PD;hQi=>
M-HeLa MmrQRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGP3fHFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF2tTIVN[SClZXzsd{BjgSCobIXvdoV{[2WwY3WgZoF{\WRidnnhZoxmKGOnbHygZ492dnSrbnegcYV1cG:mLDDJR|UxRTJ6zszN MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZzMEO3PEc,OzF4MUCzO|g9N2F-
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MCF7 MVrDfZRwfG:6aXPpeJkh[XO|YYm= M1j4Z|Q5KGi{cx?= MonIR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVIzNjcQvF2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZ{MEKyOUc,OzF4MkCyNlU9N2F-
RAW264.7 NUK2fJMyS3m2b4TvfIlkcXS7IHHzd4F6 NG\QeVQzPCCqcoO= NWXETJh3S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBv\XW2cnHsJJJm\CCmeXWgZoF{\WRiYYPzZZktKEOFNUC9NVEvOc7:TR?= M{jxZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzN{CzPFE5Lz5|MUewN|gyQDxxYU6=
Assay
Methods Test Index PMID
Western blot p-AKT / AKT ; ER-α66 / ER-α36 / HER2 / EGFR ; p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; CD36 / p-ERK / ERK 30759864 25203051 27020861 30573731
Immunofluorescence CD36 30573731
In vivo

Tamoxifen administration to rapidly growing, estradiol-stimulated MCF-7 xenografts results in a dose-dependent retardation or cessation of tumor growth by significantly decreasing tumor cell proliferation in tumor. Tamoxifen treatment results in a slowing of tumor growth (tumor doubling time, 12 days), a significant increase in tumor potential doubling time (Tpot) (6.6 days), and a decrease in labeling index (%LI) (to 8%) by 23 days posttreatment, compared with untreated mice which shows a volume doubling time of 5 days, a Tpot of 2.3 days, and a %LI of 23%. [5] Tamoxifen has not only antiestrogenic but also estrogenic properties depending on the species, tissue, and gene. Tamoxifen displays favorable effects on bone and serum lipid concentrations and stimulation endometrium. [1]

Protocol (from reference)

Kinase Assay:

[3]

  • Competitive binding assays:

    Cells are harvested from 150-sq cm T-flasks, and cytosol is prepared at a protein concentration of approximately 2 mg/mL in phosphate buffer. Aliquots of this 180,000 ×g supernatant are then incubated with various concentrations of Tamoxifen and 2.5 nM [3H]estradiol for 16 hr at 0-4 ℃. The free steroids are absorbed by dextran-charcoal [l0 μL of 0.5% Dextran C-5% Norite A in TE buffer] for 1 hr at 0 ℃, and aliquots are counted after centrifugation at 800 ×g, 30 min. The relative binding ability of each competitor is taken as the ratio of the concentration of radioinert estradiol/competitor required to inhibit one-half of the specific [3H]estradiol binding, with the affinity of estradiol set at 100%.

Cell Research:

[3]

  • Cell lines: Human breast cancer cells MCF-7
  • Concentrations: ~1 μM
  • Incubation Time: 10 days
  • Method:

    MCF-7 cells are seeded into T-25 flasks (1.5×105 cells/flask) and grown for 2 days in the MEM supplemented with 10 mM HEPES buffer, gentamicin (50 μg/mL), penicillin (100 units/mL), streptomycin (0.1 mg/mL), bovine insulin (6 ng/mL), hydrocortisone (3.75 ng/mL), and 5% calf serum that has been treated with dextran-coated charcoal for 45 min at 55 ℃ to remove endogenous hormones. The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen. At the end of incubation, cell numbers are counted.

  • (Only for Reference)
Animal Research:

[5]

  • Animal Models: Human breast carcinoma xenografts MCF-7
  • Dosages: 2 cm Tamoxifen capsules
  • Administration: Subcutaneous implantation
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(177.41 mM)
Water Insoluble
Ethanol ''''100 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.

30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 563.64
Formula

C26H29NO.C6H8O7

CAS No. 54965-24-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3.C(C(=O)O)C(CC(=O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05156892 Not yet recruiting Drug: SUBA-itraconazole|Drug: Tamoxifen Ovarian Cancer Anthony Joshua FRACP|Royal Prince Alfred Hospital Sydney Australia|Concord Hospital|Prince of Wales Hospital Sydney|St Vincent''s Hospital Sydney February 1 2022 Phase 1
NCT05133674 Not yet recruiting Drug: Tamoxifen 20 mg Breast Cancer|Breast Carcinoma|Breast Tumors|Cancer of Breast|Malignant Neoplasm of Breast Karolinska University Hospital January 10 2022 Phase 2
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester December 1 2021 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?

Answer:
The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

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