Tamoxifen (ICI 46474) Citrate

Name: Tamoxifen (ICI 46474) Citrate
Brand: Selleck Chemicals
SKU: S1972
Rating: 5 (14 reviews)

For research use only.

Catalog No.S1972

14 publications

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Tamoxifen Citrate (ICI 46474) is a selective estrogen receptor modulator (SERM). Tamoxifen Citrate is also a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen induces apoptosis and autophagy.

Selleck's Tamoxifen (ICI 46474) Citrate has been cited by 14 publications

Clin Cancer Res, 2021, 10.1158/1078-0432.CCR-20-3905

PubMed: 34011560 ( click the link to review the publication )

Cancers (Basel), 2021, 13(9)2116

PubMed: 33925607 ( click the link to review the publication )

Cell Death Dis, 2021, 12(6):619

PubMed: 34131114 ( click the link to review the publication )

Front Oncol, 2021, 11:681530

PubMed: 34277427 ( click the link to review the publication )

ACS Biomater Sci Eng, 2021, 10.1021/acsbiomaterials.0c01803

PubMed: 33688723 ( click the link to review the publication )

BMC Dev Biol, 2021, 21(1):4

PubMed: 33517884 ( click the link to review the publication )

Biomedicines, 2021, 9(6)681

PubMed: 34208714 ( click the link to review the publication )

Acta Biomater, 2020, S1742-7061(20)30695-4

PubMed: 33271355 ( click the link to review the publication )

Sci Signal, 2020, 13(663)eaax7119

PubMed: 33443209 ( click the link to review the publication )

Molecules, 2020, 25(15)E3355

PubMed: 32722075 ( click the link to review the publication )

Molecules, 2020, 25(20)E4606

PubMed: 33050377 ( click the link to review the publication )

Cell Chem Biol, 2019, 26(3):420-432

PubMed: 30686757 ( click the link to review the publication )

Med Sci Monit, 2019, 25:8905-8912

PubMed: 31760402 ( click the link to review the publication )

University of North Texas, 2014, Timothy E. Richardson

PubMed: ( click the link to review the publication )

1 Customer Review

Effects of DPN, LY500307, Raloxifene and Tamoxifen on cell viability in BSO-treated FRDA fibroblasts. BSO concentration was 1 mM and all steroid concentrations were 100 nM. Depicted are mean ± SD for n= 8 per group. * indicated p<0.05 versus BSO alone-treated cells.

University of North Texas Health Science Center, 2014.. Tamoxifen (ICI 46474) Citrate purchased from Selleck.

Purity & Quality Control

Choose Selective Estrogen/progestogen Receptor Inhibitors

Biological Activity

Description

Targets

ER ^[1]
(Cell-free assay)

In vitro

Tamoxifen displays antitumor effect due to its antiestrogenic activity (ER). Values for the apparent affinity of Tamoxifen for the ER range between 30 and 0.01% of that obtained for estradiol, dependent on different ER source (species), protein concentration and condition used for assay. Binding of Tamoxifen to ER further leads to inhibition expression of estrogen-regulated genes, including growth factors and angiogenic factors secreted by the tumor that may stimulate growth by autocrine or paracrine mechanisms. Tamoxifen also directly induces programmed cell death. ^[1] Tamoxifen produces an inhibitory effect on MCF-7 cell [³H]thymidine incorporation and DNA polymerase activity as well as causing a reduction in DNA content of cultures and cell numbers. This inhibitory effect of Tamoxifen on MCF-7 cell growth can be readily reversed by addition of estradiol to the culture medium. 2 and 6 μM Tamoxifen reduces the proportion of cells in S phase and increases the number of cells in G1. At 10 μM, Tamoxifen causes cell death within 48 hr. ^[2] Tamoxifen inhibits MCF-7 growth with IC50 of ~10 nM after 10 days treatment. Tamoxifen inhibits plasminogen activator activity of MCF-7, and suppresses estradiol-stimulation of plasminogen activator activity. Tamoxifen also evokes minimal increases in cellular progesterone receptor levels. ^[3] Tamoxifen is able to inhibit the growth of prostate cancer cell PC3, PC3-M, and DU145 with IC50 ranged from 5.5-10 μM, which is related to its inhibition of protein kinase C and induction of p21(waf1/cip1). ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
human MCF7 cells	MXjDfZRwfG:6aXRCpIF{e2G7		NH7lfmgyQCCq	M4rvbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgNVghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE4zKM7:TR?=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl\|MkCyOEc,OTl7M{KwNlQ9N2F-
human MDA-MB-231 cells	NY\QfZRIS3m2b4TvfIlkyqCjc4PhfS=>		NWTTd\|l3OThiaB?=	M{\MTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZYZ1\XJiMUigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj6zJO69VQ>?	NIDTOVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzNlAzPCd-MUm5N\|IxOjR:L3G+
human Ishikawa cells	Mny4R5l1d3SxeHnjxsBie3OjeR?=		NUPsWWNROThiaB?=	MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjZoTldkAyQCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEzNjVizszN	M3HoNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUOyNFI1Lz5zOUmzNlAzPDxxYU6=
human DU145 cells	MUHDfZRwfG:6aXRCpIF{e2G7		NYDCVWRUOThiaB?=	NW[zVlBJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPS5{NTFOwG0>	NYjtfnYzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5N\|IxOjRpPkG5PVMzODJ2PD;hQi=>
MCF-7	M3PxSmZ2dmO2aX;uJIF{e2G7			NWnKT5E3UW6qaXLpeIlwdiCxZjDbN2hefGi7bXnkbY5mKGmwY3;ydI9z[XSrb36gbY51dyCPQ1[tO{Bk\WyuczygTWM2OD15LkNOwG0>	MoTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5M{[zNEc,OTV5M{[zNFww[T5?
MCF-7	NXjhSmNsTnWwY4Tpc44h[XO\|YYm=			MlH5SYZn\WO2aY\lJIRwe2ViZn;yJHs{UF1vIHXzeJJi\GmxbDDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiTVPGMVch[2WubIOsJGVEPTB;MD6wNFAyOc7:TR?=	MlnWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OU[4NUc,Ojd4OU[4NVww[T5?
BT-20	M{TPS2Z2dmO2aX;uJIF{e2G7	M{DQOFExKHWP		NX;IfIlwUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KHC{b3zp[oVz[XSrb36gc4YhSlRvMkCgZ4VtdHNiaX6gdJJme2WwY3Wgc4YhXFCHIHH0JFEx\S13IF2gZ49v[2WwdILheIlwdixiSVOzNF02NjgQvF2=	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ7NkixK\|4zPzZ7NkixQE9iRg>?
MCF-7	M2fTe2Z2dmO2aX;uJIF{e2G7	NUfJdno3OSC3TR?=		MnrMTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JJBzd2yrZnXyZZRqd25ib3[gUWNHNTdiY3XscJMhcW5icILld4Vv[2Vib3[gNVBmNTZiTTDjc45k\W62cnH0bY9vKG:oIGuzTH0uKGW\|dILh[IlwdCBqRUKpMEBKSzNyPU[uOe69VQ>?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ7NkixK\|4zPzZ7NkixQE9iRg>?
OHS-50	NXniPXVMeUiWUzDhd5NigQ>?			MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy\|	MlfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQDJyMUW4O{c,QDJyMUW4O\|ww[T5?
MCF-7	MlnwR5l1d3O2YYTpZ4l1gSCjc4PhfS=>	MmDWNUB2VQ>?		MnHGR5l1d3O2YYTpZ4l1gSCjZ3HpcpN1KE2FRj23JINmdGy\|IHnuJJRp\SCycnXz[Y5k\SCxZjCxJJVOd2yjcjDFNkAp\XO2cnHkbY9tMSxiSVO1NF05\|ryP	MmfvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTB6OUOzNkc,QTB6OUOzNlww[T5?
MCF-7	M3jmd2N6fG:2b4jpZ4l1gSCjc4PhfS=>	M{nue\|EhfU1?		NX3nc\|hQS3m2b4TvfIlkcXS7IHHnZYlve3RiTVPGMVch[2WubIOgbY4hfGinIHHid4Vv[2Vib3[gNUB2VSCHMjCo[ZN1emGmaX;sLUwhS3m2b4TvfIlkcXS7PUmuPO69VQ>?	Mom4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTB6OUOzNkc,QTB6OUOzNlww[T5?
MCF-7	NWS4TVJCS3m2b4TvfIlkcXS7IHHzd4F6	NHqwRpcyKHWP		NWW1WGNkS3m2b4TvfIlkcXS7IHHnZYlve3RiTVPGMVch[2WubIOgbY4heHKnc3XuZ4Uhd2ZiMTD1UUBGOiBqZYP0doFlcW:uKTygR5l1d3SxeHnjbZR6RTF\|zszN	NF35NmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97MEi5N\|MzLz57MEi5N\|MzRC:jPh?=
MCF-7	NVnPRZZbTnWwY4Tpc44h[XO\|YYm=			MlTkTY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2rbnT1Z4VlKHC{b3zp[oVz[XSrb36gbY4hcHWvYX6gUWNHNTdiYoLlZZN1KGOjbnPldkBk\WyuczygTWM2OD1yLkS4Ne69VQ>?	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86OTV2OU[zK\|46OTV2OU[zQE9iRg>?
Ishikawa cells	NU[4[WNmSW62aXXzeJJw\2WwaXOgZZN{[Xl?		MYO0JIRigXN?	NVHIOll[SW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gTZNpcWujd3GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2rbnT1Z4VlKGGua3HsbY5mKHCqb4PwbIF1[XOnIHHjeIl3cXS7IHHmeIVzKDRiZHH5d{BjgSCHTFnTRUwhUUN3ME2wMlQz\|ryP	M4O1fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl5OESxOlMoRjl5OESxOlM9N2F-
NIH-3T3-G185	M{Ttd2Z2dmO2aX;uJIF{e2G7			MX7UVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBF[XWwb4L1ZolkcW5iZX\mcJV5KGmwIF7JTE0{XDNvR{G4OUBk\WyuczygTWM2OD14LkVOwG0>	M2ryS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzN{G2OVE1Lz5zMUexOlUyPDxxYU6=
NIH-3T3-G185	M{jTOmZ2dmO2aX;uJIF{e2G7			NUnkc2c5XFChVGLBUnNRV1KWRWK6JIlvcGmkaYTpc44hd2ZiTFTTMVc2OSCnZn\seZghcW5iTlnIMVNVOy2JMUi1JINmdGy\|LDDJR\|UxRTF{LkJOwG0>	MlPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF5MU[1NVQoRjFzN{G2OVE1RC:jPh?=
NIH-3T3-G185	Ml71SpVv[3Srb36gZZN{[Xl?			NE\6ZZZVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCUaH;kZY1qdmViMUKzJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUC9N\|EvPM7:TR?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
MCF-7-2a	MXjBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>			MnHvRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gUWNHNTdvMnGgZ4VtdHNiYYOgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDy[YR2[2ViZYP0doFlcW:uIHXm[oVkfCCkeTC1NEUtKEmFNUC9NE42\|ryP	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR\|MUC2N{c,OTJ2M{GwOlM9N2F-
MCF-7-2a	MXvGeY5kfGmxbjDhd5NigQ>?			NUfm[3d5UW6qaXLpeIlwdiCxZjDld5Rz[WSrb3ygbY5lfWOnZDDld5Rzd2enbjDy[YNmeHSxcjD0doFve2O{aYD0bY9v[WxiYXP0bZZifGmxbjDpckBOS0ZvNz2yZUBk\WyuczygTWM2OD1yLkZOwG0>	MkjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4N{KyOFkoRjF{NkeyNlQ6RC:jPh?=
SK-E2	NF7YeVJHfW6ldHnvckBie3OjeR?=			M{nXOnRRZ1SUQV7TVG9TXEWUOjDpcoNz\WG\|ZTDpckBjd2SrcImgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gLGJw\GmyeUqgNE4zKHWPKTDpckBUUy2HMjDj[YxteyBqZYjwdoV{e2mwZzDCV2VRMSxiSVO1NF0yPS52zszN	M4XrZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O\|U6Lz5zMkezPVc2QTxxYU6=
SK-E2	NFfzUFVHfW6ldHnvckBie3OjeR?=			Mlj1WHBgXFKDTmPQU3JVTVJ8IHnuZ5Jm[XOnIHnuJIRqcHmmcn;mcJVwemW\|Y3XpckBqdnS{YXPlcIx2dGG{IHHjZ5VufWyjdHnvckAp\GmqeXTyc4ZtfW:{ZYPj[YlvQiBzIIXNLUBqdiCVSz3FNkBk\WyuczCo[ZhxemW\|c3nu[{BDW0WSKTygTWM2OD1{Mz6z{txO	M3G0[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{O5O\|U6Lz5zMkezPVc2QTxxYU6=
DAOY	MoXXdWhVWyCjc4PhfS=>			NHi1dZByUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjh{NUmzOUc,OTJ6MkW5N\|U9N2F-
MCF-7	M2K5dmZ2dmO2aX;uJIF{e2G7			NVvWRottUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgUWNHNTdiYoLlZZN1KHS3bX;yJINmdGy\|IIXzbY5oKE2FRj23JIF{e2G7LDDJR\|UxRTBwNUlOwG0>	M2\ZSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEK1PVM2Lz5zMkiyOVk{PTxxYU6=
Caco-2	NGfsdmFHfW6ldHnvckBie3OjeR?=			NUDpS3VxXFChVGLBUnNRV1KWRWK6JJRz[W6\|ZYDpeIhmdGmjbDD0doFve3CxcoSgc4YhWmixZHHtbY5mKDF{MzCoZoF{[WxidH:gZZBq[2GuKTDpckBE[WOxLUKgZ4VtdHNuIFnDOVA:OjoQvF2=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTN6NkS4Nkc,OTV\|OE[0PFI9N2F-
HeLa	NHPjfJRHfW6ldHnvckBie3OjeR?=			M2nYNWlvcGmkaYTpc44hd2ZiMUetZoV1[S2nc4TyZYRqd2xibXXkbYF1\WRibIXjbYZmemG\|ZTD0doFve2O{aYD0bY9vKGmwIFjlUIEh[2WubIOg[ZhxemW\|c3nu[{BpfW2jbjDld5Rzd2enbjDy[YNmeHSxcjDhcJBp[TtiRWLFJIF{e2G7LDDJR\|UxRTBwNkKy{txO	NXy0dINFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW2OVg5PTFpPkG1OlU5QDVzPD;hQi=>
MCF-7	NXLLNW44SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=			NHGxcHFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBqdiCPQ1[tO{BpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHzzMEBKSzVyPUKuN:69VQ>?	NGLMXXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[1PFg4PSd-MUW2OVg5PzV:L3G+
MDA-MB 231	MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			M2PRZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIlvKE2GQT3NRkAzOzFiaIXtZY4h[nKnYYP0JINidmOncjDj[YxteyxiSVO1NF0yOC54zszN	NF21WmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[1PFg4PSd-MUW2OVg5PzV:L3G+
MCF7	MXrGeY5kfGmxbjDhd5NigQ>?	M4XPdV4yOCC3TR?=		NVTpPXFvSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhTTJvaX7keYNm\CCudXPp[oVz[XOnIHflcoUhfHKjboPhZ5RqfmG2aX;uJIlvKE2FRkegZ4VtdHNiYYSgQlExKHWP	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd\|NUGyNEc,OTZ5M{WxNlA9N2F-
MCF7	NYDSO4NnSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	M4jvdFMhdWdxa3e=	Mnn5OFghcHK\|	NEO2dnFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JG1ETjdiY3XscJMh[XRiMzDt[{9s\y:mYYmgZYZ1\XJiNEigbJJ{NCCLQ{WwQVUvODoQvF2=	M2DoflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUe5N\|M4Lz5zNkm3PVM{PzxxYU6=
MCF7	M4OxTWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NYHaOmdZPzJiaILz	M{W1W2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG\|c3H5MEBC[3Srdnn0fV01NjcQvF2=	MlzDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MUi4NlAoRjF5OUG4PFIxRC:jPh?=
MCF7	NX;XV\|ZxSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NFPGU5AzPCCqcoO=	MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0xNjV6zszN	NWnmWIU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyO\|IzPTZpPkG4NlczOjV4PD;hQi=>
insect cells	MVjGeY5kfGmxbjDhd5NigQ>?			M1O1bmRqe3CuYXPlcYVvfCCxZjDmcJVwemW\|Y3XpckBt[WKnbHXkJIV{fHKjZHnvcEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFXSZYxxcGFiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBk\WyuczDifUBndHWxcnXzZ4Vv[2VicH;sZZJqgmG2aX;uJIF{e2G7LDDJR\|UxRTBwMEhOwG0>	NFfLXFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEizOVE4Pid-MUi4N\|UyPzZ:L3G+
insect cells	NXvaTIkzTnWwY4Tpc44h[XO\|YYm=			MnzySIl{eGyjY3Xt[Y51KG:oIH\seY9z\XOlZXnuJIxi[mWuZXSg[ZN1emGmaX;sJIZzd21iaIXtZY4hemWlb33ibY5idnRiRWLi[ZRiKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{Bqdm[nY4Tl[EBqdnOnY4SgZ4VtdHNiYomg[ox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigSxiSVO1NF0xNjF5zszN	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh\|NUG3Okc,OTh6M{WxO\|Y9N2F-
Ishikawa	M2TzOWFvfGmnc4Tyc4dmdmmlIHHzd4F6		NUPTPVhUPzJiaILz	MXTBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCLc3jpb4F4[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIV{fHKxZ3XuMYlv\HWlZXSgZYxs[WyrbnWgdIhwe3CqYYThd4Uh[WO2aY\peJkh[W[2ZYKgO\|IhcHK\|LDDJR\|UxRTBwMUhOwG0>	MlT4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh6M{WxO\|YoRjF6OEO1NVc3RC:jPh?=
MCF7	NIHPN3RCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M4LkSVczKGi{cx?=	MmrZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDld5Rzd2enbjDy[YNmeHSxcjDk[ZBmdmSnboSgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTRwMUNOwG0>	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDh\|NUG3Okc,OTh6M{WxO\|Y9N2F-
T47D	NGLiOYlCdnSrZYP0do9o\W6rYzDhd5NigQ>?			NHH5UGpCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDUOFdFKGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5Ih[XO\|ZYPz[YQh[XNiZYP0do9o\W5vZHXw[Y5l\W62IITyZY5{[3KrcITpc44h[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBKSzVyPUCuNe69VQ>?	NVfuTIVxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN\|EzPDhpPkG5NVMyOjR6PD;hQi=>
T47D	M3S1WGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7		M1;O[lczKGi{cx?=	MXTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDld5Rzd2enbj3k[ZBmdmSnboSgWFQ4TCClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:Oc7:TR?=	NEfHOmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGzNVI1QCd-MUmxN\|EzPDh:L3G+
HEK293	MkS2SpVv[3Srb36gZZN{[Xl?		MVezNEBucW6\|	MXPTeIlufWyjdHnvckBw\iCJRmCteIFo\2WmIHj1cYFvKFCOREKg[ZhxemW\|c3XkJIlvKGi3bXHuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDwbI9{eGijdHnkfYxjfXSjbn;sMXtlQV1icILv[JVkfGmxbjDh[pRmeiB\|MDDtbY5{KGK7IH3hd5Mhe3CnY4Tyc41mfHKrYzDhcoFtgXOrcx?=	NYfxcZJiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN\|Y6PzVpPkG5NVM3QTd3PD;hQi=>
MCF7	NES1NHpCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NFT5W3M1QCCqcoO=	M1j1bWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO\|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUFOwG0>	MnTxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MkOzOVYoRjF7NEKzN\|U3RC:jPh?=
MDA-MB-231	NVjHOIxXSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NF[0V4c1QCCqcoO=	M1LGSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iZYP0do9o\W5icnXj[ZB1d3JiZHXmbYNq\W62IF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zOM7:TR?=	NXXyblM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NlM{PTZpPkG5OFI{OzV4PD;hQi=>
MCF7	MVXBcpRq[2GwY3XyJIF{e2G7		Mnz5NVghcHK\|	NIfqSoJCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5Qh\XO2cn;n[Y4ueG:\|aYTpeoUhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjCxPEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUGy{txO	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR2NkmzNEc,OTl2NE[5N\|A9N2F-
MDA-MB-231	MXPBcpRq[2GwY3XyJIF{e2G7		NUXpWWd4OThiaILz	NHnOfZVCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5Qh\XO2cn;n[Y4ueG:\|aYTpeoUhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiBzODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTJ2zszN	M4rEVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NES2PVMxLz5zOUS0Olk{ODxxYU6=
NCI-H460	MV\BcpRq[2GwY3XyJIF{e2G7		NILGdpYzPCCqcoO=	M1m2NWFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkAzPCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVQvPDkQvF2=	NUDWTJFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3N\|MxQDVpPkG5O\|M{ODh3PD;hQi=>
DU145	MXjBcpRq[2GwY3XyJIF{e2G7		MmjQNlQhcHK\|	M{naTWFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjCyOEBpenNiYomgV3JDKGG\|c3H5MEBIUTVyPU[uNFfPxE1?	M1fxdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{OzNFg2Lz5zOUezN\|A5PTxxYU6=
SKOV3	M4ryfGFvfGmlYX7j[ZIh[XO\|YYm=		M2PYZ\|I1KGi{cx?=	MUnBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vPWlMh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGPSRkBie3OjeTygS2k2OD14LkVOwG0>	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd\|M{C4OUc,OTl5M{OwPFU9N2F-
MCF7	MmLHRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MWi0PEBpenN?	NEXQSnZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15Lk[1{txO	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTd2ME[2O{c,OTl5NEC2Olc9N2F-
MDA-MB-231	NFPFRZBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MXS0PEBpenN?	MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME25Mlg3\|ryP	NGDUWos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUe0NFY3Pyd-MUm3OFA3Pjd:L3G+
MCF7	MVjDfZRwfG:6aXPpeJkh[XO\|YYm=			M2S5ZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJIFlemmjbYnjbY4uKHKnc3nzeIFvfCCPQ1[3JINmdGy\|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwQVExNjoQvF2=	NHr3c2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUizOlI{OCd-MUm4N\|YzOzB:L3G+
MCF7	M3fnOmN6fG:2b4jpZ4l1gSCjc4PhfS=>			NV\RcnhtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hfGGvb4jp[oVvNXKnc3nzeIFvfCCPQ1[3JINmdGy\|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwQVEyNjIQvF2=	NWrHU\|NzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4N\|YzOzBpPkG5PFM3OjNyPD;hQi=>
MCF7	M{XXU2N6fG:2b4jpZ4l1gSCjc4PhfS=>			M3TvVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[nliV2PUMVEh[XO\|YYmsJGlEPTB;MUGuOO69VQ>?	M3zoTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEO2NlMxLz5zOUizOlI{ODxxYU6=
MDA-MB-231	M1v2SWN6fG:2b4jpZ4l1gSCjc4PhfS=>			MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwQVEzNjUQvF2=	Mnr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6M{[yN\|AoRjF7OEO2NlMxRC:jPh?=
MCF7	NHLPXVhCdnSrZYP0do9o\W6rYzDhd5NigQ>?		MkiyPVYhcHK\|	MYPBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{BqdmirYnn0bY9vKG:oIEGwNEBxVSCHMj3lcohidmOnZDDj[YxtKHC{b3zp[oVz[XSrb36geI8hdGW4ZXyg[ZF2cX[jbHXueEB1dyB3MDDwUUBGOiCjZoTldkA6PiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTBwMd88US=>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl2M{[yNEc,OTl7NEO2NlA9N2F-
T47D	MVzBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		Mnz1PVYhcHK\|	MX3BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCWNEfEJINmdGy\|IHHzd4V{e2WmIHHzJIRzfWdibHX2[Ywh[2G3c3nu[{BqdmirYnn0bY9vKG:oIEGwNEBxVSCHMj3lcohidmOnZDDj[YxtKHC{b3zp[oVz[XSrb36geI8hdGW4ZXyg[ZF2cX[jbHXueEB1dyB3MDDwUUBGOiCjZoTldkA6PiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTBwMd88US=>	NU[4XZBKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OFM3OjBpPkG5PVQ{PjJyPD;hQi=>
MCF7	MkPMRY51cWW\|dILv[4VvcWNiYYPzZZk>		NFzXSpA6PiCqcoO=	NW\sWYRGSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCmcoXnJIxmfmWuIHPheZNqdmdiaX7obYJqfGmxbjDv[kAyODBicF2gSVIu\W6qYX7j[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHzleoVtKGWzdXn2ZYxmdnRidH:gNVAheE1iRUKgZYZ1\XJiOU[gbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlLPxE1?	MoLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7NEO2NlAoRjF7OUSzOlIxRC:jPh?=
MCF7	M2TESmFvfGmnc4Tyc4dmdmmlIHHzd4F6		Mn36PVYhcHK\|	NFLnN5pCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGS{dXegcIV3\WxiY3H1d4lv\yCrbnjpZol1cW:wIH;mJFExOCCyTTDFNk1mdmijbnPl[EBk\WyuIIDyc4xq\mW{YYTpc44hfG9ibHX2[Ywh\XG3aY\hcIVvfCC2bzCxJJBOKEV{IHHmeIVzKDl4IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MD60{txO	NIfEPYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm0N\|YzOCd-MUm5OFM3OjB:L3G+
T47D	NWPWc4JHSW62aXXzeJJw\2WwaXOgZZN{[Xl?		MmrwPVYhcHK\|	MkLpRY51cWW\|dILv[4VvcWNiYXP0bZZqfHliaX6gbJVu[W5iVES3SEBk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKGOjdYPpcochcW6qaXLpeIlwdiCxZjCxNFAheE1iRUKt[Y5p[W6lZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGyndnXsJIVyfWm4YXzlcpQhfG9iMUCgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkhOwG0>	NHXQS289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm0N\|YzOCd-MUm5OFM3OjB:L3G+
T47D	MWLBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		M4WxWVk3KGi{cx?=	M376NGFvfGmnc4Tyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIGS0O2Qh[2WubIOgZZN{\XO\|ZXSgZZMh\HK3ZzDs[ZZmdCClYYXzbY5oKGmwaHnibZRqd25ib3[gNVAxKHCPIFWyMYVvcGGwY3XkJINmdGxicILvcIln\XKjdHnvckB1dyCuZY\lcEBmeXWrdnHs[Y51KHSxIEGgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT17zszN	M4P2TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUSzOlIxLz5zOUm0N\|YzODxxYU6=
MDA-MB-231	MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			MUTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[nliTWTUJIF{e2G7LDDJR\|UxRTBwNkdOwG0>	NXfYdmg1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVE{QDBpPkKwOFUyOzhyPD;hQi=>
MCF7	MUXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			NGq0UmZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVHPxE1?	MmjvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUGzPFAoRjJyNEWxN\|gxRC:jPh?=
HepG2	MoPHR5l1d3SxeHnjbZR6KGG\|c3H5		MVOyOEBpenN?	NUPtNmU{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KEOnbHzUbZRmei2EbIXlR4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvPM7:TR?=	M4T2Z\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUS1N\|M1Lz5{MEW0OVM{PDxxYU6=
HT-29	NW\0WmNlS3m2b4TvfIlkcXS7IHHzd4F6		MoTyNlQhcHK\|	NEjKPXhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCjZoTldkAzPCCqcoOgZpkhS2WubGTpeIVzNUKudXXD[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE43\|ryP	M2HXbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUS1N\|M1Lz5{MEW0OVM{PDxxYU6=
MDA-MB-231	NX;2bXlHS3m2b4P0ZZRq[yCjY4Tpeol1gSCjc4PhfS=>		NIrqRpg{KGSjeYO=	NFOzOIdEgXSxc4TheIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB\|IHThfZMh[nlibXnjdo91cXSncjDhd5NigQ>?	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV7OEW1OUc,OjB3OUi1OVU9N2F-
MCF7	M4\FbmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MXmyOEBpenN?	NWrFcoxMS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVgvQc7:TR?=	NFHMc2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[wOVQ4OCd-MkC2NFU1PzB:L3G+
MDA-MB-231	Moi4R5l1d3SxeHnjbZR6KGG\|c3H5		MnfPNlQhcHK\|	Ml[1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTFyzszN	MlXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW0O\|AoRjJyNkC1OFcxRC:jPh?=
HEK293	NHzmbm5EgXSxdH;4bYNqfHliYYPzZZk>		NXfoSpRMOjRiaILz	M1v3dGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTFyzszN	M{LzcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkC1OFcxLz5{ME[wOVQ4ODxxYU6=
HeLa	MnrQR5l1d3SxeHnjbZR6KGG\|c3H5		NFvGO5YzPCCqcoO=	MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUKuOe69VQ>?	MnG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW0O\|AoRjJyNkC1OFcxRC:jPh?=
BT-12	MmjYdWhVWyCjc4PhfS=>			M3\IPZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?=	NX7u[ZRFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NlE1QTJpPkKwOlIyPDl{PD;hQi=>
HeLa	NVT0c3hwTnWwY4Tpc44h[XO\|YYm=	M1\T[lEhfU1?	NVfNVY1JPDhiaILz	MVTBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmVGFiY3XscJMh[2:neIDy[ZN{cW6pIFXSSU1GOWJvTIXjJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[g[ZN1emGmaX;sMYlv\HWlZXSgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZZQhOSC3TTDh[pRmeiB2ODDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	M121TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkKxOFkzLz5{ME[yNVQ6OjxxYU6=
MCF7	MmroR5l1d3SxeHnjbZR6KGG\|c3H5	NFP6Z\|Y3KHWP	NFrGRW86PiCqcoO=	NGfjcG9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[XRiNjD1UUBi\nSncjC5OkBpenNiYomgUXRVKGG\|c3H5	MlrGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MkG0PVIoRjJyNkKxOFkzRC:jPh?=
HeLa	Mni0SpVv[3Srb36gZZN{[Xl?			NYrYZnBzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiC5aXzkJJR6eGViRWLhcJBp[SCneIDy[ZN{\WRiaX6gbJVu[W5iSHXMZUBk\WyuczDjc{1mgHC{ZYPzbY5oKEWURTDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIITyZY5{[WO2aY\heIlwdiCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk>	NX71NJN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2OVk5ODFpPkKwOlU6QDBzPD;hQi=>
HeLa	NHjSZoZHfW6ldHnvckBie3OjeR?=			NFXyPZRVemGwc3HjeIl3[XSrb36gc4YhcHWvYX6ge4lt\CC2eYDlJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGi3bXHuJGhmVGFiY3XscJMh[29vZYjwdoV{e2mwZzDBVE0yKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=>	MkThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4NUm4NFEoRjJyNkW5PFAyRC:jPh?=
HeLa	MULGeY5kfGmxbjDhd5NigQ>?			M{LVUGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hf2muZDD0fZBmKEWUYnX0ZUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWOYTDj[YxteyClbz3lfJBz\XO\|aX7nJGVTTSCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	NX[xcYZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2OVk5ODFpPkKwOlU6QDBzPD;hQi=>
HeLa	MU\GeY5kfGmxbjDhd5NigQ>?			MUXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIIfpcIQhfHmyZTDFVoJmfGFiZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgZ48u\XiycnXzd4lv\yCDUD2xJIF{e2W\|c3XkJIF{KHS{YX7zZYN1cX[jdHnvckBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M2XVOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkW5PFAyLz5{ME[1PVgxOTxxYU6=
MCF7	MXrBcpRq[2GwY3XyJIF{e2G7			MYXBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDlfJBz\XO\|aX7nJIV{fHKxZ3XuJJJm[2WydH;yJIJ6KE2WVDDhd5NigSxiSVO1NF0yOS56zszN	NHTq[lM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm1NVA{PSd-MkC5OVExOzV:L3G+
MCF7	MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NVL5PFg5PDhiaILz	NXruWoRJSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE4xODNibl2gc4Yh\XO2cnHkbY9tKGGwZDCwMlAyKG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTDpckBxemW\|ZX7j[UBw\iB3JTDm[ZRidCCkb4\pcoUhe2W{dX2sJGdKPTB;Mj6yPVA5P87:TR?=	MmTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO\|UoRjJzNUGzNlc2RC:jPh?=
MCF7	M1ryfmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MlHHOFghcHK\|	NXvRVpVISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNE4xPiCwTTDv[kBme3S{YXTpc4wh[W6mIECuNFchdk1ib3[geIV{fG:\|dHXyc45mKGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7IHnuJJBz\XOnbnPlJI9nKG[ndHHsJIJwfmmwZTDz[ZJ2dSCjbnSgUpVU\XK3bTygS2k2OD1{Lkm1NVIy\|ryP	M4H3[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
MCF7	NYfFeIt[SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M2KwU\|Q5KGi{cx?=	NYTwfpFPSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5IhcW6ldXLheIVlKHerdHigNVAxKG6PIH;mJIV{fHKjZHnvcEBidmRiMD6wO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK\|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XliaX6gdJJme2WwY3Wgc4Yh\mW2YXygZo93cW6nIIPldpVuKGGwZDDOeXNmenWvLDDHTVUxRTVwNkKzOFHPxE1?	MkPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUOyO\|UoRjJzNUGzNlc2RC:jPh?=
NCI-ADR-RES	NHPTW\|VCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M1XvbFQ5KGi{cx?=	NIfQ[FNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDOR2kuSUSULWLFV{Bk\WyuczDpcoN2[mG2ZXSge4l1cCByLkCwN{BvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEGgcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFUmKG[ndHHsJIJwfmmwZTDz[ZJ2dSxiR1m1NF02Njd3NEVOwG0>	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
MCF12A	MXvBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NXTUUGJEPDhiaILz	MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wNFMhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkCxJI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kA2LSCoZYThcEBjd3[rbnWgd4VzfW1uIFfJOVA:PS55NUS0{txO	NFq5dFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
MCF12A	MnjERY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MljwOFghcHK\|	M4H3VGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF3DSlEzSSClZXzsd{BqdmO3YnH0[YQhf2m2aDCxNFAhdk1ib3[g[ZN1emGmaX;sJIFv\CByLkC3JI5OKG:oIITld5Rwe3Sncn;u[UBi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSCrbjDwdoV{\W6lZTDv[kBn\XSjbDDic5ZqdmVic3XyeY0h[W6mIF71V4VzfW1uIFfJOVA:PS55NUS0{txO	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
MCF12A	M1u5XWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M135N\|Q5KGi{cx?=	MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHXzeJJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCPQ1[xNmEh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC\|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF03NjlzOEOx{txO	NXPSVW04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
NCI-ADR-RES	MnTGRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NWDzeY92PDhiaILz	NYLjfXpFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBme3S{b3flckBz\WOncITvdk1l\W[rY3nlcpQhcHWvYX6gUmNKNUGGUj3SSXMh[2WubIOgbY5kfWKjdHXkJJdqfGhiMD6wOkBvVSCxZjDld5Rz[WSrb3ygZY5lKDBwMEegcm0hd2ZidHXzeI9{fGW{b37lJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJIZmfGGuIHLveolv\SC\|ZYL1cUBidmRiToXT[ZJ2dSxiR1m1NF06NjV2OUmz{txO	M4LQT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
LNCAP	MoflRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M{nJTlQ5KGi{cx?=	NVSyVoxnSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMUmNCWCClZXzsd{BmgHC{ZYPzbY5oKGGwZILv[4VvKHKnY3XweI9zKGmwY4XiZZRm\CC5aYToJFAvPiCwTTDv[kBme3S{YXTpc4wh[W6mIECuO{BvVSCxZjD0[ZN1d3O2ZYLvcoUh[W[2ZYKgOFghcHK\|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OTEQvF2=	NX[0WIhiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVMzPzVpPkKxOVE{Ojd3PD;hQi=>
PC3	M4DsfGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MmPWOFghcHK\|	MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHu[JJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCSQ{OgZ4VtdHNiaX7jeYJifGWmIIfpeIghOC54IH7NJI9nKGW\|dILh[IlwdCCjbnSgNE44KG6PIH;mJJRme3Sxc4Tldo9v\SCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OD1zMD65OlQ5\|ryP	NH;DSVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUWxN\|I4PSd-MkG1NVMzPzV:L3G+
NCI-ADR-RES	NEj3R25CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NGXZ[lE1QCCqcoO=	NGjvV3JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDOR2kuSUSULWLFV{Bk\WyuczDpcoN2[mG2ZXSge4l1cCBzMECgcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjB5IH7NJI9nKHSnc4Tvd5Rmem:wZTDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUBqdiCycnXz[Y5k\SCxZjDm[ZRidCCkb4\pcoUhe2W{dX2gZY5lKE63U3XyeY0tKEeLNUC9NVEvPDhzNd88US=>	M1HwPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGzNlc2Lz5{MUWxN\|I4PTxxYU6=
DU145	NEDFRXZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NHK3VIg1QCCqcoO=	MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHHu[JJw\2WwIILlZ4VxfG:{LXTl[olkcWWwdDDoeY1idiCGVUG0OUBk\WyuczDpcoN2[mG2ZXSge4l1cCByLk[gcm0hd2ZiZYP0doFlcW:uIHHu[EAxNjdibl2gc4YhfGW\|dH;zeIVzd26nIHHmeIVzKDR6IHjyd{BjgSC\|dXzmc5Jpd2SjbXnu[UBDKGG\|c3H5MEBIUTVyPUGzMlgxOzkQvF2=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzM{K3OUc,OjF3MUOyO\|U9N2F-
MCF7	NUTBUJViS3m2b4TvfIlkcXS7IHHzd4F6			NYO2N4U6S3m2b4TvfIlkcXS7IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDNWHMh[XO\|YYmsJGlEPTB;M{JOwG0>	M4nLXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkiwNFY1Lz5{MU[4NFA3PDxxYU6=
ER-positive MCF7	MnvBRY51cWW\|dILv[4VvcWNiYYPzZZk>	NGrYT2QzOCCwTR?=	MkLLOFghcHK\|	NXW2PXpVSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gSXIueG:\|aYTpeoUhVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKDF5YnX0ZUBme3S{YXTpc4wucW6mdXPl[EB{\WO{ZYTl[EBidGujbHnu[UBxcG:\|cHjheIF{\SCjY4Tpeol1gSCjdDCyNEBvVSCjZoTldkA1QCCqcoOgZpkheGixc4DoZU1tcWeqdDDy[ZBwenSncjDjbIVucWy3bXnu[ZNk\W6lZTDhd5NigQ>?	Mn\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEC4OVkoRjJzOECwPFU6RC:jPh?=
HEK293	MorzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NGLpfFg1QCCqcoO=	M1HEO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFXLNlk{KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUFOwG0>	MkGyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MCF7	NVH5O4M{SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		Mm\NOFghcHK\|	NYL1VZdVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIV5eHKnc4Ppcoch\XO2cn;n[Y4hemWlZYD0c5Ih[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNk41QM7:TR?=	NIjifmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi3NVgyOid-MkG4O\|E5OTJ:L3G+
MDA-MB-231	MkizRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NVfyVVJVPDhiaILz	M1zVfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[ZN1em:pZX6gdoVk\XC2b4Kt[IVncWOrZX70JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTF6LkpOwG0>	NXTrU5NwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O\|E5OTJpPkKxPFcyQDF{PD;hQi=>
Ishikawa	NXKwXHJzSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NES3b\|k1QCCqcoO=	MlXNRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BmgHC{ZYPzbY5oKGW\|dILv[4VvKHKnY3XweI9zKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlIvPc7:TR?=	M4[yWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEexPFEzLz5{MUi3NVgyOjxxYU6=
A673	NG\XOWhyUFSVIHHzd4F6			MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>?	MkPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2N{KwOFUoRjJ{NEeyNFQ2RC:jPh?=
HEK293	MWXGeY5kfGmxbjDhd5NigQ>?		NUnZcmh[OS53IHjydy=>	MXTEbZNxdGGlZX3lcpQhd2ZiW{PIYWxUTCCocn;tJIh2dWGwIEXIWFYhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT\|I6OyClZXzsd{Bi\nSncjCxMlUhcHK\|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaU2xMlA1Oc7:TR?=	NEjKUpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkWzO\|E2Oyd-MkK1N\|cyPTN:L3G+
AH109	MYfGeY5kfGmxbjDhd5NigQ>?		NYjCfmE1OjRiaILz	NVW1Uo9GSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoJmfGFicnXj[ZB1d3JiTFLEJIV5eHKnc4Pl[EBqdiC7ZXHzeEBCUDFyOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGmwdHXyZYN1cW:wIIfpeIghW1KFMTDh[pRmeiB{NDDodpMh[nliYXzwbIEu\2GuYXP0c5Nq\GG\|ZTDhd5NigSxiSVO1NF0yNjZ4zszN	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
AH109	M2rIdWZ2dmO2aX;uJIF{e2G7		NX62UlY3OjRiaILz	NYD2XHNSSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIILlZ4VxfG:{IFzCSEBmgHC{ZYPz[YQhcW5ieXXhd5QhSUhzMEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTldoFkfGmxbjD3bZRpKFOUQ{GgZYZ1\XJiMkSgbJJ{KGK7IHHsdIhiNWejbHHjeI9{cWSjc3WgZZN{[XluIFnDOVA:Oi53NN88US=>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ2N{KxO{c,OjJ4NEeyNVc9N2F-
MCF7	M3XxSGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NWr0bGk4OjRiaILz	M1nRSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvPTUQvF2=	NWq3T\|dHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
DLD1	MWHDfZRwfG:6aXPpeJkh[XO\|YYm=		NYDPcJJ3QTZiaILz	MnPxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSGxFOSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUSuO\|jPxE1?	Ml\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N\|kzRC:jPh?=
MCF7	NWOzTVRDS3m2b4TvfIlkcXS7IHHzd4F6		MlPhPVYhcHK\|	MlvaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUeuNe69VQ>?	NHHnbXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
NIH/3T3	M4HhcmN6fG:2b4jpZ4l1gSCjc4PhfS=>		M{LIZVk3KGi{cx?=	M4\JdWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9\|VEOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjJ4zszN	NUfqZlZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO\|Q6Ozl{PD;hQi=>
518A2	MmXJR5l1d3SxeHnjbZR6KGG\|c3H5		NXXMUnN6QTZiaILz	MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC1NVhCOiClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUeuOlLPxE1?	M4PaSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N\|kzLz5{Mke0PVM6OjxxYU6=
A2780	M{XqZWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NYS5UYJIQTZiaILz	NF:5Z49EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvPzgQvF2=	NYnPSFN6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO\|Q6Ozl{PD;hQi=>
Lipo	NI\CPFJEgXSxdH;4bYNqfHliYYPzZZk>		NE\GSpI6PiCqcoO=	MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDMbZBwKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTB;OD62OO69VQ>?	NIfWenA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
A253	MnHjR5l1d3SxeHnjbZR6KGG\|c3H5		NH3re\|E6PiCqcoO=	MojJR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI2OyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUiuPVLPxE1?	MoTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N\|kzRC:jPh?=
A549	NIrhemJEgXSxdH;4bYNqfHliYYPzZZk>		MnTlPVYhcHK\|	M4HtRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgPVYhcHK\|IHL5JHNTSiCjc4PhfUwhUUN3ME25MlY3\|ryP	MoG2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N\|kzRC:jPh?=
850C	M2nhOWN6fG:2b4jpZ4l1gSCjc4PhfS=>		M2DMUFk3KGi{cx?=	MoPLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gPFUxSyClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUGxMlA6\|ryP	NX3ZVlJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO\|Q6Ozl{PD;hQi=>
KIF5	M3zCcnRwgGmlaYT5JIF{e2G7		MYqyOEBpenN?	MorHWI95cWOrdImgZYdicW6\|dDDoeY1idiCNSV[1JINmdGy\|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEOnbHzUbZRmeiCEbIXlJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI{Njd3zszN	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd5NUS3OEc,OjJ5N{W0O\|Q9N2F-
MCF7	MlfCR5l1d3SxeHnjbZR6KGG\|c3H5		NWnPXVNkPzJiaILz	MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QyKGG\|c3H5JIlvKHC{ZYPlcoNmKG:oIEKwKUBHSlNuIFnDOVA:QC54zszN	NFTCTGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmwNVg6PSd-MkK5NFE5QTV:L3G+
HL60	NYPaR5dYS3m2b4TvfIlkcXS7IHHzd4F6		NISzRXc4OiCqcoO=	MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QyKGG\|c3H5JIlvKHC{ZYPlcoNmKG:oIEKwKUBHSlNuIFnDOVA:OTRwM988US=>	M4DBSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUCxPFk2Lz5{MkmwNVg6PTxxYU6=
C41 DE3	M1TkbWZ2dmO2aX;uJIF{e2G7	MlTqOlAhfU1?	M3jjOVExKG2rboO=	NV;kc\|JXUW6jY4TpeoF1cW:wIH;mJGN[WDKENjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGM1OSCGRUOgZ4VtdHNiYYPz[ZN{\WRiYYOgcY9lcW[rZXSgZZBwNVB2NUCgNmI3KGGmZIXjeEBnd3KvYYTpc44h[XRiNkCgeW0h[W[2ZYKgNVAhdWmwczDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEh?	NF;ncI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm0NlMyPyd-MkK5OFI{OTd:L3G+
C41 DE3	MYPGeY5kfGmxbjDhd5NigQ>?	MmfpOlAhfU1?		MVfJcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyCvb3Tp[olm\CCjcH:tVFQ2OCB{Qk[gZYRlfWO2IH\vdo1ifGmxbjDheEA3OCC3TTDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEhiYX7kJGdUUEWH	M4q3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN\|E4Lz5{Mkm0NlMyPzxxYU6=
C41 DE3	NVTOZXBqTnWwY4Tpc44h[XO\|YYm=	MlPmNVAxKHWP	M1HjPVMxKG2rboO=	MUDJcoFkfGm4YYTpc44hd2ZiQ2nQNmI3KCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQ{SxJGRGOyClZXzsd{Bie3Onc4Pl[EBieyC2conweIlkKHCncITp[IUhOTh6RljZVWRSTTF7NDD3bZRpKHOrdHWgc4Yh[WSmdXP0JIZwem2jdHnvckBtd2OjbHn6[YQhfG9iR3zuNVk{KGG2IEGwNEB2VSCjZoTldkA{OCCvaX7zJIJ6KEyFLV3TM21UKGGwYXz5d4l{KGmwIIDy[ZNmdmOnIH;mJG5CTFCK	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl2MkOxO{c,OjJ7NEKzNVc9N2F-
MCF7	MnjOR5l1d3SxeHnjbZR6KGG\|c3H5		MnrkOFghcHK\|	M2n2bWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNU41PM7:TR?=	NVfRWlA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlIzQDFpPkKzNFIzOjhzPD;hQi=>
MDA-MB-231	NH;5bZREgXSxdH;4bYNqfHliYYPzZZk>		MYS0PEBpenN?	M2D5TmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMj60Ne69VQ>?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{MkK4NUc,OjNyMkKyPFE9N2F-
MCF7	M1viPWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MoHVOFghcHK\|	NEn1UG5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUB{fWyob4Loc4RidWmwZTDCJIF{e2G7LDDJR\|UxRTFwOENOwG0>	M4rOeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MUKzNFE4Lz5{M{GyN\|AyPzxxYU6=
DU145	MX3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M330dlQ5KGi{cx?=	MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESXMUS1JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:PS53zszN	NF\rU\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
MCF7	M{TGVGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NFX5cpA1QCCqcoO=	M2PO[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTVwNd88US=>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6N{C1O{c,OjN{OEewOVc9N2F-
HepG2	Mmi0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NVqxd5Z3PDhiaILz	MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Pi5\|zszN	NXfXTod[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyPFcxPTdpPkKzNlg4ODV5PD;hQi=>
PC3	NWTOepZFSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MV20PEBpenN?	MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEx\|ryP	NInxT5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
NIH/3T3	MXXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NHjmWG41QCCqcoO=	M2jH[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViTlnIM\|NVOyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUGxMlTPxE1?	NFTjUGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
MDA-MB-231	NUXxe3JrSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NIWxTmE1QCCqcoO=	M3e0cWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVEyNjUQvF2=	M1qxb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki3NFU4Lz5{M{K4O\|A2PzxxYU6=
A549	MofiRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MoHOOFghcHK\|	MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOs7:TR?=	Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OEewOVcoRjJ\|Mki3NFU4RC:jPh?=
T47D	MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NXi1fmFwPDhiaILz	NUjlOoFoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUROwG0>	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6N{C1O{c,OjN{OEewOVc9N2F-
MCF7	M{Lod2N6fG:2b4jpZ4l1gSCjc4PhfS=>	M2joelUhfU1?	MYW0PEBpenN?	NGnBR4VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPoZY5o\SCrbjDj[YxtKH[rYXLpcIl1gSCjdDC1JJVOKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk>	NIHKXWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
HT-29	MmrGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?			M{X0fWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhVNTJ7IHPlcIx{NCCJSUWwQVkvPc7:TR?=	NVPabod{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFMxQDRpPkKzOFA{ODh2PD;hQi=>
M21	MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?			NFHvZZZIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOOjFiY3XscJMtKEeLNUC9NVHPxE1?	NV3XWVIxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFMxQDRpPkKzOFA{ODh2PD;hQi=>
MCF7	NF\QOnNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=			NYTUPXpZT3Kxd4ToJIlvcGmkaYTpc44hd2ZiZYP0do9o\W5vZHXw[Y5l\W62IHj1cYFvKE2FRkegZ4VtdHNuIFfJOVA:OTFwMt88US=>	M3yzblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NECzNFg1Lz5{M{SwN\|A5PDxxYU6=
MDA-MB-231	MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?			M1n4cGdzd3e2aDDpcohq[mm2aX;uJI9nKGW\|dILv[4VvNWmwZHXw[Y5l\W62IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMtKEeLNUC9NVgvQM7:TR?=	MonvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEOwPFQoRjJ\|NECzNFg1RC:jPh?=
HL60	M2PXW2Z2dmO2aX;uJIF{e2G7			MWnJcohq[mm2aX;uJI9nKESnbIThLFgqNUSnbIThLFcqKHO2ZYLvcEBqe2:vZYLhd4UwTGWudHGoNlQqNXO2ZYLvcEBz\WS3Y4Thd4UudWWmaXH0[YQh[2ixbHXzeIVzd2xiYnnvd5lvfGinc3nzJIlvKGi3bXHuJGhNPjBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gfplud3O2ZX7vcEBidmRieontc5N1\XKxbDDhZ4N2dXWuYYTpc44>	M4nLfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEOzOlY4Lz5{M{SzN\|Y3PzxxYU6=
MCF7	NYTMPGZtTnWwY4Tpc44h[XO\|YYm=		NYXDOlVbPiCmYYnz	MW\BcpRi\2:waYP0JIFkfGm4aYT5JIF1KEWUYXzwbIEhemWlZYD0c5IhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHHmeIVzKDZiZHH5d{BjgSClconzeIFtKH[rb3zleEB{fGGrbnnu[{Bu\XSqb3SsJGlEPTB;MD6y{txO	Mon1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEizOFYoRjJ\|NES4N\|Q3RC:jPh?=
MCF7	MVnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		Mli4O\|IhcHK\|	NILDWoRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IITyfZBidiCkbIXlJIF{e2G7LDDHTVUxRTF{zszN	NH7QXm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ezOVgzQSd-MkO3N\|U5Ojl:L3G+
MSTO-211H	NX[0fIFySW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M4\WU\|czKGi{cx?=	MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2VVF:tNlEyUCClZXzsd{Bi\nSncjC3NkBpenNiYomgeJJ6eGGwIHLseYUh[XO\|YYmsJGdKPTB;MkOuN:69VQ>?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd\|NUiyPUc,OjN5M{W4Nlk9N2F-
HeLa	MUXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		Mkn3O\|IhcHK\|	MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHS{eYDhckBjdHWnIHHzd4F6NCCJSUWwQVMzNjcQvF2=	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd\|NUiyPUc,OjN5M{W4Nlk9N2F-
HCT116	NXSxSlM3TnWwY4Tpc44h[XO\|YYm=	NVrrOWV5OC53IH3N	NUHFNYdrOjRiaILz	Mlf4SYZn\WO2IH;uJI1qfG:laH;u[JJq[WxicnXzdIlz[XSrb36gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGW6cILld5NqdmdiY3HyZo9vcWNiYX7ofYRz[XOnIEmgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIH;4fYdmdiClb37zeY1xfGmxbjDyZZRmKGG2IECuOUBuVSCjZoTldkAzPCCqcoOgeY5l\XJiaInwc5hq[yClb37kbZRqd25iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{	MlLTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OE[0OVIoRjJ\|N{i2OFUzRC:jPh?=
MCF7	MVLDfZRwfG:6aXPpeJkh[XO\|YYm=		NID0W2E4OiCqcoO=	M3zvc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh\XiycnXzd4lv\yCHUnHsdIhiKGGwZDDFVoJmfGFiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zNT62{txO	NFS3epk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
Vero	MXHDfZRwfG:6aXPpeJkh[XO\|YYm=		M2\nc\|czKGi{cx?=	NV3zcGV4S3m2b4TvfIlkcXS7IHHnZYlve3RiYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE2NjoQvF2=	M4DBTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{i2OFUzLz5{M{e4OlQ2OjxxYU6=
MDA-MB-231	M2PQVGN6fG:2b4jpZ4l1gSCjc4PhfS=>		Moe5O\|IhcHK\|	NGf0N2REgXSxdH;4bYNqfHliYXfhbY5{fCCHUnHsdIhiKGGwZDDFVoJmfGFvZHXmbYNq\W62IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yP87:TR?=	NEnB[YM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
DU145	M33yfmN6fG:2b4jpZ4l1gSCjc4PhfS=>		M{PIelczKGi{cx?=	M2fRZmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEWUYXzwbIEu\GWoaXPp[Y51KGi3bXHuJGRWOTR3IHPlcIx{KGW6cILld5NqdmdiRWLi[ZRiKGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OjhwOd88US=>	MlGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OE[0OVIoRjJ\|N{i2OFUzRC:jPh?=
Jurkat	Mn7FR5l1d3SxeHnjbZR6KGG\|c3H5		MnjMOFghcHK\|	NHnuNmJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBLfXKtYYSgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF05NjQQvF2=	Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUKzOVIoRjJ\|N{myN\|UzRC:jPh?=
MCF7	MXrDfZRwfG:6aXPpeJkh[XO\|YYm=			NXK3VphvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDTVmIh[XO\|YYmsJGlEPTB;NT6xPe69VQ>?	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzhyNkCxOEc,OjN6ME[wNVQ9N2F-
MCF7	NHHY[YtCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NGrhR2E1QCCqcoO=	MoHERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTJwM{ZOwG0>	Mn6zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6M{C1NFMoRjJ\|OEOwOVA{RC:jPh?=
MCF7	NFy2RnJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		Mn;1O\|IhcHK\|	M2H5eGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTJ5Lkm2{txO	NVy5TodKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlA2QTJpPkKzPFYxPTl{PD;hQi=>
MCF7	NVz1O3p3S3m2b4TvfIlkcXS7IHHzd4F6		MonNOUBl[Xm\|	NYHkVJliS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA2KGSjeYOsJGlEPTB;MD63PVTPxE1?	M3T2cFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OE[0PVI5Lz5{M{i2OFkzQDxxYU6=
T47D	NG\0NWpEgXSxdH;4bYNqfHliYYPzZZk>		NWXMW4FQPSCmYYnz	M2fZSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHQ1P0RiY3XscJMh[W[2ZYKgOUBl[Xm\|LDDJR\|UxRTFwMUROwG0>	MnnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NkS5NlgoRjJ\|OE[0PVI5RC:jPh?=
MCF7	M3XjZmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NGjVXos1QCCqcoO=	NH\zWIVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVUvOs7:TR?=	MnrOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6OECzOVkoRjJ\|OEiwN\|U6RC:jPh?=
MCF7	MkLURY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NUm5NWtsPDhiaILz	NGHFS\|VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zMz60Oe69VQ>?	M{HMN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUCyPFA1Lz5{M{mwNlgxPDxxYU6=
ScN2a-cl3	M4fjbmZ2dmO2aX;uJIF{e2G7		NYHLW4RLPSCmYYnz	MnTUTY5pcWKrdHnvckBw\iCUTVygdJJqd25icILveIVqdiCrbn\lZ5Rm\CCrbjDtc5V{\SCmaY\p[Ilv\yCVY16yZU1kdDNiY3XscJMh\XiycnXzd4lv\yCodXzsJIxmdme2aDDtc5V{\SCScmCgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKG:oIGDyVJNkKGyndnXsJIFnfGW{IEWg[IF6eyCkeTDFUGlUSSxiRVO1NF01NjN7zszN	NXnTPXBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxPFM2QDlpPkK0NVg{PTh7PD;hQi=>
ScN2a-cl3	MmThR5l1d3SxeHnjbZR6KGG\|c3H5		M1HteVUh\GG7cx?=	NVLTOm5US3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4Uh\Gm4aXTpcochW2OQMnGtZ4w{KGOnbHzzJIF{e2W\|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IEWg[IF6eyCkeTDjZYxk\WmwLVHNJJN1[WmwaX7nMYJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygUGM2OD12Lki5{txO	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6M{W4PUc,OjRzOEO1PFk9N2F-
MCF7	MoDtR5l1d3SxeHnjbZR6KGG\|c3H5		NYq5[lRvOjRiaILz	MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;Od88US=>	MmDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUewPVQoRjJ2NEW3NFk1RC:jPh?=
MDA-MB-231	M3fTSmN6fG:2b4jpZ4l1gSCjc4PhfS=>		NUHrOHBwOjRiaILz	MljjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTFyzszN	MorSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUewPVQoRjJ2NEW3NFk1RC:jPh?=
HEK293	MnnGR5l1d3SxeHnjbZR6KGG\|c3H5		NYXxR215OjRiaILz	NGDMPXhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zPs7:TR?=	MnrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUewPVQoRjJ2NEW3NFk1RC:jPh?=
MCF7	M4XLeWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NFS1NmI1QCCqcoO=	M4PBW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTFwOEpOwG0>	NEnjbZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEeyNVczPyd-MkS3NlE4Ojd:L3G+
MCF7	NH;Vd2lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M1TOTVI1KGi{cx?=	M4r2fmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTcQvF2=	MkfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OECyN\|AoRjJ2OEiwNlMxRC:jPh?=
PC3	MXrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MofhNlQhcHK\|	MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVfPxE1?	MoDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6OECyN\|AoRjJ2OEiwNlMxRC:jPh?=
MDA-MB-231	Ml35RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MUWyOEBpenN?	MoHXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVTPxE1?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh6MEKzNEc,OjR6OECyN\|A9N2F-
HeLa	NWK3bXZZS3m2b4TvfIlkcXS7IHHzd4F6	MnLHNVAhdU1?	NHLHUIk1QCCqcoO=	MnP4R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliaX6gdJJme2WwY3Wgc4YhOTBibV2gUmFEKGGodHXyJFQ5KGi{czDifUBYW1R6IHHzd4F6	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4M{[2O{c,OjVzNkO2Olc9N2F-
MCF7	MmW0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		Mnv3NkBl[Xm\|	MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idiCHUj3wc5NqfGm4ZTDNR2Y4KGOnbHzzJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO\|YYmsJGlEPTB;ND62O:69VQ>?	M4LPSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MkKyPFc3Lz5{NUKyNlg4PjxxYU6=
MIAPaCa2	M3nLZ2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		MXuyJIRigXN?	MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idiCPSVHQZWNiOiClZXzsd{Bi\nSncjCyJIRigXNiYomgUXRVKGG\|c3H5MEBKSzVyPUOzMlEz\|ryP	NFztdWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUKyNlg4Pid-MkWyNlI5PzZ:L3G+
MDA-MB-231	Ml32RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NH31N3EzKGSjeYO=	NX;2Zo9uSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcHWvYX6gSXIudmWpYYTpeoUhVUSDLV3CMVI{OSClZXzsd{Bi\nSncjCyJIRigXNiYomgUXRVKGG\|c3H5MEBKSzVyPUO4Mlk4\|ryP	MlTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV{MkK4O\|YoRjJ3MkKyPFc3RC:jPh?=
MCF7	MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M3GyN\|czKGi{cx?=	MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01NjIQvF2=	MlzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV\|NkmzOlcoRjJ3M{[5N\|Y4RC:jPh?=
K562	NHjUeVBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NV;YfJUxPzJiaILz	MWPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhPzJiaILzMEBIUTVyPUWuNu69VQ>?	Mn:5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2MkCxO\|UoRjJ3NEKwNVc2RC:jPh?=
MCF7	MXjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NHvvd4w2KGSjeYO=	MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPSCmYYnzJIJ6KFeVVD24JIF{e2G7LDDJR\|UxRTJwMd88US=>	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzNEGxPEc,OjV4MUSxNVg9N2F-
MCF7	NILmcllCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NX\TPHZ7OyCmYYnz	MoTLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJIdzd3e2aDDpcoN2[mG2ZXSgZZQhOzdiZHXnR{Bnd3JiMzDkZZl{KGK7IILld4F7fXKrbjDtbYNzd3CuYYTlJIF{e2G7LDDJR\|UxRTF6Lkex{txO	M3n1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0OlI{Lz5{NUezOFYzOzxxYU6=
MCF7	NFvxb\|JEgXSxdH;4bYNqfHliYYPzZZk>			MmLpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBjgSCPVGSgZZN{[XluIFnDOVA:Oy56zszN	NF\qVmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC3PVA6OCd-Mk[wO\|kxQTB:L3G+
MDA-MB-435S	NGXPdolEgXSxdH;4bYNqfHliYYPzZZk>			NEXSUnhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtOFM2WyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBjgSCPVGSgZZN{[XluIFnDOVA:PS53zszN	M2jqNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEe5NFkxLz5{NkC3PVA6ODxxYU6=
Vero	M2TybmN6fG:2b4jpZ4l1gSCjc4PhfS=>			MoHER5l1d3SxeHnjbZR6KGGpYXnud5QhSW[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDifUBOXFRiYYPzZZktKEmFNUC9OU44\|ryP	M17vSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEe5NFkxLz5{NkC3PVA6ODxxYU6=
A549	NYPqOFRLS3m2b4TvfIlkcXS7IHHzd4F6			MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W\|c3XkJIF{KGOnbHygeoli[mmuaYT5JIJ6KE2WVDDhd5NigSxiSVO1NF05NjUQvF2=	Mnn3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyN{mwPVAoRjJ4MEe5NFkxRC:jPh?=
MCF7	MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NIm4elE1QCCqcoO=	M2HWfGFvfGlvcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTkQvF2=	NUfCdZBXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOlMzOjBpPkK2NVY{OjJyPD;hQi=>
DU145	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NXPkTVZUPDhiaILz	MX\BcpRqNXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCGVUG0OUBk\WyuczDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OTlwM988US=>	MnXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNkOyNlAoRjJ4MU[zNlIxRC:jPh?=
HepG2	NEe1RZZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M1fkdlQ5KGi{cx?=	MnznRY51cS2ycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwS\|Ih[2WubIOgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxRTJzLkhOwG0>	NWCwbId5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOlMzOjBpPkK2NVY{OjJyPD;hQi=>
A549	MnfHRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MV60PEBpenN?	NWnLTlZlSW62aT3wdo9tcW[ncnH0bZZmKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDTVmIh[XO\|YYmsJGdKPTB;MkSuOO69VQ>?	M4\WNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MU[zNlIxLz5{NkG2N\|IzODxxYU6=
GBM2	MnXPRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		Mn[5O\|IhcHK\|	NIHiTY5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFfCUVIh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO\|IhcHK\|IHL5JHdUXC1zIH3leIhw\CxiSVO1NF0{NjYQvF2=	MoDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|NUW1N\|IoRjJ4M{W1OVMzRC:jPh?=
GBM3	M3nlR2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		MYG3NkBpenN?	M3q4[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iR1LNN{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC3NkBpenNiYomgW3NVNTFibXX0bI9lNCCLQ{WwQVQvP87:TR?=	MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ\|NUW1N\|IoRjJ4M{W1OVMzRC:jPh?=
GBM1	MmrRRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MUW3NkBpenN?	NWDWWYJXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDHRm0yKGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBYW1RvMTDt[ZRpd2RuIFnDOVA:Py53zszN	NWLUVHh4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zOVU2OzJpPkK2N\|U2PTN{PD;hQi=>
MCF7	M3PhN2FvfGmycn;sbYZmemG2aY\lJIF{e2G7			M{K4cGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczygTWM2OD1yLkC1O\|U1\|ryP	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjRyN{CxNkc,OjZ2MEewNVI9N2F-
MCF	M1HXTmZ2dmO2aX;uJIF{e2G7			Mk\QRY51[WexbnnzeEBi[3Srdnn0fUBifCCycn;n[ZN1\XKxbnWgdoVk\XC2b4KgbY4hcHWvYX6gUWNHKGOnbHzzJIF{e2W\|c3XkJIF{KGW\|dILh[IlwdC2rbnT1Z4VlKHKnY3XweI9zKHKnc4DvcpNmNCCLQ{WwQVAvOTJ6OENOwG0>	NXzGcnc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[0NFcxOTJpPkK2OFA4ODF{PD;hQi=>
MCF7	NYrBb5prTnWwY4Tpc44h[XO\|YYm=			NWHycYt2SW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIILlZ4VxfG:{IHnuJIh2dWGwIF3DSlch[2WubIOsJGlEPTB;MUFOwG0>	Mo\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ2MEewNVIoRjJ4NEC3NFEzRC:jPh?=
Neuro2a	MkTaSpVv[3Srb36gZZN{[Xl?	NW\ZepNjOSC3TR?=		M4riNmlvcGmkaYTpc44hd2ZiZHXseIEhQC15IHnzc41memG\|ZTDpckBud3W\|ZTDO[ZVzdzKjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJFcuTEiFIHzleoVteyCjdDCxJJVOKGK7IFzDMW1UN0eFLV3TJIFv[Wy7c3nz	NVHre3d6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O\|g6PjV5PD;hQi=>
Neuro2a	NF62epdHfW6ldHnvckBie3OjeR?=	M{DLUFEhfU1?		M2XpTGlvcGmkaYTpc44hd2ZiZHXseIEhQC15IHnzc41memG\|ZTDpckBFcGO{Nz3k[YZq[2mnboSgcY92e2ViTnX1do8z[SClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjD6fY1we3Snbn;sJIxmfmWuczDheEAyKHWPIHL5JGxENU2VL1fDMW1UKGGwYXz5d4l{	NVHEPHNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O\|g6PjV5PD;hQi=>
Neuro2a	M3XBcmZ2dmO2aX;uJIF{e2G7	MVGxJJVO		MlrHTY5pcWKrdHnvckBw\iCmZXz0ZUA5NTdiaYPvcYVz[XOnIHnuJGRp[3J5LXTl[olkcWWwdDDtc5V{\SCQZYXyc\|JiKGOnbHzzJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIIr5cY9{fGW{b3ygcIV3\Wy\|IHH0JFEhfU1iYomgUGMuVVNxR1OtUXMh[W6jbInzbZM>	M{LwU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{i5OlU4Lz5{Nke4PVY2PzxxYU6=
Neuro2a	MUfGeY5kfGmxbjDhd5NigQ>?	M4ixe\|EhfU1?		NVixdGViUW6qaXLpeIlwdiCxZjDk[Yx1[SB6LUegbZNwdWW{YYPlJIlvKESqY4K3MYRm\mmlaXXueEBud3W\|ZTDO[ZVzdzKjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHTlZ5Jm[XOnIHnuJINpd2ync4Tldo9tKGyndnXsd{BifCBzIIXNJIJ6KEyFLV3TM2dENU2VIHHuZYx6e2m\|	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd6OU[1O{c,OjZ5OEm2OVc9N2F-
Neuro2a	NI\MV4VHfW6ldHnvckBie3OjeR?=	MWqxJJVO		NIHUeItKdmirYnn0bY9vKG:oIHTlcJRiKDhvNzDpd49u\XKjc3WgbY4hTGilcket[IVncWOrZX70JI1wfXOnIF7leZJwOmFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gO{1FUENibHX2[Yx{KGG2IEGgeW0h[nliTFOtUXMwT0NvTWOgZY5idHm\|aYO=	M1vGe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{i5OlU4Lz5{Nke4PVY2PzxxYU6=
Neuro2a	NI\wNIFHfW6ldHnvckBie3OjeR?=	NGLvZWwyKHWP		MXrJcohq[mm2aX;uJI9nKESUMkSgbY4hTGilcket[IVncWOrZX70JI1wfXOnIF7leZJwOmFiY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gO{1FUENibHX2[Yx{KGG2IEGgeW0h[nliTFOtUXMwT0NvTWOgZY5idHm\|aYO=	NFvpTVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke4PVY2Pyd-Mk[3PFk3PTd:L3G+
Neuro2a	NFvDVJhHfW6ldHnvckBie3OjeR?=	M{LxflEhfU1?		NV3pPI1TUW6qaXLpeIlwdiCxZjDEVlI1KGmwIH3veZNmKE6ndYLvNoEh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iNz3ETGMhdGW4ZXzzJIF1KDFidV2gZpkhVENvTWOvS2MuVVNiYX7hcJl{cXN?	NU\aNHYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3PFk3PTdpPkK2O\|g6PjV5PD;hQi=>
AsPC1	NEDBNFVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	NVe0UVd3OTBidV2=	M4D1S\|Q5KGi{cx?=	NWL5UXQ2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBd3BEOSClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBifCBzMDD1UUBi\nSncjC0PEBpenNiYomgUXRUKGG\|c3H5	NUTpRXhFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4NFMzODRpPkK2PFA{OjB2PD;hQi=>
BxPC3	NEHBN\|ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	MmHNNVAhfU1?	MkLuOFghcHK\|	NVPCXJJKSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCfHBEOyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBifCBzMDD1UUBi\nSncjC0PEBpenNiYomgUXRUKGG\|c3H5	NHL1Tng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkiwN\|IxPCd-Mk[4NFMzODR:L3G+
MIAPaCa2	NHvF[3FCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=	MWGxNEB2VQ>?	MmfFOFghcHK\|	MlPwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPSVHQZWNiOiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBifCBzMDD1UUBi\nSncjC0PEBpenNiYomgUXRUKGG\|c3H5	M3PBVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OECzNlA1Lz5{NkiwN\|IxPDxxYU6=
MCF7	M3:0dWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NEnDVFU1QCCqcoO=	MkH1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVEvPTkQvF2=	NVjWeZpXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4PVY4ODZpPkK2PFk3PzB4PD;hQi=>
T47D	Mnu3R5l1d3SxeHnjbZR6KGG\|c3H5		MlLJOFghcHK\|	NImxUohEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:OS57Od88US=>	MnPEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6OU[3NFYoRjJ4OEm2O\|A3RC:jPh?=
MOLT4	Mk\WR5l1d3SxeHnjbZR6KGG\|c3H5		Mlu5OFghcHK\|	MlzjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW9NXDRiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0zNjYQvF2=	NGHMT2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki5OlcxPid-Mk[4PVY4ODZ:L3G+
MDA-MB-468	NGXSVHlEgXSxdH;4bYNqfHliYYPzZZk>		NIDqN5M1QCCqcoO=	Ml3NR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUS2PEBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC0PEBpenNiYomgV3JDKGG\|c3H5MEBIUTVyPUKuOe69VQ>?	NITpZo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki5OlcxPid-Mk[4PVY4ODZ:L3G+
HCT15	NIP3cYhEgXSxdH;4bYNqfHliYYPzZZk>		MWq0PEBpenN?	MkfzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTViY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0{NjF4zszN	MlvQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6OU[3NFYoRjJ4OEm2O\|A3RC:jPh?=
MDA-MB-435	NFqz[IFEgXSxdH;4bYNqfHliYYPzZZk>		M{D4cFQ5KGi{cx?=	NXW5WodXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVMvOTcQvF2=	NGHCOo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nki5OlcxPid-Mk[4PVY4ODZ:L3G+
MCF7	MUDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M3PNeFczKGi{cx?=	M321dWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTRwNN88US=>	M3zGSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEm2O\|A3Lz5{Nki5OlcxPjxxYU6=
SNB75	M3jj[GN6fG:2b4jpZ4l1gSCjc4PhfS=>		NXf6VlZvPDhiaILz	MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTUmI4PSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCJSUWwQVUvODIQvF2=	MmDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6OU[3NFYoRjJ4OEm2O\|A3RC:jPh?=
EKVX	MX7DfZRwfG:6aXPpeJkh[XO\|YYm=		M{CwXlQ5KGi{cx?=	NHnqVYhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGU1[[IHPlcIx{KGG\|c3Xzd4VlKGG\|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVA:Pi5\|zszN	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh7NkewOkc,OjZ6OU[3NFY9N2F-
UO31	NYDSeGJFS3m2b4TvfIlkcXS7IHHzd4F6		Mkj0OFghcHK\|	M4OyTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVQOzFiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF03NjQQvF2=	NV\ZWppzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4PVY4ODZpPkK2PFk3PzB4PD;hQi=>
SKOV3	MonWR5l1d3SxeHnjbZR6KGG\|c3H5		MWW0PEBpenN?	MonpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiR1m1NF0yOM7:TR?=	NYXjdWdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4PVY4ODZpPkK2PFk3PzB4PD;hQi=>
MRC5	Mn3sR5l1d3SxeHnjbZR6KGG\|c3H5		MnTpOEB1dyB5IHThfZM>	MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzJIF{e2W\|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IESgeI8hPyCmYYnzJIJ6KEGuYX3hdkBDdHWnIIP0ZYlvcW6pIHLhd4VlKG[udX;yc41mfHKrYzDhcoFtgXOrczygTWM2OD1zMD65{txO	M2rUbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUKyNlI3Lz5{NkmyNlIzPjxxYU6=
MCF7	Mke2SpVv[3Srb36gZZN{[Xl?	MV2zJJRwKDFyIIXN	M1jNRVE5KGi{cx?=	MVrJcohq[mm2aX;uJI9nKFOndEevPUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IIP1dJBz\XO\|aX;uJI9nKGKndHGt[ZN1emGmaX;sMYlv\HWlZXSgSXJGKHC{b33veIVzKGGldHn2ZZRqd25iYYSgN{B1dyBzMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFE5KGi{czDmc4xtd3enZDDifUBj\XSjLXXzeJJi\GmxbDDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	MlnqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyOEi2OFgoRjJ5MEi4OlQ5RC:jPh?=
MCF7	NUjyXllLTnWwY4Tpc44h[XO\|YYm=	MVqzJJRwKDFyIIXN	M2HwPFE5KGi{cx?=	NIHzXZlKdmirYnn0bY9vKG:oIGPleFcwQSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIHLleIEu\XO2cnHkbY9tNWmwZIXj[YQheFN{IH3SUmEh\XiycnXzd4lwdiCjdDCzJJRwKDFyIIXNJJBz\WmwY4XiZZRm\CCob4KgNVghcHK\|IH\vcIxwf2WmIHL5JIJmfGFvZYP0doFlcW:uIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM>	NYfme2RmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewPFg3PDhpPkK3NFg5PjR6PD;hQi=>
MCF7	MoK3RY51cWW\|dILv[4VvcWNiYYPzZZk>		NWrKbWU6QTZiaILz	NGTq[3FCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGOxbYDveY5lKGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgeI8h\GWlcnXhd4UhOTByIIDNJGUzNWmwZIXj[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHXxeYl3[WynboSgcIV3\Wy\|IHnu[JVk\WRiYomgOVAheE1iRUKgZYZ1\XJiOU[gbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlHPxE1?	M1zDT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUGwOlM2Lz5{N{GxNFY{PTxxYU6=
T47D	M2ToTWFvfGmnc4Tyc4dmdmmlIHHzd4F6		NI\6eIg6PiCqcoO=	NEnXXZhCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDUOFdFKGOnbHzzJIF{e2W\|c3XkJIF{KGOxbYDveY5lKGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgeI8h\GWlcnXhd4UhOTByIIDNJGUzNWmwZIXj[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHXxeYl3[WynboSgcIV3\Wy\|IHnu[JVk\WRiYomgOVAheE1iRUKgZYZ1\XJiOU[gbJJ{KGK7IHHsZY1ieiCkbIXlJIF{e2G7LDDBZ5Rqfmm2eU2wMlHPxE1?	M4OyWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUGwOlM2Lz5{N{GxNFY{PTxxYU6=
MCF7	MVLBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		NWX5TmFWQTZiaILz	MV3BcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJINwdXCxdX7kJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:g[IVkemWjc3WgNVAxKHCPIFWyMYlv\HWlZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGWzdXn2ZYxmdnRibHX2[Yx{KGmwZIXj[YQh[nliMUCgdG0hTTJiYX\0[ZIhQTZiaILzJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkROwG0>	NG\SUYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxNFY{PSd-MkexNVA3OzV:L3G+
T47D	NYTnWphrSW62aXXzeJJw\2WwaXOgZZN{[Xl?		M{\STVk3KGi{cx?=	NU\jOHJ1SW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gWFQ4TCClZXzsd{Bie3Onc4Pl[EBieyClb33wc5Vv\CClb37j[Y51emG2aX;uJJJmeXWrcnXkJJRwKGSnY4LlZZNmKDFyMDDwUUBGOi2rbnT1Z4VlKGOnbHygdJJwdGmoZYLheIlwdiC2bzDldZVqfmGuZX70JIxmfmWuczDpcoR2[2WmIHL5JFExKHCPIFWyJIFnfGW{IEm2JIhzeyCkeTDhcIFu[XJiYnz1[UBie3OjeTygRYN1cX[rdIm9NE46\|ryP	NVPFbFgzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVA3OzVpPkK3NVExPjN3PD;hQi=>
MCF7	MWXBcpRq\XO2cn;n[Y5q[yCjc4PhfS=>		NGjMWno6PiCqcoO=	MXXBcpRq\XO2cn;n[Y5q[yCjY4Tpeol1gSCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJINwdXCxdX7kJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:g[IVkemWjc3WgNVAxKHCPIFWyMYlv\HWlZXSgZ4VtdCCycn;sbYZmemG2aX;uJJRwKGWzdXn2ZYxmdnRibHX2[Yx{KGmwZIXj[YQh[nliMTDwUUBGOiCjZoTldkA6PiCqcoOgZpkh[WyjbXHyJIJtfWViYYPzZZktKEGldHn2bZR6RTRwNd88US=>	NXfqcWxPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVA3OzVpPkK3NVExPjN3PD;hQi=>
T47D	NE\zXopCdnSrZYP0do9o\W6rYzDhd5NigQ>?		NFrtOY06PiCqcoO=	NHG1V4pCdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDUOFdFKGOnbHzzJIF{e2W\|c3XkJIF{KGOxbYDveY5lKGOxbnPlcpRz[XSrb36gdoVyfWm{ZXSgeI8h\GWlcnXhd4UhOTByIIDNJGUzNWmwZIXj[YQh[2WubDDwdo9tcW[ncnH0bY9vKHSxIHXxeYl3[WynboSgcIV3\Wy\|IHnu[JVk\WRiYomgNUBxVSCHMjDh[pRmeiB7NjDodpMh[nliYXzhcYFzKGKudXWgZZN{[XluIFHjeIl3cXS7PUhOwG0>	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzME[zOUc,OjdzMUC2N\|U9N2F-
MCF7	NILDcW9EgXSxdH;4bYNqfHliYYPzZZk>		NV3GW4ZnPzJiaILz	MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGOnbHyg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IITyfZBidiCkbIXlJIF{e2G7LDDHTVUxRTZwNd88US=>	NY[wOGxjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNlgyPzVpPkK3NVI5OTd3PD;hQi=>
HeLa	NEPCOplEgXSxdH;4bYNqfHliYYPzZZk>		M4HXPFczKGi{cx?=	NGTkcHFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JJRzgXCjbjDicJVmKGG\|c3H5MEBIUTVyPUGy{txO	M2LB[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUK4NVc2Lz5{N{GyPFE4PTxxYU6=
A2780	Mnv6R5l1d3SxeHnjbZR6KGG\|c3H5		MlLlO\|IhcHK\|	M4L3T2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGG\|c3Xzd4VlKGG\|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JG1VWyCjc4PhfUwhT0l3ME2zNE4{\|ryP	NGWwfVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GyPFE4PSd-MkexNlgyPzV:L3G+
OVCAR5	M12xUGN6fG:2b4jpZ4l1gSCjc4PhfS=>		MUW3NkBpenN?	MoHaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gU3ZESVJ3IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgO\|IhcHK\|IHL5JG1VWyCjc4PhfUwhT0l3ME2zNu69VQ>?	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF{OEG3OUc,OjdzMkixO\|U9N2F-
MCF7	M2\WcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7			MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNuIFnDOVA:OTNwN988US=>	MkP0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW0OlkoRjJ5MUW1OFY6RC:jPh?=
MDA-MB-231	NXPDeWh5SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=			MnPrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{NCCLQ{WwQVE4NjoQvF2=	Mk\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW0OlkoRjJ5MUW1OFY6RC:jPh?=
MCF7	M{jWemFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MlP0OFghcHK\|	MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiaX7jeYJifGWmIH\vdkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVUvO87:TR?=	M1fPSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUe2PVQ1Lz5{N{G3Olk1PDxxYU6=
Ishikawa	NWDOdGdUSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M2fVNFQ5KGi{cx?=	NGHVdoZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFnzbIls[XejIHPlcIx{KGmwY4XiZZRm\CCob4KgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME22{txO	M33uVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUe2PVQ1Lz5{N{G3Olk1PDxxYU6=
MDA-MB-231	NXjmNmp5SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MV[0PEBpenN?	NWDFWo15SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOy57zszN	NIK4bow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G3Olk1PCd-MkexO\|Y6PDR:L3G+
MCF7	NELvR4VEgXSxdH;4bYNqfHliYYPzZZk>			NY\uT244S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDy[ZNignW{aX6gcYlkem:ybHH0[UBie3OjeTygTWM2OD14Lkm5{txO	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ{OEG1PUc,Ojd{MkixOVk9N2F-
MCF7	NFTtXG1EgXSxdH;4bYNqfHliYYPzZZk>			NEXkWWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDifUBz\XOjeoXybY4hdWmlcn;wcIF1\SCjc4PhfUwhUUN3ME2xPU4{O87:TR?=	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzNzMUi5OEc,Ojd\|MUG4PVQ9N2F-
MCF7	NUfkdVJxSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MVi3NkBpenN?	NG\yeVRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVTNXCxc3n0bZZmKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IH3lZZN2emWmIHHmeIVzKDd{IHjyd{BjgSCDbHHtZZJDdHWnIHHzd4F6NCCLQ{WwQVQvOc7:TR?=	MofpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd2MEewN\|AoRjJ5NEC3NFMxRC:jPh?=
insect cells	NYrVWlJPTnWwY4Tpc44h[XO\|YYm=			NHHX[YdFcXOybHHj[Y1mdnRib3[gSox2d3Kvb37lJGVUOiCJcnXlckBnem:vIHj1cYFvKHKnY3;tZolv[W62IH\1cIwhdGWwZ4ToJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKGmwc3XjeEBk\WyuczDt[YF{fXKnZDD1dEB1dyB2IHjyd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5MEBKSzVyPUCuNlIz\|ryP	M2L2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N\|c2Lz5{N{[0O\|M4PTxxYU6=
SH-SY5Y	NE\NRmlHfW6ldHnvckBie3OjeR?=		M{D4VlEhcHJ?	NE[ze4ZKdmirYnn0bY9vKG:oIGDLR{BqdiCqdX3hckBUUC2VWUXZJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiUF3BMZN1cW23bHH0[YQhVUGUQ1vTJJBpd3OyaH;yfYxifGmxbjDwdoVqdmO3YnH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nliUF3BMZN1cW23bHH0bY9vKG[xcjCxOUBucW6\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyxiSVO1NF0zOM7:TR?=	M3HJe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N\|c2Lz5{N{[0O\|M4PTxxYU6=
FADU	MlzZRY51cWOjbnPldkBie3OjeR?=		Mny2OFghcHK\|	MlPNRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF\BSHUh[2WubIOgcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OD13LkO5{txO	M4r3dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkexOFk4Lz5{N{[3NVQ6PzxxYU6=
DLD1	M4r0XmFvfGmlYX7j[ZIh[XO\|YYm=		M3rSdVQ5KGi{cx?=	NIjoOlFCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gSGxFOSClZXzsd{Bu\WG\|dYLl[EBi\nSncjC0PEBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUGxMlA3\|ryP	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ5MUS5O{c,Ojd4N{G0PVc9N2F-
A549	NGf2V2xCdnSrY3HuZ4VzKGG\|c3H5		M1:yR\|Q5KGi{cx?=	M4HScWFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJI1m[XO3cnXkJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO\|YYmsJGlEPTB;MUeuN:69VQ>?	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzZ5MUS5O{c,Ojd4N{G0PVc9N2F-
DU145	MWfBcpRq[2GwY3XyJIF{e2G7		MVO0PEBpenN?	NX[4fotjSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGRWOTR3IHPlcIx{KG2nYYP1doVlKGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NVgvODgQvF2=	NU\aNHc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2O\|E1QTdpPkK3OlcyPDl5PD;hQi=>
MCF7	NWi2[pIzSW62aXPhcoNmeiCjc4PhfS=>		NFPtc241QCCqcoO=	M{\3NmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDFVk1xd3OrdHn2[UBOS0Z5IHPlcIx{KG2nYYP1doVlKGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NVgvOTkQvF2=	MkHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4N{G0PVcoRjJ5NkexOFk4RC:jPh?=
MCF7	MUfDfZRwfG:6aXPpeJkh[XO\|YYm=		MV:xNlAhcHK\|	MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFEzOCCqcoOgZpkhVVSWIHHzd4F6NCCJSUWwQVYvQM7:TR?=	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF3MkSyPUc,OjhzNUK0Nlk9N2F-
T47D	NYnOc3V{S3m2b4TvfIlkcXS7IHHzd4F6		MVOxNlAhcHK\|	NXvKN4FXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXDR5RDDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygS2k2OD1zMD62{txO	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDF3MkSyPUc,OjhzNUK0Nlk9N2F-
SKBR3	MXzDfZRwfG:6aXPpeJkh[XO\|YYm=		MXixNlAhcHK\|	NEfYcGJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0KUMzDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiBzMkCgbJJ{KGK7IF3UWEBie3OjeTygS2k2OD1zMj61{txO	MkfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzNUK0NlkoRjJ6MUWyOFI6RC:jPh?=
MDA-MB-231	MVrDfZRwfG:6aXPpeJkh[XO\|YYm=		Mo\TNVIxKGi{cx?=	MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEGyNEBpenNiYomgUXRVKGG\|c3H5MEBIUTVyPUG1MlHPxE1?	M1vRclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUWyOFI6Lz5{OEG1NlQzQTxxYU6=
MCF7	NYXMdJptTnWwY4Tpc44h[XO\|YYm=		MWCyOEBpenN?	MoHRRY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCnc4Tyc4dmdi2rbnT1Z4VlKHS{YX7zZ5JqeHSrb36gdJJmcW6ldXLheIVlKG:4ZYLubYdpfCCob3zsc5dm\CCkeTDld5Rzd2enbjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OS54MEZOwG0>	NYG5b4ZTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyPVY{QThpPkK4Nlk3Ozl6PD;hQi=>
insect cells	NYjLcJFLTnWwY4Tpc44h[XO\|YYm=			NV3RXWFnTGm\|cHzhZ4Vu\W62IH;mJIZtfW:{ZYPj[YlvNWyjYnXs[YQh\XO2cnHkbY9tKCiobIXvdo9ud26nKTDmdo9uKGi3bXHuJJJm[2:vYnnuZY51KG[3bHytcIVv\3SqIIXueIFo\2WmIHXzeJJw\2WwIILlZ4VxfG:{IHLleIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{BjgSCobIXvdoV{[2WwY3WgdI9t[XKrenH0bY9vKGG\|c3H5MEBKSzVyPUCuNVg5\|ryP	NHzRZYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OESyOlk{OSd-Mki0NlY6OzF:L3G+
Ishikawa cells	MUXGeY5kfGmxbjDhd5NigQ>?		MV[3NkBpenN?	MYnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGW\|dILv[4VvKHKnY3XweI9zKGGucHjhM4JmfGFiaX6gbJVu[W5iSYPobYtif2FiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGG\|c3H5MEBKSzVyPUCuNljPxE1?	Ml74QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2Mk[5N\|EoRjJ6NEK2PVMyRC:jPh?=
MCF7	Mn\uRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MVq3NkBpenN?	M{DxZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgSXIu\GWyZX7k[Y51KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME20MlHPxE1?	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR{NkmzNUc,Ojh2Mk[5N\|E9N2F-
MCF7	NV\sV49FS3m2b4TvfIlkcXS7IHHzd4F6		NX;DeFYxPSCmYYnz	NH7VTYlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB3IHThfZMh[nliU2LCJIF{e2G7LDDJR\|UxRTRwNN88US=>	NVPNU\|dmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0NlY6OzFpPkK4OFI3QTNzPD;hQi=>
MCF7	Mn;jRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MmHDOFghcHK\|	NXvPOWF1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGWiCyb4PpeIl3\SCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVEvOzYQvF2=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4MEixPUc,Ojh2NkC4NVk9N2F-
Ishikawa cells	M2TKfWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M2q2fFQ5KGi{cx?=	NVLJepk5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE3NjR5zszN	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR4MEixPUc,Ojh2NkC4NVk9N2F-
MCF7	MnLwR5l1d3SxeHnjbZR6KGG\|c3H5		MYi0PEBpenN?	NETa[5FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDJR\|UxRTJzLkW3{txO	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDVyNUWzOkc,Ojh3MEW1N\|Y9N2F-
HeLa	Mof6R5l1d3SxeHnjbZR6KGG\|c3H5		Ml60NlQhcHK\|	MnTUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF05Njh3zszN	NEXobXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEizPFY6PCd-Mki4N\|g3QTR:L3G+
HeLa	MlrqSpVv[3Srb36gZZN{[Xl?		NH\lS4QzPCCqcoO=	NFr0eINCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IH\1cIwhdGWwZ4ToJGVT[WyyaHGgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO\|ZXSgbY4hcHWvYX6gTIVN[SClZXzsd{BqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDFVmUu\HKrdnXuJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjN2Md88US=>	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh6MkWwNkc,Ojh6OEK1NFI9N2F-
HeLa	M1fRPGZ2dmO2aX;uJIF{e2G7		M1fS[FI1KGi{cx?=	M33RXWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiZoXscEBt\W6pdHigSXJj\XSjIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJIh2dWGwIFjlUIEh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliRWLFMYRzcX[nbjDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUC9NU42O87:TR?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh6MkWwNkc,Ojh6OEK1NFI9N2F-
MCF7	NIXkbHBEgXSxdH;4bYNqfHliYYPzZZk>		M4mzUFczKGi{cx?=	MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDy[ZNignW{aX6gZoF{\WRiZnz1c5Jme2OnbnPlJIF{e2G7LDDJR\|UxRTdwOUVOwG0>	M4LsN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUK3O\|k2Lz5{OEmyO\|c6PTxxYU6=
insect cells	MoTDSpVv[3Srb36gZZN{[Xl?		NVnkWW1rOiCqcoO=	NFewXm5FcXOybHHj[Y1mdnRib3[g[ox2d3Knc3PlcpQh\XO2cn;n[Y4hdGmpYX7kJIZzd21icnXjc41jcW6jboSgbJVu[W5iRWLhcJBp[SCneIDy[ZN{\WRiaX6gbY5{\WO2IHPlcIx{KGmwY4XiZZRm\CCob4KgNkBpenNiYomgdI9t[XKrenH0bY9vNCCLQ{WwQVIvPjgQvF2=	NFLHSW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
MCF7	Mm\DR5l1d3SxeHnjbZR6KGG\|c3H5		NEnxdpo1QCCqcoO=	NHXrcmtEgXSxdH;4bYNqfHliaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zND6zOe69VQ>?	NX7KR4ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFIyOTNpPkK4PVQzOTF\|PD;hQi=>
Ishikawa cells	NXTFSWtLS3m2b4TvfIlkcXS7IHHzd4F6		MWC0PEBpenN?	MVrDfZRwfG:6aXPpeJkhcW5iaIXtZY4hUXOqaXvhe4Eh[2WubIOgZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBqdmO3YnH0[YQh\m:{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MkCuOlXPxE1?	NEjMV\|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEm0NlEyOyd-Mki5OFIyOTN:L3G+
MCF7	MW\GeY5kfGmxbjDhd5NigQ>?	MXOxNEBvVQ>?	M37u[\|I1KGi{cx?=	NV\LRnhxUW6qaXLpeIlwdiCxZjDFVoFteGijIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIEGwJI5OKGW\|dILv[4VvNWmwZIXj[YQheHKxZ3XzeIVzd26nIILlZ4VxfG:{IHX4dJJme3Orb36gZZQhOSC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2MkGxN{c,Ojh7NEKxNVM9N2F-
MCF7	MYrDfZRwfG:6aXPpeJkh[XO\|YYm=		M3u5TlI1KGi{cx?=	NXnVToNtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSC3cDD0c{AyODBidV2gZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeR?=	NEXKNVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC0OVE{PSd-MkmwOFUyOzV:L3G+
MCF7	MX3GeY5kfGmxbjDhd5NigQ>?	NWfOT2o{OSC3TR?=	NF;zZnA1KGi{cx?=	NIrSRWhKdmirYnn0bY9vKG:oIFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kAyP2KndHGt[ZN1emGmaX;sMY1m\GmjdHXkJJBUOiCvUl7BJIV5eHKnc4Ppc44h[XRiMTD1UUBxemWrbnP1ZoF1\WRiZn;yJFQhcHK\|IH\vcIxwf2WmIHL5JFE4[mW2YT3ld5Rz[WSrb3ygZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiMU[gbJJ{KGK7IIH1ZY51cXSjdHn2[UBTXC2SQ2KgZY5idHm\|aYO=	M2S5NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MES1NVM2Lz5{OUC0OVE{PTxxYU6=
U2O2	NVPoRZJ5TnWwY4Tpc44h[XO\|YYm=	MV2xJJVO	NU[4UZhbPCCqcoO=	NYKxRlE2UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDHSnAu\nW\|ZXSgSXJidHCqYTDMRmQhMDNyMTD0c{A2PTNicnXzbYR2\XNrIHX4dJJme3OnZDDpckBpfW2jbjDVNm8zKGOnbHzzJIF{e2W\|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKDF5YnX0ZU1me3S{YXTpc4wucW6mdXPl[EB1emGwc3PybZB1cW:wIHH0JFEhfU1icILlbY5kfWKjdHXkJIZweiB2IHjyd{Bnd2yub4fl[EBjgSCHMjDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{MDDodpMh[nl?	MnHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyNEWxN\|UoRjJ7MES1NVM2RC:jPh?=
MCF7	NYXIZlB6SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NUT1W4h3PDhiaILz	NWj2SmxVSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;OD6xNu69VQ>?	NGfzT5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC1OFM2QSd-MkmwOVQ{PTl:L3G+
MDA-MB-231	NIP3bWxCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M{WzRVQ5KGi{cx?=	NYPzfZpESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QS57Nt88US=>	NWLQNWhWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwOVQ{PTlpPkK5NFU1OzV7PD;hQi=>
HEK293	MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MW[0PEBpenN?	M333fGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTGVMOjl\|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlAvQTQQvF2=	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB3NEO1PUc,OjlyNUSzOVk9N2F-
MCF7	MkjDR4VtdCCleXPs[UBie3OjeR?=	NWP6PXFpOTBidV2=	M{fad\|I1KHSxIES4JIhzew>?	MXjD[YxtKGO7Y3zlJIFzemW\|dDDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KGGlY4XteYxifGmxbjDheEBIOC:JMTDwbIF{\SCjdDCxNEB2VSCjZoTldkAzPCC2bzC0PEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=>	NYPLb2hmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwOVQ{PTlpPkK5NFU1OzV7PD;hQi=>
MCF7	MWLDfZRwe3SjdHnjJIFkfGm4aYT5JIF{e2G7			Mlf5R5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOsJGlEPTB;Mj63{txO	NWXhfGxmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNFc{OzVpPkK5NlA4OzN3PD;hQi=>
MCF7	NGrJUGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?=		NWq0UlJ5PzJiaILz	MXXHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;NT6z{txO	MnnrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MkC3PFkoRjJ7MkKwO\|g6RC:jPh?=
MCF7	NH7uUo1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3PUS\|Q5KGi{cx?=	MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQS53zszN	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Mkm0OEc,Ojl2OEK5OFQ9N2F-
Ishikawa cells	NU\Yeo1vSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	MYO+OFAhfU1?	Mnu4OFghcHK\|	NXvGUFh6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDJd4hqc2G5YTDj[YxteyCjdDC+OFAhfU1iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigQ>?	M2fmTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEiyPVQ1Lz5{OUS4Nlk1PDxxYU6=
MCF7	NYHIVoVyTnWwY4Tpc44h[XO\|YYm=	NHXMOJQyKHWP		MnXJRY51[WexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGW\|dILh[IlwdC2rbnT1Z4VlKFCpUjDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCkeTDTXWJTKEe{ZXXuJIR6\SCkYYPl[EBTXC2SQ2KgZY5idHm\|aYO=	NHvaTms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS4Nlk1PCd-Mkm0PFI6PDR:L3G+
MCF7	M2nTdWZ2dmO2aX;uJIF{e2G7		NYDZSIs3OjRiaILz	NFzJTGFKdmS3Y4Tpc44hd2Zic3Xs[YN1cX[nIHXzeJJw\2WwIILlZ4VxfG:{IHHsdIhiKGSnZ4Lh[IF1cW:wIHnuJIh2dWGwIF3DSlch[2WubIOgbIFz[m:{aX7nJHRMNUWURT3MeYMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIV{fHKjZHnvcE1qdmS3Y3XkJJRz[W6\|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5MEBKSzVyPUGuOlA2\|ryP	NHywUnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2Nlc{Pyd-Mkm1OlI4Ozd:L3G+
MCF7	MoD5SpVv[3Srb36gZZN{[Xl?	MUi2NEBu\y:tZx?=	M{nWRlQ5KGSjeYO=	MVXEc5dvemWpdXzheIlwdiCxZjDwdo9o\XO2ZYLvcoUhemWlZYD0c5IhdVKQQTDs[ZZmdCCrbjDOU2QhW0OLRDDnZY1u[SCvb4Xz[UB5\W6xZ4Lh[pRm\CC5aYToJIh2dWGwIF3DSlch[2WubIOgZZQhPjBibXevb4ctKHCxIEWg[IF6eyCyZYKge4VmcyCob4KgOFgh\GG7czDwc5N1KHS3bX;yJIlueGyjboTheIlwdiCvZXHzeZJm\CB5IHjyd{Bxd3O2IHzhd5Qh\G:\|ZTDifUBTXC2SQ2KgcYV1cG:m	NHXLS5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW2Nlc{Pyd-Mkm1OlI4Ozd:L3G+
insect cells	MUnGeY5kfGmxbjDhd5NigQ>?		M1T5XlIhcHK\|	NXjNNIp2TGm\|cHzhZ4Vu\W62IH;mJGZtfW:{bX;u[UBGWzJiR4Ll[Y4h\nKxbTDmeYxtKGynbnf0bEBpfW2jbjDFVk1idHCqYTDlfJBz\XO\|ZXSgbY4hcW6\|ZXP0JINmdGy\|IHHmeIVzKDJiaILzJIJ6KG[udX;y[ZNk\W6lZTDwc4xiemm8YYTpc44h[XO\|YYmsJGlEPTB;MT60Nu69VQ>?	M2DGfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi3NlIyLz5{OUW4O\|IzOTxxYU6=
MCF7	NVvtZ4k6SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		MmP5OFghcHK\|	NHK2dlJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zNT60Ou69VQ>?	M{\kb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi3NlIyLz5{OUW4O\|IzOTxxYU6=
Ishikawa cells	MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MV:0PEBpenN?	MkjERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCLc3jpb4F4[SClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUK2MlUz\|ryP	NFTCb409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4O\|IzOSd-Mkm1PFczOjF:L3G+
MRC5 SV2	NHTSWoNEgXSxdH;4bYNqfHliYYPzZZk>		MVS3NkBpenN?	MoHFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUXJEPSCVVkKgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KHKnc3H6eZJqdiCmeXWtZoF{\WRiZnz1c5JqdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUGwMlLPxE1?	M2X5XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkC0OVQyLz5{OU[wOFU1OTxxYU6=
MCF7	NH:0VINEgXSxdH;4bYNqfHliYYPzZZk>		M1zzT\|QhcHK\|	M2ficGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMheHKnaX7jeYJifGWmIH\vdkA1KGi{czDmc4xtd3enZDDifUBqdmO3YnH0bY9vKGmwIHPvcZBwfW6mIH\y[YUhdWWmaXGg[o9zKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QC54Md88US=>	M3XXXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NkC1PFA5Lz5{OU[wOVgxQDxxYU6=
DLD1	MUXDfZRwfG:6aXPpeJkh[XO\|YYm=		M2DYRlQhcHK\|	NHLzXJZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFVERzIHPlcIx{KHC{ZXnuZ5Vj[XSnZDDmc5IhPCCqcoOg[o9tdG:5ZXSgZpkhcW6ldXLheIlwdiCrbjDjc41xd3WwZDDmdoVmKG2nZHnhJIZweiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTF4LkROwG0>	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZyNUiwPEc,Ojl4MEW4NFg9N2F-
A549	NFj6bXpEgXSxdH;4bYNqfHliYYPzZZk>		MWO0JIhzew>?	MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJJBz\WmwY4XiZZRm\CCob4KgOEBpenNiZn;scI94\WRiYomgbY5kfWKjdHnvckBqdiClb33wc5Vv\CCocnXlJI1m\GmjIH\vdkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE4NjQQvF2=	Mo\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4MEW4NFgoRjJ7NkC1PFA5RC:jPh?=
HeLa	MYXGeY5kfGmxbjDhd5NigQ>?		NV3ZPXVtPC53IHjydy=>	NXHuTWFDUW6qaXLpeIlwdiCxZjDFZo9t[X[rcoXzJIdtgWOxcILveIVqdi:vYYTybZgheHKxdHXpckBXWDRyIHXueJJ6KGmwIHj1cYFvKEinTHGgZ4VtdHNiYX\0[ZIhPC53IHjyd{Bj\XSjLXzhZ5RidWG\|ZTDy[ZBwenSncjDhd5NigSxiSVO1NF0xNjd\|zszN	MoC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl4MkSzPFcoRjJ7NkK0N\|g4RC:jPh?=
MRC5	M3XWdGN6fG:2b4jpZ4l1gSCjc4PhfS=>		M33a[VczKGi{cx?=	MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNVmM2KGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBz\XOjeoXybY4h[XO\|YYmsJGlEPTB;MUFOwG0>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTZ5MkC0NUc,Ojl4N{KwOFE9N2F-
MCF7	M{PoVWFvfGm2dX3vdkBie3OjeR?=	MVW2NEBu\y:tZx?=	NFfpUJA1KHenZXvz	MWjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IIjlco9oemGodHXkJIlvKG63L371JI1wfXOnIH3v[IVtKG:oIIThcY95cW[nbj3z[Y5{cXSrdnWgZpJm[XO2IHPhcoNmeiC6ZX7v[5Ji\nRiaX3wcIFvfGWmIIfpeIghOTekZYThMYV{fHKjZHnvcEB1cW2nIILlcIVie2VicHXscIV1eyCjc4Pld5Nm\CCjczDy[YR2[3Srb36geJVud3Jidn;seY1mKGG2IE[wJI1oN2upLDDwc{By\CCob4KgOEB4\WWtcx?=	MojmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByOE[2NlYoRjNyMEi2OlI3RC:jPh?=
insect	M{j4[GZ2dmO2aX;uJIF{e2G7			MUfCbY5lcW6pIHHm[olvcXS7IITvJJVvfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JIZ2dGxibHXu[5RpKEWUYXzwbIEh\XiycnXzd4VlKGmwIHnud4VkfCClZXzsd{AxNjFidH:gNVAxODBidV2gZpkhTmy3b4Ltc45mKEWVMjDHdoVmdiCmaYPwcIFk\W2nboSgZoF{\WRiYYPzZZk>	NIjHVmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEC5NVkzOCd-M{CwPVE6OjB:L3G+
HepG2	Mn7rR5l1d3SxeHnjbZR6KGG\|c3H5		MX6yOEBpenN?	MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjCyOEBpenNiYomgdoV{[Xq3cnnuJIR6\S2kYYPl[EBndHWxcnXzZ4Vv[2ViYX7hcJl{cXNuIFPDOVA:OjRwN988US=>	M{PMd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{KyO\|U1Lz5\|MEOyNlc2PDxxYU6=
HEK293	NWj5[Jl[S3m2b4TvfIlkcXS7IHHzd4F6		NY[3ZVExOjRiaILz	MkHHR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy\|IHHmeIVzKDJ2IHjyd{BjgSC{ZYPhfpVzcW5iZInlMYJie2WmIH\seY9z\XOlZX7j[UBidmGueYPpd{whS0N3ME2zPU41\|ryP	NFTK[\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEOyNlc2PCd-M{CzNlI4PTR:L3G+
MCF7	Mn2yRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		MofENlQhcHK\|	NE\wV\|VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD15LkC5{txO	NWHSOJNxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlA6PTJpPkOwN\|YxQTV{PD;hQi=>
MDA-MB-231	NGTsVndCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		MmTCNlQhcHK\|	Mm[xRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFcvOc7:TR?=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4MEm1Nkc,OzB\|NkC5OVI9N2F-
insect cells	NVzYdYVKTnWwY4Tpc44h[XO\|YYm=		MlTmNkBpenN?	NXTnWlE2TGm\|cHzhZ4Vu\W62IH;mJIZtfW:{ZYPj[Y51KGW\|dILv[4VvKGyrZ3Hu[EBnem:vIILlZ49u[mmwYX70JIh2dWGwIH\1cIwhdGWwZ4ToJJVvfGGpZ3XkJGVT[WyyaHGg[ZhxemW\|c3XkJIlvKFOyb3TvdJRmemFiZoL1[4lx\XKmYTDpcpNm[3RiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigSxiSVO1NF0yNjV4zszN	NFLlR5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEO4OFA1Pyd-M{CzPFQxPDd:L3G+
MCF7	MWfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MmTPOFghcHK\|	NX60bZFJSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MU[uO:69VQ>?	MnrlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB\|OESwOFcoRjNyM{i0NFQ4RC:jPh?=
MCF7	MVfGeY5kfGmxbjDhd5NigQ>?	NIW1[mUyKHWP		NYnicpMzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIFWyMYlv\HWlZXSgdJJw\2W\|dHXyc45mKHKnY3XweI9zKG2UTlGg[ZhxemW\|c3nvckBifCBzIIXNJIJ6KFO\QmKg[5Jm\W5iZInlJIJie2WmIGLUMXBEWiCvZYToc4Q>	NH[4b5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEO4OFA1Pyd-M{CzPFQxPDd:L3G+
MDA-MB-231	M33xTmZ2dmO2aX;uJIF{e2G7			NUPZS2RRSmmwZHnu[{Bi\m[rbnn0fUB1dyC{Ul7BMWEhe2m2ZTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZicILveIVqdiC2cnHud4xifGmxbjygTWM2OD1zOD6wPO69VQ>?	MnL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB3M{CxO\|QoRjNyNUOwNVc1RC:jPh?=
Lucena 1	MlzISpVv[3Srb36gZZN{[Xl?	M1LvVlEzNjVidV2=	NE\lUngyKGi{	M{P3U2lvcGmkaYTpc44hd2ZiUD3ndE1u\WSrYYTl[EBTcG9vMUKzJIVn\my3eDDpckBpfW2jbjDMeYNmdmFiMTDj[YxteyCjdDCxNk42KHWPIIDy[Ylv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCUaH:tNVI{KGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFEhcHJiYomg[oxwfyCleYTvcYV1enl?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyOEc,OzB4MUOzNlQ9N2F-
Lucena 1	NH3sRZJHfW6ldHnvckBie3OjeR?=	M4D4[FI2KHWP	MYOxJIhz	NEm1flZKdmirYnn0bY9vKG:oIGCt[5AudWWmaXH0[YQhWmixLUGyN{Bm\m[udYigbY4hcHWvYX6gUJVk\W6jIEGgZ4VtdHNiYYSgNlUhfU1icILlbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KFKqbz2xNlMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNUBpeiCkeTDmcI94KGO7dH;t[ZRzgQ>?	MojKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4MUOzNlQoRjNyNkGzN\|I1RC:jPh?=
HepG2	MYXDfZRwfG:6aXPpeJkh[XO\|YYm=		M{D3bVI1KGi{cx?=	M2roT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVLPxE1?	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZzM{OyO{c,OzB4MUOzNlc9N2F-
MRC5 SV2	MXjDfZRwfG:6aXPpeJkh[XO\|YYm=		NF\zfWE{KGSjeYO=	NGm3TIpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOWkN3IGPWNkBk\WyuczDh[pRmeiB\|IHThfZMh[nlicnXzZZp2emmwIHT5[UBj[XOnZDDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTB;MUGuNlPPxE1?	MmLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB4M{i3OlEoRjNyNkO4O\|YyRC:jPh?=
MRC5	NWjWO\|RxS3m2b4TvfIlkcXS7IHHzd4F6		M4H3fFI1KGi{cx?=	M1u2V2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1TSzViY3XscJMh[W[2ZYKgNlQhcHK\|IHL5JJJme2G8dYLpckBlgWViYnHz[YQh[XO\|YYmsJGlEPTB;MUGuNFnPxE1?	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODZ7MkCyOEc,OzB4OUKwNlQ9N2F-
T47D	MnvTRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NGXaWGMzPCCqcoO=	M1j6e2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVES3SEBk\WyuczDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTN2LkSy{txO	NU\OR2dORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C3N\|E{QTdpPkOwO\|MyOzl5PD;hQi=>
MCF7	M{jXemFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M3XvWFI1KGi{cx?=	NYSzeIpiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;NEKuOO69VQ>?	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODd\|MUO5O{c,OzB5M{GzPVc9N2F-
MRC5	MWrDfZRwfG:6aXPpeJkh[XO\|YYm=		MVuyOEBpenN?	NU\V[Gh7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVKFNTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IILld4F7fXKrbjDkfYUh[mG\|ZXSg[ox2d3KrbXX0dolkKGG\|c3H5MEBESzVyPUGxMlA6\|ryP	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODh3N{e0PUc,OzB6NUe3OFk9N2F-
MRC5	M4HCRWN6fG:2b4jpZ4l1gSCjc4PhfS=>		MmTFNlQhcHK\|	M4e5NGN6fG:2b4jpZ4l1gSCrbjDoeY1idiCPUlO1JINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDy[ZNignW{aX6tZoF{\WRiY4n0c5RwgGmlaYT5JIF{e2G7LDDJR\|UxRTFzLkC5{txO	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODh5OUizPUc,OzB6N{m4N\|k9N2F-
HEK293	NWfW[llCS3m2b4TvfIlkcXS7IHHzd4F6		M3zJOlIxKGi{cx?=	MkLnR5l1d3SxeHnjbZR6KGGpYXnud5QhUEWNMkmzJINmdGy\|IH3lZZN2emWmIHHmeIVzKDJyIHjyd{BjgSC{ZYPhfpVzcW5iZInlJIJie2WmIH\seY9z\XOlZX7j[UBie3OjeTygTWM2OD1{NN88US=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODl5NUWwNkc,OzB7N{W1NFI9N2F-
A2058	NGPreIdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NF;Ke2wzPCCqcoO=	MnjwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDMkC1PEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK\|IHL5JINmdGy2aYTldk1jdHWnIHHzd4F6NCCHQ{WwQVEzNjYQvF2=	NGT6fnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUC5PVU3QCd-M{GwPVk2Pjh:L3G+
MelJuSo	MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M1vsSlI1KGi{cx?=	NX65UG5sSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDN[YxLfVOxIHPlcIx{KGG\|c3Xzd4VlKGG\|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliY3XscJRqfGW{LXLseYUh[XO\|YYmsJGVEPTB;MUSuOu69VQ>?	M1HTNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMEm5OVY5Lz5\|MUC5PVU3QDxxYU6=
MCF7	M3jnTWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NILNbJM1QCCqcoO=	MofjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyCFZXzsd{Bi\nSncjC0PEBpenNiYomgcoV2fHKjbDDy[YQh\HmnLXLhd4VlKGG\|c3H5MEBKSzVyPUVOwG0>	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTF{OUS1OUc,OzFzMkm0OVU9N2F-
SiHa	MkPDR5l1d3SxeHnjbZR6KGG\|c3H5			M3PJc2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNqUGFiY3XscJMtKEeLNUC9NE4yOs7:TR?=	NIDUS\|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUKwNFI{Pid-M{GyNFAzOzZ:L3G+
PANC1	Mmi4R5l1d3SxeHnjbZR6KGG\|c3H5			MmTjR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yPc7:TR?=	M2PKPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMkCwNlM3Lz5\|MUKwNFI{PjxxYU6=
MDA-MB-231	M3HUNWN6fG:2b4jpZ4l1gSCjc4PhfS=>			MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|LDDHTVUxRTBwMkVOwG0>	Mnm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF{MECyN\|YoRjNzMkCwNlM3RC:jPh?=
J774A.1	MXfDfZRwfG:6aXPpeJkh[XO\|YYm=		NV3BSGRwOjRiaILz	NFXYd4VEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBLPzd2QT6xJINmdGy\|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDu[ZV1emGuIILl[EB2eHSja3WgZZN{[XluIFPDOVA:OTFwMEdOwG0>	Mmr0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF\|N{[1OlkoRjNzM{e2OVY6RC:jPh?=
MCF7	NWi5U3FmS3m2b4TvfIlkcXS7IHHzd4F6	MlrGNE4yKHSxIEGwNEB2VQ>?	NGT4fYczPCCqcoO=	MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjdDCwMlEhfG9iMUCwJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK\|IHL5JG1VXCCjc4PhfS=>	NIHHXJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUWxOFA3OCd-M{G1NVQxPjB:L3G+
MCF7	MlPhRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			M2nMVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2=	M2W0ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUS2NVk4Lz5\|MUW0OlE6PzxxYU6=
A549	MWXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			M2HC[WFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2=	NV\2W4hrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OFYyQTdpPkOxOVQ3OTl5PD;hQi=>
HeLa	NHPQ[ohCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=			MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEinTHGgZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyPUGwMlg4\|ryP	NGjCVoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUW0OlE6Pyd-M{G1OFYyQTd:L3G+
DU145	MWLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			MnTHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCGVUG0OUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2=	NGKwWVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUW0OlE6Pyd-M{G1OFYyQTd:L3G+
MDA-MB-231	MoXZRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			M3myNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ\|MTDj[YxteyCkeTDNWHQh[XO\|YYmsJGlEPTB;MUGuNu69VQ>?	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV2NkG5O{c,OzF3NE[xPVc9N2F-
SiHa	NHHHfJpIem:5dHigbY5pcWKrdHnvckBie3OjeR?=			NIHwSmRIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBUcUijIHPlcIx{NCCJSUWwQVAvOTMQvF2=	MnTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MEW4OlUoRjNzNkC1PFY2RC:jPh?=
MDA-MB-231	M{jWSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7			M4O5Zmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj2yN\|Eh[2WubIOsJGdKPTB;MD6xNu69VQ>?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZyNUi2OUc,OzF4MEW4OlU9N2F-
PANC1	NYfYeWtmT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=			MmG3S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yOs7:TR?=	NGi4THE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MU[wOVg3PSd-M{G2NFU5PjV:L3G+
MCF7	NV7oTYUySW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=			M1jjWmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVI2\|ryP	MoLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MUCzO\|goRjNzNkGwN\|c5RC:jPh?=
M-HeLa	NEjuS5ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=			MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE1vSHXMZUBk\WyuczDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVI5\|ryP	NHvJVY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MU[xNFM4QCd-M{G2NVA{Pzh:L3G+
Chang	M160XGN6fG:2b4jpZ4l1gSCjc4PhfS=>			MXfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDbIFv\yClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYomg[ox2d3Knc3PlcoNmKGKjc3XkJJZq[WKuZTDj[YxtKGOxdX70bY5oKG2ndHjv[EwhUUN3ME20Nk4y\|ryP	Mnm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF4MUCzO\|goRjNzNkGwN\|c5RC:jPh?=
MCF7	MnXHR5l1d3SxeHnjbZR6KGG\|c3H5		NUTJcXh7PDhiaILz	M13KPWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zOi54zszN	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZ{MEKyOUc,OzF4MkCyNlU9N2F-
RAW264.7	MWnDfZRwfG:6aXPpeJkh[XO\|YYm=		NETHT40zPCCqcoO=	NYSzbYN5S3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBv\XW2cnHsJJJm\CCmeXWgZoF{\WRiYYPzZZktKEOFNUC9NVEvOc7:TR?=	M3\0[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzN{CzPFE5Lz5\|MUewN\|gyQDxxYU6=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-AKT / AKT ; PubMed: 30759864 Cancers (Basel) MCF-7 cells were treated with 2 μM tamoxifen and harvested at the indicated time point to analyze the expressions of p-AKT and AKT by western blot. ER-α66 / ER-α36 / HER2 / EGFR ; PubMed: 25203051 PLoS One Western blot analysis of the expression levels of ER-α66, ER-α36, HER2 and EGFR in ER-positive breast cancer MCF7 (A), T47D (B) and H3396 (C) cells treated with 1 µM of tamoxifen (TAM) for indicated time period. p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; PubMed: 27020861 Cancer Res B. Immunoblot of AMPK pathway activation following treatment with 30μM tamoxifen in MCF7 and C. in MDA-MB-231 cells. CD36 / p-ERK / ERK ; PubMed: 30573731 Oncogenesis MCF-7 cells MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h, or with 5 μM Tam for the indicated times. Expression of CD36, p-ERK1/2 and ERK1/2 was determined by western blot.	30759864 25203051 27020861 30573731
Immunofluorescence	CD36; PubMed: 30573731 Oncogenesis MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h. Expression of CD36 was determined by immunofluorescent staining .	30573731

In vivo

Tamoxifen administration to rapidly growing, estradiol-stimulated MCF-7 xenografts results in a dose-dependent retardation or cessation of tumor growth by significantly decreasing tumor cell proliferation in tumor. Tamoxifen treatment results in a slowing of tumor growth (tumor doubling time, 12 days), a significant increase in tumor potential doubling time (T_pot) (6.6 days), and a decrease in labeling index (%LI) (to 8%) by 23 days posttreatment, compared with untreated mice which shows a volume doubling time of 5 days, a T_pot of 2.3 days, and a %LI of 23%. ^[5] Tamoxifen has not only antiestrogenic but also estrogenic properties depending on the species, tissue, and gene. Tamoxifen displays favorable effects on bone and serum lipid concentrations and stimulation endometrium. ^[1]

Protocol

Kinase Assay: ^[3]	- Collapse Competitive binding assays: Cells are harvested from 150-sq cm T-flasks, and cytosol is prepared at a protein concentration of approximately 2 mg/mL in phosphate buffer. Aliquots of this 180,000 ×g supernatant are then incubated with various concentrations of Tamoxifen and 2.5 nM [³H]estradiol for 16 hr at 0-4 ℃. The free steroids are absorbed by dextran-charcoal [l0 μL of 0.5% Dextran C-5% Norite A in TE buffer] for 1 hr at 0 ℃, and aliquots are counted after centrifugation at 800 ×g, 30 min. The relative binding ability of each competitor is taken as the ratio of the concentration of radioinert estradiol/competitor required to inhibit one-half of the specific [³H]estradiol binding, with the affinity of estradiol set at 100%.
Cell Research: ^[3]	- Collapse Cell lines: Human breast cancer cells MCF-7 Concentrations: ~1 μM Incubation Time: 10 days Method: MCF-7 cells are seeded into T-25 flasks (1.5×10⁵ cells/flask) and grown for 2 days in the MEM supplemented with 10 mM HEPES buffer, gentamicin (50 μg/mL), penicillin (100 units/mL), streptomycin (0.1 mg/mL), bovine insulin (6 ng/mL), hydrocortisone (3.75 ng/mL), and 5% calf serum that has been treated with dextran-coated charcoal for 45 min at 55 ℃ to remove endogenous hormones. The medium is then changed to MEM supplemented as described above, except that it contains 2% charcoal dextran-treated calf serum and various concentrations of Tamoxifen. At the end of incubation, cell numbers are counted. (Only for Reference)
Animal Research: ^[5]	- Collapse Animal Models: Human breast carcinoma xenografts MCF-7 Dosages: 2 cm Tamoxifen capsules Administration: Subcutaneous implantation (Only for Reference)

References

- Collapse

Solubility (25°C)

In vitro	DMSO	100 mg/mL (177.41 mM)
	Water	Insoluble
	Ethanol	''''100 mg/mL
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 30% PEG400+0.5% Tween80+5% propylene glycol For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Tamoxifen (ICI 46474) Citrate SDF

Molecular Weight	563.64
Formula	C₂₆H₂₉NO.C₆H₈O₇
CAS No.	54965-24-1
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CCC(=C(C1=CC=CC=C1)C2=CC=C(C=C2)OCCN(C)C)C3=CC=CC=C3.C(C(=O)O)C(CC(=O)O)(C(=O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04200066	Not yet recruiting	Drug: Temozolomide Tamoxifen Maprotiline	Glioblastoma\|Brain Tumor	University of Rochester	May 1 2021	Phase 1
NCT04433494	Not yet recruiting	Drug: TY-302: capsule 25mg/50mg ; Tamoxifen: tablet10mg	Breast Cancer\|Solid Tumor	TYK Medicines Inc	October 2020	Phase 1
NCT04174352	Recruiting	Drug: Tamoxifen\|Diagnostic Test: FES PET/CT	ERα+ Breast Cancer\|ESR1 Gene Mutation	University of Wisconsin Madison	October 20 2020	Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?
Answer:
The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

Estrogen/progestogen Receptor Signaling Pathway Map

Estrogen/progestogen Receptor Inhibitors with Unique Features

Selective AR agonist

Andarine : Selective non-steroidal AR agonist, K_i=4 nM.
Most Potent AR Antagonist

ARN-509 : Androgen receptor, IC50=16 nM.
FDA-approved AR Antagonist

Bicalutamide : Approved by FDA for prostate cancer.
Newest AR Antagonist

AZD3514 : Potent and oral androgen receptor downregulator with K_i of 2.2 μM.

Related Estrogen/progestogen Receptor Products

Licochalcone A ^New

Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.
Endoxifen HCl ^New

Endoxifen HCl, the active metabolite of Tamoxifen, ia a potent and selective estrogen receptor antagonist. Phase 2.
Sacubitril/valsartan (LCZ696) ^New

Sacubitril/valsartan (LCZ696, Sacubitril, Valsartan), consisting of valsartan and sacubitril in 1:1 molar ratio, is an orally bioavailable, dual-acting angiotensin receptor-neprilysin inhibitor (ARNi) for hypertension and heart failure. Phase 3.
Fulvestrant (ICI-182780)

Fulvestrant (ICI-182780, ZD 9238, ZM 182780) is an estrogen receptor (ER) antagonist with IC50 of 0.94 nM in a cell-free assay. Fulvestrant also induces autophagy and apoptosis and has antitumor activity.
Mifepristone (RU486)

Mifepristone (RU486, C-1073, RU 38486, Mifegyne) is a remarkably active antagonist of progesterone receptor and glucocorticoid receptor with IC50 of 0.2 nM and 2.6 nM, respectively. Mifepristone promotes cell autophagy and apoptosis, decreases Bcl-2 level and increases Beclin1 level, accompanied by weakened interaction between Bcl-2 and Beclin1.

Features:Mifepristone is the first approved medication for patients with endogenous cushing's syndrome.
Raloxifene HCl

Raloxifene (LY156758, Keoxifene) HCl a selective and orally active estrogen receptor modulator (SERM), which inhibits human cytosolic aldehyde oxidase-catalyzed phthalazine oxidation activity with IC50 of 5.7 nM.

Features:Raloxifene is as effective as tamoxifen in reducing the incidence of breast cancer in postmenopausal women at increased risk.
Estradiol

Estradiol (17β-estradiol, β-Estradiol, E2, 17β-Oestradiol) is a human sex hormone and steroid, and the primary female sex hormone. Estradiol upregulates IL-6 expression through the estrogen receptor β (ERβ) pathway.
Toremifene Citrate (NK 622)

Toremifene Citrate (NK 622, NSC 613680) is an oral selective estrogen receptor modulator (SERM), used in the treatment of advanced breast cancer.
Progesterone (NSC 9704)

Progesterone (NSC 9704, NSC 64377, Pregn-4-ene-3,20-dione) is an endogenous steroid hormone involved in the menstrual cycle, pregnancy, and embryogenesis of humans and other species. A potent agonist of the nuclear progesterone receptor (nPR) with Kd of 1 nM; An agonist of the membrane progesterone receptors(mPRs); An antagonist of the σ1 receptor.
Bazedoxifene (WAY-140424) HCl

Bazedoxifene HCl (WAY-140424, TSE-424) is a novel, non-steroidal, indole-based estrogen receptor modulator (SERM) binding to both ERα and ERβ with IC50 of 23 nM and 89 nM, respectively.

Choose Your Country or Region

By Signaling Pathways

By product type

Tamoxifen (ICI 46474) Citrate