Equol

For research use only.

Catalog No.S2450

1 publication

Equol Chemical Structure

CAS No. 531-95-3

Equol ,a metabolite of soybeans, is an important isoflavone in humans,specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.

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10mM (1mL in DMSO) USD 70 In stock
USD 79 In stock
USD 170 In stock
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Selleck's Equol has been cited by 1 publication

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Description Equol ,a metabolite of soybeans, is an important isoflavone in humans,specifically binds to 5α-DHT, has a modest affinity for recombinant estrogen receptor ERβ. Phase 2.
Features A chiral molecule that can exist as the enantiomers R-equol and S-equol.
Targets
ERβ [1]
In vitro

Equol is a metabolite produced from the soy phytoestrogen daidzein by the action of gut microflora. Equol has higher affinity for both ERs (estrogen receptors including ERalpha and ERbeta). Equol exists as the enantiomers R-equol and S-equol. S-equol has a high binding affinity, preferential for ERbeta with a Ki of 16 nM whereas R-equol binds more weakly and with a preference for ERalpha K with a Ki of 50 nM. [1] Equol is superior to all other isoflavones in its antioxidant activity. [2] Equol has antioestrogenic properties. [3] Equol is a 100-fold more potent than daidzein in stimulating an oestrogenic response. Equol is also more effective than daidzein in competing with 3 H-oestradiol for binding to the ER. Equol stimulates the growth of MCF-7 cells in a concentration-dependent manner. Although equol exhibits oestrogenic activity, exposure of MCF-7 cells to equol simultaneously with oestradiol is effective in reducing pS2 mRNA expression. Equol results in the downregulation of ER mRNA expression. [4]

Protocol

Cell Research:[4]
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  • Cell lines: MCF-7 cells
  • Concentrations: 0 μM-10 μM
  • Incubation Time: 24 hours
  • Method: Each well of a 24-well plate is seeded with 1 × 105 MCF-7 cells in 1 mL of media B. Twenty-four hours after plating, equol at the indicated concentration is added. Equol is dissolved in ethanol (final concentration of ethanol in the medium is 1%). The medium is changed every 24 hours and equol is replenished with each change. Cell growth is determined on the sixth day by the sulphorhodamine colorimetric assay. After colour development, aliquots are pipetted into a 96-well microtitre plate and the absorbance is determined at 570 nm using an Elisa microplate reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 48 mg/mL (198.12 mM)
Ethanol 48 mg/mL (198.12 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 242.27
Formula

C15H14O3

CAS No. 531-95-3
Storage powder
in solvent
Synonyms N/A
Smiles C1C(COC2=C1C=CC(=C2)O)C3=CC=C(C=C3)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01982578 Recruiting Dietary Supplement: Genistein|Other: Placebo Alzheimer''s Disease Fundación para la Investigación del Hospital Clínico de Valencia|University of Valencia September 1 2017 Not Applicable
NCT03101085 Recruiting Drug: S-equol|Other: Placebo Alzheimer Disease Russell Swerdlow MD|Ausio Pharmaceuticals LLC|University of Kansas Medical Center May 5 2017 Phase 1|Phase 2
NCT02142777 Completed Drug: S -Equol|Drug: Placebo Alzheimer''s Disease Russell Swerdlow MD|Ausio Pharmaceuticals LLC|University of Kansas Medical Center July 2014 Phase 1
NCT00998920 Completed Drug: S-equol|Drug: Placebo Healthy Ausio Pharmaceuticals LLC December 2008 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID