Licochalcone A

Catalog No.S7828

For research use only.

Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.

Licochalcone A Chemical Structure

CAS No. 58749-22-7

Selleck's Licochalcone A has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description Licochalcone A is an estrogenic flavanoid extracted from licorice root, showing antimalarial, anticancer, antibacterial and antiviral activities. Phase 3.
In vitro

Licochalcone A markedly inhibits the in vitro growth of L. major amastigotes in human MDMs and U937 cells. [1] Licochalcone A shows antibacterial effects against all gram-positive bacteria tested and especially against all Bacillus spp. Tested with MICs of 2 to 3 micrograms/mL. [3] In CT-26 colon cancer cells, Licochalcone A reduces the cell viability and DNA synthesis. [4] Licochalcone A also interferes with MAPK signaling cascades, initiates ROS generation, induces oxidative stress and consequently causes BGC cell apoptosis. [5]

In vivo In mice infected with L. major, licochalcone A (5 mg/kg, i.p.) completely prevents lesion development. In mice infected with L. donovani, licochalcone A (150 mg/kg, p.o.) results in > 65 and 85% reductions of parasite loads in the liver and the spleen, respectively. [2] In CT-26 cell-inoculated Balb/c mice, licochalcone A (1 mg/kg, p.o.) inhibits the tumor growth, and alleviates cisplatin-induced nephrotoxicity and hepatotoxicity. [4]

Protocol (from reference)

Cell Research: [4]
  • Cell lines: CT-26 cells
  • Concentrations: ~20 μM
  • Incubation Time: 72 hours
  • Method: The viability of CT-26 mouse colon cancer cells is determined via a MTT assay. In brief, colon cancer cells are seeded onto each well of a 96-well plate with DMEM containing 10% FBS and cultured to adhere overnight. The cells are then treated with various concentrations of LCA in serum-free medium for 24 and 72 hr, respectively. Twenty microlitres of a MTT solution (5 mg/ml) is added to each well, and the cells are incubated for 4 hr at 37°. The medium is then removed, and 200 µL of dimethyl sulfoxide is added to each well. The absorbance is determined at 570 nm using a microplate reader.
Animal Research:[2]
  • Animal Models: Mice infected with L. major
  • Dosages: 5 mg/kg/day
  • Administration: i.p. or p.o.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 338.4
Formula

C21H22O4

CAS No. 58749-22-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)(C=C)C1=C(C=C(C(=C1)C=CC(=O)C2=CC=C(C=C2)O)OC)O

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04002024 Completed Other: Moisturizer with active ingredients|Other: Placebo Acne Vulgaris|Acne Mahidol University|Beiersdorf June 1 2019 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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