For research use only.
CAS No. 57-88-5
Cholesterol is the principal sterol of all higher animals required to build and maintain membranes.
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|Description||Cholesterol is the principal sterol of all higher animals required to build and maintain membranes.|
Cholesterol serves as a precursor for the biosynthesis of steroid hormones, bile acids, and vitamin D. It modulates membrane fluidity over the range of physiological temperatures. The hydroxyl group on cholesterol interacts with the polar head groups of the membrane phospholipids and sphingolipids, while the bulky steroid and the hydrocarbon chain are embedded in the membrane, alongside the nonpolar fatty-acid chain of the other lipids. Through the interaction with the phospholipid fatty-acid chains, cholesterol increases membrane packing, which reduces membrane fluidity. The structure of the tetracyclic ring of cholesterol contributes to the decreased fluidity of the cell membrane as the molecule is in a trans conformation making all but the side chain of cholesterol rigid and planar. In this structural role, cholesterol reduces the permeability of the plasma membrane to neutral solutes, protons, and sodium ions.
|In vivo||Cholesterol is recycled. The liver excretes it in a non-esterified form (via bile) into the digestive tract. Typically about 50% of the excreted cholesterol is reabsorbed by the small bowel back into the bloodstream.|
|In vitro||DMSO||0.03 mg/mL (0.07 mM)|
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|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
|Dosage||mg/kg||Average weight of animals||g||Dosing volume per animal||ul||Number of animals|
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|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04616053||Not yet recruiting||Behavioral: IMPACT intervention||Diabetes Mellitus Type 2||Pole de Sante Chambery Nord||September 2021||Not Applicable|
|NCT04298125||Not yet recruiting||Behavioral: Exercise in Pregnancy||Pregnancy Related||Arkansas Children''s Hospital Research Institute||August 1 2021||Not Applicable|
|NCT04642391||Not yet recruiting||Drug: Cosyntropin||Cirrhosis|Adrenal Insufficiency||University of Virginia||July 1 2021||--|
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