For research use only.
Catalog No.S1764 Synonyms: Rimactane,Rifampicin
CAS No. 13292-46-1
Rifampin (Rimactane, Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.
Selleck's Rifampin has been cited by 7 publications
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Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors
|Description||Rifampin (Rimactane, Rifampicin) is a DNA-dependent RNA polymerase inhibitor, used to treat a number of bacterial infections.|
Rifampin inhibits IκBα degradation and mitogen-activated protein kinase (MAPK) phosphorylation. Rifampin is found to bind to human MD-2 in a Rifampin and MD-2 concentration-dependent manner. Rifampin inhibits NF-κB activation induced by LPS (20 ng/ml) in a dose-dependent manner, with an IC50 of 44.1 μM in Blue hTLR4 293 cells (A) and immunocompetent microgial BV-2 cell. Rifampin (50 μM) suppresses NF-κB activation at varying LPS doses, and the maximum NF-κB level induced by LPS in the presence of Rifampin (50 μM) is much lower than that in the absence of Rifampin. Rifampin inhibits NO production induced by LPS (200 ng/ml) in a dose-dependent manner in BV-2 cells, with an IC50 of 21.2 μM. Rifampin suppresses LPS induced TNF-α and IL-1β production in both microglia BV-2 and RAW 264.7 macrophage cells. Rifampin-inhibiting innate immune signaling is independent of the pregnane X receptor NR1I2.  Rifampin combined with polyester vascular prostheses (PVP) functionalised with cyclodextrin (PVP-CD) results in significant bacterial adhesion reduction and growth inhibition against Gram-positive bacteria.  Rifampin (50 μg/mL) significantly reduces the CFU counts of stationary-phase cultures and reduces the CFU counts of the log-phase culture to zero. Rifampin is particularly suitable since it is bactericidal and starts killing M. tuberculosis within an hour of exposure. 
|In vitro||DMSO||100 mg/mL (121.51 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
In vivo Formulation Calculator (Clear solution)
|Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)|
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|% DMSO % % Tween 80 % ddH2O|
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
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Clinical Trial Information
|NCT Number||Recruitment||interventions||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT04301310||Not yet recruiting||Drug: BMS-986235 (Treatment A)|Drug: Rifampin (Treatment B)||Healthy Participants||Bristol-Myers Squibb||May 15 2020||Phase 1|
|NCT04537715||Recruiting||Drug: Tazemetostat|Drug: Itraconazole|Drug: Rifampin||All Malignancies|Advanced Malignancies|Hematologic Malignancy|Solid Tumor|Follicular Lymphoma (FL)|Non-Hodgkin Lymphoma (NHL)|Diffuse Large B-Cell Lymphoma (DLBCL)|Epithelioid Sarcoma (ES)|Synovial Sarcoma|Renal Medullary Carcinoma|Mesothelioma|Rhabdoid Tumor||Epizyme Inc.||April 23 2020||Phase 1|
|NCT04312282||Active not recruiting||Drug: Tesetaxel|Drug: Itraconazole|Drug: Rifampin||Advanced Solid Tumors||Odonate Therapeutics Inc.||March 6 2020||Phase 1|
|NCT04166669||Completed||Drug: APX001|Drug: Itraconazole|Drug: Rifampin||Fungal Infection||Amplyx Pharmaceuticals||November 12 2019||Phase 1|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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