Novobiocin Sodium (NSC 2382)

Catalog No.S2492 Synonyms: Albamycin, Cathomycin

For research use only.

Novobiocin Sodium (NSC 2382, Albamycin, Cathomycin) is an aminocoumarin antibiotic that targets bacterial DNA gyrase (TopoIV), used to treat susceptible gram positive bacteria.

Novobiocin Sodium (NSC 2382) Chemical Structure

CAS No. 1476-53-5

Selleck's Novobiocin Sodium (NSC 2382) has been cited by 5 Publications

1 Customer Review

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Novobiocin Sodium (NSC 2382, Albamycin, Cathomycin) is an aminocoumarin antibiotic that targets bacterial DNA gyrase (TopoIV), used to treat susceptible gram positive bacteria.
Targets
Topoisomerase II [1] Topoisomerase IV [1]
In vitro

Novobiocin also interacts with Hsp90, altering the affinity of the chaperone for geldanamycin and radicicol and causing in vitro and in vivo depletion of key regulatory Hsp90-dependent kinases including v-Src, Raf-1, and p185(ErbB2). Novobiocin interferes with association of the co-chaperones Hsc70 and p23 with Hsp90. [1] Novobiocin specifically inhibits the maturation of the heme-regulated eIF2alpha kinase (HRI) in a concentration-dependent manner. Novobiocin induces the dissociation of Hsp90 and Cdc37 from immature HRI, while the Hsp90 cochaperones p23, FKBP52, and protein phosphatase 5 remained associated with immature HRI. [2] Novobiocin causes morphological and biochemical changes which lead to induction of cell death exhibiting characteristic features of metazoan apoptosis. [3] Novobiocin, a HSP90 inhibitor, could decrease the expression of SMYD3 and dose dependently inhibit the proliferation and migration of MDA-MB-231 human breast cancer cells. Novobiocin can inhibit the migration of breast cancer cells and such event may involve the downregulation of SMYD3. [4] Novobiocin, an aminocoumarin antibiotic, interferes with heat shock protein 90 and hypoxia inducible factor dependent gene expression and thus compromises cell survival. Novobiocin (500 礛) results in a significant increase of [Ca(2+)]i, decrease of forward scatter, increase of annexin-V-binding and enhances ceramide formation. Novobiocin stimulates eryptosis, an effect at least in part due to entry of extracellular Ca(2+) and formation of ceramide. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7-MX NVrMUok6TnWwY4Tpc44h[XO|YYm= NFnHNWpVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCvaYTvfIFvfHKxbnWg[YZndHW6IHnuJGJEWlBvZYjwdoV{e2mwZzDNR2Y4NU2[IHPlcIx{NCCLQ{WwQVI2|ryP MlvDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2NkC3PVgoRjF4NE[wO|k5RC:jPh?=
MELN M1vXNWZ2dmO2aX;uJIF{e2G7 MYKyNFAhfU1? NXGwXpVIOThiaILz MXHJcohq[mm2aX;uJI9nKGW|dILh[IlwdC2rbnT1Z4VlKEWULX3l[IlifGWmIITyZY5{[3KrcITpc44hcW5iTVXMUkBk\WyuczDifUBu\WG|dYLpcochdHWlaX\ldoF{\SCjY4Tpeol1gSCjdDCyNFAhfU1iYX\0[ZIhOThiaILz M{C5XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe5NlY{Lz5zN{m3PVI3OzxxYU6=
SH-SY5Y NHHjZ2tP\XW{b4Dyc5Rm[3Srb36gZZN{[Xl? MVzO[ZVzd3C{b4TlZ5Rqd25iYXfhbY5{fCCkZYThMYFugWyxaXSgdIVxfGmmZTCxMVQzNWmwZIXj[YQhfG:6aXPpeJkhcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDhd5Nme3OnZDDhd{Bt[WO2YYTlJIRmcHmmcn;n[Y5ie2VicnXs[YF{\SxiRVO1NF0xNjB2OEO5{txO NVG1NXlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN|g5PTlpPkG5NVM5QDV7PD;hQi=>
SH-SY5Y MUfO[ZVzd3C{b4TlZ5Rqd25iYYPzZZk> M1v1[VExKG6P NEHjfWJP\XW{b4Dyc5Rm[3Srb36gZYdicW6|dDDi[ZRiNWGveXzvbYQheGWydHnk[UAyNTR{LXnu[JVk\WRidH;4bYNqfHliaX6gdoV1cW6xaXOgZYNq\C2maX\m[ZJmdnSrYYTl[EBpfW2jbjDTTE1UYTW\IHPlcIx{KGG|c3Xzd4VlKGG|IHzhZ5RifGViZHXofYRzd2enbnHz[UBz\WynYYPlJIF1KDFyIH7N NWq5TIhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxN|g5PTlpPkG5NVM5QDV7PD;hQi=>
SH-SY5Y Mn;IUoV2em:ycn;0[YN1cW:wIHHzd4F6 M3PWSm5mfXKxcILveIVkfGmxbjDh[4FqdnO2IHLleIEu[W27bH;p[EBx\XC2aXTlJFEuPDJvaX7keYNm\CC2b4jpZ4l1gSCrbjDy[ZRqdm:rYzDhZ4llNWSrZn\ldoVvfGmjdHXkJIh2dWGwIGPIMXN[PVliY3XscJMh[XO|ZYPz[YQh[XNibHHjeIF1\SCmZXj5[JJw\2WwYYPlJJJmdGWjc3W= MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzM{i4OVkoRjF7MUO4PFU6RC:jPh?=
LNCaP-LN3 MWHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFTzfXpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzOR4FRNUyQMzDj[YxteyxiSVO1NF0yNjB3zszN MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF{OUm4Nkc,OjFzMkm5PFI9N2F-
yeast PP30 NGTjS5BHfW6ldHnvckBie3OjeR?= M2HiTFExOCC3TR?= M3zSTFMhcHK| M3roWmlvcGmkaYTpc44hd2ZiaIXtZY4hUFOSOUDhcJBp[SCneIDy[ZN{\WRiaX6gfYVie3RiUGCzNEBk\WyuczDjc{1mgHC{ZYPzbY5oKEiVRj3sZYNbKHKncH;yeIVzKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcGWjdDDzeJJme3NvaX7keYNm\CC{ZYPwc45{\SCjdDCxNFAhfU1iYX\0[ZIhOyCqcoO= NHjpWos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGyPVk5Oid-MkGxNlk6QDJ:L3G+
Sf9 Ml70SpVv[3Srb36gZZN{[Xl? NH3Rc|JKdmirYnn0bY9vKG:oIFXzZ4hmemmlaHnhJINwdGliSHnzOk11[WepZXSgVIFzSy:SYYLFJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDT[lkh[2WubIOgZpkh\2WuIHXs[YN1em:yaH;y[ZNqeyxiSVO1NF0xNjJzzszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ|NUK0NUc,OjF{M{WyOFE9N2F-
SKBR3 NVHaXZZJTnWwY4Tpc44h[XO|YYm= NX7EZVFLOTByMDD1US=> NFrLV|MyQCCqcoO= NFHDO4ZKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKFONQmKzJINmdGy|IHHzd4V{e2WmIHHzJIFo\3KnZ3H0bY9vKG:oIHnueJJi[2WubIXsZZIhUEWUMjDheEAyODByIIXNJIFnfGW{IEG4JIhzeyCkeTDERXBKKHO2YXnubY5oNWKjc3XkJIludXWwb3\seY9z\XOlZX7j[UBucWO{b4Pjc5Bq[yCjbnHsfZNqew>? M1PNVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEW5O|c4Lz5{M{i1PVc4PzxxYU6=
BL21 (DE3) pLysS NImxNJNHfW6ldHnvckBie3OjeR?= NFnYT5YyODBibXnudy=> Mom2TY5pcWKrdHnvckBw\iB4LVjpd{11[WepZXSgUZlkd2KjY4Tldol2dSC|bXXncYF1cXNiR4nyRkBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIIDMfZNUKGOnbHzzJIlv[3WkYYTl[EBnd3JiMUCwJI1qdnNiaX6gdJJme2WwY3Wgc4YhSVSSIHL5JI1idGGlaHn0[UBoemWnbjDkfYUh[mG|ZXSgRXRRKGG|c3H5MEBKSzVyPUCuNVjPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{OUG3NUc,OjVzMkmxO|E9N2F-
BL21 (DE3) pLysS Mlv1SpVv[3Srb36gZZN{[Xl? NUfSeJhxOTByIH3pcpM> MkCyTY5pcWKrdHnvckBw\iCPeXPvZoFkfGW{aYXtJJNu\WevYYTpd{BIgXKEIFHUVIF{\SCjY4Tpeol1gSCneIDy[ZN{\WRiaX6gSZNkcGW{aXPobYEh[2:uaTDCUFIyKCiGRUOpJJBNgXOVIHPlcIx{KGGodHXyJFExOCCvaX7zJIJ6KGmwb4LnZY5q[yCyaH;zdIhifGVicnXs[YF{\SCmZYTlZ5Rqd25iYnHz[YQhdWGuYXPobZRmKGe{ZXXuJJJm[WenboSgZZN{[XluIFnDOVA:OC5zON88US=> Mkf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MEGxOVEoRjJ3OECxNVUyRC:jPh?=
BL21 (DE3) pLysS M2W2[2Z2dmO2aX;uJIF{e2G7 M33Nc|ExOCCvaX7z NEfHbWFKdmirYnn0bY9vKG:oIF35Z49j[WO2ZYLpeY0he22nZ33heIl{KESQQTDnfZJie2ViQjDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIDjESVMqKHCOeYPTJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCDVGDhd4Uh[WO2aY\peJkhcW6ldXLheIVlKG[xcjCxNFAhdWmwczDifUBqdm:{Z3HubYMheGixc4DoZZRmKGSndHXjeIlwdiCjc4PhfUwhUUN3ME2wMlE5|ryP MmfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ|MUiwOVQoRjJ4M{G4NFU1RC:jPh?=
BL21 (DE3) pLysS NWe2OmFPTnWwY4Tpc44h[XO|YYm= MnnvNVIxKG2rboO= M1rh[2lvcGmkaYTpc44hd2ZiTYnjc4Ji[3Sncnn1cUB{dWWpbXH0bZMhOTV3IE\IbZMufGGpZ3XkJGRPSSCpeYLhd4UhSiClYYThcJl1cWNiZH;tZYlvKEGWUHHz[UBi[3Srdnn0fUBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCETEKxJEhFTTNrIIDMfZNUKGOnbHzzJIF{e2W|c3XkJIF{KGmwb4LnZY5q[yCyaH;zdIhifGVicnXs[YF{\SCjZoTldkAyOjBibXnud{BqdiCycnXz[Y5k\SCxZjDBWHAh[nlibXHsZYNpcXSnIHfy[YVvKGS7ZTDiZZNm\CxiSVO1NF0xNjB2Nt88US=> MmLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd|N{G5NlUoRjJ5M{exPVI2RC:jPh?=

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 634.61
Formula

C31H35N2O11.Na

CAS No. 1476-53-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C=CC2=C1OC(=O)C(=C2O)NC(=O)C3=CC(=C(C=C3)[O-])CC=C(C)C)OC4C(C(C(C(O4)(C)C)OC)OC(=O)N)O.[Na+]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) supplier | purchase Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) cost | Novobiocin Sodium (NSC 2382) manufacturer | order Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) distributor