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Bleomycin sulfate DNA Synthesis inhibitor

Name: Bleomycin sulfate
Brand: Selleck Chemicals
SKU: S1214
Availability: InStock
Rating: 5 (163 reviews)

Cat.No.S1214

Bleomycin sulfate is a glycopeptide antibiotic and a DNA synthesis inhibitor, as well as an anticancer agent for squamous cell carcinomas (SCC) with an IC50 of 4 nM in UT-SCC-19A cells. Bleomycin sulfate is a DNA synthesis inhibitor. Bleomycin (NSC125066) sulfate can be used to induce animal models of pulmonary fibrosis.

Chemical Structure

Molecular Weight: 1512.62

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Quality Control

Batch: Purity: 99.97%

99.97

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Related Targets	CDK HSP K-Ras PD-1/PD-L1 ROCK Wee1 Microtubule Associated Ras Aurora Kinase Casein Kinase
Other DNA/RNA Synthesis Inhibitors	B02 CX-5461 (Pidnarulex) SCR7 EED226 Favipiravir (T-705) RK-33 Carmofur Triapine (3-AP) BMH-21 YK-4-279

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
F98	Growth Inhibition Assay	0–1000 μm	24 h	inhibits cell growth dose dependently	26088711
POLK WT	Growth Inhibition Assay			IC50=11.7 ± 1.7 μM	26031400
POLK KO	Growth Inhibition Assay			IC50=4.03 ± 1.92 μM	26031400
POLK CD	Growth Inhibition Assay			IC50=5.59 ± 1.00 μM	26031400
RLE/Abca3	Function Assay	0.4 μM	144 h	induces a morphological change from epithelial-like morphology to a wide, spread-out morphology	25760538
PMCs	Function Assay	0.1–2 μg/ml	24 h	causes a dose-dependent increase in the protein level of collagen-I	25595642
PMCs	Function Assay	0.2 μg/ml	24–72 h	induces increases in vimentin and α-SMA protein	25595642
PMCs	Function Assay	0.2 μg/ml	72 h	induces decreases in cytokeratin-8 and E-cadherin protein	25595642
PMCs	Function Assay	0.2 μg/ml	12/24/48 h	increases cell migration	25595642
IMR-32	Growth Inhibition Assay	7.5−120 µg/ml	12−48 h	IC50=60 µg/ml at the end of 24 hrs of incubation	25536345
HT1080	Growth Inhibition Assay	1 μM	24 h	leads to a G2/M delay	25518961
HT1080	Function Assay	1 μM	24 h	induces a potent global DNA damage response	25518961
HDFn	Cytotoxicity Assay		72 h	IC50=9.31%	25276792
THP-1	Cytotoxicity Assay		72 h	IC50=4.77%	25276792
HT-29	Cytotoxicity Assay		72 h	IC50=11.49%	25276792
HCT116	Cytotoxicity Assay		72 h	IC50=11.34%	25276792
A431	Growth Inhibition Assay	50-200 IU	48 h	inhibits cell growth dose dependently	25101299
T24	Growth Inhibition Assay	50-200 IU	48 h	inhibits cell growth dose dependently	25101299
AY-27	Growth Inhibition Assay	50-200 IU	48 h	inhibits cell growth dose dependently	25101299
A549	Function Assay	1 µg/mL	0-48 h	increases the expression of OPN mRNA	24963635
MLE12	Function Assay	1 µg/mL	0-48 h	increases the expression of OPN mRNA	24963635
Jurkat	Growth Inhibition Assay	20 μm	24 h	arrests the cell cycle at the G2 phase	24916893
HeLa	Growth Inhibition Assay			IC50=10.2 μM	24732397
C18-4	Growth Inhibition Assay	0-100 μM		inhibits cell growth dose dependently	24571982
BMG-1	Growth Inhibition Assay	40/80 μg/m	3 h	inhibits cell growth dose dependently	23906726
A459	Apoptosis Assay	10 mU	48 h	induces apoptosis	23902766
MOCK	Apoptosis Assay	10 mU	48 h	induces apoptosis	23902766
MMP1	Apoptosis Assay	10 mU	48 h	induces apoptosis	23902766
A549	Growth Inhibition Assay	200 μM	24 h	decreases cell viability significantly	23805793
HCT-116	Apoptosis Assay	3/7.5/15 μg/ml	48 h	reduces the level of BLH at high concentration	23708668
HeLa	Apoptosis Assay	3/7.5/15 μg/ml	48 h	reduces the level of BLH at high concentration	23708668
HCT116	Growth Inhibition Assay	0-100 μg/ml	48 h	inhibits cell growth dose dependently	23518201
NCM460	Growth Inhibition Assay	0-100 μg/ml	48 h	inhibits cell growth dose dependently	23518201
NT2	Growth Inhibition Assay		24 h	LD50=400 µg/ml	23386420
NT2	Growth Inhibition Assay		48 h	LD50=100 µg/ml	23386420
NT2	Growth Inhibition Assay		72 h	LD50=20 µg/ml	23386420
NT2	Apoptosis Assay	400 µg/ml	24 h	induces significant increases in caspase-3,8,9 activity	23386420
NTera-2	Function Assay	120 μg/ml	72 h	significantly increases 8-isoprostane levels	22825355
NCCIT	Function Assay	120 μg/ml	72 h	significantly increases 8-isoprostane levels	22825355
NTera-2	Function Assay	120 μg/ml	72 h	significantly increases TBARS levels	22825355
NCCIT	Function Assay	120 μg/ml	72 h	significantly increases TBARS levels	22825355
NCCIT	Function Assay	120 μg/ml	72 h	significantly decreases GSH levels	22825355
NTera-2	Function Assay	120 μg/ml	72 h	significantly increases Protein carbonyl levels	22825355
NCCIT	Function Assay	120 μg/ml	72 h	significantly increases Protein carbonyl levels	22825355
NTera-2	Function Assay	120 μg/ml	72 h	significantly increases LPO levels	22825355
NCCIT	Function Assay	120 μg/ml	72 h	significantly increases LPO levels	22825355
MDA-MB-468	Growth Inhibition Assay	0–200 μg/mL	24 h	inhibits cell growth dose dependently	22819196
231-H2N	Growth Inhibition Assay	0–200 μg/mL	24 h	inhibits cell growth dose dependently	22819196
A549	Function Assay	200/400 μM		results in a dose-responsive increase in ROS	22773697
A549	Function Assay	0-400 μM	2-48 h	causes more mtDNA than nDNA damage	22773697
A549	Function Assay	100 μM	0–48 h	causes caspase-3 activation	22773697
A549	Function Assay	100 μM	0–48 h	causes PKD1 cleavage	22773697
A549	Function Assay	100 μM	0–48 h	causes mitochondrial localization of Bax	22773697
A549	Function Assay	100 μM	0–48 h	decreases MMP and ATP levels	22773697
MCF-7/Her-18	Growth Inhibition Assay	0.42-1700 μg/mL	24 h	inhibits cell growth dose dependently	22621404
MCF-7	PDT/PCI Treatment	0.25 μg/ml.	1-4 h	shows significant (P < 0.05) cytotoxic effect	22591284
MDA-MB-231	PDT/PCI Treatment	0.25 μg/ml.	1-4 h	shows significant (P < 0.05) cytotoxic effect	22591284
MDA-MB-235	PDT/PCI Treatment	0.25 μg/ml.	1-4 h	shows significant (P < 0.05) cytotoxic effect	22591284
MCF-7	Growth Inhibition Assay	0-2 μg/ml.	24/48 h	EC50=1.2 μg/mL	22591284
NCCIT	Growth Inhibition Assay	20–140 μg/ml	72 h	LD50=120 μg/ml	22562160
NCCIT	Function Assay		72 h	significantly increases caspase-3 activity	22562160
NCCIT	Function Assay		72 h	significantly increases caspase-8 activity	22562160
NCCIT	Function Assay		72 h	significantly increases caspase-9 activity	22562160
NCCIT	Function Assay		72 h	significantly increases BAX level	22562160
NCCIT	Function Assay		72 h	significantly decreases the Bcl-2 content	22562160
NCCIT	Function Assay		72 h	significantly decreases the Cyt-c level	22562160
HeLa	Function Assay	30/70 μg/ml	24 h	increases the mRNA levels of p53，ATM,p21 and GADD45	22487937
MCF-7	Function Assay	30/70 μg/ml	24 h	increases the mRNA levels of p53，ATM,p21 and GADD45	22487937
HeLa	Cytotoxicity Assay	30/70 μg/ml	24 h	induces highly significant levels of fragmented DNA release into the cytoplasm	22487937
MCF-7	Cytotoxicity Assay	30/70 μg/ml	24 h	induces highly significant levels of fragmented DNA release into the cytoplasm	22487937
NT2	Growth Inhibition Assay	100-600 μg/ml	24 h	IC50=400 µg/ml	22469952
NT2	Function Assay	400 μg/ml	24 h	significantly increases caspase-3,8,9 activity	22469952
NT2	Function Assay	400 μg/ml	24 h	significantly increases BAX level	22469952
NT2	Function Assay	400 μg/ml	24 h	significantly decreases Bcl-2 level	22469952
CHO	Function Assay	2.5 μg/ml	18 h/6 d	induces persistence of chromosome damage	22230195
Jurkat	Growth Inhibition Assay	0-100 μg/ml	24 h	arrests the cell cycle at the G2 phase	22223332
Jurkat	Function Assay	30 μg/ml	24 h	increases the protein levels of cyclins D3, A and B1	22223332
Jurkat	Growth Inhibition Assay	0-100 μg/ml	24 h	inhibits cell proliferation in a dose-dependent manner with an IC50 value of 100μg/mL	22223332
Jurkat	Function Assay	30 μg/ml	24 h	increases the effect of pycnidione on phosphorylation levels of histone H2A.X	22223332
U2OS EGFPnls	Growth Inhibition Assay	0-2 μg/ml	24 h	inhibits cell growth dose dependently	21811007
U2OS KuEnls	Growth Inhibition Assay	0-2 μg/ml	24 h	inhibits cell growth dose dependently	21811007
MCF-7	Growth Inhibition Assay	0-400 μM	72 h	LC50=151.8 μM	21703291
MCF-7/Adr	Growth Inhibition Assay	0-400 μM	72 h	LC50=58.5 μM	21703291
WI-38	Function Assay	0/40/80 μg/ml	3 h	induces a concentration-dependent increase in the MN frequency	21599612
hBMSC	Function Assay	0/40/80 μg/ml	3 h	induces a concentration-dependent increase in the MN frequency	21599612
NCI-H23	Function Assay	0/40/80 μg/ml	3 h	induces a concentration-dependent increase in the MN frequency	21599612
A-549	Function Assay	0/40/80 μg/ml	3 h	induces a concentration-dependent increase in the MN frequency	21599612
PBL	Function Assay	0/40/80 μg/ml	3 h	induces a concentration-dependent increase in the MN frequency	21599612
pol β −/−	Growth Inhibition Assay	0.25-200 μg/ml	24 h	decreases cell viability with increasing concentrations	21251944
pol β +/+	Growth Inhibition Assay	0.25-200 μg/ml	24 h	decreases cell viability with increasing concentrations	21251944
pol β −/−	Function Assay	0-2.5 μg/ml	2 h	causes DNA damage	21251944
pol β +/+	Function Assay	0-2.5 μg/ml	2 h	causes DNA damage	21251944
pol β −/−	Function Assay	0-5 μg/ml	48 h	causes micronucleus dose and time dependently	21251944
pol β +/+	Function Assay	0-5 μg/ml	48 h	causes micronucleus dose and time dependently	21251944
TK6	Growth Inhibition Assay	25-150 μg/ml	96 h	inhibits cell growth dose dependently	21081487
TK6 sLUC+Apn1	Growth Inhibition Assay	25-150 μg/ml	96 h	inhibits cell growth dose dependently	21081487
TK6 sAPE1+Apn1	Growth Inhibition Assay	25-150 μg/ml	96 h	significantly inhibits cell growth dose dependently	21081487
HCT116	Growth Inhibition Assay	25-150 μg/ml	96 h	inhibits cell growth dose dependently	21081487
HCT116 sLUC+Apn1	Growth Inhibition Assay	25-150 μg/ml	96 h	inhibits cell growth dose dependently	21081487
HCT116 sAPE1+Apn1	Growth Inhibition Assay	25-150 μg/ml	96 h	significantly inhibits cell growth dose dependently	21081487
2SC/20	Function Assay		72 h	IC50 = 0.517 μM	12431060
HET-SR-2SC-LNM	Function Assay		72 h	IC50 = 0.519 μM	12431060
HepG2	Growth Inhibition Assay			GI50 = 1 μM	18068362
MCF-7	Function Assay		24 h	IC50 = 53 μM	12477361
COLO-668	Growth Inhibition Assay			IC50 = 0.0000218 μM	SANGER
CAL-51	Growth Inhibition Assay			IC50 = 0.000099 μM	SANGER
A427	Growth Inhibition Assay			IC50 = 0.000118 μM	SANGER
RMG-I	Growth Inhibition Assay			IC50 = 0.0001319 μM	SANGER
HUTU-80	Growth Inhibition Assay			IC50 = 0.0001358 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay			IC50 = 0.0001391 μM	SANGER
IST-SL2	Growth Inhibition Assay			IC50 = 0.0001732 μM	SANGER
CAL-85-1	Growth Inhibition Assay			IC50 = 0.0001777 μM	SANGER
SUP-T1	Growth Inhibition Assay			IC50 = 0.000251 μM	SANGER
HCC1937	Growth Inhibition Assay			IC50 = 0.000254 μM	SANGER
CAL-39	Growth Inhibition Assay			IC50 = 0.0003147 μM	SANGER
HAL-01	Growth Inhibition Assay			IC50 = 0.0004527 μM	SANGER
OVCAR-8	Growth Inhibition Assay			IC50 = 0.0004763 μM	SANGER
CESS	Growth Inhibition Assay			IC50 = 0.0005457 μM	SANGER
NCI-H650	Growth Inhibition Assay			IC50 = 0.0005873 μM	SANGER
MKN28	Growth Inhibition Assay			IC50 = 0.00108 μM	SANGER
RS4-11	Growth Inhibition Assay			IC50 = 0.001643 μM	SANGER
MFH-ino	Growth Inhibition Assay			IC50 = 0.002646 μM	SANGER
KYSE-150	Growth Inhibition Assay			IC50 = 0.002647 μM	SANGER
KGN	Growth Inhibition Assay			IC50 = 0.003712 μM	SANGER
HOP-62	Growth Inhibition Assay			IC50 = 0.004134 μM	SANGER
LU-99A	Growth Inhibition Assay			IC50 = 0.00422 μM	SANGER
RT4	Growth Inhibition Assay			IC50 = 0.005856 μM	SANGER
SK-UT-1	Growth Inhibition Assay			IC50 = 0.006293 μM	SANGER
HCC2998	Growth Inhibition Assay			IC50 = 0.006592 μM	SANGER
GT3TKB	Growth Inhibition Assay			IC50 = 0.00685 μM	SANGER
EW-16	Growth Inhibition Assay			IC50 = 0.006853 μM	SANGER
NKM-1	Growth Inhibition Assay			IC50 = 0.007647 μM	SANGER
HCC1806	Growth Inhibition Assay			IC50 = 0.008543 μM	SANGER
SW954	Growth Inhibition Assay			IC50 = 0.009572 μM	SANGER
MV-4-11	Growth Inhibition Assay			IC50 = 0.009908 μM	SANGER
LOUCY	Growth Inhibition Assay			IC50 = 0.009935 μM	SANGER
KS-1	Growth Inhibition Assay			IC50 = 0.01084 μM	SANGER
NMC-G1	Growth Inhibition Assay			IC50 = 0.01185 μM	SANGER
REH	Growth Inhibition Assay			IC50 = 0.01339 μM	SANGER
NCI-H2342	Growth Inhibition Assay			IC50 = 0.01383 μM	SANGER
BFTC-909	Growth Inhibition Assay			IC50 = 0.01383 μM	SANGER
EFM-19	Growth Inhibition Assay			IC50 = 0.01477 μM	SANGER
NCI-H1703	Growth Inhibition Assay			IC50 = 0.01612 μM	SANGER
BB30-HNC	Growth Inhibition Assay			IC50 = 0.01634 μM	SANGER
NCI-H1734	Growth Inhibition Assay			IC50 = 0.01742 μM	SANGER
SF295	Growth Inhibition Assay			IC50 = 0.02139 μM	SANGER
MKN45	Growth Inhibition Assay			IC50 = 0.02481 μM	SANGER
ES1	Growth Inhibition Assay			IC50 = 0.0267 μM	SANGER
NEC8	Growth Inhibition Assay			IC50 = 0.02685 μM	SANGER
CTB-1	Growth Inhibition Assay			IC50 = 0.02694 μM	SANGER
5637	Growth Inhibition Assay			IC50 = 0.02897 μM	SANGER
SR	Growth Inhibition Assay			IC50 = 0.02967 μM	SANGER
HCC38	Growth Inhibition Assay			IC50 = 0.03124 μM	SANGER
LB1047-RCC	Growth Inhibition Assay			IC50 = 0.03255 μM	SANGER
ES4	Growth Inhibition Assay			IC50 = 0.03826 μM	SANGER
MHH-ES-1	Growth Inhibition Assay			IC50 = 0.04357 μM	SANGER
LS-411N	Growth Inhibition Assay			IC50 = 0.05276 μM	SANGER
HT-144	Growth Inhibition Assay			IC50 = 0.05514 μM	SANGER
BPH-1	Growth Inhibition Assay			IC50 = 0.05909 μM	SANGER
HMV-II	Growth Inhibition Assay			IC50 = 0.06974 μM	SANGER
A375	Growth Inhibition Assay			IC50 = 0.0711 μM	SANGER
LoVo	Growth Inhibition Assay			IC50 = 0.07117 μM	SANGER
COR-L23	Growth Inhibition Assay			IC50 = 0.07548 μM	SANGER
G-402	Growth Inhibition Assay			IC50 = 0.07651 μM	SANGER
CCRF-CEM	Growth Inhibition Assay			IC50 = 0.07869 μM	SANGER
MKN1	Growth Inhibition Assay			IC50 = 0.07965 μM	SANGER
LC-2-ad	Growth Inhibition Assay			IC50 = 0.08036 μM	SANGER
ETK-1	Growth Inhibition Assay			IC50 = 0.0805 μM	SANGER
786-0	Growth Inhibition Assay			IC50 = 0.08084 μM	SANGER
SW13	Growth Inhibition Assay			IC50 = 0.08162 μM	SANGER
Daoy	Growth Inhibition Assay			IC50 = 0.08304 μM	SANGER
G-361	Growth Inhibition Assay			IC50 = 0.08831 μM	SANGER
HCT-15	Growth Inhibition Assay			IC50 = 0.0951 μM	SANGER
ARH-77	Growth Inhibition Assay			IC50 = 0.10452 μM	SANGER
NCI-H1299	Growth Inhibition Assay			IC50 = 0.10753 μM	SANGER
MEL-JUSO	Growth Inhibition Assay			IC50 = 0.10855 μM	SANGER
MIA-PaCa-2	Growth Inhibition Assay			IC50 = 0.11074 μM	SANGER
769-P	Growth Inhibition Assay			IC50 = 0.1228 μM	SANGER
MZ7-mel	Growth Inhibition Assay			IC50 = 0.1305 μM	SANGER
KG-1	Growth Inhibition Assay			IC50 = 0.13057 μM	SANGER
23132-87	Growth Inhibition Assay			IC50 = 0.13146 μM	SANGER
ML-2	Growth Inhibition Assay			IC50 = 0.13166 μM	SANGER
BOKU	Growth Inhibition Assay			IC50 = 0.13698 μM	SANGER
SW1573	Growth Inhibition Assay			IC50 = 0.13715 μM	SANGER
IMR-5	Growth Inhibition Assay			IC50 = 0.13736 μM	SANGER
PSN1	Growth Inhibition Assay			IC50 = 0.13888 μM	SANGER
LCLC-103H	Growth Inhibition Assay			IC50 = 0.14398 μM	SANGER
IGROV-1	Growth Inhibition Assay			IC50 = 0.14455 μM	SANGER
CHP-212	Growth Inhibition Assay			IC50 = 0.14774 μM	SANGER
RH-1	Growth Inhibition Assay			IC50 = 0.15221 μM	SANGER
MFE-296	Growth Inhibition Assay			IC50 = 0.15324 μM	SANGER
NCI-H2170	Growth Inhibition Assay			IC50 = 0.16242 μM	SANGER
Saos-2	Growth Inhibition Assay			IC50 = 0.17196 μM	SANGER
LB996-RCC	Growth Inhibition Assay			IC50 = 0.17534 μM	SANGER
LB2518-MEL	Growth Inhibition Assay			IC50 = 0.18225 μM	SANGER
PC-14	Growth Inhibition Assay			IC50 = 0.18269 μM	SANGER
ESS-1	Growth Inhibition Assay			IC50 = 0.18744 μM	SANGER
CAL-54	Growth Inhibition Assay			IC50 = 0.19067 μM	SANGER
A204	Growth Inhibition Assay			IC50 = 0.20037 μM	SANGER
639-V	Growth Inhibition Assay			IC50 = 0.21956 μM	SANGER
A2780	Growth Inhibition Assay			IC50 = 0.22019 μM	SANGER
MZ1-PC	Growth Inhibition Assay			IC50 = 0.22434 μM	SANGER
GDM-1	Growth Inhibition Assay			IC50 = 0.22845 μM	SANGER
HT-1080	Growth Inhibition Assay			IC50 = 0.23357 μM	SANGER
SK-CO-1	Growth Inhibition Assay			IC50 = 0.24652 μM	SANGER
SW1783	Growth Inhibition Assay			IC50 = 0.24925 μM	SANGER
PANC-10-05	Growth Inhibition Assay			IC50 = 0.25606 μM	SANGER
CAL-12T	Growth Inhibition Assay			IC50 = 0.25851 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay			IC50 = 0.26047 μM	SANGER
MDA-MB-231	Growth Inhibition Assay			IC50 = 0.27307 μM	SANGER
IST-MEL1	Growth Inhibition Assay			IC50 = 0.27975 μM	SANGER
GAMG	Growth Inhibition Assay			IC50 = 0.28935 μM	SANGER
EW-13	Growth Inhibition Assay			IC50 = 0.29208 μM	SANGER
SW1710	Growth Inhibition Assay			IC50 = 0.29533 μM	SANGER
C3A	Growth Inhibition Assay			IC50 = 0.29644 μM	SANGER
PANC-03-27	Growth Inhibition Assay			IC50 = 0.31639 μM	SANGER
H4	Growth Inhibition Assay			IC50 = 0.32074 μM	SANGER
NCI-H358	Growth Inhibition Assay			IC50 = 0.37271 μM	SANGER
ACHN	Growth Inhibition Assay			IC50 = 0.37463 μM	SANGER
TE-8	Growth Inhibition Assay			IC50 = 0.37669 μM	SANGER
SK-MEL-30	Growth Inhibition Assay			IC50 = 0.37688 μM	SANGER
ES7	Growth Inhibition Assay			IC50 = 0.37754 μM	SANGER
DoTc2-4510	Growth Inhibition Assay			IC50 = 0.37834 μM	SANGER
C-4-II	Growth Inhibition Assay			IC50 = 0.38131 μM	SANGER
SNU-449	Growth Inhibition Assay			IC50 = 0.38851 μM	SANGER
GB-1	Growth Inhibition Assay			IC50 = 0.39011 μM	SANGER
SF539	Growth Inhibition Assay			IC50 = 0.39305 μM	SANGER
MEG-01	Growth Inhibition Assay			IC50 = 0.39549 μM	SANGER
BE-13	Growth Inhibition Assay			IC50 = 0.39872 μM	SANGER
ES6	Growth Inhibition Assay			IC50 = 0.40625 μM	SANGER
SJRH30	Growth Inhibition Assay			IC50 = 0.41411 μM	SANGER
SK-HEP-1	Growth Inhibition Assay			IC50 = 0.41493 μM	SANGER
SCC-25	Growth Inhibition Assay			IC50 = 0.41539 μM	SANGER
SW982	Growth Inhibition Assay			IC50 = 0.41743 μM	SANGER
NALM-6	Growth Inhibition Assay			IC50 = 0.42016 μM	SANGER
NCI-H522	Growth Inhibition Assay			IC50 = 0.42569 μM	SANGER
PF-382	Growth Inhibition Assay			IC50 = 0.42616 μM	SANGER
C-33-A	Growth Inhibition Assay			IC50 = 0.43178 μM	SANGER
GI-ME-N	Growth Inhibition Assay			IC50 = 0.44079 μM	SANGER
RPMI-6666	Growth Inhibition Assay			IC50 = 0.44505 μM	SANGER
LOXIMVI	Growth Inhibition Assay			IC50 = 0.45232 μM	SANGER
HCE-4	Growth Inhibition Assay			IC50 = 0.45316 μM	SANGER
DEL	Growth Inhibition Assay			IC50 = 0.47988 μM	SANGER
NCI-H1650	Growth Inhibition Assay			IC50 = 0.49938 μM	SANGER
HCC1395	Growth Inhibition Assay			IC50 = 0.50094 μM	SANGER
AGS	Growth Inhibition Assay			IC50 = 0.50253 μM	SANGER
NCI-H1651	Growth Inhibition Assay			IC50 = 0.5053 μM	SANGER
ES5	Growth Inhibition Assay			IC50 = 0.50539 μM	SANGER
NCI-H2122	Growth Inhibition Assay			IC50 = 0.50953 μM	SANGER
BC-3	Growth Inhibition Assay			IC50 = 0.521 μM	SANGER
RKO	Growth Inhibition Assay			IC50 = 0.52424 μM	SANGER
MHH-CALL-2	Growth Inhibition Assay			IC50 = 0.53464 μM	SANGER
HT-29	Growth Inhibition Assay			IC50 = 0.5355 μM	SANGER
SW48	Growth Inhibition Assay			IC50 = 0.54612 μM	SANGER
GOTO	Growth Inhibition Assay			IC50 = 0.54993 μM	SANGER
HD-MY-Z	Growth Inhibition Assay			IC50 = 0.5602 μM	SANGER
HOS	Growth Inhibition Assay			IC50 = 0.56023 μM	SANGER
EFO-27	Growth Inhibition Assay			IC50 = 0.57104 μM	SANGER
JVM-2	Growth Inhibition Assay			IC50 = 0.57224 μM	SANGER
SIG-M5	Growth Inhibition Assay			IC50 = 0.58605 μM	SANGER
HCC1569	Growth Inhibition Assay			IC50 = 0.60449 μM	SANGER
A101D	Growth Inhibition Assay			IC50 = 0.61972 μM	SANGER
CAKI-1	Growth Inhibition Assay			IC50 = 0.62043 μM	SANGER
SBC-5	Growth Inhibition Assay			IC50 = 0.62355 μM	SANGER
RPMI-2650	Growth Inhibition Assay			IC50 = 0.63315 μM	SANGER
QIMR-WIL	Growth Inhibition Assay			IC50 = 0.63656 μM	SANGER
NCI-H64	Growth Inhibition Assay			IC50 = 0.64101 μM	SANGER
NCI-SNU-5	Growth Inhibition Assay			IC50 = 0.64336 μM	SANGER
LXF-289	Growth Inhibition Assay			IC50 = 0.64425 μM	SANGER
HSC-3	Growth Inhibition Assay			IC50 = 0.68829 μM	SANGER
EB2	Growth Inhibition Assay			IC50 = 0.69103 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay			IC50 = 0.71228 μM	SANGER
KYSE-270	Growth Inhibition Assay			IC50 = 0.7169 μM	SANGER
A3-KAW	Growth Inhibition Assay			IC50 = 0.71692 μM	SANGER
TE-12	Growth Inhibition Assay			IC50 = 0.71885 μM	SANGER
MDA-MB-361	Growth Inhibition Assay			IC50 = 0.71909 μM	SANGER
T-24	Growth Inhibition Assay			IC50 = 0.71991 μM	SANGER
CHL-1	Growth Inhibition Assay			IC50 = 0.73154 μM	SANGER
LB2241-RCC	Growth Inhibition Assay			IC50 = 0.73825 μM	SANGER
NCI-H1437	Growth Inhibition Assay			IC50 = 0.74272 μM	SANGER
Ca9-22	Growth Inhibition Assay			IC50 = 0.74583 μM	SANGER
EGI-1	Growth Inhibition Assay			IC50 = 0.77232 μM	SANGER
J82	Growth Inhibition Assay			IC50 = 0.77431 μM	SANGER
TE-10	Growth Inhibition Assay			IC50 = 0.78312 μM	SANGER
Ca-Ski	Growth Inhibition Assay			IC50 = 0.79823 μM	SANGER
NCI-H292	Growth Inhibition Assay			IC50 = 0.80316 μM	SANGER
8305C	Growth Inhibition Assay			IC50 = 0.80431 μM	SANGER
A2058	Growth Inhibition Assay			IC50 = 0.80768 μM	SANGER
ACN	Growth Inhibition Assay			IC50 = 0.80834 μM	SANGER
DU-145	Growth Inhibition Assay			IC50 = 0.82255 μM	SANGER
SNG-M	Growth Inhibition Assay			IC50 = 0.83083 μM	SANGER
OAW-42	Growth Inhibition Assay			IC50 = 0.83182 μM	SANGER
MEL-HO	Growth Inhibition Assay			IC50 = 0.85021 μM	SANGER
ONS-76	Growth Inhibition Assay			IC50 = 0.85391 μM	SANGER
BV-173	Growth Inhibition Assay			IC50 = 0.85487 μM	SANGER
SNU-423	Growth Inhibition Assay			IC50 = 0.86739 μM	SANGER
MLMA	Growth Inhibition Assay			IC50 = 0.87048 μM	SANGER
A388	Growth Inhibition Assay			IC50 = 0.87718 μM	SANGER
NB69	Growth Inhibition Assay			IC50 = 0.92254 μM	SANGER
SNU-387	Growth Inhibition Assay			IC50 = 0.92472 μM	SANGER
U031	Growth Inhibition Assay			IC50 = 0.93436 μM	SANGER
NCI-H2228	Growth Inhibition Assay			IC50 = 0.95465 μM	SANGER
UM-UC-3	Growth Inhibition Assay			IC50 = 0.95561 μM	SANGER
ALL-PO	Growth Inhibition Assay			IC50 = 0.98627 μM	SANGER
HC-1	Growth Inhibition Assay			IC50 = 0.99291 μM	SANGER
SK-LU-1	Growth Inhibition Assay			IC50 = 0.99868 μM	SANGER
TK10	Growth Inhibition Assay			IC50 = 0.99868 μM	SANGER
8505C	Growth Inhibition Assay			IC50 = 1.03998 μM	SANGER
NCCIT	Growth Inhibition Assay			IC50 = 1.06873 μM	SANGER
NCI-H510A	Growth Inhibition Assay			IC50 = 1.09156 μM	SANGER
NUGC-3	Growth Inhibition Assay			IC50 = 1.13021 μM	SANGER
DJM-1	Growth Inhibition Assay			IC50 = 1.13172 μM	SANGER
MCF7	Growth Inhibition Assay			IC50 = 1.16584 μM	SANGER
GI-1	Growth Inhibition Assay			IC50 = 1.20001 μM	SANGER
NCI-H747	Growth Inhibition Assay			IC50 = 1.21374 μM	SANGER
RPMI-7951	Growth Inhibition Assay			IC50 = 1.24443 μM	SANGER
RCM-1	Growth Inhibition Assay			IC50 = 1.26178 μM	SANGER
CHP-126	Growth Inhibition Assay			IC50 = 1.26806 μM	SANGER
DU-4475	Growth Inhibition Assay			IC50 = 1.26828 μM	SANGER
NCI-H526	Growth Inhibition Assay			IC50 = 1.27996 μM	SANGER
FTC-133	Growth Inhibition Assay			IC50 = 1.29359 μM	SANGER
NOS-1	Growth Inhibition Assay			IC50 = 1.30544 μM	SANGER
HuP-T4	Growth Inhibition Assay			IC50 = 1.3128 μM	SANGER
YT	Growth Inhibition Assay			IC50 = 1.31567 μM	SANGER
SF268	Growth Inhibition Assay			IC50 = 1.31797 μM	SANGER
KYSE-70	Growth Inhibition Assay			IC50 = 1.37884 μM	SANGER
ES8	Growth Inhibition Assay			IC50 = 1.39853 μM	SANGER
MC-CAR	Growth Inhibition Assay			IC50 = 1.39929 μM	SANGER
SW626	Growth Inhibition Assay			IC50 = 1.40825 μM	SANGER
BCPAP	Growth Inhibition Assay			IC50 = 1.43514 μM	SANGER
MRK-nu-1	Growth Inhibition Assay			IC50 = 1.45252 μM	SANGER
SK-PN-DW	Growth Inhibition Assay			IC50 = 1.45278 μM	SANGER
8-MG-BA	Growth Inhibition Assay			IC50 = 1.47953 μM	SANGER
MG-63	Growth Inhibition Assay			IC50 = 1.48966 μM	SANGER
LCLC-97TM1	Growth Inhibition Assay			IC50 = 1.49578 μM	SANGER
MDA-MB-175-VII	Growth Inhibition Assay			IC50 = 1.51005 μM	SANGER
697	Growth Inhibition Assay			IC50 = 1.57595 μM	SANGER
SW837	Growth Inhibition Assay			IC50 = 1.60642 μM	SANGER
ES3	Growth Inhibition Assay			IC50 = 1.60859 μM	SANGER
EHEB	Growth Inhibition Assay			IC50 = 1.60861 μM	SANGER
SNB75	Growth Inhibition Assay			IC50 = 1.60892 μM	SANGER
Daudi	Growth Inhibition Assay			IC50 = 1.67096 μM	SANGER
MES-SA	Growth Inhibition Assay			IC50 = 1.67344 μM	SANGER
LK-2	Growth Inhibition Assay			IC50 = 1.68921 μM	SANGER
647-V	Growth Inhibition Assay			IC50 = 1.73341 μM	SANGER
MOLT-4	Growth Inhibition Assay			IC50 = 1.77334 μM	SANGER
OCUB-M	Growth Inhibition Assay			IC50 = 1.81357 μM	SANGER
EW-1	Growth Inhibition Assay			IC50 = 1.81784 μM	SANGER
BHY	Growth Inhibition Assay			IC50 = 1.82747 μM	SANGER
Raji	Growth Inhibition Assay			IC50 = 1.84743 μM	SANGER
KYSE-510	Growth Inhibition Assay			IC50 = 1.85749 μM	SANGER
KYSE-450	Growth Inhibition Assay			IC50 = 1.93424 μM	SANGER
SW962	Growth Inhibition Assay			IC50 = 1.95452 μM	SANGER
MHH-NB-11	Growth Inhibition Assay			IC50 = 2.00542 μM	SANGER
PA-1	Growth Inhibition Assay			IC50 = 2.01527 μM	SANGER
KURAMOCHI	Growth Inhibition Assay			IC50 = 2.03812 μM	SANGER
SU-DHL-1	Growth Inhibition Assay			IC50 = 2.04945 μM	SANGER
AU565	Growth Inhibition Assay			IC50 = 2.05402 μM	SANGER
MC116	Growth Inhibition Assay			IC50 = 2.11053 μM	SANGER
A431	Growth Inhibition Assay			IC50 = 2.11197 μM	SANGER
OE19	Growth Inhibition Assay			IC50 = 2.14352 μM	SANGER
CAL-33	Growth Inhibition Assay			IC50 = 2.14602 μM	SANGER
KP-4	Growth Inhibition Assay			IC50 = 2.17066 μM	SANGER
SJSA-1	Growth Inhibition Assay			IC50 = 2.18726 μM	SANGER
COLO-800	Growth Inhibition Assay			IC50 = 2.20714 μM	SANGER
NH-12	Growth Inhibition Assay			IC50 = 2.20811 μM	SANGER
IPC-298	Growth Inhibition Assay			IC50 = 2.21227 μM	SANGER
OCI-AML2	Growth Inhibition Assay			IC50 = 2.25537 μM	SANGER
HH	Growth Inhibition Assay			IC50 = 2.28651 μM	SANGER
BFTC-905	Growth Inhibition Assay			IC50 = 2.31888 μM	SANGER
RT-112	Growth Inhibition Assay			IC50 = 2.31957 μM	SANGER
SNU-C2B	Growth Inhibition Assay			IC50 = 2.32484 μM	SANGER
IGR-1	Growth Inhibition Assay			IC50 = 2.34633 μM	SANGER
NCI-H2087	Growth Inhibition Assay			IC50 = 2.34738 μM	SANGER
EoL-1-cell	Growth Inhibition Assay			IC50 = 2.41593 μM	SANGER
SCC-4	Growth Inhibition Assay			IC50 = 2.4302 μM	SANGER
HuH-7	Growth Inhibition Assay			IC50 = 2.43456 μM	SANGER
HSC-2	Growth Inhibition Assay			IC50 = 2.46672 μM	SANGER
NCI-H23	Growth Inhibition Assay			IC50 = 2.46688 μM	SANGER
AN3-CA	Growth Inhibition Assay			IC50 = 2.55034 μM	SANGER
NCI-H2009	Growth Inhibition Assay			IC50 = 2.56438 μM	SANGER
NCI-H1792	Growth Inhibition Assay			IC50 = 2.56683 μM	SANGER
RVH-421	Growth Inhibition Assay			IC50 = 2.57186 μM	SANGER
SK-LMS-1	Growth Inhibition Assay			IC50 = 2.65761 μM	SANGER
COLO-679	Growth Inhibition Assay			IC50 = 2.66172 μM	SANGER
VMRC-RCZ	Growth Inhibition Assay			IC50 = 2.68962 μM	SANGER
NCI-H28	Growth Inhibition Assay			IC50 = 2.73884 μM	SANGER
HT-1197	Growth Inhibition Assay			IC50 = 2.74098 μM	SANGER
OE33	Growth Inhibition Assay			IC50 = 2.75256 μM	SANGER
BT-20	Growth Inhibition Assay			IC50 = 2.767 μM	SANGER
NCI-H661	Growth Inhibition Assay			IC50 = 2.78722 μM	SANGER
HCE-T	Growth Inhibition Assay			IC50 = 2.83188 μM	SANGER
HGC-27	Growth Inhibition Assay			IC50 = 2.83636 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay			IC50 = 2.84084 μM	SANGER
A253	Growth Inhibition Assay			IC50 = 2.85522 μM	SANGER
HCT-116	Growth Inhibition Assay			IC50 = 2.88305 μM	SANGER
CAPAN-1	Growth Inhibition Assay			IC50 = 2.92149 μM	SANGER
MZ2-MEL	Growth Inhibition Assay			IC50 = 2.94674 μM	SANGER
NCI-N87	Growth Inhibition Assay			IC50 = 2.96422 μM	SANGER
NCI-H2052	Growth Inhibition Assay			IC50 = 2.96513 μM	SANGER
DMS-273	Growth Inhibition Assay			IC50 = 2.98076 μM	SANGER
MC-IXC	Growth Inhibition Assay			IC50 = 2.99693 μM	SANGER
OS-RC-2	Growth Inhibition Assay			IC50 = 3.02325 μM	SANGER
TYK-nu	Growth Inhibition Assay			IC50 = 3.03067 μM	SANGER
EW-7	Growth Inhibition Assay			IC50 = 3.05866 μM	SANGER
SK-MES-1	Growth Inhibition Assay			IC50 = 3.07459 μM	SANGER
SF126	Growth Inhibition Assay			IC50 = 3.08914 μM	SANGER
LB647-SCLC	Growth Inhibition Assay			IC50 = 3.14297 μM	SANGER
SCC-3	Growth Inhibition Assay			IC50 = 3.20606 μM	SANGER
TE-15	Growth Inhibition Assay			IC50 = 3.22315 μM	SANGER
M14	Growth Inhibition Assay			IC50 = 3.22456 μM	SANGER
COLO-205	Growth Inhibition Assay			IC50 = 3.24454 μM	SANGER
YKG-1	Growth Inhibition Assay			IC50 = 3.25583 μM	SANGER
SAS	Growth Inhibition Assay			IC50 = 3.26145 μM	SANGER
G-401	Growth Inhibition Assay			IC50 = 3.2899 μM	SANGER
CAL-62	Growth Inhibition Assay			IC50 = 3.34045 μM	SANGER
HCC1599	Growth Inhibition Assay			IC50 = 3.36875 μM	SANGER
JiyoyeP-2003	Growth Inhibition Assay			IC50 = 3.38494 μM	SANGER
IA-LM	Growth Inhibition Assay			IC50 = 3.4017 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay			IC50 = 3.43354 μM	SANGER
SW872	Growth Inhibition Assay			IC50 = 3.45241 μM	SANGER
SW620	Growth Inhibition Assay			IC50 = 3.55738 μM	SANGER
M059J	Growth Inhibition Assay			IC50 = 3.76703 μM	SANGER
KYSE-180	Growth Inhibition Assay			IC50 = 3.79836 μM	SANGER
BxPC-3	Growth Inhibition Assay			IC50 = 3.80576 μM	SANGER
D-283MED	Growth Inhibition Assay			IC50 = 3.81218 μM	SANGER
KYSE-140	Growth Inhibition Assay			IC50 = 3.89603 μM	SANGER
BC-1	Growth Inhibition Assay			IC50 = 3.91812 μM	SANGER
HLE	Growth Inhibition Assay			IC50 = 3.99693 μM	SANGER
MN-60	Growth Inhibition Assay			IC50 = 4.05162 μM	SANGER
NCI-H1304	Growth Inhibition Assay			IC50 = 4.1047 μM	SANGER
IM-9	Growth Inhibition Assay			IC50 = 4.13195 μM	SANGER
NOMO-1	Growth Inhibition Assay			IC50 = 4.20151 μM	SANGER
SW1990	Growth Inhibition Assay			IC50 = 4.24402 μM	SANGER
NCI-H2405	Growth Inhibition Assay			IC50 = 4.27798 μM	SANGER
LAMA-84	Growth Inhibition Assay			IC50 = 4.28656 μM	SANGER
CGTH-W-1	Growth Inhibition Assay			IC50 = 4.30277 μM	SANGER
EFO-21	Growth Inhibition Assay			IC50 = 4.33027 μM	SANGER
U251	Growth Inhibition Assay			IC50 = 4.36314 μM	SANGER
OVCAR-4	Growth Inhibition Assay			IC50 = 4.41035 μM	SANGER
HO-1-N-1	Growth Inhibition Assay			IC50 = 4.41781 μM	SANGER
CaR-1	Growth Inhibition Assay			IC50 = 4.44726 μM	SANGER
CCF-STTG1	Growth Inhibition Assay			IC50 = 4.56209 μM	SANGER
MONO-MAC-6	Growth Inhibition Assay			IC50 = 4.59384 μM	SANGER
CAL-148	Growth Inhibition Assay			IC50 = 4.60119 μM	SANGER
OVCAR-5	Growth Inhibition Assay			IC50 = 4.74899 μM	SANGER
NCI-H460	Growth Inhibition Assay			IC50 = 4.75506 μM	SANGER
HuP-T3	Growth Inhibition Assay			IC50 = 4.79184 μM	SANGER
SK-OV-3	Growth Inhibition Assay			IC50 = 4.88426 μM	SANGER
VM-CUB-1	Growth Inhibition Assay			IC50 = 4.91668 μM	SANGER
CTV-1	Growth Inhibition Assay			IC50 = 4.92002 μM	SANGER
AM-38	Growth Inhibition Assay			IC50 = 4.94112 μM	SANGER
GCT	Growth Inhibition Assay			IC50 = 4.99065 μM	SANGER
NCI-H2347	Growth Inhibition Assay			IC50 = 5.11661 μM	SANGER
DB	Growth Inhibition Assay			IC50 = 5.11905 μM	SANGER
FADU	Growth Inhibition Assay			IC50 = 5.13773 μM	SANGER
Calu-1	Growth Inhibition Assay			IC50 = 5.13779 μM	SANGER
NCI-H2030	Growth Inhibition Assay			IC50 = 5.21763 μM	SANGER
DOHH-2	Growth Inhibition Assay			IC50 = 5.28851 μM	SANGER
NCI-H1770	Growth Inhibition Assay			IC50 = 5.29092 μM	SANGER
P12-ICHIKAWA	Growth Inhibition Assay			IC50 = 5.54575 μM	SANGER
HT	Growth Inhibition Assay			IC50 = 5.55505 μM	SANGER
CAL-120	Growth Inhibition Assay			IC50 = 5.56142 μM	SANGER
DBTRG-05MG	Growth Inhibition Assay			IC50 = 5.67305 μM	SANGER
COLO-680N	Growth Inhibition Assay			IC50 = 5.7583 μM	SANGER
HT-3	Growth Inhibition Assay			IC50 = 5.77714 μM	SANGER
NY	Growth Inhibition Assay			IC50 = 5.91176 μM	SANGER
NCI-H838	Growth Inhibition Assay			IC50 = 5.94757 μM	SANGER
NCI-H1618	Growth Inhibition Assay			IC50 = 5.98557 μM	SANGER
TE-11	Growth Inhibition Assay			IC50 = 6.08459 μM	SANGER
Calu-3	Growth Inhibition Assay			IC50 = 6.19074 μM	SANGER
ST486	Growth Inhibition Assay			IC50 = 6.39405 μM	SANGER
EW-11	Growth Inhibition Assay			IC50 = 6.41293 μM	SANGER
TE-1	Growth Inhibition Assay			IC50 = 6.42507 μM	SANGER
NB5	Growth Inhibition Assay			IC50 = 6.72078 μM	SANGER
DMS-114	Growth Inhibition Assay			IC50 = 6.78617 μM	SANGER
KYSE-520	Growth Inhibition Assay			IC50 = 6.78887 μM	SANGER
SIMA	Growth Inhibition Assay			IC50 = 6.93011 μM	SANGER
KM-H2	Growth Inhibition Assay			IC50 = 6.96614 μM	SANGER
WM-115	Growth Inhibition Assay			IC50 = 7.09344 μM	SANGER
P30-OHK	Growth Inhibition Assay			IC50 = 7.09585 μM	SANGER
22RV1	Growth Inhibition Assay			IC50 = 7.32932 μM	SANGER
BHT-101	Growth Inhibition Assay			IC50 = 7.34054 μM	SANGER
COR-L105	Growth Inhibition Assay			IC50 = 7.36534 μM	SANGER
Calu-6	Growth Inhibition Assay			IC50 = 7.67159 μM	SANGER
C8166	Growth Inhibition Assay			IC50 = 7.77036 μM	SANGER
SH-4	Growth Inhibition Assay			IC50 = 7.84755 μM	SANGER
RPMI-8866	Growth Inhibition Assay			IC50 = 7.9095 μM	SANGER
TE-5	Growth Inhibition Assay			IC50 = 7.96408 μM	SANGER
CAL-27	Growth Inhibition Assay			IC50 = 8.07296 μM	SANGER
KYSE-410	Growth Inhibition Assay			IC50 = 8.1465 μM	SANGER
NCI-H1355	Growth Inhibition Assay			IC50 = 8.20414 μM	SANGER
LB831-BLC	Growth Inhibition Assay			IC50 = 8.38272 μM	SANGER
SK-MEL-2	Growth Inhibition Assay			IC50 = 8.48548 μM	SANGER
JEG-3	Growth Inhibition Assay			IC50 = 8.5506 μM	SANGER
NCI-H441	Growth Inhibition Assay			IC50 = 9.04985 μM	SANGER
KM12	Growth Inhibition Assay			IC50 = 9.18802 μM	SANGER
OAW-28	Growth Inhibition Assay			IC50 = 9.20646 μM	SANGER
HOP-92	Growth Inhibition Assay			IC50 = 9.26 μM	SANGER
HT-1376	Growth Inhibition Assay			IC50 = 9.73755 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay			IC50 = 9.88105 μM	SANGER
NCI-H727	Growth Inhibition Assay			IC50 = 10.3102 μM	SANGER
A673	Growth Inhibition Assay			IC50 = 10.4566 μM	SANGER
A704	Growth Inhibition Assay			IC50 = 10.6436 μM	SANGER
HEL	Growth Inhibition Assay			IC50 = 10.8362 μM	SANGER
HuCCT1	Growth Inhibition Assay			IC50 = 10.9343 μM	SANGER
U-87-MG	Growth Inhibition Assay			IC50 = 11.0572 μM	SANGER
LC4-1	Growth Inhibition Assay			IC50 = 11.1604 μM	SANGER
SW756	Growth Inhibition Assay			IC50 = 11.3757 μM	SANGER
NCI-H1882	Growth Inhibition Assay			IC50 = 11.455 μM	SANGER
NB1	Growth Inhibition Assay			IC50 = 11.5965 μM	SANGER
KALS-1	Growth Inhibition Assay			IC50 = 11.7421 μM	SANGER
OMC-1	Growth Inhibition Assay			IC50 = 11.8941 μM	SANGER
Detroit562	Growth Inhibition Assay			IC50 = 11.931 μM	SANGER
PANC-08-13	Growth Inhibition Assay			IC50 = 12.0521 μM	SANGER
TE-6	Growth Inhibition Assay			IC50 = 12.3857 μM	SANGER
GCIY	Growth Inhibition Assay			IC50 = 12.7546 μM	SANGER
U-698-M	Growth Inhibition Assay			IC50 = 13.0065 μM	SANGER
PC-3	Growth Inhibition Assay			IC50 = 13.3146 μM	SANGER
T84	Growth Inhibition Assay			IC50 = 13.4592 μM	SANGER
NCI-H596	Growth Inhibition Assay			IC50 = 13.5755 μM	SANGER
D-392MG	Growth Inhibition Assay			IC50 = 13.5799 μM	SANGER
NB10	Growth Inhibition Assay			IC50 = 13.8464 μM	SANGER
BL-41	Growth Inhibition Assay			IC50 = 14.1975 μM	SANGER
HDLM-2	Growth Inhibition Assay			IC50 = 14.3177 μM	SANGER
LAN-6	Growth Inhibition Assay			IC50 = 14.6341 μM	SANGER
CMK	Growth Inhibition Assay			IC50 = 14.7963 μM	SANGER
MDA-MB-453	Growth Inhibition Assay			IC50 = 15.4155 μM	SANGER
GP5d	Growth Inhibition Assay			IC50 = 15.636 μM	SANGER
TCCSUP	Growth Inhibition Assay			IC50 = 15.9419 μM	SANGER
NCI-H520	Growth Inhibition Assay			IC50 = 16.0521 μM	SANGER
ChaGo-K-1	Growth Inhibition Assay			IC50 = 16.3642 μM	SANGER
COLO-741	Growth Inhibition Assay			IC50 = 16.4227 μM	SANGER
NCI-H1693	Growth Inhibition Assay			IC50 = 16.565 μM	SANGER
DG-75	Growth Inhibition Assay			IC50 = 16.9142 μM	SANGER
CAMA-1	Growth Inhibition Assay			IC50 = 17.5247 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay			IC50 = 18.3155 μM	SANGER
EW-3	Growth Inhibition Assay			IC50 = 18.5014 μM	SANGER
TUR	Growth Inhibition Assay			IC50 = 18.8518 μM	SANGER
NCI-H446	Growth Inhibition Assay			IC50 = 18.8813 μM	SANGER
ATN-1	Growth Inhibition Assay			IC50 = 18.9202 μM	SANGER
LU-65	Growth Inhibition Assay			IC50 = 19.4673 μM	SANGER
ABC-1	Growth Inhibition Assay			IC50 = 19.6679 μM	SANGER
NCI-H1648	Growth Inhibition Assay			IC50 = 20.3205 μM	SANGER
Caov-3	Growth Inhibition Assay			IC50 = 20.4613 μM	SANGER
NCI-H1563	Growth Inhibition Assay			IC50 = 20.6061 μM	SANGER
KLE	Growth Inhibition Assay			IC50 = 21.24 μM	SANGER
MMAC-SF	Growth Inhibition Assay			IC50 = 21.327 μM	SANGER
BB65-RCC	Growth Inhibition Assay			IC50 = 21.4262 μM	SANGER
EB-3	Growth Inhibition Assay			IC50 = 21.8331 μM	SANGER
SK-MEL-3	Growth Inhibition Assay			IC50 = 22.1755 μM	SANGER
NCI-H2196	Growth Inhibition Assay			IC50 = 22.8494 μM	SANGER
GR-ST	Growth Inhibition Assay			IC50 = 23.0977 μM	SANGER
SK-MM-2	Growth Inhibition Assay			IC50 = 23.239 μM	SANGER
SK-MEL-24	Growth Inhibition Assay			IC50 = 23.2952 μM	SANGER
LS-123	Growth Inhibition Assay			IC50 = 23.4283 μM	SANGER
SW1463	Growth Inhibition Assay			IC50 = 23.4421 μM	SANGER
L-540	Growth Inhibition Assay			IC50 = 23.5493 μM	SANGER
Capan-2	Growth Inhibition Assay			IC50 = 24.2155 μM	SANGER
NCI-H1838	Growth Inhibition Assay			IC50 = 24.5232 μM	SANGER
TE-441-T	Growth Inhibition Assay			IC50 = 24.6153 μM	SANGER
A4-Fuk	Growth Inhibition Assay			IC50 = 25.288 μM	SANGER
COR-L279	Growth Inhibition Assay			IC50 = 25.3526 μM	SANGER
KE-37	Growth Inhibition Assay			IC50 = 25.9179 μM	SANGER
NCI-H1755	Growth Inhibition Assay			IC50 = 26.001 μM	SANGER
KP-N-YN	Growth Inhibition Assay			IC50 = 26.337 μM	SANGER
COLO-792	Growth Inhibition Assay			IC50 = 26.4052 μM	SANGER
Becker	Growth Inhibition Assay			IC50 = 26.9253 μM	SANGER
no-10	Growth Inhibition Assay			IC50 = 27.0943 μM	SANGER
NCI-H1793	Growth Inhibition Assay			IC50 = 27.172 μM	SANGER
HCC1143	Growth Inhibition Assay			IC50 = 27.1918 μM	SANGER
KARPAS-299	Growth Inhibition Assay			IC50 = 27.2024 μM	SANGER
HEC-1	Growth Inhibition Assay			IC50 = 27.3627 μM	SANGER
SCC-15	Growth Inhibition Assay			IC50 = 27.6315 μM	SANGER
LS-513	Growth Inhibition Assay			IC50 = 28.9662 μM	SANGER
HN	Growth Inhibition Assay			IC50 = 29.1374 μM	SANGER
MOLT-16	Growth Inhibition Assay			IC50 = 29.5165 μM	SANGER
KP-N-YS	Growth Inhibition Assay			IC50 = 30.2856 μM	SANGER
NCI-H1417	Growth Inhibition Assay			IC50 = 30.3916 μM	SANGER
RPMI-8402	Growth Inhibition Assay			IC50 = 30.7398 μM	SANGER
WSU-NHL	Growth Inhibition Assay			IC50 = 31.634 μM	SANGER
SK-NEP-1	Growth Inhibition Assay			IC50 = 31.88 μM	SANGER
HCC1187	Growth Inhibition Assay			IC50 = 32.9082 μM	SANGER
PFSK-1	Growth Inhibition Assay			IC50 = 33.4435 μM	SANGER
TE-9	Growth Inhibition Assay			IC50 = 33.9766 μM	SANGER
NCI-H209	Growth Inhibition Assay			IC50 = 34.3161 μM	SANGER
HL-60	Growth Inhibition Assay			IC50 = 35.2849 μM	SANGER
LS-1034	Growth Inhibition Assay			IC50 = 35.3757 μM	SANGER
NCI-H2141	Growth Inhibition Assay			IC50 = 36.1155 μM	SANGER
COLO-678	Growth Inhibition Assay			IC50 = 36.2389 μM	SANGER
NB13	Growth Inhibition Assay			IC50 = 36.744 μM	SANGER
COLO-829	Growth Inhibition Assay			IC50 = 36.9718 μM	SANGER
BB49-HNC	Growth Inhibition Assay			IC50 = 38.7612 μM	SANGER
NCI-H1623	Growth Inhibition Assay			IC50 = 39.0477 μM	SANGER
A172	Growth Inhibition Assay			IC50 = 39.7268 μM	SANGER
MPP-89	Growth Inhibition Assay			IC50 = 39.8805 μM	SANGER
ME-180	Growth Inhibition Assay			IC50 = 40.8628 μM	SANGER
LU-165	Growth Inhibition Assay			IC50 = 41.7959 μM	SANGER
LU-134-A	Growth Inhibition Assay			IC50 = 41.8216 μM	SANGER
HCC2218	Growth Inhibition Assay			IC50 = 41.8898 μM	SANGER
NCI-H1694	Growth Inhibition Assay			IC50 = 43.8822 μM	SANGER
BEN	Growth Inhibition Assay			IC50 = 46.0431 μM	SANGER
RERF-LC-MS	Growth Inhibition Assay			IC50 = 48.6853 μM	SANGER
LC-1F	Growth Inhibition Assay			IC50 = 49.4076 μM	SANGER
L-428	Growth Inhibition Assay			IC50 = 52.3358 μM	SANGER
CPC-N	Growth Inhibition Assay			IC50 = 52.489 μM	SANGER
COLO-824	Growth Inhibition Assay			IC50 = 53.329 μM	SANGER
IST-SL1	Growth Inhibition Assay			IC50 = 54.5851 μM	SANGER
NCI-H810	Growth Inhibition Assay			IC50 = 55.1277 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay			IC50 = 56.4075 μM	SANGER
ECC4	Growth Inhibition Assay			IC50 = 56.7007 μM	SANGER
TT	Growth Inhibition Assay			IC50 = 59.1501 μM	SANGER
D-542MG	Growth Inhibition Assay			IC50 = 60.2794 μM	SANGER
T98G	Growth Inhibition Assay			IC50 = 63.7348 μM	SANGER
U-118-MG	Growth Inhibition Assay			IC50 = 65.6647 μM	SANGER
TGW	Growth Inhibition Assay			IC50 = 65.7504 μM	SANGER
MKN7	Growth Inhibition Assay			IC50 = 66.5296 μM	SANGER
MDA-MB-415	Growth Inhibition Assay			IC50 = 67.3338 μM	SANGER
SNU-475	Growth Inhibition Assay			IC50 = 67.5008 μM	SANGER
MDA-MB-468	Growth Inhibition Assay			IC50 = 67.5522 μM	SANGER
KU812	Growth Inhibition Assay			IC50 = 67.9601 μM	SANGER
RCC10RGB	Growth Inhibition Assay			IC50 = 70.5644 μM	SANGER
K5	Growth Inhibition Assay			IC50 = 72.3646 μM	SANGER
NCI-H1155	Growth Inhibition Assay			IC50 = 73.2129 μM	SANGER
COR-L88	Growth Inhibition Assay			IC50 = 73.4243 μM	SANGER
TGBC1TKB	Growth Inhibition Assay			IC50 = 74.14 μM	SANGER
NCI-H748	Growth Inhibition Assay			IC50 = 75.1768 μM	SANGER
EW-18	Growth Inhibition Assay			IC50 = 75.8498 μM	SANGER
NCI-H226	Growth Inhibition Assay			IC50 = 77.3602 μM	SANGER
HTC-C3	Growth Inhibition Assay			IC50 = 77.8428 μM	SANGER
CAS-1	Growth Inhibition Assay			IC50 = 81.0296 μM	SANGER
SNU-C1	Growth Inhibition Assay			IC50 = 81.9941 μM	SANGER
NB12	Growth Inhibition Assay			IC50 = 82.3997 μM	SANGER
AsPC-1	Growth Inhibition Assay			IC50 = 82.5028 μM	SANGER
HCC1954	Growth Inhibition Assay			IC50 = 84.163 μM	SANGER
EM-2	Growth Inhibition Assay			IC50 = 84.7528 μM	SANGER
CA46	Growth Inhibition Assay			IC50 = 84.8498 μM	SANGER
no-11	Growth Inhibition Assay			IC50 = 85.5708 μM	SANGER
DK-MG	Growth Inhibition Assay			IC50 = 85.7672 μM	SANGER
HuO-3N1	Growth Inhibition Assay			IC50 = 87.8298 μM	SANGER
S-117	Growth Inhibition Assay			IC50 = 90.2444 μM	SANGER
C2BBe1	Growth Inhibition Assay			IC50 = 91.8016 μM	SANGER
THP-1	Growth Inhibition Assay			IC50 = 92.4868 μM	SANGER
HCC2157	Growth Inhibition Assay			IC50 = 93.3592 μM	SANGER
EW-24	Growth Inhibition Assay			IC50 = 95.3316 μM	SANGER
UACC-812	Growth Inhibition Assay			IC50 = 99.5951 μM	SANGER
NCI-H720	Growth Inhibition Assay			IC50 = 103.351 μM	SANGER
NCI-H69	Growth Inhibition Assay			IC50 = 103.909 μM	SANGER
OPM-2	Growth Inhibition Assay			IC50 = 104.066 μM	SANGER
RD	Growth Inhibition Assay			IC50 = 105.698 μM	SANGER
NCI-H1581	Growth Inhibition Assay			IC50 = 110.224 μM	SANGER
OVCAR-3	Growth Inhibition Assay			IC50 = 123.332 μM	SANGER
HCC70	Growth Inhibition Assay			IC50 = 125.868 μM	SANGER
GAK	Growth Inhibition Assay			IC50 = 126.132 μM	SANGER
K-562	Growth Inhibition Assay			IC50 = 126.227 μM	SANGER
UACC-893	Growth Inhibition Assay			IC50 = 129.777 μM	SANGER
SKG-IIIa	Growth Inhibition Assay			IC50 = 136.469 μM	SANGER
SHP-77	Growth Inhibition Assay			IC50 = 142.135 μM	SANGER
D-263MG	Growth Inhibition Assay			IC50 = 142.206 μM	SANGER
Mewo	Growth Inhibition Assay			IC50 = 147.962 μM	SANGER
MSTO-211H	Growth Inhibition Assay			IC50 = 149.459 μM	SANGER
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (66.11 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (1.65mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	1512.62	Formula	C₅₅H₈₅N₁₇O₂₅S₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	9041-93-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC125066 sulfate			Smiles	CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)OC3C(C(C(C(O3)CO)O)O)OC4C(C(C(C(O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-]

Mechanism of Action

In vitro	UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL this compound for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without this chemical. Macrophages stimulated with this compound continues to produce significant amounts of fibroblast growth factor even after this chemical is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by this compound-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. This chemical (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. In terms of unstable aberrations, the clastogenic effect of this compound on ADIPO-P2 cells persists for at least 10 days after exposure. This chemical-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in this compound-exposed cells 10 days after treatment suggests that this chemical can induce delayed telomere instability.
In vivo	Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21.
References	[1] https://pubmed.ncbi.nlm.nih.gov/9288321/ [2] https://pubmed.ncbi.nlm.nih.gov/2464942/ [3] https://pubmed.ncbi.nlm.nih.gov/22469952/ [4] https://pubmed.ncbi.nlm.nih.gov/22564429/ [5] https://pubmed.ncbi.nlm.nih.gov/22643035/ [6] https://pubmed.ncbi.nlm.nih.gov/22640881/ [7] https://pubmed.ncbi.nlm.nih.gov/25356121/ [8] https://pubmed.ncbi.nlm.nih.gov/27330564/

Applications

Methods	Biomarkers	PMID
Western blot	LRP6 / Wnt5AB / Dvl2 / β-catenin phospho-eIF2α / eIF2α cytochrome c Bcl-xl Bcl-2	27649046
Immunofluorescence	LC3	23762310
ELISA	HMGB1	23762310

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

Question 1:
What is the ratio between bleomycin A2 and bleomycin B2 in it?

Answer:
Its content of Bleomycin A2 and Bleomycin B2 is 62% and 29% respectively.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):