Bleomycin (NSC125066) sulfate

For research use only.

Catalog No.S1214

57 publications

Bleomycin (NSC125066) sulfate Chemical Structure

CAS No. 9041-93-4

Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.

Size Price Stock Quantity  
10mM (1mL in DMSO) RMB 3415.58 In stock
RMB 1630.13 In stock
RMB 8108.21 In stock

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Selleck's Bleomycin (NSC125066) sulfate has been cited by 57 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.
In vitro

UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. [1] Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL Bleomycin sulfate for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without Bleomycin sulfate. Macrophages stimulated with Bleomycin sulfate continues to produce significant amounts of fibroblast growth factor even after Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by Bleomycin sulfate-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. [2] Bleomycin sulfate (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. [3] In terms of unstable aberrations, the clastogenic effect of Bleomycin sulfate on ADIPO-P2 cells persists for at least 10 days after exposure. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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POLK KO NFr4W2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;WUWlEPTB;ND6wN-KBkcLz4pEJNU46OsLizszN MnWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyM{G0NFAoRjJ4MEOxOFAxRC:jPh?=
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A431 NVTmfGViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLEOVAuOjByIFnV MojaOFjjiImq MnvQbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyMUK5PUc,OjVzMEGyPVk9N2F-
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NCCIT NY\kUIo{TnWwY4Tpc44hSXO|YYm= MnvWNVIxKM7:Zz;tcC=> M1Hz[|czKGh? Mn3Ed4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiUILveIVqdiClYYLic456dCCuZY\lcJM> NXL3N3VpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NTera-2 M1i3NGZ2dmO2aX;uJGF{e2G7 NVzoOJhEOTJyIN88[{9udA>? M2\yOVczKGh? NXK2cpk4e2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgUHBQKGyndnXsdy=> MknIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MkWzOVUoRjJ{OEK1N|U2RC:jPh?=
NCCIT M{L5OWZ2dmO2aX;uJGF{e2G7 M3rrRVEzOCEQvHevcYw> MoXzO|IhcA>? M{S3dpNq\26rZnnjZY51dHliaX7jdoVie2W|IFzQU{Bt\X[nbIO= NFK0dGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVM2PSd-MkK4NlU{PTV:L3G+
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NCCIT  M3jidWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGf5U2szOOLCk{G0NOKh|rypL33s M3L1UFczKGh? Mn;qUGQ2OD1zMkFCpO69\y:vbNMg NHnV[Yk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW2NlE3OCd-MkK1OlIyPjB:L3G+
NCCIT  NEe5cWFHfW6ldHnvckBCe3OjeR?= NGjQ[Ig4OiCq MX7zbYdvcW[rY3HueIx6KGmwY4LlZZNmeyClYYPwZZNmNTNiYXP0bZZqfHoEoB?= Mlf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkKxOlAoRjJ{NU[yNVYxRC:jPh?=
NCCIT  MnfjSpVv[3Srb36gRZN{[Xl? NVXseXE{PzJiaB?= NH7wcZR{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDjZZNx[XOnLUigZYN1cX[rdIpCpC=> Mk\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkKxOlAoRjJ{NU[yNVYxRC:jPh?=
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MCF-7  MnyySpVv[3Srb36gRZN{[Xl? NWPTVlRLOzBxN{Cg{txoN22u NXzycpNrOjUEoHi= MoPqbY5kemWjc3XzJJRp\SCvUl7BJIxmfmWuczDv[uKheDV|78{MRXRONHB{MdMgZY5lyqCJQVTEOFU> NFfWclg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4O|k{Pyd-MkK0PFc6Ozd:L3G+
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MCF-7  M3HwbWN6fG:2b4jpZ4l1gSCDc4PhfS=> MmLPN|AwPzBizsznM41t MkH0NlTDqGh? MX\pcoR2[2W|IHjp[4htgSC|aXfubYZq[2GwdDDs[ZZmdHNib3[g[pJi\22nboTl[EBFVkFicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6N{mzO{c,OjJ2OEe5N|c9N2F-
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HCC1937 MkjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxKD1iMD6wNFAzPTRizszN NHrHbnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
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NCI-H720 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7sV4tKSzVyIE2gNVA{NjN3MTFOwG0> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
NCI-H69 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mne4TWM2OCB;IEGwN{46ODlizszN NHvoWmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
OPM-2 M1Pyd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1T5eGlEPTBiPTCxNFQvODZ4IN88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
RD MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXNTWM2OCB;IEGwOU43QThizszN MmTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
NCI-H1581 Mme2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHlTWM2OCB;IEGxNE4zOjRizszN M4LaWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
OVCAR-3 M3;0Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxKD1iMUKzMlM{OiEQvF2= Mlz2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
HCC70 NGf6[mpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxKD1iMUK1Mlg3QCEQvF2= MonXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
GAK M1:zW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXL5enVrUUN3MDC9JFEzPi5zM{Kg{txO MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
K-562 NW\ofHE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxKD1iMUK2MlIzPyEQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
UACC-893 NH3QVY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxKD1iMUK5Mlc4PyEQvF2= M3PKclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
SKG-IIIa MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxKD1iMUO2MlQ3QSEQvF2= NVXtfpRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
SHP-77 M4O1Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULkWZU3UUN3MDC9JFE1Oi5zM{Wg{txO M1\HTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
D-263MG M3PNPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYr1Zos6UUN3MDC9JFE1Oi5{ME[g{txO NEDDeWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
Mewo MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELSNnZKSzVyIE2gNVQ4Njl4MjFOwG0> MmHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
MSTO-211H MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxKD1iMUS5MlQ2QSEQvF2= NVG2SVRJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LRP6 / Wnt5AB / Dvl2 / β-catenin; 

PubMed: 27649046     


CRL-1490 cells were treated with the indicated concentrations of bleomycin for either 12 or 24 h, and then probed for canonical Wnt proteins LRP6, Wnt5AB, Dvl2, and β-catenin. 

phospho-eIF2α / eIF2α; 

PubMed: 23762310     


Western blotting analysis of phosphorylated, total eIF2α and β-actin in CT26 treated 24 h with the indicated drugs. BLM: Bleomycin

cytochrome c; 

PubMed: 20154224     


mitochondrial release of cytochrome c was detected by Western blot in cytosolic extract and analyzed by band densitometry. Data show the means of four independent experiments ± SE. *Statistical significance from control, P < 0.05. Representative Western blots are shown.

Bcl-xl; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-xL.

Bcl-2; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-2.

27649046 23762310 20154224
Immunofluorescence
LC3; 

PubMed: 23762310     


CT26 cells were cultured on glass slides and treated with indicated drugs for 24 h. The cells were then submitted to anti-LC3 (green) and DAPI (blue) labeling and observed under an epifluorescence microscope. BLM: Bleomycin

23762310
ELISA
HMGB1; 

PubMed: 23762310     


HMGB1 was titrated with ELISA method in supernatant of CT26 cells treated 24 h with indicated drugs. BLM: Bleomycin

23762310
In vivo Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21. [5]

Protocol

Cell Research:

[4]

- Collapse
  • Cell lines: ADIPO-P2 cells
  • Concentrations: 2.5 μg/mL
  • Incubation Time: 30 minutes
  • Method:

    ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH. Two independent experiments are carried out.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: CD-1 mice
  • Dosages: 5 mg/kg, 2 ml/kg
  • Administration: Administered via i.t.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (66.11 mM)
Water 100 mg/mL (66.11 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1512.62
Formula

C55H85N17O25S4

CAS No. 9041-93-4
Storage powder
in solvent
Synonyms N/A
Smiles CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)OC3C(C(C(C(O3)CO)O)O)OC4C(C(C(C(O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-]

In vivo Formulation Calculator (Clear solution)

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    what is the ratio between bleomycin A2 and bleomycin B2 in your product?

  • Answer:

    The content of Bleomycin A2 and Bleomycin B2 is 62% and 29% respectively.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID