Bleomycin Sulfate

For research use only.

Catalog No.S1214 Synonyms: NSC125066

38 publications

Bleomycin Sulfate Chemical Structure

CAS No. 9041-93-4

Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.

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Selleck's Bleomycin Sulfate has been cited by 38 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.
In vitro

UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. [1] Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL Bleomycin sulfate for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without Bleomycin sulfate. Macrophages stimulated with Bleomycin sulfate continues to produce significant amounts of fibroblast growth factor even after Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by Bleomycin sulfate-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. [2] Bleomycin sulfate (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. [3] In terms of unstable aberrations, the clastogenic effect of Bleomycin sulfate on ADIPO-P2 cells persists for at least 10 days after exposure. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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POLK WT Mli0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTFzLkhihKnDueLCiUGuO:Kh|ryP NVnvcHlwOjZyM{G0NFA>
POLK KO Mlj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXsbGRjUUN3ME20MlA{6oDLwsJihKkyNjl{wrFOwG0> MnzoNlYxOzF2MEC=
POLK CD NG\QV2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;tNHlKSzVyPUWuOVnjiIoEsfMAjVEvODEEoN88US=> NWHGSYc1OjZyM{G0NFA>
RLE/Abca3 M{DRS2Z2dmO2aX;uJGF{e2G7 M{W5ZlAvPMLizszN M2nSd|E1PMLiaB?= M{PZU4lv\HWlZYOgZUBud3KyaH;sc4dq[2GuIHPoZY5o\SCocn;tJIVxcXSqZXzpZYwudGmtZTDtc5JxcG:ub3f5JJRwKGFid3nk[Uwhe3C{ZXHkMY92fCCvb4LwbI9td2e7wrC= MnS0NlU4PjB3M{i=
PMCs M4DHXmZ2dmO2aX;uJGF{e2G7 NWLVfItROC5z4pETNuKh|rypL33s M4jOSVI1yqCq NFnEdpRk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcoNz\WG|ZTDpckB1cGVicILveIVqdiCuZY\lcEBw\iClb3zsZYdmdi2LwrC= MVuyOVU6PTZ2Mh?=
PMCs NVHHfVg2TnWwY4Tpc44hSXO|YYm= NXz6bGNWOC5{wrFOwIcwdWx? MUKyOQKBmzd{wrDo M1yxOIlv\HWlZYOgbY5kemWjc3XzJIlvKH[rbXXueIlvKGGwZDFOtU1UVUFicILveIVqdg>? NYTSZmRwOjV3OUW2OFI>
PMCs NIn2VIFHfW6ldHnvckBCe3OjeR?= NYi3OpNmOC5{wrFOwIcwdWx? NEi3Xmk4OiCq MWHpcoR2[2W|IHTlZ5Jm[XOnczDpckBkgXSxa3XyZZRqdi16IHHu[EBGNWOjZHjldolvKHC{b4TlbY4> MUOyOVU6PTZ2Mh?=
PMCs MXrGeY5kfGmxbjDBd5NigQ>? NGHlVZoxNjMEoN88[{9udA>? MnXUNVIwOjRxNEigbC=> MUTpcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? NInVNGkzPTV7NU[0Ni=>
IMR-32 Mn3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGS1WJc4NjYkiKKxNlAhyrWpL33s NXrQeIt6OTMkiKK0PEBp NWGxUldMUUN3ME22NEDDvWdxbXygZZQhfGinIHXu[EBw\iB{NDDodpMhd2ZiaX7jeYJifGmxbh?= MofRNlU2OzZ|NEW=
HT1080 MmTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHrNUDPxE1? MX2yOEBpyqB? M4PVSIxm[WS|IITvJIEhTzJxTTDk[YxigQ>? NV62UmJQOjV3MUi5OlE>
HT1080 MVnGeY5kfGmxbjDBd5NigQ>? MYexJO69VQ>? MnjNNlQhcMLi MUPpcoR2[2W|IHGgdI91\W62IHfsc4JidCCGTlGg[IFu[WenIILld5BwdnOn MnnmNlU2OTh7NkG=
HDFn MXvDfZRwfG:6aXPpeJkhSXO|YYm= NXnsN21vPzJiaB?= Mk\YTWM2OD17LkOxKeKh NHXuZlMzPTJ5Nke5Ni=>
THP-1 NEfoeFhEgXSxdH;4bYNqfHliQYPzZZk> MW[3NkBp MUnJR|UxRTRwN{el M2PvRlI2Ojd4N{my
HT-29 NIPJNm1EgXSxdH;4bYNqfHliQYPzZZk> NHXhSlI4OiCq M1rpdGlEPTB;MUGuOFkm NEf6PYYzPTJ5Nke5Ni=>
HCT116  NGraSpZEgXSxdH;4bYNqfHliQYPzZZk> M3i1VFczKGh? NHvXUHVKSzVyPUGxMlM1LQ>? M2LmSFI2Ojd4N{my
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T24 MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3ZOVAuOjByIFnV NYnhcopiPDkkgJno MlXMbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> NUC2Xo1rOjVzMEGyPVk>
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A549 MVvGeY5kfGmxbjDBd5NigQ>? M1nYXFEhyrWpL33MxsA> M3zhRVAuPDhiaB?= NEXQcoZqdmO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4bDqE:STtMgcXJPSQ>? NH3zeGMzPDl4M{[zOS=>
MLE12 MYjGeY5kfGmxbjDBd5NigQ>? NUnwOW1POSEEtXevcWzDqA>? MlL1NE01QCCq MV\pcoNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3dCpG9RVsLibWLORS=> NH:0VZozPDl4M{[zOS=>
Jurkat NUnRXldST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVKyNQKBkc7:bR?= NHPwOmIzPOLCiXlCpC=> NG\3fmxienKnc4TzJJRp\SClZXzsJIN6[2ynIHH0JJRp\SCJMjDwbIF{\Q>? M{T4TVI1QTF4OEmz
HeLa  M4P0U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFL3WmpKSzVyPUGwMlLDqM7:TR?= NU[5Wm91OjR5M{KzPVc>
C18-4 NHLLeY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXKwMVExOCEQvF2= NUfKN5BLcW6qaXLpeJMh[2WubDDndo94fGhiZH;z[UBl\XCnbnTlcpRtgQ>? Mlf0NlQ2PzF7OEK=
BMG-1  MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVy0NE85OMLizsznM40> NFL3ZWM{yqCq M2\yc4lvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> MmWwNlM6ODZ5Mk[=
A459 Ml3DRZBweHSxc3nzJGF{e2G7 MkjFNVAhdVV? NXHvUFRqPDhiaB?= MmHtbY5lfWOnczDhdI9xfG:|aYO= MUWyN|kxOjd4Nh?=
MOCK NYnjOXpySXCxcITvd4l{KEG|c3H5 MlyxNVAhdVV? MX:0PEBp MorFbY5lfWOnczDhdI9xfG:|aYO= NEHMZWUzOzlyMke2Oi=>
MMP1 MofaRZBweHSxc3nzJGF{e2G7 NVv0eGhKOTBibWW= NIO4WGM1QCCq M3XneIlv\HWlZYOgZZBweHSxc3nz MUmyN|kxOjd4Nh?=
A549 MmS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvhUW1[OjByIN88US=> NE\Lbm8zPCCqwrC= MlTm[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> Ml7VNlM5ODV5OUO=
HCT-116 NETXc3VCeG:ydH;zbZMhSXO|YYm= M12ySlMwPy53L{G1JO69\y:vbB?= MnfKOFghcA>? NVu5WZR6emWmdXPld{B1cGVibHX2[Ywhd2ZiQlzIJIF1KGirZ3igZ49v[2WwdILheIlwdg>? Ml24NlM4ODh4Nki=
HeLa NWnnWoh3SXCxcITvd4l{KEG|c3H5 NUTYUJhrOy95LkWvNVUh|rypL33s NWT5fWFlPDhiaB?= NIrwXnBz\WS3Y3XzJJRp\SCuZY\lcEBw\iCETFigZZQhcGmpaDDjc45k\W62cnH0bY9v NYDBd2dPOjN5MEi2Olg>
HCT116 NF;HV2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjx[oFUOC1zMECg{txoN22u NVTJOnhqPDhiaB?= M3LDdIlvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> NYrrdINwOjN3MUiyNFE>
NCM460 MmPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVywMVExOCEQvHevcYw> NUDZSpJWPDhiaB?= MkfCbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> M{P6eVI{PTF6MkCx
NT2  NUnrVWxTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;i[HBmOjRiaNMg MnLuUGQ2OD12MEFihKnDvWdxbXy= MXyyN|M5PjR{MB?=
NT2  NI\UfYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPtPIxmPDhiaB?= MVLMSFUxRTFyMDFCuYcwdWx? NIPhR|gzOzN6NkSyNC=>
NT2  M1\JXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLkR4g4OiCq MlLQUGQ2OD1{MPMAjeK2\y:vbB?= MVeyN|M5PjR{MB?=
NT2  M12zV2Fxd3C2b4Ppd{BCe3OjeR?= Ml3NOFAx6oDLwsXnM41t MU[yOEBpyqB? M{TXfYlv\HWlZYOgd4lodmmoaXPhcpQhcW6lcnXhd4V{KGmwIHPhd5Bie2VvMzy4MFkh[WO2aY\peJnDqA>? MVuyN|M5PjR{MB?=
NTera-2 NUnlNpY2TnWwY4Tpc44hSXO|YYm= NFfQWogyOjBizsznM41t MVe3NkBp MXrzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyB6LXnzc5Bzd3O2YX7lJIxmfmWucx?= NIWxNIkzOjh{NUO1OS=>
NCCIT MWPGeY5kfGmxbjDBd5NigQ>? NGTucmkyOjBizsznM41t MX23NkBp NEXWb|l{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczC4MYl{d3C{b4P0ZY5mKGyndnXsdy=> MUWyNlgzPTN3NR?=
NTera-2 M3;TNmZ2dmO2aX;uJGF{e2G7 NVrZW3JjOTJyIN88[{9udA>? NWCwVW57PzJiaB?= NWe2dVl2e2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgWGJCWlNibHX2[Yx{ MnLCNlI5OjV|NUW=
NCCIT M{HZZ2Z2dmO2aX;uJGF{e2G7 NIXIe4gyOjBizsznM41t M{jH[|czKGh? MXHzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCWQlHSV{Bt\X[nbIO= NVX1WGVSOjJ6MkWzOVU>
NCCIT MnXBSpVv[3Srb36gRZN{[Xl? MVexNlAh|rypL33s MnTkO|IhcA>? MUjzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCJU1igcIV3\Wy| MViyNlgzPTN3NR?=
NTera-2 Mn\0SpVv[3Srb36gRZN{[Xl? Mn\uNVIxKM7:Zz;tcC=> NV7ZOIpnPzJiaB?= MYPzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCScn;0[YlvKGOjcnLvcpltKGyndnXsdy=> MUCyNlgzPTN3NR?=
NCCIT M{TROmZ2dmO2aX;uJGF{e2G7 MXGxNlAh|rypL33s MoH4O|IhcA>? NVflNHdPe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgVJJwfGWrbjDjZZJjd267bDDs[ZZmdHN? NIeyVmIzOjh{NUO1OS=>
NTera-2 NU[5[YJyTnWwY4Tpc44hSXO|YYm= M2LXVFEzOCEQvHevcYw> NGDjSG44OiCq NUfUVIhve2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgUHBQKGyndnXsdy=> MoLnNlI5OjV|NUW=
NCCIT NHXFTZRHfW6ldHnvckBCe3OjeR?= NV7jXZVmOTJyIN88[{9udA>? NGnVUII4OiCq Mmnrd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiTGDPJIxmfmWucx?= MUCyNlgzPTN3NR?=
MDA-MB-468 NID5V4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjMSWZCOOLCk{KwNOKh|rypL33M MViyOEBp MYDpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 NFW0TIEzOjhzOUG5Oi=>
231-H2N NXfycoxyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nIXFDjiJN{MEFCpO69\y:vTB?= MX6yOEBp M3jmTYlvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> MV[yNlgyQTF7Nh?=
A549  M3PreGZ2dmO2aX;uJGF{e2G7 Mlz5NlAxNzRyMDFOwG0> MWjy[ZN2dHS|IHnuJIEh\G:|ZT3y[ZNxd26|aY\lJIlv[3KnYYPlJIlvKFKRUx?= MWeyNlc4OzZ7Nx?=
A549  MnXISpVv[3Srb36gRZN{[Xl? NIP4OWQxNTRyMDFOwG0> NIHuVnozNTR6IHi= MYHjZZV{\XNibX;y[UBufESQQTD0bIFvKG6GTlGg[IFu[Wen Mnu3NlI4PzN4OUe=
A549  M1LHc2Z2dmO2aX;uJGF{e2G7 NI\wUHQyODBizszNxsA> NVjkeYhDOOLCk{S4JIg> M1K4U4NifXOnczDjZZNx[XOnLUOgZYN1cX[jdHnvci=> MoTvNlI4PzN4OUe=
A549  MkWzSpVv[3Srb36gRZN{[Xl? M1TNbVExOCEQvF5CpC=> NUXGOnhSOOLCk{S4JIg> MXrjZZV{\XNiUFvENUBkdGWjdnHn[S=> NV\wVIc5OjJ5N{O2PVc>
A549  M3f5PGZ2dmO2aX;uJGF{e2G7 MluxNVAxKM7:TdMg NU[5UVhOOOLCk{S4JIg> M3zScYNifXOnczDtbZRw[2ixbnTybYFtKGyxY3HsbZpifGmxbjDv[kBD[Xh? MWOyNlc4OzZ7Nx?=
A549  NVzNS5hiTnWwY4Tpc44hSXO|YYm= MUOxNFAh|ryPwrC= M1LRdFDjiJN2ODDo MXjk[YNz\WG|ZYOgUW1RKGGwZDDBWHAhdGW4ZXzz M3PSdFIzPzd|Nkm3
MCF-7/Her-18  M3jBNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LocFAvPDJvMUewNEDPxGdxbVy= MkfWNlQhcA>? M{S5eYlvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> MonpNlI3OjF2MES=
MCF-7 Mo\hVGRVN1CFSTDUdoVifG2nboS= NYXxU5JnOC5{NTFOwIcwdWxw M1PiXVEuPCCq MYLzbI94eyC|aXfubYZq[2GwdDCoVOKhRCByLkC1LUBkgXSxdH;4bYMh\W[oZXP0 Mmf2NlI2QTF{OES=
MDA-MB-231 M2eybHBFXC:SQ1mgWJJm[XSvZX70 NIH5b5IxNjJ3IN88[{9udC5? NUjGUW85OS12IHi= MXHzbI94eyC|aXfubYZq[2GwdDCoVOKhRCByLkC1LUBkgXSxdH;4bYMh\W[oZXP0 NHLKSVAzOjV7MUK4OC=>
MDA-MB-235  MXXQSHQwWEOLIGTy[YF1dWWwdB?= M3W0fVAvOjVizsznM41tNg>? NIDHVHEyNTRiaB?= MU\zbI94eyC|aXfubYZq[2GwdDCoVOKhRCByLkC1LUBkgXSxdH;4bYMh\W[oZXP0 Mm\nNlI2QTF{OES=
MCF-7 M1jXN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LDc|AuOiEQvHevcYwv M{PDTVI1NzR6IHi= NYHzS|dETUN3ME2xMlIh|rypL33M NWjsN2txOjJ3OUGyPFQ>
NCCIT  NVX0eXhOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rqSFIx6oDVMUSwxsDPxGdxbXy= M1PScFczKGh? M4fF[WxFPTB;MUKwxsDPxGdxbX|CpC=> NFvaS5IzOjV4MkG2NC=>
NCCIT  MYfGeY5kfGmxbjDBd5NigQ>? MVK3NkBp M2\OeJNq\26rZnnjZY51dHliaX7jdoVie2W|IHPhd5Bie2VvMzDhZ5Rqfmm2edMg NInmbZQzOjV4MkG2NC=>
NCCIT  NFGzbGdHfW6ldHnvckBCe3OjeR?= Ml7kO|IhcA>? NV7KNGhZe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgZ4F{eGG|ZT24JIFkfGm4aYT5xsA> NGC5VnEzOjV4MkG2NC=>
NCCIT  NE\oZ5lHfW6ldHnvckBCe3OjeR?= MUe3NkBp NYXqN3Foe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgZ4F{eGG|ZT25JIFkfGm4aYT5xsA> NGq4bJczOjV4MkG2NC=>
NCCIT  MWnGeY5kfGmxbjDBd5NigQ>? NX3MR49QPzJiaB?= NHTTemp{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDCRXghdGW4ZXy= NITaWoEzOjV4MkG2NC=>
NCCIT  MXTGeY5kfGmxbjDBd5NigQ>? NH\udlc4OiCq NX;kSZFGe2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgeIhmKEKlbD2yJINwdnSnboS= MWWyNlU3OjF4MB?=
NCCIT  NHnGTmpHfW6ldHnvckBCe3OjeR?= M4XYSFczKGh? NIjvS5p{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUhS3m2LXOgcIV3\Wx? MX[yNlU3OjF4MB?=
HeLa MXjGeY5kfGmxbjDBd5NigQ>? NHn3TYI{OC95MDFOwIcwdWx? MXKyOOKhcA>? Mo[1bY5kemWjc3XzJJRp\SCvUl7BJIxmfmWuczDv[uKheDV|78{MRXRONHB{MdMgZY5lyqCJQVTEOFU> MkjGNlI1QDd7M{e=
MCF-7  M3P6SGZ2dmO2aX;uJGF{e2G7 M1XkRVMxNzdyIN88[{9udA>? MoPHNlTDqGh? NGPSOHVqdmO{ZXHz[ZMhfGinIH3SUmEhdGW4ZXzzJI9nyqCyNURvwKxCXE1ucEKxxsBidmUEoFfBSGQ1PQ>? MmrNNlI1QDd7M{e=
HeLa MnzrR5l1d3SxeHnjbZR6KEG|c3H5 MVOzNE84OCEQvHevcYw> NFWwdogzPMLiaB?= MUPpcoR2[2W|IHjp[4htgSC|aXfubYZq[2GwdDDs[ZZmdHNib3[g[pJi\22nboTl[EBFVkFicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21? NUD0dnZ3OjJ2OEe5N|c>
MCF-7  MoL4R5l1d3SxeHnjbZR6KEG|c3H5 MVKzNE84OCEQvHevcYw> MnTnNlTDqGh? MVLpcoR2[2W|IHjp[4htgSC|aXfubYZq[2GwdDDs[ZZmdHNib3[g[pJi\22nboTl[EBFVkFicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21? NF;RWGIzOjR6N{mzOy=>
NT2 NFfPbVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\hXYc5OTByLU[wNOKh|rypL33s Ml;BNlQhcA>? MmnXTWM2OD12MECgxtVoN22u MX[yNlQ3QTl3Mh?=
NT2 NYDWfFhDTnWwY4Tpc44hSXO|YYm= M{e0[|QxOMLizsznM41t MXSyOEBp NHK1UYF{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDjZZNx[XOnLUOsPEw6KGGldHn2bZR6yqB? NInE[nAzOjR4OUm1Ni=>
NT2 MnnpSpVv[3Srb36gRZN{[Xl? NEC0[pk1ODEEoN88[{9udA>? NEnsR4QzPCCq MXfzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCEQWigcIV3\Wx? NEnkN48zOjR4OUm1Ni=>
NT2 NIf6UJZHfW6ldHnvckBCe3OjeR?= M1XGTFQxOMLizsznM41t NITvVYMzPCCq MWHzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCEY3ytNkBt\X[nbB?= NXjPbXEyOjJ2Nkm5OVI>
CHO M1mxS2Z2dmO2aX;uJGF{e2G7 MmPHNk42yqEQvHevcYw> M2rjTVE5KGhxNjDk NXz3XGIycW6mdXPld:KheGW{c3nzeIVv[2Vib3[gZ4hzd22xc3;t[UBl[W2jZ3W= M1HuVlIzOjNyMUm1
Jurkat MmDlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\DZWgxNTFyMNMg{txoN22u NXX2NnhUOjRiaB?= NYfDTpQ1[XK{ZYP0d{B1cGViY3XscEBkgWOuZTDheEB1cGViR{KgdIhie2V? NXjSeIFyOjJ{MkOzN|I>
Jurkat Mn60SpVv[3Srb36gRZN{[Xl? NW[z[nprOzEEoN88[{9udA>? NHXjUowzPCCq M{m2NYlv[3KnYYPld{B1cGVicILveIVqdiCuZY\lcJMhd2ZiY4njcIlveyCGMzygRUBidmRiQkG= NU\OdmMzOjJ{MkOzN|I>
Jurkat NUPjXnRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXH4bnh5OC1zMEFCpO69\y:vbB?= M{nzPVI1KGh? NFfrVmRqdmirYnn0d{Bk\WyuIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhf2m2aDDhckBKSzVyII\hcJVmKG:oIEGwNO69\y:vTB?= NVnFeJN{OjJ{MkOzN|I>
Jurkat NIPyS4tHfW6ldHnvckBCe3OjeR?= MojkN|DDqM7:Zz;tcC=> MkTSNlQhcA>? M17ke4lv[3KnYYPld{B1cGViZX\m[YN1KG:oIID5Z45q\GmxbnWgc44heGixc4Doc5J6dGG2aX;uJIxmfmWuczDv[kBpcXO2b37lJGgzSS6[ M1n5PFIzOjJ|M{Oy
U2OS EGFPnls MkP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\1NE0zyqEQvHevcYw> Mm\vNlQhcA>? Ml33bY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> NFjmb2wzOThzMUCwOy=>
U2OS KuEnls MnTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\SVJAxNTMEoN88[{9udA>? MXmyOEBp NF31N2hqdmirYnn0d{Bk\WyuIHfyc5d1cCCmb4PlJIRmeGWwZHXueIx6 M2DWWlIyQDFzMEC3
MCF-7 NHLjOHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTlNE01ODBizszN MoXRO|IhcA>? MWjMR|UxRTF3MT64xsDPxE1? NY\5WJVYOjF5MEOyPVE>
MCF-7/Adr  NFj5TlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1OySlAuPDByIN88US=> M3PLdFczKGh? M4jXOmxEPTB;NUiuOeKh|ryPwrC= MVSyNVcxOzJ7MR?=
WI-38 NF25cFlHfW6ldHnvckBCe3OjeR?= MnnRNE81OC96MPMAje69\y:vbNMg NV3iSIROOyCq MVvpcoR2[2W|IHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRiaX7jdoVie2ViaX6geIhmKE2QIH\y[ZF2\W6ledMg NXvqOXlEOjF3OUm2NVI>
hBMSC MWDGeY5kfGmxbjDBd5NigQ>? NEX3NmExNzRyL{iw5qCK|rypL33sxsA> NEHNN2M{KGh? M3L3T4lv\HWlZYOgZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcoNz\WG|ZTDpckB1cGViTV6g[pJmeXWnbnP5xsA> NY\2[IN[OjF3OUm2NVI>
NCI-H23 MV\GeY5kfGmxbjDBd5NigQ>? Mni4NE81OC96MPMAje69\y:vbNMg NUHXdpc2OyCq MkXMbY5lfWOnczDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IHnuZ5Jm[XOnIHnuJJRp\SCPTjDmdoVyfWWwY4pCpC=> M4LwfFIyPTl7NkGy
A-549 MkHpSpVv[3Srb36gRZN{[Xl? MUGwM|QxNzhy4pEJ{txoN22uwrC= Mo\rN{Bp NED4ZlFqdmS3Y3XzJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgbY5kemWjc3WgbY4hfGinIF3OJIZz\XG3ZX7jfeKh M1rkS|IyPTl7NkGy
PBL  NX;1R|VwTnWwY4Tpc44hSXO|YYm= MWGwM|QxNzhy4pEJ{txoN22uwrC= NV\1dFV7OyCq M{jiPYlv\HWlZYOgZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcoNz\WG|ZTDpckB1cGViTV6g[pJmeXWnbnP5xsA> M1PxOlIyPTl7NkGy
pol β −/− NWfxO2oyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoT5NE4zPS1{MEFCpO69\y:vbB?= MlrrNlTDqGh? NV;tR2hV\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JJdqfGhiaX7jdoVie2mwZzDjc45k\W62cnH0bY9ve8Li NWHRd4pVOjF{NUG5OFQ>
pol β +/+ NV6xV4xtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHsToVWOC5{NT2yNFDDqM7:Zz;tcC=> NFTVNIkzPMLiaB?= MXvk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHlid3n0bEBqdmO{ZXHzbY5oKGOxbnPlcpRz[XSrb37zxsA> M4KyXlIyOjVzOUS0
pol β −/− M4ixZmZ2dmO2aX;uJGF{e2G7 NIjXRnoxNTJwNTFOwIcwdWx? MnvaNkBp MUTjZZV{\XNiRF7BJIRidWGpZR?= NHzvRo0zOTJ3MUm0OC=>
pol β +/+ NHrTRlNHfW6ldHnvckBCe3OjeR?= NGjndpMxNTJwNTFOwIcwdWx? MonnNkBp MXLjZZV{\XNiRF7BJIRidWGpZR?= MXSyNVI2OTl2NB?=
pol β −/− NH\OW2dHfW6ldHnvckBCe3OjeR?= NFzkTXUxNTVizsznM41t MlfoOFghcA>? MmG2Z4F2e2W|IH3pZ5JwdnWlbHX1d{Bld3OnIHHu[EB1cW2nIHTldIVv\GWwdHz5 NHm5[IQzOTJ3MUm0OC=>
pol β +/+ M{PPW2Z2dmO2aX;uJGF{e2G7 MUGwMVUh|rypL33s MnjxOFghcA>? MWPjZZV{\XNibXnjdo9vfWOuZYXzJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= MmLlNlEzPTF7NES=
TK6  NU\UdIJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmH3NlUuOTVyIN88[{9udA>? NWjJOm9pQTZiaB?= Mm\pbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MWqyNVA5OTR6Nx?=
TK6  sLUC+Apn1 M1znWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUmyOU0yPTBizsznM41t NWHD[3djQTZiaB?= M{XobolvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> NVjEWldHOjFyOEG0PFc>
TK6 sAPE1+Apn1 NUO4SWdvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nLclI2NTF3MDFOwIcwdWx? MkTXPVYhcA>? M4PlTJNq\26rZnnjZY51dHliaX7obYJqfHNiY3XscEBoem:5dHig[I9{\SCmZYDlcoRmdnSueR?= MmLSNlExQDF2OEe=
HCT116 NVPNXJo3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\mclI2NTF3MDFOwIcwdWx? M330U|k3KGh? MVjpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 NXrVeYR7OjFyOEG0PFc>
HCT116 sLUC+Apn1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rKV|I2NTF3MDFOwIcwdWx? NX71RoRDQTZiaB?= MkXCbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MlH4NlExQDF2OEe=
HCT116 sAPE1+Apn1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIOx[VAzPS1zNUCg{txoN22u NXLlT4x7QTZiaB?= MorKd4lodmmoaXPhcpRtgSCrbnjpZol1eyClZXzsJIdzd3e2aDDkc5NmKGSncHXu[IVvfGy7 MV2yNVA5OTR6Nx?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LRP6 / Wnt5AB / Dvl2 / β-catenin; 

PubMed: 27649046     


CRL-1490 cells were treated with the indicated concentrations of bleomycin for either 12 or 24 h, and then probed for canonical Wnt proteins LRP6, Wnt5AB, Dvl2, and β-catenin. 

phospho-eIF2α / eIF2α; 

PubMed: 23762310     


Western blotting analysis of phosphorylated, total eIF2α and β-actin in CT26 treated 24 h with the indicated drugs. BLM: Bleomycin

cytochrome c; 

PubMed: 20154224     


mitochondrial release of cytochrome c was detected by Western blot in cytosolic extract and analyzed by band densitometry. Data show the means of four independent experiments ± SE. *Statistical significance from control, P < 0.05. Representative Western blots are shown.

Bcl-xl; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-xL.

Bcl-2; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-2.

27649046 23762310 20154224
Immunofluorescence
LC3; 

PubMed: 23762310     


CT26 cells were cultured on glass slides and treated with indicated drugs for 24 h. The cells were then submitted to anti-LC3 (green) and DAPI (blue) labeling and observed under an epifluorescence microscope. BLM: Bleomycin

23762310
ELISA
HMGB1; 

PubMed: 23762310     


HMGB1 was titrated with ELISA method in supernatant of CT26 cells treated 24 h with indicated drugs. BLM: Bleomycin

23762310
In vivo Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21. [5]

Protocol

Cell Research:

[4]

- Collapse
  • Cell lines: ADIPO-P2 cells
  • Concentrations: 2.5 μg/mL
  • Incubation Time: 30 minutes
  • Method:

    ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH. Two independent experiments are carried out.


    (Only for Reference)
Animal Research:

[7]

- Collapse
  • Animal Models: CD-1 mice
  • Dosages: 5 mg/kg, 2 ml/kg
  • Administration: Administered via i.t.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (66.11 mM)
Water 100 mg/mL (66.11 mM)
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
Saline
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1512.62
Formula

C55H85N17O25S4

CAS No. 9041-93-4
Storage powder
in solvent
Synonyms NSC125066
Smiles CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)OC3C(C(C(C(O3)CO)O)O)OC4C(C(C(C(O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    what is the ratio between bleomycin A2 and bleomycin B2 in your product?

  • Answer:

    The content of Bleomycin A2 and Bleomycin B2 is 62% and 29% respectively.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID