Bleomycin (NSC125066) sulfate

Catalog No.S1214

For research use only.

Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.

Bleomycin (NSC125066) sulfate Chemical Structure

CAS No. 9041-93-4

Selleck's Bleomycin (NSC125066) sulfate has been cited by 70 publications

Purity & Quality Control

Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.
In vitro

UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. [1] Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL Bleomycin sulfate for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without Bleomycin sulfate. Macrophages stimulated with Bleomycin sulfate continues to produce significant amounts of fibroblast growth factor even after Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by Bleomycin sulfate-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. [2] Bleomycin sulfate (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. [3] In terms of unstable aberrations, the clastogenic effect of Bleomycin sulfate on ADIPO-P2 cells persists for at least 10 days after exposure. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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POLK WT NWrV[3NiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLYdJFKSzVyPUGxMlfjiIoEsfMAjVEvP8LizszN MlrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyM{G0NFAoRjJ4MEOxOFAxRC:jPh?=
POLK KO NIj0NXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz2TWM2OD12LkCz5qCKyrIkgJmxMlkzyqEQvF2= MnXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyM{G0NFAoRjJ4MEOxOFAxRC:jPh?=
POLK CD M4XrWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknMTWM2OD13LkW55qCKyrIkgJmxMlAxyqEQvF2= NXfzWoRvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wN|E1ODBpPkK2NFMyPDByPD;hQi=>
RLE/Abca3 NIL3OVdHfW6ldHnvckBCe3OjeR?= NVHuNFRuOC52wrFOwG0> MkDiNVQ1yqCq NHnhR3NqdmS3Y3XzJIEhdW:{cHjvcI9ocWOjbDDjbIFv\2ViZoLvcUBmeGm2aHXsbYFtNWyra3WgcY9zeGixbH;nfUB1dyCjIIfp[IUtKHOycnXh[E1wfXRibX;ydIhwdG:pedMg MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4MEWzPEc,OjV5NkC1N|g9N2F-
PMCs MnXYSpVv[3Srb36gRZN{[Xl? MU[wMlHjiJN{wrFOwIcwdWx? MUOyOOKhcA>? MXPjZZV{\XNiYTDkc5NmNWSncHXu[IVvfCCrbnPy[YF{\SCrbjD0bIUheHKxdHXpckBt\X[nbDDv[kBkd2yuYXflck1KyqB? M2rIRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm1OlQzLz5{NUW5OVY1OjxxYU6=
PMCs NXHqdmdVTnWwY4Tpc44hSXO|YYm= NGPFWJcxNjMEoN88[{9udA>? NGPibpAzPOLCk{eyxsBp Ml3RbY5lfWOnczDpcoNz\WG|ZYOgbY4hfmmvZX70bY4h[W6mIN8xMXNOSSCycn;0[Ylv NHf6R2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5OVY1Oid-MkW1PVU3PDJ:L3G+
PMCs NYTpR2Z[TnWwY4Tpc44hSXO|YYm= NFPDWXMxNjMEoN88[{9udA>? MnrMO|IhcA>? NHi3PG9qdmS3Y3XzJIRm[3KnYYPld{BqdiCleYTvb4Vz[XSrbj24JIFv\CCHLXPh[IhmemmwIIDyc5RmcW5? MmCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUW2OFIoRjJ3NUm1OlQzRC:jPh?=
PMCs M3;3ZmZ2dmO2aX;uJGF{e2G7 MkOzNE4zyqEQvHevcYw> NWrJ[npbOTJxMkSvOFghcA>? MXLpcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? M1q0clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm1OlQzLz5{NUW5OVY1OjxxYU6=
IMR-32 NGD3bmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjo[m51Py534pkSNVIxKML3Zz;tcC=> MUmxNwKJmjR6IHi= NHiyS3hKSzVyPU[wJOK2\y:vbDDheEB1cGViZX7kJI9nKDJ2IHjyd{Bw\iCrbnP1ZoF1cW:w NHvWfYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzOlM1PSd-MkW1N|Y{PDV:L3G+
HT1080 NXjGbmo1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HubVEh|ryP NHnsZVAzPCCqwrC= MWns[YFleyC2bzDhJGczN01iZHXsZZk> M3PofVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUG4PVYyLz5{NUWxPFk3OTxxYU6=
HT1080 NGrFWJlHfW6ldHnvckBCe3OjeR?= NIPhc2EyKM7:TR?= Mn;CNlQhcMLi NFvlV2FqdmS3Y3XzJIEheG:2ZX70JIdtd2KjbDDEUmEh\GGvYXflJJJme3CxboPl MnnZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3MUi5OlEoRjJ3NUG4PVYyRC:jPh?=
HDFn MYHDfZRwfG:6aXPpeJkhSXO|YYm= MmjTO|IhcA>? MnH0TWM2OD17LkOxKeKh NGOxXVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK3Olc6Oid-MkWyO|Y4QTJ:L3G+
THP-1 M3HGemN6fG:2b4jpZ4l1gSCDc4PhfS=> MlfNO|IhcA>? NX6zXJhCUUN3ME20Mlc4LQ>? M1PvdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke2O|kzLz5{NUK3Olc6OjxxYU6=
HT-29 M1fVeWN6fG:2b4jpZ4l1gSCDc4PhfS=> MWq3NkBp NGH4dZhKSzVyPUGxMlQ6LQ>? M1jIO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mke2O|kzLz5{NUK3Olc6OjxxYU6=
HCT116  NUHRVIxTS3m2b4TvfIlkcXS7IFHzd4F6 Ml7rO|IhcA>? NV7sUmNFUUN3ME2xNU4{PCV? NVnRW|hbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyO|Y4QTJpPkK1Nlc3Pzl{PD;hQi=>
A431 MnnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;yZ2FrPTBvMkCwJGlW NUi2TIRLPDkkgJno M{naeYlvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> M4[wc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUCxNlk6Lz5{NUGwNVI6QTxxYU6=
T24 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{D2TlUxNTJyMDDJWS=> MXK0PQKBkWh? MYjpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 MkPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEGyPVkoRjJ3MUCxNlk6RC:jPh?=
AY-27  M2H2T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzaOVAuOjByIFnV NWTXXFBDPDkkgJno MYrpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 MoS3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMEGyPVkoRjJ3MUCxNlk6RC:jPh?=
A549 MkW4SpVv[3Srb36gRZN{[Xl? MU[xJOK2\y:vTNMg MmHmNE01QCCq NHi1OGJqdmO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4bDqE:STtMgcXJPSQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4M{[zOUc,OjR7NkO2N|U9N2F-
MLE12 MVXGeY5kfGmxbjDBd5NigQ>? NUnufFh{OSEEtXevcWzDqA>? MXewMVQ5KGh? MUXpcoNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3dCpG9RVsLibWLORS=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4M{[zOUc,OjR7NkO2N|U9N2F-
Jurkat MorWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4j3O|Ix6oDLzszt M{jzNFI16oDLaNMg NH7ZO2tienKnc4TzJJRp\SClZXzsJIN6[2ynIHH0JJRp\SCJMjDwbIF{\Q>? NYPnTY1vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVY5QTNpPkK0PVE3QDl|PD;hQi=>
HeLa  MmrKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPL[ZVWUUN3ME2xNE4zyqEQvF2= NFnBUVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEezNlM6Pyd-MkS3N|I{QTd:L3G+
C18-4 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjlc|QxNTFyMDFOwG0> M2S1eolvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> MlXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3N{G5PFIoRjJ2NUexPVgzRC:jPh?=
BMG-1  NF\iXnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGq1NJY1OC96MNMg{txoN21? NEj1c4Q{yqCq NFrVXHlqdmirYnn0d{Bk\WyuIHfyc5d1cCCmb4PlJIRmeGWwZHXueIx6 NEXTcYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOlczPid-MkO5NFY4OjZ:L3G+
A459 NIG3[2lCeG:ydH;zbZMhSXO|YYm= MmqwNVAhdVV? MVG0PEBp M4LUb4lv\HWlZYOgZZBweHSxc3nz NYrrT3c5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFI4PjZpPkKzPVAzPzZ4PD;hQi=>
MOCK NGnsNXpCeG:ydH;zbZMhSXO|YYm= NVrH[oo5OTBibWW= NHnoTI41QCCq MkjhbY5lfWOnczDhdI9xfG:|aYO= NXLGNFhLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFI4PjZpPkKzPVAzPzZ4PD;hQi=>
MMP1 NI[4d|RCeG:ydH;zbZMhSXO|YYm= M2DpO|ExKG2X NWGzcIdUPDhiaB?= MVfpcoR2[2W|IHHwc5B1d3Orcx?= NYPoNlBwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFI4PjZpPkKzPVAzPzZ4PD;hQi=>
A549 NVL2SJJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLFXVd2OjByIN88US=> NFmyT40zPCCqwrC= Mm\s[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> M4jyS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC1O|k{Lz5{M{iwOVc6OzxxYU6=
HCT-116 MVzBdI9xfG:|aYOgRZN{[Xl? M1y0UlMwPy53L{G1JO69\y:vbB?= MXm0PEBp M3KxcZJm\HWlZYOgeIhmKGyndnXsJI9nKEKOSDDheEBpcWeqIHPvcoNmdnS{YYTpc44> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyOE[2PEc,OjN5MEi2Olg9N2F-
HeLa MXrBdI9xfG:|aYOgRZN{[Xl? M16zbVMwPy53L{G1JO69\y:vbB?= NVL4dVFIPDhiaB?= NGXn[45z\WS3Y3XzJJRp\SCuZY\lcEBw\iCETFigZZQhcGmpaDDjc45k\W62cnH0bY9v MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyOE[2PEc,OjN5MEi2Olg9N2F-
HCT116 NIWzbINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoflNE0yODBizsznM41t NWXZU441PDhiaB?= MVPpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 NVjEenVURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVgzODFpPkKzOVE5OjBzPD;hQi=>
NCM460 NUfkWGZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDSUWIxNTFyMDFOwIcwdWx? NVG5SJByPDhiaB?= M12wRolvcGmkaYTzJINmdGxiZ4Lve5RpKGSxc3Wg[IVx\W6mZX70cJk> NYHieFZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NVgzODFpPkKzOVE5OjBzPD;hQi=>
NT2  NYnnWIlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYeyOEBpyqB? MVnMSFUxRTRyMPMAjeK2\y:vbB?= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN6NkSyNEc,OjN|OE[0NlA9N2F-
NT2  NVPRU|ZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXlOFghcA>? M4jmVGxFPTB;MUCwJOK2\y:vbB?= NVrxNXo5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzPFY1OjBpPkKzN|g3PDJyPD;hQi=>
NT2  MlnvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUi3NkBp MYDMSFUxRTJy4pEJxtVoN22u M4\HeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{i2OFIxLz5{M{O4OlQzODxxYU6=
NT2  NUn0PZF5SXCxcITvd4l{KEG|c3H5 MW[0NFDjiIoEtXevcYw> MnfxNlQhcMLi M2DjVIlv\HWlZYOgd4lodmmoaXPhcpQhcW6lcnXhd4V{KGmwIHPhd5Bie2VvMzy4MFkh[WO2aY\peJnDqA>? MnvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|OE[0NlAoRjJ|M{i2OFIxRC:jPh?=
NTera-2 NV3MNoQyTnWwY4Tpc44hSXO|YYm= MV:xNlAh|rypL33s MoDWO|IhcA>? NWfqeIlke2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgPE1qe2:ycn;zeIFv\SCuZY\lcJM> MoDBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MkWzOVUoRjJ{OEK1N|U2RC:jPh?=
NCCIT NHrvPIxHfW6ldHnvckBCe3OjeR?= NVjwNVBQOTJyIN88[{9udA>? NULvNZV5PzJiaB?= NIDmU2t{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczC4MYl{d3C{b4P0ZY5mKGyndnXsdy=> M3fPe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK1N|U2Lz5{MkiyOVM2PTxxYU6=
NTera-2 MV7GeY5kfGmxbjDBd5NigQ>? MnvvNVIxKM7:Zz;tcC=> M4PaN|czKGh? MkL0d4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiVFLBVnMhdGW4ZXzz NYH3NVhERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NCCIT MYDGeY5kfGmxbjDBd5NigQ>? NIDncIcyOjBizsznM41t MmDTO|IhcA>? M3jMSJNq\26rZnnjZY51dHliaX7jdoVie2W|IGTCRXJUKGyndnXsdy=> NUT6UoxPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NCCIT MkP0SpVv[3Srb36gRZN{[Xl? MYWxNlAh|rypL33s M2XBSVczKGh? MWPzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyCJU1igcIV3\Wy| NWXrelZURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NTera-2 M2nJVmZ2dmO2aX;uJGF{e2G7 Mo\yNVIxKM7:Zz;tcC=> MXy3NkBp MV7zbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCScn;0[YlvKGOjcnLvcpltKGyndnXsdy=> NVTzRZVsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NCCIT Mn[zSpVv[3Srb36gRZN{[Xl? MoXLNVIxKM7:Zz;tcC=> MVK3NkBp NIe2Zm1{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDQdo91\WmwIHPhdoJwdnmuIHzleoVtew>? NHO0Z|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVM2PSd-MkK4NlU{PTV:L3G+
NTera-2 NV;ieVlsTnWwY4Tpc44hSXO|YYm= M3XiTVEzOCEQvHevcYw> NH\1SYQ4OiCq Mn2yd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiTGDPJIxmfmWucx?= NGDtXW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVM2PSd-MkK4NlU{PTV:L3G+
NCCIT NVXyRXhlTnWwY4Tpc44hSXO|YYm= NIHUVokyOjBizsznM41t NUj5SXM4PzJiaB?= Mofhd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiTGDPJIxmfmWucx?= M4rnd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK1N|U2Lz5{MkiyOVM2PTxxYU6=
MDA-MB-468 MmrTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELzS|Mx6oDVMkCwxsDPxGdxbVy= MonUNlQhcA>? MWDpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhzOUG5Okc,OjJ6MUmxPVY9N2F-
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A549  NH2xT2NHfW6ldHnvckBCe3OjeR?= NYrEWoJKOjByL{SwNEDPxE1? NH3pPVdz\XO3bITzJIlvKGFiZH;z[U1z\XOyb37zbZZmKGmwY4LlZZNmKGmwIGLPVy=> NX76RW9yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3O|M3QTdpPkKyO|c{Pjl5PD;hQi=>
A549  MoKxSpVv[3Srb36gRZN{[Xl? MlL1NE01ODBizszN MX2yMVQ5KGh? NU\kS5Iz[2G3c3XzJI1wemVibYTEUmEhfGijbjDuSG5CKGSjbXHn[S=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd5M{[5O{c,OjJ5N{O2PVc9N2F-
A549  MV;GeY5kfGmxbjDBd5NigQ>? MofINVAxKM7:TdMg MlrWNQKBmzR6IHi= M2fyWINifXOnczDjZZNx[XOnLUOgZYN1cX[jdHnvci=> MlzCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{O2PVcoRjJ{N{ezOlk4RC:jPh?=
A549  MYrGeY5kfGmxbjDBd5NigQ>? NIn1XFcyODBizszNxsA> NI\adlEx6oDVNEigbC=> NEDicpNk[XW|ZYOgVGtFOSClbHXheoFo\Q>? M4rSd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{ezOlk4Lz5{Mke3N|Y6PzxxYU6=
A549  MUHGeY5kfGmxbjDBd5NigQ>? NIrTZngyODBizszNxsA> NI\vWWEx6oDVNEigbC=> NHTnUnFk[XW|ZYOgcYl1d2Oqb37kdolidCCub3PhcIl7[XSrb36gc4YhSmG6 MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd5M{[5O{c,OjJ5N{O2PVc9N2F-
A549  MlLNSpVv[3Srb36gRZN{[Xl? Ml\iNVAxKM7:TdMg M1jZXVDjiJN2ODDo NW\jfIw4\GWlcnXhd4V{KE2PUDDhcoQhSVSSIHzleoVtew>? MorMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{O2PVcoRjJ{N{ezOlk4RC:jPh?=
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MDA-MB-235  MVPQSHQwWEOLIGTy[YF1dWWwdB?= NInScWoxNjJ3IN88[{9udC5? MXSxMVQhcA>? NGP2[ZR{cG:5czDzbYdvcW[rY3HueEApWMLiPDCwMlA2MSCleYTveI95cWNiZX\m[YN1 M3H3UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUmxNlg1Lz5{MkW5NVI5PDxxYU6=
MCF-7 NYD2TI5bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUOwMVIh|rypL33sMi=> NFPG[IkzPC92ODDo NUHGV5B6TUN3ME2xMlIh|rypL33M M1PETFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUmxNlg1Lz5{MkW5NVI5PDxxYU6=
NCCIT  M{LWSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDXNoMzOOLCk{G0NOKh|rypL33s Ml\yO|IhcA>? NXPUVYlPVER3ME2xNlDDqM7:Zz;tcOKh M1HOUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[yNVYxLz5{MkW2NlE3ODxxYU6=
NCCIT  MV\GeY5kfGmxbjDBd5NigQ>? MnrGO|IhcA>? NWP0dFBLe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgZ4F{eGG|ZT2zJIFkfGm4aYT5xsA> MoGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkKxOlAoRjJ{NU[yNVYxRC:jPh?=
NCCIT  MYjGeY5kfGmxbjDBd5NigQ>? NWq1e2lZPzJiaB?= M1rGbZNq\26rZnnjZY51dHliaX7jdoVie2W|IHPhd5Bie2VvODDhZ5Rqfmm2edMg M4rxU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[yNVYxLz5{MkW2NlE3ODxxYU6=
NCCIT  NHXKc3BHfW6ldHnvckBCe3OjeR?= Mm\wO|IhcA>? M{XoR5Nq\26rZnnjZY51dHliaX7jdoVie2W|IHPhd5Bie2VvOTDhZ5Rqfmm2edMg M4P2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[yNVYxLz5{MkW2NlE3ODxxYU6=
NCCIT  NI\ZVVFHfW6ldHnvckBCe3OjeR?= MW[3NkBp MXLzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCEQWigcIV3\Wx? NGjmT2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW2NlE3OCd-MkK1OlIyPjB:L3G+
NCCIT  MYHGeY5kfGmxbjDBd5NigQ>? NYfsZYVqPzJiaB?= NXX5c5d4e2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgeIhmKEKlbD2yJINwdnSnboS= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV4MkG2NEc,OjJ3NkKxOlA9N2F-
NCCIT  NXjmPFVnTnWwY4Tpc44hSXO|YYm= NYL1TVdoPzJiaB?= Mlfhd4lodmmoaXPhcpRtgSCmZXPy[YF{\XNidHjlJGN6fC2lIHzleoVt MoHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkKxOlAoRjJ{NU[yNVYxRC:jPh?=
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MCF-7  M2P1ZmZ2dmO2aX;uJGF{e2G7 NUfINZl2OzBxN{Cg{txoN22u MWmyOOKhcA>? NEfLfnhqdmO{ZXHz[ZMhfGinIH3SUmEhdGW4ZXzzJI9nyqCyNURvwKxCXE1ucEKxxsBidmUEoFfBSGQ1PQ>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6N{mzO{c,OjJ2OEe5N|c9N2F-
HeLa MUDDfZRwfG:6aXPpeJkhSXO|YYm= MmPiN|AwPzBizsznM41t MVWyOOKhcA>? NXqxU2h[cW6mdXPld{BpcWeqbImgd4lodmmoaXPhcpQhdGW4ZXzzJI9nKG[{YXft[Y51\WRiRF7BJJJmdGWjc3WgbY51dyC2aHWgZ5l1d3CuYYPt NUHD[pZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFc6OzdpPkKyOFg4QTN5PD;hQi=>
MCF-7  MUPDfZRwfG:6aXPpeJkhSXO|YYm= Ml3ZN|AwPzBizsznM41t MYSyOOKhcA>? MXTpcoR2[2W|IHjp[4htgSC|aXfubYZq[2GwdDDs[ZZmdHNib3[g[pJi\22nboTl[EBFVkFicnXs[YF{\SCrboTvJJRp\SCleYTvdIxie21? M1nPZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEi3PVM4Lz5{MkS4O|k{PzxxYU6=
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NT2 NY\Fbmt4TnWwY4Tpc44hSXO|YYm= NYfhcoVKPDBywrFOwIcwdWx? MlnxNlQhcA>? NEXEcVB{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDjZZNx[XOnLUOsPEw6KGGldHn2bZR6yqB? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4OUm1Nkc,OjJ2Nkm5OVI9N2F-
NT2 NHXU[nhHfW6ldHnvckBCe3OjeR?= M4X1WlQxOMLizsznM41t NYP4dXh{OjRiaB?= NFXsc4J{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDCRXghdGW4ZXy= MnHNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Nkm5OVIoRjJ{NE[5PVUzRC:jPh?=
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Jurkat Mn;LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\IO|AuOTBywrFOwIcwdWx? MWKyOEBp M135SoFzemW|dIOgeIhmKGOnbHygZ5lkdGViYYSgeIhmKEd{IIDoZZNm NUH4O5hQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlM{OzJpPkKyNlI{OzN{PD;hQi=>
Jurkat M2Lwe2Z2dmO2aX;uJGF{e2G7 MWmzNOKh|rypL33s NEfGVYczPCCq M{nMXolv[3KnYYPld{B1cGVicILveIVqdiCuZY\lcJMhd2ZiY4njcIlveyCGMzygRUBidmRiQkG= M1m3WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkKzN|MzLz5{MkKyN|M{OjxxYU6=
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Jurkat MVTGeY5kfGmxbjDBd5NigQ>? MnjNN|DDqM7:Zz;tcC=> Mom1NlQhcA>? Mo\hbY5kemWjc3XzJJRp\SCnZn\lZ5Qhd2ZicInjcollcW:wZTDvckBxcG:|cHjvdplt[XSrb36gcIV3\Wy|IH;mJIhqe3SxbnWgTFJCNlh? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ{M{OzNkc,OjJ{MkOzN|I9N2F-
U2OS EGFPnls M4jT[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkP3NE0zyqEQvHevcYw> NUPkWGJDOjRiaB?= NV\SZ5NscW6qaXLpeJMh[2WubDDndo94fGhiZH;z[UBl\XCnbnTlcpRtgQ>? MojPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MUGwNFcoRjJzOEGxNFA4RC:jPh?=
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MCF-7/Adr  M4DvVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml2zNE01ODBizszN NGrxclQ4OiCq M3HCfGxEPTB;NUiuOeKh|ryPwrC= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyM{K5NUc,OjF5MEOyPVE9N2F-
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pol β +/+ MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXqwMlI2NTJyMNMg{txoN22u M1HDbFI1yqCq M{DPSoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTD3bZRpKGmwY4LlZZNqdmdiY3;uZ4VvfHKjdHnvcpPDqA>? NWrlUlFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVE6PDRpPkKxNlUyQTR2PD;hQi=>
pol β −/− NHfGTXhHfW6ldHnvckBCe3OjeR?= MoqwNE0zNjVizsznM41t NGrSbo0zKGh? MV;jZZV{\XNiRF7BJIRidWGpZR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ3MUm0OEc,OjF{NUG5OFQ9N2F-
pol β +/+ NWDne3ptTnWwY4Tpc44hSXO|YYm= NX\5TYZGOC1{LkWg{txoN22u MYCyJIg> MlrsZ4F2e2W|IFTORUBl[W2jZ3W= M{L1dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkWxPVQ1Lz5{MUK1NVk1PDxxYU6=
pol β −/− MXvGeY5kfGmxbjDBd5NigQ>? NXPjUFJuOC13IN88[{9udA>? MXO0PEBp NEm2VYJk[XW|ZYOgcYlkem:wdXPs[ZV{KGSxc3WgZY5lKHSrbXWg[IVx\W6mZX70cJk> M3zKSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkWxPVQ1Lz5{MUK1NVk1PDxxYU6=
pol β +/+ MVfGeY5kfGmxbjDBd5NigQ>? NX3rRndbOC13IN88[{9udA>? M3;IfVQ5KGh? M4jLS4NifXOnczDtbYNzd263Y3zleZMh\G:|ZTDhcoQhfGmvZTDk[ZBmdmSnboTsfS=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ3MUm0OEc,OjF{NUG5OFQ9N2F-
TK6  MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDQNlUuOTVyIN88[{9udA>? NU\QPJp[QTZiaB?= NU\oSGRVcW6qaXLpeJMh[2WubDDndo94fGhiZH;z[UBl\XCnbnTlcpRtgQ>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6MUS4O{c,OjFyOEG0PFc9N2F-
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2SC/20 M4\uVWZ2dmO2aX;uJGF{e2G7 MWS3NkBp M{fWRmlEPTBiPTCwMlUyPyEQvF2= M3LRWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NEOxNFYxLz5zMkSzNVA3ODxxYU6=
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COLO-668 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzq[GpKSzVyIE2gNE4xODByMkG4JO69VQ>? M1SwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
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RMG-I NHXLb4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;zTWM2OCB;IECuNFAxOTNzOTFOwG0> M{\BflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
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HCC1937 NXHCelQ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDyTWM2OCB;IECuNFAxOjV2IN88US=> NIqyfok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
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OVCAR-8 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlr5TWM2OCB;IECuNFAxPDd4MzFOwG0> M1y1[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
CESS Ml7uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxKD1iMD6wNFA2PDV5IN88US=> NYHVOXZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
NCI-H650 NEO2ZXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxKD1iMD6wNFA2QDd|IN88US=> NU\JeFFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
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RS4-11 MoTMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTXV2h2UUN3MDC9JFAvODBzNkSzJO69VQ>? NUm1VpNSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
MFH-ino NGmwPYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxKD1iMD6wNFI3PDZizszN NWPPWXZ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
KYSE-150 M3PSSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxKD1iMD6wNFI3PDdizszN MkHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
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Detroit562 MnLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHX3OY1KSzVyIE2gNVEvQTNzIN88US=> M2fJTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
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MDA-MB-453 NG[3U|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxKD1iMUWuOFE2PSEQvF2= NFX2UoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
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COLO-741 NHzROnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rmVGlEPTBiPTCxOk41OjJ5IN88US=> M4SwNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
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BB65-RCC NVvCcGFXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\zTWM2OCB;IEKxMlQzPjJizszN NXmzeZZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
EB-3 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfUNpRKSzVyIE2gNlEvQDN|MTFOwG0> MonEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
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NCI-H69 NHrpfmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i2b2lEPTBiPTCxNFMvQTB7IN88US=> NGqySJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
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NCI-H1581 MmG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELHW5lKSzVyIE2gNVExNjJ{NDFOwG0> NIX3VI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
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SKG-IIIa MluyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLBe5NKSzVyIE2gNVM3NjR4OTFOwG0> M2LFfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
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Mewo M3OzZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYSxSmRpUUN3MDC9JFE1Py57NkKg{txO MoT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
MSTO-211H MnW0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXoe3hKSzVyIE2gNVQ6NjR3OTFOwG0> NHG2eoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
Assay
Methods Test Index PMID
Western blot LRP6 / Wnt5AB / Dvl2 / β-catenin ; phospho-eIF2α / eIF2α ; cytochrome c ; Bcl-xl ; Bcl-2 27649046 23762310 20154224
Immunofluorescence LC3 23762310
ELISA HMGB1 23762310
In vivo Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21. [5]

Protocol (from reference)

Cell Research:

[4]

  • Cell lines: ADIPO-P2 cells
  • Concentrations: 2.5 μg/mL
  • Incubation Time: 30 minutes
  • Method:

    ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH. Two independent experiments are carried out.

Animal Research:

[7]

  • Animal Models: CD-1 mice
  • Dosages: 5 mg/kg, 2 ml/kg
  • Administration: Administered via i.t.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 1512.62
Formula

C55H85N17O25S4

CAS No. 9041-93-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)OC3C(C(C(C(O3)CO)O)O)OC4C(C(C(C(O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-]

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

Question 1:
what is the ratio between bleomycin A2 and bleomycin B2 in your product?

Answer:
The content of Bleomycin A2 and Bleomycin B2 is 62% and 29% respectively.

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