Barasertib (AZD1152-HQPA|AZD2811)

Catalog No.S1147 Synonyms: INH 34

Barasertib (AZD1152-HQPA|AZD2811) Chemical Structure

Molecular Weight(MW): 507.56

Barasertib (AZD1152-HQPA) is a highly selective Aurora B inhibitor with IC50 of 0.37 nM in a cell-free assay, ~3700 fold more selective for Aurora B over Aurora A. Phase 1.

Size Price Stock Quantity  
In DMSO USD 168 In stock
USD 120 In stock
USD 210 In stock
USD 670 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 26 Publications

10 Customer Reviews

  • Targeting PI3K, a common downstream effector of RTKs, with a selective inhibitor (GDC0941) sensitizes SOX10 knockdown cells to vemurafenib. shRNAs targeting SOX10 were introduced into A375 cells by lentiviral transduction. pLKO.1 empty vector served as a control vector (Ctrl). Cells were seeded in 6-well plates at the same density in the presence or absence of drug(s) at the indicated concentration. Cells were cultured for 2 weeks in the absence of vemurafenib or 4 weeks in the presence of vemurafenib before fixing and staining.

    Nature 2014 508(7494), 118-22. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

    Primary MKPs were treated with the Aurora B inhibitor AZD-1152, and then stimulated with 20 ng/ml TPO for 5 d. Cell morphology was analyzed by Giemsa staining (Bar, 20 祄; red arrows denote mature MKs; n = 6).

    J Exp Med 2014 10.1084/jem.20141123. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

  • The alamarBlue assay revealed that AURKB inhibition with AZD1152 was effective in NB TICs at EC50 of 1.5 to 4.6 μmol/L, whereas AURKB inhibition was effective in SKPs at 12.4 μmol/L.

    Clin Cancer Res 2010 16, 4572-4582. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

    : Barasertib inhibits AURKB specifically and triggers mitotic slippage. (a) Barasertib inhibits AURKB without affecting AURKA. Mitotic HeLa cells were obtained by exposure to nocodazole for 16 h followed by mechanical shake off. The cells were incubated with the indicated concentrations of Barasertib for 2 h. Nocodazole and MG132 were included to prevent mitotic exit. Lysates were prepared and analyzed with immunoblotting. Uniform loading was confirmed by immunoblotting for actin. (b) Barasertib induces mitotic slippage. HeLa cells expressing histone H2B-GFP were exposed to buffer or the indicated concentrations of Barasertib. Individual cells were then tracked for 24 h with time- lapse microscopy. Each horizontal bar represents one cell (n ¼ 50). The key is the same as in Figure 1b. (c) Summary of Barasertib-mediated mitotic slippage. Live-cell imaging after Barasertib treatment was described in panel (b). The duration of mitosis (mean±90% confidence interval) and the percentage of cells that underwent mitotic slippage during the imaging period were quantified. (d) Genome reduplication after Barasertib-mediated mitotic slippage. HeLa cells were treated with the indicated concentrations of Barasertib for 36 h. DNA contents were analyzed with flow cytometry. (e) Barasertib induces mitotic slippage and genome reduplication in HCT116. Cells were treated with the indicated concentrations of Barasertib for 24 h. DNA contents were analyzed with flow cytometry. (f) Cytotoxicity induced by Barasertib. HeLa and HCT116 cells were cultured in the presence of the indicated concentrations of Barasertib for 48 h. Proliferation was assayed with WST-1 assay. (g) Barasertib induces genome reduplication and apoptosis. HeLa cells were incubated with 50 n M of Barasertib either in the presence or absence of the caspase inhibitor Z-VAD(OMe)-FMK. The cells were harvested at the indicated time points and analyzed with flow cytometry.

    Oncogene 2014 33, 3550-60. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

  •  

    Dual inhibition of Aurora and SRC kinases specifically eliminates hyperploid cells. Experiment shown is same as a, b, but performed following treatment of OVCAR10 cells with MLN8237 (targeting AURKA) or AZD1152 (targeting AURKB);

    Oncogene 2012 31, 1217–1227. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

    D, induction of aneuploidy was repressed by AKT3. Active AKT3 mutant, either myr-AKT3 or AKT3 (E17K), was transiently expressed either in HCT 116, MCF7, or OVCAR3 cells. The cells were then treated with AZD1152-HQPA for 2 days. Control cells were not treated with the drug, and nocodazole (100 nM for 24 h)-treated HCT 116 cell nuclei were also shown as reference for G2-arrested cells. After fixation, nuclei were stained with DAPI (blue signal) and AKT3-expressing cells were detected with anti-HA staining (red signal). Confocal microscopic analysis was performed, and representative images are shown.

    J Biol Chem, 2017, 292(5):1910-1924. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

  • p53 phosphorylation by Aurora B. A, p53 reporter construct was co-transfected with the indicated plasmids into H1299 cells and reporter activation was determined as described under "Experimental Procedures". B, U2OS cells and H1299 cells were treated with AZD1152 (AZD) for 12 h at the indicated doses. Cell lysates were harvested and immunoblotted with Bax and actin antibodies. C, U2OS cells were treated with 100 ng/ml nocodazole (noc) overnight, and then shake off cells were harvested, washed with PBS, and reseeded. Approximately 2 h later, cells were either lysated or treated with dimethyl sulfoxide (DMSO) or AZD1152 for another 16 h before harvesting. Cell lysates were immunoblotted with Bax, phospho-H3, and actin antibodies. D, GST-p53 or GST control proteins were incubated with Aurora B protein and analyzed for phosphate incorporation (left panel). Coomassie staining of GSTp53 and GST protein is also shown (right panel). E, In vitro phosphorylation sites of GST-p53 identified by mass spectrometry analysis. F, GST-p53 wild-type and 3A mutant proteins were analyzed in a kinase assay as in B. G, plasmids encoding wild-type or 3A mutant (CMV)-FLAG-p53 were transiently transfected into H1299 cells, with or without Myc-Aurora B (AurB) expression vector. 20 h post-transfection, cells were lysed and subjected to immunoprecipitation (IP) with p53 antibody (fl-393). Precipitates were immunoblotted with antibodies to p53 (DO-1), Thr(P) and Ser(P), as indicated. Vec, vector.

     

     

    J Biol Chem 2011 286, 2236-44. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

    Fig. 5.A, inhibition of VEGF-mediated uterine edema. Compounds were administered intravenously at the indicated dose 30 min before estradiol challenge. Uterine edema was assessed 2 h thereafter. Inhibition > 35% of the response was significantly different from vehicle-treated group (P < 0.05). ED50(milligrams per kilogram) is shown within parentheses. Values are expressed as mean  S.E.M., n= 6 per group. IV, intravenously. B, induction of plasma PLGF after treatment with ABT-348. Mice-bearing tumors derived from a human NSCLC cell line (HCC827ER) were treated with 25 mg/kg ABT-348 via subcutaneous osmotic minipump. At the indicated time, plasma samples were obtained and assayed for murine PLGF. Values shown are the mean  S.E. (n = 5 per group). C, representative longitudinal MRI images showing gadolinium contrast enhancement in a rat glioma model with treatment with vehicle, ABT-348 (6.25 mg/kg i.p. b.i.d., every 7 days; two treatment cycles on days 11 and 18 after inoculation), or AZD1152(25 mg/kg i.v., every 4 days; two treatment cycles commencing on days 11 and 18 after inoculation). b, normal brain; t, tumor, Tx1, first treatment cycl e; Tx2, second treatment cycle. D,K transas a function of treatment cycle. Values represent the mean  S.E.M., n =12 per group.**, P < 0.01 vs. vehicle.

    J Pharmacol Exp Ther 2012 343(3), 617-27. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

  • Effects of vitamin C and K3 (Vit. C&K3; 300 μM and 1 μM, respectively), barasertib (0.01 μM) and their combination on cell viability, induction of apoptosis, level of reactive oxygen species (ROS) and level of protein-carbonyl products in Jurkat leukemia cells (A) and normal lymphocytes (B), after 24- and 48-h incubation at 37˚C in humidified atmosphere. The data are the mean±SD from three independent experiments.

    Anticancer Res, 2018, 38(3):1407-1414. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

    1205Lu cells were treated for 48hours with the indicated concentrations of  AZD1152-HQPA. 

     

     

    Dr. Gao Zhang of University of Pennsylvania. Barasertib (AZD1152-HQPA|AZD2811) purchased from Selleck.

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description Barasertib (AZD1152-HQPA) is a highly selective Aurora B inhibitor with IC50 of 0.37 nM in a cell-free assay, ~3700 fold more selective for Aurora B over Aurora A. Phase 1.
Targets
Aurora B [1]
(Cell-free assay)
0.37 nM
In vitro

AZD1152 displays >3000-fold selectivity for Aurora B as compared with Aurora A which has an IC50 of 1.368 μM. AZD1152 has even less activity against 50 other serine-threonine and tyrosine kinases including FLT3, JAK2, and Abl. AZD1152 inhibits the proliferation of hematopoietic malignant cells such as HL-60, NB4, MOLM13, PALL-1, PALL-2, MV4-11, EOL-1, THP-1, and K562 cells with IC50 of 3-40 nM, displaying ~100-fold potency than another Aurora kinase inhibitor ZM334739 which has IC50 of 3-30 μM. AZD1152 inhibits the clonogenic growth of MOLM13 and MV4-11 cells with IC50 of 1 nM and 2.8 nM, respectively, as well as the freshly isolated imatinib-resistant leukemia cells with IC50 values of 1-3 nM, more significantly compared with bone marrow mononuclear cells with IC50 values of >10 nM. AZD1152 induces accumulation of cells with 4N/8N DNA content, followed by apoptosis in a dose- and time-dependent manner. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LNCaP Mon1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm[5NE02ODBibl2= MUW0POKhcA>? M3HiOWlEPTB;MkWgcm0> M4LnfFI2Ojd5NkW5
LNCaP MnfzRZBweHSxc3nzJGF{e2G7 NHLnbZIxNTVyMDDuUS=> MVy0POKhcA>? MXfpcoR2[2W|IHHwc5B1d3SrYzDj[YxtKGSnYYToJJRpem:3Z3igZ4F{eGG|ZT2zJJVxemWpdXzheIlwdg>? NUjXTpl6OjV{N{e2OVk>
LNCaP NVfMW3pRTnWwY4Tpc44hSXO|YYm= NWH2PZlbPTBibl2= NXnsO21rPDhiaB?= MorZbY5lfWOnczDtbYNzd263Y3zlbUB4cXSqIHHu[ZVo\W6rYzDt[YNp[W6rc32= Ml\DNlUzPzd4NUm=
Ramos NXXpdIl6TnWwY4Tpc44hSXO|YYm= NGTPR3c2ODBibl2= NGTPNmIxNTd{IHi= MV3pcohq[mm2czDBeZJwemFiQjDrbY5ie2V? NXrRT3BVOjF|N{G0OFY>
Daudi  M3\tO2Z2dmO2aX;uJGF{e2G7 MUe1NFAhdk1? M3jm[|AuPzJiaB?= MUjpcohq[mm2czDBeZJwemFiQjDrbY5ie2V? MVSyNVM4OTR2Nh?=
L540 NH3UO3BHfW6ldHnvckBCe3OjeR?= MVy1NFAhdk1? NFqyS5YxNTd{IHi= NIfFXpFqdmirYnn0d{BCfXKxcnGgRkBscW6jc3W= NWDLV2xNOjF|N{G0OFY>
BJAJ NEnaW2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mof1OVAxKG6P NUjCfWFoOC15MjDo NGTXZ|FqdmirYnn0d{Bk\WyuIHfyc5d1cCC|aXfubYZq[2GwdHz5 NWDuVJZSOjF|N{G0OFY>
Ramos M1;SRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUm1NFAhdk1? NHzTXY4xNTd{IHi= NYKzSXhicW6qaXLpeJMh[2WubDDndo94fGhic3nncolncWOjboTsfS=> M37ucFIyOzdzNES2
Raji NF3TcoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV61NFAhdk1? M2n1TVAuPzJiaB?= MVfpcohq[mm2czDj[YxtKGe{b4f0bEB{cWewaX\pZ4FvfGy7 NYP5fItEOjF|N{G0OFY>
Daudi  M2rUS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXP[pY5PTByIH7N MWewMVczKGh? NHHieZJqdmirYnn0d{Bk\WyuIHfyc5d1cCC|aXfubYZq[2GwdHz5 MkTCNlE{PzF2NE[=
L428 NWLtb3RQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX:zNWgzPTByIH7N NXHid5JzOC15MjDo NVTqUGFKcW6qaXLpeJMh[2WubDDndo94fGh? NX7tXZB1OjF|N{G0OFY>
KM-H2 Mn;US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HwelUxOCCwTR?= NXe0XYhTOC15MjDo NFP2fJJqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M4X5W|IyOzdzNES2
HDLM-2 NWe4N4hPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;YTXk2ODBibl2= NVLhdWRwOC15MjDo MmnlbY5pcWKrdIOgZ4VtdCCpcn;3eIg> MUKyNVM4OTR2Nh?=
L450 MlHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PLfVUxOCCwTR?= MYSwMVczKGh? M4\ENIlvcGmkaYTzJINmdGxiZ4Lve5Rp M3yze|IyOzdzNES2
BJAJ MXLBdI9xfG:|aYOgRZN{[Xl? NI\aNHY2ODBibl2= M1iyPFAuPzJiaB?= NUPYRok1cW6mdXPld{BieG:ydH;zbZMhcW5iYTD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MVyyNVM4OTR2Nh?=
Ramos NXyyN2J2SXCxcITvd4l{KEG|c3H5 MUO1NFAhdk1? MUGwMVczKGh? NEXFVlFqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NF7mTGEzOTN5MUS0Oi=>
Raji M4nzemFxd3C2b4Ppd{BCe3OjeR?= NUDlTHRCPTByIH7N NHvEUmsxNTd{IHi= NF\JOmpqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MUKyNVM4OTR2Nh?=
Daudi  NHLyZ3hCeG:ydH;zbZMhSXO|YYm= NUHBPVk1PTByIH7N MYSwMVczKGh? NWTpWXVlcW6mdXPld{BieG:ydH;zbZMhcW5iYTD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MVSyNVM4OTR2Nh?=
L428 MYPBdI9xfG:|aYOgRZN{[Xl? MWi1NFAhdk1? MV6wMVczKGh? M3:4VIlv\HWlZYOgZZBweHSxc3nzJIlvKGFidHnt[U1l\XCnbnTlcpQhdWGwbnXy NHWycVYzOTN5MUS0Oi=>
KM-H2 MYXBdI9xfG:|aYOgRZN{[Xl? NUW1ZZNVPTByIH7N NYfBeJQzOC15MjDo MX\pcoR2[2W|IHHwc5B1d3OrczDpckBiKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MV6yNVM4OTR2Nh?=
HDLM-2 NEHQc5ZCeG:ydH;zbZMhSXO|YYm= MUm1NFAhdk1? M2fGe|AuPzJiaB?= NFPB[ZRqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M1PsOFIyOzdzNES2
L450 M2K2SWFxd3C2b4Ppd{BCe3OjeR?= M4TFZlUxOCCwTR?= MXewMVczKGh? M3nW[Ilv\HWlZYOgZZBweHSxc3nzJIlvKGFidHnt[U1l\XCnbnTlcpQhdWGwbnXy Ml2yNlE{PzF2NE[=
SW620 NXPaR5ppT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHywPYFGSzVyPUGwxtEzNjFibl2= Mn70NlEzPDVyOUC=
HCT116 NGnQWFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13YemVEPTB;MUJCtVMvOyCwTR?= NHS3cVUzOTJ2NUC5NC=>
MDA-MB-435 NIfoWYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoSxNE0yODByMDDuUS=> NHfLXJMzNTViZB?= M2rwOGROW09? MXLJR|UxRTF{NTDuUS=> MWmyNFE4PTl{Nh?=
MDA-MB-468 M4W0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Kye|AuOTByMECgcm0> Mm\1Nk02KGR? NXfxZXU6TE2VTx?= NYrTdY1YUUN3ME2xOEBvVQ>? MnfJNlAyPzV7Mk[=
MDA-MB-231 M3XTUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrzNIxbOC1zMECwNEBvVQ>? NWrIcmptOi13IHS= NH\kUoVFVVOR NGfnXo5KSzVyPUGwOUBvVQ>? NXi5UJR5OjBzN{W5NlY>
BT474 NXHFXnZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVGwMVExODByIH7N NUK2eoY2Oi13IHS= NWnXb2dpTE2VTx?= M1zSTGlEPTB;ODDuUS=> M1Px[VIxOTd3OUK2
MDA-MB-361 NYHndIh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW[wMVExODByIH7N NIHONYQzNTViZB?= MmrqSG1UVw>? NYXOem5iUUN3ME23NEBvVQ>? Mnm4NlAyPzV7Mk[=
HER18 NF7WWnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHBNE0yODByMDDuUS=> NE\2e3gzNTViZB?= M1vSS2ROW09? MnTJTWM2OD1{MDDuUS=> NHziRYIzODF5NUmyOi=>
HER18 M2nJRWFxd3C2b4Ppd{BCe3OjeR?= NWX6SVdTOTByIH7N MnizNE8zPC92ODDo NGjLe4VFVVOR MXTpcoR2[2W|IHHwc5B1d3OrczDhcoQhemWmdXPld{BkdG:wb3flcolkKHCxdHXueIlidA>? M3;LPVIxOTd3OUK2
MDA-MB-231 MW\BdI9xfG:|aYOgRZN{[Xl? M2nq[FExPSCwTR?= Ml3JNE8zPC92ODDo M3jzUWROW09? NUPKbml{cW6mdXPld{BieG:ydH;zbZMh[W6mIILl[JVk\XNiY3zvco9o\W6rYzDwc5RmdnSrYXy= MU[yNFE4PTl{Nh?=
JHH-1 MnKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnr0NE4{6oDVMUCwNOKhdk1? NVfwTW1OPzJiaB?= MkDjSWM2OD1zNz60xtEyNjBibl2= MVyxPVkyOzl|NR?=
JHH-2 Mk\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTVZYYxNjQkgKOxNFAxyqCwTR?= MlywO|IhcA>? MYDFR|UxRTJzOD6wxtEyOC56IH7N NHLJb2wyQTlzM{mzOS=>
JHH-4 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoK1NE4{6oDVMUCwNOKhdk1? NULxZoZRPzJiaB?= NUDTTWZiTUN3ME2xOVUvPsLzMU[uPEBvVQ>? MUGxPVkyOzl|NR?=
HuH-1 NFHwO4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPoN4sxNjQkgKOxNFAxyqCwTR?= MkjlO|IhcA>? NV3LT3ptTUN3ME2yO{4{yrF3LkCgcm0> MVmxPVkyOzl|NR?=
HuH-6 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYiwMlPjiJNzMECwxsBvVQ>? NIXkc4g4OiCq M1fWWGVEPTB;Mz63xtExNjZibl2= M3rxPFE6QTF|OUO1
HuH-7 MmS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\YcGxiOC5|4pETNVAxOMLibl2= MUm3NkBp NUT3O2J2TUN3ME22MljDuTBwMzDuUS=> NXXpNZRPOTl7MUO5N|U>
HLE M{HvNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrvWJUxNjQkgKOxNFAxyqCwTR?= MmLtO|IhcA>? MXrFR|UxRTR3LkpCtVYvPCCwTR?= NIjqZ4YyQTlzM{mzOS=>
HLF MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUCwMlPjiJNzMECwxsBvVQ>? NUTWNoFiPzJiaB?= M1;V[GVEPTB;MUK2MlHDuTF{LkKgcm0> M3LBS|E6QTF|OUO1
PLC/PRF/5 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYewMlPjiJNzMECwxsBvVQ>? NYPPVGVtPzJiaB?= NVrMdndlTUN3ME23Ok46yrF7Lkmgcm0> MWixPVkyOzl|NR?=
SK-Hep1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mli5NE4{6oDVMUCwNOKhdk1? Ml\1O|IhcA>? NIj5WJVGSzVyPUKxMlnDuTFwMjDuUS=> M{fvUFE6QTF|OUO1
Hep3B NHPWUpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3kbZQxNjQkgKOxNFAxyqCwTR?= NEHBXHo4OiCq MV\FR|UxRTdwNtMxNU4zKG6P NHL3TFcyQTlzM{mzOS=>
HepG2 MnfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVWwMlPjiJNzMECwxsBvVQ>? Mn7sO|IhcA>? MmLySWM2OD1zND63xtEyNjdibl2= NHLmOXgyQTlzM{mzOS=>
Ramos MlPhRZBweHSxc3nzJGF{e2G7 M3jYVVI2NzVyL{GwNEBvVQ>? NEjsUWY1QCCq M3[wcIlv[3KnYYPld{B1cGVibHX2[Yx{KG:oIITo[UBkdGWjdnXkJIZwem2|IH;mJHBCWlBiYX7kJINie3Cjc3WgNy=> M1nF[FE6QDJ|MU[4
Daudi  M{\4UWFxd3C2b4Ppd{BCe3OjeR?= MoO2NlUwPTBxMUCwJI5O NUfhXGNsPDhiaB?= M2TZOIlv[3KnYYPld{B1cGVibHX2[Yx{KG:oIITo[UBkdGWjdnXkJIZwem2|IH;mJHBCWlBiYX7kJINie3Cjc3WgNy=> NUfZ[G5COTl6MkOxOlg>
BALM-14 MWnBdI9xfG:|aYOgRZN{[Xl? NFS2ToYyOi53L{K1M|UxKG6P NVfzPXB4PDhiaB?= M4G0Xolv[3KnYYPld{B1cGVibHX2[Yx{KG:oIITo[UBkdGWjdnXkJIZwem2|IH;mJHBCWlBiYX7kJINie3Cjc3WgNy=> MonuNVk5OjNzNki=
BALM-27 NV7rZ|BPSXCxcITvd4l{KEG|c3H5 NF[5[pYyOi53L{K1M|UxKG6P MmnXOFghcA>? M1vNfIlv[3KnYYPld{B1cGVibHX2[Yx{KG:oIITo[UBkdGWjdnXkJIZwem2|IH;mJHBCWlBiYX7kJINie3Cjc3WgNy=> M{PsOFE6QDJ|MU[4
NB4 Mn;tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXSwMlAyNzBwMT:xJO69VQ>? NHHafVA1QCCq MnS0bY5pcWKrdIOgZ4VtdCCpcn;3eIghe2mpbnnmbYNidnSueR?= M4\1bFE5OzZ5NEi0

... Click to View More Cell Line Experimental Data

In vivo Administration of AZD1152 (25 mg/kg) alone markedly suppresses the growth of MOLM13 xenografts, confirmed by the observation of necrotic tissue with infiltration of phagocytic cells. [1] In addition, AZD1152 (10-150 mg/kg/day) significantly inhibits the growth of a variety of human solid tumor xenografts, including colon, breast, and lung cancers, in a dose-dependent manner. [2]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: HL-60, NB4, MOLM13, PALL-2, MV4-11, EOL-1, and K562 cells
  • Concentrations: Dissolved in DMSO, final concentrations ~100 nM
  • Incubation Time: 24 or 48 hours
  • Method: Cells are exposed to various concentrations of AZD1152 for 24 or 48 hours. Cell proliferation is measured by 3H-thymidine uptake (isotope added 6 hours before harvest), and the concentration that induced 50% growth inhibition (IC50) is calculated from dose-response curves. Cell cycle analysis is performed by flow cytometry. Cell apoptosis is measured by annexin V–FITC apoptosis detection kit.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Female immune-deficient BALB/c nude mice subcutaneously injected with MOLM13 cells
  • Formulation: Dissolved in 3M Tris, pH 9.0, at a concentration of 2.5 mg/mL
  • Dosages: 5 or 25 mg/kg
  • Administration: Intraperitoneal injection 4 times a week or every another day
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 102 mg/mL (200.96 mM)
Ethanol 3 mg/mL (5.91 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.
7mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 507.56
Formula

C26H30FN7O3

CAS No. 722544-51-6
Storage powder
in solvent
Synonyms INH 34

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03366675 Terminated Small Cell Lung Cancer Samsung Medical Center|AstraZeneca December 1 2017 Phase 2
NCT03366675 Terminated Small Cell Lung Cancer Samsung Medical Center|AstraZeneca December 1 2017 Phase 2
NCT03217838 Recruiting Acute Myeloid Leukaemia AstraZeneca July 31 2017 Phase 1|Phase 2
NCT03217838 Recruiting Acute Myeloid Leukaemia AstraZeneca July 31 2017 Phase 1|Phase 2
NCT02579226 Recruiting Advanced Solid Tumours AstraZeneca October 28 2015 Phase 1
NCT02579226 Recruiting Advanced Solid Tumours AstraZeneca October 28 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Can you let me know what solvent I can use for Barasertib, cat # S1147, for in vivo use? (IP injection in mice)

  • Answer:

    S1147 Barasertib (AZD1152-HQPA) can be dissolved in 30% PEG400/0.5% Tween80/5% Propylene glycol at 30mg/ml as a clear solution. Usually, when prepare the solution, we will add organic solvents first, then add Tween 80, then water. But this compound can not dissolve in 30% PEG400/0.5% Tween80/5% Propylene glycol clearly. After water was added, it became a clear solution.

Aurora Kinase Signaling Pathway Map

Aurora Kinase Inhibitors with Unique Features

Related Aurora Kinase Products5

Tags: buy Barasertib (AZD1152-HQPA|AZD2811) | Barasertib (AZD1152-HQPA|AZD2811) supplier | purchase Barasertib (AZD1152-HQPA|AZD2811) | Barasertib (AZD1152-HQPA|AZD2811) cost | Barasertib (AZD1152-HQPA|AZD2811) manufacturer | order Barasertib (AZD1152-HQPA|AZD2811) | Barasertib (AZD1152-HQPA|AZD2811) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID