ZM 447439

For research use only. Not for use in humans.

Catalog No.S1103

24 publications

ZM 447439 Chemical Structure

Molecular Weight(MW): 513.59

ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.

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Selleck's ZM 447439 has been cited by 24 publications

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.
Features An Aurora selective ATP-competitive inhibitor.
Targets
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
In vitro

In vitro, ZM-447439 selectively inhibits recombinant human Aurora A and B with IC50 values of 110 and 130 nM, respectively, while other protein kinases of diverse structural types including the mitotic kinases CDK1 and PLK1 are inhibited with IC50 values >10 μM. [1] Aurora kinase inhibitor, ZM-447439 time- and dose-dependently inhibits the growth of all three cell lines with IC50 values of 3 μM (BON), 0.9 μM (QGP-1) and 3 μM (MIP-101) after 72 hours of continuous exposure. In addition, ZM-447439 potently induces cell apoptosis by promoting DNA fragmentation and caspase 3 and 7 activation, and arrests GEP-NET cells in the G0 /G1and G2/M phase of the cell cycle. [2] In mouse embryo, inhibition of Aurora kinase activity by ZM-447439 results in abnormalities during mitosis by regulating the phosphorylation of histone H3 serine 10 (H3S10Ph) from G2 to metaphase with different perturbations in each embryonic cycle. [3] A recent study shows that ZM-447439 exhibits growth inhibitory and proapoptotic effect on cervical cancer SiHa cells, and enhances the chemosensitivity to cisplatin. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF21XFhKdmirYnn0bY9vKG:oIHj1cYFvKEWxTD2xMYNmdGxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG4Olc5KM7:TR?= MnHTV2FPT0WU
MCF7 cell MYfQdo9tcW[ncnH0bY9vKGG|c3H5 MnzBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNR2Y4KGOnbHygcIlv\SxiSVO1NF0xNjF7ODFOwG0> MVSxOlM{PzF{Mh?=
human P12-ICHIKAWA cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Moq1TY5pcWKrdHnvckBw\iCqdX3hckBROTJvSVPITWtCX0FiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkKyOFQyKM7:TR?= M1zUN3NCVkeHUh?=
human KARPAS-45 cell M2HldWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmrsTY5pcWKrdHnvckBw\iCqdX3hckBMSVKSQWOtOFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN6MUK4JO69VQ>? NXXGWndQW0GQR1XS
human ES3 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NU\IWm5CUW6qaXLpeIlwdiCxZjDoeY1idiCHU{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ4OzJizszN M2PyVnNCVkeHUh?=
human ES8 cell M3vrNmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{fzfGlvcGmkaYTpc44hd2ZiaIXtZY4hTVN6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD60PVgxPiEQvF2= M3mwb3NCVkeHUh?=
human TE-11 cell NFfFWYhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHvmUnRKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42OzdyMzFOwG0> Ml23V2FPT0WU
human RS4-11 cell Mm\vS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULoNJBZUW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjV3NESg{txO Mli5V2FPT0WU
human MOLT-16 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoXNTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43ODl4MTFOwG0> MVXTRW5ITVJ?
human RKO cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGfae4FKdmirYnn0bY9vKG:oIHj1cYFvKFKNTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|A3PTZizszN MkjjV2FPT0WU
human MV-4-11 cell M1fD[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVvJcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlc6PjNizszN MmDxV2FPT0WU
human SW954 cell NYD2OGZNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoTXTY5pcWKrdHnvckBw\iCqdX3hckBUXzl3NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFM3OzVizszN NVT3XHZZW0GQR1XS
human BE-13 cell NIHzcGVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVnJcohq[mm2aX;uJI9nKGi3bXHuJGJGNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD64OFQyQCEQvF2= NFi5doNUSU6JRWK=
human MOLT-4 cell MkHmS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlvGTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg6QTd6IN88US=> MkjNV2FPT0WU
human NBsusSR cell M3SxW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4rSbGlvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQTF|OEeg{txO NGLxO|JUSU6JRWK=
human H9 cell M1HCSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn;PTY5pcWKrdHnvckBw\iCqdX3hckBJQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwOUK5O|kh|ryP MlvTV2FPT0WU
human A172 cell M3PLOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jkNGlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVg1OTFizszN M4XlZnNCVkeHUh?=
human ES5 cell Mni2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVvJcohq[mm2aX;uJI9nKGi3bXHuJGVUPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMECyOFgh|ryP M{j0NHNCVkeHUh?=
human SBC-1 cell M3m0SWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXf1PYp7UW6qaXLpeIlwdiCxZjDoeY1idiCVQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODN7Mkig{txO M2PNNHNCVkeHUh?=
human NCI-H209 cell MkjhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3HIWmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF4NkCyJO69VQ>? M1vz[nNCVkeHUh?=
human NKM-1 cell NIX3co9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYPVNYYyUW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTZ5OUig{txO NWKxVplPW0GQR1XS
human NCI-H720 cell MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYTOU5Q3UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFczOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMkC2Nlch|ryP M1:yU3NCVkeHUh?=
human KE-37 cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3q5WWlvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIyOzh6IN88US=> NEf6bW5USU6JRWK=
human SW48 cell M3TVUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVzPSnVUUW6qaXLpeIlwdiCxZjDoeY1idiCVV{S4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zOzF3NTFOwG0> NIjCfXdUSU6JRWK=
human IST-SL1 cell NF3kc3BIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXG3VZZjUW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5|MUeyO{DPxE1? Ml7MV2FPT0WU
human SK-NEP-1 cell MmmzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI\yT5dKdmirYnn0bY9vKG:oIHj1cYFvKFONLV7FVE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5|NkS5PEDPxE1? MUHTRW5ITVJ?
human NOMO-1 cell NHTTW4VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXPXXHBYUW6qaXLpeIlwdiCxZjDoeY1idiCQT13PMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4N{K1JO69VQ>? MXnTRW5ITVJ?
human DOHH-2 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOFAzPzZizszN MkjpV2FPT0WU
human ABC-1 cell NWfrfnN1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGFDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT60NFM2OiEQvF2= M173fnNCVkeHUh?=
human Ramos-2G6-4C10 cell NHLaVm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGK5fG5KdmirYnn0bY9vKG:oIHj1cYFvKFKjbX;zMVJIPi12Q{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODZyNTFOwG0> MnzCV2FPT0WU
human EM-2 cell MkSwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn;0TY5pcWKrdHnvckBw\iCqdX3hckBGVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT60NVczOSEQvF2= NIDHU3RUSU6JRWK=
human NB14 cell MkDES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHPRcXVKdmirYnn0bY9vKG:oIHj1cYFvKE6EMUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlU2PjJzIN88US=> MlP0V2FPT0WU
human MOLT-13 cell NV\JcWxJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVPJVWF3UW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVE{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53NkewOkDPxE1? NGfzPHRUSU6JRWK=
human ECC10 cell MnHpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmnqTY5pcWKrdHnvckBw\iCqdX3hckBGS0NzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlM{PTNizszN NXv1enI{W0GQR1XS
human LK-2 cell NX25S3dDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXPISXRvUW6qaXLpeIlwdiCxZjDoeY1idiCOSz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43PDV7NDFOwG0> M2HwbXNCVkeHUh?=
human CTB-1 cell MlXjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnvmTY5pcWKrdHnvckBw\iCqdX3hckBEXEJvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlcxQDJizszN M1zPU3NCVkeHUh?=
human NCI-H1581 cell M1nYOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{LpPGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixOVgyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS54N{W1JO69VQ>? M1XHTnNCVkeHUh?=
human COLO-800 cell NEPsR4NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYXJcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vOECwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU44ODN6MjFOwG0> MlXPV2FPT0WU
human NB7 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVnJR3dNUW6qaXLpeIlwdiCxZjDoeY1idiCQQkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlc2Ojl5IN88US=> MnjTV2FPT0WU
human LAMA-84 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NELwe2ZKdmirYnn0bY9vKG:oIHj1cYFvKEyDTVGtPFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjd3NUKg{txO MVTTRW5ITVJ?
human HCT-116 cells M4njT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2iwemlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDB7MEig{txO MmrvV2FPT0WU
SK-UT-1 cell MkXHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFfuS5JKdmirYnn0bY9vKG:oIHj1cYFvKFONLWXUMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjhzNUOg{txO Mm\GV2FPT0WU
human H4 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknuTY5pcWKrdHnvckBw\iCqdX3hckBJPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwOEG1O|gh|ryP Mo\wV2FPT0WU
human CAL-51 cell MlL3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPFM5PDVizszN Mkn2V2FPT0WU
human LoVo cells Ml\4VJJwdGmoZYLheIlwdiCjc4PhfS=> NUjzUpQ5PzJiaB?= M1LnVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTH;Wc{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUMYJie2WmIGfTWFghemWjZ3XueEBie3OjeTygTWM2OD1zLkmg{txO MoHaNlUzPzB2MEO=
human HN cell MonLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M161eGlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkmyOVEh|ryP NYDuVYF1W0GQR1XS
human L-363 cell NI[1XmVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHP2O|lKdmirYnn0bY9vKG:oIHj1cYFvKExvM{[zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU46PTF{IN88US=> MVHTRW5ITVJ?
human NCI-H747 cell NEG4S4RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVrJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KN{S3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4xOzN3MzFOwG0> NUjmNFQ5W0GQR1XS
human A498 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVrPVphQUW6qaXLpeIlwdiCxZjDoeY1idiCDNEm4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4{PjZ7IN88US=> NGHreVRUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p21 / p-p38 / p38; 

PubMed: 23759594     


U2OS cells were treated for the indicated time with 1 µM ZM447439. Levels of p53, p21, phosphorylated p38 (p-p38) and total p38 were determined by immunoblotting.

pHistone H3 / CEM ; 

PubMed: 22359551     


Detection of phospho Histone H3(Ser10) in CEM and CEM/AKB4 cells treated for 24 hr with increasing concentrations of ZM447439 by western blotting. Shown are representative blots from three independent experiments.

BubR1 / MAD2 / Cyclin B1 ; 

PubMed: 26188358     


ZM induces mitotic slippage in AML cells. NB4 cells were incubated with different doses of ZM (0.5, 1, 2, 5 and 10 μM) or DMSO for 48h; Whole-cell extracts was subjected to immunoblotting by using indicated antibodies.

23759594 22359551 26188358
Immunofluorescence
p21; 

PubMed: 23759594     


U2OS cells were treated with 1 µM ZM447439 for 24 h. Nuclei were stained with Hoechst 33258. p21 was detected by immunostaining. Bar: 50 µm. 

p53; 

PubMed: 23759594     


U2OS cells were treated for the indicated time with 1 µM ZM447439. Levels of p53, p21, phosphorylated p38 (p-p38) and total p38 were determined by immunoblotting.

Aurora B / Survivin ; 

PubMed: 12719470     


Immunofluorescence images of prometaphase DLD-1 cells stained to detect Aurora B (green), Survivin (red), and DNA (blue) after exposure to ZM447439 for 1 h.

23759594 12719470

Protocol

Kinase Assay:[1]
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In vitro kinase assays :

Recombinant Aurora A and B are expressed as NH2-terminal His6-tagged fusion proteins using a baculovirus expression system. Aurora A is purified by affinity chromatography using Ni-NTA agarose, and Aurora B is purified by ion exchange chromatography using CM Sepharose Fast Flow. 1 ng purified recombinant enzyme is added to a reaction cocktail containing 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM peptide substrate, 10 μM for Aurora A or 5 μM ATP for Aurora B, and 0.2 μCi γ-[33P]ATP (specific activity ≥2,500 Ci/mmol), and is then incubated at RT for 60 minutes. Reactions are stopped by addition of 20% phosphoric acid, and the products are captured on P30 nitrocellulose filters and assayed for incorporation of 33P with a BetaplateTM counter. No enzyme and no compound control values are used to determine the concentration of ZM447439, which gave 50% inhibition of enzyme activity. Further details are available on request from Nicholas Keen.
Cell Research:[2]
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  • Cell lines: BON, QGP-1 and MIP-101 cells
  • Concentrations: 0-5 μM
  • Incubation Time: 72 hours
  • Method: Cell number is evaluated by crystal violet staining. In brief, cells in 96-well plates are fixed with 1% glutaraldehyde. Then cells are stained with 0.1% crystal violet. The unbound dye is removed by washing with water. Bound crystal violet is solubilized with 0.2% Triton X-100. Light extinction which increases linearly with the cell number is analyzed at 570 nm using an ELISA reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.59
Formula

C29H31N5O4

CAS No. 331771-20-1
Storage powder
in solvent
Synonyms N/A
Smiles COC1=C(OCCCN2CCOCC2)C=C3N=CN=C(NC4=CC=C(NC(=O)C5=CC=CC=C5)C=C4)C3=C1

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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