ZM 447439

Catalog No.S1103

ZM 447439 Chemical Structure

Molecular Weight(MW): 513.59

ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.

Size Price Stock Quantity  
In DMSO USD 98 In stock
USD 70 In stock
USD 110 In stock
USD 270 In stock
USD 470 In stock
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3 Customer Reviews

  • p31comet depletion delays MCC disassembly even with the proteasome inhibited. (A) FACS analysis of HeLa Tet-on cells transfected with control or p31comet siRNA and then treated with Taxol followed by Aurora B inhibition with ZM447439. The percentage of mitotic cells (cells that have 4N DNA content and are MPM2 positive) is shown for each sample.

    Mol Biol Cell 2011 22, 4227-35. ZM 447439 purchased from Selleck.

    HeLa cells were treated with Nocodazole (100ng/ml) (Lanes 2-4) or Taxol (1µM) (Lanes 6-8) or DMSO (lanes 1, 5) for 16h. The indicated inhibitors were added for 2h (1 µM) before harvesting the cells. The p-Aurora-A (T288), B (T232), C (T198) antibody was from Cell Signaling (#2914). MK5108 is an Aurora-A inhibitor. VX680 inhibits all three Aurora kinases. ZM447439 inhibits both Aurora-B and -C kinases, but not Aurora-A kinase.

    Dr. Yuanhong Chen of University of Nebraska. ZM 447439 purchased from Selleck.

  • Western blot analysis of Histone and Aurora kinase. 0-10μM ZM447439 was added.

    Dr. Zhang of Tianjin Medical University. ZM 447439 purchased from Selleck.

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.
Features An Aurora selective ATP-competitive inhibitor.
Targets
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
In vitro

In vitro, ZM-447439 selectively inhibits recombinant human Aurora A and B with IC50 values of 110 and 130 nM, respectively, while other protein kinases of diverse structural types including the mitotic kinases CDK1 and PLK1 are inhibited with IC50 values >10 μM. [1] Aurora kinase inhibitor, ZM-447439 time- and dose-dependently inhibits the growth of all three cell lines with IC50 values of 3 μM (BON), 0.9 μM (QGP-1) and 3 μM (MIP-101) after 72 hours of continuous exposure. In addition, ZM-447439 potently induces cell apoptosis by promoting DNA fragmentation and caspase 3 and 7 activation, and arrests GEP-NET cells in the G0 /G1and G2/M phase of the cell cycle. [2] In mouse embryo, inhibition of Aurora kinase activity by ZM-447439 results in abnormalities during mitosis by regulating the phosphorylation of histone H3 serine 10 (H3S10Ph) from G2 to metaphase with different perturbations in each embryonic cycle. [3] A recent study shows that ZM-447439 exhibits growth inhibitory and proapoptotic effect on cervical cancer SiHa cells, and enhances the chemosensitivity to cisplatin. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell NYTHNnZUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkSyTY5pcWKrdHnvckBw\iCqdX3hckBGd0xvMT3j[YxtKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zOE[3PEDPxE1? M3zpd3NCVkeHUh?=
MCF7 cell Ml\VVJJwdGmoZYLheIlwdiCjc4PhfS=> M3W5[mFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUWNHPyClZXzsJIxqdmVuIFnDOVA:OC5zOUig{txO NF75S2kyPjN|N{GyNi=>
human P12-ICHIKAWA cell M3;6Umdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2[2XWlvcGmkaYTpc44hd2ZiaIXtZY4hWDF{LVnDTGlMSVeDIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlQ1OSEQvF2= MVPTRW5ITVJ?
human KARPAS-45 cell NFHFWXZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{TEUmlvcGmkaYTpc44hd2ZiaIXtZY4hU0GUUFHTMVQ2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|OEGyPEDPxE1? NYn3Rmx4W0GQR1XS
human ES3 cell MkHLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXnJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNEezNkDPxE1? NXzwPI5ZW0GQR1XS
human ES8 cell M1zuTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX7TPGJwUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ6QDB4IN88US=> NHLhOHFUSU6JRWK=
human TE-11 cell M4HM[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYrQWIhtUW6qaXLpeIlwdiCxZjDoeY1idiCWRT2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPTN5MEOg{txO M3:0d3NCVkeHUh?=
human RS4-11 cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXK4SGtUUW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjV3NESg{txO NVy2TlZDW0GQR1XS
human MOLT-16 cell M3\qUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MojOTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE43ODl4MTFOwG0> M{jZdXNCVkeHUh?=
human RKO cell NXy4fohqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnzDTY5pcWKrdHnvckBw\iCqdX3hckBTU09iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkewOlU3KM7:TR?= NXn6XVNkW0GQR1XS
human MV-4-11 cell MoG3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUTufYJoUW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC55OU[zJO69VQ>? MlTJV2FPT0WU
human SW954 cell NV76d4h{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUnUS4hqUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m1OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDN4M{Wg{txO NF\2N4RUSU6JRWK=
human BE-13 cell MlrDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2rk[2lvcGmkaYTpc44hd2ZiaIXtZY4hSkVvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg1PDF6IN88US=> NV3BcWRCW0GQR1XS
human MOLT-4 cell NV7VUlVkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGjoS5pKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl7N{ig{txO NGD4S|FUSU6JRWK=
human NBsusSR cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1POU2lvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQTF|OEeg{txO M2XBSnNCVkeHUh?=
human H9 cell M1T2XWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1\mbmlvcGmkaYTpc44hd2ZiaIXtZY4hUDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkmyPVc6KM7:TR?= MkT6V2FPT0WU
human A172 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEPqRZNKdmirYnn0bY9vKG:oIHj1cYFvKEFzN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlk5PDFzIN88US=> NWjkN3BDW0GQR1XS
human ES5 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWrJcohq[mm2aX;uJI9nKGi3bXHuJGVUPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMECyOFgh|ryP MV;TRW5ITVJ?
human SBC-1 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2LuSWlvcGmkaYTpc44hd2ZiaIXtZY4hW0KFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlA{QTJ6IN88US=> M372OXNCVkeHUh?=
human NCI-H209 cell MonES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnjqTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTZ4MEKg{txO MU\TRW5ITVJ?
human NKM-1 cell Ml;zS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVHDOGRxUW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTZ5OUig{txO NYLpcmNzW0GQR1XS
human NCI-H720 cell NHm0SoRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Moj1TY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEeyNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOjB4Mkeg{txO MoDBV2FPT0WU
human KE-37 cell MoDGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1[2V2lvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIyOzh6IN88US=> NFvkcIxUSU6JRWK=
human SW48 cell M2ntbmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmTrTY5pcWKrdHnvckBw\iCqdX3hckBUXzR6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yN|E2PSEQvF2= MWnTRW5ITVJ?
human IST-SL1 cell Mn7yS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M17ER2lvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLWPMNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzF5Mkeg{txO MoDhV2FPT0WU
human SK-NEP-1 cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU6HUD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{PjR7ODFOwG0> NUe4XoZWW0GQR1XS
human NOMO-1 cell NWHzZWdmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG5QVU9vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|Y4OjVizszN NG\GTpZUSU6JRWK=
human DOHH-2 cell NXr3[ZV{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M36yVGlvcGmkaYTpc44hd2ZiaIXtZY4hTE:KSD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODJ5NjFOwG0> MoLoV2FPT0WU
human ABC-1 cell Ml3YS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWC4emZuUW6qaXLpeIlwdiCxZjDoeY1idiCDQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDB|NUKg{txO NYTGcVR6W0GQR1XS
human Ramos-2G6-4C10 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoTTTY5pcWKrdHnvckBw\iCqdX3hckBT[W2xcz2yS|YuPENzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOFA3ODVizszN NEDDd|BUSU6JRWK=
human EM-2 cell M1XuTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHT1bGJKdmirYnn0bY9vKG:oIHj1cYFvKEWPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlQyPzJzIN88US=> MV\TRW5ITVJ?
human NB14 cell NELvPZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2HseWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVU3OjFizszN NX7yfo1kW0GQR1XS
human MOLT-13 cell MoGzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYf5UIpDUW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVE{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53NkewOkDPxE1? M4DWdXNCVkeHUh?=
human ECC10 cell NV21XFZUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYTJcohq[mm2aX;uJI9nKGi3bXHuJGVESzFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|M2OyEQvF2= NF3YOW9USU6JRWK=
human LK-2 cell NGD3d|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIm0WHVKdmirYnn0bY9vKG:oIHj1cYFvKEyNLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY1PTl2IN88US=> M1TQfnNCVkeHUh?=
human CTB-1 cell NEPaVWFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1TQSGlvcGmkaYTpc44hd2ZiaIXtZY4hS1SELUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY4ODh{IN88US=> MmDzV2FPT0WU
human NCI-H1581 cell NHjEVHNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3PRVmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVixOVgyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS54N{W1JO69VQ>? M4P5SnNCVkeHUh?=
human COLO-800 cell M1LmWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV3pRnd7UW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVgxOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwN{CzPFIh|ryP MnTOV2FPT0WU
human NB7 cell MmXKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJG5DPyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwN{WyPVch|ryP MlTiV2FPT0WU
human LAMA-84 cell Mn7US5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4XsbGlvcGmkaYTpc44hd2ZiaIXtZY4hVEGPQT24OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPzV3MjFOwG0> MnXQV2FPT0WU
human HCT-116 cells Mke4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3vrcGlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDB7MEig{txO MnnzV2FPT0WU
SK-UT-1 cell MlHTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1fvSWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvVWStNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDF3MzFOwG0> MoD4V2FPT0WU
human H4 cell NIOyWpdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVjHN3hVUW6qaXLpeIlwdiCxZjDoeY1idiCKNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPFE2PzhizszN NUDqOXc4W0GQR1XS
human CAL-51 cell NHfaT4VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2n5bGlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUWxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45Ozh2NTFOwG0> NYfoeZh5W0GQR1XS
human LoVo cells M2WxSnBzd2yrZnXyZZRqd25iYYPzZZk> MkTkO|IhcA>? NYLIXoh7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMc3ZwKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQu[mG|ZXSgW3NVQCC{ZXHn[Y51KGG|c3H5MEBKSzVyPUGuPUDPxE1? MUmyOVI4ODRyMx?=
human HN cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS57MkWxJO69VQ>? NXLhSZJxW0GQR1XS
human L-363 cell M1zyTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXjvWYdEUW6qaXLpeIlwdiCxZjDoeY1idiCOLUO2N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQTVzMjFOwG0> NIK1WlVUSU6JRWK=
human NCI-H747 cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIPPWGtKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IO|Q4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi5yM{O1N{DPxE1? NIHkR4VUSU6JRWK=
human A498 cell NV\rb3ZmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUPJcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkO2Olkh|ryP MXjTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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In vitro kinase assays :

Recombinant Aurora A and B are expressed as NH2-terminal His6-tagged fusion proteins using a baculovirus expression system. Aurora A is purified by affinity chromatography using Ni-NTA agarose, and Aurora B is purified by ion exchange chromatography using CM Sepharose Fast Flow. 1 ng purified recombinant enzyme is added to a reaction cocktail containing 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM peptide substrate, 10 μM for Aurora A or 5 μM ATP for Aurora B, and 0.2 μCi γ-[33P]ATP (specific activity ≥2,500 Ci/mmol), and is then incubated at RT for 60 minutes. Reactions are stopped by addition of 20% phosphoric acid, and the products are captured on P30 nitrocellulose filters and assayed for incorporation of 33P with a BetaplateTM counter. No enzyme and no compound control values are used to determine the concentration of ZM447439, which gave 50% inhibition of enzyme activity. Further details are available on request from Nicholas Keen.
Cell Research:[2]
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  • Cell lines: BON, QGP-1 and MIP-101 cells
  • Concentrations: 0-5 μM
  • Incubation Time: 72 hours
  • Method: Cell number is evaluated by crystal violet staining. In brief, cells in 96-well plates are fixed with 1% glutaraldehyde. Then cells are stained with 0.1% crystal violet. The unbound dye is removed by washing with water. Bound crystal violet is solubilized with 0.2% Triton X-100. Light extinction which increases linearly with the cell number is analyzed at 570 nm using an ELISA reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.59
Formula

C29H31N5O4

CAS No. 331771-20-1
Storage powder
in solvent
Synonyms N/A

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Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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