Alisertib (MLN8237)

Catalog No.S1133

For research use only.

Alisertib (MLN8237) is a selective Aurora A inhibitor with IC50 of 1.2 nM in a cell-free assay. It has >200-fold higher selectivity for Aurora A than Aurora B. Alisertib induces cell cycle arrest, apoptosis and autophagy. Phase 3.

Alisertib (MLN8237) Chemical Structure

CAS No. 1028486-01-2

Selleck's Alisertib (MLN8237) has been cited by 305 publications

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Other Aurora Kinase Products

Biological Activity

Description Alisertib (MLN8237) is a selective Aurora A inhibitor with IC50 of 1.2 nM in a cell-free assay. It has >200-fold higher selectivity for Aurora A than Aurora B. Alisertib induces cell cycle arrest, apoptosis and autophagy. Phase 3.
Features First orally available inhibitor of Aurora A.
Targets
Aurora A [1]
(Cell-free assay)
1.2 nM
In vitro

MLN8237 shows >200-fold higher selectivity for Aurora A than the structurally related Aurora B with an IC50 of 396.5 nM, and does not have any significant activity against 205 other kinases. [1] MLN8237 (0.5 μM) treatment inhibits the phosphorylation of Aurora A in MM1.S and OPM1 cells, without affecting the Aurora B mediated histone H3 phosphorylation. MLN8237 significantly inhibits cell proliferation in multiple myeloma (MM) cell lines with IC50 values of 0.003-1.71 μM. MLN8237 displays more potent anti-proliferation activity against primary MM cells and MM cell lines in the presence of BM stroma cells, as well as IL-6 and IGF-1 than against MM cells alone. MLN8237 (0.5 μM) induces 2- to 6-fold increase in G2/M phase in primary MM cells and cell lines, as well as significant apoptosis and senescence, involving the up-regulation of p53, p21 and p27, as well as PARP, caspase 3, and caspase 9 cleavage. In addition, MLN8237 shows strong synergistic anti-MM effect with dexamethasone, as well as additive effect with doxorubicin and bortezomib. [2] MLN8237 (0.5 μM) treatment causes the inhibition of colony formation of FLO-1, OE19, and OE33 esophageal adenocarinoma cell lines, and induces a significant increase in the percentage of polyploid cells, and subsequently an increase in the percentage of cells in the sub-G1 phase, which can be further enhanced in combination with cisplatin (2.5 μM), involving the higher induction of TAp73β, PUMA, NOXA, cleaved caspase-3, and cleaved PARP as compared with a single-agent treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 Mk\oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjKflUxNjVizszN NVT6Vo9QPzJiaB?= NUPvfplsTE2VTx?= M3myPWlEPTB;MD6wOEDPxE1? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF|Nk[4OEc,OjZzM{[2PFQ9N2F-
LS174T MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIS1cWUxNjVizszN NIX5cWo4OiCq NVLw[YZyTE2VTx?= M4\YbWlEPTB;MD6wOUDPxE1? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF|Nk[4OEc,OjZzM{[2PFQ9N2F-
T84 NEPCW4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrze|IxOC53IN88US=> NF\m[JM4OiCq NG[2TGVFVVOR NF3EfnVKSzVyPUCuNFkh|ryP NW\SRm9VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xN|Y3QDRpPkK2NVM3Pjh2PD;hQi=>
LS180 Mnz0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWf5OG53OC53IN88US=> M3P6b|czKGh? MYPEUXNQ M3\4TWlEPTB;MTFOwG0> NET3R5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGzOlY5PCd-Mk[xN|Y3QDR:L3G+
SW948 M3vIWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;SR|AvPSEQvF2= NH;CcpU4OiCq MULEUXNQ NYnsOGV[UUN3ME2xJO69VQ>? Ml:5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzM{[2PFQoRjJ4MUO2Olg1RC:jPh?=
HCT15 M1nIPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDofIsxNjVizszN MXW3NkBp MWLEUXNQ M3qySGlEPTB:MD60JO69VQ>? M{L4b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUO2Olg1Lz5{NkGzOlY5PDxxYU6=
DLD-1 Mnz0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37RPVAvPSEQvF2= NF7CVos4OiCq M3jkfWROW09? MWjJR|UxRDBwODFOwG0> MnzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzM{[2PFQoRjJ4MUO2Olg1RC:jPh?=
MIP-101 NEfoPVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4q2W|AvPSEQvF2= NXy5WFNjPzJiaB?= Ml\ESG1UVw>? MmPCTWM2OD1zIN88US=> NUXsepVURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xN|Y3QDRpPkK2NVM3Pjh2PD;hQi=>
SNU1544 M{D6U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTZTJMxNjVizszN NIK4[Jc4OiCq MWjEUXNQ MYrJR|UxRTFizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF|Nk[4OEc,OjZzM{[2PFQ9N2F-
OCI-Ly10 MUDDfZRwfG:6aXOgRZN{[Xl? MmPZO|IhcA>? MlvaSG1UVw>? NFP1V4FKSzVyPUCuNFU5KM7:TR?= M{DhUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe4N|MyLz5{NUi3PFM{OTxxYU6=
SU-DHL2 NVHCbohQS3m2b4TvfIlkKEG|c3H5 MXm3NkBp MYDEUXNQ M2nXO2lEPTB;MD6wNUDPxE1? NXrEOZE6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O|g{OzFpPkK1PFc5OzNzPD;hQi=>
OCI-LY7 MY\DfZRwfG:6aXOgRZN{[Xl? MUG3NkBp M4HIbWROW09? NXK1RoU{UUN3ME2wMlA5OSEQvF2= M1vreFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe4N|MyLz5{NUi3PFM{OTxxYU6=
SU-DHL6 NWHpUpNGS3m2b4TvfIlkKEG|c3H5 NFWzRlU4OiCq MnzwSG1UVw>? M2nZXmlEPTB;MD60PFIh|ryP NI\PSFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi3PFM{OSd-MkW4O|g{OzF:L3G+
Jeko-1 NG\meWZEgXSxdH;4bYMhSXO|YYm= M4DaVVczKGh? M1\KTWROW09? MonNTWM2OD1yLkCyPUDPxE1? NIDR[oM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi3PFM{OSd-MkW4O|g{OzF:L3G+
JVM-2 M2jBcWN6fG:2b4jpZ{BCe3OjeR?= NHr4emQ4OiCq MVLEUXNQ M4rjcGlEPTB;MD6wNUDPxE1? M4O2WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe4N|MyLz5{NUi3PFM{OTxxYU6=
Rec-1 MUDDfZRwfG:6aXOgRZN{[Xl? MWS3NkBp MY\EUXNQ MWLJR|UxRTBwMEi3JO69VQ>? NUPyPFJVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O|g{OzFpPkK1PFc5OzNzPD;hQi=>
Z-138 M{fNW2N6fG:2b4jpZ{BCe3OjeR?= NVTiR3FnPzJiaB?= M4XjOWROW09? MnzXTWM2OD1yLkCxN{DPxE1? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh5OEOzNUc,OjV6N{izN|E9N2F-
H9 MnTyR5l1d3SxeHnjJGF{e2G7 NUn2fFUzPzJiaB?= NH:3XlJFVVOR NH3H[4NKSzVyPUCuOkDPxE1? NETBfnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi3PFM{OSd-MkW4O|g{OzF:L3G+
HH NHnHd2VEgXSxdH;4bYMhSXO|YYm= NXzPTVVqPzJiaB?= M4LEVmROW09? MWXJR|UxRTBwNzFOwG0> M{DC[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe4N|MyLz5{NUi3PFM{OTxxYU6=
DND41 MYPDfZRwfG:6aXOgRZN{[Xl? M2nvNFczKGh? NI\yeXdFVVOR MnvITWM2OD1yLkGg{txO MlzqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{izN|EoRjJ3OEe4N|MyRC:jPh?=
CCL119 MXHDfZRwfG:6aXOgRZN{[Xl? M1TLfVczKGh? NF3jWnhFVVOR NXO5U|B1UUN3ME2wMlA3OiEQvF2= Mn2yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{izN|EoRjJ3OEe4N|MyRC:jPh?=
J.Cam 1.6 NGPrd4lEgXSxdH;4bYMhSXO|YYm= Mn3XO|IhcA>? NWrrSYY1TE2VTx?= NYPuV|VsUUN3ME2wMlExPSEQvF2= MmnjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6N{izN|EoRjJ3OEe4N|MyRC:jPh?=
Sup-T1 NG\HS45EgXSxdH;4bYMhSXO|YYm= Mmn3O|IhcA>? M3PX[WROW09? MXXJR|UxRTJwMUSyJO69VQ>? M4DjU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEe4N|MyLz5{NUi3PFM{OTxxYU6=
Tib 152 MnzoR5l1d3SxeHnjJGF{e2G7 MVq3NkBp M3rYXGROW09? NH7rc|hKSzVyPUCuPEDPxE1? NHrpWIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi3PFM{OSd-MkW4O|g{OzF:L3G+
MCF7 M2THU2Z2dmO2aX;uJGF{e2G7 MYW1JO69VQ>? NFrFZYkzPCCq M2jG[WROW09? MXHJcoR2[2W|IFeyM20h[XK{ZYP0 NX;RW|R[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q1ODFpPkK1PFM1PDBzPD;hQi=>
MDA-MB-231 MUfGeY5kfGmxbjDBd5NigQ>? M2HDc|Uh|ryP MV[yOEBp MXzEUXNQ MU\JcoR2[2W|IFezM20h[XK{ZYP0 NYjHO3RnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q1ODFpPkK1PFM1PDBzPD;hQi=>
MCF7 M3;6NWZ2dmO2aX;uJGF{e2G7 NFfObHU2KM7:TR?= NVG0NnBCOjRiaB?= MVTEUXNQ MnHMSIVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVtKG:oIFPET|EwS0SFMh?= NFz1bVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUizOFQxOSd-MkW4N|Q1ODF:L3G+
MCF7 MYnGeY5kfGmxbjDBd5NigQ>? NYnZ[W1mPSEQvF2= MnPzNlQhcA>? Mm\ISG1UVw>? M3;EemRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDs[ZZmdCCxZjDDSGsz MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNUc,OjV6M{S0NFE9N2F-
MCF7 MU\GeY5kfGmxbjDBd5NigQ>? M2GzZ|Uh|ryP Mny3NlQhcA>? M3K3bmROW09? NGjN[4NF\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gcIV3\Wxib3[gZ5lkdGmwIFKx NFfINFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUizOFQxOSd-MkW4N|Q1ODF:L3G+
MCF7 NGnpeYxHfW6ldHnvckBCe3OjeR?= NW\K[YVFPSEQvF2= NYHofWd{OjRiaB?= MY\EUXNQ MWPJcoNz\WG|ZYOgeIhmKGW6cILld5Nqd25ibHX2[Ywhd2ZicEKxJHdi\jFxQ3nwNS=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNUc,OjV6M{S0NFE9N2F-
MCF7 NXy4UGVqTnWwY4Tpc44hSXO|YYm= NEj0NmU2KM7:TR?= NX2xNpFMOjRiaB?= M173dGROW09? M1PMRWlv[3KnYYPld{B1cGViZYjwdoV{e2mxbjDs[ZZmdCCxZjDwNlchU2myMR?= NELoSJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUizOFQxOSd-MkW4N|Q1ODF:L3G+
MDA-MB-231 M4n4W2Z2dmO2aX;uJGF{e2G7 NYH1NHY3PSEQvF2= M4Lub|I1KGh? NYTRW|JmTE2VTx?= M4PuNWRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDs[ZZmdCCxZjDDSGsyN0OGQ{K= M2Hp[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAyLz5{NUizOFQxOTxxYU6=
MDA-MB-231 NFrvfmRHfW6ldHnvckBCe3OjeR?= NHrrS44yKM7:TR?= NHzkN2wzPCCq NWLiN41qTE2VTx?= Ml\LTY5kemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVtKG:oIFPET|I> M{T2Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAyLz5{NUizOFQxOTxxYU6=
MDA-MB-231 MoXqSpVv[3Srb36gRZN{[Xl? M{jLXVUh|ryP MVGyOEBp NF7MUoJFVVOR MWrE[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ibHX2[Ywhd2ZiY4njcIlvKEJz MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNUc,OjV6M{S0NFE9N2F-
MDA-MB-231 NHXUUZVHfW6ldHnvckBCe3OjeR?= MXK1JO69VQ>? NU\1bXZGOjRiaB?= NHzzNGFFVVOR MoDTTY5kemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVtKG:oIICyNUBY[WZzL1PpdFE> NIDnOXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUizOFQxOSd-MkW4N|Q1ODF:L3G+
MDA-MB-231 M1uwbmZ2dmO2aX;uJGF{e2G7 NH3Jcm42KM7:TR?= NUPjSHJPOjRiaB?= MVfEUXNQ NVS4c4ROUW6lcnXhd4V{KHSqZTDlfJBz\XO|aX;uJIxmfmWuIH;mJJAzPyCNaYCx MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNUc,OjV6M{S0NFE9N2F-
MDA-MB-231 NGXYXndHfW6ldHnvckBCe3OjeR?= M{LnOlUh|ryP NV;FV5psOjRiaB?= NX70cpVQTE2VTx?= NFztRXVKdmO{ZXHz[ZMhfGinIHX4dJJme3Orb36gcIV3\Wxib3[gdFU{ M2K0VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAyLz5{NUizOFQxOTxxYU6=
MCF7 NYX2S4xRSXCxcITvd4l{KEG|c3H5 MVy1JO69VQ>? Mo\QNlQhcA>? MmDESG1UVw>? M{LlPGlv\HWlZYOgZZBweHSxdHnjJIRm[XSq MnX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6M{S0NFEoRjJ3OEO0OFAyRC:jPh?=
MDA-MB-231 M3jHTGFxd3C2b4Ppd{BCe3OjeR?= NVu3[VRiPSEQvF2= Mkm1NlQhcA>? Mn;JSG1UVw>? NHrCZoFKdmS3Y3XzJIFxd3C2b4TpZ{Bl\WG2aB?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh|NESwNUc,OjV6M{S0NFE9N2F-
MCF7 MnPJSpVv[3Srb36gRZN{[Xl? MVOxJO69VQ>? MWO3NkBp MWrEUXNQ MXXJcoR2[2W|IHH1eI9xcGGpaXOg[IVifGh? NXPV[pk{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q1ODFpPkK1PFM1PDBzPD;hQi=>
MDA-MB-231 NGfkOVhHfW6ldHnvckBCe3OjeR?= M3H2dVEh|ryP NFvRcpY4OiCq MYjEUXNQ M1\kdGlv\HWlZYOgZZV1d3CqYXfpZ{Bl\WG2aB?= M33i[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0OFAyLz5{NUizOFQxOTxxYU6=
U-2 OS MnSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWn6OYxpPTBizszN M{LsSFI1KGh? NWXmcXJFTE2VTx?= Mnz5TWM2OD1zNj62JO69VQ>? MlewQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OUK4NVEoRjJ3N{myPFEyRC:jPh?=
MG-63 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFP6dXA2OCEQvF2= NH;FdY8zPCCq NHTOR2hFVVOR M3Gx[2lEPTB;OT61JO69VQ>? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd7MkixNUc,OjV5OUK4NVE9N2F-
U-2 OS M2W0UGFxd3C2b4Ppd{BCe3OjeR?= M4DiXFUh|ryP MV2yOEBp MnriSG1UVw>? NH3DU4NKdmS3Y3XzJIFxd3C2b4TpZ{Bk\WyuIHTlZZRp NV;xc|RyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PVI5OTFpPkK1O|kzQDFzPD;hQi=>
MG-63 M1XGfWFxd3C2b4Ppd{BCe3OjeR?= NU[5cZRVPSEQvF2= Mkn5NlQhcA>? MYnEUXNQ MXLJcoR2[2W|IHHwc5B1d3SrYzDj[YxtKGSnYYTo M3GwTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{myPFEyLz5{NUe5NlgyOTxxYU6=
U-2 OS NVKxNJlbTnWwY4Tpc44hSXO|YYm= M3zoZ|Uh|ryP M3fwOVI1KGh? NICxV3dFVVOR MlHxVJJwdW:2ZYOgZZV1d3CqYXfpZ{Bk\WyuIHTlZZRp NVewbZpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PVI5OTFpPkK1O|kzQDFzPD;hQi=>
MG-63 NWOyNZdCTnWwY4Tpc44hSXO|YYm= MVO1JO69VQ>? MmHvNlQhcA>? M3vaWGROW09? MkXBVJJwdW:2ZYOgZZV1d3CqYXfpZ{Bk\WyuIHTlZZRp MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd7MkixNUc,OjV5OUK4NVE9N2F-
PANC-1 NUTub21OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkD2OVAh|ryP MX6yOEBp MWHEUXNQ MUDJR|UxRTdwMTFOwG0> NVfBRodLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|IzOjVpPkK1OlMzOjJ3PD;hQi=>
BxPC-3 NETSd4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXu1NEDPxE1? MV:yOEBp MkKySG1UVw>? NFuzOnZKSzVyPU[uPEDPxE1? M4DuT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkOyNlI2Lz5{NU[zNlIzPTxxYU6=
PANC-1 NFPTbohHfW6ldHnvckBCe3OjeR?= NULTZXF{PSEQvF2= NIPzWWYzPCCq M1S5ZWROW09? MVnJcoR2[2W|IHPlcIwh[3mlbHWgZZJz\XO2IHnuJGczN01icHjhd4U> NYG5eYRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|IzOjVpPkK1OlMzOjJ3PD;hQi=>
BxPC-3 NIPCRotHfW6ldHnvckBCe3OjeR?= MlG3OUDPxE1? NFHqbokzPCCq NXLqd3ZNTE2VTx?= M2S4cGlv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgbY4hTzJxTTDwbIF{\Q>? NHzsU5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[zNlIzPSd-MkW2N|IzOjV:L3G+
PANC-1 MWfGeY5kfGmxbjDBd5NigQ>? MXO1JO69VQ>? NV3GPIkzOjRiaB?= M2nOSWROW09? M4j3R2lv\HWlZYOgZZV1d3CqYXfpZ{Bk\WyuIHTlZZRp MnTvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{KyNlUoRjJ3NkOyNlI2RC:jPh?=
BxPC-3 NHjHdpdHfW6ldHnvckBCe3OjeR?= M1q5elUh|ryP NV7lVI9wOjRiaB?= NVKwUoRkTE2VTx?= MmfjTY5lfWOnczDheZRweGijZ3njJINmdGxiZHXheIg> NXzMPYg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2N|IzOjVpPkK1OlMzOjJ3PD;hQi=>
SKOV3 M365XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPaWJJNOTByIN88US=> MUCyOEBp NYDDR5FxTE2VTx?= NVjvdoFvUUN3ME2yNE41QCEQvF2= NVXXUYQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NlQ4PTBpPkK1OlI1PzVyPD;hQi=>
OVCAR4 M1Hib2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXk[W0yODBizszN MYOyOEBp NVTQXmFDTE2VTx?= NHjpVYhKSzVyPUKyMlE{KM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ{NEe1NEc,OjV4MkS3OVA9N2F-
SKOV3 MWnGeY5kfGmxbjDBd5NigQ>? NWfjR5hLPSEQvF2= NVP0bGs1PzJiaB?= M3rJZWROW09? M2X4PWlv\HWlZYOgS|IwVSCjcoLld5Q> NH\BcpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[yOFc2OCd-MkW2NlQ4PTB:L3G+
OVCAR4 M1LnT2Z2dmO2aX;uJGF{e2G7 NEW4dmo2KM7:TR?= NFrRZnM4OiCq MV7EUXNQ MUDJcoR2[2W|IFeyM20h[XK{ZYP0 NVS1PGM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NlQ4PTBpPkK1OlI1PzVyPD;hQi=>
SKOV3 MnTGRZBweHSxc3nzJGF{e2G7 MY[1JO69VQ>? M{HJR|I1KGh? Ml\1SG1UVw>? MUjJcoR2[2W|IHHwc5B1d3Orcx?= MmTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MkS3OVAoRjJ3NkK0O|UxRC:jPh?=
OVCAR4 NWXGbpp5SXCxcITvd4l{KEG|c3H5 NF;6NFA2KM7:TR?= NXjN[XdkOjRiaB?= NYLrRnhVTE2VTx?= NH21UZBKdmS3Y3XzJIFxd3C2b4Ppdy=> MnXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MkS3OVAoRjJ3NkK0O|UxRC:jPh?=
AGS M2KzWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYWyOUDPxE1? NUnkRm1ZOjRiaB?= MmjWSG1UVw>? NWjFbIRtUUN3ME2xPU4xQSEQvF2= NF7lTWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[wPVkzOyd-MkW2NFk6OjN:L3G+
NCI-N78 NELQfnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTzVmRqOjVizszN NF;3eFEzPCCq MonZSG1UVw>? MYDJR|UxRTJ4LkOzJO69VQ>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZyOUmyN{c,OjV4MEm5NlM9N2F-
AGS NEHhbZpCeG:ydH;zbZMhSXO|YYm= NIrRZZk2KM7:TR?= MYWyOEBp MoXESG1UVw>? NVLYW2xRUW6mdXPld{BieG:ydH;zbZM> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZyOUmyN{c,OjV4MEm5NlM9N2F-
NCI-N78 NGnvNXZCeG:ydH;zbZMhSXO|YYm= MXS1JO69VQ>? MkOzNlQhcA>? MULEUXNQ NIr0O|RKdmS3Y3XzJIFxd3C2b4Ppdy=> M{j2c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkC5PVI{Lz5{NU[wPVkzOzxxYU6=
AGS MUfGeY5kfGmxbjDBd5NigQ>? NVP0bG9iPSEQvF2= MYWyOEBp NXjHdW97TE2VTx?= M33oOmlv\HWlZYOgeIhmKGG3dH;wbIFogQ>? NFHBXYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[wPVkzOyd-MkW2NFk6OjN:L3G+
NCI-N78 MX3GeY5kfGmxbjDBd5NigQ>? MV21JO69VQ>? MY[yOEBp NX7pOI5HTE2VTx?= Mlv4TY5lfWOnczD0bIUh[XW2b4DoZYd6 M3myNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkC5PVI{Lz5{NU[wPVkzOzxxYU6=
HSC-3 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUOxJO69VQ>? NF7Ge2M1QCCq MoT6TWM2OD1yLkW0JO69VQ>? NEH2XlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO2OlE1Oyd-MkWzOlYyPDN:L3G+
GB30 NYHZOXFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnZNUDPxE1? NIP2SlM4KGR? NIDYZXhFVVOR M3XGXmlEPTB;MD6wNVEh|ryP NUD0codSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNFY1OjhpPkK1NVA3PDJ6PD;hQi=>
GB9 NIPt[JVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfodIIyKM7:TR?= NEHoUHI4KGR? MVLEUXNQ NH7KUnpKSzVyPUCuNFI1KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNkSyPEc,OjVzME[0Nlg9N2F-
GB169 NXvnRml4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7SNUDPxE1? MVy3JIQ> M3zsRmROW09? NF\MbpVKSzVyPUCuNFMzKM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFyNkSyPEc,OjVzME[0Nlg9N2F-
T24 NVK0S4pnTnWwY4Tpc44hSXO|YYm= M2TMTFEh|ryP MXG0PEBp M3fjb2ROW09? NH:1N2xKdmS3Y3XzJINmdGxiY4njcIUh[XK{ZYP0 NIHjdHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwN|Y{Oyd-MkO0NFM3OzN:L3G+
RT4 M3GzeWZ2dmO2aX;uJGF{e2G7 Mn;NNUDPxE1? NUXBXmdJPDhiaB?= MXzEUXNQ MYrJcoR2[2W|IHPlcIwh[3mlbHWgZZJz\XO2 NHHNcXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwN|Y{Oyd-MkO0NFM3OzN:L3G+
UM-UC-3 NWjZ[GJITnWwY4Tpc44hSXO|YYm= MYqxJO69VQ>? NUXSSZB[PDhiaB?= MorZSG1UVw>? NG\zdnFKdmS3Y3XzJINmdGxiY4njcIUh[XK{ZYP0 M1uzPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NECzOlM{Lz5{M{SwN|Y{OzxxYU6=
T24 MmroRZBweHSxc3nzJGF{e2G7 MWmzMlE3KM7:TR?= MoXwPVYhcA>? NWXaXm1STE2VTx?= NYfUflZrUUN3ME2wMlA{ODZizszN M1TTU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NECzOlM{Lz5{M{SwN|Y{OzxxYU6=
RT4 NGHyWIlCeG:ydH;zbZMhSXO|YYm= M3u1eFMvOTZizszN NULNNoRXQTZiaB?= M3PoV2ROW09? NEHaVXBKSzVyPUCuNVE6QCEQvF2= Mly3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEO2N|MoRjJ|NECzOlM{RC:jPh?=
UM-UC-3 MWjBdI9xfG:|aYOgRZN{[Xl? NVjaboZJOy5zNjFOwG0> NG\rXXU6PiCq MWHEUXNQ NXz6VGxxUUN3ME2wMlA1PDlizszN M37PPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NECzOlM{Lz5{M{SwN|Y{OzxxYU6=
OVCAR-5 MlHTSpVv[3Srb36gRZN{[Xl? MmSyOVAhdk1? MVzJcohq[mm2czDj[YxtKG2rZ4LheIlwdg>? MlntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|M{SzNlcoRjJ|M{O0N|I4RC:jPh?=
SKOV3ip2 M3fpbmZ2dmO2aX;uJGF{e2G7 NVW4fXBCPTBibl2= M{LsPWlvcGmkaYTzJINmdGxibXnndoF1cW:w MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN|NEOyO{c,OjN|M{SzNlc9N2F-
S462 MnXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHG[ohWOTByIN88US=> Mnz5O|IhcA>? MULEUXNQ NI\4fXpCfHSnboXheIV{KGOnbHyg[5Jwf3Sq MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{OEGxOEc,OjN|MkixNVQ9N2F-
2884 NXm5RpdrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTObWk6OTByIN88US=> MWm3NkBp MX\EUXNQ MmnqRZR1\W63YYTld{Bk\WyuIHfyc5d1cA>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{OEGxOEc,OjN|MkixNVQ9N2F-
2885 M3Pofmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrPVWFmOTByIN88US=> M3HhblczKGh? NWTCWo9HTE2VTx?= NVrRPHQzSXS2ZX71ZZRmeyClZXzsJIdzd3e2aB?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{OEGxOEc,OjN|MkixNVQ9N2F-
CRL-2396 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3:yeVExOCEQvF2= NHjqRVZ4[XSnch?= NFHCeW9KSzVyPUCuNFkzKM7:TR?= NIHYT|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G1N|UzPCd-MkOxOVM2OjR:L3G+
TIB-48 NHvkS|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;JNVAxKM7:TR?= NWfmc5E{f2G2ZYK= M1\lUGlEPTB;MD6wPFgh|ryP NHm5boI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G1N|UzPCd-MkOxOVM2OjR:L3G+
CRL-2396 NFPtdHVEgXSxdH;4bYMhSXO|YYm= MoLGNUDPxE1? M3zMe|Q5KGh? NUPNT|Rqf2G2ZYK= MWHJcoR2[2W|IHHwc5B1d3Orcx?= M{LGPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUWzOVI1Lz5{M{G1N|UzPDxxYU6=
TIB-48 MYLDfZRwfG:6aXOgRZN{[Xl? M2PacVEh|ryP M3jrUVQ5KGh? M17NfZdifGW{ Ml7tTY5lfWOnczDhdI9xfG:|aYO= M2CxWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUWzOVI1Lz5{M{G1N|UzPDxxYU6=
AGS NUXjdY1VS3m2b4TvfIlkKEG|c3H5 NW\Md4NFOC53IN88US=> MVWyOEBp MWfEUXNQ M1HJUGRm[3KnYYPld{Bk\WyuIIP1dpZqfmGu M3zDVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUeyOlEyLz5{Mkm3NlYyOTxxYU6=
FLO-1 MkHQR5l1d3SxeHnjJGF{e2G7 NXvqZYdlOC53IN88US=> M4rt[FI1KGh? MYjEUXNQ MXjE[YNz\WG|ZYOgZ4VtdCC|dYL2bZZidA>? NF7kbHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm3NlYyOSd-MkK5O|I3OTF:L3G+
OE33 MYnDfZRwfG:6aXOgRZN{[Xl? NWjGW4h2OC53IN88US=> Mo\UNlQhcA>? NVLi[Xc{TE2VTx?= MlO2SIVkemWjc3XzJINmdGxic4Xyeol3[Wx? MlTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7N{K2NVEoRjJ{OUeyOlEyRC:jPh?=
SKLMS NHnvZnhEgXSxdH;4bYMhSXO|YYm= Mkj6O|Uhdk1? M4jKeVk3KGh? M3e0Rmlv\HWlZYOgZZBweHSxc3nz NUHh[5hNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlE6QTdpPkKyPFIyQTl5PD;hQi=>
Leio285 MUDDfZRwfG:6aXOgRZN{[Xl? M{TIXFc2KG6P Mn3FPVYhcA>? NFTkS2dKdmS3Y3XzJIFxd3C2b4Ppdy=> NYLOclc1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlE6QTdpPkKyPFIyQTl5PD;hQi=>
Mes-Sa M3vXVGN6fG:2b4jpZ{BCe3OjeR?= NY\GdVV{PzVibl2= MVW5OkBp M1LGemlv\HWlZYOgZZBweHSxc3nz NIfifHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyNVk6Pyd-MkK4NlE6QTd:L3G+
DAOY NG\ZV25EgXSxdH;4bYMhSXO|YYm= MojwNVAh|ryP MoLPO|IhcA>? NWrMSHltTE2VTx?= NV\2ZnRmUUN3ME2wMlA1KM7:TR?= NH;4S5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[2PVM{PSd-MkK2Olk{OzV:L3G+
IMR32 M32wR2N6fG:2b4jpZ{BCe3OjeR?= NH3EPWYyOCEQvF2= NIi1NW04OiCq M2DldGROW09? M2LWemlEPTB;MD6wN{DPxE1? MojOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NkmzN|UoRjJ{Nk[5N|M2RC:jPh?=
Molt-4 MlrXR5l1d3SxeHnjJGF{e2G7 MWSxNEDPxE1? NWLGelFsPzJiaB?= M1GwV2ROW09? MYHJR|UxRTBwMEKg{txO NGnKWJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mk[2PVM{PSd-MkK2Olk{OzV:L3G+
MOLM-13 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPZfGlMOyEQvF2= M3zjWlczKGh? MXTEbY1qdmm|aHXzJINmdGxidnnhZoltcXS7 NHezUYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkS4PFI1QSd-MkK0PFgzPDl:L3G+
HL-60 NYHGPWk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrDUWc{KM7:TR?= MW[3NkBp NV\qTnFKTGmvaX7pd4hmeyClZXzsJJZq[WKrbHn0fS=> NULhbHo{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFgzPDlpPkKyOFg5OjR7PD;hQi=>
MV4-11 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvsN{DPxE1? NFvKVmk4OiCq NF\O[XlFcW2rbnnzbIV{KGOnbHygeoli[mmuaYT5 NXLJ[GhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFgzPDlpPkKyOFg5OjR7PD;hQi=>
SKM-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn23N{DPxE1? MoexO|IhcA>? NXrQRVhmTGmvaX7pd4hmeyClZXzsJJZq[WKrbHn0fS=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6OEK0PUc,OjJ2OEiyOFk9N2F-
SH2 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LteFMh|ryP NWjON|FCPzJiaB?= M2HaeGRqdWmwaYPo[ZMh[2WubDD2bYFjcWyrdIm= M2XT[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEi4NlQ6Lz5{MkS4PFI1QTxxYU6=
NOMO-1 NIKxdIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjKNVRQOyEQvF2= NULjWnRXPzJiaB?= NITHbmlFcW2rbnnzbIV{KGOnbHygeoli[mmuaYT5 MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6OEK0PUc,OjJ2OEiyOFk9N2F-
OCL-AML2 M1zsR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHON{DPxE1? MofFO|IhcA>? NI\1bIZFcW2rbnnzbIV{KGOnbHygeoli[mmuaYT5 MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR6OEK0PUc,OjJ2OEiyOFk9N2F-
PL-21 NHj3WnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XqbVMh|ryP NILFRWU4OiCq MYPEbY1qdmm|aHXzJINmdGxidnnhZoltcXS7 NXHvdIN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFgzPDlpPkKyOFg5OjR7PD;hQi=>
KG-1 NVj1b3dQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrVfZU{KM7:TR?= MXK3NkBp MXjEbY1qdmm|aHXzJINmdGxidnnhZoltcXS7 M17mVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEi4NlQ6Lz5{MkS4PFI1QTxxYU6=
A172 NFPX[m1EgXSxdH;4bYMhSXO|YYm= MXGxNFAh|ryP NF2yR2MzPCCq MWnEUXNQ M3rqOmlEPTB;MD6xNlAh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ5NEO5PUc,OjJ{N{SzPVk9N2F-
U87 MYHDfZRwfG:6aXOgRZN{[Xl? NIjBbogyODBizszN Ml3KNlQhcA>? NVy0WnRUTE2VTx?= MkLWTWM2OD1yLkGwOUDPxE1? M2C0OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mke0N|k6Lz5{MkK3OFM6QTxxYU6=
U251 M2Tj[WN6fG:2b4jpZ{BCe3OjeR?= MomxNVAxKM7:TR?= NWnQVZNjOjRiaB?= NET5[ZBFVVOR MYXJR|UxRTBwMUCwJO69VQ>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ5NEO5PUc,OjJ{N{SzPVk9N2F-
T98 MlXlR5l1d3SxeHnjJGF{e2G7 Mny3NVAxKM7:TR?= NFLYPHkzPCCq NF\VXZZFVVOR NEHwUGdKSzVyPUCuNVI2KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ5NEO5PUc,OjJ{N{SzPVk9N2F-
LN18 M4XEWWN6fG:2b4jpZ{BCe3OjeR?= M2f4flExOCEQvF2= NFf1c4kzPCCq NEfrOWFFVVOR M4rPOWlEPTB;MD6yNVAh|ryP NVHycWd5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO|Q{QTlpPkKyNlc1Ozl7PD;hQi=>
LN443 NVLRbo1bS3m2b4TvfIlkKEG|c3H5 MnnsNVAxKM7:TR?= MkTpNlQhcA>? NH:yRpVFVVOR NHX2RplKSzVyPUCuNlIxKM7:TR?= NVPEb4VmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO|Q{QTlpPkKyNlc1Ozl7PD;hQi=>
HF66 MmDZR5l1d3SxeHnjJGF{e2G7 M3jUVFExOCEQvF2= Mo\LNlQhcA>? MXPEUXNQ NXvlVVM{UUN3ME2wMlIzPSEQvF2= NFXUN4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK3OFM6QSd-MkKyO|Q{QTl:L3G+
HF2303 MkHJR5l1d3SxeHnjJGF{e2G7 MXexNFAh|ryP NH3URVczPCCq M4jDRWROW09? NVzKcmhEUUN3ME2wMlA3OCEQvF2= NV\OcXFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyO|Q{QTlpPkKyNlc1Ozl7PD;hQi=>
HF2359 NXHneJpjS3m2b4TvfIlkKEG|c3H5 MVWxNFAh|ryP NHPaNHQzPCCq NHXCZVFFVVOR NHnDbYJKSzVyPUCuNFYxKM7:TR?= M4iwRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mke0N|k6Lz5{MkK3OFM6QTxxYU6=
HF2414 MkPoR5l1d3SxeHnjJGF{e2G7 Mn3ONVAxKM7:TR?= NE\BfnozPCCq MYfEUXNQ MX3JR|UxRTBwMEiwJO69VQ>? NH3xTWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK3OFM6QSd-MkKyO|Q{QTl:L3G+
A-673 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17kXFExKM7:TR?= M4PPV|k3KGh? M4DufGROW09? Ml[4TWM2OD1yLkCzNkDPxE1? MojsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NEi1PVEoRjJzNES4OVkyRC:jPh?=
TC-32 NF:0SVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV:xNEDPxE1? NFHpT|k6PiCq NW[2W|YyTE2VTx?= M{nYS2lEPTB;MD6wN|kh|ryP M1H4cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
TC-71 NW\BNZo{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPINVAh|ryP NIXtS5M6PiCq MWTEUXNQ MVXJR|UxRTBwMUCyJO69VQ>? MkGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NEi1PVEoRjJzNES4OVkyRC:jPh?=
SK-N-MC NEj1[mZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrRcpQyOCEQvF2= M37PUVk3KGh? NEfFToZFVVOR MVnJR|UxRTBwMEeyJO69VQ>? Mlv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NEi1PVEoRjJzNES4OVkyRC:jPh?=
CHLA-9 Ml;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGGzSXEyOCEQvF2= MnLOPVYhcA>? Mlu3SG1UVw>? M3G2N2lEPTB;MD6wNVgh|ryP M{jj[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
CHLA-10 NE\IcpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFK3fpYyOCEQvF2= NHTKOGM6PiCq NWrzeGN5TE2VTx?= M3[2U2lEPTB;MD6wOlAh|ryP NUG0O5lxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
CHLA-25 NYHXdZI4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHpNVAh|ryP NEXuRYU6PiCq NFXyR|VFVVOR MX\JR|UxRTBwMU[4JO69VQ>? MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NEi1PVEoRjJzNES4OVkyRC:jPh?=
CHLA-32 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33ZfFExKM7:TR?= NUnlTIlTQTZiaB?= MnLqSG1UVw>? MXjJR|UxRTBwMUO2JO69VQ>? M4nw[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
CHLA-56 NIfORWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{iyOFExKM7:TR?= Mo\IPVYhcA>? Mn3HSG1UVw>? NFnkRoVKSzVyPUGwJO69VQ>? NVj1[2hCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
CHLA-258 MonIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYqxNEDPxE1? M4C4fVk3KGh? NGC5d2pFVVOR M3LRdmlEPTB;MD6xN|Ih|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR2OEW5NUc,OjF2NEi1PVE9N2F-
COG-E-352 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWmxNEDPxE1? NX62WFVSQTZiaB?= NXHPb5N[TE2VTx?= M3L2VWlEPTB;MD6wOFMh|ryP NUTSe2VFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
CHLA-90 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV2xNEDPxE1? MmXSPVYhcA>? MkXQSG1UVw>? MnHYTWM2OD1yLkC2NUDPxE1? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR2OEW5NUc,OjF2NEi1PVE9N2F-
CHLA-119 M4\CNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXj2NJIxOTBizszN NXPDZXRiQTZiaB?= NH;0dIRFVVOR NYH2VHVbUUN3ME2wMlAzOiEQvF2= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR2OEW5NUc,OjF2NEi1PVE9N2F-
CHLA-122 MmjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHD[4tiOTBizszN MUS5OkBp M1vKUWROW09? MUnJR|UxRTBwMEG5JO69VQ>? NHnsfZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS0PFU6OSd-MkG0OFg2QTF:L3G+
CHLA-136 NVT5SJQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\kNVAh|ryP M{jUUlk3KGh? MoTtSG1UVw>? NF;V[XJKSzVyPUCuNFM6KM7:TR?= M1fzWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
CHLA-140 M2DMWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYexNEDPxE1? M3W1XFk3KGh? NIjkbI5FVVOR MlzHTWM2OD1yLkCyOkDPxE1? M334T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
LA-N-6 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYO5WppWOTBizszN MXK5OkBp NVnHeIdGTE2VTx?= NXHRc4NRUUN3ME2wMlA2PCEQvF2= M4LYWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
NB-1643 MkfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn2zNVAh|ryP Mmn3PVYhcA>? NH;YbmRFVVOR NYD4fZRmUUN3ME2wMlA{PyEQvF2= MnXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NEi1PVEoRjJzNES4OVkyRC:jPh?=
NB-EBc1 MoT6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLHSFZPOTBizszN Mkn1PVYhcA>? NXvIUIVnTE2VTx?= NWrBUY9lUUN3ME2wMlA2OCEQvF2= NWjUfFNORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
SK-N-BE-1 M3XzfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULpdYQyOTBizszN MWW5OkBp M{DCU2ROW09? M{jP[2lEPTB;MD6wNlgh|ryP NFftNZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS0PFU6OSd-MkG0OFg2QTF:L3G+
SK-N-BE-2 NYfLRlRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnPNVAh|ryP NWfhUllzQTZiaB?= MnfSSG1UVw>? MoTlTWM2OD1yLkCzOkDPxE1? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR2OEW5NUc,OjF2NEi1PVE9N2F-
SMS-KAN MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXLTm94OTBizszN M1vrcVk3KGh? MVrEUXNQ MmPJTWM2OD1yLkCzOEDPxE1? NUjpWJJjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
SMS-KANR NVPzc2d6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljjNVAh|ryP NVvXdIZFQTZiaB?= MULEUXNQ NInVVGRKSzVyPUCuNFI3KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR2OEW5NUc,OjF2NEi1PVE9N2F-
SMS-KCN MofTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXqxNEDPxE1? NVPKUmtkQTZiaB?= NXf1cHFGTE2VTx?= MmezTWM2OD1yLkCxPUDPxE1? M1\JRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
SMS-KCNR MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7iVGZ2OTBizszN MUS5OkBp MX\EUXNQ M{fSemlEPTB;MD6wNVAh|ryP NVfS[YpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
SMS-LHN MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTINVAh|ryP M17NSVk3KGh? M{fDNmROW09? M2nsWGlEPTB;MD6wN|Ih|ryP NY\nUYhvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG0OFg2QTFpPkKxOFQ5PTlzPD;hQi=>
SMS-MSN MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHw[msyOCEQvF2= MkS0PVYhcA>? NXL4TGNRTE2VTx?= M2\rc2lEPTB;MD6wNlIh|ryP M4ezWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNES4OVkyLz5{MUS0PFU6OTxxYU6=
SMS-SAN NIDaRZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXWxNEDPxE1? M1zOflk3KGh? MoC3SG1UVw>? Mlm4TWM2OD1yLkCyNEDPxE1? NEOy[4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUS0PFU6OSd-MkG0OFg2QTF:L3G+
Granta-4 Mn34R5l1d3SxeHnjJGF{e2G7 MVuxNEDPxE1? NEO3SoQ4KGR? Mn33TWM2OD1yLkC0NEDPxE1? M4jITlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxPFY4Lz5{MUK5NVg3PzxxYU6=
DB NHLFdG1EgXSxdH;4bYMhSXO|YYm= M1;2N|ExKM7:TR?= MYK3JIQ> MlLkTWM2OD1yLkC0NkDPxE1? NXPRVXNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVE5PjdpPkKxNlkyQDZ5PD;hQi=>
RL NUW2Nm5yS3m2b4TvfIlkKEG|c3H5 NVG5[WdmOTBizszN M3W3cVch\A>? NVfBb25GUUN3ME2wMlAyPSEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUi2O{c,OjF{OUG4Olc9N2F-
K562 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjkZWFpOTBizszN MXu5OkBp NF3YNG5KSzVyPUCuNFg4KM7:TR?= MkDKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOUG2N|MoRjJzMEmxOlM{RC:jPh?=
LAMA-84 Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUmxNEDPxE1? MnW5PVYhcA>? MYrJR|UxRTBwMEW3JO69VQ>? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB7MU[zN{c,OjFyOUG2N|M9N2F-
MM15 MoPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDLOEDPxE1? M1O3[|czKGh? Ml;xSG1UVw>? MX\JR|UxRTBwMUOg{txO MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN6Mki0OEc,OjB|OEK4OFQ9N2F-
OPM1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVG0JO69VQ>? NGTDfHg4OiCq NFjaXJpFVVOR NUjj[HU4UUN3ME2wMlA{KM7:TR?= M3rJTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{iyPFQ1Lz5{MEO4Nlg1PDxxYU6=
RPM1 MlfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUG0JO69VQ>? NFP6d5Y4OiCq MXjEUXNQ NX62SoRLUUN3ME2xNE4{OiEQvF2= M3fiVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{iyPFQ1Lz5{MEO4Nlg1PDxxYU6=
INA6 Mn6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{L6XVQh|ryP NULifGl2PzJiaB?= MnXsSG1UVw>? MVzJR|UxRTBwMECyJO69VQ>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN6Mki0OEc,OjB|OEK4OFQ9N2F-
OPM2 NXnzU|A3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHjOEDPxE1? NUjXUYFOPzJiaB?= MY\EUXNQ NHPzXFVKSzVyPUSuN|ch|ryP M3nnN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{iyPFQ1Lz5{MEO4Nlg1PDxxYU6=
MM1R NXfUd29iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX60JO69VQ>? MV63NkBp MnSwSG1UVw>? MX3JR|UxRTFwNkig{txO MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN6Mki0OEc,OjB|OEK4OFQ9N2F-
DOX40 NE\2NWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXe0JO69VQ>? NYDadVJTPzJiaB?= M1LnU2ROW09? MlnxTWM2OD13LkS4JO69VQ>? M4PlWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{iyPFQ1Lz5{MEO4Nlg1PDxxYU6=
LR5 M2TI[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTLOEDPxE1? Ml\1O|IhcA>? NVixfZNxTE2VTx?= MWPJR|UxRTJwNUOg{txO MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN6Mki0OEc,OjB|OEK4OFQ9N2F-
U266 MnHuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWq0R2JJPCEQvF2= NXLFbpprPzJiaB?= NUDIcJgzTE2VTx?= MWLJR|UxRTFwNEOg{txO Ml;IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|OEK4OFQoRjJyM{iyPFQ1RC:jPh?=
RD M4Hmc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjm[|AyOCEQvF2= MoPVPVYhcA>? M4jTRWlEPTB;MD6yNlgh|ryP M1\3VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUC4N|M5Lz5{MEGwPFM{QDxxYU6=
Rh41 M{XzTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHizOo8yOCEQvF2= NUS2Sld1QTZiaB?= M1e0RWlEPTB;MD6wPVAh|ryP MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODFyOEOzPEc,OjBzMEizN|g9N2F-
Rh30 MlnlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmn2NVAh|ryP NFfONJA6PiCq MYXJR|UxRTBwMkOwJO69VQ>? NILyU4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGwPFM{QCd-MkCxNFg{Ozh:L3G+
BT-12 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXTWGsyOCEQvF2= NWTHNXV3QTZiaB?= M2T0Z2lEPTB;MD6wOlAh|ryP NIiyRpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGwPFM{QCd-MkCxNFg{Ozh:L3G+
CHLA-266 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWiyTY1HOTBizszN NHvscY06PiCq NWW2UnloUUN3ME2wMlA4OiEQvF2= NFvjeJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGwPFM{QCd-MkCxNFg{Ozh:L3G+
TC-71 NIXYTHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HSO|ExKM7:TR?= NFjkR5c6PiCq M1G3ZWlEPTB;MD6xNFIh|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODFyOEOzPEc,OjBzMEizN|g9N2F-
SJ-GBM2 M4nsNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zZbFExKM7:TR?= NGnEbWk6PiCq M4PqXmlEPTB;MD6wOVAh|ryP M1r5WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUC4N|M5Lz5{MEGwPFM{QDxxYU6=
NALM-6 NHXQ[IVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfQUVdLOTBizszN M3PsOVk3KGh? MUfJR|UxRTBwME[yJO69VQ>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODFyOEOzPEc,OjBzMEizN|g9N2F-
COG-LL-317 NF7TVW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvYNVAh|ryP NIXGdpA6PiCq M1jzfGlEPTB;MD6wOFch|ryP M3vFclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUC4N|M5Lz5{MEGwPFM{QDxxYU6=
RS4-11 MnrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ntWFExKM7:TR?= Mn7lPVYhcA>? MUnJR|UxRTBwMEG4JO69VQ>? NV3IZ3dERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxNFg{OzhpPkKwNVA5OzN6PD;hQi=>
MOLT-4 MmLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrtNVAh|ryP MkDCPVYhcA>? MoTJTWM2OD1yLkCyOkDPxE1? MkPKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzMEizN|goRjJyMUC4N|M5RC:jPh?=
CCRF-CEM MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3yzc|ExKM7:TR?= NITtNXU6PiCq NEC1XYtKSzVyPUCuNFk1KM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODFyOEOzPEc,OjBzMEizN|g9N2F-
Kasumi-1 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLxNVAh|ryP MWq5OkBp Moj2TWM2OD1yLkGwN{DPxE1? NHrMUYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGwPFM{QCd-MkCxNFg{Ozh:L3G+
Karpas-299 NE\1fFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{WyNlExKM7:TR?= MlLFPVYhcA>? NFe0cHNKSzVyPUCuNFM5KM7:TR?= NFywW2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEGwPFM{QCd-MkCxNFg{Ozh:L3G+
Ramos-RA1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLFRWQxOTBizszN NWrH[ZJ5QTZiaB?= NFHrR3ZKSzVyPUCuNVI4KM7:TR?= M3;oOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUC4N|M5Lz5{MEGwPFM{QDxxYU6=
GSS M3LxcGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWX5VFZPPzJiaILz MmjFRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCJU2OgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFeVVEigZZN{[XluIFnDOVAhRSByLkCzPUDPxE1w M2f5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkK1OlE4Lz5{NU[yOVYyPzxxYU6=
LU99A NF\OUHZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{DudVczKGi{cx?= MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyXOUnBJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCZU2S4JIF{e2G7LDDJR|UxKD1iMD6wOlIh|ryPLh?= M1fMRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkK1OlE4Lz5{NU[yOVYyPzxxYU6=
HL60 NWDK[otCSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NG[xS|g4OiCqcoO= NYDaWGFWSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3Q5KGG|c3H5MEBKSzVyIE2gNE4xPzRizszNMi=> Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MkW2NVcoRjJ3NkK1OlE4RC:jPh?=
LC2/ad MmH1RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NFfJNGk4OiCqcoO= M3jGfWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTFOyM4FlKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3Q5KGG|c3H5MEBKSzVyIE2gNE4xPzdizszNMi=> NX\zbWh6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NlU3OTdpPkK1OlI2PjF5PD;hQi=>
MKN45 NEP4UllCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4q3UFczKGi{cx?= M{XjeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVvOOFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGfTWFgh[XO|YYmsJGlEPTBiPTCwMlA6OyEQvF2u NHm3bIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[yOVYyPyd-MkW2NlU3OTd:L3G+
HCT116 MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Ml7YO|IhcHK| MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB5MjDodpMh[nliV2PUPEBie3OjeTygTWM2OCB;IECuNFk2KM7:TT6= NHf4d5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[yOVYyPyd-MkW2NlU3OTd:L3G+
Lu116 M1fTcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M1\JcFczKGi{cx?= NXj0O|J4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMeVEyPiClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVQCCjc4PhfUwhUUN3MDC9JFAvODl5IN88UU4> M4DlRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkK1OlE4Lz5{NU[yOVYyPzxxYU6=
NCI-H358 NX7Tb3NSSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEfTO5c4OiCqcoO= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST3IN|U5KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3Q5KGG|c3H5MEBKSzVyIE2gNE4yKM7:TT6= NGP0bG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[yOVYyPyd-MkW2NlU3OTd:L3G+
MIAPaCa2 NEXzSFFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4[zV|czKGi{cx?= M4[yOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVnBVIFE[TJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHdUXDhiYYPzZZktKEmFNUCgQUAxNjF|IN88UU4> MoXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MkW2NVcoRjJ3NkK1OlE4RC:jPh?=
PC14 NXPnd3pbSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MWC3NkBpenN? M2DM[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUFOxOEBk\WyuczDh[pRmeiB5MjDodpMh[nliV2PUPEBie3OjeTygTWM2OCB;IECuNVch|ryPLh?= NYHnbFJCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NlU3OTdpPkK1OlI2PjF5PD;hQi=>
HT-29 MYPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MYW3NkBpenN? NXfiNpZYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVQCCjc4PhfUwhUUN3MDC9JFAvOzNizszNMi=> NH\LRVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[yOVYyPyd-MkW2NlU3OTd:L3G+
HCT15 MkTJRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MkS2O|IhcHK| MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEG1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCZU2S4JIF{e2G7LDDJR|UxKD1iMD63OEDPxE1w Mmi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MkW2NVcoRjJ3NkK1OlE4RC:jPh?=
Sf9 MVrGeY5kfGmxbjDhd5NigQ>? MoT1R49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBud3W|ZTDheZJwemFia3nuZZNmKEFiZYjwdoV{e2WmIHnuJIlve2WldDDT[lkh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiQWTQMEBMcSB;IECuNFAxOyEQvF2u M4ezN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUCxOVY1Lz5{NkGwNVU3PDxxYU6=
Sf9 MoDFSpVv[3Srb36gZZN{[Xl? NWPHd2JsUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBud3W|ZTDheZJwemFia3nuZZNmKEFiZYjwdoV{e2WmIHnuJIlve2WldDDT[lkh[2WubIOgeZNqdmdiYnnveIlvNUeOUmLBV2xIKGG|IIP1ZpN1emG2ZTDpckBxemW|ZX7j[UBw\iCdZ3HtcYEuOzOSXVHUVEwhUUN3MDC9JFAvODBzIN88UU4> M3HqSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUCxOVY1Lz5{NkGwNVU3PDxxYU6=
HCT116 MUnGeY5kfGmxbjDhd5NigQ>? MniyTY5pcWKrdHnvckBw\iCjdYLvdoEhc2mwYYPlJGEh[XW2b4Doc5NxcG:{eXzheIlwdiCjdDDUNlg5KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDifUBqdW23bn;mcJVwemW|Y3XuZ4Uh[W6jbInzbZMtKEmFNUCgQUAxNjByNzFOwG0v MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFyMUW2OEc,OjZzMEG1OlQ9N2F-
HCT116 NX;xeGJoS3m2b4TvfIlkcXS7IHHzd4F6 MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[nliQoLkWUBqdmOxcoDvdoF1cW:wIHHzd4F6NCCJSUWwJF0hOC5yMzFOwG0v M4HXblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUCxOVY1Lz5{NkGwNVU3PDxxYU6=
HCT116 M2PUVGZ2dmO2aX;uJIF{e2G7 MnPHTY5pcWKrdHnvckBw\iCjdYLvdoEhc2mwYYPlJGIhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiaHnzeI9v\SCKMzDwbI9{eGixconsZZRqd25iYomgbY1ufW6xZnz1c5Jme2OnbnPlJIFv[Wy7c3nzMEBKSzVyIE2gNU42KM7:TT6= MnXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzMEG1OlQoRjJ4MUCxOVY1RC:jPh?=
BL21 (DE3) Rosetta MnfUSpVv[3Srb36gZZN{[Xl? MVyzNEBucW6| NWHPZ|BLUW6qaXLpeIlwdiCxZjDIbZMufGGpZ3XkJIh2dWGwIFH1do9z[SCDIHvpcoF{\SBqMUKyJJRwKDRyIILld4llfWW|KTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIDjESVMqKFKxc3X0eIEh[2WubIOgeZNqdmdiYnnveIlvgWyjdHXkJHNVUzJic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhOzBibXnud{BjgSCKVGLGJIF{e2G7LDDJR|UxKD1iMD6wNFAxPCEQvF2u NEnTPXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5NVE{Oyd-MkezPVEyOzN:L3G+
HeLa Kyoto NYP3V3loTnWwY4Tpc44h[XO|YYm= NXzvV|NjOjBiaILz NWH5b445UW6qaXLpeIlwdiCxZjDBeZJwemFiQjDrbY5ie2ViaX6gbJVu[W5iSHXMZUBMgW:2bzDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36g[Il{fHKrYoX0bY9vKG:oIIDod5Bpdy2qaYP0c45mKEh|IIPldlExKGyndnXsJIlv[3WkYYTl[EBnd3JiMkCgbJJ{NCCLQ{WwJF0hOC5yMEG1JO69VS5? NH\T[XI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5NVE{Oyd-MkezPVEyOzN:L3G+
HeLa Kyoto MVzGeY5kfGmxbjDhd5NigQ>? NWPXV|duOjBiaILz M3\CVGlvcGmkaYTpc44hd2ZiQYXyc5JiKEFia3nuZZNmKGG3dH;wbI9{eGixconsZZRqd25iYYSgWIhzOjh6IHnuJIh2dWGwIFjlUIEhU3mxdH:gZ4VtdHNiaX7jeYJifGWmIH\vdkAzOCCqcoOsJGlEPTBiPTCwMlAxPjdizszNMi=> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzN7MUGzN{c,Ojd|OUGxN|M9N2F-
multiple myeloma MofWSpVv[3Srb36gZZN{[Xl? M1G3UHN2eHC{ZYPzbY9vKG:oIHPlcIwhdWm2b4Ppd{BqdiCqdX3hckBufWy2aYDs[UBugWWub33hJINmdGy|LDDJR|UxKD1iMD6wNFMh|ryPLh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDlzOEC5Okc,Ojh7MUiwPVY9N2F-
Calu6 NHznZ4hCdnSrdIXtc5Ih[XO|YYm= M2\mbFIxKG2pL3vn NXTtS3dWOjFiZHH5dy=> MUjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFYXz1OkBk\WyuczD4[Y5w\3KjZoTl[EBqdiCvb4Xz[UBie3Onc4Pl[EBieyC2dX3vdkBoem:5dHigbY5pcWKrdHnvckBifCB{MDDt[{9s\yxicH:gZollKGGmbXnubZN1\XKnZDDmc5IhOjFiZHH5dy=> NIX5c|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkGwNVU3PCd-Mk[xNFE2PjR:L3G+
HeLa Kyoto MXPGeY5kfGmxbjDhd5NigQ>? MXSwMlI2KHWP MWmyNEBpenN? NVPkTHVvUW6qaXLpeIlwdiCxZjDBeZJwemFiQTDrbY5ie2VibH;jZYxqgmG2aX;uJIF1KHOyaX7kcIUhdWmlcn;0eYJ2dGW|IHnuJIh2dWGwIFjlUIEhU3mxdH:gZ4VtdHNiYYSgNE4zPSC3TTDpcoN2[mG2ZXSg[o9zKDJyIHjydy=> NGe0c4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O5NVE{Oyd-MkezPVEyOzN:L3G+
MDA-MB-231 NFLISHdHfW6ldHnvckBie3OjeR?= MmnlNUB2VQ>? MWq0PEBpenN? NXLh[IxNUW6mdXP0bY9vKG:oIHPodo9ud3OxbXWgZYxq\26vZX70JIRm\mWldIOgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyKHWPIHHmeIVzKDR6IHjyd{BjgSCGQWDJJJN1[WmwaX7nJIJie2WmIHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>? M2K5e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{W4NVQ4Lz5{OUO1PFE1PzxxYU6=
MDA-MB-231 Mmi0SpVv[3Srb36gZZN{[Xl? NF65O|EyKHWP NGrqcFk1QCCqcoO= MVrJcoR2[3Srb36gc4Yh[WKncoLhcpQhe3CrbnTs[UBnd3KvYYTpc44hf2m2aDD0dolxd2yjcjDhcoQhfGW2cnHwc4xieiCxY3P1dpJmdmOnIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNUB2VSCjZoTldkA1QCCqcoOgZpkhTEGSSTDzeIFqdmmwZzDiZZNm\CCrbX31co9ndHWxcnXzZ4Vv[2ViYYPzZZk> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTN3OEG0O{c,Ojl|NUixOFc9N2F-
MDA-MB-231 MYnGeY5kfGmxbjDhd5NigQ>? M{DDW|AvPSC3TR?= NUjoeFdIPDhiaILz NELjWnRKdmirYnn0bY9vKG:oIHHsdIhiNXS3YoXsbY4hcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyCjYn;sbZNpdWWwdDDv[kBz\We3bHHyJIxw[2G2aX;uJI9nKHC{b4TlbY4h[XRiMD61JJVOKGGodHXyJFQ5KGi{czDifUBFSVCLIIP0ZYlvcW6pIHLhd4VlKGmvbYXuc4ZtfW:{ZYPj[Y5k\SCjc4PhfS=> MmK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl|NUixOFcoRjJ7M{W4NVQ4RC:jPh?=
MDA-MB-231 NIPLfXVHfW6ldHnvckBie3OjeR?= NYrvVpVqOC53IIXN NV3GNoxVPDhiaILz MojYTY5pcWKrdHnvckBw\iCDVWLLRUBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGGkb3zpd4hu\W62IH;mJJJm\3WuYYKgcI9k[XSrb36gc4YheHKxdHXpckBifCByLkWgeW0h[W[2ZYKgOFghcHK|IHL5JGRCWElic4ThbY5qdmdiYnHz[YQhcW2vdX7v[ox2d3Knc3PlcoNmKGG|c3H5 M1;tNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7M{W4NVQ4Lz5{OUO1PFE1PzxxYU6=
TC32 MmjudWhVWyCjc4PhfS=> M1z4VJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| MlS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 MXTxTHRUKGG|c3H5 Mm\5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= MnPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH NFjQVHdyUFSVIHHzd4F6 NHqxN25yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> NG\pcFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MVHxTHRUKGG|c3H5 M2jVXZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= M2\URVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MkTFdWhVWyCjc4PhfS=> MmD4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= M3fNXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 M1XXcpFJXFNiYYPzZZk> M2roUJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DNUWEY{GgZ4VtdHN? NIX1dIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NGi1ZphyUFSVIHHzd4F6 NXj5O5ZueUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
U-2 OS NG\wcmpyUFSVIHHzd4F6 MUfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDVMVIhV1NiY3XscJM> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 MoHhdWhVWyCjc4PhfS=> M1zkdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz NX34[nZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC M{HGV5FJXFNiYYPzZZk> MVzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 NWmyWXMxeUiWUzDhd5NigQ>? MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= NWHmNWdVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 MYLxTHRUKGG|c3H5 MoK2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NIPXe5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 MorFdWhVWyCjc4PhfS=> Mn\ndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? MoDlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC M3f5[JFJXFNiYYPzZZk> MnfmdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>? M4\PZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32 NFTBOHZyUFSVIHHzd4F6 M33rSJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NWjud|hpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NInZU2VyUFSVIHHzd4F6 MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Assay
Methods Test Index PMID
Western blot p-AURKA(T288) / p-EIF4E(S209) / c-Myc ; phospho-Aurora A / Aurora B ; H3S10P / H3K27me2 / H3K27me3 / H3K9me2 / H3AcK / H4K16Ac 28073841 22863010 29477140
Growth inhibition assay Cell viability 25632225
Immunofluorescence acetylated α-tubulin / γ-tubulin ; E-cadherin / β-catenin / vimentin / p-SMAD5 ; Centrin-2 / tubulin ; phospho-Aurora A(T288) 29401581 23334326 30899434 20382844
In vivo MLN8237 significantly reduces the tumor burden with tumor growth inhibition (TGI) of 42% and 80% at 15 mg/kg and 30 mg/kg, respectively, and prolongs the survival of mice compared with the control. [2]

Protocol (from reference)

Kinase Assay:[1]
  • Aurora A radioactive Flashplate enzyme assay:

    Aurora A radioactive Flashplate enzyme assay is conducted to determine the nature and degree of MLN8237-mediated inhibition in vitro. Recombinant Aurora A is expressed in Sf9 cells and purified with GST affinity chromatography. The peptide substrate for Aurora A is conjugated with biotin (Biotin-GLRRASLG). Aurora A kinase (5 nM) is assayed in 50 mM Hepes (pH 7.5), 10 mM MgCl2, 5 mM DTT, 0.05% Tween 20, 2 μM peptide substrate, 3.3 μCi/mL [γ-33P]ATP at 2 μM, and increasing concentrations of MLN8237 by using Image FlashPlates.

Cell Research:[2]
  • Cell lines: MM1.S, MM.1R, LR5, RPMI 8226, DOX40, OPM1, OPM2, INA6, and U266
  • Concentrations: Dissolved in DMSO, final concentrations ~10 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of MLN8237 for 24, 48, and 72 hours. Cells viability is measured using MTT assay, and cell proliferation is measured using 3[H]-thymidine incorporation. For cell cycle analysis, cells are permeabilized by 70% ethanol at -20 °C, and incubated with 50 μg/mL PI and 20 units/mL RNase-A. DNA content is analyzed by flow cytometry using BDFACS-Canto II and FlowJo software. For the detection of apoptosis and senescence, cells are stained with fluorescein isothiocyanate-annexin V and PI. Apoptotic cells are determined by flow cytometric analysis using BDFACS-Canto II and FlowJo software.
Animal Research:[2]
  • Animal Models: Severe combined immune-deficient (SCID) mice inoculated subcutaneously with MM1.S cells
  • Dosages: ~30 mg/kg/day
  • Administration: Orally

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+30% PEG300+5%Tween-80+ddH2O
For best results, use promptly after mixing.

6mg/mL

Chemical Information

Molecular Weight 518.92
Formula

C27H20ClFN4O4

CAS No. 1028486-01-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C(=CC=C1)F)C2=NCC3=CN=C(N=C3C4=C2C=C(C=C4)Cl)NC5=CC(=C(C=C5)C(=O)O)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04479306 Active not recruiting Drug: Alisertib|Drug: Osimertinib|Drug: Sapanisertib Recurrent Lung Non-Small Cell Carcinoma|Stage IIIB Lung Cancer AJCC v8|Stage IV Lung Cancer AJCC v8|Stage IVA Lung Cancer AJCC v8|Stage IVB Lung Cancer AJCC v8 M.D. Anderson Cancer Center June 18 2020 Phase 1
NCT02812056 Withdrawn Drug: Alisertib|Drug: TAK-228 Malignant Neoplasms of Digestive Organs|Malignant Neoplasms of Female Genital Organs|Malignant Neoplasms of Lip Oral Cavity and Pharynx|Malignant Neoplasms of Male Genital Organs M.D. Anderson Cancer Center|Millennium Pharmaceuticals Inc. September 2016 Phase 1
NCT02719691 Completed Drug: Alisertib|Drug: MLN0128 Metastatic Breast Cancer|Solid Tumors University of Colorado Denver May 13 2016 Phase 1
NCT02367352 Terminated Drug: Alisertib|Drug: Paclitaxel Advanced Solid Tumors|Ovarian Cancer|Small Cell Lung Cancer Millennium Pharmaceuticals Inc.|Takeda March 19 2015 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.

Frequently Asked Questions

Question 1:
What is the suggested formulation of this compound for mouse injection(i.p.)?

Answer:
It can be dissolved in 6% DMSO/50% PEG 300/5% Tween 80/ddH2O at 10 mg/ml as a clear solution.

Tags: buy Alisertib (MLN8237) | Alisertib (MLN8237) supplier | purchase Alisertib (MLN8237) | Alisertib (MLN8237) cost | Alisertib (MLN8237) manufacturer | order Alisertib (MLN8237) | Alisertib (MLN8237) distributor