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Pretomanid (PA-824) Bacterial inhibitor

Name: Pretomanid (PA-824)
Brand: Selleck Chemicals
SKU: S1162
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S1162

Pretomanid (PA-824) is an anti-tuberculosis drug for tuberculosis with MIC less than 2.8 μM, currently in Phase 2.

Chemical Structure

Molecular Weight: 359.26

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S116201 DMSO]72 mg/mL]false]Ethanol]16 mg/mL]false]Water]Insoluble]false Purity: 99.99%

99.99

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Chemical Information, Storage & Stability

Molecular Weight	359.26	Formula	C₁₄H₁₂F₃N₃O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	187235-37-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1C(COC2=NC(=CN21)[N+](=O)[O-])OCC3=CC=C(C=C3)OC(F)(F)F

Solubility

In vitro
Batch:

DMSO : 72 mg/mL (200.41 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	0.5% methylcellulose Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (27.83mM)	Taking the 1 mL working solution as an example, take 10 mg of this product, add it to 1 ml of 0.5% methylcellulose clear solution, and mix evenly to form a uniform suspension. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Features	An experimental anti-tuberculosis drug.
Targets/IC₅₀/Ki	tuberculosis ^[1]
In vitro	In vitro, PA-824 exhibits high activity against multidrug-resistant clinical isolates from Asia (India and South Korea) and from throughout the United States (MIC < 1 μg/ml) and is equally active against the drug-sensitive and multidrug-resistant isolates of M. tuberculosis (MICs range, 0.039 to 0.531 μg/ml). ^[1] A recent study shows that single-nucleotide polymorphisms of its resistance genes (fgd1 [Rv0407] and ddn [Rv3547]) don't significantly affect the MICs of this compound (≤ 0.25 μg/ml). ^[2]
In vivo	In the rapid tuberculosis mouse model, PA-824 shows significant anti-microbial activity in a dose-dependent manner: at 50 mg/kg, it in MC produces a more than 1-log reduction of the CFU in the lungs; at 100 mg/kg it produces about a 2-log reduction, and at 300 mg/kg it produces a 3-log reduction. Furthermore, long-term treatment of this compound at 100 mg/kg in cyclodextrin/lecithin also leads to the reduction of the bacterial load below 500 CFU in the lungs and spleen. ^[1] It exhibits time-dependent anti-microbial activity in a murine model of tuberculosis with a maximal observed bactericidal effect of 0.1 log CFU/day over 24 days. ^[3]
References	[1] https://pubmed.ncbi.nlm.nih.gov/15917524/ [2] https://pubmed.ncbi.nlm.nih.gov/21930879/ [3] https://pubmed.ncbi.nlm.nih.gov/20937781/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT02333799	Completed	Pulmonary Tuberculosis	Global Alliance for TB Drug Development	March 2015	Phase 3
NCT01674218	Completed	Tuberculosis	National Institute of Allergy and Infectious Diseases (NIAID)	September 2012	Phase 1
NCT01768273	Completed	Tuberculosis	Global Alliance for TB Drug Development	December 2009	Phase 1
NCT03202693	Completed	Tuberculosis	Global Alliance for TB Drug Development	March 2006	Phase 1

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