Name: Berberine
Brand: Selleck Chemicals
SKU: S9046
Rating: 5 (11 reviews)

Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.

Berberine Chemical Structure

CAS: 2086-83-1

Selleck's Berberine has been cited by 11 publications

Purity & Quality Control

Batch: Purity: 99.70%

99.70

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Choose Selective Bacterial Inhibitors

Biological Activity

Description	Berberine (Umbellatine), an alkaloid isolated from Rhizoma coptidis, has broad applications, particularly as an antibacterial agent in the clinic.

In vitro
In vitro	In 3T3-L1 adipocytes, the treatment of 12 μM or 20 μM berberine combined with 25 μM resveratrol shows more significant inhibition of lipid accumulation compared with individual compounds. The combination of berberine with resveratrol significantly increases the low-density-lipoprotein receptor expression in HepG2 cells to a level about one-fold higher in comparison to individual compounds.^[1]
Cell Research	Cell lines	3T3-L1 adipocytes, HepG2 cells, hepatic L02 cells
	Concentrations	12 μM, 20 μM, 50 μM
	Incubation Time	3 days, 24 h
	Method	At day 10 of differentiation, 3T3-L1 adipocytes are treated with Berberine, resveratrol, and their combination for 3 days. The intracellular lipid accumulation of differentiated cells is detected by oil red O staining. HepG2 cells are cultured in 6-well plate for 24 h with a 3 × 10₅ cell density, and then the culture medium is replaced by fresh medium containing Berberine, resveratrol, and their combination for another 24 h. Subsequently, the total proteins from the cells are extracted for assaying the low-density-lipoprotein receptor (LDLR) expression by Western Blot.

In Vivo
In vivo	The combination of Berberine and resveratrol exerts a significant enhanced effect on lipid-lowering in hyperlipidemic mice and intracellular lipid accumulation-decreasing in adipocytes as compared to individual agent alone, which is closely associated with SIRT1 and LDLR.^[1]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05845931	Recruiting	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	May 5 2023	Not Applicable
NCT05480670	Completed	Polycystic Ovary Syndrome	Ayub Teaching Hospital	November 1 2022	Not Applicable
NCT05463003	Completed	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	July 19 2022	Not Applicable

References

[1] Zhu X, et al. Int J Mol Sci. 2018 Dec 6;19(12):3903.

Chemical Information & Solubility

Molecular Weight	336.36	Formula	C₂₀H₁₈NO₄
CAS No.	2086-83-1	SDF	--
Smiles	COC1=C(C2=C[N+]3=C(C=C2C=C1)C4=CC5=C(C=C4CC3)OCO5)OC
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 22.5 mg/mL ( (66.89 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : 1 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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