ML141
Catalog No.S7686 Synonyms: CID-2950007
Molecular Weight(MW): 407.49
ML141 (CID-2950007), is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7).
3 Customer Reviews
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(A) The survival rate detected at 72 h after the injection of different concentrations of ML141; shrimp injected with DMSO were used as controls. (B) The expression of WSSV IE1 at the mRNA level at 24 h post ML141 injection. Shrimp injected with the same amount of DMSO in each group were used as controls.
J Virol, 2016, doi: 10.1128/JVI.01916-16. . ML141 purchased from Selleck.
Western blot analysis of expressions of E-cadherin and vimentin in HCT116 cells treated with ML141 compared with DMSO-treated cells
J Proteome Res, 2018, 17(1):265-275. ML141 purchased from Selleck.
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Compared with the DMSO group, there were low levels of (D) MMP-9 and (E) MMP-2 expression in the ML141-treated group. There were no statistically significant differences in MMP-9 and MMP-2 expression between ML14-treated and ML141+TAOCSB-treated groups. *P<0.05 or **P<0.01 vs. DMSO group. TAOCSB, total alkaloids of Corydalis saxicola bunting; MMP, matrix metalloproteinase; Cdc42, cell division cycle 42; DMSO, dimethylsulfoxide.
ONCOLOGY LETTERS, 2018, 15: 475-482. ML141 purchased from Selleck.
Purity & Quality Control
Choose Selective Rho Inhibitors
Biological Activity
| Description | ML141 (CID-2950007), is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7). | ||||||
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| In vitro |
ML141 enhances the ability of TMX to suppress BLBC cell growth through both induction of cell death and suppression of cell division. [2] ML141 also significantly protects neuroblastoma cells from metformin-induced apoptosis. [3] Moreover, ML141 diminishes K. pneumoniae invasion in a dose-dependent manner. [4] |
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| Assay |
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| In vivo | In NOD/SCID mice bearing MDA-MB 231 derived tumors, ML141 (1 mg/day i.p.), via inhibition of Cdc42, enables TMX to suppress growth of MDA-MB 231 derived tumors. [2] In addition, ML141 (10 mg/kg i.p.) enhances G-CSF-induced hematopoietic stem and progenitor cell mobilization in mice. [5] |
Protocol
| Kinase Assay: |
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Equilibrium binding assay : Wild-type GST-Cdc42 (4 μM) is bound to GSH-beads overnight at 4°C. Cdc42 on GSH-beads is depleted of nucleotide by incubating with 10 mM EDTA containing buffer for 20 min at 30°C, washing twice with NP- HPS buffer, then re-suspended in the same buffer containing 1 mM EDTA/or 1 mM MgCl2, 1 mM DTT and 0.1% BSA. Cdc42 unbound sites are blocked by incubation of protein–bead complex for 15 min at RT. Thirty μL of this suspension is incubated with 20 mM inhibitor for 3 min at RT and added 30 μL of various concentrations of ice cold BODIPY-FL-GTP. Samples incubated at 4° C for 45 min and binding of fluorescent nucleotide to enzyme is measured using an Accuri flow cytometer. Raw data are exported and plotted using GraphPad Prism software. |
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Solubility (25°C)
| In vitro | DMSO | 81 mg/mL warmed (198.77 mM) |
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| Water | Insoluble | |
| Ethanol | Insoluble | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. |
10mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 407.49 |
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| Formula | C22H21N3O3S |
| CAS No. | 71203-35-5 |
| Storage | powder |
| in solvent | |
| Synonyms | CID-2950007 |
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