ML141
Catalog No.S7686 Synonyms: CID-2950007
Molecular Weight(MW): 407.49
ML141 (CID-2950007), is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7).
Cited by 3 Publications
3 Customer Reviews
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(A) The survival rate detected at 72 h after the injection of different concentrations of ML141; shrimp injected with DMSO were used as controls. (B) The expression of WSSV IE1 at the mRNA level at 24 h post ML141 injection. Shrimp injected with the same amount of DMSO in each group were used as controls.
J Virol, 2016, doi: 10.1128/JVI.01916-16. . ML141 purchased from Selleck.
Western blot analysis of expressions of E-cadherin and vimentin in HCT116 cells treated with ML141 compared with DMSO-treated cells
J Proteome Res, 2018, 17(1):265-275. ML141 purchased from Selleck.
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Compared with the DMSO group, there were low levels of (D) MMP-9 and (E) MMP-2 expression in the ML141-treated group. There were no statistically significant differences in MMP-9 and MMP-2 expression between ML14-treated and ML141+TAOCSB-treated groups. *P<0.05 or **P<0.01 vs. DMSO group. TAOCSB, total alkaloids of Corydalis saxicola bunting; MMP, matrix metalloproteinase; Cdc42, cell division cycle 42; DMSO, dimethylsulfoxide.
ONCOLOGY LETTERS, 2018, 15: 475-482. ML141 purchased from Selleck.
Purity & Quality Control
Choose Selective Rho Inhibitors
Biological Activity
| Description | ML141 (CID-2950007), is demonstrated to be a potent, selective and reversible non-competitive inhibitor of Cdc42 GTPase suitable for in vitro assays, with IC50 of 200 nM and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7). | ||||||
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| In vitro |
ML141 enhances the ability of TMX to suppress BLBC cell growth through both induction of cell death and suppression of cell division. [2] ML141 also significantly protects neuroblastoma cells from metformin-induced apoptosis. [3] Moreover, ML141 diminishes K. pneumoniae invasion in a dose-dependent manner. [4] |
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| Assay |
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| In vivo | In NOD/SCID mice bearing MDA-MB 231 derived tumors, ML141 (1 mg/day i.p.), via inhibition of Cdc42, enables TMX to suppress growth of MDA-MB 231 derived tumors. [2] In addition, ML141 (10 mg/kg i.p.) enhances G-CSF-induced hematopoietic stem and progenitor cell mobilization in mice. [5] |
Protocol
| Kinase Assay: |
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Equilibrium binding assay : Wild-type GST-Cdc42 (4 μM) is bound to GSH-beads overnight at 4°C. Cdc42 on GSH-beads is depleted of nucleotide by incubating with 10 mM EDTA containing buffer for 20 min at 30°C, washing twice with NP- HPS buffer, then re-suspended in the same buffer containing 1 mM EDTA/or 1 mM MgCl2, 1 mM DTT and 0.1% BSA. Cdc42 unbound sites are blocked by incubation of protein–bead complex for 15 min at RT. Thirty μL of this suspension is incubated with 20 mM inhibitor for 3 min at RT and added 30 μL of various concentrations of ice cold BODIPY-FL-GTP. Samples incubated at 4° C for 45 min and binding of fluorescent nucleotide to enzyme is measured using an Accuri flow cytometer. Raw data are exported and plotted using GraphPad Prism software. |
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Solubility (25°C)
| In vitro | DMSO | 81 mg/mL warmed (198.77 mM) |
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| Water | Insoluble | |
| Ethanol | Insoluble | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing. |
10mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 407.49 |
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| Formula | C22H21N3O3S |
| CAS No. | 71203-35-5 |
| Storage | powder |
| in solvent | |
| Synonyms | CID-2950007 |
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