For research use only.

Catalog No.S7319

13 publications

EHop-016  Chemical Structure

Molecular Weight(MW): 430.55

EHop-016 is a specific Rac GTPase inhibitor with IC50 of 1.1 μM for Rac1 in MDA-MB-435 and MDA-MB-231 cells, equally potent inhibition for Rac3.

Size Price Stock Quantity  
USD 97 In stock
USD 170 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's EHop-016 has been cited by 13 publications

3 Customer Reviews

  • (f) Liver samples from both groups treated with olive oil, CCl4, CCl4+NSC23766 or +EHop-016 for 4 weeks were collected for Rac1 activity assay and western blot analysis.

    Cell Death Dis, 2015, 6:e1751.. EHop-016 purchased from Selleck.

  • Human HCT116 cells were treated with conditioned media of (LPS + Chol)-treated THP-Ms or IL-1b (10 ng/ml) in the absence or presence of Ehop-016 (10 μM), and effects of the indicated factors on b-catenin transcriptional activity of HCT116 cells were determined by the TOP and FOP flash reporter assay, and the protein levels of GTP-Rac1, total Rac1, p-GSK3b (S9), total GSK3b, PCNA and Cyclin D1 were analyzed by western blot (H).

    Biochem Pharmacol, 2016, 105:42-54.. EHop-016 purchased from Selleck.

  • Rac inhibition at day 0 is more effective at reducing differentiation: At confluence, HC11 cells were starved from EGF for 24 h, then induced to differentiate with HIP addition for the indicated number of days. Cells were treated with 10 μM EHop-016 Rac inhibitor for 24 h at day 0 (lanes 8-10), or day 4 (lanes 4-7) or not (lanes 1-3), then induced to differentiate for the indicated number of days through HIP treatment. Detergent cell extracts were probed for β-casein or β-actin as a loading control.

    Exp Cell Res, 2017, 361(1):112-125. EHop-016 purchased from Selleck.

Purity & Quality Control

Choose Selective Rho Inhibitors

Biological Activity

Description EHop-016 is a specific Rac GTPase inhibitor with IC50 of 1.1 μM for Rac1 in MDA-MB-435 and MDA-MB-231 cells, equally potent inhibition for Rac3.
Rac1 [1]
(MDA-MB-435, MDA-MB-231 cells)
1.1 μM
In vitro

Rac inhibition by EHop-016 increases the activity of the closely related Rho GTPase RhoA by a compensatory mechanism. In MDA-MB-435 cells, EHop-016 (2-5 μM) inhibits association of active Vav2 with Rac1(G15A) mutant fusion protein, and reduces Rac-regulated cell functions including lamellipodia formation and cell migration. In addition, EHop-016 inhibits cell viability of MDA-MB-435 cells with IC50 of 10 μM. [1] EHop-016 also inhibits KITD814V-induced growth in SM and AML patients-derived cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-435 cells NVy1e2YxTnWwY4Tpc44h[XO|YYm= MV6yOEBp MXnJcohq[mm2aX;uJI9nKFKjY{GgbY4hcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDh[pRmeiB{NDDodpMh[nliRz3MTXNCKGG|c3H5MEBKSzVyPUGuNUDPxE1? NVnXUYR[OjR3MkC5PVg>

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-AKT / AKT / p-JNK / JNK ; 

PubMed: 25389450     

MDA-MB-435 cells treated with 0, 4, or 8 μM EHop-016 were lysed, and equal amounts of protein subjected to Western blotting followed by integrated density of positive bands. Representative Western blot stained for phospho (p)-Akt, Akt, p-JNK, or JNK.


PubMed: 31314174     

RAC1 activity in KYSE150 and KYSE510 cells treated with 0.0, 2.5, 5.0, and 10.0 μM RAC1 inhibitor Ehop-016 was measured by pull-down assay for RAC1-GTP. Representative western blot images demonstrate positive bands for RAC1‐GTP and total RAC1. 

25389450 31314174
In vivo Treatment of KITD814V-bearing cells with EHop-016 significantly enhances the survival of leukemic mice. [2]


Kinase Assay:[1]
- Collapse

Rac Activity Assays:

Rac activity is determined from lysates of the MDA-MB-435 and MDA-MB-231 human metastatic cancer cell lines (from ATCC). Cancer cells in culture medium (DMEM, 10% FBS, pH 7.5) are treated with vehicle (0.1% DMSO) or varying concentrations of EHop-016 (0–10 μM) for 24 h. Rac1 activity is determined using the G-LISA Rac1 activation assay kit.
Cell Research:[1]
- Collapse
  • Cell lines: MDA-MB-231, MDA-MB-435, or MCF-10A cells
  • Concentrations: ~10 μM
  • Incubation Time: 24 hours
  • Method: MDA-MB-231, MDA-MB-435, or MCF-10A mammary epithelial cells (from ATCC) are incubated in vehicle (0.1% DMSO) or varying concentrations of EHop-016 (0–10 μM) for 24 h. Cell viability is measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell survival and proliferation kit according to the manufacturer's instructions.
    (Only for Reference)
Animal Research:[2]
- Collapse
  • Animal Models: KITD814V-bearing mice.
  • Dosages: 2.5 μM
  • Administration: KITD814V-bearing 32D cells with EHop-016 are administered by i.v. injection.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 86 mg/mL (199.74 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 430.55


CAS No. 1380432-32-5
Storage powder
in solvent
Synonyms N/A
Smiles CC[N]1C2=C(C=CC=C2)C3=C1C=CC(=C3)NC4=NC(=NC=C4)NCCCN5CCOCC5

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation (Different batches have different solubility ratios, please contact Selleck to provide you with the correct ratio)
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Rho Signaling Pathway Map

Tags: buy EHop-016 | EHop-016 supplier | purchase EHop-016 | EHop-016 cost | EHop-016 manufacturer | order EHop-016 | EHop-016 distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID