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CCG-1423 Rho inhibitor

Name: CCG-1423
Brand: Selleck Chemicals
SKU: S7719
Availability: InStock
Rating: 5 (38 reviews)

Cat.No.S7719

CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

Chemical Structure

Molecular Weight: 454.75

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S771901 DMSO]90 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99.98%

99.98

Related Targets	DNA/RNA Synthesis CDK Microtubule Associated Ras HSP PD-1/PD-L1 Aurora Kinase Casein Kinase Serine/threonin kinase eIF Chk ROCK Myc Kinesin PLK DHFR PAK PERK DYRK RUNX PP2A PFKFB AP-1 MPS1 Wee1 p21 BMI-1 LIM kinase Nur77 APC
Related Products	NSC 23766 trihydrochloride EHop-016 ML141 EHT 1864 2HCl ZCL278 MBQ-167 CCG-203971 Rhosin hydrochloride CID44216842 RhoGDI Antibody [H2N24] CASIN CDC42 Antibody [B4N7] TC10 Antibody [D5M19] CCG-100602 SRF Antibody [N16D21] CCG-222740 Rho A Antibody [G4B13] RP-102124 Rho Antibody [B24D19] Rac1 Antibody [D22N1] MLS-573151 RhoE Antibody [A21D22] Phospho-VAV1 (Tyr174) Antibody [K4A21] 1A-116 SAR407899 hydrochloride Rac1 + Rac2 + Rac3 Antibody [G9P16] MLS000532223 VAV2 Antibody [B11L17] RhoA Antibody [B21G13] Y16

Chemical Information, Storage & Stability

Molecular Weight	454.75	Formula	C₁₈H₁₃ClF₆N₂O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	285986-88-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(C(=O)NC1=CC=C(C=C1)Cl)ONC(=O)C2=CC(=CC(=C2)C(F)(F)F)C(F)(F)F

Solubility

In vitro
Batch:

DMSO : 90 mg/mL (197.91 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.500mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 90 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.560mg/ml (1.23mM)	Taking the 1 mL working solution as an example, add 50 μL of 11.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	RhoA ^[1]
In vitro	CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. This compound also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. ^[1] It in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. ^[2] In H9c2 cells, this chemical inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. ^[3] This compound, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. ^[4]
References	[1] https://pubmed.ncbi.nlm.nih.gov/17699722/ [2] https://pubmed.ncbi.nlm.nih.gov/20171952/ [3] https://pubmed.ncbi.nlm.nih.gov/22815879/ [4] https://pubmed.ncbi.nlm.nih.gov/24280883/

Tech Support

Handling Instructions

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