CCG-1423

Catalog No.S7719

CCG-1423 Chemical Structure

Molecular Weight(MW): 454.75

CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.

Size Price Stock Quantity  
USD 97 In stock
USD 247 In stock
USD 647 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 5 Publications

5 Customer Reviews

  • Cells were treated with CCG-1423 for 48 h. CCG-1423 blocked the nuclear translocation of MRTF-A.

    Cell Signal, 2017, 31:87-95. CCG-1423 purchased from Selleck.

    CCG-1423 blocked the nuclear translocation of MRTF-A.

    Cellular Signalling, 2017, 31:87-95.. CCG-1423 purchased from Selleck.

  • The expression of type I collagen was decreased by a selective MRTF-A inhibitor CCG-1423. (A, C and E) CCG-1423 inhibited the synthesis COL1A1 mRNA in MCF-7, T47D and MDA-MB-231 cells. (B, D and F) CCG-1423 inhibited the synthesis of type I collagen in MCF-7, T47D and MDA-MB-231 cells. From A to F, breast cancer cells were treated with 10 μM of CCG-1423 for 24 h. mRNAs and proteins were examined with RT-qPCR or Western-blot. (G) CCG-1423 inhibited COL1A1 promoter activity in MCF-7 breast cancer cells. Data are shown as mean ± S.E.M, based on three to six independent experiments. **, p < 0.01.

    Biomed Pharmacother, 2018, 104:718-728. CCG-1423 purchased from Selleck.

    Daam1 and RhoA participates in the rearrangement of stress fibers in glioblastoma cells. Daam1 siRNA and CCG-1423 disrupted the formation of actin stress fibers in U251 cells. U251 cells were grown on coverslips, then transiently transfected with Daam1 siRNA or pre-treated with 10 ng/ml CCG-1423 for 1 h. U251 cells were then incubated in culture medium containing 20 ng/ml recombinant Wnt5a (rWnt5a) for 4 h. Subsequently, the cells were fixed and F-actin organization was analyzed by phalloidin staining. Magnification, ×400.

    Oncol Rep, 2018, 39(2):465-472. CCG-1423 purchased from Selleck.

  • QKI-silenced CAKI-1 cells were cultured in medium with/without 1.5 μM CCG-1423 (RhoA inhibitor) for 24 h. Levels of YAP and pYAP expression were detected by western blot analysis. The relative protein band intensity was quantified by densitometry and normalized to β-actin using Image J analysis software. Each experiment was repeated three times and data are presented as the mean ± SD. *P < 0.05 for inhibitor− vs. inhibitor+.

    Acta Biochim Biophys Sin, 2018, doi:10.1093/abbs/gmy142. CCG-1423 purchased from Selleck.

Purity & Quality Control

Choose Selective Rho Inhibitors

Biological Activity

Description CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.
Targets
RhoA [1]
In vitro

CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. CCG-1423 also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. [1] CCG-1423 in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. [2] In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. [3] CCG-1423, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: Melanoma lines that overexpress RhoC (A375M2 and SK-Mel-147), low RhoC-expressing lines (A375 and SK-Mel-28), transformed (SW962, PC-3, and SKOV-3) and nontransformed (WI-38) cell lines.
  • Concentrations: 300 nM
  • Incubation Time: 72 hours
  • Method: Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 90 mg/mL (197.91 mM)
Ethanol 4 mg/mL warmed (8.79 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 454.75
Formula

C18H13ClF6N2O3

CAS No. 285986-88-1
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Rho Signaling Pathway Map

Tags: buy CCG-1423 | CCG-1423 supplier | purchase CCG-1423 | CCG-1423 cost | CCG-1423 manufacturer | order CCG-1423 | CCG-1423 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID