NSC 23766

Catalog No.S8031

NSC 23766 Chemical Structure

Molecular Weight(MW): 530.96

NSC 23766 is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM in a cell-free assay; does not inhibit the closely related targets, Cdc42 or RhoA.

Size Price Stock Quantity  
In DMSO USD 140 In stock
USD 110 In stock
USD 470 In stock
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Cited by 10 Publications

6 Customer Reviews

  • MES cells were treated with Rac inhibitor (50 μM EHT1864 or NSC23766) and assayed for self-renewal by serial passage mammosphere formation. P1, passage 1; P2, passage 2. Data are means ± SEM of three biological replicates. *P ≤ 0.05 by two-tailed t test.

    Science, 2018, 11(528), doi: 10.1126/scisignal.aao6897. NSC 23766 purchased from Selleck.

    Liver samples from both groups treated with olive oil, CCl4, CCl4+NSC23766 or +EHop-016 for 4 weeks were collected for Rac1 activity assay and western blot analysis.

    Cell Death Dis, 2015, 6: e1751 . NSC 23766 purchased from Selleck.

  • IPEC-J2 cells were treated with PBS, 20 μg/ml CWA, 50 μM NSC 23766 (specific Rac1 inhibitor), or 20 μg/ml CWA with 50 μM NSC 23766 for 12 h. (C) Then, the IPEC-J2 cells were collected for Western blot analysis. (D) Alternatively, after washing with D-Hanks'solution, the IPEC-J2 cells were infected with 1 × 106 CFU of EHEC O157:H7 for 1 h and then washed with PBS. The cells were then collected for colony counting.

    J Immunol, 2017, 198(4):1696-1705. NSC 23766 purchased from Selleck.

    Effects of inhibitor of Rac1 on regulating NF-κB, PARP-1, p-AMPK andp-p70S6K in GFP-ART1 CT26 cells under starvation-induced conditions. The expressions of PARP-1 and p-AMPK reduce and the expression of p-p70S6K increase in the GFP-ART1 CT26 cells which treated with NSC23766.

    Am J Cancer Res, 2015, 5(2): 498-513. NSC 23766 purchased from Selleck.

  • Effects of PMA and radiation on the subcellular distribution of Rac1 in CNE-1 cells. After different treatments, CNE-1 cells were immediately washed with PBS and fixed for immunofluorescence analysis under a confocal microscope. The red arrows indicate where Rac1 was recruited to the protruding edge of CNE-1 cells. The nuclei are stained blue by DAPI and the Rac1 protein are indicated in green. Original magnification 600×, scale bar=50 μm.

    Radiat Res, 2016, 185(6):638-46. . NSC 23766 purchased from Selleck.

    SH3BP1 activates Rac1 to promote Wave2 expression in cervical cancer cells (A) and (C) Rac1 activity in HeLa and Caski cells in response to co-processing SH3BP1 overexpression and Rac1 inhibitor NSC 23766 was determined using GST-pull down assays. (B) and (D) Wave2 protein levels in HeLa and Caski cells in response to co-processing SH3BP1 overexpression and Rac1 inhibitor NSC 23766 were determined using Western blot assays. The data are presented as mean ± SD of three independent experiments. *P<0.05, **P<0.01, compared to SH3BP1 (-) + NSC 23766 (-) group; ##P<0.01, compared to SH3BP1 (+) + NSC 23766 (-) group.

    J Cell Biochem, 2018, 119(2):1733-1745. NSC 23766 purchased from Selleck.

Purity & Quality Control

Choose Selective Rho Inhibitors

Biological Activity

Description NSC 23766 is an inhibitor of Rac GTPase targeting Rac activation by guanine nucleotide exchange factors (GEFs) with IC50 of ~50 μM in a cell-free assay; does not inhibit the closely related targets, Cdc42 or RhoA.
Targets
Rac GTPase [1]
(Cell-free assay)
50 μM
In vitro

NSC23766 is identified to fit into a surface groove of Rac1 known to be critical for GEF specification. NSC23766 effectively inhibits Rac1 binding and activation by the Rac-specific GEF Trio or Tiam1 in a dose-dependent manner without interfering with the closely related Cdc42 or RhoA binding or activation by their respective GEFs or with Rac1 interaction with BcrGAP or effector PAK1. [1] NSC 23766 is active in regulating Rac GTPase functions on cytoskeleton and many cell functions including cell cycle, cell growth, adhesion, migration and gene transcription. NSC 23766 (50 μM) potently blocks serum or platelet-derived growth factor-induced Rac1 activation and lamellipodia formation without affecting the activity of endogenous Cdc42 or RhoA in NIH 3T3 cells. NSC 23766 reduces Trio or Tiam1 but not Vav, Lbc, Intersectin, or a constitutively active Rac1 mutant-stimulated NIH 3T3 cells growth and suppresses Trio, Tiam1, or Ras-induced cell transformation. NSC23766 dose-dependently inhibits PC-3 cells proliferation and anchorage-independent growth. 25 μM NSC23766 inhibits the PC-3 cell invasion through Matrigel by 85%. [1] 50 μM NSC 23766 inhibits thrombin-induced activation of Rac1 an d Rac2 in human platelets, as well as platelet aggregation. [2] NSC23766 prevents Aβ40 and Aβ42 production in swAPP-HEK293cells without affecting Notch and sAPPα. NSC23766 prevents γ-secretase activity in cell, but not act as a direct γ-secretase inhibitor. NSC23766 dose-dependently reduces levels of secreted and intracellular Aβ40 with IC50 of 48.94 μM. 50 μM NSC 23766 inhibits release of Aβ42 by 57.97%. [3] NSC23766 regulates endothelial nitric oxide synthase expression and endothelial function. 100 μM NSC23766 represses the eNOS promoter activity by 60% in bovine aortic ECs and by 30% to 35% in bEND.3 cells. Inhibition of Rac1 with NSC23766 destabilizes eNOS mRNA and shortens its half-life to 17 hours. NSC23766 dose-dependently attenuates ACh-induced relaxation of wild-type mice aortic rings. [4] NSC23766 inhibits cell growth and induces apoptosis. NSC23766 decreases MDA-MB-468 and MDA-MB-231 cells viability in a dose-dependent manner with IC50 of ~10 μM, which is not correlated with the status of estrogen receptor (ER), progesterone receptor (PR), Her2, and p53 mutation. NSC23766 has little effect on the survival of the MCF12A normal mammary epithelial cells. After 24 hours expose to NSC 23766, MDA-MB-231 cells showes an increase from 41% to 65% in G1 phase and a concomitant decrease in S and G2-M phases. 100 μM NSC23766 induces a six-fold increase of apoptotic MDA-MB-468. The inhibition of NSC23766 on cell cycle arrest or apoptosis in breast cancer cells is mediated by downregulation of cyclin D1, survivin, and X-linked inhibitor of protein apoptosis. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RBMECs MmTjSpVv[3Srb36gRZN{[Xl? MX:xNFDDqM7:TdMg NIf2e5I{OMLibXnu MV;icI9kc2W|IE\Ccpou[0GPUD3t[YRq[XSnZDDhZ5RqfmG2aX;uJI9nKFKjY{GgbY4hTU2DUD3JTU11emWjdHXkJHJDVUWFcx?= NHfpPIszPjN3OECzPS=>
A431 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Xsc|ExOCEQvF2= NH7lXJkzPC92OD:3NkBp MkTZbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> MnTBNlUyODl|Mke=
SW480  NGLTOXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3KSYYyODBizszN NWT6cXBrOjRxNEivO|IhcA>? MnPUbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> NV7lWFJwOjVzMEmzNlc>
U2-OS MkT6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\4UGwyODBizszN MmSyNlQwPDhxN{KgbC=> M2nTNIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGgeIlu\SCmZYDlcoRmdnRibXHucoVz M3ziSVI2OTB7M{K3
A431 NW\YNWZ7TnWwY4Tpc44hSXO|YYm= NHXVTXgyODBizszN MWmyOEBp M{XGbGROW09? NVzwcHRCcW6mdXPld{Bk\WyuIHP5Z4xmKGG{cnXzeEBqdiC2aHWgS|EheGijc3ZCpC=> MUCyOVExQTN{Nx?=
SW480  NU\3OI54TnWwY4Tpc44hSXO|YYm= M3;VZlExOCEQvF2= NVXCUHg4OjRiaB?= MXnEUXNQ M{\Ke4lv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgbY4hfGinIFexJJBp[XOnwrC= NVHYXGFOOjVzMEmzNlc>
U2-OS M1vqdWZ2dmO2aX;uJGF{e2G7 M2fyNFExOCEQvF2= M{HPflI1KGh? M1O0cWROW09? Mlf6bY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dDDpckB1cGViR{GgdIhie2YEoB?= NF3pfpQzPTFyOUOyOy=>
NIH3T3  NI\oXI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\meVExOCEQvF2= NXTMTYViOjUEoHi= M2r3[YhieyCwbzDzbYdvcW[rY3HueEBqdXCjY4Sgc44h[2WubDD2bYFjcWyrdIm= MUWyOVA{PzB4MB?=
Ki-67+ CLL M4\leGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnu5OVAhyrWP NGfCOWo2KGR? NIfoVnVl\WO{ZXHz[ZMhfGinIH71cYJmeiCxZjDLbU03PywEoFPMUEBk\Wyucx?= M1SwSFI1PTBzMkG3
U87MG MmHpR4VtdCCYaXHibYxqfHliQYPzZZk> MUm1NEBuVQ>? NGnHfWoyPDRiaB?= M4HybGROW09? M3PCOYV5cGmkaYTzJJN6dmW{Z3nzeIlkKGGwdHnwdo9tcW[ncnH0bZZmKGWoZnXjeJMh[2:vYnnu[YQhfHKnYYTt[Y51KHerdHig[ZJtd3SrbnnixsA> NYfHWmZqOjN6M{KxNlA>
A172MG MmK5R4VtdCCYaXHibYxqfHliQYPzZZk> M{\zOFUxKG2P MmizNVQ1KGh? MkO4SG1UVw>? Momw[ZhpcWKrdIOgd5lv\XKpaYP0bYMh[W62aYDyc4xq\mW{YYTpeoUh\W[oZXP0d{Bkd22kaX7l[EB1emWjdH3lcpQhf2m2aDDldoxwfGmwaXNCpC=> M1vvb|I{QDN{MUKw
T98MG NYPqeW5yS2WubDDWbYFjcWyrdImgRZN{[Xl? NWnWdJZFPTBibV2= NUDhN2h[OTR2IHi= NWn6cphqTE2VTx?= NUi5bWpS\XiqaXLpeJMhe3mwZYLnbZN1cWNiYX70bZBzd2yrZnXyZZRqfmViZX\m[YN1eyClb33ibY5m\CC2cnXheI1mdnRid3n0bEBmemyxdHnubYLDqA>? M{L6VVI{QDN{MUKw
PC38 Mn;kR4VtdCCYaXHibYxqfHliQYPzZZk> NELoNJY2OCCvTR?= NH:wTVQyPDRiaB?= MVnEUXNQ NEPpboRmgGirYnn0d{B{gW6ncnfpd5Rq[yCjboTpdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGOxbXLpcoVlKHS{ZXH0cYVvfCC5aYToJIVzdG:2aX7pZuKh NIjvSJczOzh|MkGyNC=>
PC40 NF7YOXRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MluzOVAhdU1? MmL2NVQ1KGh? MXLEUXNQ M2TaToV5cGmkaYTzJJN6dmW{Z3nzeIlkKGGwdHnwdo9tcW[ncnH0bZZmKGWoZnXjeJMh[2:vYnnu[YQhfHKnYYTt[Y51KHerdHig[ZJtd3SrbnnixsA> NX:3OJZVOjN6M{KxNlA>
U87MG NH73ZoFHfW6ldHnvckBCe3OjeR?= M4njWlUxKG2P M1TWTlI1KGh? M{LwbWROW09? M4P3R4VvcGGwY3XzJJRp\SCjboTpcYloemG2b4L5JIVn\mWldDDv[kBmemyxdHnubYI> NXTscYIyOjN6M{KxNlA>
A172MG NEPyNWRHfW6ldHnvckBCe3OjeR?= M1;GNVUxKG2P M{flb|I1KGh? NVuyOJNmTE2VTx?= M{eze4VvcGGwY3XzJJRp\SCjboTpcYloemG2b4L5JIVn\mWldDDv[kBmemyxdHnubYI> NGGxUZozOzh|MkGyNC=>
T98MG NVjyNIFyTnWwY4Tpc44hSXO|YYm= MVe1NEBuVQ>? NELnTJEzPCCq MYfEUXNQ M1fXR4VvcGGwY3XzJJRp\SCjboTpcYloemG2b4L5JIVn\mWldDDv[kBmemyxdHnubYI> M3vx[|I{QDN{MUKw
NCI-H1703 NHzudINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7GS2UxNTVyMDFOwG0> NHLme|MzPCCq M4[2c4lvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\SCmZYDlcoRmdnRibXHucoVz NGHxVlIzOjV2OUG2NC=>
NCI-H1703 MYPGeY5kfGmxbjDBd5NigQ>? MY[xNFAh|rypL33s NEHKSFEzPCCq NULWTYl1e2yxd4OgdJJw\3Knc4Ppc44hfGi{b4XnbEB1cGViR{JCpJBp[XOnIH;mJJRp\SClZXzsJIN6[2yn NWW4Rm1NOjJ3NEmxOlA>
NCI-H1703 MlqySpVv[3Srb36gRZN{[Xl? NGPTXWcxNTVyMDFOwG0> M1fscFI1KGh? M2HiN4RqdWmwaYPo[ZMh[mG|YXygUmYu|rqEIHHjeIl3cXS7IHTvd4Uh\GWyZX7k[Y51dHoEoB?= MV:yNlU1QTF4MB?=
SKBR3 MUPGeY5kfGmxbjDBd5NigQ>? NHTRbpM2OCEQvF2= NVftc2hvOjRiaB?= Mo\abY5pcWKrdIOgVoFkOSCjY4TpeoF1cW:w MmjLNlE6PDN6MkW=
SKBR3-pMKO.1 MXzGeY5kfGmxbjDBd5NigQ>? Ml3vOVAh|ryP NFjSTI4zPCCq MlLnbY5pcWKrdIOgVoFkOSCjY4TpeoF1cW:w MoG1NlE6PDN6MkW=
MCF7 M2rtOmN6fG:2b4jpZ4l1gSCDc4PhfS=> NUXkTnp4OC1zMECg{txO MoPjOFghcA>? MY\k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MoTGNlA2OTV7NEC=
T47D MUPDfZRwfG:6aXPpeJkhSXO|YYm= MWCwMVExOCEQvF2= NVHWZ|FyPDhiaB?= MV3k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? MoXvNlA2OTV7NEC=
MDA-MB-468 Ml\1R5l1d3SxeHnjbZR6KEG|c3H5 MkewNE0yODBizszN NV;XcW5pPDhiaB?= M2XoOoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= NVTISlNiOjB3MUW5OFA>
MDA-MB-231 MYfDfZRwfG:6aXPpeJkhSXO|YYm= NW[wcVhNOC1zMECg{txO M1\EcVQ5KGh? NF;m[npl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NYDjU2tTOjB3MUW5OFA>
MDA-MB-231 MmDzSpVv[3Srb36gRZN{[Xl? NF\oWYoxNTFyMDFOwG0> MmL5NlQhcA>? M{LTfJNmdGWldHn2[Yx6KGmwaHnibZR{KFKjY{GgZYN1cX[jdHnvckB4cXSqb4X0JIlvfGW{ZnXybY5oKHerdHigeIhmKGGldHn2bZR6KG:oIITo[UBkdG:|ZXz5JJJmdGG2ZXSgd41idGxiR2TQZZNmKEOmY{Sy NHfrUY8zODVzNUm0NC=>
MDA-MB-231  MWnGeY5kfGmxbjDBd5NigQ>? M4T1S|ExOCEQvF2= NVXiVFJSPDhiaB?= MULpcoNz\WG|ZYOgeIhmKGOnbHygcpVu[mW{IHnuJGcyyqCyaHHz[UBidmRiZHXjdoVie2W|IITo[UBk\WyuIH71cYJmeiCrbjDTJIFv\CCJMj3NJJBp[XOnc9Mg MmTPNlA2OTV7NEC=
MCF7 MVnGeY5kfGmxbjDBd5NigQ>? MkP4NVAxKM7:TR?= M3ToU|Q5KGh? NWi4TI5JcW6lcnXhd4V{KHSqZTDj[YxtKG63bXLldkBqdiCJMdMgdIhie2ViYX7kJIRm[3KnYYPld{B1cGViY3XscEBvfW2kZYKgbY4hWyCjbnSgS|IuVSCyaHHz[ZPDqA>? MXGyNFUyPTl2MB?=
T47D M1TNPGZ2dmO2aX;uJGF{e2G7 Mor1NVAxKM7:TR?= NH2zTGw1QCCq M{nYdYlv[3KnYYPld{B1cGViY3XscEBvfW2kZYKgbY4hTzIEoIDoZZNmKGGwZDDk[YNz\WG|ZYOgeIhmKGOnbHygcpVu[mW{IHnuJHMh[W6mIFeyMW0heGijc3XzxsA> NVjRbZNROjB3MUW5OFA>
MDA-MB-468 Mmi0RZBweHSxc3nzJGF{e2G7 NEPQbVA2OC9zMECg{txO M3f4XFI1KGh? NUfZbnQzcW6mdXPld{BieG:ydH;zbZM> NYSwW4w{OjB3MUW5OFA>
MDA-MB-468 NG\5NIlHfW6ldHnvckBCe3OjeR?= NVj3O|QxOTByIN88US=> NVjNcHpMOjRiaB?= MUTpcohq[mm2c9MgZ4F{eGG|ZT2zJIFkfGm4YYTpc47DqA>? M17HPFIxPTF3OUSw
MDA-MB-468 NYPFbZpGTnWwY4Tpc44hSXO|YYm= NX3SfJFpPTBxMUCwJO69VQ>? NFrrS2ozPCCq M4nmSYlv[3KnYYPld{BxcG:|cHjvdplt[XSrb36gc4YhUk6NIHnuJIEh\G:|ZTDk[ZBmdmSnboSgcYFvdmW{ M1Lq[|IxPTF3OUSw
MDA-MB-231  MYLGeY5kfGmxbjDBd5NigQ>? MnL5OVAwOTByIN88US=> NIO3cGozPCCq MULpcoNz\WG|ZYOgdIhwe3Cqb4L5cIF1cW:wIH;mJGpPUyCrbjDhJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? MXmyNFUyPTl2MB?=
MDA-MB-468 NHzYNIpHfW6ldHnvckBCe3OjeR?= M1:3SFUxNzFyMDFOwG0> M{[wcVQ5KGh? NI\4[JBqdmS3Y3XzJIEh\G:|ZT3k[ZBmdmSnboSg[IVkemWjc3WgbY4heGixc4Doc5J6dGG2aX;uJI9nKHB4NTDzeYJ2dmm2 M3\r[|IxPTF3OUSw
MDA-MB-231  NYG2Um5ITnWwY4Tpc44hSXO|YYm= NVf5ZXc3PTBxMUCwJO69VQ>? NU\VRZhJPDhiaB?= NUTseGc3cW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JIRm[3KnYYPlJIlvKHCqb4PwbI9zgWyjdHnvckBw\iCyNkWgd5VjfW6rdB?= MUOyNFUyPTl2MB?=
IEC-6  M3vPPWZ2dmO2aX;uJGF{e2G7 MnzCNVIxKML3TR?= NHn1eJc1NzZxODDo NGjyOZRxemW4ZX70d{B1cGViaX7jdoVie2WmIHHjeIl3[XSrb36gc4YhTkGNIHH0JFYh[W6mIEigbC=> NWfGe2JFOjB2NEi0OlE>
RA-FLS (RA2)  M3q2Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHP3T3kzPS93MDFOwG0> NXXtRotzOS17IHS= M3L1OYlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHLveIgh\G:|ZTDhcoQhfGmvZTDk[ZBmdmSnboSgcYFvdmW{ M{SzeVE4PjJ{M{C4
RA1 M125WmZ2dmO2aX;uJGF{e2G7 NGLvWoQ2OCEQvF2= MUGyOEBp M3XhVIlvcGmkaYTzJG1ifHKrZ3XsJIlvfmG|aX;u MlfjNVc3OjJ|MEi=
RA2 MWXGeY5kfGmxbjDBd5NigQ>? NWTOR|hWPTBizszN MkDaNlQhcA>? MkG3bY5pcWKrdIOgUYF1emmpZXygbY53[XOrb36= M{n2VFE4PjJ{M{C4
RA3 MWHGeY5kfGmxbjDBd5NigQ>? NGnxR|I2OCEQvF2= NEXVbnMzPCCq NFrsO4hqdmirYnn0d{BO[XS{aXflcEBqdn[jc3nvci=> NF;PcmsyPzZ{MkOwPC=>
RA4 NXTqZ4xiTnWwY4Tpc44hSXO|YYm= MUC1NEDPxE1? MXKyOEBp NXTZZpZPcW6qaXLpeJMhVWG2cnnn[YwhcW64YYPpc44> NYrDPINoOTd4MkKzNFg>

... Click to View More Cell Line Experimental Data

In vivo NSC23766 induces mobilization of hematopoietic stem cells/progenitors. Intraperitoneal administration of NSC23766 (2.5 mg/kg) into the ‘‘poorly mobilizing ’’ C57Bl/6 mouse strain leads to a two-fold increase in circulating hematopoietic stem cells/progenitors 6 hr after injection. [2] NSC23766 alleviates lipopolysaccharide-induced acute pulmonary injury in mice. Treatment with NSC23766 at 1 or 3mg/kg not only reduces the inflammatory cells infiltration and MPO activities, but also inhibits pro-inflammatory mediators, tumor necrosis factor-α and interleukin-1β, mRNA expression. NSC23766 also reduces Evans Blue and albumin accumulation in LPS-challenged lungs. [6]

Protocol

Kinase Assay:[1]
+ Expand

Rho GTPase activity assay:

Cells are grown in log phase in a 10-cm dish, and are starved in 0.5% serum medium or indicated otherwise for 24 h before lysis in a buffer containing 20 mM Tris HCl (pH 7.6), 100 mM NaCl, 10 mM MgCl2, 1% Nonidet P-40, 10% glycerol, and 1× protease inhibitor mixture. Lysates are clarified, the protein concentrations are normalized, and the GTP-bound Rac1 in the lysates is measured by an effector domain pull-down assay. For the His6-PAK1 PBD pull-down assay, cell lysates are incubated with Ni2+-agarose-immobilized His6-PAK1 PBD domain (∼1 μg each) purified from E. coli for 30 min. The Ni2+-agarose co-precipitates are washed twice in the wash buffer and analyzed by immunoblotting with anti-Rac1 monoclonal antibody.
Cell Research:[5]
+ Expand
  • Cell lines: Human breast cancer cells MDA-MB-468
  • Concentrations: 0-100 μM
  • Incubation Time: 2 days
  • Method: Cells (1.5 × 104/mL) are seeded in each well of 96-well tissue culture plates with 200 μL of medium. After 24 hours of plating, the medium is replaced with 200 μL of fresh medium containing NSC23766 at the indicated concentrations. At the end of the treatment period 20 μL of MTS solution are added to each well and incubated at 37 ℃ for 2 hours. Absorbance at 490 nm is read on a 96-well plate reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (188.33 mM)
Water 100 mg/mL warmed (188.33 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 530.96
Formula

C24H35N7.3HCl

CAS No. 1177865-17-6
Storage powder
in solvent
Synonyms N/A

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    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Rho Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID