Cabazitaxel
For research use only.
Catalog No.S3022 Synonyms: RPR-116258A, XRP6258, TXD 258
17 publications
Molecular Weight(MW): 835.93
Cabazitaxel (RPR-116258A, XRP6258, TXD 258) is a semi-synthetic derivative of a natural taxoid that kills cancer cells by inhibiting cell division and growth. Cabazitaxel exerts its effects by inhibiting microtubule growth and assembly, processes that are essential for cells to divide. Cabazitaxel induces autophagy via the PI3K/Akt/mTOR pathway.
5 Customer Reviews
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Quantification of colony formation assays of aggressive PC cells (DU145-DR and 22Rv1-DR) transfected with control siRNA or two siRNAs targeting each specific nucleoporin following 72 hour treatment with cabazitaxel (25 nM) and single dose radiation (5 Gys).
Cell, 2018, 174(5):1200-1215. Cabazitaxel purchased from Selleck.
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ABCB1 functional inhibition using elacridar re-sensitizes TaxR and DU145-DTXR cells to cabazitaxel treatment. A. TaxR and DU145-DTXR cells were subjected to cell growth assays using either vehicle (DMSO), elacridar (0.5μM), cabazitaxel (1nM), or a combination of elacridar and cabazitaxel. Cells were counted 72 hours post-treatment. B. TaxR and DU145-DTXR cells were treated with either vehicle (DMSO), elacridar (0.5μM), cabazitaxel (1nM), or a combination of both elacridar and cabazitaxel for 72 hours. Whole cell lysates were then prepared and subjected to western blot analysis using indicated antibodies. Tubulin served as a loading control. c-PARP = cleaved-PARP, C = control (DMSO) treatment, Elac = elacridar, CTX = cabazitaxel. All data is presented as percent of control mean ± standard deviation. * = p-value ≤ 0.05.
Mol Cancer Ther, 2017, 16(10):2257-2266. Cabazitaxel purchased from Selleck.
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Viability of PC-3R and PC-3 cells treated with docetaxel and cabazitaxel. The 3,000 cells seeded in a 96-well plate were treated with docetaxel or cabazitaxel at indicated concentrations for 72 hours and subjected to WST assay (n=5).
Urol Oncol, 2015, 33(9):385.e15-20.. Cabazitaxel purchased from Selleck.
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Immunoblot showing FKBP7 protein level in various parental cells after treatment with 10 nmol/L docetaxel or 3 nmol/L cabazitaxel for 24, 48, 72, and 120 hours. Actin or Hsc70 are the loading control.s
Clin Cancer Res, 2018, doi:10.1158/1078-0432.CCR-18-0704. Cabazitaxel purchased from Selleck.
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Combination effect of cabazitaxel with drugs that target the PI3K-pathway simultaneously: LNCaP cells were treated with the indicated combination of the drugs and analyzed as described in Methods and Figure Figure3
Oncotarget, 2016, 7(46):76181-76196. Cabazitaxel purchased from Selleck.
Purity & Quality Control
Choose Selective Microtubule Associated Inhibitors
Biological Activity
| Description | Cabazitaxel (RPR-116258A, XRP6258, TXD 258) is a semi-synthetic derivative of a natural taxoid that kills cancer cells by inhibiting cell division and growth. Cabazitaxel exerts its effects by inhibiting microtubule growth and assembly, processes that are essential for cells to divide. Cabazitaxel induces autophagy via the PI3K/Akt/mTOR pathway. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Features | A semi-synthetic derivative of a natural taxoid. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Targets |
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| In vitro |
Cabazitaxel increases CYP3A enzyme activities in rat hepatocytes. The mean ex-vivo human plasma protein binding of Cabazitaxel is 91.6%. Cabazitaxel is rapidly and extensively metabolised in numerous metabolites. Cabazitaxel demonstrates activity in several murine and human resistant cell lines. [1] With a 4-day exposure to cabazitaxel, cytotoxicity is noted with relatively low cabazitaxel concentrations. Cabazitaxel shows high antitumor activity in 3 human colorectal cell lines (HCT-116, HCT-8, and HT-29). [2] |
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| Cell Data |
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| In vivo | In accompanying models, Cabazitaxel is noted to have significant antitumor activity. In murine tumor xenografts (colon C38 and pancreas P03), Cabazitaxel elicites complete tumor regressions. Using SF-295 and U251 human glioblastoma cell lines, both orthotopic and subcutaneous murine xenografts are generated. Cabazitaxel treatment leads to complete regression in the majority of subcutaneously implanted tumors. Furthermore, in orthotopic models, Cabazitaxel leads to complete tumor regression in 4 out of 10 U251 tumors. [2] |
Protocol
Solubility (25°C)
| In vitro | DMSO | 100 mg/mL (119.62 mM) |
|---|---|---|
| Water | Insoluble | |
| Ethanol | Insoluble |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 835.93 |
|---|---|
| Formula | C45H57NO14 |
| CAS No. | 183133-96-2 |
| Storage |
powder in solvent |
| Synonyms | RPR-116258A, XRP6258, TXD 258 |
| Smiles | CC1=C2C(C(=O)C3(C(CC4C(C3C(C(C2(C)C)(CC1OC(=O)C(C(C5=CC=CC=C5)NC(=O)OC(C)(C)C)O)O)OC(=O)C6=CC=CC=C6)(CO4)OC(=O)C)OC)C)OC |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Bio Calculators
Molarity Calculator
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Dilution Calculator
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Molarity Calculator
Clinical Trial Information
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|---|
| NCT04495179 | Not yet recruiting | Drug: AZD4635|Drug: Durvalumab|Drug: Cabazitaxel | Progressive Metastatic Castrate-Resistant Prostate Cancer | AstraZeneca|Parexel | July 24 2020 | Phase 2 |
| NCT03257891 | Recruiting | Drug: Cabazitaxel | Adrenocortical Carcinoma | Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia|San Luigi Gonzaga Hospital | January 25 2018 | Phase 2 |
| NCT02844582 | Completed | Drug: Cabazitaxel|Drug: Prednisone | Hormone-Resistant Prostate Cancer|Stage IV Prostate Adenocarcinoma | University of California Davis|Sanofi | December 20 2017 | Phase 2 |
| NCT03043989 | Terminated | Drug: Docetaxel|Drug: Cabazitaxel|Drug: Clarithromycin | Prostate Cancer | Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|Maryland Technology Development Corporation | March 21 2017 | Phase 1 |
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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Frequently Asked Questions
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Question 1:
What is the elimination half-life of cabazitaxel?
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Answer:
According to the paper report, the elimination half-life of cabazitaxel is 95h.
