Vemurafenib (PLX4032)

Catalog No.S1267 Synonyms: RG7204, RO5185426

For research use only.

Vemurafenib (PLX4032, RG7204, RO5185426) is a novel and potent inhibitor of B-RafV600E with IC50 of 31 nM in cell-free assay. 10-fold selective for B-RafV600E over wild-type B-Raf in enzymatic assays and the cellular selectivity can exceed 100-fold. Vemurafenib (PLX4032, RG7204) induces autophagy.

Vemurafenib (PLX4032) Chemical Structure

CAS No. 918504-65-1

Selleck's Vemurafenib (PLX4032) has been cited by 554 publications

Purity & Quality Control

Choose Selective Raf Inhibitors

Other Raf Products

Biological Activity

Description Vemurafenib (PLX4032, RG7204, RO5185426) is a novel and potent inhibitor of B-RafV600E with IC50 of 31 nM in cell-free assay. 10-fold selective for B-RafV600E over wild-type B-Raf in enzymatic assays and the cellular selectivity can exceed 100-fold. Vemurafenib (PLX4032, RG7204) induces autophagy.
Features A novel and potent inhibitor of the B-RAFV600E oncoprotein.
Targets
SRMS [1]
(Cell-free assay)
ACK1 [1]
(Cell-free assay)
B-Raf (V600E) [1]
(Cell-free assay)
C-Raf [1]
(Cell-free assay)
MAP4K5 (KHS1) [1]
(Cell-free assay)
Click to View More Targets
18 nM 19 nM 31 nM 48 nM 51 nM
In vitro

PLX4032 inhibits B-RAFV600E, C-RAF, as well as wildtype B-RAF, with IC50 of 31 nM, 48 nM and 100 nM, respectively. PLX4032 also inhibits several non-RAF kinases, including ACK1, KHS1, and SRMS, with IC50 of 18 nM to 51 nM. [1] In melanoma cell lines, the inhibitory effect by PLX4032 depends on B-RAF mutational status, because PLX4032 potently inhibits those harboring B-RAF V600 mutants, including V600E, V600D, V600K, and V600R, but not wildtype or other mutants. The IC50 values of PLX4032 on these cells, including MALME-3M, Colo829, Colo38, A375, SK-MEL28, and A2058, ranges from 20 nM to 1 μM. In these cells, PLX4032 (0.1 μM to 30 μM) also inhibits the phosphorylation of both MEK1/2 and ERK1/2. [2] PLX4032 is highly effective in the treatment of melanoma, for its ability of inhibiting B-RAFV600E. However, PLX4032 displays limited effect in colon cancer patients that also carrying B-RAFV600E oncoprotein. The reason for this is that, in colon cancer cells, B-RAFV600E inhibition by PLX4032 results in a rapid feedback EGFR activation, which compensates for the PLX4032-inhibited cell proliferation. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SKMEL19 NGTYdIFHfW6ldHnvckBCe3OjeR?= MmfaOkDPxE1? MV[0PEBp MX7EUXNQ NX7K[5RoXHKrZ3fldpMhTVJic4Ty[ZN{ MmHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NkKyOFAoRjJ|M{[yNlQxRC:jPh?=
VMM12 M2TIdWZ2dmO2aX;uJGF{e2G7 MmnYN{DPxE1? MYe0PEBp MX\EUXNQ MmrjTY5kemWjc3XzJINwdGyjZ3XuJJN6dnSqZYPpd{BidmRiZHXjdoVie2W|IFnMMVkh\XiycnXzd4lwdg>? NF7UcII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm4PVUxPid-MkW5PFk2ODZ:L3G+
C4 MnTiSpVv[3Srb36gRZN{[Xl? MYWzJO69VQ>? M2ewUVQ5KGh? M3jxcWROW09? NX34TJJSUW6lcnXhd4V{KGOxbHzh[4VvKHO7boTo[ZNqeyCjbnSg[IVkemWjc3XzJGlNNThiZYjwdoV{e2mxbh?= NWnmZng6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5PFk2ODZpPkK1PVg6PTB4PD;hQi=>
Calu-6 M1vubGZ2dmO2aX;uJGF{e2G7 MlLsNgKBkc7:TR?= MUOxJIg> MVXEUXNQ MYrBZ5RqfmG2ZYOgUWVMN0WUSzDpckBk\WyuczD3bZRpKHerbHSteJlx\SCEUlHG NXfy[2RERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxO|k4ODVpPkKwNVc6PzB3PD;hQi=>
PC NVi3eJVxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTiPVYhcA>? NGDrVolGSzVyPjCxNFAxKG6P MoHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OEC3PVIoRjF7OEiwO|kzRC:jPh?=
TPC-1 (RET/PTC1) M3y3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHrdIhGQTZiaB?= NYWzbWg{TUN3MPMJqVExODBibl2= NYPKb2NtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PFA4QTJpPkG5PFgxPzl{PD;hQi=>
CAL62 (KRAS G12R) > 1000 > 1000 NY\0NVV5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\NXXo6PiCq M125RWVEPTB-IEGwNFAhdk1? MoW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OEC3PVIoRjF7OEiwO|kzRC:jPh?=
HTH7 (NRAS Q61R) M{DMeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWO5OkBp MYLFR|Ux6onnIEGwNFAhdk1? MkT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OEC3PVIoRjF7OEiwO|kzRC:jPh?=
C643 (HRAS G13R)≥ 500 NWLRN5RUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fIZ|k3KGh? NF\mSpRGSzVyIPMJqUA2ODBibl2= NVWxUJJLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4PFA4QTJpPkG5PFgxPzl{PD;hQi=>
BCPAP (BRAF WT/V600E) Mon2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPvbYs6PiCq MY\FR|UxRTd6IH7N MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh6MEe5Nkc,OTl6OEC3PVI9N2F-
BHT101 (BRAF WT/V600E) MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jZVlk3KGh? NH7iR|NGSzVyPUm3JI5O M1;HOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEiwO|kzLz5zOUi4NFc6OjxxYU6=
SW1736 (BRAF WT/V600E) NUCzRlAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nz[|k3KGh? MnvDSWM2OD1{OTDuUS=> M{\5O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEiwO|kzLz5zOUi4NFc6OjxxYU6=
8505C (BRAF V600E/V600E) MnvzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIn5SIY6PiCq NULRepROTUN3ME21O{BvVQ>? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh6MEe5Nkc,OTl6OEC3PVI9N2F-
ARO NXLabJBlTnWwY4Tpc44hSXO|YYm= NIDKcmEyOCEQvF2= NVrwb4lIPzJiaB?= M1vLRWROW09? NXLXU4I5UW6mdXPld{B1cGVicnXlfJBz\XO|aX;uJI9nKHSqZTDOTXMheHWvcB?= MnTkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NUiwOVMoRjF6NEW4NFU{RC:jPh?=
A375 NHPxd5ZCeG:ydH;zbZMhSXO|YYm= NFLKb5kyOCEQvF2= MXXEUXNQ M2K3O3Bzd22xdHXzJIFxd3C2b4TpZ{Bl\WG2aB?= M3n1SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEW4NFU{Lz5zOES1PFA2OzxxYU6=
TPCI MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIiwNXIyODBizszN NXnsSlN1QTZiaB?= MXXEUXNQ NIjufG1KSzVyPUGwMlc4KM7:TR?= MmDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NUiwOVMoRjF6NEW4NFU{RC:jPh?=
ARO NI\mTYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkCxNVAxKM7:TR?= M13HVFk3KGh? NF7TSnJFVVOR M3vSVGlEPTB;MkC1JI5O MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR3OEC1N{c,OTh2NUiwOVM9N2F-
NPA M1nrSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWSxNFAh|ryP MlL1PVYhcA>? M3OzeWROW09? NIW1foxKSzVyPUK2JI5O M4L0XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEW4NFU{Lz5zOES1PFA2OzxxYU6=
A375 NIW2U4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrORlQyODBizszN M37VU|k3KGh? MmTqSG1UVw>? MofiTWM2OD12NzDuUS=> NUHVZ4NJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0OVgxPTNpPkG4OFU5ODV|PD;hQi=>
UKF-NB-3 (ABCB1) MlKySpVv[3Srb36gRZN{[Xl? NVHScllPOS5{NTFCuW0> MYqyJIg> Mn[xSG1UVw>? M4C3e2VvcGGwY3XzJIFk[3WvdXzheIlwdiCxZjD0bIUh\my3b4Lld4NmdnRiQVLDRlEhe3Wkc4TyZZRmKHKqb3ThcYlv\SBzMkO= NIPtcGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEezOVc3Pid-MkS3N|U4PjZ:L3G+
UKF-NB-3 MX3GeY5kfGmxbjDBd5NigQ>? NILtbYgyNjJ3INM1US=> M4XS[VIhcA>? MlXTSG1UVw>? Mm\sV4lodmmoaXPhcpRtgSCjZn\lZ5R{KG:wIHHjZ5VufWyjdHnvckBw\iC2aHWg[ox2d3Knc3PlcpQhSUKFQkGgd5Vje3S{YYTlJJJpd2SjbXnu[UAyOjN? NV\LTFZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|U4PjZpPkK0O|M2PzZ4PD;hQi=>
A375 (BRAFV600E) NXjxO2lVTnWwY4Tpc44hSXO|YYm= NUHlbId4QCCq M2nLN2ROW09? NUTqcpRTUW6lcnXhd4V{KGmwdILhZ4VtdHWuYYKgVm9UKGGwZDDOU{Bt\X[nbIO= NXS4bWV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzOlM3PDRpPkK1N|Y{PjR2PD;hQi=>
A375P MX3BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NIHFdIRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGzO|VRKGOnbHzzMEBKSzVyIE2gNE4zPTRizszNMi=> M2X2SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NE[wNFMxLz5{MkS2NFA{ODxxYU6=
A375 NIPBRWVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHv3flNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGzO|Uh[2WubIOg[ZhxemW|c3nu[{BDNVKjZjDWOlAxTSCvdYThcpQh[W6mIIfpcIQhfHmyZTDSZZMtKEmFNUCgQUAxNjNzIN88UU4> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhyOEmxNUc,OjJ6MEi5NVE9N2F-
A375P M2W0TWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MWS0PEBpenN? NHTOe4pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGzO|VRKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlI2KM7:TT6= MnLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMki0NVAoRjJ2MUK4OFExRC:jPh?=
A375 NHSyR3pEgXSxdH;4bYNqfHliYYPzZZk> NXrQbJhQPzJiaILz MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlE5KM7:TT6= MnezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUW4NVgoRjJ2MkG1PFE5RC:jPh?=
SK-MEL-28 NYfJe2JUS3m2b4TvfIlkcXS7IHHzd4F6 NUPXPYVkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVI5KGOnbHzzJIV5eHKnc4PpcochSi2{YX[gWlYxOEVibYX0ZY51NCCLQ{WwJF0hOC5zIN88UU4> M336RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEexOFY3Lz5{NES3NVQ3PjxxYU6=
M229 MljQR5l1d3SxeHnjbZR6KGG|c3H5 MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNNlI6KGOnbHzzJIV5eHKnc4PpcochSi2{YX[gWlYxOEVibYX0ZY51NCCLQ{WwJF0hOC5zIN88UU4> NV3KfZplRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|E1PjZpPkK0OFcyPDZ4PD;hQi=>
M263 M2rtWGN6fG:2b4jpZ4l1gSCjc4PhfS=> MojSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUVI3OyClZXzsd{BmgHC{ZYPzbY5oKEJvcnHmJHY3ODCHIH31eIFvfCxiSVO1NEA:KDBwMTFOwG0v M1[3UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEexOFY3Lz5{NES3NVQ3PjxxYU6=
M321 MV3DfZRwfG:6aXPpeJkh[XO|YYm= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNN|IyKGOnbHzzJIV5eHKnc4PpcochSi2{YX[gWlYxOEVibYX0ZY51NCCLQ{WwJF0hOC5zIN88UU4> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MUS2Okc,OjR2N{G0OlY9N2F-
M238 M3LMb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NUPJdXdvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVTJ|ODDj[YxteyCneIDy[ZN{cW6pIFKtdoFnKFZ4MEDFJI12fGGwdDygTWM2OCB;IECuNUDPxE1w Mn:zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{G0OlYoRjJ2NEexOFY3RC:jPh?=
M262 NX3Db2htS3m2b4TvfIlkcXS7IHHzd4F6 MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNNlYzKGOnbHzzJIV5eHKnc4PpcochSi2{YX[gWlYxOEVibYX0ZY51NCCLQ{WwJF0hOC5zIN88UU4> MknNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{G0OlYoRjJ2NEexOFY3RC:jPh?=
M249 M2LSdWN6fG:2b4jpZ4l1gSCjc4PhfS=> NXzyNIlCS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVTJ2OTDj[YxteyCneIDy[ZN{cW6pIFKtdoFnKFZ4MEDFJI12fGGwdDygTWM2OCB;IECuNUDPxE1w NVLJV5F3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|E1PjZpPkK0OFcyPDZ4PD;hQi=>
M14 M2PjPGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlXHR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUVE1KGOnbHzzJIV5eHKnc4PpcochVlKDUzDHNVJEKG23dHHueEwhUUN3MDC9JFAvOTVizszNMi=> M3vhc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEexOFY3Lz5{NES3NVQ3PjxxYU6=
SK-MEL-28 NXX5NZJySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NIT4[3Y3QCCqcoO= M{TKd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1utUWVNNTJ6IHPlcIx{KGijcnLvdolv\yCEUlHGJHY3ODCHIH31eIFvfCCjZoTldkA3QCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hOC52ODFOwG0v MmjlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3OEiwO|MoRjJ2NUi4NFc{RC:jPh?=
insect cell M{TXN2Z2dmO2aX;uJIF{e2G7 NXfzcWhnPjBibXnudy=> MVjJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKEJvUnHmJHY3ODCHIH31eIFvfCCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6|ZXP0JINmdGy|IHHzd4V{e2WmIHHzJHto[W2vYT2zN3BecW6lb4Lwc5JifGmxbjDpcpRwKE2HSzDh[pRmeiB4MDDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUCgQUAxNjB|MTFOwG0v NWnXN45jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA{OTVpPkK0PVAxOzF3PD;hQi=>
MALME-3M MnOySpVv[3Srb36gZZN{[Xl? NXm2[JdNOSCqch?= NX:zPFEzUW6qaXLpeIlwdiCxZjDCMXJi\iCYNkCwSUBufXSjboStcYVlcWG2ZXSgSZJsKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBOSUyPRT2zUUBk\WyuczDh[pRmeiBzIHjyJIJ6KG[udX;y[ZNk\W6lZTDhcoFtgXOrczygTWM2OCB;IECuNFYyKM7:TT6= NWDtWHliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA{OTVpPkK0PVAxOzF3PD;hQi=>
A375 MlfJSpVv[3Srb36gZZN{[Xl? NGHSNIIyKGi{ NYPtbnR1UW6qaXLpeIlwdiCxZjDCMXJi\iCYNkCwSUBufXSjboStcYVlcWG2ZXSgSZJsKHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBCOzd3IHPlcIx{KGGodHXyJFEhcHJiYomg[ox2d3Knc3PlcoNmKGGwYXz5d4l{NCCLQ{WwJF0hOC5zOTFOwG0v NFLmSHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFMyPSd-MkS5NFA{OTV:L3G+
COLO205 M2Gw[GN6fG:2b4jpZ4l1gSCjc4PhfS=> MX20JIRigXN? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDU2xQOjB3IHPlcIx{KGGodHXyJFQh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHHzd4F6NCCHQ{WwJF0hOC5{NDFOwG0v MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEOxOUc,OjR7MECzNVU9N2F-
WM266.4 MVHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NIrj[ow1QCCqcoO= NH31NnZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGfNNlY3NjRiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODZizszNMi=> NIXkVno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK2O|AxPid-MkWyOlcxODZ:L3G+
A375 MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGrQTGw1QCCqcoO= MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF|N{WgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMUmg{txONg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ4N{CwOkc,OjV{NkewNFY9N2F-
WM1361 MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NV;qe29LPDhiaILz MnvBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCZTUGzOlEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuPFch|ryPLh?= M{LKOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Mk[3NFA3Lz5{NUK2O|AxPjxxYU6=
A375 MXvGeY5kfGmxbjDhd5NigQ>? M2H0dWlvcGmkaYTpc44hd2ZiQj3yZYYhXjZyMFWgcZV1[W62IHnuJIh2dWGwIFGzO|Uh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFXST|EwOiCyaH;zdIhwenmuYYTpc44hcW6ldXLheIVlKG[xcjC5NEBucW6|IHL5JHdme3Sncn6gZoxwfHSrbnegcYV1cG:mLDDJR|UxKD1iMD6wN|MyKM7:TT6= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MkK2O{c,OjV2NkKyOlc9N2F-
A375 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mny1O|IhcHK| M2PZRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUO3OUBk\WyuczDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IEOuN|E2KM7:TT6= NIHpNI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NlI3Pyd-MkW0OlIzPjd:L3G+
SK-MEL-2 NUO3VZlXTnWwY4Tpc44h[XO|YYm= MV;Jcohq[mm2aX;uJI9nKHerbHSgeJlx\SCELYLh[kBqdiCqdX3hckBUUy2PRVytNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iRWLLNU8zKHCqb4PwbI9zgWyjdHnvckBqdmO3YnH0[YQh\m:{IEmwJI1qdnNiYomgW4V{fGW{bjDicI91fGmwZzDt[ZRpd2R? NGXQVXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NlI3Pyd-MkW0OlIzPjd:L3G+
A375 NXrmXotVTnWwY4Tpc44h[XO|YYm= NW\YXIU6PzJiaILz NISxVG9KdmirYnn0bY9vKG:oIFLSRWYhXjZyMFWgcZV1[W62IHnuJIh2dWGwIFGzO|Uh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDFVmsheGixc4Doc5J6dGG2aX;uJI1m[XO3cnXkJIFnfGW{IEeyJIhzeyCkeTDFUGlUSSCjc4PhfUwhUUN3MDC9JFAvOTVizszNMi=> NWXNbJRYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OlU5ODRpPkK1PVY2QDB2PD;hQi=>
A375 MnPyRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NH;FT4o4OiCqcoO= MnrCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDM{e1JINmdGy|IHHmeIVzKDd{IHjyd{BjgSC{ZYPhfpVzcW5iYYPzZZktKEmFNUCgQUAxNjF5IN88UU4> M4[xeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OU[1PFA1Lz5{NUm2OVgxPDxxYU6=
A375 NULvfWZQTnWwY4Tpc44h[XO|YYm= M3H2R|E2KG2rboO= NGrNU3FEd22yZYTpeIl3\SCkaX7kbY5oKGGoZnnubZR6KHSxIFLSRWYhcW5iaIXtZY4hSTN5NTDj[YxteyCjZoTldkAyPSCvaX7zJIlvKHC{ZYPlcoNmKG:oIFHUVEBidmGub3f1[UwhUUN3MDC9JFAvOjZizszNMi=> Mk\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkW4NFQoRjJ3OU[1PFA1RC:jPh?=
A375 MljvSpVv[3Srb36gZZN{[Xl? Mk\1NVUhdWmwcx?= Mli5R49ueGW2aYTpeoUh[mmwZHnu[{Bi\m[rbnn0fUB1dyCDUlHGJIlvKGi3bXHuJGE{PzViY3XscJMh[W[2ZYKgNVUhdWmwczDpckBxemW|ZX7j[UBw\iCDVGCgZY5idG:pdXWsJGlEPTBiPTCwMlk2KM7:TT6= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl4NUiwOEc,OjV7NkW4NFQ9N2F-
HCT116 MlL2SpVv[3Srb36gZZN{[Xl? Mn\zTY5pcWKrdHnvckBw\iCNUlHTJGcyO0RibYX0ZY51KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFXST{BxcG:|cHjvdplt[XSrb36gZpkhTUyLU1GsJGlEPTBiPTCxOk43KM7:TT6= Ml:3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkW4NFQoRjJ3OU[1PFA1RC:jPh?=
HCT116 NHvVcmlHfW6ldHnvckBie3OjeR?= MlTTNE4{PCC2bzCyNFAxOCCwTR?= NF3DR5lR[XKjZH;4bYNidCCjY4TpeoF1cW:wIH;mJHJCWy:UQV[vUWVMKHOrZ37hcIlv\yCyYYToe4F6KGmwIHj1cYFvKEiFVEGxOkBk\WyuczDlfJBz\XO|aX7nJJdqdGRidInw[UBDWkGIIHHzd4V{e2WmIHHzJGVTUyCyaH;zdIhwenmuYYTpc44h[XRiMD6zOEB1dyB{MECwNEBvVQ>? Ml3SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NkW4NFQoRjJ3OU[1PFA1RC:jPh?=
NZM20 M3rmdGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NF\BZpQ3QCCqcoO= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6cTUKwJINmdGy|IHX4dJJme3OrbnegRk1T[WZiVk[wNGUhdXW2YX70JIl{d2yjdHXkJIZzd21iTnX3JHpm[WyjbnSgcYV1[XO2YYTpZ{Bu\Wyjbn;tZUBx[XSrZX70JIlv[3WkYYTl[EBnd3JiNkigbJJ{KGK7IGPSRkBie3OjeTygTWM2OCB;IECuNFI1KM7:TT6= M3u5NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEC1OVMxLz5{NkCwOVU{ODxxYU6=
NZM07 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M1GzPVY5KGi{cx?= NVPDTHA3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOXm0xPyClZXzsd{BmgHC{ZYPzbY5oKEJvUnHmJHY3ODCHIH31eIFvfCCrc3;sZZRm\CCocn;tJG5mfyCcZXHsZY5lKG2ndHHzeIF1cWNibXXsZY5wdWFicHH0bYVvfCCrbnP1ZoF1\WRiZn;yJFY5KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjB|NjFOwG0v MnflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMEW1N|AoRjJ4MEC1OVMxRC:jPh?=
A375 MXzBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NVT1O|ZoPjhiaILz NYfjSHFxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBN|c2KGOnbHzzJIV5eHKnc4PpcochSi2UYX[gWlYxOEVibYX0ZY51KGmwY4XiZZRm\CCob4KgOlghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODd7IN88UU4> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByNUWzNEc,OjZyMEW1N|A9N2F-
COLO205 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXL4cJR6PjhiaILz MkDzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCFT1zPNlA2KGOnbHzzJIV5eHKnc4PpcochSi2UYX[gWlYxOEVibYX0ZY51KGmwY4XiZZRm\CCob4KgOlghcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOzB7IN88UU4> NWTaNpR{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFU2OzBpPkK2NFA2PTNyPD;hQi=>
SK-MEL-28 M2fx[GFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3T5XlY5KGi{cx?= NH\kOnlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW1GVC1{ODDj[YxteyCneIDy[ZN{cW6pIFKtVoFnKFZ4MEDFJI12fGGwdDDpcoN2[mG2ZXSg[o9zKDZ6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkO4NUDPxE1w NXW5T4xKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFU2OzBpPkK2NFA2PTNyPD;hQi=>
HT-29 NWfjcWlFSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Ml:2OlghcHK| MofHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVD2yPUBk\WyuczDlfJBz\XO|aX7nJGIuWmGoIG[2NFBGKG23dHHueEBqdmO3YnH0[YQh\m:{IE[4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlYxOSEQvF2u MnjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMEW1N|AoRjJ4MEC1OVMxRC:jPh?=
SK-MEL-1 MVnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MoDKOlghcHK| NH[w[XNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW1GVC1zIHPlcIx{KGW6cILld5NqdmdiQj3SZYYhXjZyMFWgcZV1[W62IHnuZ5Vj[XSnZDDmc5IhPjhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDFwNEm5JO69VS5? NFrlcHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCwOVU{OCd-Mk[wNFU2OzB:L3G+
NZM40 NWXLU2tHSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MkS2OlghcHK| MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6cTUSwJINmdGy|IHX4dJJme3Orbnege4lt\CC2eYDlJGIuWmGoIHnzc4xifGWmIH\yc40hVmW5IGrlZYxidmRibXX0ZZN1[XSrYzDt[Yxidm:vYTDwZZRq\W62IHnuZ5Vj[XSnZDDmc5IhPjhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDNwMEGg{txONg>? NVS1eG1URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wNFU2OzBpPkK2NFA2PTNyPD;hQi=>
NZM09 MXLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NITQRoU3QCCqcoO= NYDrdWJ2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOXm0xQSClZXzsd{BmgHC{ZYPzbY5oKHerbHSgeJlx\SCELWLh[kBqe2:uYYTl[EBnem:vIF7le{Bb\WGuYX7kJI1mfGG|dHH0bYMhdWWuYX7vcYEheGG2aXXueEBqdmO3YnH0[YQh\m:{IE[4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTBiPTC4MlM{KM7:TT6= M1;hNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEC1OVMxLz5{NkCwOVU{ODxxYU6=
A375P NXvVdlBoSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mo\jO|IhcHK| M3XnXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUO3OXAh[2WubIOg[ZhxemW|c3nu[{BDWkGIIG[2NFBGKG23dHHueEBi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyMWdtdyCjc4PhfUwhUUN3MDC9JFAvOzdizszNMi=> NFO0cHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkeyOFc{OCd-Mk[3NlQ4OzB:L3G+
BL21(DE3) MoTnSpVv[3Srb36gZZN{[Xl? NXvRTZJEUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJIhqey22YXfn[YQhSlKDRjDWOlAxTSCvdYThcpQhMDR2ODD0c{A4OjNicnXzbYR2\XNrIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSiGRUOpJINmdGy|IHHzd4V{e2WmIHHzJJBpd3OyaH;yfYxifGmxbjDv[kBjcW:2aX75cIF1\WRvTVXLJIJ6KEGucHjhV4Nz\WWwIHHzd4F6NCCLQ{WwJF0hOC5yM{Gg{txONg>? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh3Mk[yN{c,OjZ6NUK2NlM9N2F-
A375 MXTGeY5kfGmxbjDhd5NigQ>? MWGxJIhz NXnPcmZUUW6qaXLpeIlwdiCxZjDCMXJi\iCYNkCwSUBufXSjboSgbY4hcHWvYX6gRVM4PSClZXzsd{Bie3Onc4Pl[EBieyCHUlugdIhwe3Cqb4L5cIF1cW:wIIDy[Ylv[3WkYYTl[EBnd3JiMTDodkBjgSCZZYP0[ZJvKGKub4SgcYV1cG:mLDDJR|UxKD1iMD6wNVch|ryPLh?= NGKyZWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C4OVY4Oid-MkewPFU3PzJ:L3G+
MIAPaCa2 MWnGeY5kfGmxbjDhd5NigQ>? Mn24NUBpeg>? MVrJcohq[mm2aX;uJI9nKHerbHSgeJlx\SCELWLh[kBqdiCqdX3hckBOUUGSYVPhNkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iRWLLJJBpd3OyaH;yfYxifGmxbjDwdoVqdmO3YnH0[YQh\m:{IEGgbJIh[nliV3XzeIVzdiCkbH;0JI1mfGixZDygSWM2OCB;IEKuNlkh|ryPLh?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB6NU[3Nkc,OjdyOEW2O|I9N2F-
COLO205 NX7KR5RRS3m2b4TvfIlkcXS7IHHzd4F6 M2nxR|czKGi{cx?= MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDU2xQOjB3IHPlcIx{KGijcnLvdolv\yCELWLh[kBXPjByRTDteZRidnRiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNFQ1KM7:TT6= NV7BZ4g4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOVU5QTlpPkK3NVU2QDl7PD;hQi=>
HT-29 NFfhZmREgXSxdH;4bYNqfHliYYPzZZk> Mn7zO|IhcHK| NH21RVBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJXC1{OTDj[YxteyCqYYLic5JqdmdiQj3SZYYhXjZyMFWgcZV1[W62IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlE2PiEQvF2u MmfiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzNUW4PVkoRjJ5MUW1PFk6RC:jPh?=
HCT116 NYTVTVh2S3m2b4TvfIlkcXS7IHHzd4F6 NULZ[mFTPzJiaILz M3XSNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhEXDFzNjDj[YxteyCqYYLic5Jqdmdid3ns[EB1gXCnIFKtVoFnKGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzND61PEDPxE1w NX20U4FXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexOVU5QTlpPkK3NVU2QDl7PD;hQi=>
WM266.4 NInXc3dCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NV31NoNbOjRiaILz M13YSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iV12yOlYvPCClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBIUTVyIE2gNE4zOSEQvF2u MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzJ|OEi0NUc,Ojd{M{i4OFE9N2F-
WM266.4 MkP6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NEi3fGUzPCCqcoO= Mn:5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCZTUK2Ok41KGOnbHzzJIhiemKxcnnu[{BDWkGIIG[2NFBGKG23dHHueEBie3Onc4Pl[EBieyClZXzsJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlA4KM7:TT6= NVzWSVltRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2N|QyQTVpPkK3OlM1OTl3PD;hQi=>
A375 NWO3UoVySW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NXjx[pdSOjRiaILz NHXjWJZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFGzO|Uh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB{NDDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD6yNUDPxE1w NVnMcpE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2N|QyQTVpPkK3OlM1OTl3PD;hQi=>
WM1361 MXPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{\N[VI1KGi{cx?= NYjUN2pqSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDXUVE{PjFiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEBoem:5dHigbY5pcWKrdHnvckBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNU45PiEQvF2u Moi4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4M{SxPVUoRjJ5NkO0NVk2RC:jPh?=
SK-MEL-28 Mnq2RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NG\IUYM1QCCqcoO= M13QTWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1utUWVNNTJ6IHPlcIx{KGijcnLvdolv\yCEUlHGJHY3ODCHIH31eIFvfCCjc4Pld5Nm\CCjczDjc45k\W62cnH0bY9vKHKncYXpdoVlKG[xcjD0c5RidCCpcn;3eIghcW6qaXLpeIlwdiCvZXHzeZJm\CCjZoTldkA1QCCqcoOgdoV{[Xq3cnnuJIF{e2G7LDDUS2khRSB{IN88UU4> MnS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{SxN|coRjJ5N{e0NVM4RC:jPh?=
SK-MEL-28 M1;0dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV2xJIhz NVzUb5llT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVI5KGOnbHzzJIhiemKxcnnu[{BDWkGIIG[2NFBGKG23dHHueEBxemWrbnP1ZoF1\WRiZn;yJFEhcHJiZn;scI94\WRiYomgbZJz[WSrYYTpc44hd2ZiMT6xN{BsXy:vMjDVWk1tcWeqdDDmc5IhPSCvaX7zJI1m[XO3cnXkJIFnfGW{IES4JIhzeyC{ZYPhfpVzcW5iYYPzZZk> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd5NEGzO{c,Ojd5N{SxN|c9N2F-
A375 MkXiRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUO3NkBpenN? MlvrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbJVu[W5iQUO3OUBk\WyuczDh[pRmeiB5MjDodpMh[nliY3XscEB1cXSncj3ncI8hdHWvaX7ld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC55IN88UU4> M3;YVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSyOVU{Lz5{OEK0NlU2OzxxYU6=
COLO205 M{GxfmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NEHLRZQ4OiCqcoO= NWLhUHY6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkhcHWvYX6gR29NVzJyNTDj[YxteyCjZoTldkA4OiCqcoOgZpkh[2WubDD0bZRmei2pbH:gcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyIE2gOU4yPiEQvF2u MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh{NEK1OVMoRjJ6MkSyOVU{RC:jPh?=
HepG2 MnfvRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mn7IO|IhcHK| NF6ySWxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC3NkBpenNiYomgZ4VtdCC2aYTldk1odG9ibIXtbY5me2OnbnPlJIF{e2G7LDDJR|UxKD1iNT60PEDPxE1w NUKxfo9uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyOFI2PTNpPkK4NlQzPTV|PD;hQi=>
SK-MEL-2 M3v4emFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnTFO|IhcHK| MnnYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbJVu[W5iU1utUWVNNTJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JINmdGxidHn0[ZIu\2yxIHz1cYlv\XOlZX7j[UBie3OjeTygTWM2OCB;IEWuOlQh|ryPLh?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ2MkW1N{c,Ojh{NEK1OVM9N2F-
K562 MW\GeY5kfGmxbjDhd5NigQ>? MkfuNUBpeg>? MoHLV5Ri[mmuaYrheIlwdiCxZjDCVmFHKGmwIHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhOSCqcjDifUB1cGW{bXHsJJNpcW[2IHHzd4F6NCCHQ{WwJF0hOC55OUSzN{DPxE1w MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ6MEK2NUc,Ojh{OECyOlE9N2F-
K562 NGDyOG1HfW6ldHnvckBie3OjeR?= MVuxJIhz MnXkV5Ri[mmuaYrheIlwdiCxZjDGSWNJKGmwIHj1cYFvKEt3NkKgZ4VtdHNiYX\0[ZIhOSCqcjDifUB1cGW{bXHsJJNpcW[2IHHzd4F6NCCHQ{WwJF0hPS5yMUG4O{DPxE1w NFPMSlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK4NFI3OSd-MkiyPFAzPjF:L3G+
A375M Ml3JRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2KxdVczKGi{cx?= MXjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF|N{XNJINmdGy|IHjhdoJwemmwZzDCVmFHKFZ4MEDFJI12fGGwdDDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMD61JO69VS5? NEG1UW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES1PFE{PCd-Mki0OVgyOzR:L3G+
1205 Lu Mmf0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NYnzWnkyPzJiaILz NWrzRmpoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjCxNlA2KEy3IHPlcIx{KGijcnLvdolv\yCEUlHGJHY3ODCHIH31eIFvfCCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOiEQvF2u Mm\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUixN|QoRjJ6NEW4NVM1RC:jPh?=
A375M MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MkixOFghcHK| MmKwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDM{e1UUBk\WyuczDoZZJjd3KrbnegRnJCTiCYNkCwSUBufXSjboSgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKuNFUh|ryPLh?= M3LVOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEW4NVM1Lz5{OES1PFE{PDxxYU6=
UACC-903 NXvqOo1pSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MnzzO|IhcHK| M2nrSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVVHDR{06ODNiY3XscJMhcGG{Yn;ybY5oKEKUQV[gWlYxOEVibYX0ZY51KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjdizszNMi=> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3OEGzOEc,Ojh2NUixN|Q9N2F-
1205 Lu NHS0TnNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NUD2S445PDhiaILz MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKDF{MEWgUJUh[2WubIOgbIFz[m:{aX7nJGJTSUZiVk[wNGUhdXW2YX70JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC3MlYh|ryPLh?= Mn7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2NUixN|QoRjJ6NEW4NVM1RC:jPh?=
UACC-903 NYG3[Wx6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NV3ERYFxPDhiaILz M4rlSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVVHDR{06ODNiY3XscJMhcGG{Yn;ybY5oKEKUQV[gWlYxOEVibYX0ZY51KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyOi5|IN88UU4> M{HoUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEW4NVM1Lz5{OES1PFE{PDxxYU6=
CHL-1 M{nLd2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGDMTZM4OiCqcoO= M3rhV2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1jMMVEh[2WubIOgbIFz[m:{aX7nJJdqdGRidInw[UBDWkGIIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzMj63JO69VS5? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR3OEGzOEc,Ojh2NUixN|Q9N2F-
CHL-1 MV7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{m4XFQ5KGi{cx?= NWS1foNRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDTGwuOSClZXzsd{Bp[XKkb4Lpcochf2muZDD0fZBmKEKUQV[gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEKwJO69VS5? NH3iU2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES1PFE{PCd-Mki0OVgyOzR:L3G+
HCT116 Mnv0SpVv[3Srb36gZZN{[Xl? NX;EdVJqW3SrbYXsZZRqd25ib3[gRnJCTi2FUlHGJIRqdWW{aYrheIlwdiCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZpkhdHWlaX\ldoF{\SClb33wcIVu\W62YYTpc44h[XO|YYmsJGVEPTBiPTCwMlYxOSEQvF2u NYnH[IJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1OVc1PThpPkK4OVU4PDV6PD;hQi=>
Calu6 MXzGeY5kfGmxbjDhd5NigQ>? MoT6N{B2VQ>? NXrZOGxjOiCqcoO= NVTueXBnSWO2aY\heIlwdiCxZjDDVmFHKGmwIHj1cYFvKEOjbIW2JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKE2HSzDwbI9{eGixconsZZRqd25iYYSgN{B2VSCjZoTldkAzKGi{czDifUBHWkWWIHHzd4F6 MkfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3NUe0OVgoRjJ6NUW3OFU5RC:jPh?=
Sf9 MUnGeY5kfGmxbjDhd5NigQ>? NVXJUJdsOSCqch?= MUXJcohq[mm2aX;uJI9nKGi3bXHuJHpCUyBqNTD0c{A{ODlicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCVZkmgbY5{\WO2IHPlcIx{KHW|aX7nJHpCU3SrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFEhcHJiYomgcYF{eyC|cHXjeJJwdWW2comsJGlEPTBiPTCwMlAzOyEQvF2u NXPXPGdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1PFYzOTFpPkK4OVg3OjFzPD;hQi=>
Sf9 MnvjSpVv[3Srb36gZZN{[Xl? M3LpfFMxKG2rboO= NF;oZpJKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBz\WOxbXLpcoFvfCCqdX3hckBnfWyuLXzlcod1cCCcQVug[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIGPmPUBqdnOnY4SgZ4VtdHNidYPpcochVUKSIHHzJJN2[nO2cnH0[UBi\nSncjCzNEBucW6|IHL5JGFFWC2JbH:gZZN{[XluIFnDOVAhRSByLkCzNVQh|ryPLh?= NGnFfXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW4OlIyOSd-Mki1PFYzOTF:L3G+
UACC-903 MnXSR5l1d3SxeHnjbZR6KGG|c3H5 NFz1OGczPSC3TR?= MoTOOFghcHK| Mm[1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWWFESy17MEOgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYYSgNlUhfU1iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSC{ZXzheIl3\SC2bzDjc451em:uLDDJR|UxKD1iMz62JO69VS5? M13HZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MUOzNFM2Lz5{OUGzN|A{PTxxYU6=
CHL-1 NH3pcXhCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NVv5cm4xPzJiaILz NVS0XnlESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDTGwuOSClZXzsd{Bp[XKkb4Lpcochf2muZDD0fZBmKEKUQV[gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGzMlch|ryPLh?= NW[5NZViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxN|MxOzVpPkK5NVM{ODN3PD;hQi=>
A375 NGDrVIFHfW6ldHnvckBie3OjeR?= MlLoPVYhcHK| MknaTY5pcWKrdHnvckBw\iCEUlHGJHY3ODCHIH31eIFvfCCrbjDoeY1idiCDM{e1JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJBzd2yrZnXyZZRqd25iaX7jeYJifGWmIH\vdkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5zMkeg{txONg>? NXfpRZhNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NFc6PzdpPkK5OFA4QTd5PD;hQi=>
A375 NXTZfZlTTnWwY4Tpc44h[XO|YYm= NE\QVHJKdmirYnn0bY9vKG:oIFLSRWYhXjZyMFWgcZV1[W62IHnuJIh2dWGwIFGzO|Uh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFXST{BxcG:|cHjvdplt[XSrb36gZpkhSWyyaHHTZ5Jm\W5iYYPzZZktKEmFNUCgQUAxNjB|MjFOwG0v Mlj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NkG4NlcoRjJ7NE[xPFI4RC:jPh?=
SK-MEL-32 M1nTV2N6fG:2b4jpZ4l1gSCjc4PhfS=> NYPTbnBLS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0tvTVXMMVMzKGOnbHzzMEBKSzVyIE2gNE4{OSEQvF2u M3zQWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[xPFI4Lz5{OUS2NVgzPzxxYU6=
WM266.4 NEHiNmNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3HiN|Q5KGi{cx?= NIXNSIhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGfNNlY3NjRiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhT0l3MDC9JFAvOjFizszNMi=> NXvLTWRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5OFA1PjNpPkK5PVQxPDZ|PD;hQi=>
A375 NXvzVFJPSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NUXEOnpIPDhiaILz MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF|N{WgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDBwOUWg{txONg>? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl2MES2N{c,Ojl7NEC0OlM9N2F-
HT-29 MU\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVq0PEBpenN? NIjUTHlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjUMVI6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGdKPTBiPTCxMlg5KM7:TT6= M1n2PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7OUSwOFY{Lz5{OUm0NFQ3OzxxYU6=
WM1361 NUTEblhiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= Mo[4OFghcHK| NXzxWoNXSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDXUVE{PjFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhT0l3MDC9JFIxNjhizszNMi=> MmPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl7NEC0OlMoRjJ7OUSwOFY{RC:jPh?=
HCT116 M1:1UmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MVe0PEBpenN? MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDHTVUxKD1iMkWuNkDPxE1w NWHtOGhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm5OFA1PjNpPkK5PVQxPDZ|PD;hQi=>
A673 NE\WSppyUFSVIHHzd4F6 MoS4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? NEXVT2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY MoDodWhVWyCjc4PhfS=> NUHWRmtieUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFTBU3kh[2WubIO= NF3PWo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD NUnUZYRUeUiWUzDhd5NigQ>? MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> NXK4NmVqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH MVvxTHRUKGG|c3H5 M2T3[ZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) MVnxTHRUKGG|c3H5 MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? NG\0[FE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 MoDYdWhVWyCjc4PhfS=> MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| MnvqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 M1PTZpFJXFNiYYPzZZk> MmG1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= M2[0fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 M4fZfZFJXFNiYYPzZZk> NUfZNVBneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSTZ5MzDj[Yxteyl? Mk\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS MkHBdWhVWyCjc4PhfS=> MUPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDVMVIhV1NiY3XscJM> NYe1U5h[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 Mkj2dWhVWyCjc4PhfS=> M{i3WJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJM> MnzxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 M4eySJFJXFNiYYPzZZk> NIfqXnlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> NV;vR4V7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MV7xTHRUKGG|c3H5 MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NHPCeXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 NYrTWWpDeUiWUzDhd5NigQ>? M{j4UJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MWDxTHRUKGG|c3H5 Mn[zdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>? NXuzXW1{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 NGfQ[VRyUFSVIHHzd4F6 M2rBd5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NFrUSmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Assay
Methods Test Index PMID
Western blot p-ERK / p-CRAF ; p-MEK(S217/221) / pAKT(T308) / p-AKT(S473) / p-P70 S6K(T389) / p-S6(Ser235-236) / P-4EB-P1 ; Bax / Bcl2 / Bcl-xl / BIM / Mcl1 22448344 22194965 28382170
Growth inhibition assay Cell viability 29179510
Immunofluorescence uPAR / α5-β1 ; p-Akt(Thr308) 30611716 27293997
In vivo In B-RAFV600E-mutant mice xenograft models, PLX4032 (6 mg/kg–20 mg/kg) inhibits tumor growth. [1] In mice xenograft models of LOX, Colo829, and A375 cells, PLX4032 (12.5 mg/kg–100 mg/kg) inhibits tumor growth and prolongs mice survival. [2]

Protocol (from reference)

Kinase Assay:

[1]

  • RAF kinase activity measurements:

    The kinase activities of wild-type RAF and mutants are determined by measuring phosphorylation of biotinylated-BAD protein. For each enzyme (0.01 ng), 20 μL reactions are carried out in 20 mM Hepes (pH 7.0), 10 mM MgCl2, 1 mM DTT, 0.01% (v/v) Tween-20, 50 nM biotin-BAD protein, and 1 mM ATP at room temperature. Reactions are stopped at 5 min with 5 μL of a solution containing 20 mM Hepes (pH 7.0), 200 mM NaCl, 80 mM EDTA, 0.3% (w/v) bovine serum albumin (BSA). The stop solution also includes phospho-BAD (Ser112) antibody, streptavidin-coated donor beads, and protein A acceptor beads. The antibody and beads are pre-incubated in stop solution in the dark at room temperature for 30 min. The final dilution of antibody is 1/2000 and the final concentration of each bead is 10 μg/mL. The assay plates are incubated at room temperature for one hour and then are read on a PerkinElmer AlphaQuest reader. Mutant activities are the average of two different batches of purified protein assayed in duplicate in three different experiments.

Cell Research:

[2]

  • Cell lines: MALME-3M, Colo829, Colo38, A375, SK-MEL28, and A2058 cells
  • Concentrations: 0–10 μM , dissolved in DMSO
  • Incubation Time: 5 days
  • Method:

    Cellular proliferation is evaluated by MTT assay. Briefly, cells are plated in 96-well microtiter plates at a density of 1000 to 5000 cells per well in a volume of 180 μL. PLX4032 is prepared at 10 times the final assay concentration in media containing 1% DMSO. Twenty-four hours after cell plating, 20 μL of the appropriate dilution of PLX4032 are added to plates in duplicate. The plates are assayed for proliferation 6 days after the cells are plated. Percent inhibition is calculated and the IC50 is determined from the regression of a plot of the logarithm of the concentration versus percent inhibition.

Animal Research:

[2]

  • Animal Models: Mice (athymic nude) xenograft models of LOX, Colo829, and A375 cells
  • Dosages: 12.5 mg/kg–100 mg/kg
  • Administration: Oral gavage twice daily

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+5% Tween 80+61% ddH2O
For best results, use promptly after mixing.

1.25mg/mL

Chemical Information

Molecular Weight 489.92
Formula

C23H18ClF2N3O3S

CAS No. 918504-65-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCS(=O)(=O)NC1=C(C(=C(C=C1)F)C(=O)C2=CNC3=C2C=C(C=N3)C4=CC=C(C=C4)Cl)F

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05068752 Recruiting Drug: Vemurafenib|Drug: Sorafenib Pancreas Cancer HonorHealth Research Institute|Bayer|Genentech Inc. October 28 2021 Phase 2
NCT03410875 Active not recruiting Drug: Vemurafenib|Drug: Obinutuzumab Hairy Cell Leukemia|Leukemia|Leukemia Hairy Cell Memorial Sloan Kettering Cancer Center|Dana-Farber Cancer Institute|Yale University February 9 2018 Phase 2
NCT03013491 Active not recruiting Drug: CX-072|Drug: ipilimumab|Drug: vemurafenib Solid Tumor|Lymphoma CytomX Therapeutics January 2017 Phase 1|Phase 2
NCT02441465 Completed Drug: Vemurafenib|Drug: 14C-Labeled Vemurafenib Malignant Melanoma Cancer Hoffmann-La Roche August 13 2015 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.

Frequently Asked Questions

Question 1:
How about the half-life of Vemurafenib(S1267)?

Answer:
It was reported that the half-life of the compound is 57 hours.

Question 2:
The vemurafenib power, when prepared in 4% DMSO/30% PEG 300/5% Tween 80/ddH2O solutions, form a pellet down the tube?

Answer:
When prepare this kind of vehicle, please dissolve the drug in DMSO clearly first. If it dissolves not readily, please sonicate and warm in the water bath at about 45 degree. Then add PEG and Tween. After they mixed homogeneously, then dilute with water.

Tags: buy Vemurafenib (PLX4032) | Vemurafenib (PLX4032) supplier | purchase Vemurafenib (PLX4032) | Vemurafenib (PLX4032) cost | Vemurafenib (PLX4032) manufacturer | order Vemurafenib (PLX4032) | Vemurafenib (PLX4032) distributor