Catalog No.S1949 Synonyms: Vitamin K3

Menadione Chemical Structure

Molecular Weight(MW): 172.18

Menadione(Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ), used as a nutritional supplement.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Choose Selective phosphatase Inhibitors

Biological Activity

Description Menadione(Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ), used as a nutritional supplement.
Cdc25 phosphatase [6] DNA polymerase γ [7]
In vitro

Menadione prevents mitochondrial Ca(2+) uptake in normal pancreatic acinar cells, which permits rapid spread of Ca(2+) throughout the cell. Menadione-induced mitochondrial depolarisation is due to induction of the permeability transition pore. [1] Menadione (nontoxic concentration) results in a brief activation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in the RALA255-10G rat hepatocyte cell line. [2] Menadione is equipotent against multidrug-resistant and parental leukemia cell lines with IC50 values of 13.5 mM and 18 mM, respectively. Menadione inhibits the incorporation of radioactive thymidine, uridine and amino acids into DNA, RNA and protein, respectively, in three humancancer cell lines. [3] Menadione at 1-20 mM dose- and time-dependently inhibits cell proliferation of AR4-2J cells. Menadione (100 mM) causes rapid cell death. Menadione (100 mM) induces DNA smear in electrophoresis indicative of necrosis, while lower concentrations (10-20 mM) induces a DNA ladder indicative of apoptosis. Menadione at 1-20 mM induces wild-type P53, whereas the 100 mM menadione has a minor effect on wild-type P53. [4] Menadione treatment clearly affects the mitochondrial function of Jurkat T cells by inducing a collapse of the inner transmembrane potential (DeltaPsi(m)) and a decrease in inner membrane mass, which could be completely reversed by N-acetylcysteine. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT22 cells MUDGeY5kfGmxbjDhd5NigQ>? M{n0dm5mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBud3W|ZTDIWFIzKGOnbHzzJIF{e2W|c3XkJIF{KHC{ZY\lcpRqd25iZoLvcUBodHW2YX3heIUucW6mdXPl[EBwgGmmYYTpeoUh[2WubDDk[YF1cCCkeTDNWHMh[XO|YYmsJHBFPTB;MD63PVch|ryP NGq4PWgzOzN{N{S2PC=>
HeLa cell NIHRdVZEgXSxdH;4bYNqfHliYYPzZZk> M{TLWGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEinTHGgZ4VtdCCuaX7ld{whTUN3ME2zMlch|ryP MlPONVU{Pjl2MEC=
PaCa2 cell MWPQdo9tcW[ncnH0bY9vKGG|c3H5 Mn\nRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCyYX7jdoVifGmlIHPhdoNqdm:vYTDNbYEhWGGFYUKgZ4VtdCCuaX7lJIJ6KGOxbH;ybY1mfHKrYzDhd5NigSxiSVO1NF03NjJizszN NVfEOnh[OTZ{MU[1NFA>
HT-29 cells M1W4T2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mly4O|IhcA>? MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVkvPjlizszN Mom2NlM4QTF|Nke=
DU145 cells NYrJXZR[S3m2b4TvfIlkcXS7IHHzd4F6 NV;Kb|lMPzJiaB?= NVPHRWx{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hTFVzNEWgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD17Lki2JO69VQ>? MVGyN|c6OTN4Nx?=
MDA-MB-231 cells NVTXW5N5S3m2b4TvfIlkcXS7IHHzd4F6 M4D5RlczKGh? MoO1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUGyMlY5KM7:TR?= M1\uNFI{PzlzM{[3
human A2058 cells NILpSoZEgXSxdH;4bYNqfHliYYPzZZk> NG[1WWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCOjB3ODDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKG[xcn3hfoFvKG[xcn3heIlwdiCjdDDkZZkhPSCkeTDt[YF{fXKrbnegZYJ{d3KkYX7j[UBifCB2M{Cgco0tKEmFNUC9NVMvPCEQvF2= NYjuO|dxOTl7Nkm0NFA>
SAS cells NV3UWo54S3m2b4TvfIlkcXS7IHHzd4F6 MV:0PEBp MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTRXMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVQh|ryP MXKyOFkyOzdzMh?=
HaCaT cells MmD1SpVv[3Srb36gZZN{[Xl? NXTKdXV5PDhiaB?= MXfBcpRqcHmyZYLwdo9tcW[ncnH0bZZmKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIFE[VRiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCjZoTldkA1QCCqcoOgZpkheGijc3WgZ49vfHKjc4SgcYlkem:|Y3;wfUwhUUN3ME2xOU45KM7:TR?= NGS3XWszPDl4NEK0Oi=>
HeLa cells M4jpRmN6fG:2b4jpZ4l1gSCjc4PhfS=> NGPwW2U1QCCq MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE5KM7:TR?= NX3ZUHI2OjR7MUO3NVI>
CRL2796 cells MnXmR5l1d3SxeHnjbZR6KGG|c3H5 MkjXR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR3JNOjd7NjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKG[xcn3hfoFvKG[xcn3heIlwdiCjdDDkZZkhPCCkeTDt[YF{fXKrbnegZYJ{d3KkYX7j[UBifCB2M{Cgco0tKEmFNUC9NlEh|ryP NGnRcXMyQTl4OUSwNC=>
HL60 cells NF;ucG5HfW6ldHnvckBie3OjeR?= M{W1SVUxKM7:TR?= NGLQT|hKdmS3Y4Tpc44hd2ZibnXjdo9{cXNiaX6gbY51[WO2IHj1cYFvKEiONkCgZ4VtdHNiYYPz[ZN{\WRiYYOgZ5l1d3OxbHnjJGxFUCC{ZXzlZZNmKGG2IEWwJJVO NFXCfnYzPTR4MkK3Ny=>

... Click to View More Cell Line Experimental Data


Solubility (25°C)

In vitro DMSO 34 mg/mL (197.46 mM)
Ethanol 34 mg/mL (197.46 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 172.18


CAS No. 58-27-5
Storage powder
in solvent
Synonyms Vitamin K3

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
Tags: buy Menadione | Menadione ic50 | Menadione price | Menadione cost | Menadione solubility dmso | Menadione purchase | Menadione manufacturer | Menadione research buy | Menadione order | Menadione mouse | Menadione chemical structure | Menadione mw | Menadione molecular weight | Menadione datasheet | Menadione supplier | Menadione in vitro | Menadione cell line | Menadione concentration | Menadione nmr
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID