For research use only.

Catalog No.S1949 Synonyms: Vitamin K3

3 publications

Menadione Chemical Structure

Molecular Weight(MW): 172.18

Menadione(Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ), used as a nutritional supplement.

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10mM (1mL in DMSO) USD 130 In stock
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Biological Activity

Description Menadione(Vitamin K3), a fat-soluble compound, is an inhibitor of Cdc25 phosphatase and mitochondrial DNA polymerase γ (pol γ), used as a nutritional supplement.
Cdc25 phosphatase [6] DNA polymerase γ [7]
In vitro

Menadione prevents mitochondrial Ca(2+) uptake in normal pancreatic acinar cells, which permits rapid spread of Ca(2+) throughout the cell. Menadione-induced mitochondrial depolarisation is due to induction of the permeability transition pore. [1] Menadione (nontoxic concentration) results in a brief activation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) in the RALA255-10G rat hepatocyte cell line. [2] Menadione is equipotent against multidrug-resistant and parental leukemia cell lines with IC50 values of 13.5 mM and 18 mM, respectively. Menadione inhibits the incorporation of radioactive thymidine, uridine and amino acids into DNA, RNA and protein, respectively, in three humancancer cell lines. [3] Menadione at 1-20 mM dose- and time-dependently inhibits cell proliferation of AR4-2J cells. Menadione (100 mM) causes rapid cell death. Menadione (100 mM) induces DNA smear in electrophoresis indicative of necrosis, while lower concentrations (10-20 mM) induces a DNA ladder indicative of apoptosis. Menadione at 1-20 mM induces wild-type P53, whereas the 100 mM menadione has a minor effect on wild-type P53. [4] Menadione treatment clearly affects the mitochondrial function of Jurkat T cells by inducing a collapse of the inner transmembrane potential (DeltaPsi(m)) and a decrease in inner membrane mass, which could be completely reversed by N-acetylcysteine. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT22 cells MnHrSpVv[3Srb36gZZN{[Xl? NHnZdJNP\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgbY4hdW:3c3WgTHQzOiClZXzsd{Bie3Onc4Pl[EBieyCycnX2[Y51cW:wIH\yc40h\2y3dHHtZZRmNWmwZIXj[YQhd3irZHH0bZZmKGOnbHyg[IVifGhiYomgUXRUKGG|c3H5MEBRTDVyPUCuO|k4KM7:TR?= MlvJNlM{Ojd2Nki=
HeLa cell M4rGNWN6fG:2b4jpZ4l1gSCjc4PhfS=> NYD3[lVtS3m2b4TvfIlkcXS7IHHnZYlve3RiSHXMZUBk\WyuIHzpcoV{NCCHQ{WwQVMvPyEQvF2= MkLmNVU{Pjl2MEC=
PaCa2 cell M3PFVXBzd2yrZnXyZZRqd25iYYPzZZk> NGD6UJFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIIDhcoNz\WG2aXOgZ4Fz[2mwb33hJG1q[SCSYVPhNkBk\WyuIHzpcoUh[nliY3;sc5JqdWW2cnnjJIF{e2G7LDDJR|UxRTZwMjFOwG0> MYOxOlIyPjVyMB?=
HT-29 cells NUfyZWhMS3m2b4TvfIlkcXS7IHHzd4F6 M1vNRlczKGh? Ml3JR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF06NjZ7IN88US=> NInGOFIzOzd7MUO2Oy=>
DU145 cells NHzucodEgXSxdH;4bYNqfHliYYPzZZk> M33KfFczKGh? NH24SWJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTlwOE[g{txO MoPsNlM4QTF|Nke=
MDA-MB-231 cells NIHTb4xEgXSxdH;4bYNqfHliYYPzZZk> M1THZlczKGh? MlPHR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyPUGyMlY5KM7:TR?= M3LHfVI{PzlzM{[3
human A2058 cells M{\MZmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2HIUmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzODV6IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4Yh\m:{bXH6ZY4h\m:{bXH0bY9vKGG2IHThfUA2KGK7IH3lZZN2emmwZzDhZpNwemKjbnPlJIF1KDR|MDDucUwhUUN3ME2xN{41KM7:TR?= NHK0[okyQTl4OUSwNC=>
SAS cells M1P3b2N6fG:2b4jpZ4l1gSCjc4PhfS=> NH3HWGY1QCCq MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTRXMh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVQh|ryP MWOyOFkyOzdzMh?=
HaCaT cells MorqSpVv[3Srb36gZZN{[Xl? NYXHeIVXPDhiaB?= NG\vXYpCdnSraInw[ZJxem:uaX\ldoF1cX[nIHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hUGGFYWSgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iClZXzsJIdzd3e2aDDh[pRmeiB2ODDodpMh[nlicHjhd4Uh[2:wdILhd5QhdWmlcn;zZ49xgSxiSVO1NF0yPS56IN88US=> NIrRNWQzPDl4NEK0Oi=>
HeLa cells NI\t[ZFEgXSxdH;4bYNqfHliYYPzZZk> NY\TO2RGPDhiaB?= MnLKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yQCEQvF2= NUj1Voo1OjR7MUO3NVI>
CRL2796 cells MV3DfZRwfG:6aXPpeJkh[XO|YYm= NEXpV2FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEWkx{N{m2JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiZn;ycYF7[W5iZn;ycYF1cW:wIHH0JIRigSB2IHL5JI1m[XO3cnnu[{Bi[nOxcnLhcoNmKGG2IESzNEBvdSxiSVO1NF0zOSEQvF2= MXuxPVk3QTRyMB?=
HL60 cells M{PIOWZ2dmO2aX;uJIF{e2G7 NIfCd4c2OCEQvF2= M1XxUmlv\HWldHnvckBw\iCwZXPyc5NqeyCrbjDpcpRi[3RiaIXtZY4hUEx4MDDj[YxteyCjc4Pld5Nm\CCjczDjfZRwe2:uaXOgUGRJKHKnbHXhd4Uh[XRiNUCgeW0> MUWyOVQ3OjJ5Mx?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-JNK / JNK / p-c-Jun / c-Jun / p-ERK / ERK ; 

PubMed: 20578144     

Immunoblots of total protein from VEC and siAtg5 cells treated with 50 μM menadione for the times shown and probed for phospho-JNK (P-JNK), total JNK, phospho-c-Jun (P-c-Jun), total c-Jun, phospho-ERK1/2 (P-ERK1/2) and total ERK1/2.

Cyclin B1 / CDK1 / CDC25C ; 

PubMed: 28077999     

AGS cells were treated with indicated dose of menadione (0, 5, 10, 15 μM) for 24 h. A: The cell lysates were subjected to Westernblot for CyclinB1, CDK1, and CDC25C. 

c-Fos / HO-1 ; 

PubMed: 25414256     

Cells were exposed to 50 μm menadione for the indicated time and subjected to Northern blot analysis of c-Fos and Ho-1. GAPDH was used as a loading control. D and E, involvement of p53 in the regulation of ERK1/2 phosphorylation. 

20578144 28077999 25414256


Solubility (25°C)

In vitro DMSO 34 mg/mL (197.46 mM)
Ethanol 34 mg/mL (197.46 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 172.18


CAS No. 58-27-5
Storage powder
in solvent
Synonyms Vitamin K3
Smiles CC1=CC(=O)C2=CC=CC=C2C1=O

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID