Name: Epirubicin (IMI 28) HCl
Brand: Selleck Chemicals
SKU: S1223
Rating: 5 (23 reviews)

Epirubicin (IMI 28) HCl Chemical Structure

CAS No. 56390-09-1

Selleck's Epirubicin (IMI 28) HCl has been cited by 23 publications

Purity & Quality Control

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Topoisomerase Signaling Pathway Map

Biological Activity

Description

Epirubicin HCl (IMI 28, NSC 256942, 4'-epidoxorubicin), a semisynthetic L-arabino derivative of doxorubicin, is an antineoplastic agent by inhibiting Topoisomerase. Epirubicin induces apoptosis.

Targets

Topoisomerase ^[1]
(Cell-free assay)

In vitro

Epirubicin, like doxorubicin, exerts its antitumor effects by complex with DNA, resulting in damage to DNA and interference with the synthesis of DNA, RNA, and proteins. Epirubicin may also affect the integrity and activity of cellular membranes. Maximal cell kill caused by Epirubicin occurs during the S phase of the cell cycle. With higher concentrations effects are also seen in early G2 as well as G1 and M phases. ^[1] Epirubicin display antineoplastic activity against most cancer cells. Epirubicin is cytotoxic to Hepatoma G2 cells with IC50 of 1.6 μg/mL at 24hr. 1.6 μg/mL Epirubicin induces apoptosis of Hep G2 cells, and higher activity of catalase by 50%, Se-dependent glutathione peroxidase by 110%, Cu, Zn-superoxide dismutase by 172% and Mn-superoxide dismutase by 135%. Epirbicin increases the cellular expression of NADPH-CYP 450 reductase, and reduces GST-π expression. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

NCI-H460	M2DNbGN6fG:2b4jpZ4l1gSCjc4PhfS=>		NV7aZ2V{PDhiaILz	NHGxWnlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSES2NEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxRTBwMENOwG0v	Mnr4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Nke5O\|UoRjJ7N{[3PVc2RC:jPh?=
HepG2	M3HOTWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NVrsUFlnPDhiaILz	NHH4Z5VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOc7:TT6=	Mo[0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Nke5O\|UoRjJ7N{[3PVc2RC:jPh?=
MCF10A	NVfZ[YNOS3m2b4TvfIlkcXS7IHHzd4F6		Mo[5OFghcHK\|	MnnFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHOTCDIHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4y\|ryPLh?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd4N{m3OUc,Ojl5Nke5O\|U9N2F-
NCI-H460	MkPrR5l1d3SxeHnjbZR6KGG\|c3H5		MkLlOFghcHK\|	NV\rcpIxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVkOLLVi0OlAh[2WubIOgZZN{\XO\|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[W[2ZYKgOFghcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlEz\|ryPLh?=	NHPZSWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEGxPVAzPid-MkixNVkxOjZ:L3G+
NCI-H460	NYDlelBoS3m2b4TvfIlkcXS7IHHzd4F6		MlTyO\|IhcHK\|	MUnDfZRwfG:6aXPpeJkhcW5iaIXtZY4hVkOLLVi0OlAh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlEz\|ryPLh?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTJ3MU[yNUc,OzF{NUG2NlE9N2F-
MCF10A	NF:2RnFEgXSxdH;4bYNqfHliYYPzZZk>		NXO2cJZQPDhiaILz	NUDPNW04S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOIMUDBJINmdGy\|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6xN:69VS5?	NVXiV21PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixNVkxOjZpPkK4NVE6ODJ4PD;hQi=>
MCF-7	NXPz[WlyTnWwY4Tpc44h[XO\|YYm=			MlHlR49ueG:3bnSge4F{KHSnc4Tl[EBnd3JiaYTzJJRwgGmlaYT5JIFo[Wmwc4SgbJVu[W5iYoLlZZN1KGOjbnPldkBk\WyuczjNR2YuPyluIFnDOVA:OC5{zszNMi=>	MlvjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTV2OEiyNEc,QTV2OEiyNFww[T5?
MDA-MB-435	NXP4THBYS3m2b4TvfIlkcXS7IHHzd4F6		NUPDbGJOPDhiaILz	MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy\|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6yOu69VS5?	M2P6[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUG5NFI3Lz5{OEGxPVAzPjxxYU6=
MDA-MB-435	NYfzUJoyS3m2b4TvfIlkcXS7IHHzd4F6		M{XyZlczKGi{cx?=	MV\DfZRwfG:6aXPpeJkhcW5iaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliaX7jeYJifGWmIH\vdkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOjcQvF2u	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTJ3MU[yNUc,OzF{NUG2NlE9N2F-
HepG2	NIfyfm1EgXSxdH;4bYNqfHliYYPzZZk>		MYW0PEBpenN?	NYW0VFR7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkOy{txONg>?	MorlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhzMUmwNlYoRjJ6MUG5NFI3RC:jPh?=
HCT116	M2HXTmN6fG:2b4jpZ4l1gSCjc4PhfS=>		MkDwOFghcHK\|	NUHNbFMyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEOWMUG2JINmdGy\|IHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;MD6zO:69VS5?	M3vOVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MUG5NFI3Lz5{OEGxPVAzPjxxYU6=
HCT116	MorPR5l1d3SxeHnjbZR6KGG\|c3H5		M4XaXFczKGi{cx?=	M1\PdGN6fG:2b4jpZ4l1gSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlM4\|ryPLh?=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTJ3MU[yNUc,OzF{NUG2NlE9N2F-
HCT116	NVHPe3VVS3m2b4TvfIlkcXS7IHHzd4F6		MXO0PEBpenN?	NFzoWYpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjUQvF2u	M3TYWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7N{[3PVc2Lz5{OUe2O\|k4PTxxYU6=
HeLa	NGTQUIlEgXSxdH;4bYNqfHliYYPzZZk>		MWe3NkBpenN?	MYHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIF{e2W\|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE41Os7:TT6=	MnTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3NkC2PVUoRjJ5NU[wOlk2RC:jPh?=
PC3	MlLhR5l1d3SxeHnjbZR6KGG\|c3H5		NW\oWHBqPiCmYYnz	NUCwXW57S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN\|IHPlcIx{KGGodHXyJFYh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9NE41Ps7:TT6=	Ml;kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{N{[2O\|koRjJ{Mke2Olc6RC:jPh?=
HEK293T	M1TsPGN6fG:2b4jpZ4l1gSCjc4PhfS=>		M3rPblczKGi{cx?=	M2fSS2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N3Qh[2WubIOgZZN{\XO\|ZXSgZZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgO\|IhcHK\|IHL5JG1VWyCjc4PhfUwhUUN3ME2wMlQ4\|ryPLh?=	MkPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3NkC2PVUoRjJ5NU[wOlk2RC:jPh?=
A549	NYTJbGg4S3m2b4TvfIlkcXS7IHHzd4F6		MWm3NkBpenN?	M4LKU2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO\|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0xNjZ\|zszNMi=>	NVXQWo1URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1OlA3QTVpPkK3OVYxPjl3PD;hQi=>
MDA-MB-435	MVLDfZRwfG:6aXPpeJkh[XO\|YYm=		MXq0PEBpenN?	NYnsNohkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyPUCuO:69VS5?	NFzVXJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe2O\|k4PSd-Mkm3Olc6PzV:L3G+
MCF7	NX3GbIlES3m2b4TvfIlkcXS7IHHzd4F6		Mm[1O\|IhcHK\|	NVPPbmhJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG\|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR\|UxRTBwN{JOwG0v	NHLRU4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{W2NFY6PSd-Mke1OlA3QTV:L3G+
HCT116	MkixR5l1d3SxeHnjbZR6KGG\|c3H5		NWi2SHRxPiCmYYnz	MVjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOkBl[Xm\|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlgz\|ryPLh?=	NF;UNo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK3OlY4QSd-MkKyO\|Y3Pzl:L3G+
MDA-MB-231	NXfuTFB1S3m2b4TvfIlkcXS7IHHzd4F6		NEfWfmY1QCCqcoO=	MW\DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC57zszNMi=>	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd4N{m3OUc,Ojl5Nke5O\|U9N2F-
Hep3B	NFL0fHZEgXSxdH;4bYNqfHliYYPzZZk>		NFzsVJY3KGSjeYO=	NHy3PWhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\XB\|QjDj[YxteyCjZoTldkA3KGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvQTcQvF2u	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ5Nk[3PUc,OjJ{N{[2O\|k9N2F-
HepG2	NYDHbHU6S3m2b4TvfIlkcXS7IHHzd4F6		NIfZZZY4OiCqcoO=	NXTUVJY3S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1zLkROwG0v	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzV4ME[5OUc,Ojd3NkC2PVU9N2F-
HepG2	MU\DfZRwfG:6aXPpeJkh[XO\|YYm=		MYS2JIRigXN?	M{nPNWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFYh\GG7czDifUBOXFRiYYPzZZktKEmFNUC9NU43Pc7:TT6=	NGjV[YE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK3OlY4QSd-MkKyO\|Y3Pzl:L3G+
SNB19	M2fXNWN6fG:2b4jpZ4l1gSCjc4PhfS=>		NF3iWXk4OiCqcoO=	NEflcpdEgXSxdH;4bYNqfHliaX6gbJVu[W5iU17CNVkh[2WubIOgZZN{\XO\|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgO\|IhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3ME2yMlPPxE1w	NWLmfmoyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyOVE3OjFpPkOxNlUyPjJzPD;hQi=>
MCF7	MmTMRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M1Kz[FQ5KGi{cx?=	MkLvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPQ1[3JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Oy55zszNMi=>	NHfm[lI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
NIH/3T3	MoPYRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NF\1T4k1QCCqcoO=	NUnVcldNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W\|ZTDOTWgwO1R\|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9OE4z\|ryPLh?=	Ml3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OEewOVcoRjJ\|Mki3NFU4RC:jPh?=
T47D	MkH5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NEPUOlg1QCCqcoO=	NEfG[mJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGS0O2Qh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD12LkROwG0v	M2\3UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki3NFU4Lz5{M{K4O\|A2PzxxYU6=
HepG2	MXfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MVG0PEBpenN?	NGDCdldCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO\|YYmsJGlEPTB;ND62{txONg>?	NFPsfIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
SGC7901	NEnuVYZCdnSrY3HuZ4VzKGG\|c3H5		NYrNSnRZPzJiaILz	NVuzfZZvSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJHNISzd7MEGgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF02NjF4zszNMi=>	NHvlT4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmwN\|Y3Oid-Mkm5NFM3PjJ:L3G+
MDA-MB-231	NHXiSo9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NXr5eXNnPDhiaILz	NH\6NGhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF02NjcQvF2u	NHzNWpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4O\|A2Pyd-MkOyPFcxPTd:L3G+
MDA-MB-231	MWHDfZRwfG:6aXPpeJkh[XO\|YYm=		NXzBUIgxPzJiaILz	MY\DfZRwfG:6aXPpeJkhcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliaX7jeYJifGWmIH\vdkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVUvPs7:TT6=	NHT6S\|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MUK1NVYzOSd-M{GyOVE3OjF:L3G+
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DU145	M3HqS2FvfGmycn;sbYZmemG2aY\lJIF{e2G7		M124d\|Q5KGi{cx?=	MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESXMUS1JINmdGy\|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:QS57zszNMi=>	M{\tbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki3NFU4Lz5{M{K4O\|A2PzxxYU6=
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A673	M4LndZFJXFNiYYPzZZk>			M3vGdpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy\|	Ml;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	MYLxTHRUKGG\|c3H5			M12x[ZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz	MoC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-37	NEX2WotyUFSVIHHzd4F6			MWTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN?	M{DuXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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Saos-2	Mn34dWhVWyCjc4PhfS=>			MlHGdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?=	NVfPelA3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
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NB1643	MX;xTHRUKGG\|c3H5			MlTTdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?=	NGqwdXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
LAN-5	NH;ydVFyUFSVIHHzd4F6			NXX5NVU3eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz	Mm[2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
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Rh18	MlPQdWhVWyCjc4PhfS=>			M{W5TZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
OHS-50	NW\k[\|E3eUiWUzDhd5NigQ>?			NHPiemdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzz	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
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NB1643	NIG4WWZyUFSVIHHzd4F6			MlLvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR\|IHPlcIx{	Mlr6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
A673	NUXQc21jeUiWUzDhd5NigQ>?			NHHWRVVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd\|IHPlcIx{MQ>?	MnH3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC	MWLxTHRUKGG\|c3H5			Mk[5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>?	MlnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	NH7uV4xyUFSVIHHzd4F6			M2jwUJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LAN-5	MnXMdWhVWyCjc4PhfS=>			NV:5NXRIeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN?	MnnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
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SK-N-SH	M3TrWJFJXFNiYYPzZZk>			MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBQenSqb3fvcoFtKDOGII\pZYJqdGm2eTDzZ5Jm\W5iZn;yJHNMNU5vU1igZ4VtdHN?	M3m2XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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TC32	M4GzbZFJXFNiYYPzZZk>			NHnCOIFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFSFM{KgZ4VtdHN?	MkPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R)	MnvIdWhVWyCjc4PhfS=>			NE\PbWdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy\|	Mo\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
RD	MlXZdWhVWyCjc4PhfS=>			NH;TdmlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
SGC7901	NUewTWRUTnWwY4Tpc44h[XO\|YYm=	MX2xJIFv\CBzMDD1US=>	NF32ZoMzPCC2bzC3NkBpenN?	MVrJcoR2[3Srb36gc4YhdW:{cHjvcI9ocWOjbDDjbIFv\2W\|IHnuJIh2dWGwIGPHR\|c6ODFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJINmdGxiYoLlZYsh[XRiMTDhcoQhOTBidV2gZYZ1\XJiMkSgeI8hPzJiaILzJIJ6KGmwdnXyeIVlKG2rY4Lvd4NweGmlIHHuZYx6e2m\|	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTlyM{[2Nkc,Ojl7MEO2OlI9N2F-
C6	MVLD[YxtKGO7Y3zlJIF{e2G7		MYWyOEBpenN?	MnO3R4VtdCCleXPs[UBienKnc4SgbY4hemG2IFO2JINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGOnbHygdI9xfWyjdHnvckBifCC\|dXKgS\|AheGijc3WgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdiYnHz[YQh\myxdzDjfZRwdWW2comgZY5idHm\|aYO=	M1;ZOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNES3NFgzLz5\|MUS0O\|A5OjxxYU6=
C6	M4G5OGNmdGxiY4njcIUh[XO\|YYm=		NHj4N\|MzPCCqcoO=	M3WwRmNmdGxiY4njcIUh[XK{ZYP0JIlvKHKjdDDDOkBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiClZXzsJJBweHWuYYTpc44h[XRiR{KvUUBxcGG\|ZTDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nlicILvdIllcXWvIHnv[Ill\SC\|dHHpcolv\yCkYYPl[EBndG:5IHP5eI9u\XS{eTDhcoFtgXOrcx?=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTR2N{C4Nkc,OzF2NEewPFI9N2F-
C6	MnfrR4VtdCCleXPs[UBie3OjeR?=		MUOyOEBpenN?	MlPIR4VtdCCleXPs[UBienKnc4SgbY4hemG2IFO2JINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygdI9xfWyjdHnvckBifCCVIIDoZZNmKG2nYYP1doVlKGGodHXyJFI1KGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJie2WmIH\sc5ch[3m2b33leJJ6KGGwYXz5d4l{	M2DIV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNES3NFgzLz5\|MUS0O\|A5OjxxYU6=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	PARP / E2F1 / p-E2F1 / p-MK2 / p-Chk2 ; E2F1 / p-p53 / p53 / Caspase-7 ; FOXM1 / Cyclin B1 / PLK ; HIF-1α / GLUT1 / PDK1	22802261 21518729 28339028
Growth inhibition assay	Cell death	24158003

In vivo

Epirubicin are clinically active against a broad range of tumor types, including breast cancer, malignant lymphomas, soft tissue sarcomas, lung cancer, pleural mesothelioma, gastrointestinal cancer, head and neck cancer, ovarian cancer, prostatic carcinoma, transitional bladder carcinoma and so on. ^[3] Epirubicin at a dose of 3.5 mg/kg suppresses tumor mass of human breast tumor xenograft R-27 by 74.4 %. ^[4]

Protocol (from reference)

Cell Research:^[2]

Cell lines: Human hepatocellular carcinoma cell Hep G2
Concentrations: 0.05-12 μg/mL
Incubation Time: 1 days
Method: Hep G2 cells (500 cells/well, monolayer) are plated in a 96-well plate. The next day the cells are treated with Epirubicin in the medium. At the end of the incubation periods, 15% volume of MTT dye solution is added. After 1 hr of incubation at 37℃, an equal volume of solubilization/stop solution (dimethylsul-foxide) is added to each well for an additional 1 hr incubation. The absorbance of the reaction solution at 570 nm is recorded.
(Only for Reference)

Animal Research:^[4]

Animal Models: Human breast tumor xenograft R-27
Dosages: 3.5 mg/kg
Administration: i.v. every 4 day for 3 times
(Only for Reference)

References

[4] Asanuma F, et al. Folia Microbiol (Praha), 1998, 43(5), 473-474.

+ Expand

Solubility (25°C)

In vitro	DMSO	100 mg/mL (172.41 mM)
	Water	100 mg/mL warmed (172.41 mM)
	Ethanol	Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	579.98
Formula	C₂₇H₂₉NO₁₁.HCl
CAS No.	56390-09-1
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)CO)O)N)O.Cl

Download Epirubicin (IMI 28) HCl SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05128617	Recruiting	Drug: Epirubicin-cyclophosphamide\|Drug: Paclitaxel	Breast Cancer	Institut de cancérologie Strasbourg Europe\|Université de Strasbourg - Unité de Recherche 3072 - Mitochondries Stress oxydant Protection musculaire	November 4 2021	--
NCT01740271	Recruiting	Drug: Epirubicin	Breast Neoplasms	AHS Cancer Control Alberta	December 2012	Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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