Decitabine (NSC 127716)

Catalog No.S1200 Synonyms: Deoxycytidine, Dacogen, 5-aza-2'-deoxycytidine, 5-AZA-dC, 5-aza-CdR

For research use only.

Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR) is a DNA methyltransferase inhibitor, incorporating into DNA and resulting in hypomethylation of DNA and intra-S-phase arrest of DNA replication. It is used to treat myelodysplastic syndrome (MDS). Decitabine induces cell cycle arrest and apoptosis in various cancer cell lines.

Decitabine (NSC 127716) Chemical Structure

CAS No. 2353-33-5

Selleck's Decitabine (NSC 127716) has been cited by 134 publications

Purity & Quality Control

Choose Selective DNA Methyltransferase Inhibitors

Biological Activity

Description Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR) is a DNA methyltransferase inhibitor, incorporating into DNA and resulting in hypomethylation of DNA and intra-S-phase arrest of DNA replication. It is used to treat myelodysplastic syndrome (MDS). Decitabine induces cell cycle arrest and apoptosis in various cancer cell lines.
Targets
DNA methylation [1]
(HL-60, KG1a cells)
In vitro

Decitabine inhibits cell growth in a dose and time-dependent manner with IC50 of approximately 438 nM and 43.8 nM for 72 hours and 96 hours exposure in HL-60 and KG1a leukemic cells, respectively. [1] A recent study shows that Decitabine exhibits high anti-proliferative and pro-apoptotic activity against anaplastic large cell lymphoma (ALCL), and inhibits [3H]–thymidine uptake in KARPAS-299 cells with EC50 of 0.49 μM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Eca109 M2\PXWZ2dmO2aX;uJGF{e2G7 NXTzTmtFOC53wrFOwG0> NEfnS|IzPCCq Mmnle4F1\XJ? NGC3T5Vud2S3bHH0[ZMhfGinIHflcoUh\XiycnXzd4lwdiCxZjDNRWdGNUFibXXtZoVzew>? NVy5SXY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlMxQDJpPkK1NVI{ODh{PD;hQi=>
Eca109 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DTNVAvPS9{LkWvOUDPxE1? MWmyOE81QC95MjDo NXrGclJrf2G2ZYK= MULpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJIRwe2VvIHHu[EB1cW2nLTDk[ZBmdmSnboSgcYFvdmW{ NFfJWYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN|A5Oid-MkWxNlMxQDJ:L3G+
Eca109 MYjGeY5kfGmxbjDBd5NigQ>? MkfzNE42yqEQvF2= NWXObG5tPi9zMj:yOEBp M{XIUpdifGW{ Mo\TbY5pcWKrdIOgZ4VtdCCvaXfyZZRqd28EoB?= NHzaS5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN|A5Oid-MkWxNlMxQDJ:L3G+
Eca109 M{n1e2Z2dmO2aX;uJGF{e2G7 NIXpcnAxNjYEoN88US=> MV6yOEBp NIC0cG54[XSnch?= MYLpcohq[mm2czDj[YxtKGmwdnHzbY9v M1PXcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUKzNFgzLz5{NUGyN|A5OjxxYU6=
Eca109 Mk\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXHXHRjOC53wrFOwG0> M{\V[lI1KGh? MXj3ZZRmeg>? NVL1ZXg{cW6mdXPld{BIOi:PIHHydoV{fCCrbjD0bIUh[2WubDDjfYNt\Q>? NX;OVJEzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlMxQDJpPkK1NVI{ODh{PD;hQi=>
Eca109 NGfHNFRHfW6ldHnvckBCe3OjeR?= NWfrXFR3OC53L{Gg{txO NEfkenIzPCCq M3fq[5difGW{ NYTQXZFW\GWlcnXhd4V{KGW6cILld5Nqd25ib3[gUmYu|rqEMjDhcoQhVU2SMh?= NGX6R4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN|A5Oid-MkWxNlMxQDJ:L3G+
SW1116  MlnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzlNE42NzFxMj:1JO69VQ>? NX7hWno6PDhiaB?= M2PJZWROW09? NG\RXWtmdmijbnPld{B1cGViR3XmbZRqdmmkIHnu[JVk\WRiY3XscEBqdmirYnn0bY9v NH3BNlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O|QzQDZ:L3G+
LOVO MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVj5TYc6OC53L{GvNk82KM7:TR?= NFzNb2w1QCCq NIHRbo1FVVOR Mmj6[Y5p[W6lZYOgeIhmKEenZnn0bY5q[iCrbnT1Z4VlKGOnbHygbY5pcWKrdHnvci=> MkP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6N{SyPFYoRjJ2OEe0Nlg3RC:jPh?=
SW1116  MV7GeY5kfGmxbjDBd5NigQ>? NIjKRpUyOCEQvF2= MVS0PEBp MUfEUXNQ NIH1OZJqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5NEK4Okc,OjR6N{SyPFY9N2F-
LOVO NF\WdJZHfW6ldHnvckBCe3OjeR?= M2LlRlExKM7:TR?= MUS0PEBp Mn6wSG1UVw>? NHexNGhqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ? M4\HSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
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LOVO NE\1UHlCeG:ydH;zbZMhSXO|YYm= M4jFdFExKM7:TR?= MYG0PEBp MlrBSG1UVw>? M{fyUoVvcGGwY3XzJGdm\mm2aX7pZk1qdmS3Y3XkJIFxd3C2b4Ppdy=> NYrkVVk6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O|QzQDZpPkK0PFc1Ojh4PD;hQi=>
RPMI-8226 MYDBdI9xfG:|aYOgRZN{[Xl? M{\wfFEwOiEQvF2= NX;6N2F4PDhxN{KvPVYhcA>? M3;1eGROW09? M1\vbIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
OPM-2  M3jBVmFxd3C2b4Ppd{BCe3OjeR?= MVyxM|Ih|ryP MYS3Nk86Pi9zMkCgbC=> NWjGelI5TE2VTx?= MlPxbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NITT[449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN|ExQCd-MkS4N|MyODh:L3G+
JJN3  M{T1eWFxd3C2b4Ppd{BCe3OjeR?= MWWwMlUwOSEQvF2= M3PS[FI1NzR6IHi= NX;PWXhUTE2VTx?= NWHjdox1cW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
NCI-H929  NEL6ZVRCeG:ydH;zbZMhSXO|YYm= MnLGNU8zKM7:TR?= Mnj4O|IwQTZxMUKwJIg> NHTmSXRFVVOR NWXW[IhPcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
RPMI-8226 NFTLWoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnvS20yNzJizszN MmjlNlQwPDhxN{KgbC=> NEjwdI1FVVOR MUPh[oZm[3S|IHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44hdmWpYYTpeoVtgQ>? MlfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6M{OxNFgoRjJ2OEOzNVA5RC:jPh?=
OPM-2  MoS3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlm4NU8zKM7:TR?= MVWyOE81QC95MjDo M4W5NmROW09? NVTQRWY4[W[oZXP0d{Bk\WyuIHP5Z4xmKHC{b3fy[ZN{cW:wIH7l[4F1cX[nbIm= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh|M{GwPEc,OjR6M{OxNFg9N2F-
JJN3  MmTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIWwNGUxNjVxMTFOwG0> M2\ke|I1NzR6L{eyJIg> MVjEUXNQ MX\h[oZm[3S|IHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44hdmWpYYTpeoVtgQ>? M1LpdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN|ExQDxxYU6=
NCI-H929  NWq1R2N1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPyNU8zKM7:TR?= MYmyOE81QC95MjDo NUfvdHEzTE2VTx?= MnfvZYZn\WO2czDj[YxtKGO7Y3zlJJBzd2e{ZYPzbY9vKG6nZ3H0bZZmdHl? M1zxdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN|ExQDxxYU6=
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CD4+ CD25− T  MYHGeY5kfGmxbjDBd5NigQ>? NUTPcZU3OS93IN88US=> MnzodoVlfWOnUzDncI9j[WxiRF7BJI1mfGi7bHH0bY9v NEfUO3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3OlM3OCd-MkS0O|Y{PjB:L3G+
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ML-1 MXrBdI9xfG:|aYOgRZN{[Xl? MUmwMlI2NzBwNT:wMlc2NzFizszN NXXVNJc3PDhxN{KvPVYhcA>? NV\1RmExyqCSQmO= MoPhbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HL-60 MkXORZBweHSxc3nzJGF{e2G7 NWHrSWFzOC5{NT:wMlUwOC55NT:xJO69VQ>? NVjXc|R4PDhxN{KvPVYhcA>? MnH3xsBRSlN? MoDrbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
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HL-60 NGTHN4JHfW6ldHnvckBCe3OjeR?= M2D0N|I2OC93MEDuUS=> M3\CZ|Q5KGh? MVlCpHBDWw>? M{DnV4lv\HWlZYOg[IVt[XmnZDDhcoQhe3W|dHHpcoVlKFKRUzDpcoNz\WG|ZR?= M1LRZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN|YyOzxxYU6=
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HL-60 MmrPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV6xJO69VQ>? NV7afGkzPDhiaB?= NVT6PWxicW6lcnXhd4V{KEd{LYDoZZNmKGOnbHyg[pJi[3Srb36= NHXa[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECwNFMzPCd-MkSwNFA{OjR:L3G+
MDA‑MB‑453 NYrTZpRtTnWwY4Tpc44hSXO|YYm= M4nhZVAvOi9zIN88US=> NHS5NZM4OiCq NGHkb2dk[XW|ZYOgdoUu\XiycnXzd4lwdiCxZjDjcIF2\GmwwrCxxsA> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NEKyPEc,OjN6NESyNlg9N2F-
HCC1569 M1LPbmZ2dmO2aX;uJGF{e2G7 MWqwMlIwOSEQvF2= NID1TYs4OiCq MXXjZZV{\XNicnWt[ZhxemW|c3nvckBw\iClbHH1[IlvyqBzwrC= NF30THk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OFIzQCd-MkO4OFQzOjh:L3G+
BT‑474 NFLlRZhHfW6ldHnvckBCe3OjeR?= NU[0bFRoOC5{L{Gg{txO NET3bpA4OiCq NFfoblBk[XW|ZYOgdoUu\XiycnXzd4lwdiCxZjDjcIF2\GmwwrCxxsA> NWK1RnZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFQzOjhpPkKzPFQ1OjJ6PD;hQi=>
AGS NGfCS3lCeG:ydH;zbZMhSXO|YYm= NH7XW5g2NzFyL{KwM|UxKM7:TR?= NWq3cWFCPDkEoHlCpC=> M2LWcmROW09? M4LPU4lvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0feKh M3zlR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUiyO|g1Lz5{M{W4Nlc5PDxxYU6=
A549 M2XmZmFxd3C2b4Ppd{BCe3OjeR?= NGTLZlg2NzFyL{KwM|UxKM7:TR?= MX60POKhcMLi Mlv5SG1UVw>? MlXDbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5xsA> M2OwXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUiyO|g1Lz5{M{W4Nlc5PDxxYU6=
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Kasumi-1 NF;TNJBCeG:ydH;zbZMhSXO|YYm= Mnv1NE42KM7:TR?= MkfBOFjDqGkEoB?= NEPWe49l\WO{ZXHz[ZMhfGinIHPlcIwhfmmjYnnsbZR6KGOxLYTy[YF1\WRid3n0bEBV\i2QUD3zZy=> MnLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUOzOFgoRjJ|NEmzN|Q5RC:jPh?=
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MOLT4/DNR M2O5NWZ2dmO2aX;uJGF{e2G7 MnywOUDPxE1? MUm0JIQ> M2D2bJJm\HWlZYOgRWJESjFibWLORUBmgHC{ZYPzbY9v NUW3U2M5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
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MOLT4/DNR MljTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEKxN4E2KM7:TR?= NH3nSog1KGR? MVXy[YR2[2W|IITo[UBKSzVywrD2ZYx2\SCob4Kg[IF2dm:{dXLpZ4lvKHOnboPpeIl3cXS7 NXzRZnZsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
Jurkat/DOX Mly5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTKOUDPxE1? MX:0JIQ> MXTy[YR2[2W|IITo[UBKSzVywrD2ZYx2\SCob4Kg[IF2dm:{dXLpZ4lvKHOnboPpeIl3cXS7 MojpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkC1O|AoRjJ|ME[wOVcxRC:jPh?=
ccRCC  NELmPJJCeG:ydH;zbZMhSXO|YYm= MWewMlAyNTFyzszN NUWzNINLPzJiaB?= MW\EUXNQ NWXqdplbcGG|IH3pcolu[WxiZX\m[YN1KG:wIHPlcIwheHKxbHnm[ZJifGmxbh?= M3jaOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK2OFY4Lz5{MkiyOlQ3PzxxYU6=
TNBC  MnHSRZBweHSxc3nzJGF{e2G7 MYGwMlAyNTFyzszN NVS1UGVFPzJiaB?= MlL2SG1UVw>? NVHZUoZmcGG|IH3pcolu[WxiZX\m[YN1KG:wIHPlcIwheHKxbHnm[ZJifGmxbh?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
A498 MmnlRZBweHSxc3nzJGF{e2G7 NHHkOHQxNjBzLUGw{txO NIDrcYw4OiCq MlToSG1UVw>? NH3Jc2VqdmS3Y3XzJJN6dmW{Z3nzeIlkKHKnc4DvcpNme8Lid3n0bEBzd22rZHXwd4lv MlKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Mk[0OlcoRjJ{OEK2OFY4RC:jPh?=
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MDA-231 MXvBdI9xfG:|aYOgRZN{[Xl? MVewMlAyNTFyzszN MVS3NkBp M2LYR2ROW09? NGjzRWZqdmS3Y3XzJJN6dmW{Z3nzeIlkKHKnc4DvcpNme8Lid3n0bEBzd22rZHXwd4lv MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
BT-20 NIq0NlRCeG:ydH;zbZMhSXO|YYm= Mn\ZNE4xOS1zMN88US=> NX75cWlsPzJiaB?= M3XicWROW09? NF3YbGRqdmS3Y3XzJJN6dmW{Z3nzeIlkKHKnc4DvcpNme8Lid3n0bEBzd22rZHXwd4lv NGTBOm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOlQ3Pyd-MkK4NlY1Pjd:L3G+
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MCF-7  MXjBdI9xfG:|aYOgRZN{[Xl? MX:xNEDPxE1? M1fNb|Q5KGh? NVvO[417emWmdXPld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NXvPUnB{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4PFc3QTdpPkKxPFg4Pjl5PD;hQi=>
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Assay
Methods Test Index PMID
Western blot Survivin / Bcl-2 / p53 / c-Myc / DNMT1 ; p-AKT / AKT / p-GSK3β / GSK3β / p-Myc / Myc / p-P70 / P70 / p-4EBP-1 / 4EBP-1 / PTEN ; phospho-p38 / p38 / phospho-NFκB / NFκB ; p-JAK1 / JAK1 / p-JAK2 / JAK2 / p-STAT3 / STAT3 ; E-cadherin / N-cadherin / Snail / MMP-2 / MMP-9 / Bcl-2 / Bax 26384351 25762617 26617834 26692933 28152502
Immunofluorescence DNMT1 ; E-cadherin / MMP-9 21303982 28152502
Growth inhibition assay Cell viability 26384351
In vivo In a ALK+ KARPAS-299 murine xenograft model, Decitabine at a dose of 2.5 mg/kg causes increased apoptosis and reduced proliferation of tumor cells, and also results in demethylation of tumor suppressor p16INK4A. [2]

Protocol (from reference)

Kinase Assay:[1]
  • DNA synthesis assay :

    The rate of DNA synthesis is measured by the incorporation of radioactive thymidine into DNA. HL-60 and KG1a cells are suspended in 2 mL RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 hours (drugs are added simultaneously). At 48 hours, 0.5 μCi [3H] thymidine (6.7 Ci/mmol) is added to each well and incubated for an additional 24 hours. The cells are placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA are then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using Beckman LS 6000IC scintillation counter. The IC50 is defined as the concentration of drug that inhibits 50% of the DNA synthesis of the leukemic cell lines from the dose–response curve.

Cell Research:[1]
  • Cell lines: HL-60 and KG1a
  • Concentrations: 0-500 nM
  • Incubation Time: 96 hours
  • Method: For the growth inhibition assay, cells in log phase are placed in 5 mL of medium. Different concentrations of Decitabine are added to the medium simultaneously. Cell counts are performed at the indicated times using a model ZM Coulter Counter. The concentration that produces 50% inhibition of growth (IC50) is determined from the growth curves of the drug treated leukemic cell lines.
Animal Research:[2]
  • Animal Models: KARPAS-299 human cells are inoculated subcutaneously into the right and left flanks of the mice.
  • Dosages: ≤2.5 mg/kg
  • Administration: Administered via i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
water
For best results, use promptly after mixing.

10mg/mL

Chemical Information

Molecular Weight 228.21
Formula

C8H12N4O4

CAS No. 2353-33-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C(C(OC1N2C=NC(=NC2=O)N)CO)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04985656 Withdrawn Drug: Pevonedistat|Drug: Decitabine|Drug: Cedazuridine Myelodysplastic Syndromes (MDS) Takeda October 1 2021 Phase 2
NCT04854889 Recruiting Drug: Decitabine Refractory Aplastic Anemia Institute of Hematology & Blood Diseases Hospital April 22 2021 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is S1200 a racemic mixture or a monomer?

Answer:
S1200 is R form

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