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Decitabine (NSC 127716)

Name: Decitabine (NSC 127716)
Brand: Selleck Chemicals
SKU: S1200
Rating: 5 (119 reviews)

Catalog No.S1200 Synonyms: Deoxycytidine, Dacogen, 5-aza-2'-deoxycytidine, 5-AZA-dC, 5-aza-CdR

For research use only.

Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR) is a DNA methyltransferase inhibitor, incorporating into DNA and resulting in hypomethylation of DNA and intra-S-phase arrest of DNA replication. It is used to treat myelodysplastic syndrome (MDS). Decitabine induces cell cycle arrest and apoptosis in various cancer cell lines.

Decitabine (NSC 127716) Chemical Structure

CAS No. 2353-33-5

Selleck's Decitabine (NSC 127716) has been cited by 119 publications

Purity & Quality Control

Choose Selective DNA Methyltransferase Inhibitors

Related Compound Libraries

Biological Activity

Description

Targets

DNA methylation ^[1]
(HL-60, KG1a cells)

In vitro

Decitabine inhibits cell growth in a dose and time-dependent manner with IC50 of approximately 438 nM and 43.8 nM for 72 hours and 96 hours exposure in HL-60 and KG1a leukemic cells, respectively. ^[1] A recent study shows that Decitabine exhibits high anti-proliferative and pro-apoptotic activity against anaplastic large cell lymphoma (ALCL), and inhibits [³H]–thymidine uptake in KARPAS-299 cells with EC50 of 0.49 μM. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Eca109	MVTGeY5kfGmxbjDBd5NigQ>?	MmGyNE42yqEQvF2=	NHK0PZEzPCCq	Mmfwe4F1\XJ?	NWLsZ4o3dW:mdXzheIV{KHSqZTDn[Y5mKGW6cILld5Nqd25ib3[gUWFITS2DIH3lcYJmenN?	M{XwN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUKzNFgzLz5{NUGyN\|A5OjxxYU6=
Eca109	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MonMNE42NzJwNT:1JO69VQ>?	MmfFNlQwPDhxN{KgbC=>	NVjZO3c3f2G2ZYK=	NUGwTWdLcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=>	MnXtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109	MXvGeY5kfGmxbjDBd5NigQ>?	MV2wMlXDqM7:TR?=	NXfYZplwPi9zMj:yOEBp	NUnCfpVlf2G2ZYK=	MXPpcohq[mm2czDj[YxtKG2rZ4LheIlwdsLi	MmPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109	MnTXSpVv[3Srb36gRZN{[Xl?	NIHNd5QxNjYEoN88US=>	NXPnbnI4OjRiaB?=	MVL3ZZRmeg>?	NV;iWYRWcW6qaXLpeJMh[2WubDDpcpZie2mxbh?=	NUT1N\|AxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlMxQDJpPkK1NVI{ODh{PD;hQi=>
Eca109	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmT4NE42yqEQvF2=	MWiyOEBp	Mo\pe4F1\XJ?	MVvpcoR2[2W\|IFeyM20h[XK{ZYP0JIlvKHSqZTDj[YxtKGO7Y3zl	MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109	NX[1UIwyTnWwY4Tpc44hSXO\|YYm=	NIiyZ4UxNjVxMTFOwG0>	NIDXXHEzPCCq	MXH3ZZRmeg>?	MWnk[YNz\WG\|ZYOg[ZhxemW\|c3nvckBw\iCQRj5OvmIzKGGwZDDNUXAz	NGPTe5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN\|A5Oid-MkWxNlMxQDJ:L3G+
SW1116	NXHWWI4{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{TGNlAvPS9zL{KvOUDPxE1?	M3XjdFQ5KGh?	NYf1VlhmTE2VTx?=	MX3lcohidmOnczD0bIUhT2WoaYTpcoljKGmwZIXj[YQh[2WubDDpcohq[mm2aX;u	NHXMUWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O\|QzQDZ:L3G+
LOVO	NXvx[YVWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NETqR2MxNjVxMT:yM\|Uh\|ryP	M3fJPVQ5KGh?	NI\FdGNFVVOR	MofP[Y5p[W6lZYOgeIhmKEenZnn0bY5q[iCrbnT1Z4VlKGOnbHygbY5pcWKrdHnvci=>	Mn\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6N{SyPFYoRjJ2OEe0Nlg3RC:jPh?=
SW1116	NUj0bmZwTnWwY4Tpc44hSXO\|YYm=	MnLONVAh\|ryP	NVG4NHFEPDhiaB?=	M2[2V2ROW09?	NH;uO\|NqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ?	NVmwdHBORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O\|QzQDZpPkK0PFc1Ojh4PD;hQi=>
LOVO	MUjGeY5kfGmxbjDBd5NigQ>?	MWexNEDPxE1?	NYO4[5lXPDhiaB?=	MljQSG1UVw>?	NIH2WWNqdmO{ZXHz[ZMhfGinIHXm[oVkfGm4ZTDheEBqdmirYnn0bY5oKEGNVDDhcoQhdVSRUjDzbYdv[WyrbnegdIF1cHejeYOgZ49u[mmwZXSge4l1cCCpZX\peIlvcWJ?	Mlm0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6N{SyPFYoRjJ2OEe0Nlg3RC:jPh?=
SW1116	MorwRZBweHSxc3nzJGF{e2G7	MmToNVAh\|ryP	MUW0PEBp	Moj2SG1UVw>?	MlTl[Y5p[W6lZYOgS4VncXSrbnniMYlv\HWlZXSgZZBweHSxc3nz	NIfsdHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O\|QzQDZ:L3G+
LOVO	MkDTRZBweHSxc3nzJGF{e2G7	MVuxNEDPxE1?	M{fmcVQ5KGh?	NGfhRoNFVVOR	MVHlcohidmOnczDH[YZqfGmwaXKtbY5lfWOnZDDhdI9xfG:\|aYO=	M1rUZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
RPMI-8226	NX\x[WVISXCxcITvd4l{KEG\|c3H5	MnzONU8zKM7:TR?=	MUW0PE84Oi97NjDo	NXnMUpJITE2VTx?=	MVzpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh\|M{GwPEc,OjR6M{OxNFg9N2F-
OPM-2	M2LGTmFxd3C2b4Ppd{BCe3OjeR?=	Mo\ZNU8zKM7:TR?=	NGPDW3k4Oi97Nj:xNlAhcA>?	NVnId2RXTE2VTx?=	NFXXSZpqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	M4DJRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
JJN3	MUnBdI9xfG:\|aYOgRZN{[Xl?	NELGSWMxNjVxMTFOwG0>	Mn;RNlQwPDhiaB?=	NUi5RVc1TE2VTx?=	Ml7ibY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	NIC2[Yo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN\|ExQCd-MkS4N\|MyODh:L3G+
NCI-H929	MVXBdI9xfG:\|aYOgRZN{[Xl?	MWCxM\|Ih\|ryP	NFi1c4s4Oi97Nj:xNlAhcA>?	NXjqRplDTE2VTx?=	NYnITlNtcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	M1LKUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
RPMI-8226	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MU[xM\|Ih\|ryP	M4S2[FI1NzR6L{eyJIg>	M2fs[2ROW09?	NEDFWG9i\m[nY4TzJINmdGxiY4njcIUheHKxZ4Lld5Nqd25ibnXnZZRqfmWueR?=	MlXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6M{OxNFgoRjJ2OEOzNVA5RC:jPh?=
OPM-2	NHvyXnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn7HNU8zKM7:TR?=	MmDyNlQwPDhxN{KgbC=>	MorqSG1UVw>?	MVfh[oZm[3S\|IHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44hdmWpYYTpeoVtgQ>?	M1zHTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
JJN3	M1\DTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYmwMlUwOSEQvF2=	MWOyOE81QC95MjDo	NHjiR5FFVVOR	NHnVem1i\m[nY4TzJINmdGxiY4njcIUheHKxZ4Lld5Nqd25ibnXnZZRqfmWueR?=	NEe0Ro09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN\|ExQCd-MkS4N\|MyODh:L3G+
NCI-H929	NWewTIRlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUexM\|Ih\|ryP	NXy2WVVkOjRxNEivO\|IhcA>?	MmrFSG1UVw>?	MXPh[oZm[3S\|IHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44hdmWpYYTpeoVtgQ>?	NEKzW5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN\|ExQCd-MkS4N\|MyODh:L3G+
HeLa	NWfx[GJJU2mwYYPlJGF{e2G7				Mkj5T4k:OTByMPMAl\|UxODBizszNJIZweiCqRV7UNS=>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd6MEC5PEc,OjR5OECwPVg9N2F-
HeLa	NV[zUXR4U2mwYYPlJGF{e2G7				MkPLT4k:PS54INMxJFAvPSEQvF2g[o9zKGiHTmSy	M3L1d\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa	M4HzPGtqdmG\|ZTDBd5NigQ>?				M1;uNmtqRTJzLk[gxtEhOy5yIN88UUBnd3JiaFPOWFE>	M1jjeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa	MnzLT4lv[XOnIFHzd4F6				MUHLbV0yPC52INMxJFQvPiEQvF2g[o9zKGiFTmSz	MknoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5OECwPVgoRjJ2N{iwNFk5RC:jPh?=
NB4	NV65RYxSTnWwY4Tpc44hSXO\|YYm=	MkLNNk42NzVxNz61M\|ExKM7:TR?=	Mn6yNlQhcA>?	M{jC[WROW09?	MV3pcoNz\WG\|ZXGgeIhmKGW6cILld5Nqd25ib3[gdJJm[3W{c3;yJI1qWi1zMkXh	NXfPfpNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PFQ5PzBpPkK0OFg1QDdyPD;hQi=>
CD4+ CD25− T	M2HCUmZ2dmO2aX;uJGF{e2G7	NWC5NpZ1OS93IN88US=>			MUTy[YR2[2WVIHfsc4JidCCGTlGgcYV1cHmuYYTpc44>	NHLaWGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3OlM3OCd-MkS0O\|Y{PjB:L3G+
BV-173	MXnBdI9xfG:\|aYOgRZN{[Xl?	M3uwNFAvOjVxMD61M\|AvPzVxMTFOwG0>	M1XD[VQ5Nzd{L{m2JIg>	NFfSNm3DqFCEUx?=	NFj4[\|VqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI>	NWTSUFNVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlM3OTNpPkK0OFI{PjF\|PD;hQi=>
ML-1	Mn;KRZBweHSxc3nzJGF{e2G7	M3jVd\|AvOjVxMD61M\|AvPzVxMTFOwG0>	MlTaOFgwPzJxOU[gbC=>	MkTjxsBRSlN?	NVrRT3YycW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBjd3SqIHTvd4UuKGGwZDD0bY1mNSCmZYDlcoRmdnRibXHucoVz	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HL-60	MlOyRZBweHSxc3nzJGF{e2G7	NU\UeIFGOC5{NT:wMlUwOC55NT:xJO69VQ>?	MkjwOFgwPzJxOU[gbC=>	NWnLfFVlyqCSQmO=	MmTYbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy	M3uzPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
KG-1a	M{nVSWFxd3C2b4Ppd{BCe3OjeR?=	NYTncIpSOC5{NT:wMlUwOC55NT:xJO69VQ>?	Mo\BOFgwPzJxOU[gbC=>	MXVCpHBDWw>?	M1PsOolv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=>	M4HMc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
BV-173	NH:2eHRHfW6ldHnvckBCe3OjeR?=	M{LQWFI2OC93MEDuUS=>	MYW0PEBp	M1fHXuKhWEKV	MXXpcoR2[2W\|IHTlcIF6\WRiYX7kJJN2e3SjaX7l[EBTV1NiaX7jdoVie2V?	Mke3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkO2NVMoRjJ2NEKzOlE{RC:jPh?=
CEM	NEWwRodHfW6ldHnvckBCe3OjeR?=	MlTKNlUxNzVyMH7N	M3LGTFQ5KGh?	MYpCpHBDWw>?	NWC1U\|VrcW6mdXPld{Bl\WyjeXXkJIFv\CC\|dYP0ZYlv\WRiUl;TJIlv[3KnYYPl	NH3Zfm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyN\|YyOyd-MkS0NlM3OTN:L3G+
HL-60	NX\rXZdsTnWwY4Tpc44hSXO\|YYm=	MorFNlUxNzVyMH7N	MoqxOFghcA>?	NXvnV\|ZsyqCSQmO=	NWPsWXU{cW6mdXPld{Bl\WyjeXXkJIFv\CC\|dYP0ZYlv\WRiUl;TJIlv[3KnYYPl	M1zne\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
ML-1	NY\xVZh3TnWwY4Tpc44hSXO\|YYm=	M{nkUlI2OC93MEDuUS=>	NVS1fXhOPDhiaB?=	M1\LT:KhWEKV	M{LSfolv\HWlZYOg[IVt[XmnZDDhcoQhe3W\|dHHpcoVlKFKRUzDpcoNz\WG\|ZR?=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
DLD-1	MYDGeY5kfGmxbjDBd5NigQ>?	M{HYdVI2OC93MEDuUS=>	M4e3cVQ5KGh?	NV3GVVJjyqCSQmO=	MYrkc{Bvd3RiaX7keYNmeyCmZXzhfYVlKGGwZDDzeZN1[WmwZXSgVm9UKGmwY4LlZZNm	M2TxRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
HCT-116	NIjnXmhHfW6ldHnvckBCe3OjeR?=	MVyyOVAwPTBybl2=	NVy0bHVPPDhiaB?=	MVlCpHBDWw>?	MkHP[I8hdm:2IHnu[JVk\XNiZHXsZZlm\CCjbnSgd5V{fGGrbnXkJHJQWyCrbnPy[YF{\Q>?	NWXRc41VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlM3OTNpPkK0OFI{PjF\|PD;hQi=>
U937-A/E-9/14/18	M1nEWGFxd3C2b4Ppd{BCe3OjeR?=	NED3bHYxNjBzL{CuNU8yNzFyIN88US=>	M13UN\|Q5KGh?		NWXsdJZOcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NY[wRotMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA1PTZpPkK0N\|AxPDV4PD;hQi=>
HT29	NXTH[oMxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M1;Ie\|czKGh?		NIrWN2NKSzVyPUG0NFDDuTF5OTFOwG0>	NHnvSGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3NlA3OSd-MkSxO\|IxPjF:L3G+
SW48	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MkLrO\|IhcA>?		NIrWUlVKSzVyPUG1MlLDuTZwMjFOwG0>	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5MkC2NUc,OjRzN{KwOlE9N2F-
HCT116	NGjr[VFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M3rleFczKGh?		MnvxTWM2OD1zLkhCtVAvPCEQvF2=	NIrCU3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3NlA3OSd-MkSxO\|IxPjF:L3G+
HepG2	MnKySpVv[3Srb36gRZN{[Xl?	M4fU[FAvPS9zIN88US=>	NWe3dWM{OjRiaB?=	MVPEUXNQ	M3zmVJVxNXKnZ4XsZZRm\CC2aHWgdoVt[XSrdnWgU2NVVjJibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:w	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2Nki3OEc,OjRzNE[4O\|Q9N2F-
LS174T	MnfWSpVv[3Srb36gRZN{[Xl?	M2ryPVAvPS9zIN88US=>	MV6yOEBp	NV3WNZV[TE2VTx?=	MWns[YFlKHSxIHHuJIlv[3KnYYPlJI9nKE:FVF6yJIxmfmWucx?=	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2Nki3OEc,OjRzNE[4O\|Q9N2F-
HepG2	MoX0RZBweHSxc3nzJGF{e2G7	NWLUXXdKOS9zMD:xNFAh\|ryP	M4G5R\|ch\A>?	M3PY[2ROW09?	NWDWTo5JcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	M1naN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS2PFc1Lz5{NEG0Olg4PDxxYU6=
LS174T	MoLORZBweHSxc3nzJGF{e2G7	M3nBNVEwOTBxMUCwJO69VQ>?	M1HoVlch\A>?	NXf1dVZVTE2VTx?=	MmSzbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>?	NXjUPYpTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFY5PzRpPkK0NVQ3QDd2PD;hQi=>
QBC-939	NF7mUmFCeG:ydH;zbZMhSXO\|YYm=	MW[xM\|ExNzFyMDFOwG0>	M{HxR\|ch\A>?	NGjudVVFVVOR	MXLpcoR2[2W\|IHPlcIwh[XCxcITvd4l{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz	NF7RWlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG0Olg4PCd-MkSxOFY5PzR:L3G+
U251	NUHtbGUxSXCxcITvd4l{KEG\|c3H5	NGe0dWgyNzFyL{GwNEDPxE1?	NFHiUWc4KGR?	M4nDdGROW09?	NGXlTnZqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2Nki3OEc,OjRzNE[4O\|Q9N2F-
HL-60	NVLiPVBVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mo\uNUDPxE1?	NFHk[Xc1QCCq		M1PrR4lv[3KnYYPld{BIOi2yaHHz[UBk\WyuIH\yZYN1cW:w	M2nKclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MECwN\|I1Lz5{NECwNFMzPDxxYU6=
MDA‑MB‑453	NHvzWnVHfW6ldHnvckBCe3OjeR?=	MnTMNE4zNzFizszN	NGjWNYo4OiCq		M4LDfYNifXOnczDy[U1mgHC{ZYPzbY9vKG:oIHPsZZVlcW8EoEJCpC=>	NFjPdGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OFIzQCd-MkO4OFQzOjh:L3G+
HCC1569	MXjGeY5kfGmxbjDBd5NigQ>?	NV3VV4FzOC5{L{Gg{txO	NWTJWW5bPzJiaB?=		Ml7vZ4F2e2W\|IILlMYV5eHKnc4Ppc44hd2ZiY3zheYRqdsLiMdMg	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NEKyPEc,OjN6NESyNlg9N2F-
BT‑474	NWTVWZlnTnWwY4Tpc44hSXO\|YYm=	Mk[3NE4zNzFizszN	MnvqO\|IhcA>?		NWrnbWx5[2G3c3XzJJJmNWW6cILld5Nqd25ib3[gZ4xifWSrbtMgNeKh	NFfsPXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OFIzQCd-MkO4OFQzOjh:L3G+
AGS	NFfPSlFCeG:ydH;zbZMhSXO\|YYm=	Mny0OU8yOC9{MD:1NEDPxE1?	M1rUe\|Q5yqCqwrC=	MnzkSG1UVw>?	NYPwe206cW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6yqB?	MnHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEK3PFQoRjJ\|NUiyO\|g1RC:jPh?=
A549	MoTHRZBweHSxc3nzJGF{e2G7	NHvxSGg2NzFyL{KwM\|UxKM7:TR?=	MXe0POKhcMLi	NFXq[\|hFVVOR	NH7tV\|lqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJnDqA>?	MmjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEK3PFQoRjJ\|NUiyO\|g1RC:jPh?=
AGS	M3jmWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYPUeotYPS9zMD:yNE82OCEQvF2=	NGLORok1QMLiaNMg	MXHEUXNQ	M1S2W4lv\HWlZYOgS\|IwVSCyaHHz[UBienKnc4VCpC=>	MorUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEK3PFQoRjJ\|NUiyO\|g1RC:jPh?=
Kasumi-1	M4DPfmFxd3C2b4Ppd{BCe3OjeR?=	MXmwMlUh\|ryP	MXS0POKhcMLi		NYfkOFNl\GWlcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClbz30doVifGWmIIfpeIghXGZvTmCtd4M>	MnzQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUOzOFgoRjJ\|NEmzN\|Q5RC:jPh?=
OCI-AML3	NEPDbldCeG:ydH;zbZMhSXO\|YYm=	NWXHU2wyOi53IN88US=>	Mlv6OFjDqGkEoB?=		NWjWeXJZ\GWlcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClbz30doVifGWmIIfpeIghXGZvTmCtd4M>	NFfVTGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5N\|M1QCd-MkO0PVM{PDh:L3G+
MV4-11	NGmzXmpCeG:ydH;zbZMhSXO\|YYm=	M2rySlIvPSEQvF2=	MW[0POKhcMLi		NFXzfYhl\WO{ZXHz[ZMhfGinIHPlcIwhfmmjYnnsbZR6KGOxLYTy[YF1\WRid3n0bEBV\i2QUD3zZy=>	M1r4fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEmzN\|Q5Lz5{M{S5N\|M1QDxxYU6=
NK	M3vVbGN6fG:2b4jpeJkhSXO\|YYm=	M1PiWFAvODJvMkCg{txO	NEHwR3I2KGR?		Mn3o[IVkemWjc3XzJJRp\SCleYTvcJl1cWNiYXP0bZZqfHlib3[gUmsh[2WubIOgZZQhcW62ZYLt[YRq[XSnIHPvcoNmdnS{YYTpc45{KHKnc4XseIlv\yCrbjDhJHUue2ijcHXkJIRwe2YkgKPy[ZNxd26\|ZTDjeZJ3\Q>?	NYDtb\|JIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNlgxQDhpPkKzN\|I5ODh6PD;hQi=>
NK	MVTBdI9xfG:\|aYOgRZN{[Xl?	MorSNE4xOi1{MDFOwG0>	NHnsRpM2KGR?		MmjL[IVkemWjc3WgUmsh[2WubDDwdo9tcW[ncnH0bY9vKGGwZDD2bYFjcWyrdImgZZMhfGinIHPvcoNmdnS{YYTpc44hcW6lcnXhd4Vl	NIr3S2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OyPFA5QCd-MkOzNlgxQDh:L3G+
NK	MWLGeY5kfGmxbjDBd5NigQ>?	NFH0eYwxNjBzLUKwJO69VQ>?	NYTndFBYPSCm		M1vVd4NifXOnczDofZBwdWW2aInsZZRqd25ib3[gUmsh[2WubIOgbY4h[SCmb4Pl5qCUemW\|cH;ud4U>	M{fST\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|M{K4NFg5Lz5{M{OyPFA5QDxxYU6=
MOLT4/DNR	NH\6ZoJHfW6ldHnvckBCe3OjeR?=	Mm\DOUDPxE1?	MVS0JIQ>		NUfYdWw{emWmdXPld{BCSkOEMTDtVm5CKGW6cILld5Nqd25?	M3ewc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[wOVcxLz5{M{C2NFU4ODxxYU6=
Jurkat/DOX	MonZSpVv[3Srb36gRZN{[Xl?	M3zaNVUh\|ryP	NFLofWQ1KGR?		NWjVbZB5emWmdXPld{BCSkOEMTDtVm5CKGW6cILld5Nqd25?	Mn;1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkC1O\|AoRjJ\|ME[wOVcxRC:jPh?=
MOLT4/DNR	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH;pVZI2KM7:TR?=	NWLDdnAzPCCm		NXLvS49yemWmdXPld{B1cGViSVO1NOKhfmGudXWg[o9zKGSjdX7vdpVjcWOrbjDz[Y5{cXSrdnn0fS=>	NWK4UY9IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
Jurkat/DOX	M{LVZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4LTTFUh\|ryP	MnT0OEBl		M4HrdJJm\HWlZYOgeIhmKEmFNUFCpJZidHWnIH\vdkBl[XWwb4L1ZolkcW5ic3Xud4l1cX[rdIm=	NUj0UZBLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
ccRCC	NW\ISZk4SXCxcITvd4l{KEG\|c3H5	M2nFXFAvODFvMUFOwG0>	NG\1cGQ4OiCq	MYrEUXNQ	NYfWc4dQcGG\|IH3pcolu[WxiZX\m[YN1KG:wIHPlcIwheHKxbHnm[ZJifGmxbh?=	NGLWN4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOlQ3Pyd-MkK4NlY1Pjd:L3G+
TNBC	MUDBdI9xfG:\|aYOgRZN{[Xl?	NHOxNm0xNjBzLUGw{txO	NGDsOmU4OiCq	NWrQb4s4TE2VTx?=	MYXoZZMhdWmwaX3hcEBm\m[nY4Sgc44h[2WubDDwdo9tcW[ncnH0bY9v	NW\0bmZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
A498	NV[4elhiSXCxcITvd4l{KEG\|c3H5	Mlv2NE4xOS1zMN88US=>	MoTLO\|IhcA>?	NFHKN2FFVVOR	NIPOeIFqdmS3Y3XzJJN6dmW{Z3nzeIlkKHKnc4DvcpNme8Lid3n0bEBzd22rZHXwd4lv	MmXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Mk[0OlcoRjJ{OEK2OFY4RC:jPh?=
KIJ265T	NFvIRo1CeG:ydH;zbZMhSXO\|YYm=	NH3zWGcxNjBzLUGw{txO	MVG3NkBp	NXWxN3RDTE2VTx?=	MYPpcoR2[2W\|IIP5coVz\2m\|dHnjJJJme3CxboPld:Khf2m2aDDyc41q\GWyc3nu	NUDYcJF5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
MDA-231	NInxZmNCeG:ydH;zbZMhSXO\|YYm=	M321dVAvODFvMUFOwG0>	NVvUfYRJPzJiaB?=	MmDISG1UVw>?	MWnpcoR2[2W\|IIP5coVz\2m\|dHnjJJJme3CxboPld:Khf2m2aDDyc41q\GWyc3nu	NYX0[ZZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
BT-20	NX\lfoNsSXCxcITvd4l{KEG\|c3H5	MUewMlAyNTFyzszN	MYq3NkBp	M2LDbmROW09?	MmHnbY5lfWOnczDzfY5memerc4TpZ{Bz\XOyb37z[ZPDqHerdHigdo9ucWSncIPpci=>	M{GzdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK2OFY4Lz5{MkiyOlQ3PzxxYU6=
U937	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXfGXY5RPS1{MDFOwG0>	M2XPSlI1NzR6L{eyJIg>		NXPEXFBjcW6mdXPld{BiKGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd4N{CyNUc,OjJ5NkewNlE9N2F-
HL60	M1zZZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MUe1MVIxKM7:TR?=	MVGyOE81QC95MjDo		M2fKR4lv\HWlZYOgZUBl\WO{ZXHz[UBqdiClZXzsJJZq[WKrbHn0fUBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI>	MmXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NkewNlEoRjJ{N{[3NFIyRC:jPh?=
U937	NFLGfnBCeG:ydH;zbZMhSXO\|YYm=	MmfuNVUh\|ryP	M2ThcFI1NzR6L{eyJIg>		MmjxbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	NIr1[2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke2O\|AzOSd-MkK3OlcxOjF:L3G+
HL60	NWPVTXJnSXCxcITvd4l{KEG\|c3H5	NXrQcm5sOTVizszN	MYqyOE81QC95MjDo		M4[we4lv\HWlZYOgZ4VtdCCjcH;weI9{cXN?	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd4N{CyNUc,OjJ5NkewNlE9N2F-
LS411N	Ml\mRZBweHSxc3nzJGF{e2G7	NF3Te2YxNjVizszN	Mke0O\|IhcA>?		MmTQbY5kemWjc3XzJGZieyCvUl7BJIxmfmWu	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4MU[5OUc,OjJ2NkG2PVU9N2F-
MDA-MB-231	NWjsfoVmSXCxcITvd4l{KEG\|c3H5	NETINZgyOCEQvF2=	MYe0PEBp		NEPQWXpz\WS3Y3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	M{DlXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEi3Olk4Lz5{MUi4O\|Y6PzxxYU6=
MCF-7	M4LzU2Fxd3C2b4Ppd{BCe3OjeR?=	MnnQNVAh\|ryP	M3iwRlQ5KGh?		NFrESI5z\WS3Y3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	NVjoN3k1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4PFc3QTdpPkKxPFg4Pjl5PD;hQi=>
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Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Survivin / Bcl-2 / p53 / c-Myc / DNMT1 ; p-AKT / AKT / p-GSK3β / GSK3β / p-Myc / Myc / p-P70 / P70 / p-4EBP-1 / 4EBP-1 / PTEN ; phospho-p38 / p38 / phospho-NFκB / NFκB ; p-JAK1 / JAK1 / p-JAK2 / JAK2 / p-STAT3 / STAT3 ; E-cadherin / N-cadherin / Snail / MMP-2 / MMP-9 / Bcl-2 / Bax	26384351 25762617 26617834 26692933 28152502
Immunofluorescence	DNMT1 ; E-cadherin / MMP-9	21303982 28152502
Growth inhibition assay	Cell viability	26384351

In vivo

In a ALK+ KARPAS-299 murine xenograft model, Decitabine at a dose of 2.5 mg/kg causes increased apoptosis and reduced proliferation of tumor cells, and also results in demethylation of tumor suppressor p16INK4A. ^[2]

Protocol (from reference)

Kinase Assay:^[1]	DNA synthesis assay : The rate of DNA synthesis is measured by the incorporation of radioactive thymidine into DNA. HL-60 and KG1a cells are suspended in 2 mL RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 hours (drugs are added simultaneously). At 48 hours, 0.5 μCi [³H] thymidine (6.7 Ci/mmol) is added to each well and incubated for an additional 24 hours. The cells are placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA are then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using Beckman LS 6000IC scintillation counter. The IC50 is defined as the concentration of drug that inhibits 50% of the DNA synthesis of the leukemic cell lines from the dose–response curve.
Cell Research:^[1]	Cell lines: HL-60 and KG1a Concentrations: 0-500 nM Incubation Time: 96 hours Method: For the growth inhibition assay, cells in log phase are placed in 5 mL of medium. Different concentrations of Decitabine are added to the medium simultaneously. Cell counts are performed at the indicated times using a model ZM Coulter Counter. The concentration that produces 50% inhibition of growth (IC50) is determined from the growth curves of the drug treated leukemic cell lines. (Only for Reference)
Animal Research:^[2]	Animal Models: KARPAS-299 human cells are inoculated subcutaneously into the right and left flanks of the mice. Dosages: ≤2.5 mg/kg Administration: Administered via i.p. (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	45 mg/mL (197.18 mM)
	Water	10 mg/mL warmed (43.81 mM)
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): water For best results, use promptly after mixing.	10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	228.21
Formula	C₈H₁₂N₄O₄
CAS No.	2353-33-5
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1C(C(OC1N2C=NC(=NC2=O)N)CO)O

Download Decitabine (NSC 127716) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04985656	Withdrawn	Drug: Pevonedistat\|Drug: Decitabine\|Drug: Cedazuridine	Myelodysplastic Syndromes (MDS)	Takeda	October 1 2021	Phase 2
NCT04854889	Recruiting	Drug: Decitabine	Refractory Aplastic Anemia	Institute of Hematology & Blood Diseases Hospital	April 22 2021	Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is S1200 a racemic mixture or a monomer?

Answer:
S1200 is R form

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