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Decitabine (NSC 127716)

Name: Decitabine (NSC 127716)
Brand: Selleck Chemicals
SKU: S1200
Rating: 5 (138 reviews)

Catalog No.S1200 Synonyms: Deoxycytidine, Dacogen, 5-aza-2'-deoxycytidine, 5-AZA-dC, 5-aza-CdR,NSC 127716

For research use only.

Decitabine (NSC 127716, Deoxycytidine, Dacogen, 5-aza-2-deoxycytidine, 5-AZA-dC, 5-aza-CdR) is a DNA methyltransferase inhibitor, incorporating into DNA and resulting in hypomethylation of DNA and intra-S-phase arrest of DNA replication. It is used to treat myelodysplastic syndrome (MDS). Decitabine induces cell cycle arrest and apoptosis in various cancer cell lines.

Decitabine (NSC 127716) Chemical Structure

CAS No. 2353-33-5

Selleck's Decitabine (NSC 127716) has been cited by 138 publications

Purity & Quality Control

Choose Selective DNA Methyltransferase Inhibitors

Related Compound Libraries

Biological Activity

Description

Targets

DNA methylation ^[1]
(HL-60, KG1a cells)

In vitro

Decitabine inhibits cell growth in a dose and time-dependent manner with IC50 of approximately 438 nM and 43.8 nM for 72 hours and 96 hours exposure in HL-60 and KG1a leukemic cells, respectively. ^[1] A recent study shows that Decitabine exhibits high anti-proliferative and pro-apoptotic activity against anaplastic large cell lymphoma (ALCL), and inhibits [³H]–thymidine uptake in KARPAS-299 cells with EC50 of 0.49 μM. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Eca109	M4HTfmZ2dmO2aX;uJGF{e2G7	NUDCU4VkOC53wrFOwG0>	MV6yOEBp	MXr3ZZRmeg>?	Mom1cY9lfWyjdHXzJJRp\SCpZX7lJIV5eHKnc4Ppc44hd2ZiTVHHSU1CKG2nbXLldpM>	NFzpS209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN\|A5Oid-MkWxNlMxQDJ:L3G+
Eca109	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NE\RbXYxNjVxMj61M\|Uh\|ryP	Mnz3NlQwPDhxN{KgbC=>	NITBS5p4[XSnch?=	Ml3CbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEBld3OnLTDhcoQhfGmvZT2g[IVx\W6mZX70JI1idm6nch?=	MlPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
Eca109	NELre3dHfW6ldHnvckBCe3OjeR?=	NV;3Z2ZyOC53wrFOwG0>	NH7lTIM3NzF{L{K0JIg>	MoK1e4F1\XJ?	MkL2bY5pcWKrdIOgZ4VtdCCvaXfyZZRqd28EoB?=	NUDOVGdNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlMxQDJpPkK1NVI{ODh{PD;hQi=>
Eca109	M1;4e2Z2dmO2aX;uJGF{e2G7	NXjmbmdkOC53wrFOwG0>	M{LLPVI1KGh?	NYjhb\|VLf2G2ZYK=	M3;jcIlvcGmkaYTzJINmdGxiaX72ZZNqd25?	NGjsfmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN\|A5Oid-MkWxNlMxQDJ:L3G+
Eca109	NHLtUFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVTaWYU3OC53wrFOwG0>	M3njV\|I1KGh?	MYD3ZZRmeg>?	MXTpcoR2[2W\|IFeyM20h[XK{ZYP0JIlvKHSqZTDj[YxtKGO7Y3zl	NFHUR3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyN\|A5Oid-MkWxNlMxQDJ:L3G+
Eca109	M1rXT2Z2dmO2aX;uJGF{e2G7	MYCwMlUwOSEQvF2=	MXmyOEBp	NX\JfpY6f2G2ZYK=	MYnk[YNz\WG\|ZYOg[ZhxemW\|c3nvckBw\iCQRj5OvmIzKGGwZDDNUXAz	MmnuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkOwPFIoRjJ3MUKzNFgzRC:jPh?=
SW1116	MkPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYLUPZM5OC53L{GvNk82KM7:TR?=	MnPIOFghcA>?	MYrEUXNQ	MXTlcohidmOnczD0bIUhT2WoaYTpcoljKGmwZIXj[YQh[2WubDDpcohq[mm2aX;u	MlrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6N{SyPFYoRjJ2OEe0Nlg3RC:jPh?=
LOVO	M2rseWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWOwMlUwOS9{L{Wg{txO	M2fXcVQ5KGh?	NFP0U5hFVVOR	NVrSPYQ6\W6qYX7j[ZMhfGinIFfl[ol1cW6rYjDpcoR2[2WmIHPlcIwhcW6qaXLpeIlwdg>?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5NEK4Okc,OjR6N{SyPFY9N2F-
SW1116	NVzkRYlkTnWwY4Tpc44hSXO\|YYm=	MYmxNEDPxE1?	MXS0PEBp	M{nCR2ROW09?	MUjpcoNz\WG\|ZYOgeIhmKGWoZnXjeIl3\SCjdDDpcohq[mm2aX7nJGFMXCCjbnSgcXRQWiC\|aXfuZYxqdmdicHH0bJdigXNiY3;tZolv\WRid3n0bEBo\W[rdHnubYI>	M4PhdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
LOVO	MVfGeY5kfGmxbjDBd5NigQ>?	NFL4[FQyOCEQvF2=	M13K[FQ5KGh?	NF\IOFFFVVOR	M1Lk[Ilv[3KnYYPld{B1cGViZX\m[YN1cX[nIHH0JIlvcGmkaYTpcochSUuWIHHu[EBuXE:UIIPp[45idGmwZzDwZZRpf2G7czDjc41jcW6nZDD3bZRpKGenZnn0bY5q[g>?	M{DlcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEe0Nlg3Lz5{NEi3OFI5PjxxYU6=
SW1116	NE[zUHlCeG:ydH;zbZMhSXO\|YYm=	NW\RRpl2OTBizszN	M2G4N\|Q5KGh?	NXPiPHd[TE2VTx?=	NXTZS3Bx\W6qYX7j[ZMhT2WoaYTpcoljNWmwZIXj[YQh[XCxcITvd4l{	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh5NEK4Okc,OjR6N{SyPFY9N2F-
LOVO	M4LoemFxd3C2b4Ppd{BCe3OjeR?=	M{nme\|ExKM7:TR?=	Mmr2OFghcA>?	MnvJSG1UVw>?	MXvlcohidmOnczDH[YZqfGmwaXKtbY5lfWOnZDDhdI9xfG:\|aYO=	NGrIR2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3OFI5Pid-MkS4O\|QzQDZ:L3G+
RPMI-8226	M2PlZ2Fxd3C2b4Ppd{BCe3OjeR?=	NXzodJh{OS9{IN88US=>	MnfsOFgwPzJxOU[gbC=>	MV7EUXNQ	NEnVWW5qdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	M{HYc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
OPM-2	NYjXNZBmSXCxcITvd4l{KEG\|c3H5	M{DmRlEwOiEQvF2=	MVu3Nk86Pi9zMkCgbC=>	MXTEUXNQ	MYfpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	NYjkPHFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4N\|MyODhpPkK0PFM{OTB6PD;hQi=>
JJN3	NUjmPHJvSXCxcITvd4l{KEG\|c3H5	NW\tNINJOC53L{Gg{txO	NGHVPI0zPC92ODDo	Mk[1SG1UVw>?	MWnpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	M4jw[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
NCI-H929	NFP6RVlCeG:ydH;zbZMhSXO\|YYm=	NFrPVlcyNzJizszN	NHzJeGE4Oi97Nj:xNlAhcA>?	NX36UWtRTE2VTx?=	MUDpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	M4fhZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
RPMI-8226	NG\NOohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3rKNVEwOiEQvF2=	NV7TNFY{OjRxNEivO\|IhcA>?	NU[wT3VzTE2VTx?=	MorCZYZn\WO2czDj[YxtKGO7Y3zlJJBzd2e{ZYPzbY9vKG6nZ3H0bZZmdHl?	NILFXJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEizN\|ExQCd-MkS4N\|MyODh:L3G+
OPM-2	MnizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2DBc\|EwOiEQvF2=	Mk\UNlQwPDhxN{KgbC=>	M1PhSmROW09?	Mk\0ZYZn\WO2czDj[YxtKGO7Y3zlJJBzd2e{ZYPzbY9vKG6nZ3H0bZZmdHl?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh\|M{GwPEc,OjR6M{OxNFg9N2F-
JJN3	MnvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NEXiVIgxNjVxMTFOwG0>	M4TTelI1NzR6L{eyJIg>	NXXtSIZjTE2VTx?=	MXnh[oZm[3S\|IHPlcIwh[3mlbHWgdJJw\3Knc4Ppc44hdmWpYYTpeoVtgQ>?	M3HYfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEOzNVA5Lz5{NEizN\|ExQDxxYU6=
NCI-H929	NFr4RpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHuwU3kyNzJizszN	NWDLVHJQOjRxNEivO\|IhcA>?	MV;EUXNQ	NXfCeWtz[W[oZXP0d{Bk\WyuIHP5Z4xmKHC{b3fy[ZN{cW:wIH7l[4F1cX[nbIm=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh\|M{GwPEc,OjR6M{OxNFg9N2F-
HeLa	M{TqT2tqdmG\|ZTDBd5NigQ>?				M1nFeWtqRTFyMEFihLM2ODByIN88UUBnd3JiaFXOWFE>	NYHlVZlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PFAxQThpPkK0O\|gxODl6PD;hQi=>
HeLa	NF[4O5ZMcW6jc3WgRZN{[Xl?				NHH2fFBMcT13Lk[gxtEhOC53IN88UUBnd3JiaFXOWFI>	NXHEfW1JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PFAxQThpPkK0O\|gxODl6PD;hQi=>
HeLa	MoHCT4lv[XOnIFHzd4F6				NHnUfHNMcT1{MT62JOKyKDNwMDFOwG0h\m:{IHjDUnQy	M3\USlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{iwNFk5Lz5{NEe4NFA6QDxxYU6=
HeLa	M1r0emtqdmG\|ZTDBd5NigQ>?				NX\t[pZzU2l;MUSuOEDDuSB2Lk[g{txOKG[xcjDoR25VOw>?	NVywR3d7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3PFAxQThpPkK0O\|gxODl6PD;hQi=>
NB4	M3\kXWZ2dmO2aX;uJGF{e2G7	MVSyMlUwPS95LkWvNVAh\|ryP	MV6yOEBp	M{jZdmROW09?	M13Rb4lv[3KnYYPlZUB1cGViZYjwdoV{e2mxbjDv[kBxemWldYLzc5IhdWmULUGyOYE>	M1PyclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEi0PFcxLz5{NES4OFg4ODxxYU6=
CD4+ CD25− T	M4HnWWZ2dmO2aX;uJGF{e2G7	NVT2eHBpOS93IN88US=>			Mm\ydoVlfWOnUzDncI9j[WxiRF7BJI1mfGi7bHH0bY9v	Mn3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{[zOlAoRjJ2NEe2N\|YxRC:jPh?=
BV-173	M2HDcGFxd3C2b4Ppd{BCe3OjeR?=	NVPYeoJ5OC5{NT:wMlUwOC55NT:xJO69VQ>?	M2T3RVQ5Nzd{L{m2JIg>	MVdCpHBDWw>?	MlLxbY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDic5RpKGSxc3WtJIFv\CC2aX3lMUBl\XCnbnTlcpQhdWGwbnXy	NYPsbFJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NlM3OTNpPkK0OFI{PjF\|PD;hQi=>
ML-1	NHTnTJlCeG:ydH;zbZMhSXO\|YYm=	NUnO[lc6OC5{NT:wMlUwOC55NT:xJO69VQ>?	MmfPOFgwPzJxOU[gbC=>	NXeyNYZGyqCSQmO=	NHG3U5pqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI>	MoOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkO2NVMoRjJ2NEKzOlE{RC:jPh?=
HL-60	MXTBdI9xfG:\|aYOgRZN{[Xl?	M1vVcVAvOjVxMD61M\|AvPzVxMTFOwG0>	NGPtdWU1QC95Mj:5OkBp	M3rTSeKhWEKV	NF[wU\|lqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnuJIJwfGhiZH;z[U0h[W6mIITpcYUuKGSncHXu[IVvfCCvYX7u[ZI>	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
KG-1a	MofURZBweHSxc3nzJGF{e2G7	MV2wMlI2NzBwNT:wMlc2NzFizszN	MYG0PE84Oi97NjDo	Mn3sxsBRSlN?	M17TSIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZo91cCCmb4PlMUBidmRidHnt[U0h\GWyZX7k[Y51KG2jbn7ldi=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
BV-173	MU\GeY5kfGmxbjDBd5NigQ>?	NGrkfHQzPTBxNUCwcm0>	M3viZ\|Q5KGh?	MlLhxsBRSlN?	NIG1WYRqdmS3Y3XzJIRmdGG7ZXSgZY5lKHO3c4ThbY5m\CCUT2OgbY5kemWjc3W=	M3O1PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
CEM	NXrrOYY2TnWwY4Tpc44hSXO\|YYm=	NFfYd5YzPTBxNUCwcm0>	M3myTFQ5KGh?	MYZCpHBDWw>?	MoXkbY5lfWOnczDk[YxigWWmIHHu[EB{fXO2YXnu[YQhWk:VIHnuZ5Jm[XOn	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HL-60	M1mxU2Z2dmO2aX;uJGF{e2G7	MlS1NlUxNzVyMH7N	NYfHZYRpPDhiaB?=	M33hfuKhWEKV	NXj2WZVncW6mdXPld{Bl\WyjeXXkJIFv\CC\|dYP0ZYlv\WRiUl;TJIlv[3KnYYPl	M2jFNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEKzOlE{Lz5{NESyN\|YyOzxxYU6=
ML-1	NHv6eXRHfW6ldHnvckBCe3OjeR?=	NXPtPY56OjVyL{WwNI5O	NETjT2c1QCCq	NYLlbYx[yqCSQmO=	M4HmfYlv\HWlZYOg[IVt[XmnZDDhcoQhe3W\|dHHpcoVlKFKRUzDpcoNz\WG\|ZR?=	MmDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkO2NVMoRjJ2NEKzOlE{RC:jPh?=
DLD-1	NVfOUG1STnWwY4Tpc44hSXO\|YYm=	MoPnNlUxNzVyMH7N	M3HlOVQ5KGh?	M1LZSOKhWEKV	NYPZ[lBx\G9ibn;0JIlv\HWlZYOg[IVt[XmnZDDhcoQhe3W\|dHHpcoVlKFKRUzDpcoNz\WG\|ZR?=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR{M{[xN{c,OjR2MkO2NVM9N2F-
HCT-116	MkX0SpVv[3Srb36gRZN{[Xl?	MUiyOVAwPTBybl2=	NVXndVNTPDhiaB?=	NUnhWGE3yqCSQmO=	MnXt[I8hdm:2IHnu[JVk\XNiZHXsZZlm\CCjbnSgd5V{fGGrbnXkJHJQWyCrbnPy[YF{\Q>?	NFXFc3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESyN\|YyOyd-MkS0NlM3OTN:L3G+
U937-A/E-9/14/18	M2POZmFxd3C2b4Ppd{BCe3OjeR?=	NUThSGpzOC5yMT:wMlEwOS9zMDFOwG0>	MkjnOFghcA>?		NX3LTJhZcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NXzqS2V3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNFA1PTZpPkK0N\|AxPDV4PD;hQi=>
HT29	NV[2WHlDT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NYOyc2QxPzJiaB?=		MWPJR\|UxRTF2MEFCtVE4QSEQvF2=	NXHXVY5HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO\|IxPjFpPkK0NVczODZzPD;hQi=>
SW48	MlTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		Mm\5O\|IhcA>?		MnjFTWM2OD1zNT6yxtE3NjJizszN	NEnaNFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3NlA3OSd-MkSxO\|IxPjF:L3G+
HCT116	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NXrxXHhCPzJiaB?=		NFjRTm1KSzVyPUGuO:KyOC52IN88US=>	M4PYcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUeyNFYyLz5{NEG3NlA3OTxxYU6=
HepG2	MUTGeY5kfGmxbjDBd5NigQ>?	NUnkNnpUOC53L{Gg{txO	MY[yOEBp	MWjEUXNQ	M{Th[JVxNXKnZ4XsZZRm\CC2aHWgdoVt[XSrdnWgU2NVVjJibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:w	NWnxflFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFY5PzRpPkK0NVQ3QDd2PD;hQi=>
LS174T	MXXGeY5kfGmxbjDBd5NigQ>?	MoO4NE42NzFizszN	NXftTpc6OjRiaB?=	NX3DeGhtTE2VTx?=	NWGxZogxdGWjZDD0c{BidiCrbnPy[YF{\SCxZjDPR3RPOiCuZY\lcJM>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF2Nki3OEc,OjRzNE[4O\|Q9N2F-
HepG2	NH\WR5VCeG:ydH;zbZMhSXO\|YYm=	NInIO2syNzFyL{GwNEDPxE1?	NGPUR2k4KGR?	M4fzUGROW09?	MoXobY5lfWOnczDj[YxtKGGyb4D0c5NqeyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>?	MmW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNE[4O\|QoRjJ2MUS2PFc1RC:jPh?=
LS174T	NH7CV2hCeG:ydH;zbZMhSXO\|YYm=	NYP5O4RMOS9zMD:xNFAh\|ryP	NHTydXM4KGR?	NFXPNHNFVVOR	NXPOTldvcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NXvvXnlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFY5PzRpPkK0NVQ3QDd2PD;hQi=>
QBC-939	M2ixN2Fxd3C2b4Ppd{BCe3OjeR?=	MWixM\|ExNzFyMDFOwG0>	NF\WV4s4KGR?	NGHhe\|NFVVOR	M{TsZYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ?	NUDITFM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOFY5PzRpPkK0NVQ3QDd2PD;hQi=>
U251	MnTNRZBweHSxc3nzJGF{e2G7	MV2xM\|ExNzFyMDFOwG0>	MmPzO{Bl	M1q4NmROW09?	NIj5R\|FqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	M{nnbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUS2PFc1Lz5{NEG0Olg4PDxxYU6=
HL-60	MoDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2m2XlEh\|ryP	MlHrOFghcA>?		M2HMc4lv[3KnYYPld{BIOi2yaHHz[UBk\WyuIH\yZYN1cW:w	NU\ZfGtyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNFA{OjRpPkK0NFAxOzJ2PD;hQi=>
MDA‑MB‑453	MmXLSpVv[3Srb36gRZN{[Xl?	NFHiZYQxNjJxMTFOwG0>	NVLnS\|FLPzJiaB?=		NFH0[2lk[XW\|ZYOgdoUu\XiycnXzd4lwdiCxZjDjcIF2\GmwwrCxxsA>	MmrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NESyNlgoRjJ\|OES0NlI5RC:jPh?=
HCC1569	NV\TXnU4TnWwY4Tpc44hSXO\|YYm=	MmrBNE4zNzFizszN	NHXmW444OiCq		NH\aVoFk[XW\|ZYOgdoUu\XiycnXzd4lwdiCxZjDjcIF2\GmwwrCxxsA>	NW\LZ5Q5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFQzOjhpPkKzPFQ1OjJ6PD;hQi=>
BT‑474	MYXGeY5kfGmxbjDBd5NigQ>?	NIjwcVkxNjJxMTFOwG0>	NYTuS4ZDPzJiaB?=		NFqze2Vk[XW\|ZYOgdoUu\XiycnXzd4lwdiCxZjDjcIF2\GmwwrCxxsA>	M2Xib\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OES0NlI5Lz5{M{i0OFIzQDxxYU6=
AGS	M3riXWFxd3C2b4Ppd{BCe3OjeR?=	NG\6UmY2NzFyL{KwM\|UxKM7:TR?=	MWe0POKhcMLi	NYXuVHZuTE2VTx?=	NEOzPIlqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJnDqA>?	NV3INlRuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PFI4QDRpPkKzOVgzPzh2PD;hQi=>
A549	M2XpNmFxd3C2b4Ppd{BCe3OjeR?=	MnTUOU8yOC9{MD:1NEDPxE1?	MoT2OFjDqGkEoB?=	MnPISG1UVw>?	MWTpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdIpCpC=>	M{D5SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUiyO\|g1Lz5{M{W4Nlc5PDxxYU6=
AGS	M4fMU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MV[1M\|ExNzJyL{WwJO69VQ>?	MmDpOFjDqGkEoB?=	M33OcGROW09?	MnHqbY5lfWOnczDHNk9OKHCqYYPlJIFzemW\|dNMg	MnXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OEK3PFQoRjJ\|NUiyO\|g1RC:jPh?=
Kasumi-1	M3HGSWFxd3C2b4Ppd{BCe3OjeR?=	M2fGXlAvPSEQvF2=	MnfoOFjDqGkEoB?=		NWPyXHox\GWlcnXhd4V{KHSqZTDj[YxtKH[rYXLpcIl1gSClbz30doVifGWmIIfpeIghXGZvTmCtd4M>	MnjyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUOzOFgoRjJ\|NEmzN\|Q5RC:jPh?=
OCI-AML3	NVXDcYZLSXCxcITvd4l{KEG\|c3H5	NHfifFMzNjVizszN	NHH3N4o1QMLiaNMg		MV7k[YNz\WG\|ZYOgeIhmKGOnbHygeoli[mmuaYT5JINwNXS{ZXH0[YQhf2m2aDDU[k1PWC2\|Yx?=	M3:0XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEmzN\|Q5Lz5{M{S5N\|M1QDxxYU6=
MV4-11	MV7BdI9xfG:\|aYOgRZN{[Xl?	M2jSZVIvPSEQvF2=	M1:4WVQ5yqCqwrC=		MU\k[YNz\WG\|ZYOgeIhmKGOnbHygeoli[mmuaYT5JINwNXS{ZXH0[YQhf2m2aDDU[k1PWC2\|Yx?=	NUTaV5ViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM{PDhpPkKzOFk{OzR6PD;hQi=>
NK	MkjSR5l1d3SxeHn0fUBCe3OjeR?=	MVywMlAzNTJyIN88US=>	MX21JIQ>		NX7vUGZw\GWlcnXhd4V{KHSqZTDjfZRwdHm2aXOgZYN1cX[rdImgc4YhVktiY3XscJMh[XRiaX70[ZJu\WSrYYTlJINwdmOnboTyZZRqd26\|IILld5VtfGmwZzDpckBiKFVvc3jhdIVlKGSxc3ZihLNz\XOyb37z[UBkfXK4ZR?=	NYq2SnpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNlgxQDhpPkKzN\|I5ODh6PD;hQi=>
NK	NXPzVI43SXCxcITvd4l{KEG\|c3H5	NGXWOnYxNjB{LUKwJO69VQ>?	NFSzUGE2KGR?		NIL2cW9l\WO{ZXHz[UBPUyClZXzsJJBzd2yrZnXyZZRqd25iYX7kJJZq[WKrbHn0fUBieyC2aHWgZ49v[2WwdILheIlwdiCrbnPy[YF{\WR?	Mk\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|MkiwPFgoRjJ\|M{K4NFg5RC:jPh?=
NK	M2nmNGZ2dmO2aX;uJGF{e2G7	MlzFNE4xOS1{MDFOwG0>	M3zSOlUh\A>?		M3\ZcINifXOnczDofZBwdWW2aInsZZRqd25ib3[gUmsh[2WubIOgbY4h[SCmb4Pl5qCUemW\|cH;ud4U>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{OEC4PEc,OjN\|MkiwPFg9N2F-
MOLT4/DNR	MkPKSpVv[3Srb36gRZN{[Xl?	M2Xnb\|Uh\|ryP	MknROEBl		MorVdoVlfWOnczDBRmNDOSCvUl7BJIV5eHKnc4Ppc44>	NWHUe\|BmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlA2PzBpPkKzNFYxPTdyPD;hQi=>
Jurkat/DOX	M1jvfGZ2dmO2aX;uJGF{e2G7	MlfWOUDPxE1?	NVLmS3k5PCCm		NYqxfXJ5emWmdXPld{BCSkOEMTDtVm5CKGW6cILld5Nqd25?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MEW3NEc,OjNyNkC1O\|A9N2F-
MOLT4/DNR	M{LhS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mm\pOUDPxE1?	NFvsOHU1KGR?		MlixdoVlfWOnczD0bIUhUUN3MNMgeoFtfWViZn;yJIRifW6xcoXibYNqdiC\|ZX7zbZRqfmm2eR?=	NIrVR2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NFU4OCd-MkOwOlA2PzB:L3G+
Jurkat/DOX	NYDvSHZMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoXaOUDPxE1?	MUe0JIQ>		NWjqfnk5emWmdXPld{B1cGViSVO1NOKhfmGudXWg[o9zKGSjdX7vdpVjcWOrbjDz[Y5{cXSrdnn0fS=>	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MEW3NEc,OjNyNkC1O\|A9N2F-
ccRCC	MkPkRZBweHSxc3nzJGF{e2G7	NFHpbYMxNjBzLUGw{txO	MkDkO\|IhcA>?	MlPqSG1UVw>?	MoDUbIF{KG2rbnntZYwh\W[oZXP0JI9vKGOnbHygdJJwdGmoZYLheIlwdg>?	MoDzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Mk[0OlcoRjJ{OEK2OFY4RC:jPh?=
TNBC	Mn7rRZBweHSxc3nzJGF{e2G7	NHW0XHUxNjBzLUGw{txO	MlrsO\|IhcA>?	NIXB[VRFVVOR	NInZPZRp[XNibXnubY1idCCnZn\lZ5Qhd25iY3XscEBxem:uaX\ldoF1cW:w	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
A498	NF;1NXdCeG:ydH;zbZMhSXO\|YYm=	NWe2VIpXOC5yMT2xNO69VQ>?	NVXMVZJqPzJiaB?=	NVWwTI9bTE2VTx?=	NVvxUmZXcW6mdXPld{B{gW6ncnfpd5Rq[yC{ZYPwc45{\XQEoIfpeIghem:vaXTldJNqdg>?	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{NkS2O{c,OjJ6Mk[0Olc9N2F-
KIJ265T	Ml3GRZBweHSxc3nzJGF{e2G7	MlvhNE4xOS1zMN88US=>	NUP3NVhvPzJiaB?=	NX7KNmF6TE2VTx?=	NVHlPY13cW6mdXPld{B{gW6ncnfpd5Rq[yC{ZYPwc45{\XQEoIfpeIghem:vaXTldJNqdg>?	Ml;NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6Mk[0OlcoRjJ{OEK2OFY4RC:jPh?=
MDA-231	NEWzUWlCeG:ydH;zbZMhSXO\|YYm=	MVmwMlAyNTFyzszN	MmP1O\|IhcA>?	MkjMSG1UVw>?	MnfBbY5lfWOnczDzfY5memerc4TpZ{Bz\XOyb37z[ZPDqHerdHigdo9ucWSncIPpci=>	NUDLN4dXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
BT-20	NF;YVm9CeG:ydH;zbZMhSXO\|YYm=	M1viOFAvODFvMUFOwG0>	MmizO\|IhcA>?	MXHEUXNQ	NGi0S2NqdmS3Y3XzJJN6dmW{Z3nzeIlkKHKnc4DvcpNme8Lid3n0bEBzd22rZHXwd4lv	NWXlcnN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlY1PjdpPkKyPFI3PDZ5PD;hQi=>
U937	NVe0NnZYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Ml\QOU0zOCEQvF2=	M1;DdFI1NzR6L{eyJIg>		MVrpcoR2[2W\|IHGg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgbY4h[SClb37j[Y51emG2aX;uMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy	M4DB[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{[3NFIyLz5{Mke2O\|AzOTxxYU6=
HL60	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NHjxTY82NTJyIN88US=>	NEm3S\|QzPC92OD:3NkBp		NX\H[HVOcW6mdXPld{BiKGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	Mk\TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NkewNlEoRjJ{N{[3NFIyRC:jPh?=
U937	MlXpRZBweHSxc3nzJGF{e2G7	MnmxNVUh\|ryP	MWeyOE81QC95MjDo		NHuzPW1qdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd4N{CyNUc,OjJ5NkewNlE9N2F-
HL60	Mn\CRZBweHSxc3nzJGF{e2G7	NH31R\|cyPSEQvF2=	NIPLS3EzPC92OD:3NkBp		NV\XNIxMcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	NXjFTnlzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OlcxOjFpPkKyO\|Y4ODJzPD;hQi=>
LS411N	MXTBdI9xfG:\|aYOgRZN{[Xl?	Mn7SNE42KM7:TR?=	MYS3NkBp		M2LMWIlv[3KnYYPld{BH[XNibWLORUBt\X[nbB?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4MU[5OUc,OjJ2NkG2PVU9N2F-
MDA-MB-231	NIruUJVCeG:ydH;zbZMhSXO\|YYm=	NVHXPIJ7OTBizszN	MWS0PEBp		MXLy[YR2[2W\|IHPlcIwhfmmjYnnsbZR6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz	NEjWTIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi4O\|Y6Pyd-MkG4PFc3QTd:L3G+
MCF-7	MlPmRZBweHSxc3nzJGF{e2G7	MWixNEDPxE1?	M3;CSFQ5KGh?		NFP0[HVz\WS3Y3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:\|ZT3k[ZBmdmSnboSgcYFvdmW{	M3K0NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEi3Olk4Lz5{MUi4O\|Y6PzxxYU6=
A375	NYXMOIZmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHHBSGoxNjVizszN	Mk\aNU82NzhiZB?=		Mli1bY5pcWKrdIOgdJJwdGmoZYLheIlwdiCjbnSgbY5lfWOnczDkbYZn\XKnboTpZZRqd25ib3[gcYVt[W6xbXGgZ4VtdHN?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd7Nk[yNkc,OjF5OU[2NlI9N2F-
SKMEL1	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGrCfoExNjVizszN	M1;JelEwPS96IHS=		NInjeIJqdmirYnn0d{Bxem:uaX\ldoF1cW:wIHHu[EBqdmS3Y3XzJIRq\m[ncnXueIlifGmxbjDv[kBu\Wyjbn;tZUBk\Wyucx?=	NXXWRlRSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3PVY3OjJpPkKxO\|k3PjJ{PD;hQi=>
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Ly 1	M3jBfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M37lXVI1KGh?		MlvDTWM2OD15LkOg{txO	NHWy[4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
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Su-DHL6	NInSelVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NGrFZY0zPCCq		MYTJR\|Ux97zgMkCg{txO	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
Ly 10	M176TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M4XrclI1KGh?		Mm\nTWM2OO,:nkKwJO69VQ>?	M2\qTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
RIVA	Mn\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NH\zT3YzPCCq		MVLJR\|Ux97zgMkCg{txO	NIPaPW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
Su-DHL2	NHKyc4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NXjxZ5AzOjRiaB?=		Mk\YTWM2OO,:nkKwJO69VQ>?	NIPP[FE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
Ly 1	M2PqS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		Mm\POFghcA>?		MYXJR\|UxRTBwM{Sg{txO	MnTUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5N{KwOFkoRjJzN{eyNFQ6RC:jPh?=
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Su-DHL6	MoDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NIfRNHE1QCCq		M{nFR2lEPTExvK6yNEDPxE1?	M17Ge\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
Ly 10	NXvaNJZGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M1v0N\|Q5KGh?		MWrJR\|UxRTFwODFOwG0>	M3jUWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
RIVA	NVuyPWx4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NWP2fZdUPDhiaB?=		NEPJZ4JKSzVy78{eNlAh\|ryP	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd5MkC0PUc,OjF5N{KwOFk9N2F-
Su-DHL2	M2PvNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NVTLXXpkPDhiaB?=		NYT5flZOUUN3ME2xO{41KM7:TR?=	NHTXS409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
Ly 1	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MlvaO\|IhcA>?		MlrSTWM2OD1yLkCxJO69VQ>?	Mo[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5N{KwOFkoRjJzN{eyNFQ6RC:jPh?=
Ly 7	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NFfxTow4OiCq		NITDSmxKSzVyPUCuNFE5KM7:TR?=	NHTIRlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
Su-DHL6	M{TUNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NHTT[mY4OiCq		NIfiRZJKSzVyPUGuOkDPxE1?	NUTDZ5JpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3O\|IxPDlpPkKxO\|czODR7PD;hQi=>
Ly 10	NXXrSmlIT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NV62e3J7PzJiaB?=		NGr4c5hKSzVyPUGuNkDPxE1?	M2TjdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{eyNFQ6Lz5{MUe3NlA1QTxxYU6=
RIVA	NVTVb2kxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NV3qW5UxPzJiaB?=		MkT4TWM2OO,:nkKwJO69VQ>?	NF3heZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
Su-DHL2	MlqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NVzxeZBpPzJiaB?=		NHHmNIVKSzVyPUGxMlIh\|ryP	NHfBT2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe3NlA1QSd-MkG3O\|IxPDl:L3G+
U373-MAGI	MnzERY51cX[rcnHsJIF{e2G7	MkHpNE4zPSC2bzC4JJVO	NFy0Z48zKHSxIEeyJIhzew>?		MmTYRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgWnNXNUdicIPleYRwfHmyZXSgTGlXNTFiTly0MVMhcW6oZXP0[YQhcW5iaIXtZY4hXTN5Mz3NRWdKKGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDVOU1o[WdibHX2[Ywh[XRiMD6yOUB1dyB6IIXNJIFnfGW{IEKgeI8hPzJiaILzJIJ6KHGSQ2KgcYV1cG:m	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI	NFO5fIhCdnSrdnnyZYwh[XO\|YYm=	MlnTNE4zPSC2bzC4JJVO	M{jkbVIhfG9iN{KgbJJ{		M{naWmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV\|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[4FoKGyndnXsJIF1KDBwMkWgeI8hQCC3TTDh[pRmeiB{IITvJFczKGi{czDifUByWEOUIH3leIhw\A>?	NVzvR4RTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	Survivin / Bcl-2 / p53 / c-Myc / DNMT1 ; p-AKT / AKT / p-GSK3β / GSK3β / p-Myc / Myc / p-P70 / P70 / p-4EBP-1 / 4EBP-1 / PTEN ; phospho-p38 / p38 / phospho-NFκB / NFκB ; p-JAK1 / JAK1 / p-JAK2 / JAK2 / p-STAT3 / STAT3 ; E-cadherin / N-cadherin / Snail / MMP-2 / MMP-9 / Bcl-2 / Bax	26384351 25762617 26617834 26692933 28152502
Immunofluorescence	DNMT1 ; E-cadherin / MMP-9	21303982 28152502
Growth inhibition assay	Cell viability	26384351

In vivo

In a ALK+ KARPAS-299 murine xenograft model, Decitabine at a dose of 2.5 mg/kg causes increased apoptosis and reduced proliferation of tumor cells, and also results in demethylation of tumor suppressor p16INK4A. ^[2]

Protocol (from reference)

Kinase Assay:^[1]	DNA synthesis assay : The rate of DNA synthesis is measured by the incorporation of radioactive thymidine into DNA. HL-60 and KG1a cells are suspended in 2 mL RPMI medium containing 10% fetal serum in 6-well (35 mm diameter) dishes and incubated with different concentrations of corresponding drugs for 48 hours (drugs are added simultaneously). At 48 hours, 0.5 μCi [³H] thymidine (6.7 Ci/mmol) is added to each well and incubated for an additional 24 hours. The cells are placed on GF/C glass fiber filters (2.4 cm diameter), washed with cold 0.9% NaCl, 5% cold trichloroacetic acid and ethanol. The filters containing the DNA are then dried, placed in EcoLite scintillation liquid (ICN) and the radioactivity measured using Beckman LS 6000IC scintillation counter. The IC50 is defined as the concentration of drug that inhibits 50% of the DNA synthesis of the leukemic cell lines from the dose–response curve.
Cell Research:^[1]	Cell lines: HL-60 and KG1a Concentrations: 0-500 nM Incubation Time: 96 hours Method: For the growth inhibition assay, cells in log phase are placed in 5 mL of medium. Different concentrations of Decitabine are added to the medium simultaneously. Cell counts are performed at the indicated times using a model ZM Coulter Counter. The concentration that produces 50% inhibition of growth (IC50) is determined from the growth curves of the drug treated leukemic cell lines.
Animal Research:^[2]	Animal Models: KARPAS-299 human cells are inoculated subcutaneously into the right and left flanks of the mice. Dosages: ≤2.5 mg/kg Administration: Administered via i.p.

References

Solubility (25°C)

In vitro


In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): water For best results, use promptly after mixing.	10mg/mL

Chemical Information

Molecular Weight	228.21
Formula	C₈H₁₂N₄O₄
CAS No.	2353-33-5
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1C(C(OC1N2C=NC(=NC2=O)N)CO)O

Download Decitabine (NSC 127716) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

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% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

Dilution Calculator Molecular Weight Calculator

Clinical Trial Information

NCT Number	Recruitment	Interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05360160	Not yet recruiting	Drug: SNDX-5613\|Drug: Venetoclax\|Drug: ASTX727	Acute Myeloid Leukemia	M.D. Anderson Cancer Center\|Astex Pharmaceuticals Inc.\|Syndax Pharmaceuticals Inc.	October 31 2022	Phase 1\|Phase 2
NCT05403450	Recruiting	Drug: Tolinapant\|Drug: Decitabine + Cedazuridine	Relapsed/Refractory Peripheral T-cell Lymphoma	Astex Pharmaceuticals Inc.	June 23 2022	Phase 1\|Phase 2
NCT04985656	Withdrawn	Drug: Pevonedistat\|Drug: Decitabine\|Drug: Cedazuridine	Myelodysplastic Syndromes (MDS)	Takeda	October 1 2021	Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
Is S1200 a racemic mixture or a monomer?

Answer:
S1200 is R form

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