Home Epigenetics DNA Methyltransferase inhibitor Gamma-Oryzanol For research use only.

Gamma-Oryzanol

Gamma-oryzanol (GO), a nutriactive phytochemical naturally occurring in crude rice bran oil, is an antioxidant compound used to treat hyperlipidemia, disorders of menopause and to increase the muscle mass. γ-Oryzanol is a potent DNA methyltransferases (DNMTs) inhibitor in the striatum of mice. γ-Oryzanol significantly inhibits the activities of DNMT1 (IC50=3.2 μM), DNMT3a (IC50=22.3 μM).

Gamma-Oryzanol Chemical Structure

Gamma-Oryzanol Chemical Structure

CAS: 11042-64-1

Selleck's Gamma-Oryzanol has been cited by 2 publications

Purity & Quality Control

Batch: S395701 DMSO] 100 mg/mL] false] ] ] false] ] ] false Purity: 96.54%
96.54

Gamma-Oryzanol Related Products

Signaling Pathway

DNA Methyltransferase Signaling Pathways

DNA Methyltransferase Signaling Pathway

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Biological Activity

Description Gamma-oryzanol (GO), a nutriactive phytochemical naturally occurring in crude rice bran oil, is an antioxidant compound used to treat hyperlipidemia, disorders of menopause and to increase the muscle mass. γ-Oryzanol is a potent DNA methyltransferases (DNMTs) inhibitor in the striatum of mice. γ-Oryzanol significantly inhibits the activities of DNMT1 (IC50=3.2 μM), DNMT3a (IC50=22.3 μM).
In vitro
In vitro From the proliferation assay using MTT reagent, the effect of gamma-oryzanol on DU145 and PC3 are slightly different with an IC50 after 7 days of exposure at 0.52±0.06 and 0.40±0.08 mg/ml, respectively. For in vitro condition in proliferation assay, IC50 values seem to be high in the scale of mg/ml. gamma-oryzanol may affect to prostate cancer cells through the down regulation of some antioxidant genes, CAT and GPX[1].
Cell Research Cell lines human DU145 and PC3 prostate cancer cells
Concentrations 0.1-2.0mg/ml
Incubation Time 24 h
Method The prostate cancer cells at a density of 3.5×104 cells/well in serum-containing medium are seeded in 24-well microplates and grown to 80-90% confluency. Then, they are treated with gamma-oryzanol at various concentrations in a humidified atmosphere of 5%CO2 at 37°C for 24h. After the incubation, cells are washed twice and 150μl of culture medium containing 1mg/ml of MTT dye is added to each well and further incubated for 4h. The medium containing MTT dye is then replaced with 150μl of DMSO. The blue color of the oxidized MTT (formazan) as determined by measuring the absorbance at 570 nm using ELISA microplate reader.
In Vivo
In vivo In tumor-bearing mice, gamma-oryzanol can reduce tumor mass associated with pro-angiogenic biomarkers[1]. Gamma-oryzanol is not carcinogenic and are safe for animals. In vivo trials proved gamma-oryzanol to be powerful antioxidant and also appears to be a very promising anticancerogenic compound. Supplementation of gamma-oryzanol in diabetic patients may improve metabolic dysfunctions such as hyperglycaemia, hypercholesterolaemia, hypertriglyceridaemia, insulin resistance and may alleviate diabetic complications resulting from lipid peroxidation and free radicals[3].

Chemical Information & Solubility

Molecular Weight 602.89 Formula

C40H58O4
CAS No. 11042-64-1 SDF Download Gamma-Oryzanol SDF
Smiles CC(CCC=C(C)C)C1CCC2(C1(CCC34C2CCC5C3(C4)CCC(C5(C)C)OC(=O)C=CC6=CC(=C(C=C6)O)OC)C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (165.86 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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