2',3'-cGAMP Sodium Salt

Catalog No.S7904 Synonyms: 2'-3'-cyclic GMP-AMP Sodium, 2',3'-cGAMP Sodium

For research use only.

2',3'-cGAMP Sodium Salt (2'-3'-cyclic GMP-AMP Sodium) is produced in response to DNA in the cytoplasm in mammalian cells and binds STING with high affinity and is an effective inducer of interferon-β (IFNβ). 2',3'-cGAMP binds to STING with Kd of 3.79 nM.

2',3'-cGAMP Sodium Salt Chemical Structure

CAS No. 1441190-66-4 (free acid)

Purity & Quality Control

Choose Selective STING Inhibitors

Biological Activity

Description 2',3'-cGAMP Sodium Salt (2'-3'-cyclic GMP-AMP Sodium) is produced in response to DNA in the cytoplasm in mammalian cells and binds STING with high affinity and is an effective inducer of interferon-β (IFNβ). 2',3'-cGAMP binds to STING with Kd of 3.79 nM.
Targets
STING [1]
(Cell-free assay)
3.79 nM(Kd)
In vitro

2',3'-cGAMP is an endogenous second messenger produced by mammalian cells. 2',3'-cGAMP is a high affinity ligand for STING. 2',3'-cGAMP is a potent inducer of type-I interferons. 2',3'-cGAMP binding induces conformational changes of STING.[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
293T MVTGeY5kfGmxbjDhd5NigQ>? M3PzcFMxKG2rboO= NIX6UlRC[3SrdnH0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIGPUTW5IKGijcHzveJlx\SCUN{HIM2czOzCDL2KyPVNSKG23dHHueEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczDt[YF{fXKnZDDh[pRmeiB|MDDtbY5{KGmwIIDy[ZNmdmOnIH;mJIRq\2m2b37pckBCKGK7IHLybYdpfCCJbH:tcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMjFOwG0v NF;WXnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUexOVA6QSd-M{G3NVUxQTl:L3G+
293T MlLGSpVv[3Srb36gZZN{[Xl? MUezNEBucW6| NHfLWWdC[3SrdnH0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIIfpcIQufHmyZTDTWGlPTyCneIDy[ZN{\WRiaX6gNlk{XCClZXzsd{Bu\WG|dYLl[EBi\nSncjCzNEBucW6|IHnuJJBz\XOnbnPlJI9nKGSrZ3n0c45qdiCDIHL5JIJzcWeqdDDHcI8udHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNkDPxE1w NYXjZlRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3NVUxQTlpPkOxO|E2ODl7PD;hQi=>
293T MULGeY5kfGmxbjDhd5NigQ>? M{PoR|MxKG2rboO= MYLBZ5RqfmG2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKFOWSV7HJIhieGyxdInw[UBIOjNyQT;SNlk{WSCvdYThcpQh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOgcYVie3W{ZXSgZYZ1\XJiM{CgcYlveyCrbjDwdoV{\W6lZTDv[kBlcWerdH;ubY4hSSCkeTDidolocHRiR3zvMYx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMESg{txONg>? NUfrfXF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3NVUxQTlpPkOxO|E2ODl7PD;hQi=>
293T NYC3TolzTnWwY4Tpc44h[XO|YYm= M2e2clMxKG2rboO= NXXNUlJNSWO2aY\heIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDTWGlPTyCqYYDsc5R6eGViUkK5N3EhdXW2YX70JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy|IH3lZZN2emWmIHHmeIVzKDNyIH3pcpMhcW5icILld4Vv[2Vib3[g[IlocXSxbnnuJGEh[nliYoLp[4h1KEeubz3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUCgQUAxNjB3IN88UU4> M2H2[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzN{G1NFk6Lz5|MUexOVA6QTxxYU6=
293T MoLwSpVv[3Srb36gZZN{[Xl? M2r4clMxKG2rboO= MmX3RYN1cX[jdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCVVFnOS{Bp[XCub4T5dIUhWjJ|MligcZV1[W62IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{KG2nYYP1doVlKGGodHXyJFMxKG2rboOgbY4heHKnc3XuZ4Uhd2ZiZHnnbZRwdmmwIFGgZpkh[nKrZ3j0JGdtdy2udXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTBiPTCwMlA4KM7:TT6= Mmi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF5MUWwPVkoRjNzN{G1NFk6RC:jPh?=
293T M3\3XWZ2dmO2aX;uJIF{e2G7 MnOwO{BpenN? M1fWSmFkfGm4YYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5id3ns[E11gXCnIGPUTW5IKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzJIlv[3WkYYTl[EBnd3JiNzDodpMhcW5iYXLz[Y5k\SCxZjDkbYdqfG:waX6gRUBjgSCkcnnnbJQhT2yxLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3MDC9JFE{NjdizszNMi=> NY\Te|VsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G3NVUxQTlpPkOxO|E2ODl7PD;hQi=>
THP1 MlPUSpVv[3Srb36gZZN{[Xl? MVGyNEBpenN? NXfycmU5SWexbnnzeEBi[3Srdnn0fUBifCCVVFnOS{BqdiCqdX3hckBVUFBzIHPlcIx{KGG|c3Xzd4VlKGG|IIP0bY12dGG2aX;uJI9nKEmURkOgdIF1cHejeTDt[YF{fXKnZDDh[pRmeiB{MDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyIE2gN|gvPiEQvF2u M1rsVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzOEKwPVg2Lz5|MUiyNFk5PTxxYU6=
PBMC MmDqSpVv[3Srb36gZZN{[Xl? NYnL[4FZPjlwNjD1US=> NVTSSXc5PCCqcoO= MlfxRYdwdmm|dDDhZ5Rqfmm2eTDheEBUXEmQRzDpckBpfW2jbjDQRm1EKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIFPYR2wyOCCvUl7BJIxmfmWuIHH0JFY6NjZidV2gcYVie3W{ZXSgZYZ1\XJiNDDodpMh[nlicWLUMXBEWiCjbnHsfZNqew>? NEfubVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUiyNFk5PSd-M{G4NlA6QDV:L3G+
PBMC M2D4S2Z2dmO2aX;uJIF{e2G7 MVuxMlM6KHSxIEGzPUB2VQ>? MWK0JIhzew>? NIj5Z3hC\2:waYP0JIFkfGm4aYT5JIF1KFOWSV7HJIlvKGi3bXHuJHBDVUNiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gTWZP[mW2YTDy[Yxm[XOnIHH0JFEvOzlidH:gNVM6KHWPIH3lZZN2emWmIHHmeIVzKDRiaILzJIJ6KEWOSWPB NIT2VZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUiyNFk5PSd-M{G4NlA6QDV:L3G+
PBMC MX7GeY5kfGmxbjDhd5NigQ>? MoPOOlkvPiC3TR?= MnW4OEBpenN? NF7VXJFC\2:waYP0JIFkfGm4aYT5JIF1KFOWSV7HJIlvKGi3bXHuJHBDVUNiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gTWZP[mW2YTDtVm5CKGyndnXsJIF1KDZ7Lk[geW0hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgdXJVNVCFUjDhcoFtgXOrcx?= NHHEcHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUiyNFk5PSd-M{G4NlA6QDV:L3G+
PBMC MkLFSpVv[3Srb36gZZN{[Xl? NV2yN|YyPjlwNjD1US=> M1;OclQhcHK| NU[4U4dCSWexbnnzeEBi[3Srdnn0fUBifCCVVFnOS{BqdiCqdX3hckBRSk2FIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGlNPiCvUl7BJIxmfmWuIHH0JFY6NjZidV2gcYVie3W{ZXSgZYZ1\XJiNDDodpMh[nlicWLUMXBEWiCjbnHsfZNqew>? M3vT[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzOEKwPVg2Lz5|MUiyNFk5PTxxYU6=
PBMC NU[wTpFwTnWwY4Tpc44h[XO|YYm= NGLjPWYyOzlidV2= MUO0JIhzew>? M{Lqe2Fod26rc4SgZYN1cX[rdImgZZQhW1SLTlegbY4hcHWvYX6gVGJOSyClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDJUFYheHKxZIXjeIlwdiCjdDCxN|khfU1ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhTUyLU1G= NXT5WHVMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G4NlA6QDVpPkOxPFIxQTh3PD;hQi=>
B16-OVA NHrZZW1CdnSrdIXtc5Ih[XO|YYm= NWmwVJlESW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhdW:3c3WgRlE3NU:YQTDj[YxteyCrbYDsZY51\WRiaX6gcY92e2ViYYPz[ZN{\WRiYYOgeJVud3W{IILl[5Jme3Orb36gbY4hcW6sZXP0[YQh\myjbnugZZQhOTBidXegZYRucW6rc4TldoVlKGmwdILheJVud3KjbHz5JI9vKGSjeTC2MEA6KGGwZDCxNkBxd3O2IHntdIxidnSjdHnvci=> M3P4NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUCwPVk3Lz5|MUWwNFk6PjxxYU6=
B16-OVA Mn;YRY51cXS3bX;yJIF{e2G7 MlrNRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQkG2MW9XSSClZXzsd{BqdXCuYX70[YQhcW5ibX;1d4Uh[XO|ZYPz[YQh[XNidIXtc5VzKHKnZ4Lld5Nqd25iaX6gZ49vfHKjbHH0[ZJidCCobHHub{BifCBzMDD1[{Bi\G2rbnnzeIVz\WRiaX70doF1fW2xcnHscJkhd25iZHH5JFYtKDliYX7kJFEzKHCxc4SgbY1xdGGwdHH0bY9v NFLtZpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUWwNFk6Pid-M{G1NFA6QTZ:L3G+
B16-OVA NX7NRoJySW62aYT1cY9zKGG|c3H5 MoHuRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQkG2MW9XSSClZXzsd{BqdXCuYX70[YQhcW5ibX;1d4Uh[XO|ZYPz[YQh[XNiaHnnbIVzKG63bXLldkBw\iCvb4Xz[UBkfXKnZDDv[kB1fW2xcoOgZZQhOTBidXegZYRucW6rc4TldoVlKGmwdILheJVud3KjbHz5JI9vKGSjeTC2MEA6KGGwZDCxNkBxd3O2IHntdIxidnSjdHnvci=> MoPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MEC5PVYoRjNzNUCwPVk3RC:jPh?=

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: A549, BSC-40, BSR-T7, Vero cells
  • Concentrations: 25 μM, 20 μM, 2.5 μM
  • Incubation Time: 30 min
  • Method:

    A549 cells are infected at an MOI of 5 with WT VACV or VACV-ΔPoxin and lysed 5 hpi. As a positive control, cells are permeabilized for 30 min with digitonin buffer (10 μg ml−1 digitonin, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 3 mM MgCl2, 0.1 mM DTT, 85 mM sucrose, 0.2% BSA, 1 mM ATP, and 0.1 mM GTP) and stimulated with or without 25 μM 2',3'-cGAMP. After stimulation, the cells are washed once with DMEM supplemented with 10% FBS, media is replaced, and cells are incubated 5 h before lysis.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 718.37
Formula

C20H22N10Na2O13P2

Density g/mL
CAS No. 1441190-66-4 (free acid)
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1C2C(C(C(O2)N3C=NC4=C3N=C(NC4=O)N)OP(=O)(OCC5C(C(C(O5)N6C=NC7=C(N=CN=C76)N)O)OP(=O)(O1)O)O)O

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