CCCP

Catalog No.S6494 Synonyms: Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

For research use only.

CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).

CCCP Chemical Structure

CAS No. 555-60-2

Selleck's CCCP has been cited by 12 Publications

Purity & Quality Control

Choose Selective STING Inhibitors

Biological Activity

Description CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).
In vitro

CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.[1]

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: cells freshly isolated from patient material or PDXs (patient-derived xenografts)
  • Concentrations: 25 µM
  • Incubation Time: 4 h
  • Method:

    5× 105 tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 204.62
Formula

C9H5ClN4

CAS No. 555-60-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=CC(=C1)Cl)NN=C(C#N)C#N

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05267808 Recruiting Procedure: Drug-induced sleep endoscopy Obstructive Sleep Apnea University Hospital Antwerp|Universiteit Antwerpen February 1 2022 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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