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CCCP

Synonyms: Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).

CCCP Chemical Structure

CCCP Chemical Structure

CAS: 555-60-2

Selleck's CCCP has been cited by 20 publications

Purity & Quality Control

Batch: Purity: 99.71%
99.71

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Biological Activity

Description CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).
In vitro
In vitro

CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.[1]
Cell Research Cell lines cells freshly isolated from patient material or PDXs (patient-derived xenografts)
Concentrations 25 µM
Incubation Time 4 h
Method

5× 105 tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.
In Vivo
In vivo

Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.
Animal Research Animal Models Female Balb/c mice
Dosages 3 mg/kg
Administration i.v.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05267808 Recruiting
Obstructive Sleep Apnea
University Hospital Antwerp|Universiteit Antwerpen
 February 1 2022 --

Chemical Information & Solubility

Molecular Weight 204.62 Formula

C9H5ClN4
CAS No. 555-60-2 SDF --
Smiles C1=CC(=CC(=C1)Cl)NN=C(C#N)C#N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 41 mg/mL ( (200.37 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 14 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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