Name: C-176
Brand: Selleck Chemicals
SKU: S6575
Availability: InStock
Rating: 5 (38 reviews)

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Quality Control

Batch: Purity: 99.98%

99.98

Related Targets	PD-1/PD-L1 CXCR AhR Immunology & Inflammation related CD markers Interleukins Anti-infection Antioxidant COX Histamine Receptor
Other STING Inhibitors	diABZI STING agonist-1 (tautomerism) H-151 CCCP STING inhibitor C-178 2',3'-cGAMP Sodium Salt SN-011 MSA-2 C-171 G10 (STING agonist-1) SN-001

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HCC1806	Cytotoxicity assay		3 days	Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 6.2 μM.	26116180
HCC38	Cytotoxicity assay		3 days	Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 8.7 μM.	26116180
HCC1143	Cytotoxicity assay		3 days	Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 9.5 μM.	26116180
HCC38	Cytotoxicity assay		3 days	Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	26116180
HEK293	Function assay	10 uM	24 hrs	Increase in CHOP mRNA expression in HEK293 cells at 10 uM incubated for 24 hrs by qRT-PCR method	26116180
HCC1806	Cytotoxicity assay		3 days	Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	26116180
HCC1143	Cytotoxicity assay		3 days	Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay	26116180
HEK293T	Function assay	10 uM	24 hrs	Increase in CHOP expression in HEK293T cells stably transfected with luciferase gene at 10 uM incubated for 24 hrs by luciferase reporter gene assay	26116180
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 72 mg/mL (201.06 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.895mg/ml (2.50mM)	Taking the 1 mL working solution as an example, add 50 μL of 17.9 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (2.79mM)	Taking the 1 mL working solution as an example, add 50 μL of 12 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	358.09	Formula	C₁₁H₇IN₂O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	314054-00-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=CC(=CC=C1NC(=O)C2=CC=C(O2)[N+](=O)[O-])I

Mechanism of Action

Targets/IC₅₀/Ki	STING
In vitro	C-176 strongly reduces STING-mediated, but not RIG-I- or TBK1-mediated, IFNβ reporter activity. It directly targets mouse STING (mmSTING) but not human STING (hsSTING).
In vivo	C-176 attenuates STING-associated autoinflammatory disease in mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/29973723/ [2] https://pubmed.ncbi.nlm.nih.gov/35584676/

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Handling Instructions

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C-176 STING inhibitor

Chemical Structure

Molecular Weight: 358.09