Home Immunology & Inflammation STING inhibitor C-176 For research use only.

C-176

STING inhibitor C-176 is a potent, small-molecule inhibitor of STING, a central signaling component of the intracellular DNA sensing pathway.

C-176 Chemical Structure

C-176 Chemical Structure

CAS: 314054-00-7

Selleck's C-176 has been cited by 24 publications

Purity & Quality Control

Batch: Purity: 99.98%
99.98

C-176 Related Products

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCC1806 Cytotoxicity assay 3 days Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 6.2 μM. 26116180
HCC38 Cytotoxicity assay 3 days Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 8.7 μM. 26116180
HCC1143 Cytotoxicity assay 3 days Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay, IC50 = 9.5 μM. 26116180
HCC38 Cytotoxicity assay 3 days Cytotoxicity against human HCC38 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay 26116180
HEK293 Function assay 10 uM 24 hrs Increase in CHOP mRNA expression in HEK293 cells at 10 uM incubated for 24 hrs by qRT-PCR method 26116180
HCC1806 Cytotoxicity assay 3 days Cytotoxicity against human HCC1806 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay 26116180
HCC1143 Cytotoxicity assay 3 days Cytotoxicity against human HCC1143 cells assessed as reduction in cell viability incubated for 3 days by cell-titer-Glo reagent based assay 26116180
HEK293T Function assay 10 uM 24 hrs Increase in CHOP expression in HEK293T cells stably transfected with luciferase gene at 10 uM incubated for 24 hrs by luciferase reporter gene assay 26116180
Click to View More Cell Line Experimental Data

Biological Activity

Description STING inhibitor C-176 is a potent, small-molecule inhibitor of STING, a central signaling component of the intracellular DNA sensing pathway.
Targets
STING [1]
In vitro
In vitro

C-176 strongly reduces STING-mediated, but not RIG-I- or TBK1-mediated, IFNβ reporter activity. It directly targets mouse STING (mmSTING) but not human STING (hsSTING)[1].
Cell Research Cell lines H526 cells
Concentrations 1 μM
Incubation Time 48 h
Method

Cells were treated 1 μM C176 and 1 μM AZD1775 for 48 h
In Vivo
In vivo

C-176 attenuates STING-associated autoinflammatory disease in mice[1].
Animal Research Animal Models C57BL/6J mice
Dosages 750 nmol/200 μL
Administration ip

Chemical Information & Solubility

Molecular Weight 358.09 Formula

C11H7IN2O4
CAS No. 314054-00-7 SDF --
Smiles C1=CC(=CC=C1NC(=O)C2=CC=C(O2)[N+](=O)[O-])I
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 72 mg/mL ( (201.06 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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