Home Apoptosis IAP inhibitor BV-6

BV-6

Catalog No.S7597

For research use only.

BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.

BV-6 Chemical Structure

CAS No. 1001600-56-1(freebase)

Selleck's BV-6 has been cited by 40 publications

Purity & Quality Control

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Biological Activity

Description BV-6 is a SMAC mimetic, dual cIAP and XIAP inhibitor.
Targets
cIAP [1] XIAP [1]
In vitro

BV6 inhibits the cell viability of HCC193 NSCLC cells with IC50 of 7.2 μM, induces apoptosis in both HCC193 and H460 cell lines, and also significantly enhances the radiosensitivity of these cell lines through activation of cleaved caspase-8 and cleaved caspase-9, respectively. [1] In immature dendritic cells, BV-6 treatment results in moderate activation of the classical NF-kB pathway. [2] Furthermore, BV-6 increases CIK cell-mediated lysis of hematological (H9, THP-1, and Tanoue) and solid malignancies (RH1, RH30, and TE671). BV-6 also enhances apoptosis of peripheral blood mononuclear cells and most notably has an inhibitory effect on immune cells limiting their cytotoxic potential. [3]
Assay
Methods Test Index PMID
Western blot cIAP1 / XIAP ; RIP1 / FADD / Caspase-8 ; p52 / RelB / p50 / p-p65 / Lamin B1 21760551 24287697
Growth inhibition assay Cell death 26775704

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HCC193 and H460 cells
  • Concentrations: ~30 μM
  • Incubation Time: ~48 hours
  • Method: Cell viability is measured using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay kit. 5000 cells/well are seeded into 96-well plates in triplicate. Following adhesion of cells to the wells, increasing concentrations of BV6 are added into different wells. Control groups are exposed to the same concentration of DMSO. The final concentrations of 333 μg/mL MTS and 25 μM PMS are added to each well 24 hours later. After two hours incubation at 37 °C in humidified 5% CO2, plates are read at the absorbance of 490-nm on a microplate reader. Relative cell viability of an individual sample is calculated by normalizing their absorbance to that of the corresponding control. IC50 values are calculated using Prism 5.01. For the TNFα neutralizing antibody assay, cells are exposed to 1 and 5 μM BV6 with or without 10 μg/mL infliximab and the assay is performed 24 hours later. Plates are read at the absorbance of 490-nm on a microplate reader
  • (Only for Reference)

Solubility (25°C)

In vitro

 DMSO 100 mg/mL
(82.94 mM)
Ethanol 100 mg/mL warmed
(82.94 mM)
Water 25 mg/mL
(20.73 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 1205.57
Formula

C70H96N10O8.xCF3COOH
CAS No. 1001600-56-1(freebase)
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C(=O)NC(C1CCCCC1)C(=O)N2CCCC2C(=O)NC(C(C3=CC=CC=C3)C4=CC=CC=C4)C(=O)NCCCCCCNC(=O)C(C(C5=CC=CC=C5)C6=CC=CC=C6)NC(=O)C7CCCN7C(=O)C(C8CCCCC8)NC(=O)C(C)NC)NC

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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