Catalog No.S7391 Synonyms: (1S)-Z-FA-FMK

For research use only.

Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.

Z-FA-FMK Chemical Structure

CAS No. 197855-65-5

Selleck's Z-FA-FMK has been cited by 8 Publications

Purity & Quality Control

Choose Selective Cysteine Protease Inhibitors

Biological Activity

Description Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
cysteine protease [1]
In vitro

Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. [2] Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. [3] Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. [4]

In vivo Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. [4] Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. [5]

Protocol (from reference)

Cell Research:[4]
  • Cell lines: PBMCs
  • Concentrations: ~100 μM
  • Incubation Time: 72 hours
  • Method: T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.

Solubility (25°C)

In vitro

DMSO 77 mg/mL
(199.26 mM)
Water Insoluble
Ethanol '34 mg/mL warmed

Chemical Information

Molecular Weight 386.42

C21 H23 F N2 O4

CAS No. 197855-65-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C(=O)CF)NC(=O)C(CC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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