Home Proteases Cysteine Protease inhibitor Z-FA-FMK

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Z-FA-FMK Cysteine Protease inhibitor

Cat.No.S7391

Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
Z-FA-FMK Cysteine Protease inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 386.42

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Chemical Information, Storage & Stability

Molecular Weight 386.42 Formula

C21 H23 F N2 O4

 Storage (From the date of receipt)
CAS No. 197855-65-5 Download SDF Storage of Stock Solutions

Synonyms (1S)-Z-FA-FMK Smiles CC(C(=O)CF)NC(=O)C(CC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2

Solubility

In vitro
Batch:

DMSO : 77 mg/mL (199.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 48 mg/mL

Water : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

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%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC50/Ki
cysteine protease [1]
In vitro
Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. [2] This compound inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. [3] It efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. [4]
In vivo
Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. [4] This compound blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. [5]
References
  • [4] https://pubmed.ncbi.nlm.nih.gov/16951345/
  • [5] https://pubmed.ncbi.nlm.nih.gov/20834102/

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