E-64

Catalog No.S7379

For research use only.

E-64 is an irreversible and selective cysteine protease inhibitor, and also inhibits papain, calpain, and cathepsins B and H, but not serine proteases or aspartic proteases. The IC50 for papain is 9 nM. E-64 induces oxidative stress and apoptosis in Filarial Parasite.

E-64 Chemical Structure

CAS No. 66701-25-5

Selleck's E-64 has been cited by 16 publications

Purity & Quality Control

Choose Selective Cysteine Protease Inhibitors

Biological Activity

Description E-64 is an irreversible and selective cysteine protease inhibitor, and also inhibits papain, calpain, and cathepsins B and H, but not serine proteases or aspartic proteases. The IC50 for papain is 9 nM. E-64 induces oxidative stress and apoptosis in Filarial Parasite.
Targets
Cysteine protease [1]
(Cell-free assay)
9 nM
In vitro

E-64 rapidly inhibits the activities of the cysteine proteinases cathepsins B, H and L and papain, while has no effect on the serine proteinases or the metalloproteinases. [2] E-64, as a specific inhibitor os cysteine proteases, effectively blocks in vitro ovarian cancer cell invasion. [3] In addition, E-64 also shows antiparasitic activity against Giardia lamblia excystation in vitro. [4] E-64 improves the preimplantation development of bovine somatic cell nuclear transfer embryos, which indicates another important implication of E-64. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human red blood cells NG[5fVFCdnSrcHzhd41w\GmjbDDhd5NigQ>? Mn\EOFghcHK| NWrCOIpbSW62aYDsZZNud2SrYXygZYN1cX[rdImgZYdicW6|dDDjbIxwem:zdXnu[U1z\XOrc4ThcpQhWGyjc33v[Il2dSCoYXzjbZBienWvIGeyJIlv\mWldHXkJIlvKGi3bXHuJJJm\CCkbH;v[EBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIILpcocue3SjZ3WgdIFz[XOrdHWg[5Jwf3SqIHHmeIVzKDR6IHjyd{BjgSCobH;3JIN6fG:vZYTyfUwhUUN3ME2xMlk1|ryP NH7KR3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5OVg5Pyd-MkGyPVU5QDd:L3G+
human red blood cells M12ydmFvfGmybHHzcY9lcWGuIHHzd4F6 Mnr4OFghcHK| NXqxRot1SW62aYDsZZNud2SrYXygZYN1cX[rdImgZYdicW6|dDDybY5oKHO2YXflJHBt[XOvb3TpeY0h\mGuY3nwZZJ2dSCZMjDpcoZm[3SnZDDpckBpfW2jbjDy[YQh[myxb3SgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KG[ub4egZ5l1d22ndIL5MEBKSzVyPUGuPVTPxE1? MoXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{G1NlIoRjJ{MEexOVIzRC:jPh?=
In vivo E-64 induces a marked decrease in the number of spontaneous metastasis in M5076 tumor bearing mice, while has no effect on the formation of experimental metastasis. [6] E-64 also shows antiparasitic activity against Giardia lamblia excystation in infected mice. [4]

Protocol (from reference)

Animal Research:

[5]

  • Animal Models: M5076 tumor bearing mice
  • Dosages: --
  • Administration: i.v.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 357.41
Formula

C15H27N5O5

CAS No. 66701-25-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy E-64 | E-64 supplier | purchase E-64 | E-64 cost | E-64 manufacturer | order E-64 | E-64 distributor