Loxistatin Acid (E-64C)

Catalog No.S7392 Synonyms: NSC 694279, EP 475

For research use only.

Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry.

Loxistatin Acid (E-64C) Chemical Structure

CAS No. 76684-89-4

Selleck's Loxistatin Acid (E-64C) has been cited by 3 Publications

Purity & Quality Control

Choose Selective Cysteine Protease Inhibitors

Biological Activity

Description Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. The cysteine protease cathepsin L is required for SARS-CoV-2 viral entry.
Targets
cysteine protease [1]
In vitro

Loxistatin Acid (E-64C), as a thiol protease inhibitor, inhibits calpain activity in intact platelets. [1]E-64C inhibits β-secretase activity in regulated secretory vesicles of neuronal chromaffin cells, and decrease Aβ production, indicating a therapeutic strategy for Alzheimer's disease. [4]

In vivo In pectoral muscle of dystrophic chickens, Loxistatin Acid (E-64C) normalizes the increased activities of cathepsin B and cathepsin H. [2] E-64C inhibits ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. [3]

Protocol (from reference)

Animal Research:[3]
  • Animal Models: Male Sprague-Dawley rats.
  • Dosages: ~400 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

DMSO 62 mg/mL
(197.21 mM)
Water 2 mg/mL
(6.36 mM)
Ethanol '62 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
saline
For best results, use promptly after mixing.

2mg/mL

Chemical Information

Molecular Weight 314.38
Formula

C15H26 N2 O5

CAS No. 76684-89-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)CCNC(=O)C(CC(C)C)NC(=O)C1C(O1)C(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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