research use only

Loxistatin Acid (E-64C) Cysteine Protease inhibitor

Name: Loxistatin Acid (E-64C)
Brand: Selleck Chemicals
SKU: S7392
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S7392

Loxistatin Acid (E-64C, NSC 694279, EP 475), a derivative of E-64, is an irreversible and membrane-permeant cysteine protease inhibitor. This compound inhibits cathepsin L, a cysteine protease required for SARS-CoV-2 viral entry.

Chemical Structure

Molecular Weight: 314.38

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: S739201 DMSO]62 mg/mL]false]Ethanol]62 mg/mL]false]Water]2 mg/mL]false Purity: 99.82%

99.82

Related Targets	HDAC Caspase MMP HCV Protease DPP Tyrosinase HIV Protease Proteasome Serine Protease Secretase PCSK9 Glutaminase Acyltransferase Thrombin Cathepsin B MALT PAD PREP Neprilysin SGK Aminopeptidase 3C-Like Protease PGDS GOT
Related Products	Aloxistatin (E64d) Calpeptin Odanacatib MG-101 (ALLN) E-64 Z-FA-FMK N-Ethylmaleimide (NEM) Cathepsin Inhibitor 1 PD 151746

Chemical Information, Storage & Stability

Molecular Weight	314.38	Formula	C₁₅H₂₆ N₂ O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	76684-89-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC 694279, EP 475			Smiles	CC(C)CCNC(=O)C(CC(C)C)NC(=O)C1C(O1)C(=O)O

Solubility

In vitro
Batch:

DMSO : 62 mg/mL ( (197.21 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 62 mg/mL

Water : 2 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	saline Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.000mg/ml (6.36mM)	Taking the 1 mL working solution as an example, add 2 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	cysteine protease ^[1]
In vitro	Loxistatin Acid (E-64C), as a thiol protease inhibitor, inhibits calpain activity in intact platelets. ^[1]It also inhibits β-secretase activity in regulated secretory vesicles of neuronal chromaffin cells, and decreases Aβ production, indicating a therapeutic strategy for Alzheimer's disease. ^[4]
In vivo	In pectoral muscle of dystrophic chickens, Loxistatin Acid (E-64C) normalizes the increased activities of cathepsin B and cathepsin H. ^[2] This compound also inhibits ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats. ^[3]
References	https://pubmed.ncbi.nlm.nih.gov/2537073/ https://pubmed.ncbi.nlm.nih.gov/7309707/ https://pubmed.ncbi.nlm.nih.gov/1706637/ https://pubmed.ncbi.nlm.nih.gov/14598316/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):