MG-101 (ALLN)

Catalog No.S7386 Synonyms: Calpain inhibitor-1, Ac-LLnL-CHO

For research use only.

MG-101 (ALLN, Calpain inhibitor-1, Ac-LLnL-CHO) is a cell-permeable and potent inhibitor of cysteine proteases including calpains and lysosomal cathepsins.

MG-101 (ALLN) Chemical Structure

CAS No. 110044-82-1

Selleck's MG-101 (ALLN) has been cited by 14 publications

Purity & Quality Control

Choose Selective Cysteine Protease Inhibitors

Biological Activity

Description MG-101 (ALLN, Calpain inhibitor-1, Ac-LLnL-CHO) is a cell-permeable and potent inhibitor of cysteine proteases including calpains and lysosomal cathepsins.
Cysteine protease [1]
In vitro

MG-101 (ALLN) effectively inhibits cysteine proteinases with ID50 of 7 nM and 13 nM for cathepsins L and B, respectively. MG-101 (ALLN) shows very weak inhibitory activities towards cathepsin D (aspartic protease) and subtilisin (serine protease). MG-101 thus transforms NIH3T3 cells and also induces differentiation of PC12 pheochromocytoma cells. [1] MG-101, as an inhibitor of Ca(2+)-dependent cysteine proteases, inhibits the degradation of HMG-CoA reductase and HMGal in cholesterol biosynthesis. [2] In HCT116 cells, MG-101 decreases cell viability and tumor growth, and induces apoptosis response through Bax translocation from cytosol to mitochondria. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 MUDGeY5kfGmxbjDhd5NigQ>? NVHye4lDUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJI9nKHSqZTDjc41xd3WwZDD3ZZMh\GW2ZYLtbY5m\CCjZ3HpcpN1KExzMkGwJINmdGy|LDDJR|UxRTQQvF2u NFfDdpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[zOFAzQCd-MUW2N|QxOjh:L3G+
melanoma B16 MoHzSpVv[3Srb36gZZN{[Xl? NYPPT3RmUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJI9nKHSqZTDjc41xd3WwZDD3ZZMh\GW2ZYLtbY5m\CCjZ3HpcpN1KG2nbHHuc41iKEJzNjDj[YxteyxiSVO1NF0yPC53zszNMi=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ|NECyPEc,OTV4M{SwNlg9N2F-
HeLa MnjuR5l1d3SxeHnjbZR6KGG|c3H5 Mk\VOFghcHK| Ml3lR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA1QCCqcoOgZpkhVVSVIHHzd4F6NCCFQ{WwQVI2NjIQvF2u M2nXUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{W3OlMzLz5{M{O1O|Y{OjxxYU6=
HEK293 NE\1bXhHfW6ldHnvckBie3OjeR?= M{W3bWlvcGmkaYTpc44hd2ZicILveIVie2:vZTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIVn\mWldDDvckBEgWOuaX7ENU1ETEt2IHnueIVz[WO2aX;uJINwdXCuZYjld{BjgSCHWV\QJIFv\C:xcjDZSnAhXmWwdYOg[pJi\22nboSgZoF{\WRicnXwc5J1\XJiZ3Xu[UBie3OjeR?= MkHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293 NGi0N3NHfW6ldHnvckBie3OjeR?= MY\Jcohq[mm2aX;uJI9nKHC{b4TlZZNwdWViZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDl[oZm[3Rib36gVIlvOS2MdX6gbY51\XKjY4Tpc44h[2:vcHzlfIV{KGK7IFXZSnAh[W6mL3;yJHlHWCCYZX71d{BnemGpbXXueEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG|c3H5 MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293 NYX3T4V[TnWwY4Tpc44h[XO|YYm= Mkj5TY5pcWKrdHnvckBw\iCycn;0[YF{d22nIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOg[YZn\WO2IH;uJJA2Oy2yNUOgbY51\XKjY4Tpc44h[2:vcHzlfIV{KGK7IFXZSnAh[W6mL3;yJHlHWCCYZX71d{BnemGpbXXueEBj[XOnZDDy[ZBwenSncjDn[Y5mKGG|c3H5 NUnsTZY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[2PFAyPTlpPkG2OlgxOTV7PD;hQi=>
HEK293 Ml7oSpVv[3Srb36gZZN{[Xl? NF7DSlZG\m[nY4Sgc44h[2:oaXzpclEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCnZn\lZ5Qhd25iY3;mbYxqdjF9IFzpcYszKGmwdHXyZYN1cW:wIHPvcZBt\XinczDifUBGYU[SIHHu[E9weiC\RmCgWoVvfXNiZoLh[41mdnRiYnHz[YQhemWyb4L0[ZIh\2WwZTDhd5NigQ>? Ml\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
HEK293 NVPaZ4I4TnWwY4Tpc44h[XO|YYm= NGjtSnFKdmirYnn0bY9vKG:oIIDyc5Rm[XOxbXWg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bm\m[nY4Sgc44hTTZ8RU\BVEBqdnSncnHjeIlwdiClb33wcIV5\XNiYomgSXlHWCCjbnSvc5IhYU[SIG\lcpV{KG[{YXft[Y51KGKjc3XkJJJmeG:{dHXyJIdmdmViYYPzZZk> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6MEG1PUc,OTZ4OECxOVk9N2F-
HEK293 M4[5c2Z2dmO2aX;uJIF{e2G7 NF:5bXJKdmirYnn0bY9vKG:oIIDyc5Rm[XOxbXWg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bm\m[nY4Sgc44heDV|LVPob|EhcW62ZYLhZ5Rqd25iY3;tdIxmgGW|IHL5JGV[TlBiYX7kM49zKFmIUDDW[Y52eyCocnHncYVvfCCkYYPl[EBz\XCxcoTldkBo\W6nIHHzd4F6 Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ4OECxOVkoRjF4NkiwNVU6RC:jPh?=
Methods Test Index PMID
Western blot Cyclin D3 / Cyclin D1 / CDK4 / CDK6 ; p21(WAF1/CIP1) 28130444
In vivo In mice bearing HCT116 xenografts, MG-101 (10 mg/kg i.p.) inhibits colon tumor formation. [3]

Protocol (from reference)

Cell Research:[3]
  • Cell lines: HCT116, HCT116/p53−/−, and HCT116/Bax−/− cells
  • Concentrations: ~26 μM
  • Incubation Time: 24 hours
  • Method: Cells viability is assayed using Cell Counting Kit-8 following manufacturer’s protocol. All HCT116 cell typesare seeded into each well of 96-well plate, cultured to 80% density and treated with different doses of ALLN for 24 h. Medium is then replaced by 100 μl fresh McCoy’s 5A complete medium with 10% CCK-8 reagent and incubated for 1 h. Absorbance is measured at 450 nm using a microplate reader. Results are shown as death percentages.
  • (Only for Reference)
Animal Research: [3]
  • Animal Models: Female athymic nude mice bearing HCT116 xenografts
  • Dosages: 10 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 76 mg/mL
(198.15 mM)
Water Insoluble
Ethanol '76 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 383.53


CAS No. 110044-82-1
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04987242 Recruiting Drug: ALLN-346|Drug: Placebo Hyperuricemia|Gout Allena Pharmaceuticals July 16 2021 Phase 2
NCT04829435 Completed Drug: ALLN-346|Drug: Placebo Hyperuricemia|Gout Allena Pharmaceuticals April 21 2021 Phase 1
NCT04236219 Completed Drug: ALLN-346|Drug: Placebo Hyperuricemia Allena Pharmaceuticals September 2 2020 Phase 1

(data from, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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