N-Ethylmaleimide (NEM)

Catalog No.S3692

For research use only.

N-Ethylmaleimide (NEM) is an organic compound that is derived from maleic acid. It is an irreversible inhibitor of all cysteine peptidases, with alkylation occurring at the active site thiol group. N-Ethylmaleimide (NEM) inactivates endogenous deubiquitinating enzymes (DUBs). N-Ethylmaleimide (NEM) specifically inhibits phosphate transport in mitochondria.

N-Ethylmaleimide (NEM) Chemical Structure

CAS No. 128-53-0

Selleck's N-Ethylmaleimide (NEM) has been cited by 6 Publications

Purity & Quality Control

Choose Selective Cysteine Protease Inhibitors

Biological Activity

Description N-Ethylmaleimide (NEM) is an organic compound that is derived from maleic acid. It is an irreversible inhibitor of all cysteine peptidases, with alkylation occurring at the active site thiol group. N-Ethylmaleimide (NEM) inactivates endogenous deubiquitinating enzymes (DUBs). N-Ethylmaleimide (NEM) specifically inhibits phosphate transport in mitochondria.
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KBV1 MV\DfZRwfG:6aXPpeJkh[XO|YYm= MV[3NkBpenN? M4\6fWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGS{dXetdoV{cXO2YX70JIh2dWGwIFvCWlEh[2WubIOg[ZhxemW|c3nu[{BRNWeyIHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPs7:TR?= NIDpbJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[1O|I4OSd-MkG2OVczPzF:L3G+
KB-3-1 MWnDfZRwfG:6aXPpeJkh[XO|YYm= MUC3NkBpenN? NF7ON3lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSi1|LUGgZ4VtdHNiaX7jeYJifGWmIH\vdkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVMx|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ3N{K3NUc,OjF4NUeyO|E9N2F-
COS7 Ml3ZSpVv[3Srb36gZZN{[Xl? NX[0SHVXQTBibXnudy=> Mnr4Rolv\GmwZzDh[oZqdmm2eTD0c{Bz[XRiR3HsdIhiNW[3c3XkJG9TVDFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJIFnemmlYX6g[5Jm\W5ibX;ub4V6KEORU{egZ4VtdHNiYX\0[ZIhQTBibXnud{whTUN3ME20N{44|ryP NHrDNlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC2NVgzOyd-MkKwOlE5OjN:L3G+
BL21 (DE3) NX6wcnNJTnWwY4Tpc44h[XO|YYm= NYTXUYdDOzBibXnudy=> MYrJdpJmfmW{c3nicIUhcW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDJSGUh\XiycnXzd4VlKGmwIFXzZ4hmemmlaHnhJINwdGliQlyyNUApTEV|KTDj[YxteyC3c3nu[{BCXFSRIE[1OU0hS3m|LVz5d{1N\XVvVnHsMXBp\S2SaHWtRYxiNUeudT3Bd5AuXHKyIHHzJJN2[nO2cnH0[UBxemWrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkhOTByIH\vcIQh[2:vcH;1coQh\GmudYTpc44h[nlic4DlZ5Rzd3Cqb4TvcYV1emmlIHHuZYw> NGjKXoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUK3OlkxOCd-M{GyO|Y6ODB:L3G+
KU812 NXL5UZJvTnWwY4Tpc44h[XO|YYm= NUfnWHN3OjBibXnudy=> NXu1fmFlUW6qaXLpeIlwdiCxZjDTSW5ROSCrbjDoeY1idiCNVUixNkBk\WyuczDwdoVqdmO3YnH0[YQh\m:{IEKwJI1qdnNiZn;scI94\WRiYomgZYRlcXSrb36gc4YhUEFvU2XNU|EuXlNiYYOgd5Vje3S{YYTlJI1m[XO3cnXkJIFnfGW{IEKgbJJ{KGK7IHntcZVvd2Kub4SgZY5idHm|aYO= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzR7MUexNEc,Ojd2OUG3NVA9N2F-

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 25 mg/mL
(199.79 mM)
Water 25 mg/mL
(199.79 mM)
Ethanol 25 mg/mL
(199.79 mM)

Chemical Information

Molecular Weight 125.13
Formula

C6H7NO2

CAS No. 128-53-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCN1C(=O)C=CC1=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00750477 Completed Dietary Supplement: NEM|Dietary Supplement: Placebo Osteoarthritis ESM Technologies LLC December 2004 Not Applicable
NCT00750230 Completed Dietary Supplement: NEM Osteoarthritis|Fibromyalgia ESM Technologies LLC October 2003 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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