Calpeptin

For research use only.

Catalog No.S7396

13 publications

Calpeptin Chemical Structure

CAS No. 117591-20-5

Calpeptin is a potent, cell-permeable calpain inhibitor with ID50 of 52 nM, 34 nM, 138 nM, and 40 nM for Calpain I (porcine erythrocytes), Calpain II (porcine kidney), Papainb, and Calpain I (human platelets), respectively. Calpeptin attenuates apoptosis and intracellular inflammatory changes in muscle cells.

Selleck's Calpeptin has been cited by 13 publications

1 Customer Review

  • Western blotting detection of focal adhesion kinase (FAK) expression and its cleaved fragments with different treatments. DTX represents docetaxel, CPP represents calpeptin (a calpain inhibitor). The experiments were carried out 3 times, obtaining essentially similar results.

    Oncol Rep, 2017, 37(6):3651-3659. Calpeptin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Calpeptin is a potent, cell-permeable calpain inhibitor with ID50 of 52 nM, 34 nM, 138 nM, and 40 nM for Calpain I (porcine erythrocytes), Calpain II (porcine kidney), Papainb, and Calpain I (human platelets), respectively. Calpeptin attenuates apoptosis and intracellular inflammatory changes in muscle cells.
Targets
Calpain II (porcine kidney) [1]
()
Calpain I (human platelets) [1]
()
Calpain I (porcine erythrocytes) [1]
()
Papainb [1]
()
34 nM(ID50) 40 nM(ID50) 52 nM(ID50) 138 nM(ID50)
In vitro

Calpeptin inhibits 20K phosphorylation in a dose-related manner in platelets stimulated by thrombin, ionomycin or collagen. [1] In differentiating PC12 cells, Calpeptin promotes neurite elongation via inhibition of Calpain activity. [2] In rat retinal ganglion cells, Calpeptin attenuates apoptosis, maintains normal whole-cell membrane potential, and thus provides functional neuroprotection. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 NGPBV49CfXSxcHjh[5kh[XO|YYm= NFPCcYEyOCC3TR?= MkfhPVYhcHK| M4HtNWlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6geY5lcW[oZYLlcpRq[XSnZDDyZZQhe3SjYnzlJIlv\HWlaXLs[UBRSzF{IHPlcIx{KGW6cILld5NqdmdiRVfGVE1JTFF5NDDhd5Nme3OnZDDhd{BGT0[SLVjEVVc1KGOuZXHyZY5k\SCjdDCxNEB2VSCjZoTldkA6PiCqcoOgZpkh[2GucHHpckBi[3SrdnH0bY9vKGG|c3H5 MmntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUG5OFkoRjF6M{mxPVQ6RC:jPh?=
PC12 Ml62RZV1d3CqYXf5JIF{e2G7 NHe0eYsyOCC3TR?= NVnHZYZGQTZiaILz NFjmUVhKdmS3Y4Tpc44hd2ZiYYX0c5Bp[We7IHnuJIRq\m[ncnXueIlifGWmIILheEB{fGGkbHWgbY5lfWOrYnzlJHBEOTJiY3XscJMh\XiycnXzd4lv\yCDNUPUJIFteGijLYP5cpVkdGWrbjDhd5Nme3OnZDDhd{BCPTOWIHHsdIhiNXO7boXjcIVqdiClbHXhdoFv[2ViYYSgNVAhfU1iYX\0[ZIhQTZiaILzJIJ6KGOjbIDhbY4h[WO2aY\heIlwdiCjc4PhfS=> NIDMflI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5NVk1QSd-MUizPVE6PDl:L3G+
COS7 MVvBeZRweGijZ4mgZZN{[Xl? NUWxW3F2PTBidV2= NHjtfVk1QCCqcoO= NWLy[oN7UW6mdXP0bY9vKG:oIHH1eI9xcGGpeTDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy|IHX4dJJme3OrbnegSWdHWC2KRGG3OEBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHBNSy2ncIPpcI9vKG:4ZYLlfJBz\XO|aX;uMYlv\HWlZXSgSWdHWC2KRGG3OEBi\2e{ZXfheIlwdiCjdDC1NEB2VSCjZoTldkA1QCCqcoO= MmDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUG5OFkoRjF6M{mxPVQ6RC:jPh?=
PC12 Ml\mRZV1d3CqYXf5JIF{e2G7 MmPENVAhfU1? MnT0PVYhcHK| MYTJcoR2[3Srb36gc4Yh[XW2b4DoZYd6KGmwIIXu[Iln\mW{ZX70bYF1\WRicnH0JJN1[WKuZTDpcoR2[2mkbHWgVGMyOiClZXzsd{BmgHC{ZYPzbY5oKEF3M2SgZYxxcGFvc4nueYNt\WmwIHHzd4V{e2WmIHHzJGE2O1RiYXzwbIEue3mwdXPs[YlvKGOuZXHyZY5k\SCjdDCxNEB2VSCjZoTldkA6PiCqcoOgZpkh[2GucHHpckBi[3SrdnH0bY9vKGG|c3H5 MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7MUm0PUc,OTh|OUG5OFk9N2F-
PC12 MkHnRZV1d3CqYXf5JIF{e2G7 NX24dHMzOTBidV2= M4HhZlk3KGi{cx?= MmfjTY5lfWO2aX;uJI9nKGG3dH;wbIFogSCrbjDkbYZn\XKnboTpZZRm\CC{YYSgd5Ri[mynIHnu[JVkcWKuZTDQR|EzKGOnbHzzJIV5eHKnc4PpcochTUeIUD3ISHE4PCCjc4Pld5Nm\CCjczDFS2ZRNUiGUUe0JINt\WG{YX7j[UBifCBzMDD1UUBi\nSncjC5OkBpenNiYomgZ4FteGGrbjDhZ5RqfmG2aX;uJIF{e2G7 M3v1bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{mxPVQ6Lz5zOEO5NVk1QTxxYU6=
HeLa NFO2R45CfXSxcHjh[5kh[XO|YYm= NWq1OZlWPTBidV2= NIW3boZKdmS3Y4Tpc44hd2ZiYYX0c5Bp[We7IHnuJIh2dWGwIFjlUIEh[2WubIOg[ZhxemW|c3nu[{BGT0[SLVzDN{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDMR|MuOiCuZY\lcEBifCB3MDD1UUBqdiCycnXz[Y5k\SCxZjC0NFAhdk1iYnHmbYxwdXmlaX6gRVE> Mn[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUG5OFkoRjF6M{mxPVQ6RC:jPh?=
COS7 M{nuPWF2fG:yaHHnfUBie3OjeR?= M4\nU|UxKHWP M3nCdFI1KGi{cx?= M3TKbWlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS0:VNzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBifXSxcHjh[49{d22nIHH0JFUxKHWPIHHmeIVzKDJ2IHjyd{BjgSCnbHXjeJJwdiCvaXPyc5Nkd3C7 MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7MUm0PUc,OTh|OUG5OFk9N2F-
COS7 NXLW[I5ESXW2b4DoZYd6KGG|c3H5 M4XW[|UxKHWP NXTSe5hIPDhiaILz M361Z2lv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS0:VNzDj[YxteyCneIDy[ZN{cW6pIFXHSnAuUESTN{Svdohm[iCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hTUeIUD3ISHE4PCCjZ3fy[YdifGmxbjDheEA2OCC3TTDh[pRmeiB2ODDodpM> Mn;oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUG5OFkoRjF6M{mxPVQ6RC:jPh?=
COS7 NX3YWVNRSXW2b4DoZYd6KGG|c3H5 MknPOVAhfU1? NUD3TXdTOjRiaILz NWrqbo1vUW6mdXP0bY9vKG:oIHH1eI9xcGGpeTDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCFT2O3JINmdGy|IHX4dJJme3OrbnegSWdHWC2OQ{OgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iRVfGVE1NSzNidnXzbYNt\SCjdDC1NEB2VSCjZoTldkAzPCCqcoO= M{\zSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{mxPVQ6Lz5zOEO5NVk1QTxxYU6=
HeLa MnixRY51cX[rcnHsJIF{e2G7 MUHBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDoeY1idiC{aHnuc5ZqenW|LUG0JIlvKEinTHGgZ4VtdHNuIFnDOVA:OTdwN988UU4> M{XnNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFkzPzB6Lze+R4hGVUKOPD;hQi=>
HeLa MlzRR5l1d3SxeHnjJIF{e2G7 NWLCN|JTS3m2b4TvfIlkKGGldHn2bZR6KGGpYXnud5QhUGWOYTDj[YxteyC3c3nu[{BZXFRiYYPzZZktKFSFNUC9NlIvOc7:TT6= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5NlcxQC9pPlPoSW1DVDxxYU6=
NMuMG M1jMVmZ2dmO2aX;uJIF{e2G7 MYWyOUB1dyB3MDD1US=> M2K2U|I1KHSxIEeyJIhzew>? Mk[3TY5pcWKrdHnvckBw\iClYXzwZYlvKGmwIH3veZNmKE6PdV3HJINmdGy|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIGTHSoJmfGFzLXnu[JVk\WRiY3HsdIFqdiCjY4Tpeol1gSCkeTDt[YF{fXKrbnegSolt[W2rbj3BJIxmfmWuczDheEAzPSC2bzC1NEB2VSCrbnP1ZoF1\WRiZn;yJFI1KHSxIEeyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> Mn\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVI4ODhxJ{7DbGVOSkx:L3G+
NMuMG MXLGeY5kfGmxbjDhd5NigQ>? NYXRZ|VDOjVidH:gOVAhfU1? M4TtSlI1KHSxIEeyJIhzew>? MlXyTY5pcWKrdHnvckBw\iClYXzwZYlvKGmwIH3veZNmKE6PdV3HJINmdGy|IHHzd4V{e2WmIHHzJJN2eHC{ZYPzbY9vKG:oIGTHSoJmfGFzLXnu[JVk\WRiRV3UJIJ6KG2nYYP1dolv\yCjbIDoZU1UVUFiZYjwdoV{e2mxbjDheEAzPSC2bzC1NEB2VSCrbnP1ZoF1\WRiZn;yJFI1KHSxIEeyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NGPCZY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6OjdyOD:nQmNpTU2ETEyvZV4>

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Assay
Methods Test Index PMID
Immunofluorescence
Src / EGFR ; 

PubMed: 29641465     


Immunocytochemical analysis for determining the effect of calcium-mediated calpain activity on Src and EGFR expression in NSCLC cells. Calpeptin is a protein belonging to the family of calcium-dependent proteolytic enzymes, which was used for confirming the calcium-mediated effect of LCS on NSCLC.

PubMed: 29641465     


Immunocytochemical analysis for evaluating the effect of calcium-mediated calpain activity on α-tubulin expression in NSCLC cells. Calpeptin is a protein belonging to the family of calcium-dependent proteolytic enzymes, which was used for confirming the calcium-mediated effect of LCS on NSCLC.

29641465
In vivo In a feline right ventricular (RV) PO (RVPO) model, calpeptin (0.6 mg/kg, i.v.) blocks the activation of calpain and caspase-3, cleavage of their substrates, and cardiomyocyte programmed cell death. [4] In a rat focal cerebral ischemia–reperfusion injury model, Calpeptin reduces the neuronal apoptosis in hippocampal CA1 sector via inhibition of the expression of Caspase-3. [5]

Protocol

Animal Research:[4]
- Collapse
  • Animal Models: Feline right ventricular (RV) PO (RVPO) model
  • Dosages: 0.6 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (198.64 mM)
Ethanol 72 mg/mL (198.64 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 362.46
Formula

C20H30N2O4

CAS No. 117591-20-5
Storage powder
in solvent
Synonyms N/A
Smiles CCCCC(C=O)NC(=O)C(CC(C)C)NC(=O)OCC1=CC=CC=C1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID