Ilomastat (GM6001, Galardin)

For research use only.

Catalog No.S7157

35 publications

Ilomastat (GM6001, Galardin) Chemical Structure

CAS No. 142880-36-2

Ilomastat (GM6001, Galardin) is a broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-12, MMP-14, and MMP-26 with Ki of 0.4 nM, 0.5 nM, 27 nM, 3.7 nM, 0.1 nM, 0.2 nM, 3.6 nM, 13.4 nM, 0.36 nM, respectively.

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10mM (1mL in DMSO) USD 259 In stock
USD 250 In stock
USD 750 In stock
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Selleck's Ilomastat (GM6001, Galardin) has been cited by 35 publications

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Biological Activity

Description Ilomastat (GM6001, Galardin) is a broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-12, MMP-14, and MMP-26 with Ki of 0.4 nM, 0.5 nM, 27 nM, 3.7 nM, 0.1 nM, 0.2 nM, 3.6 nM, 13.4 nM, 0.36 nM, respectively.
MMP-8 [1]
(Cell-free assay)
MMP-9 [1]
(Cell-free assay)
MMP-26 [1]
(Cell-free assay)
MMP-1 [1]
(Cell-free assay)
MMP-2 [1]
(Cell-free assay)
0.1 nM(Ki) 0.2 nM(Ki) 0.36 nM(Ki) 0.4 nM(Ki) 0.5 nM(Ki)
In vitro

GM6001 inhibits human skin fibroblast collagenase with Ki of 0.4 nM when assayed with a synthetic thio ester substrate at pH 6.5, with 50-fold selectivity over two bacterial enzymes, thermolysin and Pseudomonas aeruginosa elastase. [1] GM 6001 (0.1 nM - 10 nM) inhibits gelatinase A and gelatinase B produced by T-cells, thus inhibits T-cell homing. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEk293 cells NUnT[|ZQTnWwY4Tpc44h[XO|YYm= MUnJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEGGQV3UV|Qh\XiycnXzd4VlKGmwIFjFb|I6OyClZXzsd{B2e2mwZzDGRW0uSUW5TGHHVnBKW0mDSz3URW1TSSCjczDzeYJ{fHKjdHWgcYVie3W{ZXSg[o9zKDF3IH3pcpMh[nliZnz1c5JwdWW2cnnjJIFv[Wy7c3nzMEBKSzVyPUCuOFgh|ryP M4DuZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{e2PVk4Lz5{M{O3Olk6PzxxYU6=
U251 cells NH3PR5NHfW6ldHnvckBie3OjeR?= MUmyNEDPxE1? NY\4XHhPWHKxdHXjeIlwdiCjZ3HpcpN1KGGwdHjyZZghWEF4Mz3s[ZRp[WxidH;4bY4h[2:vcHzlfE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gSCrbjDoeY1idiCXMkWxJINmdGy|IHH0JFIxKHWPIHH0JFQxOCCwZz;tcEBjgSCPVGSgeIV{fA>? Mn3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3M{e3NlEoRjF5NUO3O|IyRC:jPh?=
HEk293 cells NVGySlZ[TnWwY4Tpc44h[XO|YYm= M2rtUmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiQVTBUXRUPSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KHW|aX7nJGFjgi2WRWPFe3NTT0GLWT3EdIEuU0tiYYOgd5Vje3S{YYTlJI1m[XO3cnXkJIZweiB{IHjyd{BjgSCobIXvdo9u\XS{aXOgZY5idHm|aYOsJGlEPTB;MD61JO69VQ>? MnPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{[5PVcoRjJ|M{e2PVk4RC:jPh?=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
MyoD / Myf5 / Myf6 / M-Cadherin / Notch1; 

PubMed: 23329998     

Gene expression was examined for 4 genes related to myogenic differentiation (MyoD, Myf5, Myf6 and M-cadherin) and 1 signaling gene (Notch1) after 3 and 6 hours pretreatment with GM6001 and 1 day cultured with myogenic differentiation meda. GAPDH was used䲧疝Ỵ疞㧀疜膉痘 

CD34 / Sca1; 

PubMed: 23329998     

The gene expression of MDSCs treated with 25μM of GM6001 for 3 and 6 hours. These results indicate that MDSCs treated with an MMP inhibitor had reduced expression of stem cell markers, CD34 and Sca1. GAPDH was used as a loading control.

ZO-1 / Claudin-5 / occludin; 

PubMed: 21857898     

(A) Confocal imaging of ZO-1, claudin-5 and occludin of human BMEVCs; (B, C, D) BMEVCs co-cultured with SHI-1, HL-60 and U937 cells with or without treatment by GM6001. (E) BMEVCs of co-cultured with SHI-1 cells after knock-down of the MMP-2/MMP-9 gene. D䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸

In vivo Topical application of GM6001 (400 μg/ml) prevents corneal ulceration after severe alkali injury. [3] In rabbit model after stenting, GM6001 significantly inhibits intimal hyperplasia and intimalcollagen content, and it increases lumen area in stented arteries without effects on proliferation rates. [4]


Animal Research:


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  • Animal Models: Rabbit
  • Dosages: 100 mg/kg/day
  • Administration: subcutaneous
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 78 mg/mL (200.79 mM)
Ethanol 8 mg/mL warmed (20.59 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+40% PEG 300+2% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 388.46


CAS No. 142880-36-2
Storage powder
in solvent
Synonyms N/A
Smiles CC(C)CC(CC(=O)NO)C(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC

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MMP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID