SB-3CT

Catalog No.S7430 Batch:S743004

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Technical Data

Formula

C15H14O3S2
Molecular Weight 306.40 CAS No. 292605-14-2
Solubility (25°C)* In vitro DMSO 61 mg/mL (199.08 mM)
Ethanol 10 mg/mL (32.63 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SB-3CT is an effective and selective gelatinase inhibitor with Ki of 13.9 nM and 600 nM for MMP-2 and MMP-9, respectively.
Targets
MMP-2 [1]
(Cell-free assay)		 MMP-9 [1]
(Cell-free assay)
13.9 nM(Ki) 600 nM(Ki)
In vitro

SB-3CT directly inhibits bone marrow endothelial cell invasion and tubule formation in Matrigel in vitro. [3]
In vivo

In L-CI.5s T-cell lymphoma model, SB-3CT (5-50 mg/kg/d i.p.) potently inhibit liver metastasis and increases survival of mice. [2]

SB-3CT (50 mg/kg/d i.p.) inhibits human prostate cancer growth, osteolysis and angiogenesis in a bone metastasis model. [3]

In a mouse model of embolus-induced “permanent” focal cerebral ischemia, SB-3CT counteracts degradation of neuronal laminin, protects neurons from ischemic cell death, and ameliorates neurobehavioral outcomes after embolic MCA occlusion. [4]

Protocol (from reference)

Kinase Assay:

[1]
  • Fluorescence enzymatic activity assays

    The enzymatic activity of MMP-2, MMP-9 and MMP-7 is monitored with the fluorescence quenched substrate MOCAcPLGLA2pr(Dnp)-AR-NH2. Fluorescence is measure with a PTI spectrofluorometer. The cuvette compartment is thermostated at 25 ℃ 。
Cell Assay:

[5]
  • Cell lines

    HCC cells

  • Concentrations

    0.1 μM

  • Incubation Time

    24 h

  • Method

    Cells were treated with indicated concentration of drug for 24 h.
Animal Study:

[2]
  • Animal Models

    Mouse L-CI.5s T-cell lymphoma model

  • Dosages

    50 mg/kg/d

  • Administration

    i.p.

Customer Product Validation

Data from [Data independently produced by , , Cell Physiol Biochem, 2017, 44(6):2281-2295]

Data from [Data independently produced by , , Mol Nutr Food Res, 2018, doi:10.1002/mnfr.201700969]

Data from [Data independently produced by , , Neuropharmacology, 2017, 118:157-166]

Data from [Data independently produced by , , Exp Cell Res, 2018, 337(1):16-27]

Selleck's SB-3CT has been cited by 28 publications

An MMP-9 exclusive neutralizing antibody attenuates blood-brain barrier breakdown in mice with stroke and reduces stroke patient-derived MMP-9 activity [ Pharmacol Res, 2023, 190:106720] PubMed: 36893823
COL4A3 Mutation Induced Podocyte Apoptosis by Dysregulation of NADPH Oxidase 4 and MMP-2 [ Kidney Int Rep, 2023, 8(9):1864-1874] PubMed: 37705901
Inhibition of MMP-2 and MMP-9 attenuates surgery-induced cognitive impairment in aged mice [ Brain Res Bull, 2023, 204:110810] PubMed: 37939860
Microglial Activation Damages Dopaminergic Neurons through MMP-2/-9-Mediated Increase of Blood-Brain Barrier Permeability in a Parkinson's Disease Mouse Model [ Int J Mol Sci, 2022, 23(5)2793] PubMed: 35269933
MMP2 and MMP9 Activity Is Crucial for Adult Visual Cortex Plasticity in Healthy and Stroke-Affected Mice [ J Neurosci, 2022, 42(1):16-32] PubMed: 34764155
Tucatinib has Selective Activity in HER2-Positive Cancers and Significant Combined Activity with Approved and Novel Breast Cancer-Targeted Therapies [ Cancers (Basel), 2022, 14(7)1630] PubMed: 35406401
MMP2 and MMP9 contribute to lung ischemia-reperfusion injury via promoting pyroptosis in mice [ BMC Pulm Med, 2022, 22(1):230] PubMed: 35705936
Rotenone impairs learning and memory in mice through microglia-mediated blood brain barrier disruption and neuronal apoptosis [ Chemosphere, 2021, S0045-6535(21)03454-8] PubMed: 34822863
GSK-3α/β Activity Negatively Regulates MMP-1/9 Expression to Suppress Mycobacterium tuberculosis Infection [ Front Immunol, 2021, 12:752466] PubMed: 35095838
Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1 [ Genome Med, 2020, 12(1):83] PubMed: 32988398

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.