Home GPCR & G Protein Cannabinoid Receptor antagonist - MmpL3 inhibitor - Transferase inhibitor - Bacterial inhibitor - Acyltransferase inhibitor Rimonabant (SR141716)

Rimonabant (SR141716)

Catalog No.S3021

For research use only.

Rimonabant (SR141716) is a selective antagonist of CB1 with IC50 of 13.6 nM and EC50 of 17.3 nM in hCB1 transfected HEK 293 membrane. Rimonabant is also a dual inhibitor of acyl CoA:cholesterol acyltransferases(ACAT) 1 and 2 and inhibits mycobacterial MmpL3.

Rimonabant (SR141716) Chemical Structure

CAS No. 168273-06-1

Selleck's Rimonabant (SR141716) has been cited by 4 Publications

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Cannabinoid Receptor Signaling Pathways

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Biological Activity

Description Rimonabant (SR141716) is a selective antagonist of CB1 with IC50 of 13.6 nM and EC50 of 17.3 nM in hCB1 transfected HEK 293 membrane. Rimonabant is also a dual inhibitor of acyl CoA:cholesterol acyltransferases(ACAT) 1 and 2 and inhibits mycobacterial MmpL3.
Features Efficacious to induce weight reduction and improvements in cardiometabolic risk factors, however was withdrawn in 2009 due to severe depressive disorder and anxiety.
Targets
ACAT1 [2]
()		 ACAT2 [2]
()		 MmpL3 [7]
()		 hCB1 [1]
(Cell-free assay)		 hCB2 [1]
(Cell-free assay)
13.6 nM 1.64 μM
In vitro

Rimonabant dose-dependently reduces ACAT activity in Raw264.7macrophages with IC50 of 2.9 μM and isolated peritoneal macrophages. Rimonabant inhibits ACATactivity in intact CHO-ACAT1 and CHO-ACAT2 cells and in cell-free assays with approximately equal efficiency with IC50 of 1.5 μM and 2.2 μM for CHO-ACAT1 and CHO-ACAT2, respectively. Consistent with ACAT inhibition, Rimonabant treatment blocks ACAT dependent processes in macrophages, oxysterol-induced apoptosis and acetylated-LDL induced foam cell formation. [2] Rimonabant antagonizes the inhibitory effects of cannabinoid receptor agonists on both mouse vas deferens contractions and adenylyl cyclase activity in rat brain membranes in a concentration-dependent manner. [3] Rimonabant significantly reduces cell growth and induces cell death of human colorectal cancer cells (DLD-1, CaCo-2 and SW620). Rimonabant is able to alter cell cycle distribution in all the cell lines tested. Particularly, Rimonabant produces a G2/M cell cycle arrest in DLD-1 cells without inducing apoptosis or necrosis. [4]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO cells NFjFdVZHfW6ldHnvckBie3OjeR?= MWjBZoltcXS7IITvJIRqe3CuYXPlJHs{UF1vU2KtJFE1OTdzNlGgZolv\GmwZzD0c{BpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuIH3lcYJz[W6nczygT4k:QC57IH7N M3;PTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNE[1OVUzLz5zMES2OVU2OjxxYU6=
CHO M4H1UWZ2dmO2aX;uJIF{e2G7 NXj3PFZSSW62YXfvcol{fGmlIHHjeIl3cXS7IITve4Fz\HNiY3HucoFjcW6xaXSgdoVk\XC2b4KgNUBmgHC{ZYPz[YQh[XNiW{PIYWFz[WOqaXTvcolkKGGlaXSgdoVt\WG|ZTDpckBEUE9iY3XscJMtKEumPUCuNFAzPTF{zszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd|NkK0N{c,OTR5M{[yOFM9N2F-
CHO NFf1e2hHfW6ldHnvckBie3OjeR?= NXTi[oprTGm|cHzhZ4Vu\W62IH;mJGNRNTV3OUSwJIJqdmSrbneg[pJwdSC{ZXPvcYJqdmGwdDDoeY1idiClYX7uZYJqdm:rZDDy[YNmeHSxcjCxJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODJ3zszN M3[4ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2N{O2NlQ{Lz5zNEezOlI1OzxxYU6=
CHO NFvsR5VHfW6ldHnvckBie3OjeR?= NGrUUoZC\m[rbnn0fUB1dyCmaYPwcIFk\SCFUD21OVk1OCCkaX7kbY5oKG[{b32gR4FvdmGkaX7vbYQhemWlZYD0c5IhOiCxZjDoeY1idiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBMcT1zLkW4{txO MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd|NkK0N{c,OTR5M{[yOFM9N2F-
CHO M3rjRWZ2dmO2aX;uJIF{e2G7 M1P2d2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKHKnY3;tZolv[W62IHPhco5i[mmwb3nkJJJm[2WydH;yJJR6eGViMTDlfJBz\XO|ZXSgbY4hS2irbnXz[UBJ[W2|dHXyJG93[XK7IDjDTG8qKGOnbHzzMEBKSzVyPUCuNFA3|ryP NEjzU3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexN|QxOyd-MUW3NVM1ODN:L3G+
CHO Mn2wSpVv[3Srb36gZZN{[Xl? MkTNTY4hfmm2cn:gZ4FvdmGkaX7vbYQhemWlZYD0c5IhOSCjboTh[49vcXOvIH;mJHs{UF2jcnHjbIlld26rYzDhZ4llKHKnbHXhd4Uh[nliQ1jPJINmdGy|LDDL[F0xNjByMkWxNu69VQ>? M4fweFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFI5Lz5zNUe3NVQzQDxxYU6=
CHO NUnS[|NZTnWwY4Tpc44h[XO|YYm= NVPybpdtUW5idnn0do8h\Gm|cHzhZ4Vu\W62IH;mJGNRNTV3OUSwJIJqdmSrbnegeI8hcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOsJGtqRTBwMEK1{txO NH\XWnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQzQCd-MUW3O|E1Ojh:L3G+
CHO NVTzZlVsTnWwY4Tpc44h[XO|YYm= MoTSTY4hfmm2cn:g[Il{eGyjY3Xt[Y51KG:oIFPQMVU2QTRyIHLpcoRqdmdidH:gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVEvPTkQvF2= NGjUWIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQzQCd-MUW3O|E1Ojh:L3G+
CHO MojmSpVv[3Srb36gZZN{[Xl? Ml;LTY5pcWKrdHnvckBw\iCdM1jdV3IuOTRzLEexOmEh[mmwZHnu[{B1dyCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{whU2l;MD6wNFU1|ryP MmHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6MEG4OFAoRjF3OECxPFQxRC:jPh?=
CHO MVnGeY5kfGmxbjDhd5NigQ>? MkXnRY51[WexbnnzeIlkKGGldHn2bZR6KGGpYXnud5Qh[2GwbnHibY5wcWRicnXj[ZB1d3JiMTDt[YF{fXKnZDDifUBEWC13NUm0NEBqdmS3Y3XkJIFz[WOqbn;p[EBi[2mmIILlcIVie2ViaX6gR2hQKGOnbHzzMEBM\D1yLkCwNlUyOs7:TR?= NIDVXZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkG0NFAyOCd-MU[xOFAxOTB:L3G+
CHO M4XLO2Z2dmO2aX;uJIF{e2G7 NH;CO|RFcXOybHHj[Y1mdnRib3[gd5Bm[2moaXOgR3AuPTV7NECgZolv\GmwZzDpckBEUE9iY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iY3HucoFjcW6xaXSgdoVk\XC2b4KgNUwhU2l;MD6wNlXPxE1? NFWxTpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkG0NFAyOCd-MU[xOFAxOTB:L3G+
CHO M3rFbWZ2dmO2aX;uJIF{e2G7 NHy2cZZFcXOybHHj[Y1mdnRib3[gd5Bm[2moaXOgR3AuPTV7NECgZolv\GmwZzDpckBEUE9iY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iY3HucoFjcW6xaXSgdoVk\XC2b4KgNkwhU2l;MT61PO69VQ>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjF2MECxNEc,OTZzNECwNVA9N2F-
CHOK1 MWrGeY5kfGmxbjDhd5NigQ>? NUDSUVRmTnWwY4Tpc45idCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4KgeJJidnOoZXP0[YQhcW5iQ1jPT|Eh[2WubIOgZpkhYzN3U1fUVH1o[W2vYWOgZZN{[XluIFvpQVAvODBzzszN NYT6[lB5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOlMzQDNpPkG2NlY{Ojh|PD;hQi=>
HEK293 M{KzWWZ2dmO2aX;uJIF{e2G7 M4PsdGRqe3CuYXPlcYVvfCCxZjDbN2heW1JvMUSxO|E3KG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJJRz[W6|ZnXjeIVlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNFIy|ryP MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ4M{K4N{c,OTZ{NkOyPFM9N2F-
CHO M4rkUWZ2dmO2aX;uJIF{e2G7 M4i4N2Rqe3CuYXPlcYVvfCCxZjDbN2heW1JzNEG3NVZCKG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODB3NN88US=> MnXoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{N{m4NFkoRjF4Mke5PFA6RC:jPh?=
HEK293 MlH1SpVv[3Srb36gZZN{[Xl? NGPG[XhFcXOybHHj[Y1mdnRib3[gX|NJZVOULUG0NVcyPiCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBM\D1yLkCwNVjPxE1? NYHkRVJ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OTJpPkG3NFA1PzF{PD;hQi=>
HEK293 MXzGeY5kfGmxbjDhd5NigQ>? MULEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB5Md88US=> NX[ySHoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OTJpPkG3NFA1PzF{PD;hQi=>
HEK293 NIDKbFBHfW6ldHnvckBie3OjeR?= M1rlSGRqe3CuYXPlcYVvfCCxZjDbN2heX0mQLUW1NlEzNTJiZoLvcUBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNVjPxE1? M3ywelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC0O|EzLz5zN{CwOFcyOjxxYU6=
CHO MoXzSpVv[3Srb36gZZN{[Xl? NYey[YhbUW6qaXLpeIlwdiCxZjDbN2heS1BvNUW5OFAh[mmwZHnu[{B1dyCqdX3hckBz\WOxbXLpcoFvfCCFQkGgdoVk\XC2b4KgbY4hS0iRIHPlcIx{NCCLQ{WwQVAvODB4Md88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF6MUGzPEc,OTdzOEGxN|g9N2F-
CHO MVTGeY5kfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKFt|SG3DVE02PTl2MDDibY5lcW6pIITvJIh2dWGwIILlZ49u[mmwYX70JGNDOiC{ZXPldJRweiCrbjDDTG8h[2WubIOsJGlEPTB;MD62{txO MlPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxN|goRjF5MUixNVM5RC:jPh?=
CHO MXHGeY5kfGmxbjDhd5NigQ>? NHjqT3NFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOsJGlEPTB;MD6wNFYy|ryP NXfXRmVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVMyODlpPkG3Nlk{OTB7PD;hQi=>
CHO M1fXemZ2dmO2aX;uJIF{e2G7 NEXjVpVFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOsJGlEPTB;MD62NFM{|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ7M{GwPUc,OTd{OUOxNFk9N2F-
HEK293 M4PkNGZ2dmO2aX;uJIF{e2G7 MlvCSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMEGx{txO NGr4eGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{O4N|E5OCd-MUezPFMyQDB:L3G+
HEK293 MX\GeY5kfGmxbjDhd5NigQ>? NGLucHNFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMtKEurPUCuNFE3|ryP MkDzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NEKzNFcoRjF5OUSyN|A4RC:jPh?=
HEK293 M1TufGZ2dmO2aX;uJIF{e2G7 M3[2TGRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MT62OO69VQ>? MlT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7NEKzNFcoRjF5OUSyN|A4RC:jPh?=
HEK293 M4PE[WZ2dmO2aX;uJIF{e2G7 MlntSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkC0O:69VQ>? NHzHZWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3PVI3OSd-MUe5O|kzPjF:L3G+
CHOK1 M3GwPGZ2dmO2aX;uJIF{e2G7 NWCxWW93TGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJ{IH\yc40hcHWvYX6gdoVkd22kaX7hcpQhS0J{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE:NMTDj[YxteyxiS3m9NU46Qc7:TR?= M2K2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe5NlYyLz5zN{m3PVI3OTxxYU6=
HEK293 NFnucHpHfW6ldHnvckBie3OjeR?= MVvEbZNxdGGlZX3lcpQhd2ZicnHkbY9tcWejbnSg[pJwdSCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyxiS3m9NE4xODF6zszN NGP6VlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4N|U3OCd-MUiwPFM2PjB:L3G+
HEK293 EBNA NGnWUIZHfW6ldHnvckBie3OjeR?= MnHiTY53\XK|ZTDh[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KEWETlGgZ4VtdHNiYomgX|M2W12JVGDnZY1u[VNiaX7jc5Jxd3KjdHnvckBie3OjeTygSWM2OD1yLkCwOO69VQ>? M3jxSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEizOVYxLz5zOEC4N|U3ODxxYU6=
HEK293 MWHGeY5kfGmxbjDhd5NigQ>? M4jyemRqe3CuYXPlcYVvfCCxZjDyZYRqd2yrZ3Hu[EBnem:vIHj1cYFvKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDLbV0xNjV3NN88US=> NFjwVnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC4N|U3OCd-MUiwPFM2PjB:L3G+
CHOK1 MX7GeY5kfGmxbjDhd5NigQ>? NE\0[ZNCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRS{GgZ4VtdHNiYomgcJVkcW[ncnHz[UBie3OjeTygTWM2OD1yLkGy{txO MnfmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEO3NVEoRjF6MkSzO|EyRC:jPh?=
CHOK1 NFi1dG1HfW6ldHnvckBie3OjeR?= NFrkWY1FcXOybHHj[Y1mdnRib3[gX|NJZVeLTj21OVIyOi1{IH\yc40hcHWvYX6gR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG9MOSClZXzsd{whUUN3ME2xMlc3|ryP MmnFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEO3NVEoRjF6MkSzO|EyRC:jPh?=
HEK Mn;1SpVv[3Srb36gZZN{[Xl? Mnn5SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLJINmdGy|LDDLbV0xNjBzMt88US=> NFP5RnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK5N|kxQCd-MUiyPVM6ODh:L3G+
HEK MmXPSpVv[3Srb36gZZN{[Xl? M1zOfWRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC55Od88US=> NX:yW45GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyPVM6ODhpPkG4Nlk{QTB6PD;hQi=>
HEK293 M{\6dGZ2dmO2aX;uJIF{e2G7 Mkf2SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMWKg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMEdOwG0> NV;WcpFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizN|U6PzZpPkG4N|M2QTd4PD;hQi=>
CHO MWXGeY5kfGmxbjDhd5NigQ>? M4LJ[GRqe3CuYXPlcYVvfCCxZjDbN2heX0mQLUW1NlEzNTJiZoLvcUBpfW2jbjDjZY5v[WKrbn;p[EBESjJicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{whUUN3ME2xMlc3|ryP NYjnNm9pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizN|cxQTZpPkG4N|M4ODl4PD;hQi=>
CHO NFTBSGRHfW6ldHnvckBie3OjeR?= NX3Rb3JKTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDLbV0xNjB{Nd88US=> NWizWIRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOFI1ODNpPkG4N|QzPDB|PD;hQi=>
CHO NGLySWVHfW6ldHnvckBie3OjeR?= NX\4[YJUTGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJvMjDmdo9uKGi3bXHuJGNDOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBMcT1zLkW4{txO NF;3TXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO0NlQxOyd-MUizOFI1ODN:L3G+
HEK293 NUDxcIZCTnWwY4Tpc44h[XO|YYm= MX7EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB{NN88US=> NXTPc4tRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOlM{PTJpPkG4N|Y{OzV{PD;hQi=>
CHO NIjFdo9HfW6ldHnvckBie3OjeR?= MmDuSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlU3|ryP M2DjUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{[zN|UzLz5zOEO2N|M2OjxxYU6=
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SF9 MmHVSpVv[3Srb36gZZN{[Xl? NUDnbGlUSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKFOIOTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEOSLUW1PVQxNXO2aX31cIF1\WRiR2TQ[4FudWGVIHLpcoRqdmduIFvpQVAvODByNEROwG0> NIW1fIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES0PFM1OCd-MUi0OFg{PDB:L3G+
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HEK293 NF\veWFHfW6ldHnvckBie3OjeR?= M4rkS2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\m:{bTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4k:OC5yNN88US=> M{HBe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGxNVU4Lz5zOEWxNVE2PzxxYU6=
CHO-K1 NHWyS4RHfW6ldHnvckBie3OjeR?= NVXFdJByTGm|cHzhZ4Vu\W62IH;mJHs{UF2VUkG0NVcyPiCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPMWsyKGOnbHzzMEBMcT1yLkCwNVE5|ryP NEXEenc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEWxNlkxOSd-MUi1NVI6ODF:L3G+
HEK293 MoPUSpVv[3Srb36gZZN{[Xl? M3vDTGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFUD21OVk1OC2|dHnteYxifGWmIGuzOXNeT1SSZ3HtcYFUKGKrbnTpcoctKEumPUCuNFAzPTgQvF2= MmDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MUK5NFEoRjF6NUGyPVAyRC:jPh?=
HEK293 NWfp[W1XTnWwY4Tpc44h[XO|YYm= MXXEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB4MUlOwG0> M1;SXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVAyLz5zOEWxNlkxOTxxYU6=
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HEK293 M4i3bGZ2dmO2aX;uJIF{e2G7 NHnUSoJFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqRTBwMEGy{txO NXvjcmh2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2PFAzPzZpPkG4OlgxOjd4PD;hQi=>
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HEK293 NYDuVVBwTnWwY4Tpc44h[XO|YYm= NIHkOnBFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGlEPTB;MD6wNVXPxE1? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDdzMki1Okc,OTh5MUK4OVY9N2F-
HEK293 Ml74SpVv[3Srb36gZZN{[Xl? NULJb|M2SW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFX1MWdVWCCkaX7kbY5oNCCHQ{WwQVAvODF6Mt88US=> Mly3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5MUK4OVYoRjF6N{GyPFU3RC:jPh?=
HEK293 MoLjSpVv[3Srb36gZZN{[Xl? MX3EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0J{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVA:OS57M{m4{txO NGnXblk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEexNlg2Pid-MUi3NVI5PTZ:L3G+
HEK293 MmfrSpVv[3Srb36gZZN{[Xl? M13EV2lvfmW{c3WgZYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDbN|VUZWejbX3hS3RRKGKrbnTpcochemWuYYTpeoUhfG9iY3;ueJJwdA>? Mn7TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5MUK4OVYoRjF6N{GyPFU3RC:jPh?=
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CHOK1 NUf5bFBWTnWwY4Tpc44h[XO|YYm= MXTBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJINidm6jYnnuc4llKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRS{GgZ4VtdHNiYYPz[ZN{\WRiYYOgZ2FOWCCjY4Tpeol1gSxiRVO1NF0xNjJ2zszN NXHsO|RVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzNlg3QDNpPkG5N|I5Pjh|PD;hQi=>
CHOK1 NIPxdlFHfW6ldHnvckBie3OjeR?= MUDBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJINidm6jYnnuc4llKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRS{GgZ4VtdHNiYYPz[ZN{\WRiYYOgZ2FOWCCjY4Tpeol1gSxiRVO1NF0{OS5{Md88US=> NELO[Vc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOyPFY5Oyd-MUmzNlg3QDN:L3G+
HEK293 MlTLSpVv[3Srb36gZZN{[Xl? M4TUSmRqe3CuYXPlcYVvfCCxZjDbN2heW1JzNEG3NVYh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNFE6QTYQvF2= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN|OEO1Okc,OTl|M{izOVY9N2F-
HEK293 MlXuSpVv[3Srb36gZZN{[Xl? MofOSIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOkBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkO5PFEy|ryP NV;CcY5jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzN|g{PTZpPkG5N|M5OzV4PD;hQi=>
CHOK1 MnrwSpVv[3Srb36gZZN{[Xl? MlXZNVAhdWmwcx?= MnfqRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJV0tzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1BvNUW5OFAucW6mdXPl[EBz\XOyb37z[UBi\nSncjCxNEBucW6|IHL5JGdVWGejbX3hX|M2W11iYnnu[Ilv\yCjc4PhfUwhU2l;MD6wNFE3|ryP NXzDN|JyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzOVEyOTNpPkG5N|UyOTF|PD;hQi=>
HEK293 NEPCNZdHfW6ldHnvckBie3OjeR?= NYeyNVhITGm|cHzhZ4Vu\W62IH;mJHs{UF2{aX3vcoFj[W62IH\yc40hcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwMECxPe69VQ>? M1zvNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUKwOVczLz5zOUWyNFU4OjxxYU6=
HEK293 NYj6VmxmTnWwY4Tpc44h[XO|YYm= MV\EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KxVkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBKSzVyPUCuNFE{Os7:TR?= M1\wXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUOwOlk4Lz5zOUWzNFY6PzxxYU6=
HEK293 MnrBSpVv[3Srb36gZZN{[Xl? NX:zeZVLUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEOEMWKg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQMVU2QTRyLYP0bY12dGG2ZXSgSZUuT1SSIHLpcoRqdmduIFXDOVA:OC5yMUW3{txO Ml;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{C2PVcoRjF7NUOwOlk4RC:jPh?=
HEK293 MVnGeY5kfGmxbjDhd5NigQ>? NFHEOmVFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzWiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{NCCLQ{WwQVEvPjNzMd88US=> MmjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{C2PVcoRjF7NUOwOlk4RC:jPh?=
HEK293 Mly4SpVv[3Srb36gZZN{[Xl? MUexNEB2VQ>? MXnJcpRzcW6|aXOgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhS0JzUjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJGV2NUeWUDDibY5lcW6pIHH0JFExKHWPIILlcIF1cX[nIITvJIJie2GuIHzleoVt M{KxXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUOwOlk4Lz5zOUWzNFY6PzxxYU6=
HEK M2HDfWZ2dmO2aX;uJIF{e2G7 M1zSb2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC5yMUNOwG0> MlrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OUW1PVYoRjF7NUm1OVk3RC:jPh?=
HEK M3rWNmZ2dmO2aX;uJIF{e2G7 M1\2O2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC55Od88US=> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV7NUW5Okc,OTl3OUW1PVY9N2F-
CHO-K1 MVfGeY5kfGmxbjDhd5NigQ>? MlviRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JidILhcpNn\WO2ZXSgbY4hS0iRLVuxZ4VtdHNiYomgS3RR\2GvbXHbN|VUZSCkaX7kbY5oKGG|c3H5MEBMcT1yLkCwNVbPxE1? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTZ6M{mxPEc,OTl4OEO5NVg9N2F-
CHO MUfGeY5kfGmxbjDhd5NigQ>? NEj4cIhDcW6maX7nJIFn\mmwaYT5JJRwKGi3bXHuJGNDOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkC5NlXPxE1? NYTPWYlMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4OVA1PzNpPkG5PFUxPDd|PD;hQi=>
CHOK1 M3zSV2Z2dmO2aX;uJIF{e2G7 MVTEbZNxdGGlZX3lcpQhd2ZiW{PIYXdKVi13NUKxNlIh\nKxbTDoeY1idiCFQkKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|LDDJR|UxRTFwN{dOwG0> MmXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NUC0O|MoRjF7OEWwOFc{RC:jPh?=
CHO-K1 MlnTSpVv[3Srb36gZZN{[Xl? MUDBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRLVuxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2CtOVU6PDBvc4TpcZVt[XSnZDDHWHBo[W2vYWOgZolv\GmwZzygTWM2OD1yLkCwOFXPxE1? M{P1VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OUW0PVc5Lz5zOUm1OFk4QDxxYU6=
CHO-K1 M3[5bWZ2dmO2aX;uJIF{e2G7 NF\HSY9Kdn[ncoPlJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMh[nliR2TQ[4FudWGVIHLpcoRqdmdiYYPzZZktKEmFNUC9NE4xODJ7zszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODBzNU[0O{c,OjByMUW2OFc9N2F-
CHO-K1 NI\tWIxHfW6ldHnvckBie3OjeR?= MUnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1BvNUW5OFAucW6mdXPl[EBIXFCpYX3tZXMh[mmwZHnu[{whUUN3ME2wMlAxPDYQvF2= NX\tVlJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwNVU3PDdpPkKwNFE2PjR5PD;hQi=>
COS7 NXjENZVETnWwY4Tpc44h[XO|YYm= NXnsdFAzTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczygTWM2OD1yLkCwOVHPxE1? NHvkNIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECxOVY1Pyd-MkCwNVU3PDd:L3G+
CHO M1;FeWZ2dmO2aX;uJIF{e2G7 NF61[npKdn[ncoPlJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUCuNVA5|ryP NVS1b3FsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwOFU{OzdpPkKwNFQ2OzN5PD;hQi=>
CHO NYrkfJV4TnWwY4Tpc44h[XO|YYm= MXm2NEBucW6| NHf2TmFKdn[ncoPlJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZ4FvdmGkaX7vbYQhS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBnd3K|a3;sbY4ue3SrbYXsZZRm\CClQV3QJIFk[3WvdXzheIlwdiCjZoTldkA3OCCvaX7zMEBGSzVyPUCuNFAxOTIQvF2= Mn;3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByNEe3O|koRjJyMES3O|c6RC:jPh?=
CHO MXnGeY5kfGmxbjDhd5NigQ>? MoPwN{BpenN? MV7EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiY3HucoFjcW6xaXSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZYZ1\XJiMzDodpMh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwMECwO|TPxE1? NETTdog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC0O|c4QSd-MkCwOFc4Pzl:L3G+
CHO NV7yfZJETnWwY4Tpc44h[XO|YYm= MWKxNEBucW6| Mn;SRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBk[W6wYXLpco9q\CCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyClb3X4dJJme3OrbnegS4FteGijMUWvNVYh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBEWC13NUm0NE1qdmS3Y3XkJGNiOiticnXs[YF{\SCjZoTldkAyOCCvaX7zJIJ6KG2rY4LvJJBt[XSnIILlZYRmeixiSVO1NF0xNjByM{NOwG0> M4jvUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMES3O|c6Lz5{MEC0O|c4QTxxYU6=
CHO Mm\iSpVv[3Srb36gZZN{[Xl? MlrxN{BpenN? NHjNZnZFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgZ4FvdmGkaX7vbYQhS0J{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[W[2ZYKgN{BpenNiYomgcIlyfWmmIIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurPUCuNVI3|ryP M2fOS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMES3O|c6Lz5{MEC0O|c4QTxxYU6=
CHO NUW1dodOTnWwY4Tpc44h[XO|YYm= MYnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gR3AuPTV7NECtbY5lfWOnZDDbN2he[XKjY3jp[I9vcWNiYXPp[EBz\WynYYPlMEBM\D1yLkCwNlUyOs7:TR?= NYjPW2ZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOlMyOzJpPkKwN|Y{OTN{PD;hQi=>
CHO MUjGeY5kfGmxbjDhd5NigQ>? MUDEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUCuNFI2|ryP M4LYXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{[zNVMzLz5{MEO2N|E{OjxxYU6=
CHO MkLESpVv[3Srb36gZZN{[Xl? MUDEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0J{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUGuOVjPxE1? M2DkWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{[zNVMzLz5{MEO2N|E{OjxxYU6=
CHO-K1 M37xcGZ2dmO2aX;uJIF{e2G7 MU[xJIhz NUnPe3VtTGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJvMjDmdo9uKGi3bXHuJGNDOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGGodHXyJFEhcHJiYomgcIlyfWmmIIPjbY51cWyuYYTpc44h[2:3boTpcoctKEmFNUC9NU44Ps7:TR?= NY[1VZh1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2O|M4OjlpPkKwOlc{PzJ7PD;hQi=>
HEK NEjkT3ZHfW6ldHnvckBie3OjeR?= MlrtSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNIHPlcIx{NCCNaU2wMlAyOs7:TR?= NVL1XWl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC3NVg1QTJpPkKwO|E5PDl{PD;hQi=>
HEK NVTvcmo3TnWwY4Tpc44h[XO|YYm= NGrZdXZFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVugZ4VtdHNuIFvpQVAvPzoQvF2= NU\EdoR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC3NVg1QTJpPkKwO|E5PDl{PD;hQi=>
CHO NIDIZYhHfW6ldHnvckBie3OjeR?= M4rB[2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZ48u\XiycnXzd4lv\yCJYXzwbIEyPiCycn;0[YlvKGG|c3Xzd4VlKGG|IH3vZoltcXqjdHnvckBw\iCrboTyZYNmdGy3bHHyJINidGOrdX2sJGtmRTBwMECxNe69VQ>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh2NUm1PUc,OjB6NEW5OVk9N2F-
HEK293 MUHGeY5kfGmxbjDhd5NigQ>? MnjBSIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOkBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDLbV0xNjByMUG4{txO NEjWXVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi0OVk2QSd-MkC4OFU6PTl:L3G+
HEK293 NXnI[Gs1TnWwY4Tpc44h[XO|YYm= MmHuSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB4MUlOwG0> NV7FW4RlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OFU6PTlpPkKwPFQ2QTV7PD;hQi=>
CHOK1 NF\2cYhHfW6ldHnvckBie3OjeR?= NWfudmVXTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUEW1PVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF;LNUBk\WyuczygT4k:OC5|MUROwG0> M{S5fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOES1PVU6Lz5{MEi0OVk2QTxxYU6=
HEK MWHGeY5kfGmxbjDhd5NigQ>? M3;HXGRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUWsPVQxKG[{b32gdoVkd22kaX7hcpQhcHWvYX6gR2IyKHKnY3XweI9zKHS{YX7z[oVkfGWmIHnuJGhGUyClZXzsd{whU2l;MD6wNVLPxE1? NYfHVYVbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC5OFMzQTBpPkKwPVQ{OjlyPD;hQi=>
HEK NEnaWYJHfW6ldHnvckBie3OjeR?= MljoSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NTy5OFAh\nKxbTDy[YNwdWKrbnHueEBpfW2jbjDDRlIhemWlZYD0c5IhfHKjboPm[YN1\WRiaX6gTGVMKGOnbHzzMEBMcT1yLke5{txO M4rGVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUSzNlkxLz5{MEm0N|I6ODxxYU6=
CHO NYroXFRrTnWwY4Tpc44h[XO|YYm= M3rLUGRqe3CuYXPlcYVvfCCxZjDbN2heW1JzNEG3NVZCKG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvODByOUOzN:69VQ>? NYDkVVBWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|Q5QTJpPkKxN|M1QDl{PD;hQi=>
Sf9 NHXGeHRHfW6ldHnvckBie3OjeR?= Mlf4SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCVZkmgZ4VtdHNuIFvpQVAvQDVzMUVOwG0> NHHje3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzOFg6Oid-MkGzN|Q5QTJ:L3G+
CHO M3X6cGZ2dmO2aX;uJIF{e2G7 NFLaRmpCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFPCNUBz\WOncITvdkB1emGwc3\lZ5Rm\CCrbjDDTG8h[2WubIOg[ZhxemW|c3nu[{BieG:jZYH1c5JqdiCjczDhJJJmeG:{dHXyJIZweiCJLYDyc5RmcW5vY3;1dIxm\CC{ZXPldJRwei2vZXTpZZRm\CClYXzjbZVuKHOrZ37hcIlv\yCkeTDibY9tfW2rbnXzZ4Vv[2ViYYPzZZktKEmFNUC9NE4xODUQvF2= NVvYdmlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzO|Y2QDhpPkKxN|c3PTh6PD;hQi=>
HEK293 MXrGeY5kfGmxbjDhd5NigQ>? MYDOc45kd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJG1TWDFvbXXkbYF1\WRiRUKtNVdj\XSjRzD0doFve3CxcoSgbY4hcHWvYX6gUXJROSCneIDy[ZN{cW6pIFjFT|I6OyClZXzsd{BjgSCGaYjvckBxdG:2LDDLbV0yNjUQvF2= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 Mo\XSpVv[3Srb36gZZN{[Xl? NWD0XXQ2Vm:wY3;tdIV1cXSrdnWgbY5pcWKrdHnvckBw\iCPUmC0MY1m\GmjdHXkJGUzNTF5YnX0ZWchfHKjboPwc5J1KGmwIHj1cYFvKE2UUESg[ZhxemW|c3nu[{BJTUt{OUOgZ4VtdHNiYomgSIl5d25icHzveEwhU2l;NN88US=> M{Ww[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGxPVQ2Lz5{MUWxNVk1PTxxYU6=
HEK293 M2C4bGZ2dmO2aX;uJIF{e2G7 M3roPGlv\HWldHnvckBw\iCPUmCyMY1m\GmjdHXkJGUzNTF5YnX0ZWchfHKjboPwc5J1KGmwIHj1cYFvKE2UUEKg[ZhxemW|c3nu[{BJTUt{OUOgZ4VtdHNiaX6gdJJme2WwY3Wgc4YhOiC3TTDFNk0yP2KndHHH MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 MWHGeY5kfGmxbjDhd5NigQ>? NY[5N2tIUW6qaXLpeIlwdiCxZjDNVnA{NW2nZHnheIVlKEV{LUG3ZoV1[UdidILhcpNxd3K2IHnuJIh2dWGwIF3SVFMh\XiycnXzd4lv\yCKRVuyPVMh[2WubIOgbY4heHKnc3XuZ4Uhd2ZiMUWgeW0hTTJvMUfi[ZRiTw>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 M13UPGZ2dmO2aX;uJIF{e2G7 NIPjephKdmirYnn0bY9vKG:oIF3SVFEudWWmaXH0[YQhTTJvMUfi[ZRiTyC2cnHud5BwenRiaX6gbJVu[W5iTWLQNUBmgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczDpckBxemW|ZX7j[UBw\iByLk[geI8hPSC3TTDFNk0yP2KndHHH MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 MVPGeY5kfGmxbjDhd5NigQ>? NGPtTotKdmirYnn0bY9vKG:oIF3SVFQudWWmaXH0[YQhTTJvMUfi[ZRiTyC2cnHud5BwenRiaX6gbJVu[W5iTWLQOEBmgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczDpckBxemW|ZX7j[UBw\iByLk[geI8hPSC3TTDFNk0yP2KndHHH M1rOT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUGxPVQ2Lz5{MUWxNVk1PTxxYU6=
HEK MnLZSpVv[3Srb36gZZN{[Xl? M3[1N2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC5yMUNOwG0> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyMkS5PEc,OjF5MEK0PVg9N2F-
HEK MXzGeY5kfGmxbjDhd5NigQ>? M2raeWRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC55Od88US=> Ml[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEK0PVgoRjJzN{CyOFk5RC:jPh?=
CHO MWrGeY5kfGmxbjDhd5NigQ>? MXSxJIhz NYHiepRHTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUEW1PVQxKG[{b32gbJVu[W5iY3HucoFjcW6xaXSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZQheEhiNz60JIFnfGW{IEGgbJIh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGlEPTB;MT65PO69VQ>? M3nXZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{SxPFM2Lz5{MUe0NVg{PTxxYU6=
HEK293 NHXMb21HfW6ldHnvckBie3OjeR?= NV:yU2pWPSCvaX7z NGHOfZpKdmirYnn0bY9vKG:oIHj1cYFvKEWURzDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZicH;0ZZN{cXWvIHPoZY5v\WxiY4XydoVvfCCjZoTldkA2KG2rboOgZpkheGG2Y3igZ4xidXBiYYPzZZktKEmFNUC9Nk44Qc7:TR?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd2MUizOUc,OjF5NEG4N|U9N2F-
CHO MnfwSpVv[3Srb36gZZN{[Xl? MYq5NEBucW6| NEfPWXlFcXOybHHj[Y1mdnRib3[gX|NJZUKPUz23NlU2OTliZoLvcUBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDh[pRmeiB7MDDtbY5{KGK7IIPjbY51cWyuYYTpc44h[2:3boTpcoctKEurPUCuNFAxPzQQvF2= MoS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkK1O|UoRjJzOU[yOVc2RC:jPh?=
CHO-K1 M2LYfWZ2dmO2aX;uJIF{e2G7 M2iySGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9vS{GgZ4VtdHNiY3:t[ZhxemW|c3nu[{BI[WyyaHHxNVYh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBEWDV3OUSwMYlv\HWlZXSgbY51emGlZXzseYxieiClYXzjbZVuKG2xYnnsbZpifGmxbjDifUBndHWxcn;t[ZRzgSxiS3W9NE4xODFzzszN NWnUUG9YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzO|I5OzVpPkKyN|czQDN3PD;hQi=>
CHO-K1 MYfGeY5kfGmxbjDhd5NigQ>? Mk\wSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9vS{GgZ4VtdHNuIFvpQVAvODB4Mt88US=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN5MkizOUc,OjJ|N{K4N|U9N2F-
CHO-K1 NGW0cIxHfW6ldHnvckBie3OjeR?= NX\VWZFlTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUEW1PVQxKG[{b32gR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMtKEurPUCuN|E{|ryP M3\u[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{eyPFM2Lz5{MkO3Nlg{PTxxYU6=
HEK MmfGSpVv[3Srb36gZZN{[Xl? M1TNcWRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUWsPVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKHS{YX7z[oVkfGWmIHnuJGhGUyClZXzsd{whU2l;MD6wNVLPxE1? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2OES1O{c,OjJ3NEi0OVc9N2F-
HEK MWnGeY5kfGmxbjDhd5NigQ>? NVy0e29wTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OUw6PDBiZoLvcUBpfW2jbjDy[YNwdWKrbnHueEBESjJicnXj[ZB1d3JidILhcpNn\WO2ZXSgbY4hUEWNIHPlcIx{NCCNaU2wMlc6|ryP NUG2UnZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OFg1PTdpPkKyOVQ5PDV5PD;hQi=>
CHO NY\wc49STnWwY4Tpc44h[XO|YYm= MmPFNkBpenN? Mn\GSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3LEm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGGodHXyJFIhcHK|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70[ZItKEurPUCuNFEzPs7:TR?= M2mwclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUG2O|A4Lz5{MkmxOlcxPzxxYU6=
CHO MnTXSpVv[3Srb36gZZN{[Xl? Ml7FNkBpenN? NF7CWZBFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUWsPVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRmeixiS3m9NE46|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjlzNkewO{c,OjJ7MU[3NFc9N2F-
CHO MoLHSpVv[3Srb36gZZN{[Xl? MWnJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYXfhbY5{fCCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKGi3bXHuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCnZn\lZ5Qhd25iZn;yd4twdGmwLYP0bY12dGG2ZXSgZ2FOWCCuZY\lcEwhUUN3ME2wMlA2Oc7:TR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl3OUK0PUc,OjJ7NUmyOFk9N2F-
CHO MmHlSpVv[3Srb36gZZN{[Xl? M3PM[FEhcHJ? MmO5SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMhcW6ldXLheIVlKG[xcjCxJIhzNCCNaU2wMlAyODcQvF2= NInpPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3NlM{QSd-MkOwO|I{Ozl:L3G+
CHO NWnQVJRsTnWwY4Tpc44h[XO|YYm= M{TQeF4hOC5zIH7N Ml7QNUBpeg>? NIC1NFBKdn[ncoPlJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gdoVkd22kaX7hcpQhS0JzIILlZ4VxfG:{IITyZY5{\mWldHXkJIlvKEOKTzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gc4YhYzN3U23HWHBo[W2vYWOgZolv\GmwZzDheEA,KDBwMTDuUUBi\nSncjCxJIhzKGK7IHzpdZVq\CC|Y3nueIltdGG2aX;uJJNx\WO2cn;t[ZRzgQ>? M2nGWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{W3N|A4Lz5{M{O1O|MxPzxxYU6=
N1E-115 NF7TO2lHfW6ldHnvckBie3OjeR?= NVGzUHo6OSC3TR?= M2\3NVUhdWmwcx?= NXvjNlNnUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KEOEMTDy[YNmeHSxcjDpckBud3W|ZTDONWUuOTF3IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhX0mQIEW1MFIyOi1{LXnu[JVk\WRiRWLLNU8zKHCqb4PwbI9zgWyjdHnvckBifCBzIIXNJJRz\WG2ZXSgOUBucW6|IIDybY9zKHSxIGfJUkA2PSx{MUKtNkBkcGGubHXu[4Uh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MmnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NUezNFcoRjJ|M{W3N|A4RC:jPh?=
CHO NWLyNGJjTnWwY4Tpc44h[XO|YYm= NWe0[3VkSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCJUGKxPEB1emGwc3\lZ5Rm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMh\GWudHG5MXRJSy2rbnT1Z4VlKGKndHGtZZJz\XO2aX6gdoVkenWrdH3lcpQhcW6ldXLheIVlKDZyIH3pcpMheHKrb4KgeI8h\GWudHG5MXRJSyCjZHTpeIlwdiCkeTDi[ZRiNWG{cnXzeIlvKHS{YX7zcI9k[XSrb36gZZN{[XluIFnDOVA:OTBwMd88US=> NVXxW3VxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2O|k6PTVpPkKzOlc6QTV3PD;hQi=>
HEK MmLOSpVv[3Srb36gZZN{[Xl? NH3zcYQyKGi{ MXXBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCxdnXy[ZhxemW|c3XkJIlvKEiHSzDj[YxteyCjc4Pld5Nm\CCjczDbN|VUZUeWUHfhcY1iKGKrbnTpcoch[W[2ZYKgNUBpeiCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpd{whTUN3ME2wMlAxOTYQvF2= Mo[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{W1O|IoRjJ2MUe1OVczRC:jPh?=
CHOK1 MkPKSpVv[3Srb36gZZN{[Xl? MXSxJIhz MkK2SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE:NMTDj[YxteyCjZoTldkAyKGi{IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70bY5oKGGwYXz5d4l{NCCNaU2wMlAxPToQvF2= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5NUW3Nkc,OjRzN{W1O|I9N2F-
CHO MYHGeY5kfGmxbjDhd5NigQ>? Mne5OFUhdWmwcx?= M1zVd2FvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IIP0ZYJtgSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJINwNWW6cILld5NqdmdiY3:t[ZhxemW|c3nu[{BI[TF3L{G2JIF{e2W|c3XkJIF{KGOjbHPpeY0h[3W{cnXueEBi\nSncjC0OUBucW6|IHL5JIZtfW9vNDDBUUBie3OjeTygTWM2OD1yLkCxN:69VQ>? NXjYUHZPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OFU{OTBpPkK0OFQ2OzFyPD;hQi=>
CHO NIrpRXZHfW6ldHnvckBie3OjeR?= M{W3ZlQ2KG2rboO= NG\m[5hCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFPCNkBz\WOncITvdkApfW6tbn;3ckBwemmpaX6pJJN1[WKueTDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGOxLXX4dJJme3OrbnegZ48u\XiycnXzd4lv\yCJYUG1M|E3KGG|c3Xzd4VlKGG|IHPhcINqfW1iY4XydoVvfCCjZoTldkA1PSCvaX7zJIJ6KG[udX:tOEBCVSCjc4PhfUwhUUN3ME25MljPxE1? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2NUOxNEc,OjR2NEWzNVA9N2F-
CHO-K1 NVP2[WYyTnWwY4Tpc44h[XO|YYm= MnLBNVAhdWmwcx?= NXTtbVZzSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQMVU2QTRyLXnu[JVk\WRiW{O1V31IXFCpYX3tZXMh[mmwZHnu[{BqdmO3YnH0[YQh\m:{IEGwJI1qdnNicILpc5IhfG9iQ2CtOVU6PDBvY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDFiaIKgZpkh[mW2YTDjc5VvfGmwZzygT4k:OC5yMEG2{txO MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMES4OEc,OjR7MEC0PFQ9N2F-
HEK293 MYDGeY5kfGmxbjDhd5NigQ>? M{nLbVYxKG2rboO= M3rFfGRqe3CuYXPlcYVvfCCxZjDbN2heW1JzNEG3NVZCKG[xcn2gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZYZ1\XJiNkCgcYlveyCkeTDi[ZRiKGOxdX70bY5oNCCNaU2wMlAxOTkQvF2= NF\5ZmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFQ5PCd-MkS5NFA1QDR:L3G+
CHO-K1 M36zdGZ2dmO2aX;uJIF{e2G7 MlTXOlAhdWmwcx?= NFi5RXFFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tT|Eh[2WubIOgZYZ1\XJiNkCgcYlveyCkeTDi[ZRiKGOxdX70bY5oNCCNaU2wMlUzOs7:TR?= MlfwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC0PFQoRjJ2OUCwOFg1RC:jPh?=
CHO MX\GeY5kfGmxbjDhd5NigQ>? Ml\FNkBpenN? Mo\BSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3LEm0NEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHmeIVzKDJiaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbV0xNjBzMkdOwG0> NYrv[5RFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2PjFpPkK0PVAxPTZzPD;hQi=>
CHO NXjVV3U3TnWwY4Tpc44h[XO|YYm= MnLVNkBpenN? MkC1SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3LEm0NEBnem:vIILlZ49u[mmwYX70JIh2dWGwIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|IHHmeIVzKDJiaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbV0xNjoQvF2= NVL2OYVqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2PjFpPkK0PVAxPTZzPD;hQi=>
CHO NYrP[3JETnWwY4Tpc44h[XO|YYm= MlWyPVAhdWmwcx?= NWHkc|EySWexbnnzeEBi[3Srdnn0fUBifCCJUGK1OUApfW6tbn;3ckBwemmpaX6pJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUFmtbY5lfWOnZDDi[ZRiNWG{cnXzeIlvKHS{YX7zcI9k[XSrb36gZYZ1\XJiOUCgcYlveyCkeTDseY1qdmW|Y3XuZ4Uh[XO|YYmsJGVEPTB;Mj6wNe69VQ>? M1LVeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwOVYyLz5{NEmwNFU3OTxxYU6=
RD-HGA16 MYTGeY5kfGmxbjDhd5NigQ>? MlfaRY51[WexbnnzeEBi[3Srdnn0fUBifCCFQkGtU3gyKGindHXyc4RqdWW{IDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJHJFNUiJQUG2JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2Zib4LlfIlvKEFvaX7keYNm\CClYXzjbZVuKG2xYnnsbZpifGmxbjDifUBndHWxcn;t[ZRzcWNiaX3h[4lv\yCybHH0[UBz\WGmZYKgZY5idHm|aYOsJGtmRTBwMECx{txO MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl2NEezOEc,OjR7NES3N|Q9N2F-
RD-HGA16 NXPVNFNGTnWwY4Tpc44h[XO|YYm= NYXy[lRKSW62YXfvcol{fCCjY4Tpeol1gSCjdDDDRlEhemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iUlStTGdCOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBEWDV3OUSwMYlv\HWlZXSgZ4Ft[2m3bTDtc4JqdGm8YYTpc44h[nliZnz1c5JwdWW2cnnjJIlu[WerbnegdIxifGVicnXh[IVzKGGwYXz5d4l{NCCNZU2wMlAxOTIQvF2= Mnv2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NES3N|QoRjJ2OUS0O|M1RC:jPh?=
Sf9 NULiZmRzTnWwY4Tpc44h[XO|YYm= M1Hw[lIxKG2rboO= MoLrTY53\XK|ZTDh[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHPv[ZhxemW|c3nu[{BI[mW2YUHnZY1u[TJiYX7kJHJUTzRiYYPz[ZN{\WRiYYOg[IVoemGmYYTpc44hd2ZiW3fhcY1iNTN|UG3HWHAh[W[2ZYKgNlAhdWmwczDifUB{fGWjZImtd5RifGViR2TQZZNmKGG|c3H5MEBGSzVyPUCuNFXPxE1? NFq3No89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC5OlI6Pyd-MkWwPVYzQTd:L3G+
Sf9 NIj2[2pHfW6ldHnvckBie3OjeR?= NYmzSmZ[OjBibXnudy=> NHzhV5lKdn[ncoPlJIFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDT[lkh[2WubIOgZ49mgHC{ZYPzbY5oKEekZYThNYdidW2jMjDhcoQhWlOJNDDhd5Nme3OnZDDhd{Bl\We{YXTheIlwdiCxZjDb[4FudWFvM{PQYWdVWCCjZoTldkAzOCCvaX7zJIJ6KHO2ZXHkfU1{fGG2ZTDHWHBie2ViYYPzZZktKEWFNUC9NE4xPTBzMt88US=> M1nKelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEm2Nlk4Lz5{NUC5OlI6PzxxYU6=
CHO NHzpSI1HfW6ldHnvckBie3OjeR?= M4PE[2FvfGGpb37pd5Qh[WO2aY\peJkh[WejaX7zeEBESjGUIDj1cotvd3ewIH;ybYdqdiliQ1jPJINmdGy|IIP0ZYJtgSCneIDy[ZN{cW6pIFfhcJBp[TF4IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2C1OVk1OC2rbnT1Z4VlKGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh[2GuY3n1cUBt\X[nbDDwdoUufHKnYYTl[EAyOCCvaX7zJIJm\m:{ZTDDVFU2QTRyIIP0bY12dGG2aX;uJIJ6KG2rY4LvdIxifGVicnXh[IVzKGKjc3XkJIF{e2G7LDDJR|UxRTBwMEGzOe69VQ>? MoXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNUGyN|EoRjJ4MUWxNlMyRC:jPh?=
CHO MnjySpVv[3Srb36gZZN{[Xl? NHP6RmlCdnSjZ3;ubZN1KGGldHn2bZR6KGGpYXnud5QhS0J{UjCoeY5sdm:5bjDvdolocW5rIFPIU{Bk\WyuczDzeIFjdHliZYjwdoV{e2mwZzDHZYxxcGFzNjDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQOVU6PDBvaX7keYNm\CCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXygdJJmNXS{ZXH0[YQhOTBibXnud{Bj\W[xcnWgR3A2PTl2MDDzeIlufWyjdHnvckBjgSCvaXPyc5Bt[XSnIILlZYRmeiCkYYPl[EBie3OjeTygTWM2OD17LkJOwG0> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF3MUKzNUc,OjZzNUGyN|E9N2F-
HEK293 NI\aUHJHfW6ldHnvckBie3OjeR?= NI\uNY5KdW2nZHnheIUh[W62YXfvcol{fC:Lbo\ldpNmKGGpb37pd5Qh[WO2aY\peJkh[XRiaHXtZYdodHW2aX7pck11[WepZXSgbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhemW4ZYLzZYwhd2ZiMTD1UUBEWDV3LEm0NE1qdmS3Y3XkJIlvcGmkaYTpc44hd2ZiNTD1UUBnd3K|a3;sbY4ucW6mdXPl[EBkSU2SIHHjZ5VufWyjdHnvckBjgSCtaX7leIlkKGODTWCgZZN{[Xl? M3T3XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkCzOlU5Lz5{NkKwN|Y2QDxxYU6=
HEK293 MVrGeY5kfGmxbjDhd5NigQ>? M{PYNFkxKG2rboO= MYLEbZNxdGGlZX3lcpQhd2ZiW{PIYU1EWC13NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEmwJI1qdnNiYomgR49ueGW2aYTpc44h[mmwZHnu[{Bie3OjeTygT4lmeT1yLkCxNu69VQ>? NXTzNJVvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3OVYxQTdpPkK2O|U3ODl5PD;hQi=>
HEK293 NIXxO49HfW6ldHnvckBie3OjeR?= MUm5NEBucW6| MlL4SIl{eGyjY3Xt[Y51KG:oIGuzTH0uS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bi\nSncjC5NEBucW6|IHL5JGNwdXCndHn0bY9vKGKrbnTpcoch[XO|YYmsJGtq\XF;MD63Pe69VQ>? M4nJSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{W2NFk4Lz5{Nke1OlA6PzxxYU6=
CHO-K1 NYjld5c3TnWwY4Tpc44h[XO|YYm= MYTBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1B3NUm0NE1qdmS3Y3XkJIlvfHKjY3XscJVt[XJiY3HsZ4l2dSCvb3LpcIl7[XSrb36gZpkhS2GuY3Xpck01KEGPLYP0ZYlvcW6pIHLhd4VlKE[OSWDSJIF{e2G7LDDL[V0xNjByMUJOwG0> NHH0bI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkiyO|E{Pyd-Mk[4NlcyOzd:L3G+
CHO MlvJSpVv[3Srb36gZZN{[Xl? MUK5NEB{\WO| NWnZeYpnUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh[2GuY3n1cUBud2KrbHn6ZZRqd25iYX\0[ZIhQTBic3Xjd{BjgSCFYXzj[YlvNTRiQV2td5RicW6rbnegZoF{\WRiRlzJVHIh[XO|YYmsJGVEPTB;MD6wNFXPxE1? M4HHVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEK3NVM4Lz5{NkiyO|E{PzxxYU6=
SJ-GBM2 NEjYN3RyUFSVIHHzd4F6 MYHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
A673 Mnv4dWhVWyCjc4PhfS=> MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC NWXuUJc5eUiWUzDhd5NigQ>? M1vMZpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-37 NU\R[ohGeUiWUzDhd5NigQ>? MlS1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy| Mm\uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH M2nLU5FJXFNiYYPzZZk> M{TrRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz M2PIblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MUDxTHRUKGG|c3H5 MXzxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M1TWU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 MkL2dWhVWyCjc4PhfS=> NF7oWY5yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M12zS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 M{L2RZFJXFNiYYPzZZk> M37IUpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= NW\aTFhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
OHS-50 NGfxXHpyUFSVIHHzd4F6 MY\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD MUDxTHRUKGG|c3H5 MonEdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ NEL1flQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 NXPmNVcyeUiWUzDhd5NigQ>? MVXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> NECwSHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MU\xTHRUKGG|c3H5 M4K5XpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? M3PuZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DAOY NUHscYZqeUiWUzDhd5NigQ>? NVnNSVAzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 M3vFcJFJXFNiYYPzZZk> Ml7vdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> NYH5cnZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS NHqzVoxyUFSVIHHzd4F6 NV7xU3JWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 NGq5WopyUFSVIHHzd4F6 MV7xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFQyKGOnbHzz NXuxW3FqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 Ml7qdWhVWyCjc4PhfS=> MYnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> MoS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
CHOK1 M{X1SmZ2dmO2aX;uJIF{e2G7 MVe5NEBucW6| MUPJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgR{11\XKvaX7hcIx6KHC{b3zpcosufGGpZ3XkJI1wfXOnIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPT|Eh[2WubIOgbIFz[m:{aX7nJIJmfGFvZ3HsZYN1d3OrZHHz[UBmdnq7bXWg[pV{\WRiYnX0ZU1ienKnc4TpckBie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDi[ZRiNWG{cnXzeIlvKHKnY4L1bZRu\W62IHHmeIVzKDlyIH3pcpMh[nliY3jlcYltfW2rbnXzZ4VvfCCjc4PhfUwhTUN3ME2wMlAyPc7:TR?= NFXjSHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUmzPVc1PCd-Mkm5N|k4PDR:L3G+
HEK293 MVrGeY5kfGmxbjDhd5NigQ>? M2HMfmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgS3RR\2GvbXHTJIJqdmSrbnegZZN{[XluIFvlQVAvODByMt88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB5N{[wPUc,OzByN{e2NFk9N2F-
HEK293 M4HpOmZ2dmO2aX;uJIF{e2G7 NEC2[3k6OCC|ZXPz MmHWTY53\XK|ZTDh[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5NqdmdiR3HsdIhieTF4IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3HsZ4l2dSCvb3LpcIl7[XSrb36gZYZ1\XJiOUCgd4VkeyCkeTDjZYxk\WmwLUSgRWFOKGS7ZT3iZZNm\CCITFnQVkBie3OjeTygSWM2OD1yLkW4Ou69VQ>? M{DTSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEe3OlA6Lz5|MEC3O|YxQTxxYU6=
HEK293 M1;qe2Z2dmO2aX;uJIF{e2G7 M3;hWlMxKG2rboO= M2HCNmlvfmW{c3WgZYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIZwenOtb3zpck1qdmS3Y3XkJINCVVBiYXPjeY12dGG2aX;uJIFnfGW{IEOwJI1qdnNiYomgTHRTTiCjc4PhfUwhTUN3ME2wMlAxPDIQvF2= MlyzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|M{mzPFcoRjNyM{O5N|g4RC:jPh?=
CHO MX7GeY5kfGmxbjDhd5NigQ>? NITrRWZFcXOybHHj[Y1mdnRib3[gX|NJZS2FUEW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgZ4FvdmGkaX7vbYQhS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nlicnHkbY9tcWejbnSgZolv\GmwZzDhd5NigSxiS3m9NE4yPDQQvF2= NHLaelc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MU[wPVYxQCd-M{G2NFk3ODh:L3G+
CHO MWLGeY5kfGmxbjDhd5NigQ>? NYLo[oxqSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBIWFJ3NTDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBNWElvaX7keYNm\CCkZYThMYFzemW|dHnuJJJm[3K3aYTt[Y51KGK7IHLleIEu\2GuYXP0c5Nq\GG|ZTDlcpp6dWViZoLh[41mdnRiY3;tdIxmdWWwdHH0bY9vKG2ndHjv[EwhTUN3ME2yMlAy|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVEwLz6FaFXNRmw9N2F-
CHO M{fyTWZ2dmO2aX;uJIF{e2G7 MnryRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHVHIyQCCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDi[ZRiNWG{cnXzeIlvKHKnY4L1bZRu\W62IHL5JIJmfGFvZ3HsZYN1d3OrZHHz[UBmdnq7bXWg[pJi\22nboSgZ49ueGynbXXueIF1cW:wIH3leIhw\CxiRVO1NF0yOC5zzszN M12zUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyOS9pPlPoSW1DVDxxYU6=
Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot p-LRP6 / LRP6 / Lgr5 ; β-catenin ; CD44 / Lgr5 / CD133 / EpCAM 29354056
Immunofluorescence Lgr5 ; β-catenin 29354056
In vivo

Rimonabant is administered intraperitoneally or orally potently and dose-dependently antagonize classical pharmacological and behavioural effectos of cannabinoid receptor agonists. [3] In the mouse model of azoxymethane-induced colon carcinogenesis, Rimonabant significantly decreased aberrant crypt foci (ACF) formation, which precedes colorectal cancer. [4] Rimonabant (10 mg/kg by gavage) is fed for 2 weeks to 3-month-old male obese Zucker rats as an impaired glucose tolerance model and for 10 weeks to 6-month-old male obese Zucker rats as a model of the metabolic syndrome. RANTES (Regulated upon Activation, Normal T cell Expressed, and Secreted) and MCP-1 (monocyte chemotactic protein-1) serum levels are increased in obese vs lean Zucker rats and significantly reduced by long-term treatment with Rimonabant, which slowes weight gain in rats with the metabolic syndrome. Neutrophils and monocytes are significantly increased in young and old obese vs lean Zucker rats and lowered by Rimonabant. Platelet-bound fibrinogen is significantly enhanced in obese vs lean Zucker rats of both age, and is reduced by Rimonabant. Platelets from obese rats are more sensitive to thrombin-induced aggregation and adhesion to fibrinogen, which are both attenuated by Rimonabant therapy. [5]

Protocol (from reference)

Kinase Assay:

[1]
  • Radioligand Binding Assay:

    Human CB1 and CB2 stably transfect HEK 293 cells and cell membrane is purified. 0.2-8 μg of the purified membrane is incubated with 0.75 nM [3H] CP55,940 and Rimonabant in the incubation buffer (50 mM Tris-HCl, 5 mM MgCl2, 1 mM EDTA, 0.3%BSA, pH 7.4). The non-specific binding is defined in the presence of 1 μM of CP55,940. The reactions are incubated for one and a half hours at 30 °C in Multiscreen. The reactions are terminated by manifold filtration and washed four times with ice-cold wash buffer (50mM Tris, pH 7.4, 0.25% BSA).The radioactivity bound to the filters is measured by Topcount. The IC50 is determined as the concentration of Rimonabant required to inhibit 50% of the binding of [3H] CP55,940 and calculated by non-linear regression.

Cell Research:

[2]
  • Cell lines: Raw 264.7 cells
  • Concentrations: 0,1,4 μM
  • Incubation Time: 17 hours
  • Method:

    Raw 264.7 cells (2 × 106 /well) in 12-well plates are rinsed with PBS and refed culture media supplemented with varying amounts of Rimonabant 1 hour prior to supplementation with 7-ketocholesterol (7KC). All wells are adjusted to receive equal amounts of vehicle. Following 16-hour incubation, caspase-3 and caspase 3-like activity are determined using a fluorogenic substrate (Ac-DEVD-AFC) and a spectrofluorometer equipped with a microplate reader.
Animal Research:

[3]
  • Animal Models: Male mice and male rats
  • Dosages: 20 ml/kg (mice) and 5 ml/kg (rats)
  • Administration: Rimonabant is administered i.p. (30 minutes) or p.o. (1hour) before the i.v. injection of WIN55212-2.

Solubility (25°C)

In vitro

 DMSO 25 mg/mL
(53.9 mM)
Water Insoluble
Ethanol '''''2 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+5% Tween 80+dd H2O
For best results, use promptly after mixing.

 5 mg/mL

Chemical Information

Molecular Weight 463.79
Formula

C22H21Cl3N4O
CAS No. 168273-06-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(N(N=C1C(=O)NN2CCCCC2)C3=C(C=C(C=C3)Cl)Cl)C4=CC=C(C=C4)Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00750347 Completed Drug: Rimonabant and Paracetamol and placebo Pain University Hospital Clermont-Ferrand September 2008 Phase 1
NCT00656487 Completed Drug: rimonabant|Drug: placebo Cannabis Dependence|Cannabis Withdrawal The Scripps Research Institute|National Institute on Drug Abuse (NIDA) April 30 2008 Phase 2
NCT00546325 Completed Drug: Rimonabant|Drug: Placebo Diabetes Mellitus Type 2 Sanofi October 2007 Phase 3
NCT00525681 Completed Drug: cyclosporine A|Drug: tacrolimus Renal Transplantation University of Oslo School of Pharmacy September 2007 Phase 4
NCT00584389 Terminated Drug: rimonabant|Behavioral: Dietary intervention Obesity University of Surrey|European Foundation for the Study of Diabetes|Royal Surrey County Hospital NHS Foundation Trust July 2007 Phase 4
NCT01041170 Completed Drug: THC|Drug: Rimonobant|Drug: Rimonabant Cannabis|Dependence National Institute on Drug Abuse (NIDA)|National Institutes of Health Clinical Center (CC) April 16 2006 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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